Digital Commons Network^™

The Effect Of Alternative Fertilization And Application Time On Voluntary Intake Of Forages, Jessica Clark

Graduate Theses and Dissertations

Dairy slurry and poultry litter have increased in popularity as fertilizers in the agriculture industry. However, residual effects of these manures on voluntary intake of forages from subsequent harvests are not well known. The objectives of this two part study were to determine if moisture level of alfalfa silage and the use of dairy slurry as a fertilizer have an effect on intake and digestibility by sheep, and if forage species and the use of poultry litter as a fertilizer have an effect on intake by horses. Eighteen ewes (47.6 ± 5.34 kg) were used in experiment 1 and were …

Characterization Of Sbip68: A Putative Tobacco Glucosyltransferase Protein And Its Role In Plant Defense Mechanisms, Abdulkareem O. Odesina

Electronic Theses and Dissertations

Plant secondary metabolites are essential for normal growth and development in plants ultimately affecting crop yield. They play roles ranging from appearance of the plants to defending against pathogen attack and herbivory. They have been used by humans for medicinal and recreational purposes amongst others. Glycosyltransferases catalyze the transfer of sugars from donor substrates to acceptors. Glucosyltransferases are a specific type of glycosyltransferases known to transfer glucose molecules from a glucose donor to a glucose acceptor (aglycone) producing the corresponding glucose secondary metabolite or glycone, in this case glucosides. It was hypothesized that SBIP68, a tobacco putative glucosyltransferase-like protein glucosylated …

Eel River Estuary And Centerville Slough Enhancement Project Historic Resources Addendum, Susie Van Kirk

Susie Van Kirk Papers

A collaborative project-Eel River Estuary and Centerville Slough Enhancement Project-involves governmental agencies, non-profits, and landowners with the goal of restoring and improving habitats and agricultural opportunities on lower Eel River and in the area of Centerville in Humboldt County. Multiple project objectives include restoration of ecosystem functions on a landscape, although altered by diking and ditching, still retains a unique complex of wetlands, sloughs, a major river system, and the Pacific Ocean. Modification of artificial controls such as culverts and tide gates, reconnection of watercourses, sediment reduction, and better management of flooding potential are ongoing projects with more proposed, all …

California Ethnobotany: A Bibliography, James P. Smith Jr

Botanical Studies

No abstract provided.

Distribution Of Herbicide-Resistant Shattercane And Johnsongrass Populations In Sorghum Production Areas Of Nebraska And Northern Kansas, Rodrigo Werle, Amit J. Jhala, Melinda K. Yerka, J. Anita Dille, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Overreliance on acetolactate synthase (ALS)-inhibiting herbicides for weed control during the 1990s resulted in selection of ALS-resistant shattercane [Sorghum bicolor (L.) Moench ssp. drummondii (Nees ex Steud.) de Wet ex Davidse] biotypes in Nebraska. The objective of this study was to assess the baseline presence of ALS-resistance in 190 shattercane and 59 johnsongrass [Sorghum halepense (L.) Pers.] populations collected across northern Kansas, northwestern Missouri, and southern Nebraska in 2013. In 2014, a preliminary field experiment was conducted to evaluate the presence of herbicide resistance in the aforementioned populations. Treatments consisted of four herbicides (clethodim {2-[1-[[(E)-3-chloroprop-2-enoxy]amino] propylidene]-5-(2-ethylsulfanylpropyl)cyclohexane-1,3-dione}, glyphosate [N-(phosphonomethyl) …

Canola: A Modern Crop For A Modern Era, Kenneth J. Roché

Doctor of Plant Health Program: Dissertations and Student Research

The University of Nebraska-Lincoln Doctor of Plant Health program is a professional doctorate program with a comprehensive approach to plants and agriculture. The program emphasizes a broad interdisciplinary education across all plant-related disciplines, practical learning, research, and experience through internships. For my final required internship, I worked as a senior agricultural research intern with Research Designed for Agriculture (RD4AG) in Montana. RD4AG is a contract research organization based in Yuma, AZ with over thirty-years of experience. During my three month internship at RD4AG in Montana, a large portion of my responsibilities centered on managing regulated canola trials that were undertaken …

Soybean Irrigation Initiation Timing Using Evapotranspiration And Soil Moisture Sensor Cues, Justin Chlapecka

Student Theses and Dissertations

Expanded use of irrigation management tools is needed to improve water use efficiency in eastern Arkansas soybean (Glycine max) production. Irrigation initiation timing was studied on a furrow irrigated, sandy loam commercial field in Mississippi County. A major objective was to develop, validate, and expand use of irrigation timing cues, incorporating local weather station data, atmometers, and soil moisture measurements. Four timing treatments were evaluated: early, recommended, late, and rainfed, and the crop and pest response to each. With above average rainfall in 2014 and 2015, results showed little variation of soybean yield. Yield monitor data indicated penalties only within …

Engineering Novel Glycopeptides As A Molecular Carrier For Thermostable E1 Endoglucanase Expressed In Planta, Hong Fang

Student Theses and Dissertations

Reengineering plant cell walls with cell wall-modifying enzymes (CWMEs) represents a promising solution to overcome the recalcitrance for lignocellulosic biomass efficient conversion to liquid biofuels. This project investigated innovative application of hydroxyproline (Hyp)-O-glycosylation to engineer novel glycopeptide tags for heterologous CWMEs to maximize their hydrolytic functions in cell walls without disrupting composition and architecture. I addressed the hypothesis that a strategically designed glycopeptide tag fused to thermostable E1 endoglucanase and expressed in tobacco will facilitate the enzyme deposition into cell wall matrix to make the resulting biomass more subject to biochemical saccharification, while not adversely affecting the normal growth of …

Premature Seed Germination And High Seedling Mortality In A Chaparral Shrub (Adenostoma Fasciculatum) Caused By Severe Drought In Calfiornia, Lexe Evans, Teal Speece, Stephen D. Davis

Biology

The Santa Monica Mountains (SMM) represents one of five Mediterranean-type environments in the world. With sustained summer droughts, this ecosystem is prone to frequent wildfires, a phenomenon that has affected the SMM with regularity once every 32 years. Adenostoma fasciculatum (chamise), is a chaparral shrub well adapted to wildfire cycles because typically its seeds are triggered to germinate by high fire temperatures, smoke, and charate. In the winter of 2015, we were surprised to discover seedlings of A. fasiculatum emerging at an unburned chaparral site without the occurrence of fire. We hypothesized that drought induced canopy dieback at some microsites, …

Recent Dieback In A Keystone Chaparral Species Is Caused By A Fungal Pathogen, Botryosphaeria Dothidea, Adriana Borges, Frida Morales, Emily Pierce, Mariah Taylor

Biology

Due to the recent drought in Southern California, the abundance of a keystone species, Malosma laurina, within the Santa Monica Mountains (SMM) has been dwindling. This species has a rooting depth that exceeds 13 m, so it typically has little difficulty tapping into the water table. Recently, this species has undergone extensive dieback, which might be attributed to the unprecedented drought in California and predisposition fungal pathogen Botryosphaeria. dothidea. While this fungi appears to be innocuous to M. laurina when the plant is healthy, it is more susceptible to invasion under water stress due to the dry and …

Extensive Dieback In Malosma Laurina In Coastal Exposures Of The Santa Monica Mountains Associated With Unprecedented Drought In California, Gina S. Gilderman, Kaitlyn E. Sauer, Gabriella Palmeri

Biology

Throughout coastal exposures of the Santa Monica Mountains (SMM) there is a recent emergence of widespread dieback in Laurel Sumac (Malosma laurina). M. laurina is a keystone species of chaparral that dominates the SMM. A significant trait of M. laurina is an extensive root system exceeding 12 m in depth. We hypothesized that M. laurina was weakened by unprecedented drought in California (2013-2015) and predisposed to fungal pathogens. An initial study conducted in the summer of 2015 revealed all stem samples taken from dieback adult M. laurina plants were infected with Botryosphaeria dothidea, while zero stem samples taken …

Genetic Control Of Morphometric Diversity In The Maize Shoot Apical Meristem, Samuel Leiboff, Xianran Li, Heng-Cheng Hu, Natalie Todt, Jinliang Yang, Xiao Li, Xiaoqing Yu, Gary J. Muehlbauer, Marja C.P. Timmermans, Jianming Yu, Patrick S. Schnable, Michael J. Scanlon

Department of Agronomy and Horticulture: Faculty Publications

The maize shoot apical meristem (SAM) comprises a small pool of stem cells that generate all above-ground organs. Although mutational studies have identified genetic networks regulating SAM function, little is known about SAM morphological variation in natural populations. Here we report the use of high-throughput image processing to capture rich SAM size variation within a diverse maize inbred panel. We demonstrate correlations between seedling SAM size and agronomically important adult traits such as flowering time, stem size and leaf node number. Combining SAM phenotypes with 1.2 million single nucleotide polymorphisms (SNPs) via genome-wide association study reveals unexpected SAM morphology candidate …

Effects Of Control Release Fertilizers On Nutrient Leaching, Palm Growth And Production Cost, Pushpa Soti, Angie Fleurissaint, Stewart Reed, Krish Jayachandran

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Objective of this study was to evaluate the effect of different controlled release fertilizer technologies on nutrient leaching and plant growth parameters of two palm species, Chinese Fan (Livistona chinensis) and Queen (Syagrus romanzoffiana). We compared Nutri-Pak (12-4-12 controlled release packet) and Harrell’s (12-4-12 controlled release polymer coated urea) against Atlantic (8-4-12 controlled release polymer coated urea, coated sulfate of potash), the most commonly used palm fertilizer in South Florida. Plants were grown in 25 cm (11 L) pots under 70% shade, watered weekly, with pest and weed control done as required. Plant growth parameters: number of leaves, leaf length …

Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano

Plant and Soil Sciences Faculty Patents

Compositions and methods for reducing the level of nomicotine and N'-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in …

Residue Harvest Effects On Irrigated, No-Till Corn Yield And Nitrogen Response, Charles S. Wortmann, Charles A. Shapiro, Marty R. Schmer

Department of Agronomy and Horticulture: Faculty Publications

Crop residue harvest occurs on about 40% of Nebraska’s 3,700,000 ha of corn (Zea mays L.) land, primarily for feeding of beef cattle. Immobilization of applied N is expected to be less with residue harvest due to reduced microbial activity for digestion of high C/N ratio organic material. Residue reduction may affect subsequent crop yield and response to applied N. Field research was conducted at three locations over 2 yr in eastern Nebraska for irrigated, no-till corn following corn to determine residue harvest effects on yield and the economically optimal nitrogen rate (EONR). Study sites had deep silt loam …

Evidence Of Field-Evolved Resistance To Bifenthrin In Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Populations In Western Nebraska And Kansas, Adriano E. Pereira, Haichuan Wang, Sarah N. Zukoff, Lance Meinke, B. Wade French, Blair Siegfried

Department of Entomology: Faculty Publications

Pyrethroid insecticides have been used to control larvae or adults of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of field corn in the United States. In response to reports of reduced efficacy of pyrethroids in WCR management programs in southwestern areas of Nebraska and Kansas the present research was designed to establish a baseline of susceptibility to the pyrethroid insecticide, bifenthrin, using susceptible laboratory populations and to compare this baseline with susceptibility of field populations. Concentration-response bioassays were performed to estimate the baseline susceptibility. From the baseline data, a diagnostic concentration (LC99) was determined and …

Herbicides Applied At Or Shortly After Seeding Are Effective For Weed Control In Seedling Buffalograss, Luqi Li, Matthew D. Sousek, Zachary Reicher

Department of Agronomy and Horticulture: Faculty Publications

Herbicides applied shortly after seeding of buffalograss [Buchloe dactyloides (Nutt.) Engelm.] can help reduce weed pressure and maximize establishment of buffalograss. This study evaluated 12 relatively recently developed herbicides for turf safety and weed control when applied at seeding or 0 or 2 weeks after emergence (WAE) of ‘Bowie’ or ‘Sundancer’ buffalograss. Primary weed species on the site were common purslane (Portulaca oleracea L.), redroot pigweed (Amaranthus retroflexus L.), and/or yellow foxtail [Setaria lutescens (Weigel ex Stuntz) F.T. Hubb.]. Regardless of cultivar, untreated checks had53% weed cover by 6 WAE, whereas most of the herbicide treatments resulted …

Synfind: Compiling Syntenic Regions Across Any Set Of Genomes On Demand, Haibao Tang, Matthew D. Bomhoff, Evan Briones, Liangsheng Zhang, James C. Schnable, Eric Lyons

Department of Agronomy and Horticulture: Faculty Publications

The identification of conserved syntenic regions enables discovery of predicted locations for orthologous and homeologous genes, evenwhennosuchgeneispresent.Thiscapabilitymeansthatsynteny-basedmethodsarefarmoreeffectivethansequencesimilaritybased methods in identifying true-negatives, a necessity forstudying gene loss and gene transposition. However, the identification of syntenicregionsrequirescomplexanalyseswhichmustberepeatedforpairwisecomparisonsbetweenanytwospecies.Therefore,as the number of published genomes increases, there is a growing demand for scalable, simple-to-use applications to perform comparative genomic analyses that cater to both gene family studies and genome-scale studies. We implemented SynFind, a web-based tool that addresses this need. Given one query genome, SynFind is capable of identifying conserved syntenic regions in any set of targetgenomes.SynFindiscapableofreportingper-geneinformation,usefulforresearchersstudyingspecificgenefamilies,aswellas genome-wide data sets of syntenic gene and predicted gene …

Population Genetics And Epigenetics Of Two Salt Marsh Plant Species Along An Environmental Gradient, Christy M. Foust

USF Tampa Graduate Theses and Dissertations

Phenotypic plasticity is the ability of a given genotype to exhibit different phenotypes in response to environmental variables, which can impact population level processes. Plasticity of ecologically-relevant traits is important to an organism’s environmental response; however, the underlying mechanisms of plasticity are largely unknown. Ecological epigenetics may offer mechanisms (e.g. DNA methylation) underlying phenotypic plasticity. Epigenetics can be defined as the underlying molecular mechanisms that allow one genotype to exhibit different phenotypes. Differential DNA methylation is one epigenetic mechanism that has been correlated with a number of ecologically-relevant traits; including, differential herbivory in Viola cazorlensis, spinescence in Ilex aquifolium …

A Population Structure And Genome-Wide Association Analysis On The Usda Soybean Germplasm Collection, Nonoy Bandillo, Diego Jarquin, Qijian Song, Randall L. Nelson, Perry Cregan, James Specht, Aaron Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Population structure analyses and genome-wide association studies (GWAS) conducted on crop germplasm collections provide valuable information on the frequency and distribution of alleles governing economically important traits. The value of these analyses is substantially enhanced when the accession numbers can be increased from ~1,000 to ~10,000 or more. In this research, we conducted the first comprehensive analysis of population structure on the collection of 14,000 soybean accessions [Glycine max (L.) Merr. and G. soja Siebold & Zucc.] using a 50KSNP chip. Accessions originating from Japan were relatively homogenous and distinct from the Korean accessions. As a whole, both Japanese and …

Stochastic Models For Plant Microtubule Self-Organization And Structure, Ezgi Can Eren, Ram Dixit, Natarajan Gautam

Biology Faculty Research

One of the key enablers of shape and growth in plant cells is the cortical microtubule (CMT) system, which is a polymer array that forms an appropriately-structured scaffolding in each cell. Plant biologists have shown that stochastic dynamics and simple rules of interactions between CMTs can lead to a coaligned CMT array structure. However, the mechanisms and conditions that cause CMT arrays to become organized are not well understood. It is prohibitively time-consuming to use actual plants to study the effect of various genetic mutations and environmental conditions on CMT self-organization. In fact, even computer simulations with multiple replications are …

Chromatin Immunoprecipitation (Chip) Assay For Detecting Direct And Indirect Protein -Dna Interactions In Magnaporthe Oryzae, Gang Li, Margarita Marroquin-Guzman, Richard A. Wilson

Department of Plant Pathology: Faculty Publications

Chromatin immunoprecipitation (ChIP) is a powerful technology for analyzing protein-DNA interactions in cells. Robust ChIP procedures have been established for investigating direct interactions between protein and DNA. However, detecting indirect protein-DNA interactions in vivo is challenging. Recently, we used ChIP to analyze an indirect protein-DNA interaction between a putative histone demethylase, MoJmjC, and the promoter of the superoxide dismutase 1-encoding gene MoSOD1 in the rice blast fungus Magnaporthe oryzae (M. oryzae) (Fernandez et al., 2014). We tagged MoJmjC with the 3x FLAG epitope (Fernandez et al., 2014), instead of the larger and more commonly used GFP epitope, to mitigate …

Short Day Transcriptomic Programming During Induction Of Dormancy In Grapevine, Anne Fennell, Karen A. Schlauch, Satyanarayana Gouthu, Laurent G. Deluc, Vedbar Khadka, Lekha Sreekantan, Jerome Grimplet, Grant C. Cramer, Katherine L. Mathiason

Agronomy, Horticulture and Plant Science Faculty Publications

Bud dormancy in grapevine is an adaptive strategy for the survival of drought, high and low temperatures and freeze dehydration stress that limit the range of cultivar adaptation. Therefore, development of a comprehensive understanding of the biological mechanisms involved in bud dormancy is needed to promote advances in selection and breeding, and to develop improved cultural practices for existing grape cultivars. The seasonally indeterminate grapevine, which continuously develops compound axillary buds during the growing season, provides an excellent system for dissecting dormancy, because the grapevine does not transition through terminal bud development prior to dormancy. This study used gene expression …

Ua24 Mcicsl Newsletter - November 2015, Shannon R. Trimboli Education Coordinator

MCICSL Newsletter

This issue includes the following:

Partners Recognized for Water Quality Research at Mammoth Cave National Park and Increasing Diversity in the Geosciences

Texas A&M – Galveston Students Participate in Citizen Science at Mammoth Cave National Park

Northern Kentucky University and the Mammoth Cave International Center for Science and Learning to Host Partners in the Parks Program at Mammoth Cave National Park

as well as education and research highlights, and MCICSL symposium information

Enhancing The Effectiveness Of Biological Control Programs Of Invasive Species By Utilizing An Integrated Pest Management Approach, Invasive Species Advisory Committee

National Invasive Species Council

Conclusion

Biological control has been shown on many occasions to be the most cost-effective invasive species management tool available. However, integrating biological control projects with the full breadth of other integrated pest management (IPM) tools, expanding post-re- lease monitoring to maximize efficacy, adaptive management, and incorporating new and innovative ecological, and genetic technologies may provide private and public land managers greater opportunities for long-term success in suppression of established invasive species.

Pengaruh Keberadaan Gulma (Ageratum Conyzoides Dan Boreria Alata) Terhadap Pertumbuhan Dan Hasil Tiga Ukuran Varietas Kedelai (Glycine Max L. Merr) Pada Percobaan Pot Bertingkat, Abdul Karim Kilkoda, Tati Nurmala, Dedi Widayat

Jurnal Kultivasi

Percobaan dilaksanakan di rumah kaca Fakultas Pertanian Universitas Padjadjaran, Jati-nangor, Kabupaten Sumedang, Jawa Barat. Tujuan dari penelitian ini adalah untuk menguji eksudat akar yang terdapat pada gulma Ageratum conyzoides dan Borreria alata terhadap pertumbuhan dan hasil tanaman kedelai. Percobaan dilakukan mulai dari bulan Oktober 2014 sampai dengan bulan Desember 2014 dengan menggunakan rancangan acak kelompok pola faktorial. Faktor perlakuan pertama adalah jenis gulma terdiri dari 3 taraf antara lain gulma A. Conyzoides, gulma B. alata dan tanpa gulma (kontrol). Perlakuan kedua adalah ukuran benih varietas kedelai yang terdiri dari 3 taraf. Total kombinasi perlakuan adalah 9 kombinasi perlakuan, dengan 3 …

Pengaruh Sitokinin Dan Paklobutrazol Terhadap Pertumbuhan Dan Hasil Benih Kentang (Solanum Tuberosum L.) G2 Kultivar Granola Dengan Sistem Nutrient Film Technique, M. Ibrahim, Anne Nuraini, Dedi Widayat

Jurnal Kultivasi

Percobaan ini dilakukan untuk mengetahui pengaruh sitokinin dan paklobutrazol terhadap pertumbuhan dan hasil benih kentang (Solanum tuberosum L.) G2 kultivar Granola dengan menggunakan sistem Nutrient Film Technique. Percobaan dilakukan di rumah plastik CV. Alam Pasundan, Cibiru, Bandung dari bulan April sampai dengan Agustus 2014 dengan ketinggian lokasi penelitian ± 700 m dpl. Rancangan perco-baan yang digunakan adalah Rancangan Acak Kelompok dengan enambelas perlakuan dan masing-masing terdiri dari tiga ulangan. Perlakuan yang digunakan adalah: A (kontrol), B (25 ppm sitokinin), C (50 ppm sitokinin), D (75 ppm sitokinin), E (50 ppm paklobutrazol), F (100 ppm paklobutrazol), G (150 ppm paklobutrazol), H …

Respon Tanaman Mawar Batik (Rosa Hybrida L.) Dengan Penggunaan Konsentrasi 1–Methylcyclopropene (1–Mcp) Pada Beberapa Tingkat Kemekaran Bunga, Yayat Rochayat Suradinata, A. Wulansari

Jurnal Kultivasi

Mawar batik sebagai tanaman hias pot yang berkualitas baik memiliki tajuk yang proposional, ukuran bunga yang seragam dan memiliki kesegaran bunga yang lama, sehingga bunga mawar dapat dinikmati keindahannya lebih lama. Percobaan ini bertujuan untuk memperoleh kombinasi kemekaran bunga dan konsentrasi 1 – Methylcyclopropene (1-MCP) terhadap pertum-buhan dan kualitas bunga pada tanaman mawar batik. Percobaan dilaksanakan sejak Januari 2014 hingga Februari 2014 di greenhouse yang berada di Desa Cihideung, Kabupaten Bandung Barat. Percobaan menggunakan Rancangan Petak Terbagi dengan dua factor, yaitu kemekaran bunga dan dosis 1-MCP, diulang sebanyak tiga kali. Kemekaran bunga yang digunakan yaitu kemekaran 0-10 %, kemekaran 10-25 …

Pengaruh Dosis Herbisida Glifosat Terhadap Gulma, Pertumbuhan, Dan Hasil Tiga Kultivar Kedelai (Glycine Max (L.) Pada Sistem Tanpa Olah Tanah (Tot), Dedi Widayat, R. Ganilda Yustisiyanika

Jurnal Kultivasi

Penelitian ini bertujuan untuk mengetahui pengaruh interaksi antara dosis herbisida Glifosat dan kultivar kedelai terhadap gulma, pertum-buhan, dan hasil tanaman kedelai. Percobaan dilakukan di Kebun Percobaan Saung Meeting Ciparanje Universitas Padjadjaran Jatinangor, Kabupaten Sumedang. Rancangan percobaan yang digunakan adalah rancangan acak kelompok pola faktorial yang terdiri dari dua faktor dan diulang 3 kali. Faktor yang pertama adalah kultivar kedelai yang tediri dari tiga yaitu kultivar Tidar, Burangrang, dan Slamet. Faktor yang kedua dosis herbisida glifosat yang terdiri dari 3 taraf yaitu 1,5l/ha, 3l/ha, dan 4,5l/ha. Kombinasi perlakuan kultivar dan dosis herbisida glifosat tidak memberikan pengaruh interaksi terhadap bobot kering gulma …

Pertumbuhan Dan Perkembangan Tunas Nilam Var. Lhoukseumawe Dari Jenis Eksplan Dengan Sitokinin Yang Berbeda Secara In Vitro, Erni Suminar, Intan Ratna Dewi Anjarsari, Anne Nuraini, Hapizhah

Jurnal Kultivasi

Tujuan dari penelitian ini adalah untuk mendapatan metode perbanyakan tanaman nilam melalui teknik in vitro dengan meng-gunakan jenis eksplan serta jenis dan konsentrasi sitokinin yang berbeda. Percobaan dilaksanakan di Laboratorium Teknologi Benih Fakultas Pertanian Universitas Padjadjaran dari bulan Maret sampai dengan Juni 2012. Rancangan percobaan menggunakan Rancangan Acak Lengkap pola faktorial yang terdiri dua faktor dan tiga kali ulangan. Faktor pertama adalah tipe eksplan (mata tunas, pucuk, dan daun). Faktor kedua adalah tipe dan konsentrasi sitokinin (0 mg L-1, 0,5 mgL-1 BAP, 1,0 mgL-1 BAP0,5 mgL-1 zeatin, and 1,0 mgL-1 zeatin). Hasil percobaan menunjukkan bahwa eksplan pucuk dengan penggunaan 0.5 …