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Phenolic Content, Antibacterial, Antioxidant, And Toxicological Investigations Oferodium Guttatum (Geraniaceae) Collected From The Northeast Of Morocco, Hanae Naceiri Mrabti, Latifa Doudach, Mohamed Reda Kachmar, Abdelaziz Ed Dra, Zineb Khali, Nidal Naceiri Mrabti, Kaoutar Benrahou Benrahou, Khouloud Harraqui, Gökhan Zengi̇n, Abdelhakim Bouyahya, Moulay El Abbes Faouzi

Turkish Journal of Botany

Erodium genus contains several medicinal traditionally used and pharmacologically explored. However, Erodium guttatum has not been well valorized. Therefore, the aim of the present study was to evaluate the antibacterial and antioxidant activities of E. guttatum extracts in addition to their toxicity. To achieve the objectives of this study, methanol and aqueous extracts of E. guttatum were prepared. Then, antibacterial activity was evaluated against Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 43816, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and Listeria monocytogenes ATCC 13932 by disc diffusion and broth microdilution methods. The antioxidant activity was …

Chloroplast Genome Evolution In The Klebsormidiophyceae And Streptofilum, Sarah Glass

Theses and Dissertations

The Klebsormidiophyceae are a class of green microalgae found globally in both freshwater and terrestrial habitats. Morphology-based classification schemes of this class have been shown to be insufficient, particularly due to the simple morphology of these algae, the tendency of morphology to vary in culture versus in field conditions, and rampant morphological homoplasy. Molecular studies revealing cryptic diversity have renewed interest in this group. We sequenced the complete chloroplast genomes of taxa in the Klebsormidiophyceae representing the known taxonomic breadth of this class, as well as the chloroplast genomes of three strains of Streptofilum, a recently discovered algal lineage …

Restoration Through Regeneration: An Analysis Of Agriculture In The United States, Rebecca Norine Graham

Capstone Showcase

The industrialization of agriculture in the mid-twentieth century has revolutionized global food systems, with enough food produced annually to sustain 10 billion people on a planet of only 7.8 billion people. Such a high-yielding agricultural system comes at a great cost to the Earth’s ecosystem, as industrialized farming has taken on factory-like production methods. Particularly harmful is the livestock sector, with ruminating animals such as cattle contributing to rising global temperatures and environmental degradation. The environmental destruction caused by industrialized animal agriculture can be halted and ultimately reversed through regenerative agriculture. Regenerative agriculture is a restorative farming practice that promotes …

Efficacy And Mechanism Of Acs811 In Tomato Immune Responses, Layne Ellen Harris

Theses and Dissertations--Plant and Soil Sciences

Biologicals are increasingly becoming an important part of sustainable integrated pest management in agricultural crop production and encompass a wide variety of products with varying degrees of efficacy and available research data. As biologicals become more integrated into commercial production systems, it is critical to rigorously evaluate biological product efficacy and mode(s) of action in crops. The better that biologicals are understood in terms of effective application, integration into commercial programs, and their specific mechanisms in crop growth and protection, then the greater the opportunity for increasing yields and food security.

ACS811 is a microbial fermentation-based biological product from Alltech …

Concomitant Accumulations Of Ions, Osmoprotectants And Antioxidant System-Relatedsubstances Provide Salt Tolerance Capability To Succulent Extreme-Halophyte Scorzonera Hieraciifolia, Cansu Altuntaş, Rabi̇ye Terzi̇

Turkish Journal of Botany

Halophytes adapting to live in salinized areas can activate some tolerance mechanism through signal compounds to cope with salinity. However, the role of co-activity of signal compounds in salt tolerance of halophytes has not yet fully understood. We researched the changes in signal compounds involved in the salt tolerance mechanism, including inorganic ions, osmoprotectants and substances related to the antioxidant system in

Biofortification Of Sodium Selenate Improves Dietary Mineral Contents And Antioxidant Capacity Of Culinary Herb Microgreens, Rachel G. Newman, Youyoun Moon, Carl E. Sams, Janet C. Tou, Nicole L. Waterland

Faculty & Staff Scholarship

Selenium biofortification of plants has been suggested as a method of enhancing dietary seleniumintake to prevent deficiency and chronic disease in humans, while avoiding toxic levels of intake. Popular herbs such as basil (Ocimum basilicum L.), cilantro (Coriandrum sativum L.), and scallions (Allium fistulosum L.) present an opportunity for biofortification as these plants are used for added flavors to meals and are available as microgreens, young plants with increasing popularity in the consumer marketplace. In this study, basil, cilantro, and scallion microgreens were biofortified with sodium selenate under hydroponic conditions at various selenium concentrations to investigate the effects on yield, …

Chaetocin Enhances Callus Induction By Decreasing The Expression Of Major Leaf Polarity Genes In Nicotiana Tabacum, Ayyub Ebrahimi, Nagi̇han Özsoy, Saadet Deni̇z Gürle, Baki̇ Yaman, Şule Ari

Turkish Journal of Botany

Using chemicals known as inhibitors of chromatin remodeling enzymes is a useful approach in understanding the regulation machinery that contributes to the maintenance of or conversion to a pluri- and totipotent states of the cells in multicellular organisms. They can serve as tools to reveal the molecular and cellular mechanisms required for reprogramming. A deeper understanding of reprogramming pathways that drive the cell into a dedifferentiated state might help us enhance plant micropropagation and green biotechnology applications. Chaetocin is one of the histone methyltransferase inhibitors known as key drivers in several cellular processes. It was shown that chaetocin enhanced the …

Examining The Utility Of Dna Barcodes For The Identification Of Tallgrass Prairie Flora, Sarah A. Herzog, Maribeth Latvis

Natural Resource Management Faculty Publications

Premise
The tallgrass prairies of North America are one of the most threatened ecosystems in the world, making efficient species identification essential for understanding and managing diversity. Here, we assess DNA barcoding with high‐throughput sequencing as a method for rapid plant species identification.
Methods
Using herbarium collections representing the tallgrass prairie flora of Oak Lake Field Station, South Dakota, USA, we amplified and examined four common nuclear and plastid barcode regions (ITS, matK, psbA‐trnH, and rbcL), individually and in combination, to test their success in identifying samples to family, genus, and species levels using BLAST searches of …

Soil Response Of Helicopter Liming In The Monongahela National Forest, Jarrett Douglas Fowler

Graduate Theses, Dissertations, and Problem Reports (ETD)

Soils in the Monongahela National Forest (MNF) are acidic due to sandstone parent material, acid deposition, uptake of base cations by vegetation, and release of organic acids by organic matter (OM) decomposition. Increases in soil acidity have caused declines in forest health and changed species composition and nutrient status. Liming can neutralize soil acidity, but no large-scale liming projects have been done on acid forest soils in the USA. In anticipation of acquiring funding for a proposed liming project in the MNF, in 2007 and 2009 10 sites were selected to sample and analyze soils before lime was applied. In …

Sterol Biosynthesis In Four Green Algae: A Bioinformatic Analysis Of The Ergosterol Versus Phytosterol Decision Point, Adam Voshall, Nakeirah T.M. Christie, Suzanne L. Rose, Maya Khasin, James L. Van Etten, Jennifer E. Markham, Wayne R. Riekhof, Kenneth Nickerson

School of Biological Sciences: Faculty Publications

Animals and fungi produce cholesterol and ergosterol, respectively, while plants produce the phytosterols stigmasterol, campesterol, and bsitosterol in various combinations. The recent sequencing of many algal genomes allows the detailed reconstruction of the sterol metabolic pathways. Here, we characterized sterol synthesis in two sequenced Chlorella spp., the free-living C. sorokiniana, and symbiotic C. variabilis NC64A. Chlamydomonas reinhardtii was included as an internal control and Coccomyxa subellipsoidea as a plant-like outlier. We found that ergosterol was the major sterol produced by Chlorella spp. and C. reinhardtii, while C. subellipsoidea produced the three phytosterols found in plants. In silico analysis …

Optical Seed Sorter-Based Selection Lowers Deoxynivalenol Accumulation In Soft Red Winter Wheat, William Jesse Carmack

Theses and Dissertations--Plant and Soil Sciences

Fusarium head blight (FHB) of wheat (Triticum aestivum L.) results in discolored grain contaminated with deoxynivalenol (DON). DON accumulation, an indicator of FHB resistance, can be quantified and used as the basis for direct phenotypic selection, but testing is expensive. The primary objective of this study was to evaluate an optical seed sorter as an alternative to DON testing for FHB resistance breeding. Three hundred F4 derived soft red winter wheat (SRWW) breeding lines were grown in an inoculated FHB nursery over several years in Lexington, KY. Grain from each breeding line was sorted using an optical seed sorter calibrated …

Incorporation Of Summer Annual Mixtures Into Grazing Systems In Kentucky, Kelly Marie Mercier

Theses and Dissertations--Plant and Soil Sciences

Utilizing summer annual grass-legume forage mixtures has the potential to improve forage yield and nutritive characteristics, and/or animal performance during times when cool-season pasture growth is limited by high temperatures. Legumes can utilize atmospheric nitrogen, which can increase crude protein and forage digestibility in mixtures. As nitrogen application generally improves both the yield and nutritive characteristics of summer annual forages, but can have a negative effect on legume competitiveness, nitrogen fertilizer recommendations for legume-containing summer annual mixtures are not well established.

Two experiments were conducted to determine the feasibility of utilizing summer annual mixtures in Kentucky, USA. The first experiment …

Genomic Selection Strategies To Predict Grain Yield And Disease Resistance Traits In A Wheat Breeding Program, Virginia Laura Verges

Theses and Dissertations--Plant and Soil Sciences

Genomic selection (GS) is a form of marker-assisted selection (MAS) that simultaneously estimates all locus, haplotype or marker effects across the entire genome to calculate genomic estimated breeding values (GEBVs). Since its inception, it has had the attention of breeders keen on finding tools to accelerate genetic gain and reduce phenotyping costs in the breeding program. A first objective of this study was to evaluate strategies to design the training population (TP) and validating population (VP) to estimate GEBVs for grain yield and agronomic traits for wheat breeding lines. Our results demonstrate that, despite the small family size, an approach …

New Additions To Turkish Mycota From Ankara, Balıkesir, And Kütahya Provinces, Deni̇z Altuntaş, Fuat Bozok, Hatira Taşkin, Şanli Kabaktepe, Hakan Alli, Ilgaz Akata

Turkish Journal of Botany

The current research was conducted on the fungal materials collected from Ankara, Balıkesir, and Kütahya provinces between 2015 and 2019. As a result of the field and laboratory studies, Conocybe dentatomarginata, Entoloma lampropus, Lactarius lilacinus, Otidea nannfeldtii and Sarcopeziza sicula are reported for the first time from Turkey based on macro- and micromorphological studies and internal transcribed spacer (ITS) rDNA-based molecular phylogeny. Descriptions of new records are provided together with photos showing their macro and micro-morphology.

Black Soldier Fly Larval Meal In Feed Enhances Growth Performance, Carcass Yield And Meat Quality Of Finishing Pigs, S. Y. Chia, C. M. Tanga, I. M. Osuga, A. O. Alaru, D. M. Mwangi, M. Githinji, T. Dubois, S. Ekesi, J. J.A. Van Loon, M. Dicke

All Peer-Reviewed Publications

Using black soldier fly (BSF, Hermetia illucens) larvae as a novel protein source substituting fishmeal (FM) in animal feeds is globally gaining momentum. BSF can be reared on agro-industrial residues. However, incorporating BSF larval meal (BSFLM) into finisher pig diets has received inadequate attention. This study evaluated the effects of replacing dietary FM with BSFLM on growth, carcass traits and meat quality of finisher pigs. A control diet (including FM: 0% BSFLM) was compared with four dietary levels of replacement of FM with BSFLM at 25, 50, 75 or 100%. Forty hybrid pigs (crossbreeds of purebred Large White and Landrace) …

The Mechanisms And Consequences Of Shrub Encroachment On The Virginia Barrier Islands, Lauren K. Wood

Theses and Dissertations

Shrub encroachment is a global phenomenon driven by direct and indirect anthropogenic influence which alters plant communities and ecosystem function. Many studies have investigated drivers and consequences of woody plant establishment, but mesic landscapes are underrepresented in the literature. My objective was to assess the mechanisms of Morella cerifera encroachment into coastal mesic grassland, the potential for self-reinforcement, and consequences on community composition, nutrients, and landscape productivity. I studied temperature and water microclimate modification by Morella cerifera presence and removal to understand ecosystem engineering and community composition changes. Additionally, I examined the influence of shrubs on surrounding grassland species traits …

The Importance Of Store Hygiene For Reducing Post-Harvest Losses In Smallholder Farmers’ Stores: Evidence From A Maize-Based Farming System In Kenya, Kobia J. Makinya, John M. Wagacha, Judith A. Odhiambo, Paddy Likhayo, Kukom Edoh-Ognakossan, Tadele Tefera, Adebayo Abass, Christopher M. Mutungi

All Peer-Reviewed Publications

Knowledge of the role of hygiene in reducing food loss in farm stores is limited among extensionists, researchers, and farmers. Store hygiene practices during post-harvest handling and storage of maize were assessed using a cross-sectional survey of 342 farmers, with regular follow-up of 40 farmers’ stores over seven months to measure losses caused by insects, and to score the hygiene levels using a standard checklist. Fractional Response Model was used to evaluate the associations between hygiene practices and the losses. Farmers stored their produce in sacks (98.2%) kept in outside granaries (60.1%), or rooms in dwelling houses (39.9%). Co-storage with …

Elucidation Of Multi-Origin Inula Helenium L. For Antimicrobial Activity And Heavy Metal Profiling As An Exemplar For The Use And Regulation Of Plants As Antibiotics., Ciara Ruth Kenny

Theses

An impending post-antibiotic era looms, thus new sources of therapeutic chemical entities are crucial to sustain effective antimicrobial chemotherapy worldwide. Plant natural products are a source of accessible, structurally diverse compounds that provide therapeutic potential. The pharmacological applications of plants in medicine can be guided by the attestation of traditional use or the application of this knowledge to uncover new leads in the drug discovery process. The range of modified in vitro methods commonly used to investigate preclinical antimicrobial efficacy of plant-derived natural products, and the associated limitations and challenges in the provision of new antimicrobial drugs from plants is …

Earthworm Differences Among Mound Positions In Native Prairies In The Ozark Highlands, Carlee D. Hensley, Kristofor R. Brye, Mary C. Savin, Lisa S. Wood, Edward E. Gbur

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Mounded, tallgrass prairie ecosystems are a unique, endangered ecosystem due to severe disturbance for agricultural production and/or urban expansion. Limited research exists on earthworm (Lumbricus terrestris) dynamics in mounded, tallgrass prairies, particularly in the Ozark Highlands region of northwestern Arkansas. The objectives of this study were to evaluate the effects of soil moisture regime (SMR), landscape position (LP), and sample date on earthworm densities in the top 20 cm of soil in two mounded, native prairies in the Ozark Highlands region of northwestern Arkansas. Juvenile and total earthworm densities were five times greater (P < .05) in the udic- and aquic-inter-mound combinations than any other SMR-LP combination in October 2018 and May 2019, respectively. Adult earthworm densities were two times greater (P < .05) in the aquic-inter-mound at Searles Prairie than any other SMR-LP combination in either mounded prairie. Juvenile earthworm densities were three times greater (P < .05) in May 2019 than in May 2018 at Chesney Prairie, but adult and total earthworm density did not change over the same period of time. Combined across all data, juvenile and total earthworm densities were positively correlated (P …