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Chapter 19 - Bacteria, Janice M. Glime

Bryophyte Ecology Volume 2: Bryological Interaction

Explore the contents of Bryophyte Ecology, Volume 2, Chapter 19 - Bacteria by clicking the links above. For the Contents section of this ebook, as well as Volumes 1 through 5, please visit the Bryophyte Ecology Main Page.

Ebook sponsored by Michigan Technological University and the International Association of Bryologists.

Nebline, March/April 2022

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Become a Groundwater Steward

4 Ways to Celebrate National Nutrition Month

Recipe of the Month

Selecting Fruit Trees for Eastern Nebraska

Garden Guide: Things to Do This Month

2022 Chemigation Trainings

Lancaster County Ag VIP Awards

Upcoming Early Childhood Trainings

Aleyna Cuttlers Participated in National 4-H Congress

4-H Horse Judging Team at Nationals

Meet 2021–22 4-H Council

Meet 2021–22 4-H Teen Council

Hear of 4-H Volunteer Award: Sherry Kubicek

Hear of 4-H Volunteer Award: Angie Root

4th & 5th Grade Fun Night, March 4

Rabbit Show, March 19

UNL Chick Days, March 26

Bucket Calf Workshop, March 27

Dressage Schooling …

Concentrations Of Digestible, Metabolizable, And Net Energy In Coconut Co-Products Fed To Growing Pigs, Ronilo O. De Castro, Satoshi Ohkura, Akemi Yamamoto, Yasuhiro Morita, Analyn P. Padua, Rommel C. Sulabo

The Philippine Agricultural Scientist

This experiment was conducted to determine the concentration of digestible (DE), metabolizable (ME), and net energy (NE), and to develop prediction equations for DE and ME in coconut co-products (CCP) fed to growing pigs. The CCP used were copra meal (CM), protein enriched copra meal (PECM), and white copra (WC) obtained from the different sources in the Philippines. Twenty-two growing barrows (PIC L337 × C24; initial BW 18.74 ± 0.91 kg) were individually housed in metabolism cages that allowed for a total but separated collection of feces and urine. Pigs were arranged in a replicated 11 × 3 Youden square …

Effect Of Plant Growth Promoting Rhizobacteria And Rhizofungus On The Growth Of Hairy Basil (Ocimum Basilicum L.F. Var. Citratum Back.), Nuntavun Riddech, Tiptida Kidtook, Natthawat Sritongon, Jindarat Ekprasert

The Philippine Agricultural Scientist

In this study, plant growth promoting rhizobacteria (PGPR) and plant growth promoting fungi (PGPF) were isolated from soil adhered to the roots of herbs. PGPR and PGPF isolates were selected for producing microbial inoculant as a starter culture for bio-fertilizer production. The screening of PGPR and PGPF was performed using the spread plate technique on the selected medium. Total plant growth promoting microorganisms (PGPM) were composed of 72 isolates, nitrogen-fixing microorganisms: 39 isolates, phosphate-solubilizing: 11 isolates, and potassium-solubilizing: 22 isolates. Two bacterial isolates, S-K7-2 and S-P7-1, had the highest plant growth promoting abilities, and a fungus isolate, Di-K7-2, was able …

Co-Application Of Phosphorus And Sulfur Improve Yield, Quality, And Nutrients Uptake In Nicotiana Tabaccum L., Aftab Alam, Muhammad Tariq, Jawad Ali, Muhammad Adnan, Shah Fahad, Manzoor Ahmad, Muhammad Romman, Muhammad Hamzad Saleem, Mohammad K. Okla, Shakeel Ahmad, Farhana Gul, Durrishahwar Durrishahwar, Fazli Subhan, Fazli Wahid, Inyat-Ur- Rahman

The Philippine Agricultural Scientist

A field experiment was carried out to evaluate the interactive effect of phosphorus (0, 30, 60, and 90 kg P₂O₅ ha^-1) and sulfur (0, 15, 30, and 45 kg ha^-1) on yield, quality, and uptake of nutrients by tobacco (Nicotiana tabaccum L.). The experiment was organized in a two-factorial randomized complete block design with three replications at Tobacco Research Station, Mardan, Pakistan in 2017-2018. Application of 90 kg P₂O₅ ha^-1 improved tobacco leaf area (24.2%), green leaf weight (44.5%), cured leaf weight (44.7%), grade index (18.3%), nicotine (35.8%), …

Comparative Evaluation Of Physicochemical And Fermentative Responses Of Three Sorghum Varieties From Dryland And Irrigated Land And The Properties Of Proteins From Distillers’ Grains, Jikai Zhao, Thomas Weiss, Zhenjiao Du, Shan Hong, Scott R. Bean, Yonghui Li, Donghai Wang

School of Earth, Environmental, & Marine Sciences Faculty Publications

Three sorghum varieties [waxy sorghum (WS), high protein sorghum (HPS), and normal sorghum (NS)] grown under dryland and irrigated conditions were evaluated with respect to the physicochemical and fermentative properties. The proteins were extracted from wet distillers' grains with solubles (WDGS) and characterized in terms of purity and in vitro digestibility. Starch, crude protein, crude fat, and fiber contents of the sorghums ranged from 64.16 to 68.05 g/100 g, 11.49–13.54 g/100 g, 2.45–2.98 g/100 g, and 1.62–1.80 g/100 g, respectively. For the same variety, the sorghums grown under dryland conditions had higher protein (0.33–0.91 g/100 g) and lower …

Insight On Fruit Fly Ipm Technology Uptake And Barriers To Scaling In Africa, Saliou Niassy, Beatrice Murithii, Evanson R. Omuse, Emily Kimathi, Henri Tonnang, Shepard Ndlela, Samira Mohamed, Sunday Ekesi

All Peer-Reviewed Publications

Tephritid fruit flies are considered one of the world’s most notorious pests of horticultural crops, causing extensive direct and indirect damage. Over the past two decades, a comprehensive, integrated pest management (IPM) package for the management of a plethora of fruit fly pests, includ-ing Bactrocera dorsalis, B. latifrons, B. zonata, Ceratitis cosyra, C. rosa, C. fasciventris, C. quilici, C. capitata, Dacus spp. and Zeugodacus cucurbitae, has been developed, disseminated and promoted among horticultural growers in Africa. To estimate the numbers of beneficiaries reached by the fruit fly IPM technology and the barriers to technology uptake, we interviewed 290 experts in …

Screening And Breeding For Bermudagrass Stem Maggot (Bsm) Resistance Using U.S. Bermudagrass Germplasm, W. F. Anderson, D. Hancock, L. Baxter, W. Hudson, Esteban F. Rios

IGC Proceedings (1977-2023)

Bermudagrass (Cynodon sp.) is an important perennial forage grass grown in many parts of the world. Bermudagrass Stem Maggot (BSM) (Atherigona reversura Villeneuve) is an insect pest that reduces forage yield and nutritive value if it is not controlled. The pest, native to SE Asia, was first documented in North America in 2009 and is now considered invasive. A collection of over 300 forage bermudagrass accessions was evaluated in the field for susceptibility to BSM in 2014 and 2015. Tolerant lines and susceptible checks were then evaluated for yield loss due to BSM in a replicated field study …

Pastoralists’ Perception Of Poverty: A Case Of The Somali And Turkana Of Northern Kenya, W. P. Watete, Kogi-Makau

IGC Proceedings (1977-2023)

Poverty is a complex phenomenon, without a single definition or standard methods of reducing it. Income based methods of assessing poverty have been shown to have limitations that undermine their application in measuring and attacking poverty among pastoral households. Stages of progress method of poverty assessment was used to assess poverty among pastoral households in Turkana and Mandera Counties of Northern Kenya. Three hundred and three hundred and fifty-four households in Mandera and Turkana Counties respectively were sampled across three different livelihood zones: pastoral, agro-pastoral and off-farm. Accumulation of livestock was regarded as a key indicator of wealth among pastoral …

Red Clover Improves The Energy To Protein Balance Of Lucerne-Grass Herbage, X. Godin, Gaëtan F. Tremblay, P. Seguin, S. Bittman, D. Hunt, J. Hakl, G. Bélanger, M.-N. Thivierge, A. Bertrand, M. Thériault, A. Claessens

IGC Proceedings (1977-2023)

Low ratio of readily fermentable carbohydrate to soluble protein concentrations in lucerne (Medicago sativa L.) leads to inefficient use of herbage N by ruminants. To improve the energy to protein balance in lucerne-grass herbage, four proportions of lucerne:red clover (Trifolium pratense L.) were compared in mixtures with and without grasses: timothy (Phleum pratense L.) and tall fescue (Schedonorus arundinaceus Schreb. Dumort.) in Quebec (QC, Canada). In the first post-seeding year, red clover proportion averaged (across grasses and four harvests) 0, 37, 59, and 74% in herbage mixtures. Increasing the proportion of red clover caused a slight …

Structural Features Of Condensed Tannins Influence Their Antimethanogenic Potential In Forage Plants, S. Verma, J.-P. Salminen, S. Wolffram, F. Taube, R. Blank, A. Susenbeth, C. Malisch

IGC Proceedings (1977-2023)

Despite years of research on the antimethanogenic potential of condensed tannins (CT), their large-scale application is inhibited by a substantial variability in previous studies with regards to their impact on ruminant nutrition. This variability mainly results from the complexity of CT structures, and their impact on methane emissions is often unaccounted for. Hence, this study (a) evaluated the variability in antimethanogenic potential across six forage species, (b) linked methane emissions to tannin activity, and (c) determined the impact of CT structural features on methane abatement. Six forage species were grown in a greenhouse under controlled environmental conditions, namely, sainfoin ( …

Round Bale Silage – Farmer Results In Kentucky, Jimmy C. Henning, Jeff Lehmkuhler, Brandon Sears, Corinne Belton, Levi Berg, Don Sorrell, April Wilhoit

Kentucky Alfalfa and Stored Forage Conference

The ability to make silage in round bale packages allows producers to avoid rain damage and produce high quality stored forage. The proliferation of bale wrappers (both individual and inline types) has made this technology more available to producers. Although the process for making baleage is well documented, producers have experienced poor fermentation and in some cases botulism toxicity from baleage. The parameters of good silage are well known and include pH below 5.0 and lactic acid concentrations above 3% on a DM basis. However, tests of farmer produced baleage reveals that often these target values are not achieved. To …

Optimizing Quality With Bale Density And Time Of Wrapping, Jessica Williamson

Kentucky Alfalfa and Stored Forage Conference

No abstract provided.

Forward Of Kentucky Alfalfa And Stored Forages Conference [2022], Jimmy C. Henning, S. Ray Smith, Christopher D. Teutsch

Kentucky Alfalfa and Stored Forage Conference

No abstract provided.

The Role Of Bacteria And Fungi On Forage Degradation In Vitro, Gilberto V. Kozloski, D. C. Rösler, C. A. Pozo, L. E. Leonardi, Mariana P. Mezzomo

IGC Proceedings (1977-2023)

The study was conducted to evaluate the interactive role of bacteria and fungi on forage degradation in vitro. Samples of Cynodon spp. were incubated in a 48-h in vitro gas assay with incubation medium containing or not antimicrobial substances. Treatments were: antibiotic (Ab), antifungal (Af), negative control (i.e. without antimicrobials) or positive control (i.e. with both Ab and Af). Three replicate assays were conducted and, in each assay the gas volume was measured at 3, 6, 9, 12, 24, 36 and 48 h of incubation. Data of cumulative gas production in each flask in each assay was fitted to …

Performance Of Rotational Grazing Of Urochloa Hybrid Cv. Cayman In The Caribbean Region Of Costa Rica, M. Hernández-Chaves, R. Van Der Hoek, S. Abarca-Monge, R. Soto-Blanco, J. Arango

IGC Proceedings (1977-2023)

In Costa Rica pasture-based grass is the basis for both dairy and beef production. The Caribbean region with a rainfall exceeding 3000 mm has four seasons: dry (Feb-May), rainy (May-Aug), less rainy (Sep-Oct) and intermediate (Oct-Feb). Many pastures are dominated by Ischaemum ciliare (“Retana”), of poor nutritional quality and permanently grazed with low stocking rates (one animal per ha). To assess the potential of combining improved grasses with rotational grazing, a trial with Urochloa hybrid cv. Cayman was implemented at the INTA research station in Guápiles, Limón province, during four years (2014 to 2018). An intensive pasture management system based …

Carbon-Sensitive Pedotransfer Functions For Plant Available Water, Dianna K. Bagnall, Cristine L. S. Morgan, Michael Cope, Gregory M. Bean, Shannon Cappellazzi, Kelsey Greub, Daniel Liptzin, Charlotte L. Norris, Elizabeth Rieke, Paul Tracy, Ezra Aberle, Amanda Ashworth, Oscar Bañuelos Tavarez, Andy Bary, Roland Louis Baumhardt, Alberto Borbón Gracia, Daniel Brainard, Jameson Brennan, Dolores Briones Reyes, Darren Bruhjell, Cameron Carlyle, James Crawford, Cody Creech, Steven Culman, William Deen, Curtis Dell, Justin Derner, Thomas Ducey, Sjoerd Willem Duiker, Miles Dyck, Benjamin Ellert, Martin Entz, Avelino Espinosa Solorio, Steven J. Fonte, Simon Fonteyne, Ann-Marie Fortuna, Jamie Foster, Lisa Fultz, Audrey V. Gamble, Charles Geddes, Deirdre Griffin-Lahue, John Grove, Stephen K. Hamilton, Xiying Hao, ‪Zachary D. Hayden, Julie Howe, James Ippolito, Gregg Johnson, Mark Kautz, Newell Kitchen, Sandeep Kumar, Kirsten Kurtz, Francis Larney, Katie Lewis, Matt Liebman, Antonio Lopez Ramirez, Stephen Machado, Bijesh Maharjan, Miguel Angel Martinez Gamiño, William May, Mitchel Mcclaran, Marshall Mcdaniel, Neville Millar, Jeffrey P. Mitchell, Philip A. Moore, Amber Moore, Manuel Mora Gutiérrez, Kelly A. Nelson, Emmanuel Omondi, Shannon Osborne, Leodegario Osorio Alcalá, Philip Owens, Eugenia M. Pena-Yewtukhiw, Hanna Poffenbarger, Brenda Ponce Lira, Jennifer Reeve, Timothy Reinbott, Mark Reiter, Edwin Ritchey, Kraig L. Roozeboom, Ichao Rui, Amir Sadeghpour, Upendra M. Sainju, Gregg Sanford, William Schillinger, Robert R. Schindelbeck, Meagan Schipanski, Alan Schlegel, Kate Scow, Lucretia Sherrod, Sudeep Sidhu, Ernesto Solís Moya, Mervin St. Luce, Jeffrey Strock, Andrew Suyker, Virginia Sykes, Haiying Tao, Alberto Trujillo, Laura L. Van Eerd, Nele Verhulst, Tony John Vyn, Yutao Wang, Dexter Watts, David Wright, Tiequan Zhang, Charles Wayne Honeycutt

Agricultural and Environmental Sciences Faculty Research

Currently accepted pedotransfer functions show negligible effect of management-induced changes to soil organic carbon (SOC) on plant available water holding capacity (θAWHC), while some studies show the ability to substantially increase θAWHC through management. The Soil Health Institute's North America Project to Evaluate Soil Health Measurements measured water content at field capacity using intact soil cores across 124 long-term research sites that contained increases in SOC as a result of management treatments such as reduced tillage and cover cropping. Pedotransfer functions were created for volumetric water content at field capacity (θFC) and permanent wilting point (θPWP). New pedotransfer functions had …

Long-Term Sustainability Implications Of Diverse Commercial Pollinator Mixtures For The Conservation Reserve Program, Amanda J. Ashworth, Taylor Adams, Alayna Jacobs

Plant and Soil Sciences Graduate Research

The persistence of Conservation Reserve Program (CRP) seed mixtures following planting is crucial for the long-term support of pollinator habitat; however, the role of disturbance regimes and their interactions in supporting this ecosystem service are largely unknown. This study set out to evaluate how ecological disturbances (prescribed burn, light disking, or fallow control) and commercially available diverse species mixtures (‘Hamilton’, ‘Bamert’, and ‘Holland’) affect pollinator habitat and the flowering period of commonly used CRP species mixtures. Specifically, three pollinator species mixtures were assessed for plant stand dynamics (plant density, yield, and dual-use pollinator-lignocellulosic feedstock potential); the resulting soil properties; and …

Evaluating Split Timing Fertilizer Applications For Improving Bread Baking Quality Of Soft Red Winter Wheat In Kentucky, Ammar Al Zubade, Krista Jacobsen, Tim Phillips, Mark Williams, David Van Sanford

Plant and Soil Sciences Graduate Research

There is growing interest among farmers to locally produce high protein and strong gluten wheat that is suitable for bread making and meet the demand of local artisanal bakers in Kentucky. The warm and humid weather in southeast region is ideal for soft red winter wheat (SRW) production which characterized by low protein content. The technique of splitting nitrogen (N) fertilization according to the growth stages has been suggested to improve protein content and its composition. This study evaluated the effect of split N application on yield and baking quality traits of two SRW wheat cultivars grown in the eastern …

Effectively Reaching The Equine Community In Kentucky, S. L. Musen, Krista L. Lea, S. Ray Smith, R. J. Coleman

IGC Proceedings (1977-2023)

Since 2009, Extension Specialists and County Extension Agents from the University of Kentucky in the Southeast United States have conducted an annual educational event; the Kentucky Equine Farm and Facilities Expo field-day. These annual field-days have provided practical, hands-on demonstrations and seminars for 135-250 members of the equine community per event. Participants have been a mix of horse owners in relation to farm size, number of horses and business vs. recreation. Many participants have not been previous consumers of Extension educational programming. One of the major draws of the Farm and Facility Expo field days has been the host sites, …

Analysis Of The Attitude Of Farmers Towards Innovations In The Management Of Grasslands In Poland, P. Goliński, A. Van Den Pol-Van Dasselaar, A. Paszkowski, B. Golińska

IGC Proceedings (1977-2023)

Innovations in the management of grasslands in Poland are important to preserve them as a source of different ecosystem services. In the farmers point of view the most promising is forage production for herbivores, particularly dairy cows. Individual farmers have different opinions about innovations. To stimulate innovations in grassland-based farming systems in Poland, it is important to determine the drivers for their promotion and to define the barriers to overcome problems by their implementation. Analysis of the attitude of Polish farmers towards innovations on grasslands were conducted within the H2020 Inno4Grass project. The majority of respondents were young and had …

The Role Of Improved Forages In Solving The Water Scarcity Issue Of 4 Billion People, S. Douxchamps, A. Notenbaert, J. A. Cardoso, M. Romero, M. Peters

IGC Proceedings (1977-2023)

Global animal production requires about 2422 Gm³ of water per year. Most of this volume is used by cattle, with 30% used in the beef sector and another 19% in the dairy sector. At the same time, four billion people suffer from severe water scarcity, raising the flag on diverting an important part of the water globally to livestock production. Livestock-water interactions have therefore gained a prominent profile and fuelled discussions on the water footprint of livestock. A series of studies on the most important components of the livestock water footprint have shown that globally more than 90% of …

Species Richness Increased Yield Stability In Intensively Managed Grasslands Subjected To Experimental Drought, J. A. Finn, M. Suter, E. Haughey, D. Hofer, A. Lüscher

IGC Proceedings (1977-2023)

Climate change is expected to cause an increase in the frequency and intensity of drought events. Over two years we investigated the effects of experimentally imposed drought on intensively managed grassland communities (5 m x 6 m plots) of varying richness (1, 2 and 4 species), and comprising four species (Lolium perenne L., Cichorium intybus L., Trifolium repens L., Trifolium pratense L.). In each year a summer drought period of nine weeks with complete exclusion of precipitation was simulated, inducing severe drought stress at Reckenholz (Zürich, Switzerland), and extreme drought stress at Wexford (Ireland). Mean yield and plot-to-plot variance …

Quantification Of Methane Emissions From Indoor-Fed Fogera Dairy Cows Using Laser Methane Detector, N. Kobayashi, F. Hou, A. Tsunekawa, Tianhai Yan, F. Tegegne, A. Tassew, Y. Mekuriaw, S. Mekuriaw, B. Hunegnaw, W. Mekonnen, T. Ichinohe

IGC Proceedings (1977-2023)

Portable laser methane detectors (LMDs) may be an economical means of estimating CH₄ emissions from ruminants. Here, we validated an LMD-based approach and then used that approach to evaluate CH₄ emissions from indigenous dairy cows in a dryland area of Ethiopia. First, we validated our LMD-based approach in Simmental crossbred beef cattle (n = 2) housed in respiration chambers and fed either a high- or low-concentrate diet. We found that the exhaled air CH₄ concentrations measured by LMD were linearly correlated with the CH₄ emissions determined by infrared-absorption-based gas analyzer (r² = 0.55). …

An Ecophisiological Proposal To Manage Natural Grasslands: A Long Term Trial, F. L. F. De Quadros, D. Roberti, L. Marin, B. Kuinchtner, P. L. Nascimento

IGC Proceedings (1977-2023)

Natural grasslands on Southern Brazil comprise the so called “Rio de La Plata Grasslands” in South America. They are an important fodder source for ruminant pastoral systems and contribute to regional ecosystem services. Strength of these grasslands is its floristic diversity that poses a dilemma to farmers: how to choose management protocols that could be applied for hundreds of species. We propose to use a functional ecophysiological approach based on groups of grasses, the most abundant on aerial biomass of this natural grasslands. We clustered the most frequent grasses in two groups based on its leaf traits (leaf dry matter …

When The Natural Toxin Concentration In Crotalaria Increase In Japan?, M. Kaneko, N. Kato, I. Hattori

IGC Proceedings (1977-2023)

A green manure crop, sunn hemp (Crotalaria juncea L.), legumes, has a nematode-suppressive effect. It is also used as feed in India. However, it is not used as feed in Japan because it contains natural toxins: Pyrrolizidine alkaloids (PAs). PAs has the negative effects for not only livestock but also human health. The PAs, which are abundant in sunn hemp seeds and which are contained only slightly in leaves and stems. Therefore, if sunn hemp harvested before flowering, then no poison problem is posed by PAs. However, if it is harvested at the podding stage, a poisonous concentration of …

Variation Of Pollen Viability In Brachiaria Species And Opportunity In Increased Filled Seed Percentage, Weenaporn Juntasin, Sarayut Thaikua, Rattikan Poungkaew, Pichet Chanpeng, Sompon Waipanya, Yasuhiro Kawamoto

IGC Proceedings (1977-2023)

On pseudogamy process, pollen fertility affects endosperm development in Brachiaria apomictic hybrids. Also, it was well-known that self-incompatibility could affect the fertilization in cross pollination species. Evaluation of pollen viability and percentage of filled seed, and the demonstration of pollen induction technique for increasing filled seed percentage in Brachiaria spp. are presented in this paper. In the first experiment, 8 cultivars/breeding lines of Brachiaria, including 2 open-pollinated cultivars, an apomictic cultivar and 5 hybrids, were planted in randomized completely block design, with 3 replications at northeast Thailand. Each experimental unit consisted of 20 plants, 6 central plants were evaluated. …

Evaluation Of Herbage Intake Estimation Methods For Dairy Cattle Grazing On Semi-Extensive Pastures, S. Perdana-Decker, E. Velasco, J. Werner, U. Dickhoefer

IGC Proceedings (1977-2023)

Available empirical and mechanistic models to estimate total dry matter intake (DMI) and pasture dry matter intake (PDMI) of grazing lactating dairy cows have mainly been developed under intensive grazing conditions. The objective was to evaluate the adequacy of such existent models for use under semi-extensive grazing conditions, characterised by semi-natural grassland and less intensive resource use. Feed intake of lactating cows was measured on three commercial organic dairy farms in South Germany during one or two 6-d-periods/farm in 2019. Each period, DMI was determined in 10 or 20 cows per farm from their daily faecal output measured using titanium …

The Use Of Sunn Hemp As Forage In Florida, João M. B. Vendramini, J. E. Garzon Alfonso, H. M. Da Silva, Maria L. Silveira, J. C. B. Dubeux Jr., M. Kaneko

IGC Proceedings (1977-2023)

Sunn hemp (Crotalaria juncea L.) is a warm-season annual legume that has been widely used as cover crop; however, there is potential to use sunn hemp as forage in subtropical regions. The objective of this study was to evaluate management practices to improve the efficiency of using sunn hemp as a forage crop. The study was conducted in Ona, Florida, USA from 2016 to 2018 and tested the factorial arrangement of five sunn hemp genotypes (Crescent Sunn, Ubon, Blue Leaf, and AU Golden) and two harvest times (60 d after seeding or flowering) with four replicates. There was a …

The Effects Of Microbial Inoculation On Short-To-Long Fermentation And Aerobic Stability Of Grass-Legume Silage Ensiled In Big Bales, J. Jatkauskas, V. Vrotniakiene, K. L. Witt, G. Copani, I. Eisner

IGC Proceedings (1977-2023)

Wilted (35.9 % DM and 2.6% WSC) moderately difficult to ensile grass-legume mixture (red clover, alfalfa and timothy 50:20:30) was ensiled in cylindrical big bales (1.2 m hight and 1.2 m diameter) with a weight about 700 kg. The crop was ensiled for 8, 32 and 120 days with or without a microbial inoculation (SiloSolve^® FC containing Lactococcus lactis DSM 11037/1k2081 and Lactobacillus buchneri DSM 22501/1k20738 applied at 1.5×10⁵ CFU g^-1 forage). Ten bales per treatment were prepared for each fermentation period. After each period of ensiling the big bales were opened, sampled, and tested for fermentation …