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Comparative Secretome Analysis Suggests Low Plant Cell Wall Degrading Capacity In Frankia Symbionts, Juliana E. Mastronunzio, Louis S. Tisa, Philippe Normand, David R. Benson Jan 2008

Comparative Secretome Analysis Suggests Low Plant Cell Wall Degrading Capacity In Frankia Symbionts, Juliana E. Mastronunzio, Louis S. Tisa, Philippe Normand, David R. Benson

Molecular, Cellular & Biomedical Sciences

Background: Frankia sp. strains, the nitrogen-fixing facultative endosymbionts of actinorhizal plants, have long been proposed to secrete hydrolytic enzymes such as cellulases, pectinases, and proteases that may contribute to plant root penetration and formation of symbiotic root nodules. These or other secreted proteins might logically be involved in the as yet unknown Molecular interactions between Frankia and their host plants. We compared the genome-based secretomes of three Frankia strains representing diverse host specificities. Signal peptide detection algorithms were used to predict the individual secretomes of each strain, and the set of secreted proteins shared among the strains, termed the core …

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Opportunities And Roadblocks In Utilizing Forages And Small Grains For Liquid Fuels, Gautam Sarath, Robert B. Mitchell, S. E. Sattler, Deanna L. Funnell, Jeffrey F. Pedersen, Robert A. Graybosch, Kenneth P. Vogel Jan 2008

Opportunities And Roadblocks In Utilizing Forages And Small Grains For Liquid Fuels, Gautam Sarath, Robert B. Mitchell, S. E. Sattler, Deanna L. Funnell, Jeffrey F. Pedersen, Robert A. Graybosch, Kenneth P. Vogel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This review focuses on the potential advantages and disadvantages of forages such as switchgrass (Panicum virgatum), and two small grains: sorghum (Sorghum bicolor), and wheat (Triticum aesitvum), as feedstocks for biofuels. It highlights the synergy provided by applying what is known from forage digestibility and wheat and sorghum starch properties studies to the biofuels sector. Opportunities therefore, exist to improve biofuel qualities in these crops via genetics and agronomics. In contrast to cereal crops, switchgrass still retains tremendous exploitable genetic diversity, and can be specifically improved to Wt a particular agronomic, management, and conversion …

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The Relationship Between Flower And Fruit Abscission And Alternate Bearing Of ‘Hass’ Avocado, Lauren C. Garner, Carol J. Lovatt Jan 2008

The Relationship Between Flower And Fruit Abscission And Alternate Bearing Of ‘Hass’ Avocado, Lauren C. Garner, Carol J. Lovatt

Horticulture and Crop Science

Despite profuse flowering, ‘Hass’ avocado (Persea americana Mill.) yields are low because of excessive flower and fruit abscission. Whether the dynamics of flower and fruit abscission are influenced by or contribute to alternate bearing, the production of a heavy on-crop followed by a light off-crop that is characteristic of many avocado cultivars, remains unresolved. The objective of this research was to determine whether abscission of reproductive structures from ‘Hass’ avocado trees during specific developmental stages, including flowering, fruit development, and fruit maturity, was influenced by crop status of the current or preceding year. Abscised reproductive structures were collected from commercially …

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Producing Lupins, Peter White, Bob French, Amelia Mclarty, Grains Research And Development Corporation Jan 2008

Producing Lupins, Peter White, Bob French, Amelia Mclarty, Grains Research And Development Corporation

Bulletins 4000 -

The book contains detailed information on lupin establishment, weed control, disease management and harvesting. It also provides an excellent background to the history of lupins in Western Australia, the development of the plant and its adaptation to the Western Australian environment

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Early Season Weed Suppression In Buckwheat Using Clopyralid, Ona Sakaliene, Sharon A. Clay, William C. Koskinen, Gediminas Almantas Jan 2008

Early Season Weed Suppression In Buckwheat Using Clopyralid, Ona Sakaliene, Sharon A. Clay, William C. Koskinen, Gediminas Almantas

Agronomy, Horticulture and Plant Science Faculty Publications

Currently there are no herbicides registered for direct application to buckwheat for broadleaf weed control. This 4-yr Lithuanian study examined weed control using several rates of clopyralid alone or combined with a single rate of desmedipham. Most applications were applied at the 1-leaf stage of crop growth, however, one rate of clopyralid was applied pre-emergence (PRE) in 2 of the 4 yr. Buckwheat injury was evident within a few days after application (or emergence) with all treatments, but by harvest, no symptoms were evident. In the 2 yr with greatest weed densities, densities were reduced with increasing clopyralid rates alone …

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Small Pasture Management Guide, James Barnhill, Scott Mckendrick Jan 2008

Small Pasture Management Guide, James Barnhill, Scott Mckendrick

All Archived Publications

As more people select rural settings for their homes, traditional farms are being divided into small acreage home lots. Many small acreage owners would like to have lush green pastures where they can raise horses, cattle, or sheep. The purpose of this booklet is to provide the educational guidance these owners need to successfully establish and maintain a healthy grazing system.

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2008 Annual Convention Utah Pest And Lawn Care Association, Larry Sagers Jan 2008

2008 Annual Convention Utah Pest And Lawn Care Association, Larry Sagers

All Archived Publications

No abstract provided.

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Algae In Agricultural Fields From St. Francis County, Arkansas, T. Smith Jan 2008

Algae In Agricultural Fields From St. Francis County, Arkansas, T. Smith

Journal of the Arkansas Academy of Science

On August 9th, 2007, two agriculture fields (rice and sorghum fields) were sampled for freshwater algae in St. Francis County. The purpose of this study was to document the algal species in the rice and sorghum fields and compare the similarities of species composition. There were a total of 53 species identified. Overall, diatoms and cyanobacteria were equally dominant with both represented by 21 species (39.6% of the total) and 11 green algal species (20.8%) were present. The sorghum field was dominated by Chlorogloeopsis fritschii and Chroococcus limneticus, while Anabeana cylindrica was abundant in the rice field.

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Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 9 2008, Several Authors Jan 2008

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 9 2008, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

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Instructions For Authors, Discovery Editors Jan 2008

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

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Impact Of Solar Ultraviolet-B On The Proteome In Soybean Lines Differing In Flavonoid Contents, Chenping Xu, Joe H. Sullivan, Wesley M. Garrett, Thomas J. Caperna, Savithiry Natarajan Jan 2008

Impact Of Solar Ultraviolet-B On The Proteome In Soybean Lines Differing In Flavonoid Contents, Chenping Xu, Joe H. Sullivan, Wesley M. Garrett, Thomas J. Caperna, Savithiry Natarajan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was used to systematically investigate the impact of solar ultraviolet- B (UV-B) radiation on the soybean leaf proteome. In order to investigate the protective role of flavonoids against UV-B, two isolines of the Clark cultivar (the standard line with moderate levels of flavonoids and the magenta line with reduced flavonoids) were grown in the field with or without natural levels of UV-B. The 12-day-old first trifoliates were harvested for proteomic analysis. More than 300 protein spots were reproducibly resolved and detected on each gel. Statistical analysis showed that 67 protein spots were significantly (P < 0.05) affected by solar UV-B. Many more spots were altered by UV-B in the magenta line than in the standard line. Another 12 protein spots were not altered by UV-B but showed significantly (P < 0.05) different accumulations between the two lines, and for most spots the line-specific differences were also observed under UV-B exclusion. Most of the differentially accumulated spots were identified by mass spectrometry. The proteins were quite diverse, and were involved in metabolism, energy, protein destination/storage, protein synthesis, disease/defense, transcription, and secondary metabolism. The results suggest that high levels of flavonoids lead to a reduction in UV-B sensitivity at the proteomic level.

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Biotinyl-Methyl 4-(Amidomethyl)Benzoate Is A Competitive Inhibitor Of Human Biotinidase, Keyna Kobza, Kittichai Chaiseeda, Gautam Sarath, James M. Takacs, Janos Zempleni Jan 2008

Biotinyl-Methyl 4-(Amidomethyl)Benzoate Is A Competitive Inhibitor Of Human Biotinidase, Keyna Kobza, Kittichai Chaiseeda, Gautam Sarath, James M. Takacs, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Posttranslational modification of histones by biotinylation can be catalyzed by both biotinidase (BTD) and holocarboxylase synthetase. Biotinylation of histones is an important epigenetic mechanism to regulate gene expression, DNA repair, and chromatin remodeling. The role of BTD in histone biotinylation is somewhat ambiguous, given that BTD also catalyzes removal of the biotin tag from histones. Here, we sought to develop BTD inhibitors for future studies of the role of BTD in altering chromatin structure. We adopted an existing colorimetric BTD assay for use in a novel 96-well plate format to permit high-throughput screening of potential inhibitors. Biotin analogs were chemically …

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Chapter 19. Remediation Of Drinking Water For Rural Populations, W. J. Hunter Jan 2008

Chapter 19. Remediation Of Drinking Water For Rural Populations, W. J. Hunter

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrate is the most common nitrogen contaminant in raw water supplies. In rural areas agricultural activities that involve the use of fertilizers and animal manures are major sources of nitrate contamination. Several processes are currently available that can effectively remove nitrate from raw water. Systems that are suitable for small rural communities include reverse osmosis, ion exchange, and electrodialysis. However, all of these systems move nitrate from raw water and concentrate it in a reject water or brine. Disposal of the reject water can be a major expense as well as an environmental issue. Several emerging systems are under development …

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Auditing And Assessing Air Quality In Concentrated Feeding Operations, N. A. Cole, R. Todd, B. Auvermann, D. B. Parker Jan 2008

Auditing And Assessing Air Quality In Concentrated Feeding Operations, N. A. Cole, R. Todd, B. Auvermann, D. B. Parker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The potential adverse effects of concentrated animal feeding operations (CAFO) on the environment are a growing concern. The air quality issues of most concern to CAFO vary but generally include ammonia, hydrogen sulfide, particulate matter, volatile organic compounds, greenhouse gases, and odors. Air pollutants may be regulated by federal and state laws or by nuisance complaints. The United States Environmental Protection Agency, and poultry, swine, and dairy industries recently agreed to the National Air Emissions Monitoring System to fund research on atmospheric emissions from production farms in the United States. Air quality regulations may be based on actual emissions, atmospheric …

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Evaluation Of Beef Cattle Operations Utilizing Different Seasons Of Calving, Weaning Strategies, Postweaning Management, And Retained Ownership, R. E. Kruse, M. W. Tess, E. E. Grings, R. E. Short, R. K. Heitschmidt, W. A. Phillips, H. S. Mayeux Jan 2008

Evaluation Of Beef Cattle Operations Utilizing Different Seasons Of Calving, Weaning Strategies, Postweaning Management, And Retained Ownership, R. E. Kruse, M. W. Tess, E. E. Grings, R. E. Short, R. K. Heitschmidt, W. A. Phillips, H. S. Mayeux

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Data from a 3-yr study in Montana were utilized to evaluate impacts of season of calving, weaning strategy, and retained ownership of steer calves on enterprise profitability. Calving seasons were late winter (LW), early spring (ES), or late spring (LS). Each season had 2 weaning times: 190 (LW190, ES190) or 240 (LW240, ES240) d for LW and ES, and 140 (LS140) or 190 (LS190) d for LS. Backgrounding options included shipping steers to Oklahoma (OK1), or backgrounding in Montana to a constant age (MT2) or weight (MT3). Steers from OK1 and MT2 were finished in Oklahoma in confinement or via …

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Map-Based Cloning Of An Anthracnose Resistance Gene In Medicago Truncatula, Shengming Yang Jan 2008

Map-Based Cloning Of An Anthracnose Resistance Gene In Medicago Truncatula, Shengming Yang

University of Kentucky Doctoral Dissertations

Anthracnose, caused by the fungal pathogen Colletotrichum trifolii, is one of the most destructive diseases of alfalfa worldwide. Cloning and characterization of the host resistance (R) genes against the pathogen will improve our knowledge of molecular mechanisms underlying host resistance and facilitate the development of resistant alfalfa cultivars. However, the intractable genetic system of cultivated alfalfa, owing to its tetrasomic inheritance and outcrossing nature, limits the ability to carry out genetic analysis in alfalfa. Nonetheless, the model legume Medicago truncatula, a close relative of alfalfa, provides a surrogate for cloning the counterparts of many agronomically important genes in …

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Nitrogen Fertilizer Rate And Weather Dictate Nutritive Value Of Fall Stockpiled Bermudagrass, John A. Guretzky, Jeff Ball, Billy J. Cook Jan 2008

Nitrogen Fertilizer Rate And Weather Dictate Nutritive Value Of Fall Stockpiled Bermudagrass, John A. Guretzky, Jeff Ball, Billy J. Cook

Department of Agronomy and Horticulture: Faculty Publications

Stockpiled bermudagrass [Cynodon dactylon (L.) Pers. ‘Midland’] as a standing forage may meet nutritional needs of beef cows during winter. Our objective was to evaluate accumulation and nutritive value of fall stockpiled bermudagrass in response to N fertilization rate, N application date, and harvest date. Research was conducted near Burneyville, OK from 2000 to 2003. Fertilization rates included 0, 50, 100, and 150 lb N per acre applied on 15 August, 1 September, 15 September, 1 October, and 15 October. Forage accumulation was measured 10 days after the first killing frost; thereafter, crude protein (CP), acid detergent fiber (ADF), …

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In Planta Distribution Of ‘Candidatus Liberibacter Asiaticus’ As Revealed By Polymerase Chain Reaction (Pcr) And Real-Time Pcr, Satyanarayana Tatineni, Uma Shankar Sagaram, Siddarame Gowda, Cecile J. Robertson, William O. Dawson, Toru Iwanami, Nian Wang Jan 2008

In Planta Distribution Of ‘Candidatus Liberibacter Asiaticus’ As Revealed By Polymerase Chain Reaction (Pcr) And Real-Time Pcr, Satyanarayana Tatineni, Uma Shankar Sagaram, Siddarame Gowda, Cecile J. Robertson, William O. Dawson, Toru Iwanami, Nian Wang

Department of Plant Pathology: Faculty Publications

Huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide, and is caused by a phloem-limited fastidious prokaryotic α- proteobacterium that is yet to be cultured. In this study, a combination of traditional polymerase chain reaction (PCR) and real-time PCR targeting the putative DNA polymerase and 16S rDNA sequence of ‘Candidatus Liberibacter asiaticus,’ respectively, were used to examine the distribution and movement of the HLB pathogen in the infected citrus tree. We found that ‘Ca. Liberibacter asiaticus’ was distributed in bark tissue, leaf midrib, roots, and different floral and fruit parts, but not in endosperm and …

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Regulation Of Apical Dominance In Aspergillus Nidulans Hyphae By Reactive Oxygen Species, Camile P. Semighini, Steven D. Harris Jan 2008

Regulation Of Apical Dominance In Aspergillus Nidulans Hyphae By Reactive Oxygen Species, Camile P. Semighini, Steven D. Harris

Department of Plant Pathology: Faculty Publications

In fungal hyphae, apical dominance refers to the suppression of secondary polarity axes in the general vicinity of a growing hyphal tip. The mechanisms underlying apical dominance remain largely undefined, although calcium signaling may play a role. Here, we describe the localized accumulation of reactive oxygen species (ROS) in the apical region of Aspergillus nidulans hyphae. Our analysis of atmA (ATM) and prpA (PARP) mutants reveals a correlation between localized production of ROS and enforcement of apical dominance. We also provide evidence that NADPH oxidase (Nox) or related flavoproteins are responsible for the generation of ROS at hyphal tips and …

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Myron Kendall Brakke: 1921 To 2007, Karen-Beth G. Scholthof, Andrew O. Jackson, James L. Van Etten Jan 2008

Myron Kendall Brakke: 1921 To 2007, Karen-Beth G. Scholthof, Andrew O. Jackson, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Plant pathology lost a distinguished pioneer in plant virology with the death of Dr. Myron K. Brakke on 15 June 2007. Those who were his close colleagues also lost a valued mentor, an irreplaceable friend, and a trusted adviser. Brakke’s most notable accomplishment was the development of sucrose density gradient centrifugation for the purification and characterization of viruses and macromolecules, which led to major advances in biochemistry and molecular biology. He also was responsible for introducing numerous techniques and equipment for fractionation of macromolecules that were crucial for the development of virology, biochemistry, and molecular biology. In the early stages …

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Ultraviolet Light Traps: Design Affects Attraction And Capture, Jerome Hogsette Jan 2008

Ultraviolet Light Traps: Design Affects Attraction And Capture, Jerome Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Traps that use ultraviolet light as an attractant for flies are widely used in urban situations. To determine the differences in trap efficacy from design and lighting, pairs of traps were compared under laboratory conditions. Comparisons were made between traps with open fronts and with traps with restricted open fronts, black light bulbs, and black light blue bulbs, and glue boards with and without z-9-tricosene pheromone. In a windowless laboratory, pairs of traps were placed approximately 90 cm above the floor and 3 m apart. Fifty mixed-sex, 3- to 5-day-old house flies (Musca domestica) were released and …

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Effects Of Agricultural Conservation Practices On Fish And Wildlife Volume 2, Stuart R. Gagnon, Joseph R. Makuch, Cassandra Y. Harper Jan 2008

Effects Of Agricultural Conservation Practices On Fish And Wildlife Volume 2, Stuart R. Gagnon, Joseph R. Makuch, Cassandra Y. Harper

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This bibliography, in two volumes, is part of a multi-volume set developed by the Water Quality Information Center at the National Agricultural Library in support of the United States Department of Agriculture’s Conservation Effects Assessment Project (CEAP). The bibliography is a guide to recent scientific literature covering effects of agricultural conservation practices on fish and wildlife. The citations listed here provide information on how conservation programs and practices designed to improve fish and wildlife habitat, as well as those intended for other purposes, e.g., water quality improvement, affect various aquatic and terrestrial fauna.

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Characterization Of Biological Types Of Cattle: Indicator Traits Offertility In Beef Cows, R. A. Cushman, M. F. Allan, L. A. Kuehn Jan 2008

Characterization Of Biological Types Of Cattle: Indicator Traits Offertility In Beef Cows, R. A. Cushman, M. F. Allan, L. A. Kuehn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Genetic diversity among breeds of cattle allows producers to select animals for specific environments or market conditions. Reproductive efficiency is a multi-component trait that is largely influenced by environmental influences such as health and nutritional status; however, there are clearly genetic components to reproductive efficiency, and breed differences in a number of indicator traits associated with fertility and cow productivity have been identified. Historical indicators of fertility include scrotal circumference, age at puberty, and postpartum interval. Both age at puberty and postpartum interval are laborious traits to collect in heifers and cows because they require many days of detection of …

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Chapter 2. Transformation And Transport Processes Of Nitrogen In Agricultural Systems, Ronald Follett Jan 2008

Chapter 2. Transformation And Transport Processes Of Nitrogen In Agricultural Systems, Ronald Follett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This chapter discusses the transformation and transport processes of nitrogen (N) in agricultural systems and provides information on overall reservoir sizes for N. Nitrogen is ubiquitous in the environment and is required for the survival of all living things. It is also one of the most important essential nutrients and is central to the production of all crop plants. The most abundant form of N in the environment is elemental dinitrogen (N2) gas that accounts for 78% of the atmosphere. N2 gas is inert and is not directly available for plant uptake and metabolism. The atmospheric reservoir …

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Auditing And Assessing Air Quality In Concentrated Feeding Operations, N. A. Cole, R. Todd, B. Auvermann, D. B. Parker Jan 2008

Auditing And Assessing Air Quality In Concentrated Feeding Operations, N. A. Cole, R. Todd, B. Auvermann, D. B. Parker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The potential adverse effects of concentrated animal feeding operations (CAFO) on the environment are a growing concern. The air quality issues of most concern to CAFO vary but generally include ammonia, hydrogen sulfide, particulate matter, volatile organic compounds, greenhouse gases, and odors. Air pollutants may be regulated by federal and state laws or by nuisance complaints. The United States Environmental Protection Agency, and poultry, swine, and dairy industries recently agreed to the National Air Emissions Monitoring System to fund research on atmospheric emissions from production farms in the United States. Air quality regulations may be based on actual emissions, atmospheric …

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Nitrogen Capture By Grapevine Roots And Arbuscular Mycorrhizal Fungi From Legume Cover-Crop Residues Under Low Rates Of Mineral Fertilization, Xiaomei Cheng, Amy Euliss, Kendra Baumgartner Jan 2008

Nitrogen Capture By Grapevine Roots And Arbuscular Mycorrhizal Fungi From Legume Cover-Crop Residues Under Low Rates Of Mineral Fertilization, Xiaomei Cheng, Amy Euliss, Kendra Baumgartner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The influence of mineral fertilization on root uptake and arbuscular mycorrhizal fungi-mediated 15N capture from labeled legume (Medicago polymorpha) residue was examined in winegrapes (Vitis vinifera) in the greenhouse, to evaluate compatibility of fertilization with incorporation of cover-crop residue in winegrape production. Plants grown in marginal vineyard soil were either fertilized with 0.25× Hoagland’s solution or not. This low fertilization rate represents the deficit management approach typical of winegrape production. Access to residue in a separate compartment was controlled to allow mycorrhizal roots (roots + hyphae), hyphae (hyphae-intact), or neither (hyphae-rotated) to proliferate in the …

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Endocrine Responses Of Fast- And Slow-Growing Families Of Channel Catfish, Brian C. Peterson, Brian C. Small, Geoffrey C. Waldbieser, Brian G. Bosworth Jan 2008

Endocrine Responses Of Fast- And Slow-Growing Families Of Channel Catfish, Brian C. Peterson, Brian C. Small, Geoffrey C. Waldbieser, Brian G. Bosworth

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Sixty-eight families of USDA303 channel catfish Ictalurus punctatus were evaluated for growth performance for 30 d. The fastest- and slowest-growing catfish families were further evaluated to examine the hypothesis that genes or gene products associated with the growth regulatory and stress axes can be used to describe differences in growth performance. Research examined mRNA levels of genes involved in the growth hormone–insulin-like growth factor (GH–IGF) network in fast- (family A) and slow-growing (family H) USDA303 catfish. Fish (59.0 ± 2.4 g) were fed for 7 weeks, weighed, and had tissues for RNA extraction. The remaining fish were subjected to an …

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A Gel-Based Reference Map Of The Porcine Hepatocyte Proteome, Thomas J. Caperna, Amy E. Shannon, Wesley M. Garrett Jan 2008

A Gel-Based Reference Map Of The Porcine Hepatocyte Proteome, Thomas J. Caperna, Amy E. Shannon, Wesley M. Garrett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The overall goal of our research is to characterize and identify gene expression profiles of porcine hepatic cells. In this study, we have prepared two-dimensional electrophoresis maps of cytosol and membrane fractions from freshly prepared hepatocytes which were pooled from three crossbred pigs (35–69 kg). Following isoelectric focusing with three pH range immobilized pH gradient strips (pH 3–6, 5–8 and 7–10) and staining the second dimension gels with colloidal Coomassie blue, 728 protein spots were picked and digested with trypsin. Extracted tryptic peptides were initially subjected to matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI–TOF-MS) analysis for identification of proteins …

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Hessian Fly, Jeffrey J. Stuart, Ming-Shun Chen, Marion O. Harris Jan 2008

Hessian Fly, Jeffrey J. Stuart, Ming-Shun Chen, Marion O. Harris

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Hessian fly (Mayetiola destructor) is a gall-forming insect (Harris et al. 2006) with several attributes that make it suitable for genetic analysis: a short life cycle (30 days), a small genome (158 Mb) (Johnston et al. 2004), and polytene chromosomes. The reproductive biology and behavior of the insect also make it an attractive model (Harris and Rose 1989,1990; Bergh et al. 1990; Kanno and Harris 2000; Morris et al. 2000; Harris et al. 2001). Compared to most plant-feeding insects, Hessian flies can be reared in a small space. Unlike gall-forming aphid species, the Hessian fly is always …

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Pastoral Profits Guide, A Paddock Guide To Achieving Sustainable Livestock Productivity, Mark Alchin, Jim Addison, Valarie Shrubb, Zac Cockerill, Matthew Young, Tim Johnson, Greg Brennan Jan 2008

Pastoral Profits Guide, A Paddock Guide To Achieving Sustainable Livestock Productivity, Mark Alchin, Jim Addison, Valarie Shrubb, Zac Cockerill, Matthew Young, Tim Johnson, Greg Brennan

All other publications

Most pastoralists appreciate the need to deliver an economic, environmental and social 'profit' from their business. Achieving these profits can be a real challenge given the comparatively low productive potential and the substantial seasonal variation in the WA Southern Rangelands region. This guide was written to assist pastoralists to meet their specific livestock production, financial and range condition objectives through the better alignment of feed demand (stocking rate) to feed supply (carrying capacity). We provide nine basic steps that simplify the process of managing the feed supply, equipping pastoralists with the tools to make· decisions and to critically assess the …

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