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Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 26 2025, Several Authors Jan 2025

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 26 2025, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Letter From The Dean, Jeff Edwards Jan 2025

Letter From The Dean, Jeff Edwards

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Phytochemical And Nutritional Composition Of Vernonia Amygdalina: Antioxidant, Antidiabetic Efficacy, And Hematological Benefits, Hassan A. Adewuyi, Messiah Atapia, Emmanuel Ochonung, Sakariyau A. Waheed, Adebimpe H. Oluwatoyin, Chizoba V. Obunadike, Adeleye A. Edema, Samad Hussein, Usman A. Mohammed, Kevin L. Sebastine, Tolulope O. Jaiyeola Jan 2025

Phytochemical And Nutritional Composition Of Vernonia Amygdalina: Antioxidant, Antidiabetic Efficacy, And Hematological Benefits, Hassan A. Adewuyi, Messiah Atapia, Emmanuel Ochonung, Sakariyau A. Waheed, Adebimpe H. Oluwatoyin, Chizoba V. Obunadike, Adeleye A. Edema, Samad Hussein, Usman A. Mohammed, Kevin L. Sebastine, Tolulope O. Jaiyeola

Chemistry & Biochemistry Faculty Publications

Vernonia amygdalina, a traditionally used medicinal plant, has been purported to possess health-promoting properties. The aim of this study is to investigate the phytochemical and nutritional composition of V. amygdalina, evaluate its antioxidant and antidiabetic efficacy, and assess its hematological benefits. Phytochemical screening, proximate analysis, antioxidant assays (DPPH, ABTS), antidiabetic evaluations in treptozotocin induced diabetic rats, and hematological assessments were conducted according to standard protocol. V. amygdalina exhibited a rich phytochemical profile, comprising flavonoids, phenolic acids, and terpenoids, alongside significant antioxidant activity (IC50 = 12.5 μg/mL) and complete source of protein. Proximate analysis revealed high protein (21.1%) and …


“Nice To See You”: Addressing Plant Awareness Disparity With Locally Specific Ecological And Cultural Plant Education, Cecilia Muecke Jan 2025

“Nice To See You”: Addressing Plant Awareness Disparity With Locally Specific Ecological And Cultural Plant Education, Cecilia Muecke

Pomona Senior Theses

Plants form the basis of life on Earth. However, due to the phenomenon of plant awareness disparity, a self-perpetuating cycle whereby plants are overlooked on an individual and global level ensues. Lack of plant awareness leads to a lessened emphasis on plants in sustainability and conservation as well as biology education, resulting in negative impacts on biodiversity and food security. The severity of plant awareness disparity is dependent on cultural context, and Indigenous and/or traditional societies are less likely to experience plant awareness disparity because of they are typically more connected to plants for their everyday needs. Native plants are …


Multi-Locas Gwas Mapping And Candidate Gene Analysis Of Anticancer Peptide Lunasin In Soybean (Glycine Max L. Merr), Rikki Locklear, Jennifer Kusumah, Layla Rashad, Felicia Lugaro, Sonia Viera, Nathan Kipyego, Faith Kipkosgei, Daisy Jerop, Shirley Jacquet, Mythy Addelmajid Kassem, Jiazheng Yuan, Elvira De Mejia, Rouf Mian Jan 2025

Multi-Locas Gwas Mapping And Candidate Gene Analysis Of Anticancer Peptide Lunasin In Soybean (Glycine Max L. Merr), Rikki Locklear, Jennifer Kusumah, Layla Rashad, Felicia Lugaro, Sonia Viera, Nathan Kipyego, Faith Kipkosgei, Daisy Jerop, Shirley Jacquet, Mythy Addelmajid Kassem, Jiazheng Yuan, Elvira De Mejia, Rouf Mian

Biological Sciences Faculty Publications

Soybean (Glycine max) peptide lunasin exhibits significant cancer-preventive, antioxidant, and hypocholesterolemic effects. This study aimed to identify quantitative trait nucleotides (QTNs) associated with lunasin content and to annotate the candidate genes in the soybean genome. The mapping panel of 144 accessions was gathered from the USDA Soybean Germplasm Collection, encompassing diverse geographical origins and genetic backgrounds, and was genotyped using SoySNP50K iSelect Beadchips. The lunasin content in soybean seeds was measured using the enzyme-linked immunosorbent assay (ELISA) method, with lipid-adjusted soybean flour prepared from seeds obtained from the Germplasm Resource Information Network (GRIN) of USDA-ARS in 2003 and …


Quantification Of Auxin-Cytokinin Response During Primary Root And Lateral Root Development In Common Bean, Gurdeep Singh Jan 2025

Quantification Of Auxin-Cytokinin Response During Primary Root And Lateral Root Development In Common Bean, Gurdeep Singh

Electronic Theses and Dissertations

Roots support water and nutrient uptake, anchorage, and soil interactions through continuous growth at the primary root tip (PRT). In common bean (Phaseolus vulgaris), new cells are produced in an open root apical meristem and subsequently elongate in the elongation zone, and together these processes drive root tip progression through the soil. Lateral roots (LRs) arise in the differentiation zone, where pairs of pericycle founder cells dedifferentiate, divide asymmetrically, and form lateral root primordia (LRP) that pass through defined developmental stages before emergence. Auxin and cytokinin (CK) are central regulators of both primary root growth and LR development, yet their …


Featured Faculty Mentor/Student Team, Kelly Loftin, Eva Berryhill Jan 2025

Featured Faculty Mentor/Student Team, Kelly Loftin, Eva Berryhill

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Exportin 1 Is The Central Hub In Antiviral Defensive Mobilization Of Nuclear Factors And The Target Of Counter-Defence By Tombusviruses, Biao Sun Dr. Jan 2025

Exportin 1 Is The Central Hub In Antiviral Defensive Mobilization Of Nuclear Factors And The Target Of Counter-Defence By Tombusviruses, Biao Sun Dr.

Theses and Dissertations--Plant Pathology

Positive-strand RNA viruses, such as coronaviruses and flaviviruses, are obligate parasites whose genomic RNAs function as mRNAs. These viruses infect a wide range of hosts-including plants, animals, and humans-resulting in significant economic losses and posing serious threats to public health. Due to the relatively simple structure of the genome of positive-strand RNA viruses, many host factors and physiological processes are co-opted to support virus infection and replication. In our previous studies, we identified numerous host factors and associated physiological processes involved in the replication of tombusviruses, a cytosolic-replicating model that includes tomato bushy stunt virus (TBSV) and carnation Italian ringspot …


Letter From The Faculty Editor, Beth Kegley Jan 2025

Letter From The Faculty Editor, Beth Kegley

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Quantifying Landscape Variability Of Dynamic Soil Properties In The James River Valley, Eastern South Dakota, Sage Reuter-Schmitt Jan 2025

Quantifying Landscape Variability Of Dynamic Soil Properties In The James River Valley, Eastern South Dakota, Sage Reuter-Schmitt

Electronic Theses and Dissertations

Dynamic soil properties (DSP) are a great tool for monitoring land-use change, and they are frequently used as soil health metrics. The NRCS and other soil researchers often use repeating, single-point or nested-point DSP studies to monitor and better understand the effects of land management decisions. While these studies capture the temporal variability of DSPs, they overlook the field-scale spatial variability of DSPs. Soil organic carbon (SOC) provides critical services to the ecosystem and other environments. These traits, as well as its susceptibility to management decisions, make SOC an important soil health indicator and DSP. Measuring SOC is often time …


Isolation And Characterization Of Rhizobia Bacteria From Midwest Soils, Jr Loya Jan 2025

Isolation And Characterization Of Rhizobia Bacteria From Midwest Soils, Jr Loya

Electronic Theses and Dissertations

Symbiotic bacteria, known as rhizobia, can colonize the roots of legume plants and then convert atmospheric nitrogen into a usable form by the host through the process called biological nitrogen fixation (BNF). Many of these bacteria produce other plant-growth-promoting benefits, such as nutrient acquisition, phytohormone production, and the modification of plant stress responses. Agricultural inoculants made from these bacteria have helped reduce the need for man-made nitrogenous fertilizers, known causes of pollution and eutrophication. There are additional benefits in developing bacteria inoculants local to regional soils. The ability to survive and compete in Midwest soil and climates present an opportunity …


Npr1 As A Temperature-Sensitive Regulator Of Pipecolic Acid-Mediated Plant Systemic Immunity, Spencer Tout Jan 2025

Npr1 As A Temperature-Sensitive Regulator Of Pipecolic Acid-Mediated Plant Systemic Immunity, Spencer Tout

Theses and Dissertations (Comprehensive)

Healthy plant development requires balancing both abiotic (e.g. climate) and biotic (e.g. pathogen) challenges, which intercept important signaling pathways in plants. For example, climate change-associated elevated temperature can negatively modulate various aspects of plant immunity, including the production of central immune signals salicylic acid (SA) and N-hydroxypipecolic acid (NHP). Since SA and NHP signaling both require the master immune co-activator protein NON-EXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1), it is important to investigate NPR1 as a potentially temperature-regulated molecular hub. Although the NPR1 gene is not transcriptionally downregulated at elevated temperatures (28° C vs. 23° C), little is known about …


Generational Exposures To Cerium Oxide Nanoparticles And Perfluorooctanesulfonic Acid Affect The Performance Of Daughter Plants, Preston Clubb Jan 2025

Generational Exposures To Cerium Oxide Nanoparticles And Perfluorooctanesulfonic Acid Affect The Performance Of Daughter Plants, Preston Clubb

Graduate Theses/Dissertations

The effects of parental stress on the performance of next generation exposed to another contaminant were investigated. Wheat was exposed to cerium oxide nanoparticles (CeO2-NPs) in first and second generations and exposed to perfluorooctanesulfonic acid (PFOS) in the third generation. Phenotypic or metabolic responses were assessed at 21-day (short-term exposure) or 90-day (long-term exposure) exposure periods. Biomass production, chlorophyll content, enzyme activity, and membrane damage were measured at short-term exposure, while elemental and PFOS concentrations, and grain metabolites were analyzed in long-term exposure. Results showed that continued exposures to CeO2-NPs and PFOS enhanced chlorophyll content but reduced concentrations of important …


An Economic And Production Analysis Of Seaweed Farms In California And The Pacific Northwest, Brian Donovan Jan 2025

An Economic And Production Analysis Of Seaweed Farms In California And The Pacific Northwest, Brian Donovan

Cal Poly Humboldt theses and projects

Since the early 2010s, the seaweed aquaculture industry has steadily expanded in the United States, with states like Alaska, Maine, Hawaii, and Connecticut leading the way in the development of this emerging sector. However, information gaps remain concerning certain aspects of the domestic seaweed aquaculture industry. The purpose of this study was to examine the status of the seaweed aquaculture industry along the West Coast of the United States; specifically in the states of California, Oregon, and Washington. Seaweed farmers in these states were invited to voluntarily complete surveys asking important production, economic, and commercial questions regarding their operations. Data …


Raman Spectroscopy For Rapid Quantification Of Macronutrients In Heirloom Beans, K. Chandler Williams Jan 2025

Raman Spectroscopy For Rapid Quantification Of Macronutrients In Heirloom Beans, K. Chandler Williams

Graduate Theses, Dissertations, and Problem Reports (ETD)

Quantification of macronutrients in food requires a large investment in facilities and instrumentation as well as ongoing expenses of labor, reagents, and hazardous waste disposal. These costs pose a significant barrier to determining macronutrient quantities in the thousands of uncharacterized landrace and heirloom crops and discourage traditional crop breeding projects from ensuring that their selected crops maintain desirable macronutrient profiles in addition to target agronomic traits. Our objective was to test whether a hand-held Raman spectrometer—a technology which has found use in plant disease diagnostics but remains largely overlooked in nutrient quantification-- could provide a feasible means for quantifying the …


The Aussie Oddballs: Identifying The Existence Of Censer And Trample Burr Dispersal In Australian Bush Tomatoes, Abigail Motter Jan 2025

The Aussie Oddballs: Identifying The Existence Of Censer And Trample Burr Dispersal In Australian Bush Tomatoes, Abigail Motter

Honors Theses

Australian bush tomatoes in northern Western Australia likely have seed dispersal mechanisms that depend on processes outside of frugivory, which is unusual for this genus. Solanum tudununggae seeds are wind dispersed by the censer mechanism, meaning that seeds fall from holes in the top of the fruit when the branches move. In another species, piles of abscised and burr-like fruits have been found strewn around the bases of S. ossicruentum, possibly due to the extinction of animals that may have been a means of dispersal via epizoochory. In this study, we attempt to provide support for the theorized dispersal …


Effects Of Round-Up On The Environment, Sandra J. Marcu Jan 2025

Effects Of Round-Up On The Environment, Sandra J. Marcu

Journal of Earth and Life Science

Many people around the world have used and still currently use Roundup but are unaware of the effects it has on the environment. Roundup is a spray on application weedkiller that is widely used around the world today both residentially and commercially. It enables its user to grow a garden or a field of crops with a no-tilling approach to eliminate weeds. It is a well-known and popular choice for killing weeds that has been around since the mid 1970’s (Oca, 2017). John Franz, a Monsanto scientist discovered that glyphosate (main ingredient in Roundup) was an herbicide or weedkiller, and …


A Mystery Of Microecology: Revealing The Ecological Relationship Between Allorhizobium Vitis And Missouri Native Grape Species, Hunter White Jan 2025

A Mystery Of Microecology: Revealing The Ecological Relationship Between Allorhizobium Vitis And Missouri Native Grape Species, Hunter White

Graduate Theses/Dissertations

Crown gall disease can be very detrimental to grape and wine producers around the world by reducing vine vigor and yield. It is caused by the bacteria Allorhizobium vitis (A. Vitis) carrying a tumor inducing (Ti) plasmid. The A. vitis bacteria have been found in soil and cane tissues in vineyards around the world, and infect all cultivated grapes. However, there have been no reports of crown gall on native wild grapevines, and very little is known about the ecology of A. vitis in native ecosystems. I have sampled cambium tissues from three native wild grape species of …


The Effects Of Calcium Availability On Growth And Calcium Content Of Hydroponic Daikon Radish Microgreens, Cory Michael Reichmuth Jan 2025

The Effects Of Calcium Availability On Growth And Calcium Content Of Hydroponic Daikon Radish Microgreens, Cory Michael Reichmuth

Graduate Theses/Dissertations

Microgreens are young, edible seedlings harvested upon emergence of the first true leaf. Their popularity is growing as a nutritionally dense food source that can be grown quickly in space-efficient systems. However, little is known about optimal cultivation practices and the effects of fertilization on growth and nutrition of microgreens. This study investigates the role of calcium (Ca) fertilization in optimizing the growth, yield, and nutrient content of daikon radish (Raphanus sativus) microgreens. A hydroponic system was designed to consistently deliver nutrient solution to microgreens grown in a closed-environment chamber. This study tested different Ca fertilization rates of 0, 5, …


Inoculating Soybean Plants At Different Growth Stages: Selecting Bradyrhizobium, Sinorhizobium, Or Both, Matthew R. Knoll Jan 2025

Inoculating Soybean Plants At Different Growth Stages: Selecting Bradyrhizobium, Sinorhizobium, Or Both, Matthew R. Knoll

Graduate Theses/Dissertations

Soybean plants rely on nitrogen for growth, however atmospheric nitrogen (N2) is not a usable form and needs to be converted into different forms that are available to the plant. Nitrogen fixing bacteria such as rhizobia form symbiotic relationships with Soybeans in exchange for shelter and excess plant sugars. As a result, the plant can get the nitrogen it needs. There are four main genera of rhizobia that can form root nodules on Soybean including Bradyrhizobium, Sinorhizobium, Mesorhizobium, and Rhizobium. At this point, the effect that age of the host plant has on the selection of …


Restoration Of Giant River Cane (Arundinaria Gigantea) In Southwest Missouri, Lyndsey D. Rightnowar Jan 2025

Restoration Of Giant River Cane (Arundinaria Gigantea) In Southwest Missouri, Lyndsey D. Rightnowar

Graduate Theses/Dissertations

Giant river cane (Arundinaria gigantea), a native bamboo species, once dominated riparian landscapes across the southeastern United States but has declined to approximately 2% of its historic range. This study evaluated restoration techniques for giant cane in southwest Missouri by examining the influence of rhizome source (putative genotype), planting type, and restoration site on restoration success. I compared two planting types: greenhouse-to-field (G2F) transplanting, where rhizomes were first propagated in the greenhouse before planting, and field-to-field (F2F) transplanting, where rhizomes were directly transplanted from source canebrakes to restoration sites. Rhizomes were collected from four existing canebrakes in Taney …


Physiological And Genetic Studies Of Hydrotropism In The Maize Primary Root, Muyu Gu Jan 2025

Physiological And Genetic Studies Of Hydrotropism In The Maize Primary Root, Muyu Gu

Electronic Theses and Dissertations

No abstract provided.


Testing The Effect Of Source And Environment On Native Seed Germination To Inform Seed-Based Restoration Of Grasslands, Brandon Clark Jan 2025

Testing The Effect Of Source And Environment On Native Seed Germination To Inform Seed-Based Restoration Of Grasslands, Brandon Clark

Electronic Theses and Dissertations

Grasslands are one of the most endangered ecosystems on the planet, and they provide a variety of important ecosystem services that people rely on. Losses of grasslands can be mitigated through restoration. Seed-based restoration is a regularly used practice due to its practicality and cost-effectiveness. Planting non-local seed that may not be suitable for its destination environment can have consequences in plant establishment and subsequent restoration success. This experiment tested the response (final germination and germination rate) of seed from 11 perennial tallgrass species sourced from 3 different locations to different germination temperature conditions. Seeds from three sources of each …


Leaf-Based Varietal Categorization Of Sweetpotato (Ipomoea Batatas L. Lam.), A Potentially Healthful Vegetable, Using Image Processing And K-Means Clustering, Shahidul Islam, Md Towfiqur Rahman, Md Hamidul Rahman, Abdul Momin Jan 2025

Leaf-Based Varietal Categorization Of Sweetpotato (Ipomoea Batatas L. Lam.), A Potentially Healthful Vegetable, Using Image Processing And K-Means Clustering, Shahidul Islam, Md Towfiqur Rahman, Md Hamidul Rahman, Abdul Momin

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Sweetpotato (Ipomoea batatas Lam) leaves contain higher concentrations of phenolic compounds, flavonoids, and carotenoids that are remarkable in health promotion. However, the nutrient content in sweetpotato leaves varies from variety to variety, and leaf shape and color are the key identifying factors for the varietal classification of sweetpotatoes. So, detecting sweetpotato leaves is essential for the in-situ identification of sweetpotato varieties and for developing intelligent agricultural systems. This study aimed to create a leaf-shape-based varietal classification technique for sweetpotato using image processing techniques coupled with a K-means clustering algorithm. 38 leaf images (RGB) of two sweetpotato cultivars were collected …


Bridging Prairie Restoration And Regenerative Agriculture With Compost Tea / The Rocket Incident: Post-Fire Soil Microbe Recovery On The Eastern Washington University Restoration Site, April Hersey Jan 2025

Bridging Prairie Restoration And Regenerative Agriculture With Compost Tea / The Rocket Incident: Post-Fire Soil Microbe Recovery On The Eastern Washington University Restoration Site, April Hersey

EWU Masters Thesis Collection

Inland Pacific Northwest prairie is an endangered ecosystem that has been reduced by conversion to agriculture. There is growing interest in the role of soil microorganisms in prairie ecosystems, which may be crucial for restoration of the plant community through interspecies interactions within the rhizosphere. One method for improving soil microorganism community composition is compost tea: enriched liquid compost extract. Compost tea is used in regenerative agriculture to improve crop yields and protect against pathogens. While research has supported compost tea application in regenerative agriculture, it has not been widely studied in the context of ecosystem restoration. After beginning a …


Foraging Opportunists: Encouraging Reconnection With The Land, Madelyne Miller, Sylvia Blodorn, Aine Fannon, Ada Scott, Luke Pruitt Jan 2025

Foraging Opportunists: Encouraging Reconnection With The Land, Madelyne Miller, Sylvia Blodorn, Aine Fannon, Ada Scott, Luke Pruitt

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Contextualizing the history of opportunist and native plant species opens options for land management and reconnection to cultural land practices. By recognizing that opportunist species often spread as a result of relocation for practical usage, we determine that reintroducing these uses can lead to an escalation in harvesting these plants. Uses of opportunist plants are diverse and include food sources, artistic practices, and medicine. Foraging also leads to improved human-land-connectedness as well as a deeper connection to ancestral and cultural traditions, which has been found to improve mental health. Focusing on this method of land management will limit the need …


Skeleton Weed In Western Australia: 2025 Management Guide, Department Of Primary Industries And Regional Development, Western Australia Jan 2025

Skeleton Weed In Western Australia: 2025 Management Guide, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity bulletins

Skeleton weed can reduce crop yields by competing for moisture and nutrients (mainly nitrogen).

This guide helps you manage and eradicate skeleton weed infestations on your property, and helps to stop the spread across WA.

Without the coordinated program aimed at controlling the spread, skeleton weed would now be much more abundant and widely established throughout cereal growing areas.

If you find skeleton weed on your property, we can help you get rid of it.

Skeleton weed is unpredictable and can spread over long distances due to wind dispersal, but once identified it can be managed effectively and eradicated


Skeleton Weed In Western Australia: Control Program 2024–2025, Department Of Primary Industries And Regional Development, Western Australia Jan 2025

Skeleton Weed In Western Australia: Control Program 2024–2025, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity bulletins

Key program changes

Winter eradication program

New herbicide recommendations for control in crops, legume based annual pastures, plantations and glyphosate tolerant canola.

  • The minimum size of ‘squares’ (infested areas) has been reduced from 40 m x 40 m (0.16 ha) to 20 m x 20 m (0.04 ha) to help reduce the winter treatment area.
  • Landholders will need to consult with DPIRD/LAG officers to assess the degree of infestation before working or cultivating through squares in Code 1 paddocks.
  • Clopyralid (LontrelTM) herbicide will no longer be provided for treatment on heavily infested paddocks. Landholders will still be required to undertake …


Characterizing Agroecosystem Effects Of Industrial Hemp On A Rotational Row Cropping System And Microbial Communities, Kent D. Pham Jan 2025

Characterizing Agroecosystem Effects Of Industrial Hemp On A Rotational Row Cropping System And Microbial Communities, Kent D. Pham

Theses and Dissertations--Plant and Soil Sciences

The implementation of crop rotation on a farm can have many benefits for agroecosystem health and production. This practice can potentially reduce nutrient amendments, disrupt pest and disease cycles, and increase crop yields. In Kentucky, one common rotation consists of either corn or soybean grown the first year, followed by the other crop, repeated every two years. Industrial hemp, grown for fiber or grain, is an emerging crop that has shown promise as a rotation alternative, although not much is known about its effects on soil health, agroecosystem functioning, and microbial communities. To better characterize hemp’s role within American agriculture, …


Factors Impacting Germination, Emergence, And Establishment Of Direct Seeded And Transplanted Hemp (Cannabis Sativa L.), Paul Cockson Dr. Jan 2025

Factors Impacting Germination, Emergence, And Establishment Of Direct Seeded And Transplanted Hemp (Cannabis Sativa L.), Paul Cockson Dr.

Theses and Dissertations--Plant and Soil Sciences

Industrial hemp (IH) establishment has many challenges including: poor emergence, low stand establishment, and variable plant development in direct seeded hemp. In floral hemp production, quality transplant production is a challenge with seedlings often becoming root bound and etiolated. In both direct seeded and transplant hemp, the relationship between root vigor and vegetative growth has not been studied and may prove insightful for managing emergence and establishment challenges. The goals of this study were three-fold: 1) to measure and describe root system architecture (RSA) and root phenotypes observed in selected IH cultivars especially in regard to identifying agronomically important RSA …