Digital Commons Network^™

Short Term Effects Of A Pectoralis Minor Positional Release In Collegiate Swimmers: A Case Series, Jonathan Roman, Noelle M. Selkow

Clinical Practice in Athletic Training

A tight pectoralis minor correlates to abnormal scapular kinematics, which can cause pain, loss of range of motion and even loss of function, due to the change in scapular position. With these muscular imbalances causing forward scapular posture, the pectoralis minor is a key component to address in the prevention and treatment of shoulder impingement and scapular dyskinesis. This study investigated the effectiveness of a single positional release therapy (PRT) treatment of the pectoralis minor on scapular posture in collegiate swimmers immediately and 24 hours post intervention. Seventeen Division III collegiate swimmers (7 males and 10 females) volunteered to participate, …

Expanding Clinical Practice With Complementary And Integrative Health, Such As Tai Chi, As Therapeutic Interventions, Connor A. Burton

Clinical Practice in Athletic Training

No abstract provided.

Lrrk2 Deficiency Impairs Trans-Golgi To Lysosome Trafficking And Endocytic Cargo Degradation In Human Renal Proximal Tubule Epithelial Cells, Nathan J. Lanning, Calvin Vanopstall, Megan L. Goodall, Jeffrey P. Mackeigan

University Faculty Publications and Creative Works

Defects in vesicular trafficking underlie a wide variety of human diseases. Genetic disruption of leucine-rich repeat kinase 2 (LRRK2) in rodents results in epithelial vesicular trafficking errors that can also be induced by treatment of animals with LRRK2 kinase inhibitors. Here we demonstrate that defects in human renal cells lacking LRRK2 phenocopy those seen in the kidneys of Lrrk2 knockout mice, characterized by accumulation of intracellular waste vesicles and fragmentation of the Golgi apparatus. This phenotype can be recapitulated by knockdown of N-ethylmaleimide-sensitive factor, which physically associates with LRRK2 in renal cells. Deficiency in either protein leads to a defect …

The Acceptance And Action Questionnaire – Ii: An Item Response Theory Analysis, Clarissa W. Ong, Benjamin G. Pierce, Douglas W. Woods, Michael P. Twohig, Michael E. Levin

Psychology Faculty Publications

Psychological flexibility is the act of being open to internal experiences while pursuing valued life directions and has been implicated in positive mental health. A lack of psychological flexibility has been implicated in a wide range of mental health problems. In most research, assessment of psychological (in)flexibility has been done with the Acceptance and Action Questionnaire – II (AAQ-II), yet researchers have noted that items on the AAQ-II may not adequately discriminate between responses to experiences and the experiences themselves. Furthermore, little research has examined whether items on the AAQ-II function as intended in terms of assessing psychological (in)flexibility, and …

Space Operations In The Suborbital Space Flight Simulator And Mission Control Center: Lessons Learned With Xcor Lynx, Pedro Llanos, Christopher Nguyen, David Williams, Kim O. Chambers, Erik Seedhouse, Robert Davidson

Journal of Aviation/Aerospace Education & Research

This study was conducted to better understand the performance of the XCOR Lynx vehicle. Because the Lynx development was halted, the best knowledge of vehicle dynamics can only be found through simulator flights. X-Plane 10 was chosen for its robust applications and accurate portrayal of dynamics on a vehicle in flight. The Suborbital Space Flight Simulator (SSFS) and Mission Control Center (MCC) were brought to the Applied Aviation Sciences department in fall 2015 at Embry-Riddle Aeronautical University, Daytona Beach campus. This academic and research tool is a department asset capable of providing multiple fields of data about suborbital simulated flights. …

Single Men's Experiences Of Coping With Financial Stress, Heath Downes

Walden Dissertations and Doctoral Studies

Experiencing financial hardship is stressful and can lead to the development of psychological symptoms, including depression and anxiety. Although researchers have examined the broad categories of coping strategies that people employ when faced with financial hardship, little is known about the experiences of single men who were able to navigate stressful times without developing anxiety or anxiety-like symptoms. The purpose of this phenomenological study was to explore the successful coping experiences of single men with financial hardship (e.g., loss of job, home, finances). Bonanno's theory of resilience provided the framework for the study. Data were collected from interviews with 12 …

Environmental And Physiological Regulation Of Yellow Stingray Color Change, Theresa Rose Gunn

College of Graduate Studies: Theses & Dissertations

Many reef fishes exhibit dynamic coloration and body patterns that can change under nervous or hormonal control. Several species of benthic sharks and rays likely alter melanin in the skin to provide background matching for camouflage. The yellow stingray (Urobatis jamaicensis) is a benthically-oriented elasmobranch with elaborate spot patterns that provide effective camouflage within its habitats. This patterning, when coupled with the ability to alter melanin in response to background color, could increase background matching effectiveness in these species. The yellow stingray has been anecdotally noted to lighten or darken skin color.However, it is unclear whether this type …

Structure Of The Picornavirus Replication Platform: A Potential Drug Target For Inhibiting Virus Replication, Meghan Suzanne Warden

Chemistry & Biochemistry Theses & Dissertations

Picornaviruses are small, positive-stranded RNA viruses, divided into twelve different genera. Members of the Picornaviridae family cause a wide range of human and animal diseases including the common cold, poliomyelitis, foot and mouth disease, and dilated cardiomyopathy. The picornavirus genome is replicated via a highly conserved mechanism involving a presumed cloverleaf structure located at the 5’ noncoding region of the virus genome. The 5’ cloverleaf consists of three stem loops (B, C, and D) and one stem (A), which interact with a variety of virus and host cell proteins during replication. In this dissertation, human rhinovirus serotype 14 (HRV-14) SLB …

Molecular Candidates For Blocking The Transmission Of Vector-Borne Diseases, Ashish Naresh Vora

Biological Sciences Theses & Dissertations

Vector-borne diseases (VBDs) require urgent attention as they have a high mortality rate, with 700,000 people dying in a year as of 2017. So far, extremely few reliable vaccination strategies and solutions have emerged. Transmission blocking vaccines are one among many solutions available to manage the control of VBDs. These require identification of both vector and pathogen molecules that play important roles in the transmission, dissemination and establishment of VBDs. Our strategy is to characterize these candidates that play important roles in vector-host-pathogen interactions. This manuscript presents two such studies identifying the participation of two arthropod molecules in vector-host-pathogen interactions. …

Effects Of Knee Sleeves On Knee Mechanics During Squats At Variable Depths, Alexandria A. Trypuc

Human Movement Studies & Special Education Theses & Dissertations

The squat is a functional, compound and multi-joint exercise that targets several muscles of the lower body and is widely used in both athletics and many exercise programs. This exercise has been the subject of many studies, comparing different squat variations and examining how external gear, such as squat suits and knee wraps impact the exercise. The aim of this study was to assess the effects of wearing neoprene knee sleeves on lower extremity kinematics, kinetics, and muscle activations during weighted back squats. Fifteen resistance trained men and women, aged 28±5 years, from the local fitness community and university campus …

Lack Of Delayed-Type Hypersensitivity In Striped Bass (Morone Saxatilis), Jessica Shannon Miller

Biological Sciences Theses & Dissertations

Mycobacteriosis is a fatal disease in fishes caused by acid-fast bacteria in the genus Mycobacterium. This disease impacts aquaculture, aquariums, and wild fishes. Unfortunately, there are currently no non-lethal diagnostic tests for mycobacterial infection in fishes. Type IV delayed type hypersensitivity (DTH) responses in vivo and in vitro are currently used for the non-lethal detection of mycobacterial infections in humans and in animals; however, there is little information available on DTH responses in fishes. In this work, we examine in vivo DTH response in Striped Bass (Morone saxatilis), an important U.S. Eastern seaboard fish species, experimentally …

Molecular Characterization Of The Mummichog (Fundulus Heteroclitus) Ovarian Steroidogenic Pathway And Implications For Exogenous Estrogen Effects During Follicular Development, Thiviya Kanagasabesan

Theses and Dissertations (Comprehensive)

This study investigated ovarian development in Fundulus heteroclitus (mummichog), through molecular characterization of key mediators in the ovarian steroidogenic pathway; these included ovarian StAR (steroidogenic acute regulatory protein), cytochrome P450 enzymes, estrogen receptors (ERα, ERβ1 and ERβ2), and gonadotropin receptors (FSHr and LHr); additionally, hepatic vitellogenin (VTG1) and ERs, and brain P450 aromatase (P450arom; CYP19b) were studied. Fish were staged into cortical alveolus, vitellogenic, early mature, late mature, and ovulating stages of maturation; blood (plasma), ovary (tissue and isolated ovarian follicles per stage), liver, and brains were collected. Testosterone (T), 17β-estradiol (E₂) and maturation inducing steroid (MIS), measured …

The Fatigue-Associated Changes In Estimates Of Persistent Inward Current In Human Motor Neurons, Chris Compton

Theses and Dissertations (Comprehensive)

Neuromuscular fatigue is associated with reduced supraspinal drive. Similarly, intracortical facilitation and muscle activation are reduced following concussion. Both fatigue and mild traumatic brain injury are associated with increased noradrenergic and serotonergic activity in animal models. Given that monoaminergic-dependent persistent inward currents (PIC) set spinal motor neuron (MN) gain, we speculate that PIC will increase during fatigue to compensate for supraspinal hypoexcitability and that this will be more pronounced in people with concussion. Therefore, the purpose of this experiment was to assess spinal MN excitability during fatigue in people with concussion and healthy controls. 20 participants (10 concussion, average age …

Gill Structure & Function In Parasitic And Non-Parasitic Lampreys: The Effects Of Metamorphosis And Freshwater-Seawater Transfer, Julia Sunga

Theses and Dissertations (Comprehensive)

Lamprey (Petromyzontiformes) are a phylogenetically ancient group of jawless fishes that begin their lives as filter-feeding larvae (ammocoetes) before undergoing a complex metamorphosis into juvenile lamprey that involves major changes to their internal and external body plan. Some parasitic species, such as the sea lamprey (Petromyzon marinus), migrate to sea following metamorphosis, where they use their oral discs and rasping tongue to attach to and ingest vast quantities of blood from fishes. Thus, sea lamprey have to counter the simultaneous challenges of hyposmoregulation in sea water and the generation of large quantities of ammonia due to the …

Rpe Vs. Percentage 1rm Loading In Periodized Programs Matched For Sets And Repetitions, Eric R. Helms, Ryan K. Byrnes, Daniel M. Cooke, Michael H. Haischer, Joseph P. Carzoli, Trevor K. Johnson, Matt R. Cross, John B. Cronin, Adam G. Storey, Michael C. Zourdos

Research outputs 2014 to 2021

Purpose: To investigate differences between rating of perceived exertion (RPE) and percentage one-repetition maximum (1RM) load assignment in resistance-trained males (19-35 years) performing protocols with matched sets and repetitions differentiated by load-assignment. Methods: Participants performed squats then bench press 3x/weeks in a daily undulating format over 8-weeks. Participants were counterbalanced by pre-test 1RM then assigned to percentage 1RM (1RMG, n = 11); load-assignment via percentage 1RMs, or RPE groups (RPEG, n = 10); participant-selected loads to reach target RPE ranges. Ultrasonography determined pre and post-test pectoralis (PMT), and vastus lateralis muscle thickness at 50 (VLMT50) and 70% (VLMT70) femur-length. Results: …

Strengthening The Practice Of Exercise And Sport-Science Research, Israel Halperin, Andrew D. Vigotsky, Carl Foster, David B. Pyne

Research outputs 2014 to 2021

Exercise and sport sciences continue to grow as a collective set of disciplines investigating a broad array of basic and applied research questions. Despite the progress, there is room for improvement. A number of problems pertaining to reliability and validity of research practices hinder advancement and the potential impact of the field. These problems include inadequate validation of surrogate outcomes, too few longitudinal and replication studies, limited reporting of null or trivial results, and insufficient scientific transparency. The purpose of this review is to discuss these problems as they pertain to exercise and sport sciences based on their treatment in …

Influence Of Maturation Status On Eccentric Exercise-Induced Muscle Damage And The Repeated Bout Effect In Females, Ming-Ju Lin, Kazunori Nosaka, Chih-Chiao Ho, Hsin-Lian Chen, Kuo-Wei Tseng, Sebastien Ratel, Trevor Chung-Ching Chen

Research outputs 2014 to 2021

This study compared changes in indirect muscle damage markers, proprioception and arterial stiffness after elbow flexor eccentric exercise between pre-pubescent (9–10 y), pubescent (14–15 y), and post-pubescent (20–24 y) healthy, untrained females (n = 13/group). The maturation of the participants was confirmed by the hand bone age. All participants performed two bouts of 30 sub-maximal eccentric contractions (EC1, EC2) using a dumbbell set at 60% of pre-exercise maximal voluntary isometric elbow flexion strength at 90°. Changes in maximal voluntary concentric contraction (MVC) torque, muscle soreness (SOR), plasma creatine kinase activity, proprioception (position sense, joint reaction angle) and arterial stiffness …

Serine-Dependent Sphingolipid Synthesis Is A Metabolic Liability Of Aneuploid Cells, Sunyoung Hwang, H. Tobias Gustafsson, Ciara O’Sullivan, Gianna Bisceglia, Xinhe Huang, Christian Klose, Andrej Schevchenko, Robert C. Dickson, Paola Cavaliere, Noah Dephoure, Eduardo M. Torres

Molecular and Cellular Biochemistry Faculty Publications

Aneuploidy disrupts cellular homeostasis. However, the molecular mechanisms underlying the physiological responses and adaptation to aneuploidy are not well understood. Deciphering these mechanisms is important because aneuploidy is associated with diseases, including intellectual disability and cancer. Although tumors and mammalian aneuploid cells, including several cancer cell lines, show altered levels of sphingolipids, the role of sphingolipids in aneuploidy remains unknown. Here, we show that ceramides and long-chain bases, sphingolipid molecules that slow proliferation and promote survival, are increased by aneuploidy. Sphingolipid levels are tightly linked to serine synthesis, and inhibiting either serine or sphingolipid synthesis can specifically impair the fitness …

Acute Resistance Exercise Induces Sestrin2 Phosphorylation And P62 Dephosphorylation In Human Skeletal Muscle, Nina Zeng, Randall F. D'Souza, Vandre C. Figueiredo, James F. Markworth, Llion A. Roberts, Jonathan M. Peake, Cameron J. Mitchell, David Cameron-Smith

Center for Muscle Biology Faculty Publications

Sestrins (1, 2, 3) are a family of stress-inducible proteins capable of attenuating oxidative stress, regulating metabolism, and stimulating autophagy. Sequestosome1 (p62) is also a stress-inducible multifunctional protein acting as a signaling hub for oxidative stress and selective autophagy. It is unclear whether Sestrin and p62^Ser403 are regulated acutely or chronically by resistance exercise (RE) or training (RT) in human skeletal muscle. Therefore, the acute and chronic effects of RE on Sestrin and p62 in human skeletal muscle were examined through two studies. In Study 1, nine active men (22.1 ± 2.2 years) performed a bout of single-leg strength …

Macrophage Migration Inhibitory Factor Mediates Protease-Activated Receptor 4-Induced Bladder Pain Through Urothelial High Mobility Group Box 1, Fei Ma, Dimitrios E. Kouzoukas, Katherine L. Meyer-Siegler, David E. Hunt, Lin Leng, Richard Bucala, Pedro L. Vera

Physiology Faculty Publications

Macrophage migration inhibitory factor (MIF) mediates pain although the mechanisms are not well understood. Urothelial activation of protease activated receptor 4 (PAR4) results in urothelial MIF release, urothelial high mobility group box 1 (HMGB1) release and bladder pain in mice without bladder inflammation. All three effects are prevented by MIF inhibition while intravesical disulfide HMGB1 alone can induce bladder pain. This study utilizes genetic MIF deletion to determine whether MIF mediates PAR4-induced bladder pain and is upstream of HMGB1-induced bladder pain. Wild type (C57/BL6) and MIF knockout (KO) mice were treated with intravesical PAR4 activating peptide or disulfide HMGB1 and …

Organizational Effects Of Defeminizing Toxicants: Lessons Learned From An Environmental Sentinel Organism, The Fathead Minnow., Jonathan Ali

Theses & Dissertations

Endocrine disrupting compounds (EDCs) are chemicals that interfere with hormone function and are increasingly detected in aquatic environments, where they elicit adverse effects from exposed organisms. The toxicological effects of EDCs can be described as either activational (reversible) or organizational (irreversible), where the latter are associated with adverse outcomes in reproductive performance of adult fish. However, few studies have investigated the organizational impacts of anti-estrogenic or “defeminizing” EDCs, e.g. agrichemicals or pharmacological agents, in an environmentally-relevant or “sentinel” species. The objective of this study was to investigate the impacts of early-life EDC-initiated changes in estrogenic gene expression on organizational effects …

Response Of Appetite And Appetite Regulating Hormones To Acute Hypoxia, Morgan Busboom, Robert Shute, Dustin Slivka

Journal of Human Performance in Extreme Environments

AIM: To determine the acute response of appetite and appetite regulating hormones after exposure to simulated altitude. METHODS: Seven males and five females (height: 178.9 ± 2.3 cm; weight: 77.3 ± 7.2 kg; body fat: 18.4 ± 1.7%) participated in two, three-hour trials in a hypoxic (5000 m) and normoxic (350 m) environment. Blood samples were collected prior to and immediately following three hours of exposure for the measurement of leptin, adiponectin, and acylated ghrelin. Appetite, acute mountain sickness, heart rate, blood oxygenation, tissue oxygenation, respiration rate, and whole body gases were also measured. RESULTS: Leptin was not different between …

The Vibe, Sarah P. Douglass

Capstones

The Vibe is a long-form narrative about where tech is taking the female orgasm. The piece concludes that physiological research is a required next step when creating the climax of the future.

http://sarahpdouglass.com

Cholesterol Regulation Of Pulmonary Endothelial Calcium Entry Following Chronic Hypoxia, Bojun Zhang

Biomedical Sciences ETDs

Chronic hypoxia (CH)-induced pulmonary hypertension (PH) is associated with diminished ATP-induced endothelial Ca2+ entry as well as membrane cholesterol in pulmonary arteries. Store-operated Ca2+ entry (SOCE) and depolarization-induced Ca2+ entry are major components of the response to ATP and are similarly decreased after CH. Because endothelium-dependent vasodilation is closely associated with pulmonary endothelial [Ca2+]i, the blunted agonist-induced Ca2+ influx in pulmonary artery endothelial cells (PAEC) may contribute to the development of CH-induced PH. Interestingly, impaired agonist-induced Ca2+ influx in PAEC following CH can be restored by membrane cholesterol supplementation. In the current studies, we hypothesized that impaired Ca2+ entry in …

Methylglyoxal Requires Ac1 And Trpa1 To Produce Pain And Spinal Neuron Activation, Ryan B. Griggs, Don E. Laird, Renee R. Donahue, Weisi Fu, Bradley K. Taylor

Physiology Faculty Publications

Methylglyoxal (MG) is a metabolite of glucose that may contribute to peripheral neuropathy and pain in diabetic patients. MG increases intracellular calcium in sensory neurons and produces behavioral nociception via the cation channel transient receptor potential ankyrin 1 (TRPA1). However, rigorous characterization of an animal model of methylglyoxal-evoked pain is needed, including testing whether methylglyoxal promotes negative pain affect. Furthermore, it remains unknown whether methylglyoxal is sufficient to activate neurons in the spinal cord dorsal horn, whether this requires TRPA1, and if the calcium-sensitive adenylyl cyclase 1 isoform (AC1) contributes to MG-evoked pain. We administered intraplantar methylglyoxal and then evaluated …

Hyperhomocysteinemia-Induced Gene Expression Changes In The Cell Types Of The Brain, Erica M. Weekman, Abigail E. Woolums, Tiffany L. Sudduth, Donna M. Wilcock

Sanders-Brown Center on Aging Faculty Publications

High plasma levels of homocysteine, termed hyperhomocysteinemia, are a risk factor for vascular cognitive impairment and dementia, which is the second leading cause of dementia. While hyperhomocysteinemia induces microhemorrhages and cognitive decline in mice, the specific effect of hyperhomocysteinemia on each cell type remains unknown. We took separate cultures of astrocytes, microglia, endothelial cells, and neuronal cells and treated each with moderate levels of homocysteine for 24, 48, 72, and 96 hr. We then determined the gene expression changes for cell-specific markers and neuroinflammatory markers including the matrix metalloproteinase 9 system. Astrocytes had decreased levels of several astrocytic end feet …

Translating Genetic And Preclinical Findings Into Autism Therapies, Maria Chahrour, Robin Kleiman, M Chiara Manzini

Pharmacology and Physiology Faculty Publications

Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder characterized by social deficits and repetitive/restrictive interests. ASD is associated with multiple comorbidities, including intellectual disability, anxiety, and epilepsy. Evidence that ASD is highly heritable has spurred major efforts to unravel its genetics, revealing possible contributions from hundreds of genes through rare and common variation and through copy-number changes. In this perspective, we provide an overview of the current state of ASD genetics and of how genetic research has spurred the development of in vivo and in vitro models using animals and patient cells to evaluate the impact of genetic mutations …

Resting Hormone Alterations And Injuries: Block Vs “Daily Undulating Periodization” Weight-Training Among Division I Track And Field Athletes, Keith B. Painter, Gregory N. Haff, Travis Triplett, Charles A. Stuart, Guy Hornsby, Michael W. Ramsey, Caleb D. Bazyler, Michael H. Stone

ETSU Faculty Works

No abstract provided.

Exercise, Cognition, And The Aging Process Among Active, Competitive, And Sedentary Middle-Aged And Older Adults, Katherine Adams

Health, Human Performance and Recreation Undergraduate Honors Theses

Purpose This study aimed to compare the cognitive measures of reaction time and dual-task performance among recreationally active, master athletes, and sedentary older adults aged 50 years or older. Methods 59 late middle-aged and older adults between the ages of 50 to 88 years old participated in reaction time and dual-task tests of which consisted of multiple trials on the same day. Subjects were placed either into the recreationally active, masters athlete, or sedentary activity level group based on the Rapid Assessment of Physical Activity (RAPA) form. For the gait speed with associated dual-task component, subjects walked 10-meters at two …

Worked To Leisure: The Intersections Of Volunteerism, Work, And Leisure, Denise Nicole Cook

UNLV Theses, Dissertations, Professional Papers, and Capstones

Volunteerism and work are topics which academics in several disciplines research. This study is an examination of the intersection of work and volunteerism via the lens of role theory. According to role theorists, the term role refers to behaviors which are either a result of social position or expectations (Biddle 1986). Cognitive role theorists use vocabulary such as “role conflict” and “role overload” to identify incongruences sometimes found in roles individuals have in their lives (Biddle 1986). These incongruences are primarily examined as they relate to individual level consequences. This research explores volunteerism and work as they relate to role …