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Bcl-2 Family Members Inhibit Oxidative Stress-Induced Programmed Cell Death In Saccharomyces Cerevisiae, Shao-Rong Chen, David Dunigan, Martin Dickman

Department of Plant Pathology: Faculty Publications

Selected antiapoptotic genes were expressed in baker’s yeast (Saccharomyces cerevisiae) to evaluate cytoprotective effects during oxidative stress. When exposed to treatments resulting in the generation of reactive oxygen species (ROS), including H₂O₂, menadione, or heat shock, wild-type yeast died and exhibited apoptotic-like characteristics, consistent with previous studies. Yeast strains were generated expressing nematode ced-9, human bcl-2, or chicken bcl-xl genes. These transformants tolerated a range of oxidative stresses, did not display features associated with apoptosis, and remained viable under conditions that were lethal to wildtype yeast. Yeast strains expressing a mutant antiapoptotic gene (bcl-2 …

Induced Resistance As A Mechanism Of Biological Control By Lysobacter Enzymogenes Strain C3, Ozlem Kilic-Ekici, Gary Y. Yuen

Department of Plant Pathology: Faculty Publications

Induced resistance was found to be a mechanism for biological control of leaf spot, caused by Bipolaris sorokiniana, in tall fescue (Festuca arundinacea) using the bacterium Lysobacter enzymogenes strain C3. Resistance elicited by C3 suppressed germination of B. sorokiniana conidia on the phylloplane in addition to reducing the severity of leaf spot. The pathogen-inhibitory effect could be separated from antibiosis by using heat-inactivated cells of C3 that retained no antifungal activity. Application of live or heat-killed cells to tall fescue leaves resulted only in localized resistance confined to the treated leaf, whereas treatment of roots resulted in …

Evolution Of Wheat Streak Mosaic Virus: Dynamics Of Population Growth Within Plants May Explain Limited Variation, Roy C. French, Drake C. Stenger

Department of Plant Pathology: Faculty Publications

Like many other plant RNA viruses, Wheat streak mosaic virus (WSMV) sequence diversity within and among infected plants is low given the large number of virions produced. This may be explained by considering aspects of plant virus life history. Intracellular replication of RNA viruses is predominately linear, not exponential, which means that the rate at which mutations accumulate also is linear. Bottlenecks during systemic movement further limit diversity. Analysis of mixed infections with two WSMV isolates suggests that about four viral genomes participate in systemic invasion of each tiller. Low effective population size increases the role of stochastic processes on …

Review Of Molecular Biology Of Fungal Development, Mycology Series, Volume 15, Marty Dickman

Department of Plant Pathology: Faculty Publications

Numerous publications, books, and textbooks are available that treat the subject of developmental biology. Far less is available detailing the fungi, an important group of organisms that have implications in a broad variety of contexts from medical to agricultural to food, and of course as model systems for “higher” eukaryotes. This nicely edited book by Osiewacz is an attempt to fill that gap. He has compiled an impressive list of authors, who represent leaders in their respective fields. This gives readers a detailed and current view of a broad array of fungal processes and lifestyles, ranging from saprophytes to pathogens …

Crop Updates - 2003 Pulses, M. Harries, R. French, I. Pritchard, R. Beermier, N. Poulish, S. White, M. Seymour, P. White, T. Pope, M. Blyth, L. Young, N. Abbas, J. Plummer, K. Siddique, D. Harris, T. Khan, K. Regan, G. P. Riethmuller, B. Macleod, J. Berger, N. Turner, F. Shan, H. Clarke, P. Smith, M. Baker, H. Dhammu, T. Piper, D. Nicholson, M. Materne, J. Howieson, R. Yates, S. Loss, Z. Rengel, B. Bowden, M. Bolland, J. Croser, A. Harrod, J. Galloway, C. Beeck, J. Wroth, W. Cowling, M. Salam, A. Diggle, R. Jones, B. Coutts, L. Smith, O. Byrne, D. Hardie, J. Ridsill-Smith, H. Sharma, K. Mann, S. Kelly, S. Sharma, H. Hunter, V. Vanstone

Crop Updates

This session covers fifty one papers from different authors

2002 PULSE INDUSTRY HIGHLIGHTS

CONTRIBUTORS

BACKGROUND

2002 REGIONAL ROUNDUP

1.Northern Agricultural Region, M. Harries, Department of Agriculture

2.Central agricultural Region, R. French and I. Pritchard, Department of Agriculture

3.Great Southern and Lakes, R. Beermier, N. Poulish and S. White, Department of Agriculture

4.Esperance Mallee, M. Seymour, Department of Agriculture

PULSE PRODUCTION ECONOMY AND GENETIC IMPROVEMENT

5.Faba Bean, P. White, Department of Agriculture

6.Germplasm evaluation, P. White, T. Pope, M. Harries and M. Seymour, Department of Agriculture

7.Row spacing and sowing rate, M. Seymour, Department …

Crop Updates - 2003 Oilseeds, Peter Nelson, Graham Walton, Hasan Zaheer, Paul Carmody, J. A. Fortescue, D. W. Turner, Janet Wroth, Wallace Cowling, Anh-Van Pham, R. F. Brennan, Glen Riethmuller, Greg Hamilton, Jo Hawksley, C. M. Zaicou-Kunesch, D. Eksteen, Françoise A. Berlandier, Christiaan Valentine, Owain Edwards, Roger Jones, Brenda Coutts, Lisa Smith, Jenny Hawkes, Ravjit Khangura, Moin Salam, Art J. Diggle, Martin J. Barbetti, Kevin Walden

Crop Updates

This session covers fifteen papers from different authors

ACKNOWLEDGMENTS

VARIETIES

1. Large scale canola varietal evaluation in WA, Peter Nelson, Oilseeds WA

2. Performance of IT and TT canola varieties in the medium and high rainfall agzones of WA 2001-02, Graham Walton, Hasan Zaheer and Paul Carmody, Department of Agriculture

QUALITY

1. Reproductive biology, cotyledon development and oil accumulation in canola, J.A. Fortescue and D.W. Turner, School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia

2. Plant and environmental factors affecting oil concentration in canola – a mini-review, D.W. Turner, School of Plant Biology, …

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Crop Updates 2003 - Cereals, Graham Crosbie, Robert Loughman, Collin Wellings, Greg Shea, Simon Mckirdy, Neil C. Turner, Brenda Shackley, Wal Anderson, Darshan Sharma, Mohammad Amjad, Steve Penny Jr, Melanie Kupsch, Anne Smith, Veronika Reck, Pam Burgess, Glenda Smith, Elizabeth Tierney, Peter Burges, Moin U. Salam, Megan Collins, Art Diggle, Blakely Paynter, Roslyn J. Jettner, Kevin Young, Jocelyn Ball, Natasha Littlewood, Lucy Anderton, Irene Waters, Tim Setter, Jeff Russell, Reg Lance, Chengdao Li, Sue Broughton, Michael Jones, Grace Zawko, Keith Gregg, Stephen Loss, Frank Ripper, Ryan Guthrie, Daniel Bell, Patrick Gethin, Narelle Hill, Laurence Caeslake, Vivien Vanstone, Sean Kelly, Helen Hunter, Christopher R. Newman

Crop Updates

This session covers twenty one papers from different authors:

PLENARY

1. Recognising and responding to new market opportunities in the grains industry, Graham Crosbie, Manager, Grain Products Research, Crop Breeding, Plant Industries, Department of Agriculture

2. Stripe rust – where to now for the WA wheat industry? Robert Loughman¹, Colin Wellings² and Greg Shea¹¹Department of Agriculture, ²University of Sydney Plant Breeding Institute, Cobbitty (on secondment from NSW Agriculture)

3. Benefits of a Grains Biosecurity Plan, Dr Simon McKirdy, Plant Health Australia, Mr Greg Shea, Department of Agriculture

4. Can we improve …

Unusual Life Style Of Giant Chlorella Viruses, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Paramecium bursaria chlorella virus (PBCV-1) is the prototype of a family of large, icosahedral, plaque-forming, dsDNA viruses that replicate in certain unicellular, eukaryotic chlorella-like green algae. Its 330-kb genome contains ~373 protein-encoding genes and 11 tRNA genes. The predicted gene products of ~50% of these genes resemble proteins of known function, including many that are unexpected for a virus, e.g., ornithine decarboxylase, hyaluronan synthase, GDP-D-mannose 4,6 dehydratase, and a potassium ion channel protein. In addition to their large genome size, the chlorella viruses have other features that distinguish them from most viruses. These features include: (a) The viruses encode multiple …

A Protein Kinase From Colletotrichum Trifolii Is Induced By Plant Cutin And Is Required For Appressorium Formation, M. B. Dickman, Y.-S. Ha, Z. Yang, B. J. Adams, C. Huang

Department of Plant Pathology: Faculty Publications

When certain phytopathogenic fungi contact plant surfaces, specialized infection structures (appressoria) are produced that facilitate penetration of the plant external barrier; the cuticle. Recognition of this hydrophobic host surface must be sensed by the fungus, initiating the appropriate signaling pathway or pathways for pathogenic development. Using polymerase chain reaction and primers designed from mammalian protein kinase C sequences (PKC), we have isolated, cloned, and characterized a protein kinase from Colletotrichum trifolii, causal agent of alfalfa anthracnose. Though sequence analysis indicated conserved sequences in mammalian PKC genes, we were unable to induce activity of the fungal protein using known activators …

The Pseudomonas Syringae Type Iii-Secreted Protein Hopptod2 Possesses Protein Tyrosine Phosphatase Activity And Suppresses Programmed Cell Death In Plants, Avelina Espinosa, Ming Guo, Vincent C. Tam, Zheng Qing Fu, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The bacterial plant pathogen Pseudomonas syringae possesses a type III protein secretion system that delivers many virulence proteins into plant cells. A subset of these proteins (called Avr proteins) is recognized by the plant’s innate immune system and triggers defences. One defence-associated response is the hypersensitive response (HR), a programmed cell death (PCD) of plant tissue. We have previously identified HopPtoD2 as a type III secreted protein from P. s. pv. tomato DC3000. Sequence analysis revealed that an N-terminal domain shared homology with Avr- PphD and a C-terminal domain was similar to protein tyrosine phosphatases (PTPs). We demonstrated that purified …

Characterization Of Wound-Inducible Genes Encoding Enzymes For Terpenoid Biosynthesis In Medicago Truncatula, Mandy M. Cox

Inquiry: The University of Arkansas Undergraduate Research Journal

In addition to having numerous applications and pharmaceuticals, terpenoids are an important class of defensive compounds that can accumulate in plants after pathogen infection or injury by insects. Sequences of DNA encoding putative terpene synthases and an oxidosqualene synthase, isolated from insect-damaged Medicago truncatula leaves, were selected from an expressed sequence tag (EST) data base. The cDNA clones were used as radio-labeled probes to analyze gene expression in leaves treated with factors known to trigger a defense response in plants. Transcript levels for all of the genes examined increased in response to artificial wounding, insect herbivory, and methyl jasmonate (meJA) …

Chitosanase May Enhance Anti-Fungal Defense Responses In Transgenic Tobacco, Bill L. Hendrix

Inquiry: The University of Arkansas Undergraduate Research Journal

Fungicides are expensive, dangerous, and can be harmful to the environment, but they are often necessary for profitable farming operations. New technologies may soon allow farmers to replace these chemicals with genetically engineered plants producing antifungal enzymes that degrade fungal cell walls. To explore this option, a Paenbacillus chitosanase gene was cloned, sequenced, and modified for plant expression. The modified gene was delivered to tobacco (Nicotiana tabacum L cv. Xanthine) leaf disks via Agrobacterium tumenfaciens-mediated transformation. The putative GMOs were tested for transgene integration, transcription, and translation. Confirmed transformants were then screened for enhanced responses to a Rhizoctonia solani cell …

The Role Of Natural Calcium Oxalate Crystals In Plant Defense Against Chewing Insects, Sarah J. Doege

Inquiry: The University of Arkansas Undergraduate Research Journal

Calcium oxalate is the most abundant insoluble mineral found in plants and it is common among many plant families. Calcium oxalate crystals in plants can appear as irregular rectangles, spiked balls, or needles. The formidable appearance of these crystals has lead to speculation that they might serve as a form of pest control by deterring chewing insects. We utilized mutant plant lines to assess the effects of plant calcium oxalate crystals on the survival and feeding habits of chewing insects. We have taken advantage of calcium oxalate-deficient (cod) mutants of the barrel medic, Medicago truncatula Calcium oxalate crystals accumulate in …

Are Chlorella Viruses A Rich Source Of Ion Channel Genes?, Ming Kang, Anna Moroni, Sabrina Gazzarrini, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Plaque-forming dsDNA (>330 kb) viruses that infect certain unicellular, eukaryotic chlorella-like green algae contain ~375 protein-encoding genes. These proteins include a 94 amino acid K+ channel protein, called Kcv, as well as two putative ligand-gated ion channels. The viruses also encode other proteins that could be involved in the assembly and/ or function of ion channels, including protein kinases and a phosphatase, polyamine biosynthetic enzymes and histamine decarboxylase.

Fungal Biology And Agriculture: Revisiting The Field, O. Yarden, D. J. Ebbole, S. Freeman, R. J. Rodriguez, M. B. Dickman

Department of Plant Pathology: Faculty Publications

Plant pathology has made significant progress over the years, a process that involved overcoming a variety of conceptual and technological hurdles. Descriptive mycology and the advent of chemical plant-disease management have been followed by biochemical and physiological studies of fungi and their hosts. The later establishment of biochemical genetics along with the introduction of DNA-mediated transformation have set the stage for dissection of gene function and advances in our understanding of fungal cell biology and plant–fungus interactions. Currently, with the advent of high-throughput technologies, we have the capacity to acquire vast data sets that have direct relevance to the numerous …

Transcription Strategy In A Closterovirus: A Novel 5'-Proximal Controller Element Of Citrus Tristeza Virus Produces 5'- And 3'-Terminal Subgenomic Rnas And Differs From 3' Open Reading Frame Controller Elements, Siddarame Gowda, Maria A. Ayllon, Satyanarayana Tatineni, Moshe Bar-Joseph, William O. Dawson

Department of Plant Pathology: Faculty Publications

Citrus tristeza virus (CTV) produces more than thirty 3'- or 5'-terminal subgenomic RNAs (sgRNAs) that accumulate to various extents during replication in protoplasts and plants. Among the most unusual species are two abundant populations of small 5'-terminal sgRNAs of approximately 800 nucleotides (nt) termed low-molecular-weight tristeza (LMT1 and LMT2) RNAs. Remarkably, CTV replicons with all 10 3' genes deleted produce only the larger LMT1 RNAs. These 5'-terminal positive-sense sgRNAs do not have corresponding negative strands and were hypothesized to be produced by premature termination during plus-strand genomic RNA synthesis. We characterized a cis-acting element that controls the production …

Evaluation Of Resistance Screening Methods For Sclerotinia Stem Rot Of Soybean And Dry Bean, Linda S. Kull, Tri D. Vuong, Kris S. Powers, Kent M. Eskridge, James R. Steadman, Glen L. Hartman

Department of Plant Pathology: Faculty Publications

Three methods to identify levels of resistance to Sclerotinia sclerotiorum in soybean (Glycine max) and dry bean (Phaseolus vulgaris) were compared using multiple data analyses. The three methods were mycelial plug inoculations of cotyledons, cut stems, and detached leaves. Six S. sclerotiorum isolates of known relative aggressiveness were inoculated on each of three soybean and dry bean cultivars with varied response to S. sclerotiorum. For soybean, all three inoculation methods accurately identified isolate aggressiveness irrespective of cultivar, but identification of susceptible and partially resistant soybean cultivars was influenced by isolate. For dry bean, the cotyledon and cut …

Relationship Between Secondary Metabolism And Fungal Development, Ana M. Calvo, Richard A. Wilson, Jin Woo Bok, Nancy P. Keller

Fungal Molecular Plant-Microbe Interactions

Details findings related to the relationship between secondary metabolism and fungal development.

Uses Of Antimicrobials In Plant Agriculture, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

Bacterial diseases of plants are less prevalent than diseases caused by fungi and viruses. Antimicrobials for prophylactic treatment of bacterial diseases of plants are limited in availability, use, and efficacy, and therapeutic use is largely ineffective. Most applications are by spray treatments in orchards. Monitoring and surveillance for drug resistance are not routinely done. In the United States, data on use of antimicrobials for treatment of bacterial diseases of plants are limited to streptomycin and oxytetracycline. Resistance to streptomycin has become widespread among bacterial phytopathogens; no resistance among these bacteria has yet been reported for oxytetracycline. No human health effects …

Isolation And Characterization Of Endophytic Colonizing Bacteria From Agronomic Crops And Prairie Plants, Denise K. Zinniel, Patricia A. Lambrecht, N. Beth Harris, Zhengyu Feng, Daniel Kuczmarski, Phyllis Higley, Carol A. Ishimaru, Alahari Arunakumari, Raul G. Barletta, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

Endophytic bacteria reside within plant hosts without causing disease symptoms. In this study, 853 endophytic strains were isolated from aerial tissues of four agronomic crop species and 27 prairie plant species. We determined several phenotypic properties and found approximately equal numbers of gram-negative and gram-positive isolates. In a greenhouse study, 28 of 86 prairie plant endophytes were found to colonize their original hosts at 42 days postinoculation at levels of 3.5 to 7.7 log₁₀ CFU/g (fresh weight). More comprehensive colonization studies were conducted with 373 corn and sorghum endophytes. In growth room studies, none of the isolates displayed pathogenicity, …

Proceedings Of The 27th Annual Meeting, Southern Soybean Disease Workers (April 6-8, 2000, Fort Walton Beach Florida), John S. Russin, Stephen R. Koenning, Raymond W. Schneider, Peggy S. King

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern United States Soybean Disease Loss Estimate for 1999. Compiled by SR Koenning

Treasury report. Peggy S King

Invited presentation

Bean Pod Mottle: A Soybean Disease on the Rise in the New Millennium. SA Ghabrial

Contributed papers

Response of Selected Mid-South Soybean Varieties to the Reniform Nematode. GW Lawrence, KS McLean, and SM Baird

Field Response of Soybean Cultivars to the Reniform Nematode Rotylenchulus reniformis. PS King, DB Weaver, and R Rodriguez-Kabana

Approaches to Race Determination in Soybean Cyst Nematode. AJ Palmateer, ME Schmidt, SR Stetina, and JS Russin

Nematological Survey of Selected Soybean and Cotton Fields in …

The Shca Protein Is A Molecular Chaperone That Assists In The Secretion Of The Hoppsya Effector From The Type Iii (Hrp) Protein Secretion System Of Pseudomonas Syringae, Karin V. Van Dijk, Vincent C. Tam, Angela R. Records, Tanja Petnicki-Ocwieja, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

Pseudomonas syringae uses a type III protein secretion system encoded by the Hrp pathogenicity island (Pai) to translocate effector proteins into plant cells. One of these effector proteins is HopPsyA. A small open reading frame (ORF), named shcA, precedes the hopPsyA gene in the Hrp Pai of P. s. syringae 61. The predicted amino acid sequence of shcA shares general characteristics with chaperones used in type III protein secretion systems of animal pathogens. A functionally non-polar deletion of shcA in P. s. syringae 61 resulted in the loss of detectable HopPsyA in supernatant fractions, consistent with ShcA acting as a …

Crop Updates 2002 - Geraldton, Steve Penny Jr, Robin Wilson, Iian Barclay, Robyn Mclean, Robert Loughman, Jenny Garlinge, Bill Lambe, Neil Venn, Peter Clarke, Wal Anderson, Raffaele De Cima, James Bee, Darshan Sharma, Sheena Lyon, Melaine Kupsch, Mohammad Amjad, Pam Burgess, Veronika Reck, Brenda Shackley, Ray Tugwell, Bindi Webb, Robert Park, Daya Patabendige, Phil Michael, Debbie Thackray, Jenny Hawkes, Roger Jones, David Stephens, Bob French, Laurie Wahlsten, Glen Riethmuller, M. Bolland, P. White, T. Khan, Kevin Walden, Ravjit Khangura, Martin J. Barbetti, Graham Walton, Françoise Berlandier, Paul Carmody, Christiaan Valentine, Roy Latter, Keith Devenish, Bill Bowden, Michael O'Connell, Chris Gazey, David Gartner, Caroline Peek, David Rogers, Greg Shea, George Yan, Alexandra Douglas, Sally Peltzer, Paul Matson, David Minkey, Clinton Revell, Giles Glasson, Dean Thomas, Aik Cheam, Siew Lee, Mike Clarke, Peter Newman, Glenn Adam

Crop Updates

This session covers twenty seven papers from different authors:

1. Taking the Why out of Wyalkatchem – the new widely adapted wheat variety, Steve Penny Jr, Department of Agriculture

2. Future wheat varieties, Robin Wilson, Iain Barclay,Robyn McLean, Robert Loughman, Jenny Garlinge, Bill Lambe, Neil Venn and Peter Clarke

Department of Agriculture

3. Maximising wheat variety performance through agronomic management, Wal Anderson, Raffaele Del Cima, James Bee, Darshan Sharma, Sheena Lyon, Melaine Kupsch, Mohammad Amjad, Pam Burgess, Veronika Reck, Brenda Shackley, Ray Tugwell, Bindi Webb and Steve Penny Jr

Department of Agriculture

4. Cereal rust update 2002 – …

Phylogenetic Relationships, Strain Diversity And Biogeography Of Tritimoviruses, Frank Rabenstein, Dallas L. Seifers, Jorg Schubert, Roy C. French, Drake C. Stenger

Department of Plant Pathology: Faculty Publications

North American and Eurasian isolates of Wheat streak mosaic virus (WSMV; genus Tritimovirus) and Oat necrotic mottle virus (ONMV; genus Rymovirus) were examined. Nine WSMV isolates differentially infected oat, barley, inbred maize line SDp2 and sorghum line KS56. The WSMV isolates clustered into groups based on phylogenetic analyses of the capsid protein (CP) cistron and flanking regions. WSMV isolates from the United States (US) and Turkey were closely related, suggesting recent movement between continents. Although more divergent, WSMV from Iran (WSMV-I) also shared a most recent common ancestor with the US and Turkish isolates. Another group of WSMV …

Crop Updates 2002 - Lupins, Amelia Mclarty, Bob French, Laurie Wahlsten, Martin Harries, Paul Blackwell, Mike Collins, Bill Crabtree, Geoff Fosbery, Angie Roe, Matt Beckett, Abul Hashem, Nerys Wilkins, Geoff Thomas, Ken Adcock, Debbie Thackray, Jenny Hawkes, Roger Jones, Brenda Coutts, Manisha Shankar, Mark Sweetingham, Bevan Buirchell, Nichole Burges, Dominie Wright, Susan J. Barker, Jairo A. Palta, Neil C. Turner, Matt Evans, Brett Glencross, John Curnow, Wayne Hawkins, Jon C. Clements

Crop Updates

This session covers twenty four papers from different authors:

LUPIN INDUSTRY ISSUES AND RESEARCH DIRECTIONS

ACKNOWLEDGMENTS Amelia McLarty LUPIN CONVENOR DEPARTMENT OF AGRICULTURE

VARIETIES

1. Evaluation of lupinus mutabilis in Western Australia, Bob French, Laurie Wahlsten and Martin Harries, Department of Agriculture

2. Adaption of restricted-branching lupins in short-growing season environments, Bob French, Laurie Wahlsten, Department of Agriculture

ESTABLISHMENT

3. Moisture delving for better lupin establishment, Dr Paul Blackwell, Department of Agriculture

4. Lupins, tramlines, 600mm rows, rolling and shield spraying … a good result in a dry season! Paul Blackwell and Mike Collins, Department of Agriculture

5. Lupin …

Crop Updates 2002 - Oilseeds, David Eksteen, K. Neil Harker, George W. Clayton, Keith Downey, Keith Alcock, Kevin Walden, Beven Addison, Peter Carlton, Kevin Morthorpe, Stephen Addenbrooke, Alex Ford, S. Hasan Zaheer, G. Walton, Imma Farré, Paul Carmody, J. A. Fortescue, D. W. Turner, B. Tan, Margaret C. Campbell, Ian Pritchard, Richard W. Bell, K. Frost, Mike Wong, Ross Brennan, Roger Jones, Jenny Hawkes, Debbie Thackray, Moin U. Salam, Ravjit K. Khangura, Art J. Diggle, Martin Barbetti, Phil Michael, Françoise Berlandier, Christiaan Valentine, Greg Shea, Glen Riethmuller, Rafiul Alam, Greg Hamilton, Jo Hawksley, Patrick Smith, Paul Neve, Felicity Flugge, Amir Abadi, Stephen Powles, Brett Glencross, John Curnow, Wayne Hawkins

Crop Updates

This session covers twenty seven papers from different authors:

1. Forward and acknowledgements, Dave Eksteen, ACTING MANAGER OILSEEDS PRODUCTIVITY AND INDUSTRY DEVELOPMENT Department of Agriculture

PLENARY SESSION

2. GMO canola - Track record in Canada, K. Neil Harker and George W. Clayton,Agriculture and Agri-Food Canada, Lacombe Research Centre, Lacombe, Alberta, R. Keith Downey, Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, Saskatchewan

3. GMO canola – Prospects in Western Australia farming systems, Keith Alcock, Crop Improvement Institute, Department of Agriculture

4. Diamondback moth (DBM) in canola, Kevin Walden, Department of Agriculture

CANOLA AGRONOMY

5. Getting the best out of …

Crop Updates 2002 - Pulse Research And Industry Development In Western Australia, M. Harries, R. French, I. Pritchard, N. Brandon, N. Runciman, S. White, M. Seymour, P. White, J. Howieson, R. Yates, H. Dhammu, T. Piper, T. Khan, K. Regan, N. Suizu, D. Diepeveen, C. Veitch, J. Palta, A. Nandwal, N. Turner, H. Clarke, G. Dwyer, H. Loo, K. Siddique, M. Bellgard, M. Jones, Glen Riethmuller, B. Mcleod, R. Shackles, P. Smith, C. Francis, M. Bolland, M. Materne, J. Clements, C. Hanbury, R. Matic, M. Tate, B. Hughes, B. French, P. Rees, J. Galloway, M. Salam, A. Diggle, N. Burges, D. Wright, J. Hawkes, D. Thackray, R. Jones, O. Edwards, J. Ridsdill-Smith, R. Horbury, F. Berlandier, O. Byrne, D. Hardie, H. Sharma, M. De Sousa Majer, R. Roush, R. Morton, T. Higgins

Crop Updates

This session covers seventy one papers from different authors:

1. 2001 PULSE INDUSTRY HIGHLIGHTS

CONTRIBUTORS

BACKGROUND

2001 REGIONAL ROUNDUP

2. Northern Agricultural Region, M. Harries, Department of Agriculture

3. Central Agricultural Region, R. French and I. Pritchard, Department of Agriculture

4. Great Southern and Lakes, N. Brandon, N. Runciman and S. White, Department of Agriculture

5. Esperance Mallee, M. Seymour, Department of Agriculture

PULSE PRODUCTION AGRONOMY AND GENETIC IMPROVEMENT

6. Faba bean, P. White, Department of Agriculture

7. Germplasm evaluation, P. White, M. Seymour and M. Harries, Department of Agriculture

8. Variety evaluation, P. White, M. Harries, N. Brandon …

Crop Updates 2002 - Farming Systems, Peter Metcalfe, Mike Ewing, Roy Latta, Keith Devenish, Diana Fedorenko, Clayton Butterly, Chantelle Butterly, Kim Diamond, Neil Diamond, Stuart Mcalpine, Bill Bowden, Jessica Johns, Terry Piper, David Bowran, Robert Beard, Phil Ward, Dominie Wright, Nichole Burges, Roger Jones, Danae Harman, Greg Shea, George Yan, David Tennant, David Hall, Anyou Liu, Clinton Revell, Candy Hudson, Andrew Blake, Natalie Lauritsen, John Fosu-Nyarko, Roger Jones, Lisa Smith, Mike Jones, Geoff Dwyer, Bill Bowden, Michael O'Connell, Chris Gazey, David Gartner, Amanda Miller, Richard W. Bell, K. Frost, Mike Wong, Ross Brennan, N. J. Blake, G. Mconnell, D. Patabendige, N. Venn, Derk Bakker, Greg Hamilton, Dave Houlbrooke, Cliff Spann, Paul Blackwell, Bindi Webb, G. Lyle, K. Wittwer, Perry Dolling, Senthold Asseng, Ian Fillery, Michael Robertson, Caroline Peek, David Rogers, Peter Portman, Jeff Russell, Greg Shea, Ben Henderson, Ross Kingwell, Tresslyn Walmsley, Jean Galloway, Debbie Thackray, Moin Salam, Art Diggle, William J. Macleod, Jenny Hawkes

Crop Updates

This session covers forty one papers from different authors:

INTRODUCTION

1. Future Farming Systems session for Crop Updates 2002 Peter Metcalf, FARMING SYSTEMS SUBPROGRAM MANAGER GRAINS PROGRAM Department of Agriculture

2. Perennial pastures in annual cropping systems: Lucerne and beyond, the ‘Big Picture’, Mike Ewing, Deputy CEO CRC for Plant-based Management of Dryland Salinity, Department of Agriculture

3. Perennial pastures in annual cropping systems: lucerne and beyond, Roy Latta and Keith Devenish, Department of Agriculture

4. Establishing Lucerne with a cover crop, Diana Fedorenko¹, Clayton Butterly¹, Chantelle Butterly¹, Kim and Neil Diamond^{2 …}

Genomewide Identification Of Pseudomonas Syringae Pv.Tomato Dc3000 Promoters Controlled By The Hrpl Alternative Sigma Factor, Derrick E. Fouts, Robert B. Abramovitch, James R. Alfano, Angela M. Baldo, C. Robin Buell, Samuel Cartinhour, Arn K. Chatterjee, Mark D'Ascenzo, Michelle L. Gwinn, Sondra G. Lazarowitz, Na-Chun Lin, Gregory B. Martin, Amos H. Rehm, David J. Schneider, Karin V. Van Dijk, Xiaoyan Tang, Alan Collmer

Center for Plant Science Innovation: Faculty Publications

The ability of Pseudomonas syringae pv. tomato DC3000 to parasitize tomato and Arabidopsis thaliana depends on genes activated by the HrpL alternative sigma factor. To support various functional genomic analyses of DC3000, and specifically, to identify genes involved in pathogenesis, we developed a draft sequence of DC3000 and used an iterative process involving computational and gene expression techniques to identify virulence-implicated genes downstream of HrpLresponsive promoters. Hypersensitive response and pathogenicity (Hrp) promoters are known to control genes encoding the Hrp (type III protein secretion) machinery and a few type III effector proteins in DC3000. This process involved (i) identification of …

Functional Characterization And Localization Of The Aspergillus Nidulan Formin Sepa, Kathryn E. Sharpless, Steven D. Harris

Department of Plant Pathology: Faculty Publications

Formins are a family of multidomain scaffold proteins involved in actin-dependent morphogenetic events. In Aspergillus nidulans, the formin SEPA participates in two actin-mediated processes, septum formation and polarized growth. In this study, we use a new null mutant to demonstrate that SEPA is required for the formation of actin rings at septation sites. In addition, we find that a functional SEPA::GFP fusion protein localizes simultaneously to septation sites and hyphal tips, and that SEPA colocalizes with actin at each site. Using live imaging, we show that SEPA localization at septation sites and hyphal tips is dynamic. Notably, at septation …