Digital Commons Network^™

Relationships Of Intercropped Maize, Stem Borer Damage To Maize Yield And Land-Use Efficiency In The Humid Forest Of Cameroon, A. Chabi-Olaye, C. Nolte, F. Schulthess, C. Borgemeister

All Peer-Reviewed Publications

Stem borers are the most important maize pests in the humid forest zone of Cameroon. Field trials were conducted in the long and short rainy seasons of 2002 and 2003 to assess the level of damage and yield reductions caused by stem borers in monocropped maize and in maize intercropped with non-host plants such as cassava, cowpea and soybean. The intercrops were planted in two spatial arrangements, i.e. alternating hills or alternating rows. All intercrops and the maize monocrop were grown with and without insecticide treatment for assessment of maize yield loss due to borer attacks. The land-use efficiency of …

Characterization Of Waxy Grain Sorghum Lines In Relation To Granule-Bound Starch Synthase, J. F. Pedersen, S. R. Bean, R. A. Graybosch, S. H. Park, M. Tilley

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The waxy phenotype, associated with endosperm containing little or no amylose, has been recognized in sorghum (Sorghum bicolor L. Moench) since 1933. Although variants of the waxy gene are well characterized in other cereals, the waxy trait has been assumed to be controlled by a single allele, wx, in sorghum. Recent improvements in technologies encourage re-examination of the waxy sorghums. The objectives of this research were therefore to identify and characterize sorghum lines with differing waxy alleles and to describe the actions of those alleles in crosses. Grain of eight waxy sorghum lines (BTxARG1, BTx630, Tx2907, B.9307, 94C274, …

Biotinylation Of K12 In Histone H4 Decreases In Response To Dna Double-Strand Breaks In Human Jar Choriocarcinoma Cells1,2, Nagarama Kothapalli, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We tested the hypothesis that biotinylation of K12 in histone H4 plays a role in the cellular response to double-strand breaks (DSB) of DNA in human cells. DSB were caused by treating choriocarcinoma JAr cells with etoposide. Biotinylation of K12 in histone H4 decreased by 50% as early as 10–20 min after initiation of treatment with etoposide. Biotinylation returned to initial levels 30–40 min after the addition of etoposide to the medium. Temporal patterns of K12-biotinylation were similar for human lymphoma cells. Phosphorylation of S14 of histone H2B and poly(ADP-ribosylation) of glutamate residues on histone H2A are known markers of …

Nebline, September 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Now is a Good Time to Start a 4-H Club!
Spotlight on a New 4-H Club: Happy Hearts
4-H Kick Off, Sept. 20
Harvesting and Storing Apples
Garden Plants with Silver Foliage
Holiday Plants, Will They Bloom Again?
Swarming Midges near Capital Beach
Hands-On Termite Applicator Training, Sept. 28 & 20
Itch Mites Increasing in Oak Galls: Be Prepared for a Possible Itch Mite Outbreak
Pollution Prevention Participation
Harvest Safety Reminders
Women in Agriculture Conference Sept. 15–16
Prepare Bins Before Harvest to Maintain Grain Quality and Value
Scrap Tire Collection
Resting Pastures — the Key to More Productive Pastures
Upcoming …

Forage News [2005-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Scott County to Host Fall Grazing School
• Now is the Time to Test
• Economics of Grazing Stocker Cattle as a Sustainable Alternative to Row Crops
• Roundup Ready Alfalfa
• Novel Endophyte Tall Fescue
• Is It Too Late to Stockpile This Year?
• KFGC Awards
• KFGC Executive Committee to Meet September 6
• Wisconsin Grazing Dairy Farms
• Upcoming Events

Effects Of Commercial Diazinon And Imidacloprid On Microbial Urease Activity In Soil And Sod, C. W. Ingram, Mark S. Coyne, David W. Williams

Plant and Soil Sciences Faculty Publications

Diazinon [O,O-diethyl O-2-isopropyl-6-methyl(pyrimidine-4-yl) phosphorothioate] and imidacloprid [1-(1-[6-chloro-3-pyridinyl]methyl)-N-nitro-2-imidazolidinimine] are applied to lawns for insect control simultaneously with nitrogenous fertilizers such as urea, but their potential effect on urease activity and nitrogen availability in turfgrass management has not been evaluated. Urease activity in enzyme assays, washed cell assays, and soil slurries was examined as a function of insecticide concentration. Intact cores from field sites were used to assess the effect of insecticide application on urease activity in creeping bentgrass (Agrostis palustris Huds.) and bluegrass (Poa pratensis L.) sod. Bacterial urease fromBacillus pasteurii and plant …

Assessing The Health Of Fringing Salt Marshes Along The Fore River And Its Tributaries, Pamela A. Morgan, Lucas Curci, Cayce Dalton, Jeremy Miller

Environmental Studies Faculty Publications

Casco Bay has experienced two significant oil spills in recent history - the Tamano Tanker spill in 1972 and the Julie N spill in 1996. In addition, the Fore River and its tributaries have experienced numerous small spills over the years, including the August 2002 fuel oil spill (2,900 gallons) and the April 2003 jet fuel spill (6,000 gallons). The impacts of these spills on the fringing salt marshes that line the edges of the Fore River and its tributaries are not well understood. Are these salt marshes resilient enough to withstand these impacts and still act as healthy marshes …

Effects Of Summer Grazing Strategies On Organic Reserves And Root Characteristics Of Big Bluestem, E. M. Mousel, Walter H. Schacht, C. W. Zanner, Lowell E. Moser

Department of Agronomy and Horticulture: Faculty Publications

Quantifying root structure response to multiple defoliation events in a grazing situation is critical in developing management plans for warm-season tall grasses. A pasture experiment was conducted in 1999, 2000, and 2001 near Mead, NE. The objective of the experiment was to determine the effect of timing and frequency of grazing on big blue- stem (Andropogon gerardii Vitman) etiolated tiller growth and root and rhizome structure. Paddocks were grazed at a stocking rate of 9.9 Animal Unit Month (AUM) ha^-1 in two to four cycles from mid-May to early-September. In April 2002, five 6.6- 132-cm soil cores were …

Portable Probes To Measure Electrical Conductivity And Soil Quality In The Field, S.L. Arnold, John W. Doran, James S. Schepers, Brian J. Wienhold, Daniel Ginting, Brigid Amos, S. Gomes

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Soil electrical conductivity (EC) is a useful indicator in managing agricultural systems, but tools for convenient and inexpensive measurements in the field are generally lacking. Handheld conductivity probes were designed to evaluate in-field naturally occurring and human-induced total soluble electrolyte levels in soil and water. The probes were used to survey and monitor EC in the field and to assess soil and water quality as related to environmental stability and sustainable food production. A pencil-sized 16-cm probe (PP) was connected to a handheld Hanna (DiST WP 4) conductivity meter, resulting in an economical, compact, and easy to use device. The …

K4, K9 And K18 In Human Histone H3 Are Targets For Biotinylation By Biotinidase, Kenya A. Kobza, Gabriela Camporeale, Brian Rueckert, Alice Kueh, Jacob B. Griffin, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Histones are modified post-translationally, e.g. by methylation of lysine and arginine residues, and by phosphorylation of serine residues. These modifications regulate processes such as gene expression, DNA repair, and mitosis and meiosis. Recently, evidence has been provided that histones are also modified by covalent binding of the vitamin biotin. The aims of this study were to identify biotinylation sites in histone H3, and to investigate the crosstalk among histone biotinylation, methylation and phosphorylation. Synthetic peptides based on the sequence of human histone H3 were used as substrates for enzymatic biotinylation by biotinidase; biotin in peptides was probed using streptavidin peroxidase. …

Forage News [2005-08], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• International Grassland Congress
• Grazing School
• Heart of America Grazing Conference
• U.K. All Commodity Field Day
• Alfalfa Hay Acres Up
• Time to Line Up Seed for Fall Planting
• Hay/Barn Fires
• Remember to Stockpile Fescue Starting Now
• USDA-ARS Forage-Animal Production Research Unit
• Field Experience with Strip Grazing Standing Corn as an Economical Alternative for Wintering Beef Cows in the Midwest
• Grazing Corn: Impact of Harvest Date on Yield
• Poultry Litter Rate Study in Tall Fescue
• Upcoming Events

Nebline, August 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

4-H Food Booth at County Fair: Volunteers Have Fun, Rewarding Experience
Support 4-H by Supporting the 4-H Corner Stop
Booth Funds 4-H Council Activities
Household Hints: Energy Saving Ideas
  President’s Notes — Janet’s Jargon
Praise and Encouragement
2005 Scholarship Winner
Pack Your Street Sense When You Pack Your Bags
New Effective Mosquito Repellents Less Greasy, Odor Free
Control Mosquito Populations
Scrap Tire Collection Sept. 24 & 25
Using Natural Enemies to Control Pests
Some Beneficial Predators
Soybean Management Field Day, Aug. 12
Plant Alfalfa in August
St. Johnswort in Lancaster County
Free Soybean Cyst Nematode Sample Kits Available
Ammoniate Wheat …

Molecular Serotyping Of Escherichia Coli O26:H11, Lisa M. Durso, James L. Bono, James E. Keen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Serotyping is the foundation of pathogenic E. coli diagnostics; however, few laboratories have this capacity. We developed a molecular serotyping protocol that targets, genetically, the same somatic and flagellar antigens of E. coli O26:H11 used in traditional serotyping. It correctly serotypes strains untypeable by traditional methods, affording primary laboratories serotyping capabilities.

Sheep Updates 2005 - Part 6, Sarah Pugh, Giles Glasson, Ed Barrett-Lennard, Hayley Norman, Matt Wilmot, Meir Altman, Kelly Pearce, Sally Phelan, David Masters, Nadine Eva, Brad Wooldridge, Stephen Gherardi, Lucy Anderton, Gonzalo Mata, P. Sanford, J Young, Tim Wiley, Richard Quinlan, Chris Oldham, Mike Hyder, Mandy Curnow, Samantha Giles, Andrew Thompson

Sheep Updates

This session covers seven papers from different authors:

PASTURES/GRAZING

1. New annual pastures - quality and quantity for fodder conservation?, Sarah Pugh and Giles Glasson, Department of Agriculture Western Australia

2. Saltland Pastures: Dispelling some Myths, Ed Barrett-Lennard^1,3, Hayley Norman^2,3, Matt Wilmat^2,3, Meir Altman,³, Kelly Pearce^2,3, Sally Phelan⁴, David Masters^2,3, ^1. Department of Agriculture, Western Australia, ² CSIRO Livestock Industries, Floreat, WA, ³. CRC for Plant-based Management of Dryland Salinity ⁴. Saltland Pastures Association

3. Pastures: Putting profit back into sandplain, Nadine Eva, Department …

Sheep Updates 2005 - Part 3, Rob Davidson, David Pethick, C. F. Engelke, B. D. Siebert, K. Gregg, A-D G. Wright, P. E. Vercoe, Robin Jacob, Russell Barnett, Joanne Sneddon, Sandra Brown, G. Rose, C. Kabore, J Dart, Stuart Adams

Sheep Updates

This session covers seven papers from different authors: CUSTOMER 1. Benefits VIAscanR to producers and WAMMCO, Rob Davidson, Supply Development Manager, David Pethick, School of Veterinary and Biomedical Studies, Murdock University. 2. Healthy fats in lamb: how WA lambs compare with others, C. F. Engelke Animal Biology, University of Western Australia, bCSIRO Livestock Industries, Western Australia B.D. Siebert, Department of Animal Science, University of Adelaide, South Australia, K. Gregg, Centre for High-Throughput Agricultural Genetic Analysis, Murdoch University, Western Australia. A-D.G. Wright CSIRO Livestock Industries, Western Australia, P.E Vercoe Animal Biology, University of Western Australia 3. Shelf life of fresh lamb …

Evaluating Modeling Techniques For Cattle Heat Stress Prediction, T. M. Brown-Brandl, David D. Jones, Wayne Woldt

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Researchers have traditionally predicted animal responses by means of statistical models. This study was conducted to evaluate modeling techniques. One hundred and twenty-eight feedlot heifers were observed during a 2-month period during the summer of 2002. Respiration rate and surface temperature were taken on a random sample of 40 animals twice a day. Five different models (two statistical models, two fuzzy inference systems, and one neural network) were developed using 70% of this data, and then tested using the remaining 30%. Results showed that the neural network described the most variation in test data (68%), followed by the data-dependent fuzzy …

Center For Grassland Studies Newsletter, Summer 2005, Volume 11, No. 3

Center for Grassland Studies: Newsletters

Why Do We All Work So Hard on Our Lawns? Blame Habit, Snobbery by Cynthia Crosses

Intensify Extensive Beef Systems? by Terry Klopfenstein, Jeff Folmer, and Galen Erickson

CGS Citizens Advisory Council and Associates Visit Southwestern Nebraska

Audubon’s First Important Bird Areas in Nebraska Announced

XX International Grassland Congress by Walter Schacht, Department of Agronomy, UNL

Visit the Ecological Site Information System by George Peacock, Natural Resources Conservation Service, Central National Technology Support Center, Fort Worth, Texas

Opportunity for Holistic Management Training

Nebline, July 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Avoid Portion Distortion With MyPyramid’s Specific Guidelines
Mandevilla — Tropical Vine with Showy Flowers
Fragrance in the Garden
Garden Guide: Things to do this month
Most Leaf Galls Don’t Harm Trees
Traveling This Summer? Find Bedbugs in Hotel Rooms Before They Find You!
Household Hazardous Waste Collections, June 24 & 25
Some Insects Thrive on Rat Poison
P3 Intern Working at Extension
Pine Wilt Disease
How to Sample for Pinewood Nematode
Pine Species to Avoid Planting
Presentation on Sustainable Agriculture, July 8
Predicting the Last Irrigation for Corn, Milo and Soybeans
Irrigating Summer Turf
In Hot Weather, Raise Mower Height …

Fertilizer, Tillage, And Dairy Manure Contributions To Nitrate And Herbicide Leaching, C. S. Stoddard, John H. Grove, Mark S. Coyne, William O. Thom

Plant and Soil Sciences Faculty Publications

Few studies have examined the water quality impact of manure use in no-tillage systems. A lysimeter study in continuous corn (Zea mays L.) was performed on Maury silt loam (fine, mixed, semiactive, mesic Typic Paleudalf) to evaluate the effect(s) of tillage (no-till [NT] and chisel-disk [CD]), nitrogen fertilizer rate (0 and 168 kg N ha⁻¹), and dairy manure application timing (none, spring, fall, or fall plus spring) on NO₃–N, atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine), and alachlor [2-chloro-2′-6′-diethyl-N-(methoxymethyl)acetanilide] concentrations in leachate collected at a 90-cm depth. Herbicides were highest immediately after application, declining to less …

Plant Virus Hc-Pro Is A Determinant Of Eriophyid Mite Transmission, Drake C. Stenger, Gary L. Hein, Frederick E. Gildow, Kempton M. Horken, Roy C. French

Department of Plant Pathology: Faculty Publications

The eriophyid mite transmitted Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) shares a common genome organization with aphid transmitted species of the genus Potyvirus. Although both tritimoviruses and potyviruses encode helper component-proteinase (HC-Pro) homologues (required for nonpersistent aphid transmission of potyviruses), sequence conservation is low (amino acid identity, ~16%), and a role for HC-Pro in semipersistent transmission of WSMV by the wheat curl mite (Aceria tosichella [Keifer]) has not been investigated. Wheat curl mite transmissibility was abolished by replacement of WSMV HC-Pro with homologues of an aphid transmitted potyvirus (Turnip mosaic virus), …

Pineywoods Native Plant Center, July 2005, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Newsletters

No abstract provided.

Loss Of Phosphorus By Runoff For Agricultural Watersheds, M. A. Elrashidi, M. D. Mays, J. Harder, D. Schroeder, P. Brakhage, S. Peaslee, C. Seybold, C. Schaecher

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The loss of nutrients in runoff from agricultural land is a major cause of poor surface water quality in the United State. Scientists (NRCS) developed a technique to estimate the impact of agricultural watersheds on natural water resources. The objectives of this study were to apply this technique on the Wagon Train (WT),watershed to predict (1) loss of water by surface runoff, (2) loss of phosphorus (P) from soils by runoff and P loading for WT reservoir. The annual loss of water by runoff was estimated at 4.32 million m³ . The USGS data for a 50-year period (1951 …

Planning Your Landscape Microclimates, Seasons, Sites And Soils, Larry A. Sagers

All Archived Publications

No abstract provided.

Advanced Master Gardener Landscape Gardening For Gardeners, Larry A. Sagers

All Archived Publications

No abstract provided.

Abiotic Flower Diseases, Larry A. Sagers

All Archived Publications

No abstract provided.

Introduction To Taxonomy, Larry A. Sagers, Gretchen Campbell

All Archived Publications

No abstract provided.

High-Throughput Immunoblotting Identifies Biotin-Dependent Signaling Proteins In Hepg2 Hepatocarcinoma Cells1, Rocio Rodriguez-Melendez, Jacob B. Griffin, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Biotin affects the abundance of mRNA coding for ~10% of genes expressed in human-derived hepatocarcinoma (HepG2) cells. Here, we determined whether effects of biotin on gene expression are associated with changes in the abundance of distinct proteins in cell signaling and structure. HepG2 cells were cultured in media containing the following concentrations of biotin: 0.025 nmol/L (denoted “deficient”), 0.25 nmol/L (“physiological” = control), and 10 nmol/L (“pharmacological”) for 10 d before harvesting. The abundance of 1009 proteins from whole-cell extracts was quantified by using high-throughput immunoblots. The abundance of 44 proteins changed by at least 25% in biotin-deficient and biotin-supplemented …

Pseudomonas Syringae Type Iii Chaperones Shco1, Shcs1, And Shcs2 Facilitate Translocation Of Their Cognate Effectors And Can Substitute For Each Other In The Secretion Of Hopo1-1, Ming Guo, Scott T. Chancey, Fang Tian, Zhengxiang Ge, Yashitola Jamir, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The Pseudomonas syringae type III secretion system (TTSS) translocates effector proteins into plant cells. Several P. syringae effectors require accessory proteins called type III chaperones (TTCs) to be secreted via the TTSS. We characterized the hopO1-1, hopS1, and hopS2 operons in P. syringae pv. tomato DC3000; these operons encode three homologous TTCs, ShcO1, ShcS1, and ShcS2. ShcO1, ShcS1, and ShcS2 facilitated the type III secretion and/or translocation of their cognate effectors HopO1-1, HopS1, and HopS2, respectively. ShcO1 and HopO1-1 interacted with each other in yeast two-hybrid and coimmunoprecipitation assays. Interestingly, ShcS1 and ShcS2 were capable of substituting for …

Forage News [2005-06], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• UK College of Agriculture Research Farm Celebrates 50 Years
• Maintaining Healthy Horse Pastures (New Poster)
• Barren County Continued as Cattle Capital of Kentucky
• Toward Higher Quality Hay
• Harvest and Storage Method Affects Ergot Alkaloid Concentration in Tall Fescue
• Intake, Digestion, and N Metabolism in Steers Fed Endophyte-Free, Ergot Alkaloid-Producing Endophyte-Infected, or Nonergot-Alkaloid Producing Endophyte-Infected Fescue Hay
• Upcoming Events

Grazing Intensity Effects On Northern Plains Mixed-Grass Prairie, Wendi M. Rogers, Donald R. Kirby, Paul E. Nyren, Bob D. Patton, Edward S. Dekeyser

The Prairie Naturalist

We evaluated the effects of long-term (1988 to 2000) grazing on northern mixed-grass prairie at tI.e Central Grasslands Research Extension Center in south-central North Dakota. We did not detect a difference in herbaceous basal cover between grazing intensities following 12 consecutive years of season-long moderate (50% removal of annual above-ground standing crop) and heavy (80% removal of annual above-ground standing crop) grazing. However, both moderate and heavy grazing intensities reduced above-ground herbaceous standing crop, total root biomass, and soil organic carbon. Moderate grazing intensity maintained a greater amount of deep (10 to 20 cm) and total root biomass relative to …