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Mpk6, Sphinganine And The Lcb2a Gene From Serine Palmitoyltransferase Are Required In The Signaling Pathway That Mediates Cell Death Induced By Long Chain Bases In Arabidopsis, Mariana Saucedo-García, Arturo Guevara-Garcia, Ariadna González-Solís, Felipe Cruz-García, Sonia Vázquez-Santana, Jennifer E. Markham, M. Guadalupe Lozano-Rosas, Charles R. Dietrich, Maricela Ramos-Vega, Edgar B. Cahoon, Marina Gavilanes-Ruíz

Center for Plant Science Innovation: Faculty Publications

• Long chain bases (LCBs) are sphingolipid intermediates acting as second messengers in programmed cell death (PCD) in plants. Most of the molecular and cellular features of this signaling function remain unknown.

• We induced PCD conditions in Arabidopsis thaliana seedlings and analyzed LCB accumulation kinetics, cell ultrastructure and phenotypes in serine palmitoyltransferase (spt), mitogen-activated protein kinase (mpk), mitogenactivated protein phosphatase (mkp1) and lcb-hydroxylase (sbh) mutants.

• The lcb2a-1 mutant was unable to mount an effective PCD in response to fumonisin B1 (FB1), revealing that the LCB2a gene is essential for the …

A Plant Dj-1 Homolog Is Essential For Arabidopsis Thaliana Chloroplast Development, Jiusheng Lin, Tara J. Nazarenus, Jeanine L. Frey, Xinwen Liang, Mark A. Wilson, Julie M. Stone

Center for Plant Science Innovation: Faculty Publications

Protein superfamilies can exhibit considerable diversification of function among their members in various organisms. The DJ-1 superfamily is composed of proteins that are principally involved in stress response and are widely distributed in all kingdoms of life. The model flowering plant Arabidopsis thaliana contains three close homologs of animal DJ-1, all of which are tandem duplications of the DJ-1 domain. Consequently, the plant DJ-1 homologs are likely pseudo-dimeric proteins composed of a single polypeptide chain. We report that one A. thaliana DJ-1 homolog (AtDJ1C) is the first DJ-1 homolog in any organism that is required for viability. Homozygous disruption of …

Two Distinct Roles Of Arabidopsis Homolog Of Trithorax1 (Atx1) At Promoters And Within Transcribed Regions Of Atx1-Regulated Genes, Yong Ding, Zoya Avramova, Michael E. Fromm

Center for Plant Science Innovation: Faculty Publications

The Arabidopsis thaliana trithorax-like protein, ATX1, shares common structural domains, has similar histone methyltransferase (HMT) activity, and belongs in the same phylogenetic subgroup as its animal counterparts. Most of our knowledge of the role of HMTs in trimethylating lysine 4 of histone H3 (H3K4me3) in transcriptional regulation comes from studies of yeast and mammalian homologs. Little is known about the mechanism by which ATX1, or any other HMT of plant origin, affects transcription. Here, we provide insights into how ATX1 influences transcription at regulated genes, playing two distinct roles. At promoters, ATX1 is required for TATA binding protein (TBP) and …

Structure Function Analysis Of An Adp-Ribosyltransferase Type Iii Effector And Its Rna-Binding Target In Plant Immunity, Byeong-Ryool Jeong, Yan Lin, Anna Joe, Ming Guo, Christin Korneli, Huirong Yang, Ping Wang, Min Yu, Ronald Cerny, Dorothee Staiger, James R. Alfano, Yanhui Xu

Center for Plant Science Innovation: Faculty Publications

Background: HopU1 ADP-ribosylates GRP7, suppressing plant immunity.

Results: The HopU1 structure has two novel loops required for GRP7 recognition, and HopU1 ribosylates GRP7 at an arginine in position 49 disrupting its function.

Conclusion: HopU1 targets a conserved arginine in GRP7, disabling its ability to bind immunity-related RNA.

Significance: The mechanistic details of how HopU1 recognizes its substrate reveal how HopU1 contributes to pathogenesis.

What Controls Toxic Phytoplankton Blooms In Long Island Sound?, Hans G. Dam Ph.D., Michael Finiguerra, Christina Senft-Batoh, Haley Flores

Wrack Lines

TIny phytoplankton organisms known as dinoflagellates can accumulate and cause toxic blooms that can kill fish and even make people ill. It's rare in Long Island Sound but can happen. What drives this phenomenon? Professor Hans G. Dam and his lab team explain in this easy-to-understand article.

The Seed, Justin R. Evertson, Kendall Weyers, Christina Hoyt, Karma Larsen

Nebraska Statewide Arboretum: Publications

People in general seem to harbor a natural fear toward many of the creatures inhabiting our yards and the land- scapes around us. Some of this fear is certainly understand- able, after all, a bee or wasp sting hurts! Others of these creatures are just creepy—spiders, mantids, ants, snakes, bats. If we stop and think about it, however, we’ll soon realize that we really don’t have much to fear and in fact there is much to be gained if we can find a way to better appreciate these creatures. The beautiful goldenrod invites crab spiders and soldier beetles who love …

Functional Hak/Kup/Kt-Like Potassium Transporter Encoded By Chlorella Viruses, Timo Greiner, José Ramos, Maria C. Alvarez, James Gurnon, Ming Kang, James L. Van Etten, Anna Moroni, Gerhard Thiel

James Van Etten Publications

Chlorella viruses are a source of interesting membrane transport proteins. Here we examine a putative K+ transporter encoded by virus FR483 and related chlorella viruses. The protein shares sequence and structural features with HAK/KUP/KT-like K+ transporters from plants, bacteria and fungi. Yeast complementation assays and Rb+ uptake experiments show that the viral protein, termed HAKCV (high-affinity K+ transporter of chlorella virus), is functional, with transport characteristics that are similar to those of known K+ transporters. Expression studies revealed that the protein is expressed as an early gene during viral replication, and proteomics data indicate that it is not packaged in …

Estimating The Potential Returns To Research And Development From Sorghum Value Added Products In El Salvador And Nicaragua, Jaeljattin R. Jaen Celada

INTSORMIL Scientific Publications

Sorghum bicolor (L.) Moench is a drought tolerant crop able to adapt to hot and dry weather. It has excellent chemical and physical properties, which make it a grain of good quality for processing different types of products. This research is an impact assessment study that estimated the potential impacts of new uses of sorghum by using an equilibrium displacement model. The data used was drawn from interviews developed in July 2011.Using total quantity production, prices, prices elasticities and cost shares 8 potential market scenarios were simulated. Results between countries were similar. Thus, the analysis was applied for both countries. …

Exploring The Open-Close Mechanism Of Dimorphotheca Aurantiaca: Day Versus Night And Cold Versus Hot Temperatures, Tommy Chung, Kathleen Aguilar, Sunny Kim

Featured Research

In the spring of every year on Pepperdine University’s campus, patches of hills are covered with the South African native flower Dimorphotheca aurantiaca or more commonly known as the African Daisy. An observation of the opening and closing mechanism of the flower triggered an important question: does the flower change its shape based on temperature or the presence of light? At night, the African Daisy closes and then opens up again in the daytime. Because temperature has such strong effects on different species of ectodermic animals and temperature-sensitive plants, we hypothesized that the change in temperature initiates the flower’s morphological …

Greener Pastures 7 - A Fresh Look At Nutrient Losses From Intensively Managed Pastures, Don Bennett, Bill Russell, Martin Staines, Richard Morris, Mike Bolland, John Lucey

Bulletins 4000 -

Dairy farmers in Western Australia have a long history of being concerned for the environment in which they live and work, from early involvement with Landcare District Committees through to participating in the various programs run in DairyCatch.

They have planted trees, organised soil testing programs, carried out salinity surveys and, more recently, have signed up for effluent, nutrient and irrigation water management programs. Many of these programs produce benefits both on and off the farm—they can improve the farm environment, increase farm productivity and reduce nutrient losses to surface and ground water. The wider community has supported farmers with …

Mojave Applied Ecology Notes Winter 2011, Scott R. Abella, Alice C. Newton, Kenneth Chittick, Pam Sinanian

Mojave Applied Ecology Notes

Lake Mead’s collaborative applied science research program; “annoying the elephant” - ruminations on active management by Alice Newton, Lake Mead NRA vegetation manager; 2012 NWRA Lake Mead symposium sneak peak; assessing forest change using dendrochronology in the Spring Mountains.

Community Structures Of Fecal Bacteria In Cattle From Different Animal Feeding Operations, Orin C. Shanks, Catherine A. Kelty, Shawn Archibeque, Michael Jenkins, Ryan Newton, Sandra L. Mclellan, Susan M. Huse, Mitchell L. Sogin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The fecal microbiome of cattle plays a critical role not only in animal health and productivity but also in food safety, pathogen shedding, and the performance of fecal pollution detection methods. Unfortunately, most published molecular surveys fail to provide adequate detail about variability in the community structures of fecal bacteria within and across cattle populations. Using massively parallel pyrosequencing of a hypervariable region of the rRNA coding region, we profiled the fecal microbial communities of cattle from six different feeding operations where cattle were subjected to consistent management practices for a minimum of 90 days. We obtained a total of …

Twentieth-Century Trends In Essential Fatty Acid Intakes And The Predicted Omega-3 Index: Evidence Versus Estimates, William S. Harris, David M. Klurfeld

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Although the health benefits of omega-3 (n–3) fatty acids are widely appreciated, those of the omega-6 (n–6) fatty acids have become somewhat controversial in recent years, with some viewing them as harmful at current intakes (1, 2) and others (3) [including the American Heart Association (4)] supporting the status quo. Understanding the relations between intakes of these essential fatty acids and disease prevalence over time could inform the debate. To that end, this issue of the Journal contains a report by Blasbalg et al (5) that attempts to reconstruct those intakes and, from this information, to estimate fatty acid tissue …

New Records And Ecological Remarks Regarding The Tribe Stomoxyini (Diptera: Muscidae) From Israel, Günter Müller, Jerome Hogsette, Vasiliy Kravchenko, Edita Revay, Yosef Schlein

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Stomoxyini is a small tribe with only ten genera and 49 known species worldwide (Zumpt 1973) belonging to the Muscidae, one of the largest dipteran. Apart from feeding occasionally on nectar (Müller et al. 2011a), the adult flies of this tribe are obligate blood feeders and can easily be recognized by their conspicuous proboscis adapted for biting and sucking blood (Zumpt 1973). Several members of the tribe, especially Stomoxys and Haematobia species, are major pests of domestic livestock. Two species, Stomoxys calcitrans (Linnaeus 1758) and Haematobia irritans (Linnaeus 1758), cause billions of USD in damages annually in the cattle …

A Novel Monoclonal Antibody Effective Against Lethal Challenge With Swine-Lineage And 2009 Pandemic H1n1 Influenza Viruses In Mice, Hongxia Shao, Jianqiang Ye, Amy L. Vincent, Nicole Edworthy, Andrea Ferrero, Aijian Qin, Daniel R. Perez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The HA protein of the 2009 pandemic H1N1 viruses (H1N1pdm) is antigenically closely related to the HA of classical North American swine H1N1 influenza viruses (cH1N1). Since 1998, through mutation and reassortment of HA genes from human H3N2 and H1N1 influenza viruses, swine influenza strains are undergoing substantial antigenic drift and shift. In this report we describe the development of a novel monoclonal antibody (S-OIV-3B2) that shows high hemagglutination inhibition (HI) and neutralization titers not only against H1N1pdm, but also against representatives of the α, β, and γ clusters of swine-lineage H1 influenza viruses. Mice that received a single intranasal …

Forage News [2011-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forages at KCA
• Small Ruminant Grazing Conference
• Mid-South Stocker Conference
• Kentucky's Agricultural Economy
• Forage Variety Test Results
• Regional Grazing Conference Coming to Louisville - Register Now!
• Alfalfa - Tall Fescue Row Orientation / Compatibility Comparisons on Establishment and Persistence Under Grazing
• Why Graze

Characterization Of Viral And Host Proteins And Rna Elements In Tombusvirus Replication, Kunj Bihari Pathak

Theses and Dissertations--Plant Pathology

Two thirds of plant viruses are positive-strand RNA viruses including the family Tombusviridae. One of the best-studied members of this family is Tomato bushy stunt virus (TBSV). Like many other viruses, TBSV has much fewer genes when compared to its hosts’ genome. Nevertheless, TBSV utilizes its genome very judiciously. To compensate for a lack of many proteins of its own, it codes for multi-functional replication protein p33 and also co-opts host factors to facilitate its replication.

By using recombinant replication proteins p33 and p92 containing single amino acid changes in protein-protein interaction domains (S1 and S2), I demonstrated that the …

Noxious Weed Control: 2011, Mike Moechnig, Leon J. Wrage, Darrell L. Deneke

SDSU Extension Fact Sheets

Noxious weeds are non-native plant species that are a concern to South Dakota land owners and managers. They can seriously impact the native plant community by altering or affecting agriculture, recreation, and wildlife.

Another Really, Really Big Virus, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Viruses with genomes larger than 300 kb and up to 1.2 Mb, which encode hundreds of proteins, are being discovered and characterized with increasing frequency. Most, but not all, of these large viruses (often referred to as giruses) infect protists that live in aqueous environments. Bioinformatic analyses of metagenomes of aqueous samples indicate that large DNA viruses are quite common in nature and await discovery. One issue that is perhaps not appreciated by the virology community is that large viruses, even those classified in the same family, can differ significantly in morphology, lifestyle, and gene complement. This brief commentary, which …

Presence Of Fusarium Spp. In Air And Soil Associated With Sorghum Fields, Deanna L. Funnell-Harris, Jeffrey F. Pedersen

Department of Plant Pathology: Faculty Publications

Sorghum grain, valuable for feed, food, and bioenergy, can be colonized by several Fusarium spp.; therefore, it was of interest to identify possible sources of conidia. Analysis of air and soil samples provided evidence for the presence of propagules from Fusarium genotypes that may cause grain infections. Soil population estimates of members of the Gibberella fujikuroi species complex, that includes sorghum pathogens and other Fusarium spp., suggested that adequate inoculum for systemic infections was present. Conidia in air samples within two sorghum fields were collected by passive trapping for 2 years. Subsampled Fusarium isolates indicated that numbers of G. fujikuroi …

Effects Of Integrating Cultivar Resistance And Fungicide Application On Fusarium Head Blight And Deoxynivalenol In Winter Wheat, Stephen N. Wegulo, William W. Bockus, John Hernandez Nopsa, Erick D. De Wolf, Kent M. Eskridge, Kamaranga H. S. Peiris, Floyd E. Dowell

Department of Plant Pathology: Faculty Publications

Fusarium head blight (FHB) or scab, incited by Fusarium graminearum, can cause significant economic losses in small grain production. Five field experiments were conducted from 2007 to 2009 to determine the effects on FHB and the associated mycotoxin deoxynivalenol (DON) of integrating winter wheat cultivar resistance and fungicide application. Other variables measured were yield and the percentage of Fusarium-damaged kernels (FDK). The fungicides prothioconazole + tebuconazole (formulated as Prosaro 421 SC) were applied at the rate of 0.475 liters/ha, or not applied, to three cultivars (experiments 1 to 3) or six cultivars (experiments 4 and 5) differing in their …

Evaluation Of Hha And Hha Sepb Mutant Strains Of Escherichia Coli O157:H7 As Bacterins For Reducing E. Coli O157:H7 Shedding In Cattle, Vijay K. Sharma, Evelyn A. Dean-Nystrom, Thomas A. Casey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Escherichia coli O157:H7 colonizes cattle intestines by using the locus of enterocyte effacement (LEE)- encoded proteins. The induction of systemic immune response against LEE-encoded proteins, therefore, will prove effective in reducing E. coli O157:H7 colonization in cattle. The previous studies have demonstrated that a hha (encodes for a hemolysin expression modulating protein) deletion enhances expression of LEE-encoded proteins and a sepB (encodes an ATPase required for the secretion of LEE-encoded proteins) deletion results in intracellular accumulation of LEE proteins. In this study, we demonstrate the efficacy of the hha and hha sepB deletion mutants as bacterins for …

New Records For The Horse Fly Fauna (Diptera: Tabanidae) Of Jordan With Remarks On Ecology And Zoogeography, Günter Müller, Jerome Hogsette, Edita Revay, Vasiliy Kravchenko, Andrey Leshvanov, Yosef Schlein

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The horse fly fauna (Diptera: Tabanidae) of Jordan is, after Israel, the richest in the Levant, with 24 known species. During the 20-year project “The Ecology and Zoogeography of the Lepidoptera of the Near East,” we regularly collected blood-feeding flies, resulting in 11 additional species of Tabanidae for Jordan. The new records are: Atylotus quadrifarius (Loew, 1874), Chrysops caecutiens (Linnaeus, 1758), Dasyrhamphis nigritus (Fabricius, 1794), Haematopota pallens Loew, 1871, Nemorius irritans (Ricardo, 1901), Philipomyia graeca (Fabricius, 1794), Tabanus cordiger Meigen, 1820, Tabanus taeniola Palisot de Beauvois, 1806, Tabanus quatuornotatus Meigen, 1820, Tabanus separatus Effllatoun, 1930, and Tabanus spectabilis Loew, 1858. …

Vector Competence Of The Stable Fly (Diptera: Muscidae) For West Nile Virus, Michael S. Doyle, Bethany N. Swope, Jerome A. Hogsette, Kristen L. Burkhalter, Harry M. Savage, Roger S. Nasci

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In 2006-2007, stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae), were suspected of being enzootic vectors of West Nile virus (family Flaviviridae, genus Flavivirus, WNV) during a die-off of American white pelicans (Pelecanus erythrorhynchos Gmelin) (Pelecanidae) in Montana, USA. WNV-positive stable flies were observed feeding en masse on incapacitated, WNV-positive pelicans, arousing suspicions that the flies could have been involved in WNV transmission among pelicans, and perhaps to livestock and humans. We assessed biological transmission by infecting stable flies intrathoracically with WNV and testing them at 2-d intervals over 20 d. Infectious WNV was detected in fly bodies …

Dietary Wheat Germ Oil Influences Gene Expression In Larvae And Eggs Of The Oriental Fruit Fly, Thomas A. Coudron, Chiou Ling Chang, Cynthia L. Goodman, David Stanley

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Changes in animal nutrition, particularly essential dietary components, alter global gene expression patterns. Our goal is to identify molecular markers that serve as early indicators of the quality of insect culture media. Markers of deficient culture media will increase the efficiency of developing optimal systems for mass rearing beneficial insects and some pest species because decisions on culture media quality can be made without waiting through one or several life cycles. The objective of our current study is to discover molecular markers of essential dietary lipid deficiency in the oriental fruit fly, Bactrocera dorsalis. We reared groups of fruit …

Uncertainty In Modelling Of Faecal Coliform Overland Transport Associated With Manure Application In Maryland, A. K. Guber, Y. A. Pachepsky, A. M. Yakirevich, D. R. Shelton, A. M. Sadeghi, D. C. Goodrich, C. L. Unkrich

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Concerns for microbial safety of surface water facilitate development of predictive models that estimate concentrations and total numbers of pathogen and indicator organisms leaving manure-fertilized fields in overland flow during runoff events. Spatial variability of bacterial concentrations in applied manure introduces high uncertainty in the model predictions. The objective of this work was to evaluate the uncertainty in model predictions of the manure-borne bacteria overland transport caused by limited information on the spatial distribution of bacteria in surface-applied manure. Experiments were carried out at the ARS Beltsville experimental watershed site (OPE3) in Maryland. Dairy bovine manure was applied at a …

Outcrossing And Crossbreeding Recovers Deteriorated Traits In Laboratory Cultured Steinernema Carpocapsae Nematodes, John M. Chaston, Adler R. Dillman, David I. Shapiro-Ilan, Anwar L. Bilgrami, Randy Gaugler, Keith R. Hopper, Byron J. Adams

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The nematode Steinernema carpocapsae infects and kills many pest insects in agro-ecosystems and is commonly used in biocontrol of these pests. Growth of the nematodes prior to distribution for biocontrol commonly results in deterioration of traits that are essential for nematode persistence in field applications. To better understand the mechanisms underlying trait deterioration of the efficacy of natural parasitism in entomopathogenic nematodes, we explored the maintenance of fitness related traits including reproductive capacity, heat tolerance, virulence to insects and ‘tail standing’ (formerly called nictation) among laboratory-cultured lines derived from natural, randomly mating populations of S. carpocapsae. Laboratory cultured nematode …

Forage Soybean Yield And Quality Response To Water Use, David C. Nielsen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Forages could be used to diversify reduced and no-till dryland cropping systems from the traditional wheat (Triticum aestivum L.)-fallow system in the semiarid central Great Plains. Forages present an attractive alternative to grain and seed crops because of greater water use efficiency and less susceptibility to potentially devastating yield reductions due to severe water stress during critical growth stages. However, farmers need a simple tool to evaluate forage productivity under widely varying precipitation conditions. The objectives of this study were to (1) quantify the relationship between crop water use and dry matter (DM) yield for soybean (Glycine max …

Spatial Ramifications Of Crop Selection: Water Quality And Biomass Energy, M. P. Russelle, D. W. Kelley, A. S. Birr, D. C. Tiffany

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The use of GIS in concert with simple or complex simulation modeling provides an unparalleled way to generate new data and to help a variety of audiences understand spatial patterns of data. From improved understanding, policy incentives can be crafted to reduce adverse environmental impacts of agricultural production at lower costs than would be necessary otherwise. In this chapter, two case studies demonstrate how GIS and modeling can be used to understand how crop selection and soils interact to effect environmental outcomes across an agricultural landscape. We addressed the needs of two distinctly different audiences: (1) a public drinking water …

Nutritional Plane And Selenium Supply During Gestation Affect Yield And Nutrient Composition Of Colostrum And Milk In Primiparous Ewes, A. M. Meyer, J. J. Reed, T. L. Neville, J. F. Thorson, K. R. Maddock-Carlin, J. B. Taylor, L. P. Reynolds, D. A. Redmer, J. S. Luther, C. J. Hammer, K. A. Vonnahme, J. S. Caton

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The objectives were to investigate effects of nutritional plane and Se supply during gestation on yield and nutrient composition of colostrum and milk in first parity ewes. Rambouillet ewe lambs (n = 84, age = 240 ± 17 d, BW = 52.1 ± 6.2 kg) were allocated to 6 treatments in a 2 × 3 factorial array. Factors included Se [adequate Se (ASe, 11.5 µg/ kg of BW) or high Se (HSe, 77.0 µg/kg of BW)] initiated at breeding, and nutritional plane [60 (RES), 100 (CON), or 140% (HIH) of requirements] initiated at d 40 of gestation. Ewes were fed …