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The Tomato U-Box Type E3 Ligase Pub13 Acts With Group Iii Ubiquitin E2 Enzymes To Modulate Fls2-Mediated Immune Signaling, Bangjun Zhou, Lirong Zeng

Department of Plant Pathology: Faculty Publications

In Arabidopsis and rice, the ubiquitin ligase PUB13-mediated protein degradation plays a significant role in plant pattern-triggered immunity (PTI) and flowering time control. The Arabidopsis PUB13 has been shown to attenuate the pattern recognition receptor FLS2-mediated immune signaling by ubiquitinating FLS2 and consequently promoting its degradation by the 26S proteasome. Nevertheless, the cognate ubiquitin-conjugating enzymes (E2) with which PUB13 acts to modulate FLS2-mediated PTI are unknown. To address this question, we investigate here the tomato (Solanum lycopersicum) homolog of PUB13, SlPUB13 by utilizing the recently characterized complete set of tomato E2s. Of the 13 groups of tomato E2s, …

Proteomic Analysis Of Ubiquitinated Proteins In Rice (Oryza Sativa) After Treatment With Pathogen-Associated Molecular Pattern (Pamp) Elicitors, Xiao-Lin Chen, Xin Xie, Liye Wu, Caiyun Liu, Lirong Zeng, Xueping Zhou, Feng Luo, Guo-Liang Wang, Wende Liu

Department of Plant Pathology: Faculty Publications

Reversible protein ubiquitination plays essential roles in regulating cellular processes. Although many reports have described the functions of ubiquitination in plant defense responses, few have focused on global changes in the ubiquitome. To better understand the regulatory roles of ubiquitination in rice pattern-triggered immunity (PTI), we investigated the ubiquitome of rice seedlings after treatment with two pathogen-associated molecular patterns, the fungal-derived chitin or the bacterialderived flg22, using label-free quantitative proteomics. In chitin-treated samples, 144 and 167 lysine-ubiquitination sites in 121 and 162 proteins showed increased and decreased ubiquitination, respectively. In flg22-treated samples, 151 and 179 lysine-ubiquitination sites in 118 and …

Investigating Phosphorylation Patterns And Their Effect On The Activity Of Transcription Factor Taabf1 In Imbibing Cereal Grains, Grace Uwase

Honors Theses

The wheat transcription factor TaABF1 plays an important role in hormone-mediated regulation of seed dormancy and germination of cereal grains. Evidence shows that TaABF1 activity is regulated by phosphorylation, and previous work in our lab showed that when serine residues in its conserved regions; S36, S37, S113, S115 were altered to phosphomimetic aspartate, the 4xD TaABF1 mutant had increased activity as a transcription factor. However, when only S113 was altered, TaABF1’s activity was greatly reduced. The work presented here explored whether the S36D/S37D/S115D mutant would have stronger activity than the 4xD mutant. Using the particle bombardment technique to introduce the …

Expression Analyses And Identification Of Key Molecular Participants In Plant Responses To Environmental Cues, Praveena Kanchupati

Electronic Theses and Dissertations

Plants constantly engage and interact with the environment and respond to the changes in conditions like temperature, water, and photoperiod, by regulating expression of genes of multiple regulatory and signaling pathways. Insight into these pathways and their participants has provided and will provide candidates to improve various agronomically important traits in crops through marker-assisted breeding and genetic manipulation. With this aim in mind, in the present study, I attempted to identify key candidate genes that are involved in the regulation of; i) plant response to low temperature stress, ii) plant roots’ response to soil moisture content and iii) flowering time. …

Terrestrial Coarse Woody Debris, Erin C. Rowekamp, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Lonicera Maackii Berry Leachate Toxicity: Physicochemical Data, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

In-stream (in situ) and in-lab microcosm trials using Hyalella azteca, Anthopotamus verticis, and Allocapnia spp.

Characterization Of Palmitoyltransferase Proteins In Arabidopsis Thaliana, Danielle Mcginty

Honors Theses and Capstones

No abstract provided.

Application Of Machine Learning Methodologies For Predicting Corn Economic Optimal Nitrogen Rate, Zhisheng Qin, D. Brenton Myers, Curtis J. Ransom, Newell R. Kitchen, Sang-Zi Liang, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A. M. Laboski, Brad D. Malone, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Determination of in-season N requirement for corn (Zea mays L.) is challenging due to interactions of genotype, environment, and management. Machine learning (ML), with its predictive power to tackle complex systems, may solve this barrier in the development of locally based N recommendations. The objective of this study was to explore application of ML methodologies to predict economic optimum nitrogen rate (EONR) for corn using data from 47 experiments across the US Corn Belt. Two features, a water table adjusted available water capacity (AWC_wt) and a ratio of in-season rainfall to AWC_wt (RAWC_wt), were …

Seed Versus Bulb, Indoor Or Outdoor, Joy Lee

Natural Sciences Student Research Presentations

This poster for the Natural Sciences Poster Session at Parkland College summarizes the student's experiment growing seed plants (arugula) and bulb plants (crocus), both indoors and outdoors, using height of the seed as a measurement.

Mac3a And Mac3b, Two Core Subunits Of The Mos4-Associated Complex, Positively Influence Mirna Biogenesis, Shengjun Li, Kan Liu, Bangjun Zhou, Mu Li, Shuxin Zhang, Lirong Zeng, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MAC3A and MAC3B are conserved U-box containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis. However, their functional mechanisms remain elusive. Here we show that Arabidopsis thaliana MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs ( …

Regulation Of Local Auxin Metabolism During Soybean Nodule Development, Suresh Damodaran

Electronic Theses and Dissertations

Legume-rhizobia symbiosis leads to the development of secondary root organs called nodules. Rhizobia bacteria housed inside nodules assimilate atmospheric nitrogen and convert them into plant usable forms thereby reducing the need for fertilizer application in crop legumes like soybean. Nodule development is a coordinated process orchestrated by multiple plant hormones. In soybean, the auxin responsive gene expression was detected in nodule primordia and in the periphery of mature nodules, primarily in nodule vasculature. Auxin hypersensitivity reduces nodule formation in soybean and also polar auxin transport inhibition at the site of nodule development is not crucial for determinate nodule formation. Therefore, …

Identification Of Qtl Modifying The Activity Of The Tcb1-S Locus And Characterization And Sequencing Of Two Plutonium-Beryllium Induced Reduced Gametophyte Transmission Mutants In Maize, Merritt Bryer Burch

Electronic Theses and Dissertations

This thesis is split into two independent projects both involving the male gametophyte generation of maize. The first project looks at how pollen interacts with the female gametophyte to reduce its transmission in cross-incompatible reactions controlled by the unilateral cross-incompatibility system, teosinte crossing barrier 1. The second project explores two plutonium-beryllium induced male gametophyte mutants and attempts to uncover their genetic basis.

Identification of QTL Modifying the Activity of the Tcb1-s Locus
Teosinte crossing barrier 1 (Tcb1) is a unilateral cross-incompatibility system present in maize that provides a pre-zygotic pistil barrier to plants carrying Tcb1-s (strong allele) from pollen …

Water Toxicity Effects Test: Survival And Growth (Dry Mass), Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Sediment Toxicity Effects Test, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Wetted Width, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Within-Stream Variability Data, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Water Toxicity Effects Test: Physicochemical Data, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Sediment % Solids And Total Organic Carbon, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Site Characteristics, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

Stream order, HHEI, Watershed size, Forest Cover, Maximum pool depth

Lonicera Maackii Leaf Leachate Toxicity: Survival, Eric B. Borth, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

Lymnaea stagnalis and Hyalella azteca

Sapling, Erin C. Rowekamp, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Lonicera Maackii Berry Leachate Toxicity: Survival And Mass, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

In-stream (in situ) and in-lab microcosm trials using Hyalella azteca, Anthopotamus verticis, and Allocapnia spp.

Honeysuckle, Erin C. Rowekamp, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Probe Data, Charlotte A. Shade, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

Temperature, Dissolved oxygen, Conductivity, pH, Total dissolved solids

Terrestrial Fine Woody Debris, Erin C. Rowekamp, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Herbaceous Vegetation: August, Julia I. Chapman, Margaret E. Maloney, Erin C. Rowekamp, Mitchell J. Kukla, Sean D. Mahoney, Eric B. Borth, Ryan W. Mcewan

Data Files

August

Time To Colonization Trial Data, Margaret E. Maloney, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Lonicera Maackii Flower Leachate Toxicity: Physicochemical Data, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

In-stream (in situ) and in-lab microcosm trials using Hyalella Azteca and Anthopotamus verticis

Twig And Fruit Litter Basket, Erin C. Rowekamp, Taylor M. Buskey, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Lonicera Maackii Berry Leachate Toxicity: Length, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

In-stream (in situ) and in-lab microcosm trials using Hyalella azteca, Anthopotamus verticis, and Allocapnia spp.