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Integrating High Throughput Phenotyping Approaches To Characterize Plant Traits And Support Nutrient Management Research, Katie J. Bathke

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Over the past decade, considerable research has addressed the social, economic, and agronomic challenges posed by imbalanced nutrient applications, particularly nitrogen and, more recently, sulfur. In response, high throughput plant phenotyping, as one of the latest approaches, has contributed to the development of innovative techniques for assessing key plant phenotypes under stress over time in both field and greenhouse environments. However, there is a limited number of studies applying these techniques for nutrient management, particularly in scenarios where two abiotic stressors coexist, such as the combination of water stress and nitrogen deficiency, or concurrent nitrogen and sulfur deficiencies.

In chapter …

Optimization Of Downy Mildew Management And Baby Kale Production On The Central Coast Of California, Emily M. Locke-Paddon

Master's Theses

Baby kale (Brassica oleracea and other species) is a vital commodity as a salad green in spring mix and is widely produced on the Central Coast of California. Downy mildew, caused by Hyaloperonospora brassicae, is a devastating disease infecting economically important crops like cabbage, broccoli, cauliflower, horseradish, rapeseed, kohlrabi, Brussels sprouts, and kale. This foliar disease causes plant tissue to exhibit chlorotic and necrotic flecking and gray-white sporulation, affecting both the yield and quality of the crop and rendering the affected leaves unmarketable. The Central Coast’s cool climate and nighttime moisture, combined with dense plantings and sprinkler irrigation, …

One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika

All Peer-Reviewed Publications

The global human population is rapidly increasing, escalating interactions of people, animals and the environment. This has led to more convoluted health challenges, for which African smallholder farmers bear the brunt. The One Health (OH) approach recognises the interconnectedness of these health challenges and thus follows a transdisciplinary approach involving diverse stakeholders to address them. Although there have recently been global concerted efforts and studies on OH, an information gap exists in Africa, particularly regarding smallholder farmers and their needs. Additionally, evaluation frameworks and outcome metrics for OH initiatives are still unclear. Thus, this study was conducted to critically map …

To Diversify Or Not To Diversify: A Preliminary Report On Farmers’ Perspectives On Diversification In The U.S. Midwest, Lauren Asprooth, J. Gordon Arbuckle, Rebecca Traldi, Sarah P. Church, Kristin Floress, Benjamin M. Gramig, Andrew J. Margenot, Elizabeth Maynard, Aaron Thompson, Ariana P. Torres, Emily M. Usher, Ishraq Awashra, Katherine Pivaral, Finnleigh S, Woodings, Linda Stalker Prokopy

Department of Horticulture & Landscape Architecture Faculty Publications

Diversifying the simplified landscape of corn and soybeans in the Midwest is an emerging priority in both the public and private sectors to reap a suite of climate, social, agronomic, and economic benefits. However, little research has documented the perspectives of farmers, the primary stakeholders in diversification efforts. This preliminary report uses newly collected survey data (n = 725) from farmers in the states of Illinois, Indiana, and Iowa to provide descriptive statistics and tests to understand what farmers in the region think about agricultural diversification, including their perspectives on its benefits, barriers, and opportunities. For the purposes of the …

Transcriptome Analysis Reveals Nutrient Deprivation Reduces Nitrate Content In Lettuce (Lactuca Sativa Var. Ramosa Hort.) And Enhances Nitrogen Metabolism, Yingying Zhang, Xuena Liu, Shikai La, Mingjiao Wang, Xu Hu, Ainoug Shi, Jinghuai Guo, Lingdi Dong

Horticulture Faculty Publications and Presentations

Introduction Excessive nitrate accumulation in leafy vegetables poses health risks and indicates inefficient nitrogen use in conventional agriculture. While nitrogen metabolism has been extensively studied, the molecular mechanisms linking nutrient deprivation to nitrate reduction in vegetables remain unclear. Methods In this study, we investigated these mechanisms in lettuce (Lactuca sativa var. ramosa Hort.) by analyzing nitrate content and gene expression in leaves and roots under nutrient deprivation. Results After five days of treatment, nitrate content decreased by 57.49% in leaves and 50.77% in roots. Transcriptome sequencing identified 323 and 3,494 differentially expressed genes (DEGs) in leaves and roots, respectively, with …

Evaluation Of Plant Growth Regulators On Sweetpotato Slip Propagation, Kerington Lyn Bass

Theses and Dissertations

Sweetpotato production is often impacted by high mortality of slips during transplantation to the field, particularly with greenhouse-grown slips. These slips develop thinner stems, thus being more vulnerable to transplant shock, resulting in reduced establishment. To address this issue, the study evaluated the effects of four plant growth regulators on the morphological and anatomical characteristics of sweetpotato slips. In the first experiment, external growth parameters were recorded over four weeks, followed by post-harvest assessments of plant and root material. In the second experiment, the widths of various stem structures in stained cross-sections of slip stems were measured. Results showed that …

Molecular Crosstalk Of Transcription Factors And Hormones In Drought Tolerance Genes In Plants, Cengi̇z Kaya

Turkish Journal of Botany

Water deficit is a critical challenge to global agricultural productivity and food security, which is exacerbated by climate change and increasing water scarcity. To withstand drought stress, plants employ a variety of molecular strategies driven by complex regulatory networks. This review examines the central role of transcription factors (TFs) as master regulators that activate drought responsive genes, which in turn mediate key adaptive processes such as osmoregulation, antioxidative defense, and membrane stability. A distinctive aspect of this review is its focus on the dynamic interplay between TFs and hormonal signaling pathways, including abscisic acid, ethylene, salicylic acid, and jasmonic acid. …

Natural Microbial Consortium Construction Strategy From Rhizospheric Agricultural Soils To Improve Wheat (Triticum Durum Desf.) Seedling Growth, Ni̇da Arslan, Mi̇ne Gül Şeker, Özlem Akkaya, Yelda Özden Çi̇ftçi̇

Turkish Journal of Botany

Microbial consortia of compatible microorganisms play a key role in enhancing agricultural sustainability by optimizing nutrient uptake by promoting plant growth more effectively. This study aimed to identify local isolates with the most effective plant growth-promoting (PGP) properties and to form a consortium of isolates showing the optimal synergistic effect between plant and bacterial components to improve the growth of wheat plants. For this purpose, 48 isolates identified from 145 isolates were screened for their PGP activities. According to the results, 16S rRNA gene sequencing was performed to identify the six isolates exhibiting the most promising activity. Different consortium groups …

Genome-Wide Association Of Gene Regions Controlling Selenium And Magnesium Accumulation In Walnuts (Juglans Regia L.), İrem Büyükalgan, Bülent Yağmur, Erdal Orman, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş

Turkish Journal of Botany

Walnut (Juglans regia L.) is one of the most economically important species. It is of great importance to the food industry due to its nutritional value, and high content of many beneficial components such as macro and microelements. In the current study, the aim was to determine markers of single nucleotide polymorphism (SNP) associated with the gene(s) controlling the accumulation of Selenium (Se) and Magnesium (Mg) in walnuts. Se and Mg concentrations were measured in 154 genotypes of Juglans regia in 2021 and 2022. A total of 16,473 SNP markers were used for association mapping to analyze the marker-trait associations. …

Biochar Suppresses Growth, Pupation And Eclosion Success Of A Specialist (Manduca Sexta) And A Generalist (Spodoptera Frugiperda) Insect Herbivore, Nischal Wagle, Soumya Unnikrishnan, Satinderpal Kaur, Engil Pereira, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

Biochar is a charcoal-like substance made by the pyrolysis of organic material from agricultural and forestry waste. While biochar is well documented for altering soil physicochemical conditions, few studies have investigated its possible effects on the management of arthropod pests. Tobacco hornworm (Manduca sexta) and fall armyworm (Spodoptera frugiperda, FAW) are specialist and generalist insect herbivores respectively, that can cause significant defoliation in natural and agricultural ecosystems. In this study, we examined whether walnut shell biochar can affect growth and development of these herbivores. Specifically, we investigated how biochar influences parameters such as mass gain, length of pupation, pupal mass, …

Understanding Temporal Changes In Leaf Traits Of Native Smilax Rotundifolia And Invasive Lonicera Japonica Using Long-Term Herbarium Records From Kentucky, Claudia Ebbecke, Claudia Ebbecke

Honors College Theses

Herbarium specimens are the most valuable, verifiable, and sustainable records to study the impacts of climate change on natural ecosystems. Temporal herbarium data can provide critical information on how species respond to changes in habitat conditions by tracking changes in species’ morphological and phenological traits. This study aims to use Japanese honeysuckle (Lonicera japonica) and common greenbrier (Smilax rotundifolia) to assess the impact of climate change on invasive and native plants. Leaf traits, such as leaf size and stomatal density are good indicators of plant’s adaptation to the changing environment. By examining changes in leaf traits …

Sustainable Process Valorization For Bioactives From Invasive Plants Environmental Burden And Their Efficacy As Antimicrobial Agents, Shamprasad Br

Theses and Dissertations

Agri-food industries produce large quantities of fruit waste which are usually discarded and one such major beverage juice waste is Citrus waste. Likewise, invasive plants like Prosopis juliflora, which is richly distributed across Tamil Nadu, are underutilized for secondary metabolites/bioactives isolation. Halophytes are another rich source of bioactives present around mangrove forests and distributed in the salt rich marshy land.

1. Separation of phenylpropanoids from the Citrus waste through solvent extraction and pH-assisted precipitation was developed. The PPCM precipitated (phenylpropanoids form Citrus medica) were analysed by HPTLC and were found to contain Rutin, Naringin, Quercetin-3-beta-glycoside, Chlorogenic acid, Ferulic acid and optimization …

Comparative Three-Barcode Phylogenetics And Soil Microbiomes Of Planted And Wild Strawberry Trees., Flannery Mclamb, Armando Vazquez, Natalie Olander, Miguel F. Vasquez, Zuying Feng, Niharika Malhotra, Liisa Bozinovic, Karen Najera Ruiz, Katherine O'Connell, Joseph Stagg, Goran Bozinovic

Center for Life in Extreme Environments Publications

Taxonomic identification of closely related plants can be challenging due to convergent evolution, hybridization, and overlapping geographic distribution. To derive taxonomic relationships among planted and wild plants across a large geographic range, we complemented three standard plastid barcodes , , and with soil and fruit chemistry, soil microbiome, and plant morphology analyses. Soil and plant sampling included planted from manicured sites in Southern California, USA, wild plants from Southern and Northern California, and wild populations from Mediterranean island of Hvar, Croatia. We hypothesized that phenotypic variation within and between sites correlates with plants' genotype and geographic distribution. Similar fruit chemistry …

Evaluation Of Tree Legumes Species At Different Agroclimatic Zones Of Indonesia, Mas'ud Panjaitan, R Jessop, W W. Stur

IGC Proceedings (1977-2023)

The population of Indonesia is over 145 million and more than 56 % of them earn their living from agriculture (Bi. Stat. Ind., 1983). A major problem in the development of improved animal production has been the shortage of feed and the generally low nutritive value of tropical forages which limit livestock pro­duction in Indonesia (Asian Agriculture Survey, 1969). The production of feed of adequate quality for ruminants is an important consideration particularly for small holders. In many situations, nitrogen fixing legume trees can be used as a high protein feed supplement which improves the utilization of grasses and crop …

Valeur Nutritive D'Un Parcours Mixte A Acacia Cyanophylla, Atriplex Nummularia Et Medicago Arborea, Y Saadani, C Kayouli

IGC Proceedings (1977-2023)

II existe tres peu de donnees concernant la valeur nutritive des arbustes fourragers ; les rares etudes realisees en Tunisie ont porte surtout sur leur productivite, la charge animale et sur leur appetence (Hamrouni et Sarson, 1975; Ben Boubaker, 1985). L'objectif de ce travail est d'etudier la valeur nutritive des feuilles et brindilles d' Acacia cyanophylla, d' A triplex nummula­ria et de Medicago arborea ainsi que leur contribution dans la ration de caprins et d'ovins.

Forage Production And Nutritive Value Of Leucanea Leucoephala In Southern Puerto Rico, L E. Tergas, R Milton Martinez, A V. Mendez Cruz

IGC Proceedings (1977-2023)

Leucaena leucocephala (L.) is a perennial schrub native to Mexico, Central America and the Caribbean, considered by investigators as a valuable forage legume because of its capacity for high production of digestible forage with a high protein content and tolerance to drought in semiarid regions with annual rainfall less than 1,000 mm (Skerman, 1977). The soil fertility of the semiarid region in southern Puerto Rico is adequate, but rainfall distribution is limiting for forage production and nutritive value. There is a demand for a suit­able and inexpensive source of high quality forage to improve the diet of the grazing cattle …

Apparent Digestibility, Dynamics Of Rumen Fermentation And Sites Of Digestion Of Hays Of Leucaena Leucocephala (Lam) De Wit Cv. Peru, N M. Rodreiguez, I Borges

IGC Proceedings (1977-2023)

Protein deficiency, among others, is a common problem for livestock production in tropical regions. The use oflegumes has been suggested as a solution and Leucaena leucocephala has shown a great potential due to its capacity of nitrogen (N) fix­ation and dry matter (DM) production (NAS, 1984). However, very little is known about its nutritive value regarding its protein-energy balance and the process of digestion. The present work aimed the evaluation of the nutritive value of hays of Leucaena leucocephala cut at two stages of growth, through trials of apparent digestibility, dynamics of rumen fer­mentation and partition of digestion.

In Situ Degradation Of Cell Wall Neutral Sugars Of Normal And Brown Midrib Sorghum X Sudangrass Hybrids, K J. Moore, J O. Fritz, E H. Jaster

IGC Proceedings (1977-2023)

The nutritive value of forage for ruminants is limited by the digestibility of the complex polysaccharides cellulose and hemicellulose by rumen microbes. In grasses, the polysacchar­ide fraction of the cell wall is composed largely of polymers of neutral sugars and to a lesser extent uronic acids. Cellulose is a high molecular weight linear polymer of B-1 ➔ 4) linked D­gluco-pyranose units. Hemicellulose is a complex mixture of linear and branched-chain heteropolymers containing xylose, arabmose, mannose, galactose, glucose, and uronic acids (Van Soest, 1982). Lignification of the cell wall is a primary factor limiting the digestion of cell wall polysaccharides. Close …

Feed Evaluation Using Mobile Dacron Bags And Influences Of The Diet Composition, H Anzola, F N. Owens

IGC Proceedings (1977-2023)

The Mobile Nylon Bag (MNB) technique has been used successfully in protein nutrition studies with swine (Sauer et al., 1983) to determine amino acid availability from protein in specific feedstuffs. Canadian researchers also have tested this technique to predict site of digestion in ruminants (Kirkpatrick and Kennelly, 1987). Differences in small intestinal digestion may explain why animal responses differ to dietary protein sources which have similar ruminal escape values (Owens, 1986). The MNB holds promise to appraise the intestinal avail­ability of undegraded dietary protein though further develop­mel).t and testing are needed. The objective of this experiment was to examine the …

Sites Of Digestion Of Nitrogen Of Hays Made With The Tropical Legume Neonotonia Wightii, R R. Vera, E A. Pizarro, A Toscano, N M. Rodriguez

IGC Proceedings (1977-2023)

Tropical pasture species are of well-known lower quality than temperate species and there is evidence that some tropical legumes may contain antiquality factors that reduce further their nutritive value. Nonetheless, the nutritive value of Neonotonia wightii is generally moderate to high (Pizarro et al., 1983 ; Ribeiro et al., 1980), although there is little information on the factors that affect its quality, and none on the rate and sites of digestion within the gastrointestinal tract of ruminants. The aim of the work reported here was to examine the effect of age on the chemical composition, apparent digestibility and sites of …

Nutritive Value Of Perennial Ryegrass Swards Grazed By Sheep In Autumn In The Uk, J A. Milne, H Dove, A M. Sibbald

IGC Proceedings (1977-2023)

A knowledge of the nutrient supply from grazed herbage for ewes in the autumn in N. Europe is required to predict the consequences for their subsequent reproductive performance. Herbage intake has been found to be positively related to sward height over this period at low sward heights but there is no information over a wider range of heights, on the amounts of non-ammonia nitrogen (NAN) available for absorption in the small intestine nor on the digestion of organic matter in the rumen. Experiments on frozen or artificially dried herbage harvested in the autumn have given variable results for the amounts …

Comparison Of Laboratory Methods For Estimating Herbage Digestibility, Francoise Riviere, P Maupetit, G Blanchard

IGC Proceedings (1977-2023)

Forages are an important source of nutrients for ruminants and recently interest has been shown in using forages for a greater proportion of the nutrients in ruminant production. The feeding value of forages mainly depends on their digestible organic matter contents. Feeding of forages to animals is the most accurate method of evaluation but such trials are expens­ive and very slow. Many laboratory procedures have been developped for esti­mating forage nutritive value. Recently, near infrared reflec­tance has been developped to provide a fast and reliable alter­native method. The aim of the study is to compare laboratory methods of forage evaluation …

Enzymatic Solubility And Other Analytic Parameters Predicting Digestibility In Grasses Of Middling Food Value, J Silva Colomer, M Zarate, J C. Guevara, C Fransinelli

IGC Proceedings (1977-2023)

For a long time, laboratories techniques have been applied to predict "in vivo" digestibility. Among the biological methods, those basing on rumen fluid are the most used in the nutrition laboratories (Osbourn and Siddons, 1980). These techniques, however, present difficulties as the necessity of maintaining rumen-fistulated donor animals, variations in ruminal activity and high cost. For these reasons, the capacity of enzymatic solubility to predict digestibility has been studied in the last few years in different forages : hays, cereal straws, silages and pellets (Aerts et al., 1977 ; Rivero Yeas, 1985), agri­cultural byproducts (Aerts, 1977; Aguilera and Molina, 1986) …

Use Of Near Infrared Spectroscopy To Assess Nutritional Status Of Cattle Diets On Rangelands, J W. Stuth, E D. Kapes, R K. Lyons

IGC Proceedings (1977-2023)

There are two principal means for assessing the quality of grazing ruminant diets, analysis of extrusa from esophageally fistulated animals or indirect estimates from fecal analysis. Wet chemistry methods are well documented for the former but have proven to be of limited value regarding the latter. It is the purpose of this research to determine the suitability of near infrared spectroscopy (NIRS) for estimating diet crude protein (%) and organic matter digestibility (%) directly from extrusa and indirectly from feces.

Near Infrared Reflectance Spectroscopy As An Analytical Tool For World Agriculture, J S. Shenk

IGC Proceedings (1977-2023)

Agriculture produces the food and fiber needed for human existence. In the past 100 years, it becames apparent that nutri­ent content and quantity measurements of feed were necessary in livestock feeding programs. During this 100 year period, scientists developed and refined laboratory reference methods for the industry. However, nutritional evaluation of these products was and still is expensive and time consuming. Near infrared spectroscopy (NIRS) analysis offered the promise of rapid low cost analysis of nutrient composition. This analytical method has the potential to overcome the analytical problems facing world agriculture. First reports of NIRS in the literature occured as …

Monitoring The Nutrition Of Grazing Cattle With Near-Infrared Analysis Of Feces, S W. Coleman, J W. Stuth, J W. Holloway

IGC Proceedings (1977-2023)

The nutrition of grazing animals is difficult to assess because of the difficulty in determining the amount and quality of the ingested forage. Although many techniques have been proposed for sampling the grazed forage, none offer quantitat­ive estimates of diet quality and intake for individual animals in large numbers. The feces excreted by animals contain undigested residues of the diet consumed along with products of animal and microbiological origin. The objective was to determine if near-infrared spectroscopy (NIRS) could be used to developed spectral indices from feces which describe the quantitative and qualitative attributes of the diet over a range …

Prevision De La Valeur Nutritive Des Fourrages Ingeres Sur Parcours Naturels Par Les Ruminants Domestiques Saheliens Et Soudaniens, H Guerin, D Richard, P Lefevre, D Friot, N Mbaye

IGC Proceedings (1977-2023)

Les herbivores domestiques eleves sur Jes parcours naturels saheliens et soudaniens consomment des proportions variables d'especes herbacees annuelles en vegetation pendant 3 a 4 mois puis a l'etat de paille sur pied, ainsi que des feuilles ou des fruits d'especes ligneuses disponibles durant toute l'annee. L'objectif de l'etude est d'etablir des equations de previsions de la digesti­bilite de la matiere organique (DMO) ou de la teneur en matieres azotees digestibles (MAD) de ces fourrages en fonc­tion de leur composition chimique ou de celle des feces des ani­maux qui Jes consomment.

Evaluation Of King Grass Clones (Pennisetum Purpureum) Obtained By Tissue Culture. Iii. Quality Of The Mutants, R S. Herrera, J Lauzan, R O. Martinez, M Monzote, R Cruz

IGC Proceedings (1977-2023)

Two ofthe main aspects of the forages used in bovine cattle nutrition are yield and its quality. More recently, Martinez et al. (1986) observed the possibility of modifying some indices by the in vitro tissue culture. Thus, the objective of the present work was to evaluate some quality indice of Pennisetum clones obtained by tissues culture.

Changes In The Nutritive Value And In The Nutritive Value Yield /Ha Of Some Fodder Grasses During The First Growing Season, D Ferenc

IGC Proceedings (1977-2023)

In Hungary, and also in the areas with similar geographi­cal and climatic conditions, about 40 - 60 per cent of grass yields per year is harvested from the first growth. For the deter­mination of the optimal harvest time it is very important to know the changes in the nutritive value and volume of the first growth. These changes are influenced by the traits of the different varieties too.

Evaluation Of New Varieties Of Fodder Beet (Majoral And Poly Productiva) As New Summer Forages In Egypt, M S. Nowar, S M. Abd El-Baki, I F. Marie, A A. Zaki

IGC Proceedings (1977-2023)

Interest in fodder beet has increased dramatically in many parts of the world. Owing to its yield during summer season in Egypt, time during which shortage of green and succulent for­age occurs, interest in its cultivation started early (Ghoneim, 1967 and Abou El-Hassan et al., 1971). Fodder beet has improved cows condition and milk yield and quality (Kocisova, 1976; Burckhardt, 1979; De Brabander et al., 1981 and Pearce, 1983). Fodder beet can considerably increase the farm total energy production. However, to grow it successfully the farmer must have an « arable» mentality (Pearce, 1983). The present research was conducted to …