Digital Commons Network^™

Intraspecific Hybrid Identification Of Black Walnuts Via Marker Assisted Selection, Makenna B. Thompson

Graduate Theses/Dissertations

The improvement of Eastern black walnut (Juglans nigra L.) cultivars could increase market expansion. Improved cultivars with superior qualities would increase profitability for both the seller and the buyer. Agronomically and economically important traits such as heavier nut weight, disease resistance, tree structure integrity, and yearly nut production can be efficiently improved by breeding black walnuts via marker-assisted selection. Microsatellite/simple sequence repeat markers were used in this research to determine potential intraspecific hybrids between ‘Football’ and ‘Sparrow’ cultivars. Intraspecific is defined as of the same genus and species. This quality makes it more difficult to identify hybrids because the parent …

Morphological And Genetic Analysis Of The Root System In Two American Grapevines (Vitis Species), Parinaz Mohtasebi

Graduate Theses/Dissertations

The North American grapes species Vitis rupestris Scheele and Vitis riparia Michx have been the pillars of rootstock breeding for many decades. Though a large body of viticultural knowledge has been accumulated on their impact on grafted scions, the genetic basis of their root system architecture (RSA) has received limited scientific attention. In this study, I generated and analyzed adventitious root systems from dormant cuttings of 22 V. riparia and 19 V. rupestris accessions, as well as 162 interspecific F1 hybrid progeny from a cross between V. rupestris (♀) and V. riparia (♂). I photographed the roots and then extracted …

Distribution Patterns Of Allorhizobium Vitis In Missouri Vineyards And Non-Vineyard Soils, Jacquelyn M. Wray

Graduate Theses/Dissertations

Crown gall disease causes significant economic loss to the grape and wine industry. Preventive strategies are most effective for mitigating the loss of grapevines in vineyards, as there is no known cure for this disease. The bacterium Allorhizobium vitis carrying a tumor-inducing (Ti) plasmid is the causative agent. A. vitis bacteria live systemically in the grapevine before causing visible symptoms and can survive in residual plant tissues and soil for more than two years. Diagnostic methods have been developed to detect A. vitis bacteria in grapevines and soil. However, more reliable, specific, and high-throughput diagnostics are still needed for screening …

Forage News [2023-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

No abstract provided.

Southeast South Dakota Experiment Farm Annual Progress Report, 2023, Southeast South Dakota Research Farm

Agricultural Experiment Station and Research Farm Annual Reports

This is an annual report of the research program at the Southeast South Dakota Research Farm in cooperation with South Dakota Agricultural Experiment Station and the SDSU College of Agriculture, Food, and Environmental Sciences and has special significance for those engaged in agriculture and the agriculturally related businesses in the ten county area of Southeast South Dakota. The results shown are not necessarily complete or conclusive. Interpretations given are tentative because additional data resulting from continuation of these experiments may result in conclusions different from those based on any one year.

Effects Of Time-Restricted Feeding And Whey Protein Isolate Supplementation On Dietary Intake, Mood, And Sleep In A 12-Week Randomized Controlled Trial, Sydney E. Boudrey, Aubree L. Hawley

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Obesity affects adults in the United States, leading to chronic diseases and reduced well-being. Time-Restricted Feeding (TRF) is a type of dietary intervention lacking current data regarding the effectiveness on facets of well-being. This study’s objective was to determine the effect of time-restricted feeding supplemented with whey protein isolate on food intake, sleep, and mood in overweight or obese adults. Nineteen participants were randomly assigned to the control or experimental group: 1) control, TRF, and 2) experimental, TRF with whey protein supplementation. Participants followed the assigned dietary intervention for 12 weeks. Every 4 weeks (baseline, week 4, week 8, and …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Letter From The Dean, Jean-Francois Meullenet

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Featured Faculty Mentor/Student Team, Gisela Erf, Alessandro Rocchi

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Implications Of Intraspecific Variation In Soil Microbes On Solidago Performance, Yazeed Alshammari

Masters Theses

This study explored the implications of intraspecific variation in soil microbes on the performance of Solidago altissima, a North American species that has become invasive across several continents. This research explored how different clones of Solidago altissima were associated with bacterial and fungal soil microbial communities, which influenced plant performance. In a common garden of 24 Solidago altissima clones, I analyzed the relationship between soil microbial communities, foliar chemistry, clone genetic relatedness, and plant performance. My methods included analysis of soil microbial communities (bacterial and fungal), genetic distances among clones to assess relatedness, and measures of plant performance (light …

Genome-Wide Association Studies And Advanced Genomic Selection Strategies: Towards The Optimization Of Oat (Avena Sativa L) Breeding, Girma Ayana

Electronic Theses and Dissertations

Oats are important cereal crops worldwide, primarily valued for their nutritional and health benefits. The primary goal of oat breeding programs is to accelerate genetic gain to develop oat varieties with the desired product profiles, with the aim of meeting the needs and expectations of growers, processors, and consumers. Achieving this goal involves conducting extensive and resource-intensive field trials, particularly for traits such as crop yield and grain quality, which are strongly influenced by environmental factors. The availability of cost-effective molecular markers and tools like genome-wide association studies (GWAS) and genomic selection have the potential to accelerate the oat breeding …

Profiling The Cell Wall Polysaccharides Of Hemp (Cannabis Sativa L.) Inflorescence And Hempseed And Exploring Their Application As Ruminant Feed Ingredients, Miranda Agbana

Theses and Dissertations--Animal and Food Sciences

Hemp (Cannabis sativa L.) is used in the United States as a source of CBD oil, fiber, and grain. While hempseeds are consumed whole or as dehulled hemp hearts, hempseed oil is also a desirable seed oil in human nutrition. The process of collecting hempseed oil leaves behind hempseed cakes that are abundant in protein and fiber. Feeding of hempseed cakes to animals, including ruminants, has only initially been explored. Extraction of CBD from hemp inflorescences generates organic waste that may find a second purpose as a fiber feed for cattle. Little is known about the exact fiber composition …

Diversity Of Endolichenic Fungi Within Lichen Genus Parmotrema From India, Jyotsna Chakarwarti, Sanjeeva Nayaka, Suchi Srivastava

Turkish Journal of Botany

: The lichens serve as an ecological niche for a group of unexplored fungal species residing inside their thallus without causing any noticeable symptoms and such fungi are termed as endolichenic fungi. The objective of the current study is to check the endolichenic fungal diversity within lichen genus Parmotrema. The study resulted in a total of 450 endolichenic fungi (ELF) isolates from 15 species of Parmotrema. Out of these, 73 sporulating isolates were identified using ITS sequences which resulted in 47 species under 23 genera. The phylogenetic assemblage of the fungi comprised Sordariomycetes (84.50%), Dothideomycetes (5.63%), Eurotiomycetes (7.04%), Pezizomycetes (1.40%), …

Leveraging Data, Models And Farming Innovation To Prevent, Prepare For And Manage Pest Incursions: Delivering A Pest Risk Service For Low-Income Countries, Bryony Taylor, Henri Edouard Zefack Tonnang, Tim Beale, William Holland, Mary Lucy Oronje, Elfatih Mohamed Abdel-Rahman, David Onyango, Cambria Finegold, Jessica Zhu, Stefania Pozzi, Sean T. Murphy

All Peer-Reviewed Publications

Globally, pests (invertebrates, vertebrates, pathogens, weeds) can cause estimated annual losses of between 20% and 40%, but higher losses are disproportionately experienced by many low-income countries, as agriculture is the mainstay of the majority of the people and of national economies. Pests pose a major barrier to these countries’ ability to meet the aims of the UN Sustainable Development Goals (SDGs), particularly SDG2, “End hunger, achieve food security and improved nutrition and promote sustainable agriculture.” However, solutions, in the form of pest risk alert systems, coupled with major advances in technology, are now providing opportunities to overcome this barrier in …

Determining The Effectiveness Of Rosemary Essential Oil On The Shelf Life Of Ground Beef Under Different Lighting Conditions, Jordan T. Looper, Kelly R. Vierck

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

This study determined the effectiveness of rosemary extract on the shelf life of ground beef patties under different retail display conditions. Ground beef patties were produced from an 85%:15% blend (lean:fat). Patties were formed from batches of control or amended with rosemary extract. Patties were individually packaged using overwrap. Groups were assigned into one of two lighting groups (3000K and 3500K). Patties were placed in a simulated retail display for 5 d under continuous lighting and rotated once a day. Lipid oxidation and color samples were taken each day. Relating to lipid oxidation, there was no three-way interaction between display …

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Of Transcription Factor And Transformation: Elucidating Gene Function In The Common Ice Plant, Mesembryanthemum Crystallinum, Chinenye Lilian Izuegbunam

Dissertations and Theses

Abiotic stresses negatively affect plant growth and development across the globe, which also affect the production of food, feed, biofuel, and fiber to meet the demands of a growing population. Thus, developing novel strategies to increase abiotic stress tolerance in crops is necessary. One of these strategies is exploring transcription factors (TFs) in Crassulacean Acid Metabolism (CAM) plants to improve plant abiotic stress tolerances. Therefore, the first objective of this dissertation determine the abiotic stress tolerance function of a CAM TF, McHB7opt in Arabidopsis. This study found that McHB7opt increased stress-responsive pathways related to seed germination and early seedling development. …

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 24 2023, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Exploring The Effects Of Environmental Factors On Rice Blast Disease, April Ellen Lamb

Theses and Dissertations--Plant Pathology

Rice blast disease, caused by the ascomycete fungus Magnaporthe oryzae, occurs in over 85 countries and results in an annual crop loss of 10-30%, a corresponding nutrient value of meals for 60 million people. As a result, it is listed as a critical plant disease by the United Nations. Understanding factors affecting disease severity is of critical concern for food security. M. oryzae has been used as a model system for studying effector-triggered immunity (ETI) by understanding that ETI is primarily a plant response. M. oryzae has been used as a model to study fungal pathogenicity, host specificity, genome …

Propagation, Physiology And Biomass Of Giant Cane (Arundinaria Gigantea) For Conservation And Restoration, Sanjeev Sharm

Graduate Theses/Dissertations

Giant cane (Arundinaria gigantea) is a native species to 22 states in the U.S. The species and its ecosystem are considered critically endangered, and the species has been reduced to 2% of its original extent. The species has a long cultural and conservation history. Large canebrakes were commonly found in Missouri in bottomland forests, stream and riverbanks, and margins of lakes. My research goals were to: 1) examine methods for propagation success from field to greenhouse to field; 2) examine the physiology of cane at one of the few current canebrakes, for greenhouse propagated plants, and field planted …

Effect Of Soil Ph And Indigenous Bacterial Populations In Selecting Rhizobial Endophytes Within Soybean Root Nodules, Brianne Danielle Edwards

Graduate Theses/Dissertations

Soybean plants fulfill most of their nitrogen requirement by developing symbiotic associations with four different rhizobial genera, including Bradyrhizobium, Sinorhizobium, Mesorhizobium, and genus Rhizobium. In general, members of Bradyrhizobium and Sinorhizobium have been reported as dominant root nodule endophytes under acidic soils and alkaline soil conditions, respectively. So far, it is unknown whether the selection of rhizobial endophytes is regulated by their ability to survive under different soil pH or driven by host plants regardless of their relative abundance in soil. This study was focused on the assessment of the potential role of soil pH in …

Fostering Infant And Toddler Music Competence At The Jean Tyson Child Development Study Center, Ellen Mathews, Laura Herold, Shelley Mcnally, Donia Timby

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

This paper presents a creative research project that introduced musical instruments in an outdoor setting to infants and toddlers ages 0-to-3 years old. It was grounded in research suggesting that music plays a vital component in expanding development in the early childhood years, helping to promote learning across many domains. This project began with a survey distributed to 7 infant and toddler classroom educators, after which responses were analyzed for themes regarding perceived infant interests. Subsequent observations were conducted to evaluate the best fit for the implementation of an outdoor experiential music space. Based on the findings, a developmentally appropriate …

Enhancing Tomato Fruit Quality: Application Of Crispr/Dcas9-Mediated Methylation On The Pg Gene Promoter Region, Irena Komninakas

Holster Scholar Projects

Commercially produced tomatoes are typically harvested well before maturation in order to withstand domestic and international distribution. While this process ensures unspoiled goods, the nutritional value of the fruits have been curtailed as a result of premature harvesting. Previous research has found that the polygalacturonase gene (PG gene) is responsible for cell wall degradation of the tomato. In this study, CRISPR/dCas9 technology was employed to increase methylation levels in the promoter regions of the PG gene. This method is significant in that it epigenetically modifies the tomato genome using a technique that has the potential to develop non-genetically modified progeny …

Validation Of A Diagnostic Marker For Primocane-Fruiting In Blackberry, Isabella Vaughn, Alexander Silva, Carmen Johns, Lacy Nelson, Margaret Worthington

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Typical blackberries (Rubus subgenus Rubus) have perennial crowns and roots and biennial canes. The first-year canes (primocanes) are usually vegetative, while second-year canes (floricanes) produce fruit. Primocane-fruiting blackberries produce fruit on first-year canes and are desirable to growers because they potentially allow for a longer harvest season in temperate regions and enable production in tropical areas where no natural chill hours are accumulated. The development of molecular markers for desirable traits can potentially increase efficiency in blackberry breeding. However, to date, there are no diagnostic molecular markers for economically important traits in blackberries. Primocane-fruiting is recessively inherited, and tetraploid blackberries …

Protective Potential Of Mokko Lactone From Cheilocostus Speciosus (J. Koenig) C.D.Specht (Costaceae) Rhizomes Against Fulminant Hepatic Failure, Ahmed Altyar, Ikhlas Sindi, Dina El-Agamy, Wael Elsaed, Ahmed Shehata, Hossam Abdallah, Gamal Mohamed, Abdelsattar Omar, Sabrin Ibrahim

Turkish Journal of Botany

Cheilocostus speciosus (Syn: Costus speciosus) (Crepe or spiral ginger) is one of the species of the family Costaceae that is widely used in various traditional medicines for treating various ailments. Mokko lactone (ML) belongs to the guaianolide family of sesquiterpenes, which has been separated from C. speciosus. It showed noticeable antiinflammation and antioxidative capacities. The current study explored the ML hepatoprotective potential against FHF (fulminant hepatic failure) in mice. FHF was successfully established using intraperitoneal injection of ?-galactosamine (?-GalN, 700 mg/kg)/lipopolysaccharide (LPS, 10 µg/kg) in male Swiss-albino mice. The mice were pretreated with ML (20 or 40 mg/kg, orally) daily …

Changes In Poplar Digestibility Due To Overexpression Of Laccase Genes, Fnu Arunima

Dissertations, Master's Theses and Master's Reports

Woody biomass, particularly poplar, is a sought-after feedstock for lignocellulosic biofuels because of its year-round availability and high productivity. Because of cell wall components like lignin, poplar is inherently resistant to enzymatic saccharification. The experiment aims to determine whether the controlled overexpression of laccase genes alters the biomass composition and whether it can create poplar that is more digestible than the wildtype.

Three sets of transgenic poplar (Populus tremula x alba) samples were evaluated: laccase 18, laccase 27, and STTM. Laccase 18 samples, an target of miRNA397a was overexpressed along with Laccase 27. Short Tandem target Mimic (STTM) technology …

An Inclusive Playground For Infant And Toddler Development, Amanda M. Swartz, Jacquelyn D. Wiersma-Mosley, Donia Timby, Shelley Mcnally, Caitlyn Daniel

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The purpose of this project was to help children reach more developmental goals and to make outdoor play at the Jean Tyson Child Development Study Center more inclusive for all children. Children gain many developmental goals from playing outside and being exposed to other environments as compared to just being inside the classroom. Outdoor play should be as inclusive as indoor play and offer many different activities and outlets, just as the indoor classroom does. The implementation of this service-learning creative project was to add more versatility to the outdoor area at the University of Arkansas Jean Tyson Child Development …

Teaching Agroecology: Preparing Students For Navigating Uncharted Territory, Charles A. Francis, Steve Gliessman

Department of Agronomy and Horticulture: Faculty Publications

Agroecologists understand that farming and food systems are more complex than the aggregation of their components. This realization drives our choices of learning strategies and activities that will prepare students for complexity and uncertainty. Our quest for a just, sustainable, and nutritious food system adequate to equitably serve everyone on the planet today and into the future is an enormous challenge. An undertaking of this magnitude will be met only with major adjustments informed by thoughtful teaching and practicing problem solving skills through a new educational lens. The principles of agroecology help us focus this lens on the wicked problems …

Effect Of Soil Transplantation On Fungal Communities During Restoration, Thomas Luehl

All Master's Theses

Ectomycorrhizal fungi are integral parts of natural ecological systems that provide access to nutrients that would otherwise be unavailable by forming symbiotic relationships with plants. Relationships with ectomycorrhizal fungi are known to improve plant growth rate, increase plant resistance to pathogens, and improve survival. Current revegetation practices often ignore this relationship between fungi and plants. During the revegetation of the Interstate 90 Keechelus Lake wildlife overcrossing (Easton, WA), soil from forest sites adjacent to the restoration area were collected and used to inoculate a set of experimental plots to determine whether ectomycorrhizal fungi would become established in the plots and …

Ascophyllum Nodosum Seaweed Extract Application As A Possible Mechanism For Mitigating Salt Stress In Soybeans (Glycine Max), Elena Hoang

Honors Program Theses

Soil salinity, an abiotic stressor associated with climate change effects, inhibits the normal growth and development of sensitive crop species. The excessive accumulation of salt ions in plant tissue induces oxidative stress, which is characterized by an increased production of reactive oxygen species (ROS) damaging plant organelles and disrupting normal metabolic functions. Seaweed extracts (SWE) may be a solution for mitigating the effects of salt stress on crop yield and quality, as they introduce bioactive ingredients that are able to regulate gene expression and enhance natural physiological processes in plants. However, the positive benefits of SWE applications differ between plant …