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Articles 12931 - 12960 of 13586
Full-Text Articles in Entire DC Network
Genetic Markers In Plant Evolutionary Ecology, Mitchell B. Cruzan
Genetic Markers In Plant Evolutionary Ecology, Mitchell B. Cruzan
Biology Faculty Publications and Presentations
Genetic markers have provided plant ecologists with a method of assessing levels of genetic relatedness among individuals and populations. In recent years a number of techniques based on DNA sequence variation have been developed to complement allozyme methods that are already widely used. Some of these new markers are more variable than protein-based markers, allowing more precise estimates of genetic differences among individuals and populations. Other DNA-based markers are based on organelle genomes that are inherited uniparentally. These cytoplasmic markers can provide a method for assessing the separate effects of seed and pollen dispersal on gene flow within and among …
Peanut Yellow Spot Virus Is A Member Of A New Serogroup Of Tospovirus Genus Based On Small (S) Rna Sequence And Organization, Satyanarayana Tatineni, S. Gowda, K. Lakshminarayana Reddy, S. E. Mitchell, W. O. Dawson, D. V. R. Reddy
Peanut Yellow Spot Virus Is A Member Of A New Serogroup Of Tospovirus Genus Based On Small (S) Rna Sequence And Organization, Satyanarayana Tatineni, S. Gowda, K. Lakshminarayana Reddy, S. E. Mitchell, W. O. Dawson, D. V. R. Reddy
Department of Plant Pathology: Faculty Publications
Peanut yellow spot virus (PYSV) represents a distinct tospovirus species based on serology and nucleic acid hybridization. The sequence of the S RNA was 2 970 nucleotides with 22 nucleotide long inverted repeats (with three mismatches) at the termini. The coding was ambisense with a long open reading frame (ORF) in each strand. The 5'-large ORF (1 440 nucleotides in the viral sense (v) strand) encoded a protein with a predicted size of 53.2 kDa that was identified as the nonstructural (NSs) protein based on 16–21% sequence identity and 42– 48% sequence similarity with other tospoviruses. A 3' ORF (741 …
Growth Traits Among Sorghum Genotypes In Response To Aluminum, J. H. Bernal, R. B. Clark
Growth Traits Among Sorghum Genotypes In Response To Aluminum, J. H. Bernal, R. B. Clark
Department of Agronomy and Horticulture: Faculty Publications
Aluminum (Al) has many detrimental effects on plant growth, and shoots and roots are normally affected differently. A study was conducted to determine differences among sorghum [Sorghum bicolor (L.) Moench] genotypes with broad genetic backgrounds for growth traits of plants grown at 0,200,400,600, and 800 uM Al in nutrient solutions (pH 4.0). Genotypes were categorized into "Al-sensitive", "intermediate Al-tolerant", "Al-tolerant", and SC 283 (an Al-tolerant standard). As Al increased, shoot and root dry matter (DM), net main axis root length (NMARL), and total root length (TRL) became lower than controls (0 Al). Aluminum toxicity and/or nutrient deficiency symptoms become …
Population Biology Of Intraspecific Polyploidy In Grasses, Kathleen H. Keeler
Population Biology Of Intraspecific Polyploidy In Grasses, Kathleen H. Keeler
School of Biological Sciences: Faculty Publications
Polyploidy is the duplication of an entire nuclear genome, whether diploid or higher level (Stebbins, 1971; Thompson and Lumaret, 1992) and a frequent occurrence in plants. Stebbins (1971) estimated that 30-35% of flowering plant species are polyploid, and that many more had a polyploid event in their evolutionary history, including all members of such important families as the Magnoliaceae, Salicaceae, and Ericaceae. Goldblatt (1980) estimated 55%, but probably up to 75%, of monocotyledons had at least one polyploid event in their history, using the criterion that if the species has a base number higher than n = 13 it is …
A Solid Phase Extraction System For The Isolation Of The Germination Cue In Smoky Water When Subjected To The Light Sensitive Lettuce Lactuca Sativa V. Grand Rapids, Troy David Adriansz
A Solid Phase Extraction System For The Isolation Of The Germination Cue In Smoky Water When Subjected To The Light Sensitive Lettuce Lactuca Sativa V. Grand Rapids, Troy David Adriansz
Theses : Honours
The search for the chemical or set of chemicals that act as a germination stimulant in Lactuca sativa v. Grand rapids (a variety of lettuce seed) was the underlying basis of this project. Through the development of a solid phase extraction (SPE) system different fractions of smoky water were tested for their effects on germination enhancement. The project targeted streamlining the amount of solvents as well as time used in past research on smoky water. Previously, several steps involving high perfo1mance liquid chromatography (HPLC), thin layer chromatography (TLC) and Gas Chromatography (GC) were employed. This project used SPE cartridges to …
Seedling Development In Species Of Chamaesyce (Euphorbiaceae) With Erect Growth Habits, W. John Hayden, Olga Troyanskaya
Seedling Development In Species Of Chamaesyce (Euphorbiaceae) With Erect Growth Habits, W. John Hayden, Olga Troyanskaya
Biology Faculty Publications
Seedling development is described for Chamaesyce hirta, C. hypericifolia, and C. mesembrianthemifolia as discerned by light microscopy and scanning electron microscopy. Although these species ultimately develop erect to ascending growth habits, epicotyl development is limited to the production of a single pair ofleaves located immediately superjacent to and decussate with the cotyledons. The shoot system develops from one or more buds located in the axils of the cotyledons. In all respects, seedling ontogeny is very similar to that of previously studied prostrate species of Chamaesyce. Evidence from seedling ontogeny thus contradicts a hypothesis concerning homologies of plant …
Identification Of The Wheat Curl Mite As The Vector Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen
Identification Of The Wheat Curl Mite As The Vector Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen
Department of Plant Pathology: Faculty Publications
Wheat with virus-like symptoms (extracts containing a 33-kDa protein in sodium dodecyl sulfate- polyacrylamide gel electrophoresis, negative in enzyme-linked immunosorbent assay to wheat streak mosaic virus, and not infectious in a backassay to other wheat) reacted positively to antiserum made against a protein purified from symptomatic corn infected with the High Plains virus (HPV), indicating a serological relationship between the corn and wheat pathogens. The wheat curl mite (WCM, Aceria tosichella Keifer) was identified as the vector of the virus and caused persistent infection of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) in greenhouse experiments. The …
Comparison Of Avrd Alleles From Pseudomonas Syringae Pv. Glycinea, Lisa Wolfson Keith, Carol Boyd, Noel T. Keen, J. E. Patridge
Comparison Of Avrd Alleles From Pseudomonas Syringae Pv. Glycinea, Lisa Wolfson Keith, Carol Boyd, Noel T. Keen, J. E. Patridge
Department of Plant Pathology: Faculty Publications
Avirulence gene D alleles resided on indigenous plasmids in races 0, 2, 3, 4, 5, and 6 of Pseudomonas syringae pv. glycinea (Psg), but the allele in race 1 appeared to be chromosomal. These were all nonfunctional avirulence genes because they neither induced the avirulence phenotype on Rpg4 soybean cultivars nor directed the production of syringolide elicitors when expressed in Escherichia coli cells. The predicted proteins encoded by the seven Psg avrD genes were very similar to that of a functional class II allele from P. syringae pv. phaseolicola G50 race 2, but contained mutations collectively …
Revision Of The Cerrado Hemicryptophytic Chamaesyce Of Boissier's "Pleiadeniae" (Euphorbiaceae), Mark P. Simmons, W. John Hayden
Revision Of The Cerrado Hemicryptophytic Chamaesyce Of Boissier's "Pleiadeniae" (Euphorbiaceae), Mark P. Simmons, W. John Hayden
Biology Faculty Publications
The species of Chamaesyce classified by Boissier as the "Pleiadeniae'" are revised in light of presently available collections. Six species are accepted and new combinations are proposed for C. nana, C. setosa, C. tamanduana, and C. viscoides. Although these herbaceous perennials of cerrado vegetation of Brazil, northern Argentina, and adjacent countries are distinctive ecologically and geographically, cladistic analysis does not support their recognition as a monophyletic group.
Genetic Variability Among Maize Inbred Lines For Resistance To The High Plains Virus–Wheat Streak Mosaic Virus Complex, A. Marçon, S. M. Kaeppler, S. G. Jensen
Genetic Variability Among Maize Inbred Lines For Resistance To The High Plains Virus–Wheat Streak Mosaic Virus Complex, A. Marçon, S. M. Kaeppler, S. G. Jensen
Department of Plant Pathology: Faculty Publications
High plains virus (HPV) is a pathogen that causes a severe disease, especially in susceptible corn genotypes. The virus is transmitted by the eriophyid mite Aceria tosichella, which also transmits wheat streak mosaic virus (WSMV). This often results in a mixed infection by these two viruses. Genetic variability for resistance to the HPV exists among maize inbred lines but has not been characterized. The disease reaction of 30 maize inbred lines to the mixed infection and to WSMV alone was characterized in this study. Evaluation was based on symptom expression and virus titer (HPV and WSMV), as measured by …
Between A Rock And A Hard Place: Opuntia Fragilis (Nutt.) Haw. (Cactaceae) In Stearns County, Minnesota, Eric Ribbens, Jeremy Myrom
Between A Rock And A Hard Place: Opuntia Fragilis (Nutt.) Haw. (Cactaceae) In Stearns County, Minnesota, Eric Ribbens, Jeremy Myrom
Journal of the Minnesota Academy of Science
We surveyed the distribution of Opuntia fragilis ( Nutt.) Haw. in Steam County, MN, where it is restricted to a few rock outcrops. To compare the populations within three sites, we mapped clusters of pads and measured each individual pad within each cluster. Pads tended to be located in a narrow band of shallow soil between the bare rock and the angiosperm-dominated plant communities growing on adjacent deeper soils. Significant differences existed between the three populations in pad widths, average pad cluster sizes, and pad appearances. Although several pads flowered, none of the flower set seed, indicating that persistence of …
A Biosystematic Survey Of Sierra Lodgepole Pine (Pinus Contorta Var. Murrayana [Critchfield]) Populations In The Transverse And Peninsular Ranges Of Southern California, Richard Gobin Everett
A Biosystematic Survey Of Sierra Lodgepole Pine (Pinus Contorta Var. Murrayana [Critchfield]) Populations In The Transverse And Peninsular Ranges Of Southern California, Richard Gobin Everett
Theses Digitization Project
No abstract provided.
Molecular And Functional Characterization Of A Novel Low-Affinity Cation Transporter (Lct1) In Higher Plants, Daniel P. Schachtman, Raman Kumar, Julian I. Schroeder, Ellen L. Marsh
Molecular And Functional Characterization Of A Novel Low-Affinity Cation Transporter (Lct1) In Higher Plants, Daniel P. Schachtman, Raman Kumar, Julian I. Schroeder, Ellen L. Marsh
Department of Agronomy and Horticulture: Faculty Publications
The transport of cations across membranes in higher plants plays an essential role in many physiological processes including mineral nutrition, cell expansion, and the transduction of environmental signals. In higher plants the coordinated expression of transport mechanisms is essential for specialized cellular processes and for adaptation to variable environmental conditions. To understand the molecular basis of cation transport in plant roots, a Triticum aestivum cDNA library was used to complement a yeast mutant deficient in potassium (K+) uptake. Two genes were cloned that complemented the mutant: HKT1 and a novel cDNA described in this report encoding a cation …
Comparative Salt And Waterlogging Tolerance Of An Introduced Grass (Agropyron Elongatum [Host] P. Beauv.) And Two Herbaceous Australian Species (Podolepis Gracilis [Lehm.] And Danthonia Caespitosa Gaud.), Bryan William Duff
Theses : Honours
Increasing land degradation is recognised as an immediate worldwide threat. Human induced soil salinity is probably the major cause of land abandonment, through its adverse effects on plant growth. Salinity is a major focus of environmental researchers because it is recognised that counter-strategies can potentially reclaim both artificially degraded lands and intrinsically saline areas. Currently, strategies to combat salinity require that land use is changed, since restoration to a past use is usually economically impractical or impossible. Biological strategies show most promise. Revegetation of degraded soils with hardy plant species has met with considerable success, and shows promise for the …
Soil Temperature And Fumigation Effects On Plant Phosphorus Uptake And Related Microbial Properties, Dennis L. Mccallister, L. A. Jawson, M. D. Jawson
Soil Temperature And Fumigation Effects On Plant Phosphorus Uptake And Related Microbial Properties, Dennis L. Mccallister, L. A. Jawson, M. D. Jawson
Department of Agronomy and Horticulture: Faculty Publications
Early season problems with growth of corn (Zea mays L.) under cool, wet conditions prompted a study of the effects of soil and environmental conditions on mineralization and plant uptake of phosphorus (P). Our objective was to determine the effect of soil test P, temperature, and soil fumigation on soil P availability and uptake during early corn growth. Corn was grown in growth chambers at temperatures of 14°C or 25°C. Soils were a high-P Hastings silty clay loam (fine, montmorillonitic, mesic Udic Argiustoll) and a low-P Sharpsburg clay loam (fine, montmorillonitic, mesic Typic Argiudoll). Plants grew for up to …
Evolutionary Implications Of Meiotic Chromosome Behavior, Reproductive Biology, And Hybridization In 6x And 9x Cytotypes Of Andropogon Gerardii (Poaceae), Guillermo A. Norrmann, Camilo L. Quarín, Kathleen H. Keeler
Evolutionary Implications Of Meiotic Chromosome Behavior, Reproductive Biology, And Hybridization In 6x And 9x Cytotypes Of Andropogon Gerardii (Poaceae), Guillermo A. Norrmann, Camilo L. Quarín, Kathleen H. Keeler
School of Biological Sciences: Faculty Publications
Andropogon gerardii, big bluestem, has 60 and 90 chromosome cytotypes. Meiosis in the hexaploid was shown to be regular, although some secondary associations of bivalents form. Meiosis in the enneaploid (2n = 9x = 90) is irregular, leading to most gametes having unbalanced chromosome complements. Both cytotypes show considerable self-incompatibility. Cytotypes crossed freely, forming a variety of fertile euploids and aneuploids. Indistinguishable exomorphology, intermixing in natural populations, and compatibility suggest that A. gerardii is best understood as a cytotypically complex single species.
Notes: Forage Yield, Quality, And Fertility Of Sorghum X Sudan Grass Hybrids In A1 And A3 Cytoplasm, Jeffrey F. Pedersen, J. J. Toy
Notes: Forage Yield, Quality, And Fertility Of Sorghum X Sudan Grass Hybrids In A1 And A3 Cytoplasm, Jeffrey F. Pedersen, J. J. Toy
Department of Agronomy and Horticulture: Faculty Publications
Currently, no information is available comparing the agronomic performance of A1 and A3 cytoplasms in sorghum x sudangrass [Sorghumbi color (L.) Moench] hybrids. The objectives of this study were to compare the effects of A1 and A3 cytoplasm on the maturity, fertility, height, forage yield, and forage quality of sorghum × sudangrass hybrids. In 1989, pollen from a bulk of eight sudangrass populations was used to pollinate four sorghum lines that had been male-sterilized in both A1 and A3 cytoplasm. Hybrids were grown at the Univ. of Nebraska Field Laboratory Ithaca, NE, in 1990 and 1991, in a split-plot …
Evaluation Of The Success Of Constructed Wetlands In The Cave Run Lake Watershed, April Diane Haight
Evaluation Of The Success Of Constructed Wetlands In The Cave Run Lake Watershed, April Diane Haight
Morehead State Theses and Dissertations
A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by April Diane Haight on December 10, 1996.
Cross-Polarized Magic-Angle Spinning 13c Nuclear Magnetic Resonance Spectroscopic Characterization Of Soil Organic Matter Relative To Culturable Bacterial Species Composition And Sustained Biological Control Of Pythium Root Rot, Michael J. Boehm, Tianyun Wu, Alex G. Stone, Bart Kraakman, Donna A. Iannotti, G. Edwin Wilson, Laurence V. Madden, Harry A.J. Hoitink
Cross-Polarized Magic-Angle Spinning 13c Nuclear Magnetic Resonance Spectroscopic Characterization Of Soil Organic Matter Relative To Culturable Bacterial Species Composition And Sustained Biological Control Of Pythium Root Rot, Michael J. Boehm, Tianyun Wu, Alex G. Stone, Bart Kraakman, Donna A. Iannotti, G. Edwin Wilson, Laurence V. Madden, Harry A.J. Hoitink
Department of Plant Pathology: Faculty Publications
We report the use of a model system that examines the dynamics of biological energy availability in organic matter in a sphagnum peat potting mix critical to sustenance of microorganism-mediated biological control of pythium root rot, a soilborne plant disease caused by Pythium ultimum. The concentration of readily degradable carbohydrate in the peat, mostly present as cellulose, was characterized by cross-polarized magic-angle spinning 13C nuclear magnetic resonance spectroscopy. A decrease in the carbohydrate concentration in the mix was observed during the initial 10 weeks after potting as the rate of hydrolysis of fluorescein diacetate declined below a critical threshold level …
Promoters From Chlorellavirus Genes Providing Expression Of Genes In Prokaryotic And Eukaryotic Hosts, Amitava Mitra, James L. Van Etten
Promoters From Chlorellavirus Genes Providing Expression Of Genes In Prokaryotic And Eukaryotic Hosts, Amitava Mitra, James L. Van Etten
Department of Plant Pathology: Faculty Publications
The invention is directed to novel promoters or mutants thereof from Chlorella virus DNA methyltransferase genes. A Chlorella virus gene promoter is operably linked to a first and/or second DNA sequence encoding a gene that is different from the Chlorella virus gene to form an expression cassette. An expression cassette can be introduced into prokaryotic and/or eukaryotic cells and can provide for a high level of expression of the gene encoded by the first and/or second DNA sequence. The invention also provides a method for screening other Chlorella virus genes for promoters that can function to express a heterologous gene …
Taking Root, W. John Hayden
Taking Root, W. John Hayden
Biology Faculty Publications
The brief description of mangrove reproduction in John N. Cole's "Off Key, Out of Reach" [A Sense of Place, July-August] contains an error.
The Method Of Radicle Emergence In Phytolacca Americana (Pokeweed), Staci A. Van Winkle
The Method Of Radicle Emergence In Phytolacca Americana (Pokeweed), Staci A. Van Winkle
Honors Theses
Germination is a key element in the development of a plant. This process encompasses several stages, one of which is the elongation of the radicle tip. This process has been noted to occur by different methods such as rapid mitotic division or cell elongation. However, such a mechcmism has not been studied in Phytolacca americana (pokeweed). It was the intention of this study to observe at the cellular level the nature of the Phytolacca americana radicle during normal germination. This was done using a routine histological microtechnique which involves embedding in paraffin and sectioning with a microtome. The sections were …
Biotin Carboxyl Carrier Protein And Carboxyltransferase Subunits Of The Multi-Subunit Form Of Acetyl-Coa Carboxylase From Brassica Napus: Cloning And Analysis Of Expression During Oilseed Rape Embryogenesis, Kieran M. Elborough, Robert Winz, Ranjit K. Deka, Jennifer E. Markham, Andrew J. White, Stephen Rawsthorne, Antoni R. Slabas
Biotin Carboxyl Carrier Protein And Carboxyltransferase Subunits Of The Multi-Subunit Form Of Acetyl-Coa Carboxylase From Brassica Napus: Cloning And Analysis Of Expression During Oilseed Rape Embryogenesis, Kieran M. Elborough, Robert Winz, Ranjit K. Deka, Jennifer E. Markham, Andrew J. White, Stephen Rawsthorne, Antoni R. Slabas
Department of Biochemistry: Faculty Publications
In the oilseed rape Brassica napus there are two forms of acetyl- CoA carboxylase (ACCase). As in other dicotyledonous plants there is a type I ACCase, the single polypeptide 220 kDa form, and a type II multi-subunit complex analogous to that of Escherichia coli and Anabaena. This paper describes the cloning and characterization of a plant biotin carboxyl carrier protein (BCCP) from the type II ACCase complex that shows 61% identity/79% similarity with Anabaena BCCP at the amino acid level. Six classes of nuclear encoded oilseed rape BCCP cDNA were cloned, two of which contained the entire coding region. …
Morphometric Analysis And Comparisons Of Electrophoretic Protein Profiles Of The Scutellaria Ovata Hill (Lamiaceae) Complex In West Virginia, Virginia And Ohio With Emphasis On Shale Barren Taxa, Eric W. Ewing
Theses, Dissertations and Capstones
Elements of the Scutellaria ovata complex in the eastern United States are reported to include S. ovata var. pseudoarguta, S. ovata var. rugosa, S. ovata var. virginiana and S. ovata ssp. ovata. All but S. ovata ssp. ovata occur in the shale barren habitat of eastern West Virginia and western Virginia. S. ovata var. pseudoarguta has been proposed as a threatened element in North America. A morphometric study of specimens collected in West Virginia, Virginia and Ohio was undertaken to determine which characters are most useful in separating taxa in the complex. Nineteen characters were assessed from 104 herbarium specimens …
Floristics, Plant Community Structure And Bank Profiles, For Five Sites Along The Middle Ohio River, Amy Nicole Morris
Floristics, Plant Community Structure And Bank Profiles, For Five Sites Along The Middle Ohio River, Amy Nicole Morris
Theses, Dissertations and Capstones
Plant community structure, floristics, and bank profiles for five sites along the middle Ohio River were described. Values obtained were compared with a similar study by Clagg and Mills in 1978 on four of the five sites. A total of 186 plant species was observed in the plant community and floristic study. Glenwood Bend had the greatest diversity with 109 plant species. Mason-Pomeroy had the lowest diversity with only 35 plant species. Six species, representing 3.2 percent of the total observed number of species, were present at all five sites. The majority of species, 51 percent, were present at only …
Evapotranspiration From A Saltcedar-Dominated Desert Floodplain: A Scaling Approach, S. D. Smith, A. M. Sala, Dale A. Devitt
Evapotranspiration From A Saltcedar-Dominated Desert Floodplain: A Scaling Approach, S. D. Smith, A. M. Sala, Dale A. Devitt
Life Sciences Faculty Research
The purpose of this study was to investigate evapotranspiration (ET) from a variety of scales (leaf to landscape) in saltcedar-dominated floodplain vegetation along the lower Virgin River of southern Nevada. Leaf-level gas exchange indicated that saltcedar exhibits similar stomatal conductance as the sympatric phreatophytes arrowweed, mesquite, and willow. However, sap flow in saltcedar was higher per unit sapwood area than the other species, suggesting that it maintains higher leaf area per unit sapwood area. At the stand level, saltcedar ET was found to exceed potential ET early in the summer when soils were moist and the water table was near …
Weeds Can Poison Crops, Aik Hock Cheam
Weeds Can Poison Crops, Aik Hock Cheam
Journal of the Department of Agriculture, Western Australia, Series 4
Weeds can interfere with the growth of a crop simply by competing with the crop for nutrients, moisture and light. But some weeds also release chemicals that inhibit the germination and growth of crop plants; the technical term for this is allelopathy. Aik Cheam outlines the problems caused by two common weeds and the discusses preventative measures.
Germplasm And Cultivar Development, M. D. Casler, Jeffrey F. Pedersen, G. C. Eizenga, S. D. Stratton
Germplasm And Cultivar Development, M. D. Casler, Jeffrey F. Pedersen, G. C. Eizenga, S. D. Stratton
Department of Agronomy and Horticulture: Faculty Publications
Cool-season forage grasses have evolved, and continue to evolve, in natural ecosystems subject to environmental factors both in the presence and absence of human influences. The literature often lacks facts describing the evolution and domestication of forage grasses. Furthermore, the literature on this subject mainly deals with evolution of species in the broad scope, i.e., on a scale of hundreds of thousands or millions or years. Thus, some of our conclusions are necessarily speculative and are highly subject to the nature of the research that has been reported. We describe the forces of selection that act upon cool-season forage grasses …
The Complete Nucleotide Sequence And Genome Organization Of The M Rna Segment Of Peanut Bud Necrosis Tospovirus And Comparison With Other Tospoviruses, Satyanarayana Tatineni, S. E. Mitchell, D. V. R. Reddy, S. Kresovich, R. Jarret, R. A. Naidu, S. Gowda, J. W. Demski
The Complete Nucleotide Sequence And Genome Organization Of The M Rna Segment Of Peanut Bud Necrosis Tospovirus And Comparison With Other Tospoviruses, Satyanarayana Tatineni, S. E. Mitchell, D. V. R. Reddy, S. Kresovich, R. Jarret, R. A. Naidu, S. Gowda, J. W. Demski
Department of Plant Pathology: Faculty Publications
The M RNA of peanut bud necrosis virus (PBNV; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. It comprised two ORFs in an ambisense organization and terminal inverted repeats. The 3' large ORF (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kDa which was identified as the glycoprotein precursor (GP) of the G1 and G2 glycoproteins. A comparison of the deduced amino acid sequence of GP revealed 37 % identity and 58-59% similarity with that of tomato spotted wilt virus (TSWV, serogroup I) and impatiens necrotic spot virus (INSV, serogroup III), …
Peanut Bud Necrosis Virus: Purification Of Nucleocapsids And Sequence Homology Of Nucleocapsid Protein And Glycoprotein Precursor With Other Tospoviruses, Satyanarayana Tatineni, S. E. Mitchell, D. V. R. Reddy, S. Brown, S. Kresovich, R. Jarret, S. Gowda, R. A. Naidu, J. W. Demski
Peanut Bud Necrosis Virus: Purification Of Nucleocapsids And Sequence Homology Of Nucleocapsid Protein And Glycoprotein Precursor With Other Tospoviruses, Satyanarayana Tatineni, S. E. Mitchell, D. V. R. Reddy, S. Brown, S. Kresovich, R. Jarret, S. Gowda, R. A. Naidu, J. W. Demski
Department of Plant Pathology: Faculty Publications
A procedure for the purification of peanut bud necrosis virus (PBNV) nucleocapsids was developed. Virus particles were treated with nonionic detergent to disrupt the envelope membrane and free nucleocapsids were separated. into three lightscattering zones after sucrose gradient centrifogation. Nucleocapsids from the top zone contained S RNA and traces ofM RNA; whereas, nucleoplasids from the middle zone contained M RNA with detectable levels of S RNA and those from the bottom zone contained L RNA with traces of M and S RNAs. Clones from a cDNA library made from the purified PBNV Sand M RNAs were characterized and sequenced. Comparison …