Digital Commons Network^™

Fish And Wildlife Benefits Associated With Wetland Establishment Practices, Charles A. Rewa

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Efforts to establish wetlands through restoration and creation actions have increased in recent decades in response to regulatory and voluntary incentive programs. This paper summarizes the findings of studies conducted to document fish and wildlife response to these practices. The majority of published studies describe bird response to wetland restoration, with most reporting bird communities in restored wetlands to be similar to those of natural reference wetlands. Studies also indicate that invertebrates and amphibians generally respond quickly to and colonize newly established wetland habitats. Key factors reported as correlated with wildlife species richness include wetland size, availability of nearby wetlands …

Feed Grains Backgrounder, Linwood Hoffman, Allen Baker, Linda Foreman, C. Edwin Young

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The U.S. feed grain sector, largest of the major U.S. field crops, faces unprecedented demand conditions. The size and speed of the expanding use of corn by the ethanol industry is raising widespread issues throughout the U.S. agricultural sector. Debate is ongoing over the use of grain for fuel instead of for food or feed and the adequacy of future grain supplies. Increased productivity (yield) and additional area from land planted to competing crops, land enrolled in conservation programs, or idled land is expected to provide an increased supply of feed grains. In 2003, U.S. feed grain farms had an …

Effect Of Drying On Phosphorus Distribution In Poultry Manure, Heidi W. Dail, Zhongqi He, M. Susan Erich, C. Wayne Honeycutt

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Laboratory drying may alter manure phosphorus (P) distribution. The effects of freeze, air (22 °C), and oven (65 °C) drying on sequentially fractioned poultry manure P were examined. Higher drying temperatures resulted in lower percentage of dry matter. Increased H₂O- and decreased sodium bicarbonate (NaHCO₃)-extractable P with drying provided evidence that drying increases poultry manure P solubility. Labile fractions were predominantly inorganic P (P_i), whereas sodium hydroxide (NaOH) and hydrochloric acid (HCl) fractions had significant amounts of organic P (P_o). Drying altered H₂O- and NaHCO₃-extractable P_i but …

Efficiency Of Gene Silencing In Arabidopsis : Direct Inverted Repeats Vs. Transitive Rnai Vectors, Sergei A. Filichkin, Stephen P. Difazio, Amy M. Brunner, John M. Davis, Zamin K. Yang, Udaya C. Kalluri, R. S. Arias, Elizabeth Etherington, Gerald A. Tuskan, Steven H. Strauss

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We investigated the efficiency of RNA interference (RNAi) in Arabidopsis using transitive and homologous inverted repeat (hIR) vectors. hIR constructs carry self-complementary intron-spliced fragments of the target gene whereas transitive vectors have the target sequence fragment adjacent to an intron-spliced, inverted repeat of heterologous origin. Both transitive and hIR constructs facilitated specific and heritable silencing in the three genes studied (AP1 , ETTIN and TTG1). Both types of vectors produced a phenotypic series that phenocopied reduction of function mutants for the respective target gene. The hIR yielded up to fourfold higher proportions of events with strongly manifested reduction …

Classification Of Dry-Milled Maize Grit Yield Groups Using Quadratic Discriminant Analysis And Decision Tree Algorithm, Kyung-Min Lee, Timothy J. Herrman, Scott R. Bean, David S. Jackson, Jane Lingenfelser

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A genetically and environmentally diverse collection of maize (Zea maize L.) samples was evaluated for physical properties and grit yield to help develop a standard set of criteria to identify grain best suited for dry milling. Application of principal component analysis (PCA) reduced a set of approximately 500 samples collected from six states to 154 maize hybrids. Selected maize hybrids were placed into seven groups according to their dry-milled grit yields. Regression analysis explained only 50% of the variability in dry-milling grit yield. Patterns of differences in the physical properties for the seven grit yield groups implied that the …

Growth And Pubertal Development Of F1 Bulls From Hereford, Angus, Norwegian Red, Swedish Red And White, Friesian, And Wagyu Sires, E. Casas, D. Lunstra, Larry V. Cundiff, Johny Joe Ford

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The objective of the study was to characterize body growth, testicular development, and puberty from 8 to 14 mo of age in bulls (n = 120) produced by mating sires from Hereford, Angus, Norwegian Red, Swedish Red and White, Friesian, and Wagyu breeds to MARC III (¹⁄₄ Hereford, ¹⁄₄ Angus, ¹⁄₄ Red Poll, and ¹⁄₄ Pinzgauer) cows. Traits evaluated were birth weight, weaning weight (at 215 d), yearling weight, ADG from 8 to 14 mo of age, paired testicular volume growth from 8 to 14 mo of age, age at puberty (determined by production of 50 × 10⁶ sperm …

The Original Usda-Ars Experimental Watersheds In Texas And Ohio: Contributions From The Past And Visions For The Future, R. D. Harmel, J. V. Bonta, C. W. Richardson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The USDA Soil Conservation Service (USDA‐SCS) realized the importance of understanding hydrologic processes on agricultural fields and watersheds in the mid‐1930s. Based on this realization, the research program of the Hydrologic Division of SCS established three experimental watersheds across the U.S. to analyze the impact of landuse practices on soil erosion, flood events, water resources, and the agricultural economy. Two of the original watersheds remain in operation today within the USDA Agricultural Research Service (USDA‐ARS): the Blacklands Experimental Watershed (now the Grassland, Soil and Water Research Laboratory) near Riesel, Texas, and the North Appalachian Experimental Watershed near Coshocton, Ohio. These …

Design And Testing Of A Closed, Stirring Respirometer For Measuring Oxygen Consumption Of Channel Catfish Eggs, Eugene L. Torrans

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A closed, stirring respirometer was designed to agitate small egg mass samples of channel catfish Ictalurus punctatus while measuring oxygen consumption by the eggs. Egg mass samples ranging in size from 15 to 46 g were placed on a screen platform above a magnetic stir bar in the respirometer; the degree of agitation was controlled with a magnetic stir plate. Dissolved oxygen (DO) concentration and temperature were measured with a luminescent DO sensor and meter. The respirometer was used to determine routine metabolic rate and limiting oxygen concentration (the presumed hypometabolic transition zone) on egg mass samples from 10 different …

Techniques For Reclaiming Mined Lands With Native Plants, Maria Elena Melnechuk

Theses and Dissertations

In this experiment I examined the role of mycorrhizae, seeding rate, and fertilizer in promoting growth of native plants at a reclaimed mine site in central Arkansas. Permanent field plots were established to examine effects of treatments on native plant cover, nonnative plant cover, and bare ground. By the final year of data collection, 18 native species had been newly established at the experimental site. Compared to plots without mycorrhizae, plots with mycorrhizae had significantly higher native plant cover in the first sampling year only. The addition of native seed was important—plots without added seed had significantly lower native plant …

Nebline, January 2007

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

UNL College of Ag and Natural Resources Turns Talents into Rewarding Careers
Crop Protection Clinic, Jan. 22
Glyphosate-Resistant Marestail Confirmed in Nebraska
Upcoming Pesticide Applicator Trainings
Rethinking Shelterbelts
Salt Damage to Plants Determining Houseplant Problems
Girdling Roots on Trees
Winter Strategies for Wildlife
Where Do Insects Go in the Winter?
Lifelong Learning in Nutrition
Make-Ahead Frozen Beef “Crumbles” Recipe
Peanut Butter Banana Shake Recipe
Are You a “Lark” or an “Owl”? Cook Ahead Accordingly!
Play “FOOD” Ball Online!
President’s Notes — Alice’s Analysis
Household Hints
FCE News & Events
Teach Your Children Well
10 Ways to Save with Lighting
Strengthening …

2007 Spring Seed Book, Nebraska Crop Improvement Association

Nebraska Crop Improvement Association Seed Guides

FOUNDATION SEED

PLANT VARIETY PROTECTION ACT and HOW IT BENEFITS YOU!

PERENNIAL FORAGE GRASSES

TURFGRASSES

TURFGRASS SOD

SOYBEANS

SOYBEAN VARIETY CHARACTERISTICS - 2007

OAT VARIETY CHARACTERISTICS - 2007

OATS

SPRING BARLEY

FIELD PEAS

MILLET

MILLET VARIETY CHARACTERISTICS 2007

HYBRID SEED CORN

NEBRASKA SEED QUALITY ASSURANCE® PROGRAM

SOYBEANS BUYERS’ NOTICE

APPROVED SEED CONDITIONERS

CUSTOM CERTIFIED CONDITIONERS

2006 NCIA MEMBERS

A Novel Endonuclease Activity Associated With The Arabidopsis Ortholog Of The 30-Kda Subunit Of Cleavage And Polyadenylation Specificity Factor, Balasubrahmanyam Addepalli, Arthur G. Hunt

Plant and Soil Sciences Faculty Publications

The polyadenylation of messenger RNAs is mediated by a multi-subunit complex that is conserved in eukaryotes. Among the most interesting of these proteins is the 30-kDa-subunit of the Cleavage and Polyadenylation Specificity Factor, or CPSF30. In this study, the Arabidopsis CPSF30 ortholog, AtCPSF30, is characterized. This protein possesses an unexpected endonucleolytic activity that is apparent as an ability to nick and degrade linear as well as circular single-stranded RNA. Endonucleolytic action by AtCPSF30 leaves RNA 3′ ends with hydroxyl groups, as they can be labeled by RNA ligase with [³²P]-cytidine-3′,5′-bisphosphate. Mutations in the first of the three CCCH …

Letter From The Dean, Gregory J. Weidemann

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 8 2007, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Multifunctional Rural Landscapes: Economic, Environmental, Policy, And Social Impacts Of Land Use Changes In Nebraska, Twyla M. Hansen, Charles A. Francis, J. Dixon Esseks, J. Allen Williams Jr.

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The conversion of farmland near cities to other human uses is a global trend that challenges our long-term capacity to provide food, fiber, and ecosystem services to a growing world population. If current trends continue in the United States, the population will reach 450 million by the year 2050. At the same time, an accelerating change in land use will reduce today’s two acres per person of farmland to less than one acre per person. This is scarcely enough to produce food for our domestic population, without any food available for export – even assuming advances in technology. We need …

Tungoil Tree (Aleurites Fordii Hemsl.) (Euphorbiaceae): New To The Arkansas Flora, Brett Serviss, Nicole Freeman, Joslyn Hernandez, Allen Leible, Chris Talley

Journal of the Arkansas Academy of Science

No abstract provided.

Antibacterial Activity Of Apis Mellifera L. Propolis Collected In Three Regions Of Kenya, Elliud M. Muli, J. M. Maingi

All Peer-Reviewed Publications

The present study aimed at investigating the susceptibility of the microorganisms Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis to ethanolic extracts of propolis (EEP) from three regions of Kenya (Taita, Tana and Samburu). Propolis was extracted using four different concentrations of ethanol: pure, 70%, 50%, and 30%. Ethanol (70%) and Streptomycin were used as controls. The agar diffusion method using filter paper disks was employed. Antibacterial activity was determined as an equivalent of the inhibition zones diameters (in millimeters) after incubation at 37°C for 24h. Significant differences in the antibacterial activities of propolis were observed among …

Comparison Of Instrumental Methods For Measuring Seed Hardness Of Food-Grade Soybean, Mioko Tamura, Bo Zhang, Joyce Berger-Doyle, Pengyin Chen

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Seed hardness is an important factor in determining soybean suitability for natto production. There is no established methodology for testing seed texture of soybeans. The objective of this study was to develop an efficient method by examining different instruments and seed parameters that could be potentially used for testing soybean seed hardness. Five food-grade soybean genotypes with different seed sizes were used to determine seed hardness and water-absorption capacity. Water absorption capacity was expressed by swell ratios for seed weight, seed dimension, and volume of water changes before and after soaking. Seed hardness test was conducted by a one-bite method …

Landholder Information For The Safe Use And Management Of Strychnine For Emu Control, Renee Donovan, David Goodall

Biosecurity published reports

Farmnote 34/2003 - Guide to safe use of strychnine poison for emu control included.

Strychnine is a highly poisonous substance that is used in Western Australia for the control of Wild Dogs and Emus. Its use is restricted by law.

This manual outlines your roles and responsibilities in regard to Strychnine use for emu control. In particular this manual outlines the essential information you need to know in regard to:

• The legislative requirements for Strychnine control in Western Australia
• The properties and mode of action of Strychnine
• How to apply to have a strychnine emu baiting program carried out on …

Landholder Information For The Safe Use And Management Of 1080, Renee Donovan, David Goodall

Biosecurity published reports

This manual outlines your roles and responsibilities for using and managing 1080. In particular this manual outlines the essential information you need to know in regard to:

• The legislative requirements for 1080 control in Western Australia
• The properties and mode of action of 1080
• How to lodge an application to lay 1080 baits How to safely store and transport 1080
• The procedures and notification required to carry out a baiting proposal
• Personal safety, identifying signs of poisoning & effecting appropriate first aid treatment
• Your responsibilities for notifying DAFWA and the Police of any accidents or incidents
• Methods for evaluating the …

A Soybean Transcript Map: Gene Distribution, Haplotype And Single-Nucleotide Polymorphism Analysis, Ik-Young Choi, D. L. Hyten, Lakshmi K. Matukumalli, Qijian Song, Julian M. Chaky, Charles V. Quigley, Kevin Chase, K. Gordon Lark, Robert S. Reiter, Mun-Sup Yoon, Eun-Young Hwang, Seung-In Yi, Nevin D. Young, Randy C. Shoemaker, Curtis P. Van Tassell, James E. Specht, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

The first genetic transcript map of the soybean genome was created by mapping one SNP in each of 1141 genes in one or more of three recombinant inbred line mapping populations, thus providing a picture of the distribution of genic sequences across the mapped portion of the genome. Singlenucleotide polymorphisms (SNPs) were discovered via the resequencing of sequence-tagged sites (STSs) developed from expressed sequence tag (EST) sequence. From an initial set of 9459 polymerase chain reaction primer sets designed to a diverse set of genes, 4240 STSs were amplified and sequenced in each of six diverse soybean genotypes. In the …

Adaptations Of Five Pale-Type Grain Amaranth Accessions (Amaranthus Caudatus) And Five Ataco Or Sangorache Accessions (Amaranthus Hybridus) In The Cities Of Otavalo And Antonio Ante, Sixto Stalin Tuston Torres

Theses and Dissertations

This study was conducted in two locations: Pucará (2,413m above sea level) and Mojandita (2,922m above sea level), both of which are found in the province of Imbabura, Ecuador. The objectives of this study were to: characterize the morphological and agronomic traits as well as the overall quality of the ten amaranth accessions in this study; identify the accessions with the highest yield and biomass; determine the nutritional characteristics of the experimental accessions; and determine the cost of production of amaranth per hectare. The following null hypothesis was assumed: the agronomic and nutritional characteristics of the ten accessions in this …

Mycotoxin Occurrence And Aspergillus Flavus Soil Propagules In A Corn And Cotton Glyphosate-Resistant Cropping Systems, K. N. Reddy, H. K. Abbas, R. M. Zablotowicz, C. A. Abel, C. H. Koger

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The effects of cotton–corn rotation and glyphosate use on levels of soil-borne Aspergillus flavus, aflatoxin and fumonisin contamination in corn and cotton seed were determined during 2002–2005 in Stoneville, Mississippi (USA). There were four rotation systems (continuous cotton, continuous corn, cotton–corn and corn–cotton) for both glyphosate-resistant (GR) and non-GR cultivars–herbicide system arranged in a randomized complete block design with four replications. Aspergillus flavus populations in surface (5-cm depth) soil, sampled before planting (March/April), mid-season (June) and after harvest (September), ranged from 1.47 to 2.99 log (10) cfu g^-1 soil in the four rotation systems. Propagules of A. flavus …

First Report Of Yersinia Ruckeri Biotype 2 In The Usa, Cova R. Arias, Oscar Olivares-Fuster, Karl Hayden, Craig A. Shoemaker, John M. Grizzle, Phillip H. Klesius

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A polyphasic characterization of atypical isolates of Yersinia ruckeri (causative agent of enteric redmouth disease in trout) obtained from hatchery-reared brown trout Salmo trutta in South Carolina was performed. The Y. ruckeri isolates were biochemically and genetically distinct from reference cultures, including the type strain, but were unequivocally ascribed to the species Y. ruckeri, based on API 20E, VITEK, fatty acid methyl ester profiles, and 16S rRNA gene sequencing analysis. These isolates were nonmotile and unable to hydrolyze Tween 20/80 and were therefore classified as Y. ruckeri biotype 2. Genetic fingerprint typing of the isolates via enterobacterial repetitive intergenic …

Mineralization Of Nitrogen In Soils Amended With Dairy Manure As Affected By Wetting/Drying Cycles, D. B. Watts, H. A. Torbert, S. A. Prior

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Interest in manure management and its effects on nitrogen (N) mineralization has increased in recent years. The focus of this research was to investigate the N-mineralization rates of different soil types in Coastal Plain soils and compare them to a soil from Illinois. Soils with and without dairy composted manure addition were subjected to different wetting/drying cycles [constant moisture at 60% waterfilled pore space (WFPS) and cycling moisture from 60 to 30% WFPS] under laboratory conditions at three different temperatures (11 °C, 18 °C, and 25 °C). Samples were collected from three different soil types: Catlin (Mollisols), Bama (Ultisols), and …

Non-Composted Municipal Solid Waste Processing Byproduct Effect On Soil Reclamation, H. Allen Torbert, Dick L. Gebhart, Ryan R. Busby, Kenneth N. Potter, Deborah R. Curtain

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A new garbage processing technology has been developed that sterilizes and separates inorganic and organic components of municipal solid waste. The non-composted byproduct of this process, Fluff ®, has the potential to be utilized as a soil amendment to improve soil conditions in highly degraded soils. A study was initiated to evaluate Fluff as a soil amendment for establishing native grasses on disturbed US Army training lands. The Fluff was incorporated into a sandy loam soil at Fort Benning Military Reservation, GA on two sites: a moderately degraded and a highly degraded soil. The Fluff was incorporated at rates of …

Alkaline Phosphatase Reactivity In The Vagina And Uterovaginal Junctionsperm-Storage Tubules Of Turkeys In Egg Production: Implications Forsperm Storage, M. R. Bakst, V. Akuffo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

1. Currently there remains contradictory intortliation on the localisation and possible roleof alkaline phosphatase (AP) in the chicken and Japanese quail oviducts.

2.Using turkeys with a hard-shelled egg in their uteri, vaginal and uterovaginal junction mucosae werestretched and fixed as whole mounts prior to the Imistochemical localisation of All activity.

3.Scattered All reactive cells were observed in the vaginal and uteiovaginal junction surface epitheliaand intense All reactivity of the sperm-storage tubule (SST) epithelium, localised to its apical border.

4.We suggest that such All in hens in egg production may reflect cell dif.ftreiitiation andproliferation in the vagina and SST and possibly …

Adrenomedullin (Am) And Adrenomedullin Binding Protein (Am-Bp) In The Bovine Mammary Gland And Milk: Effects Of Stage Of Lactation And Experimental Intramammary E. Coli Infection, Ted H. Elsasser, Anthony V. Capuco, Thomas J. Caperna, Alfredo Martínez, Frank Cuttitta, Stanislaw Kahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Adrenomedullin (AM) has been characterized as an endogenous tissue survival factor and modulator of many inflammatory processes. Because of the increased susceptibility of the mammary gland to infection during the time surrounding parturition in the cow, we investigated how milk and tissue content of AM and its binding protein (AM-BP) might be affected by the stage of lactation and the udder health status. Milk and mammary biopsy samples were obtained from Holstein cows 21 days prior to and at various times after calving to represent the dry period and early and midstages of lactation. Additional cows received an intramammary challenge …

Identification And Characterization Of Aquaporin-9 (Aqp9) In Porcine Hepatic Tissue And Hepatocytes In Monolayer Culture, Thomas J. Caperna, Amy E. Shannon, Mark P. Richards, Wesley M. Garrett, Neil C. Talbot

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aquaporins (AQPs) are members of a large family of integral membrane proteins involved in the rapid movement of water and neutral solutes across cell membranes. In this study, we have prepared an affinity-purified porcine-specific polyclonal antiserum to AQP9 and have investigated the distribution and expression of AQP9 in pig liver tissue and in hepatocytes in primary culture. Immunocytochemical analysis showed that AQP9 was primarily localized in the membrane structures of hepatocytes and was not associated with intrahepatic bile ducts or blood vessels. Western blot analysis indicated that AQP9 ranged in apparent molecular mass between 27 and 38 kD in whole …

Isolation And Characterization Of Porcine Visceral Endoderm Cell Lines Derived From In Vivo 11-Day Blastocysts, Neil C. Talbot, Le Ann Blomberg, Ayesha Mahmood, Thomas J. Caperna, Wesley M. Garrett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two porcine cell lines of yolk-sac visceral endoderm, designated as PE-1 and PE-2, were derived from in vivo 11-d porcine blastocysts that were either ovoid (PE-1) or at the early tubular stage of elongation (PE-2). Primary and secondary culture of the cell lines was done on STO feeder cells. The PE-1 and PE-2 cells morphologically resembled visceral endoderm previously cultured from in vivo-derived ovine and equine blastocysts and from in vitro-derived bovine blastocysts. Analysis of the PE-1- and PE-2-conditioned medium by 2D-gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flightmass spectrometry demonstrated that they produced serum proteins. Reverse transcriptase polymerase chain …