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Evaluation Of Load Velocity Profiles With Varying Warm-Up Sets And Relative Intensities, Gabriel L. Perez, Samantha V. Narvaez, Joshua Carrillo, Misael Duque, Noel F. Medrano, Brett S. Nickerson

International Journal of Exercise Science

International Journal of Exercise Science 14(4): 971-979, 2021. The purpose of this study was to determine whether the number of warm-up sets and relative intensity impacts the prediction of velocity-based one-repetition maximum (1RM) values. Twenty resistance-trained individuals (males: n = 10, females: n = 10) participated in this study. Warm-up sets consisted of subject’s bench-pressing loads at 50 (five-repetitions), 70 (three-repetitions), and 90% (one-repetition) of estimated 1RM. A maximum of four attempts were performed to determine 1RM, while recording mean concentric velocity (MCV)using a linear position transducer during warm-up and 1RM trials in order to develop load-velocity profiles. Specifically, four …

Transcranial Direct Current Stimulation With The Halo Sport Does Not Improve Performance On A Three-Minute High Intensity Cycling Test, Collin Garner, Rachel Dykstra, Nicholas J. Hanson, Michael G. Miller

International Journal of Exercise Science

International Journal of Exercise Science 14(3): 962-970, 2021. Transcranial direct current stimulation (tDCS) uses a weak electrical current that is sent through the cerebral cortex. The Halo Sport headphones are a user-friendly form of tDCS that is implemented by many athletes purportedly to improve performance. The purpose of this study was to determine the effect of tDCS, using the Halo Sport, on performance variables associated with a high-intensity three-minute cycling test. Eighteen healthy, active individuals (ten men, eight women) volunteered for this study. The Halo Sport headphones were worn during a 20-minute warmup before completing a high-intensity three-minute cycling test. …

Notch Signaling Represses Cone Photoreceptor Formation Through The Regulation Of Retinal Progenitor Cell States, Xueqing Chen, Mark M. Emerson

Publications and Research

Notch signaling is required to repress the formation of vertebrate cone photoreceptors and to maintain the proliferative potential of multipotent retinal progenitor cells. However, the mechanism by which Notch signaling controls these processes is unknown. Recently, restricted retinal progenitor cells with limited proliferation capacity and that preferentially generate cone photoreceptors have been identified. Thus, there are several potential steps during cone genesis that Notch signaling could act. Here we use cell type specific cis-regulatory elements to localize the primary role of Notch signaling in cone genesis to the formation of restricted retinal progenitor cells from multipotent retinal progenitor cells. Localized …

Late Embryogenesis Abundant Proteins And Mitochondrial Membranes From An Animal Extremophile: Insights Into Severe Water Stress, John Marcus Anderson

LSU Doctoral Dissertations

This dissertation focuses on two major topics germane to mechanisms by which animals tolerate extreme water stress. First, the impact of transgenic expression of late embryogenesis abundant proteins (LEA) on water stress tolerance of the fruit fly Drosophila melanogaster was investigated. The overall aim was to extend current understanding of the protective properties of LEA proteins documented with isolated cells to a desiccation-sensitive organism during exposure to drying and hyperosmotic stress. Four lines of D. melanogaster were created that expressed transgenes encoding selected LEA proteins originally identified in embryos of the extremophile Artemia franciscana. After 80% tissue water loss, …

Temperature-Induced Activation Of The Reproductive Axis Through Melatonin-Mediated Changes In Thyrotropin, Treven J. Winters

Dissertations and Theses

An animal's ability to synchronize life-history events or stages with optimal environmental conditions is paramount to successfully reproducing and maximizing fitness. Additional events including migration, foraging, rearing of young, and emergence from hibernation are all examples of processes under environmental control in one species or another. An exciting new mechanism involving neural thyroid hormone metabolism has been elucidated that links environmental control to the neuroendocrine reproductive axis. In birds and mammals with seasonal breeding cycles, this neuroendocrine pathway is activated by photoperiod-induced changes in thyroid stimulating hormone (TSH, also known as thyrotropin) production within the pars tuberalis region of the …

Black And White Health Disparities: Racial Bias In American Healthcare, Yasmeen Almomani

Bridges: An Undergraduate Journal of Contemporary Connections

This paper explores the historical implications of race in American society that have led to implicit racism in the healthcare system. Racial bias in healthcare against Black people is a factor in the health disparities between Black and white people in America, such as the gap in life expectancy, infant death, and maternal mortality. Black people are more likely to report racial discrimination from healthcare providers, which is a reason for the decreased quality of care received. The past justifications of slavery, the Tuskegee syphilis study, and the medical experimentations on Black women are horrifying but were considered acceptable in …

Prediction And Cross-Validation Of An Energy Expenditure Equation In Walking Or Running In Asian Adults, Xi Jin, Teresa Carithers, Mark Loftin

International Journal of Exercise Science

International Journal of Exercise Science 14(7): 932-940, 2021. The prevalence of obesity is increasing across the world. Knowledge of the actual energy expenditure (EE) of walking and running can lead to a more precise exercise prescription which may contribute to obesity reduction or avoidance. Limited research has focused on EE prediction during walking or running in Asian adults. So, the aims of this study included developing an EE prediction equation and cross-validating the equation for Asian adults. Methods: A total of 85 Asians participated to test EE through indirect calorimetry. Linear regression analysis was employed for EE prediction, and a …

Developing A Comprehensive Testing Battery For Mixed Martial Arts, Matthew G. Plush, Stuart N. Guppy, Kazunori Nosaka, Oliver R. Barley

International Journal of Exercise Science

International Journal of Exercise Science 14(4): 941-961, 2021. Mixed martial arts (MMA) is a combat sport that employs techniques from different combat disciplines. There are a multitude of technical and physiological characteristics that contribute to competitive success. Developing a single scientific assessment that can predict competitive outcomes poses great difficulty due to the complexity of MMA. While previous research has investigated some important physiological characteristics, there is no accepted best-practice for a comprehensive testing battery. As such, this study aimed to design and utilize a battery of physiological assessments to cover aerobic and anaerobic function, strength measures including explosive and …

Anthropometric And Leg Power Factors Affect Offensive Kinetic Patterns In Fencing, Anastasia Ntai, Charilaos Tsolakis, Athanasia Smirniotou, Giorgos Paradisis

International Journal of Exercise Science

International Journal of Exercise Science 14(4): 919-931, 2021. This study aimed to identify potential factors that may influence specific fencing offensive kinetic patterns in a large group of well-trained fencers having different ages, gender level, and training specialization. One-hundred-thirty fencers (males: n = 72) and (females: n = 58), participating in three different fencing weapons (epee, foil, and sabre), having considerable experience of national and international competitions. All members of seven national fencing teams were measured for basic anthropometric parameters, leg power performance and velocity values for three specific kinetic offensive patterns during an International Fencing camp. A multivariate analysis …

The Effect Of Freeze-Thaw Events On Dna Integrity In The Gray Treefrog (Hyla Versicolor), Georgia Ficarra

Masters Theses & Specialist Projects

Freeze-tolerant gray treefrogs survive winter by producing natural cryoprotectants and accommodating ice formation within extracellular spaces. While frozen, gray treefrogs endure hyperglycemia, dehydration, and anoxia due to the halt of all bodily functions. Upon thawing, the frogs’ anoxic cells receive a rapid influx of oxygen, which can cause oxidative damage to vital macromolecules including DNA. Previous studies have suggested freeze-tolerant frogs avoid oxidative damage after freeze-thaw events by elevating antioxidant activity, but recent work has shown upregulated DNA repair encoding genes in post-freeze frogs. The objective of this thesis is to assess the cellular costs of freezing by measuring oxidative …

Blood Plasma Biomarkers For Woody Breast Disease In Commercial Broilers, Byungwhi C. Kong, Bhuwan Khatri, Seong Kang, Stephanie Shouse, Hakeem J. Kadhim, Michael Thomas Kidd Jr., Kentu Lassiter, Joseph Hiltz, Barbara Mallmann, Sara Orlowski, Nicholas Anthony, Walter G. Bottje, Wayne Kuenzel, Casey Owens

Poultry Science Faculty Publications and Presentations

Woody breast (WB) myopathy results in poor muscle quality. The increasing incidence of WB over the last several years indicates a need for improved prediction or early diagnosis. We hypothesized that the use of body fluids, including blood, may be more suitable than breast muscle tissue in developing a minimally invasive diagnostic tool for WB detection. To identify potential early-age-biomarkers that may represent the potential onset of WB, blood samples were collected from 100, 4 wks old commercial male broilers. At 8 wks of age, WB conditions were scored by manual palpation. A total of 32 blood plasma samples (eight …

Computational Analysis Of Type 3 Iodothyronine Deiodinase: Potential Inhibitors, Substrate Binding, And Dimer Structure, Eric Scott Marsan

Chemistry & Biochemistry Theses & Dissertations

Thyroid hormones (THs) in mammalian tissues are crucial for development and maintaining metabolic homeostasis. Iodothyronine deiodinases (Dios) remove iodines from THs by a selenocysteine (Sec) residue, which either activates or inactivates them. Halogen bonding (XB) has been proposed to describe the interaction between the Se and I atoms of the T4-Dio complex. Disruption of TH homeostasis by xenobiotics, such as polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) can cause deleterious effects on the endocrine system. Experimental studies have indicated that PBDEs and PCBs could disrupt TH homeostasis by inhibiting Dio through XB formation. However, no current quantitative study exists …

Effect Of A 2-Week Relaxing Music Intervention On Anxiety, Stress, And Gut Symptoms In Aerobic Exercisers, Halie Marisa Maass

Human Movement Studies & Special Education Theses & Dissertations

The purpose of this study was to determine the impact of a two-week relaxing music intervention on stress, anxiety, and gastrointestinal (GI) symptoms in exercisers with at least mild anxiety and exercise-related GI symptoms. Block randomization was used to allocate 15 women and two men into control (n = 8) or intervention (n = 9) groups following a one-week baseline. During the baseline period, participants recorded details of their aerobic exercise sessions and discomfort with GI symptoms (nausea, regurgitation/reflux, stomach fullness, bloating, abdominal cramps, gas, the urge to defecate). Following randomization, both groups tracked these same details for two additional …

Heat Stress And Feed Restriction Distinctly Affect Performance, Carcass And Meat Yield, Intestinal Integrity, And Inflammatory (Chemo)Cytokines In Broiler Chickens, Nima K. Emami, Elizabeth S. Greene, Michael H. Kogut, Sami Dridi

Poultry Science Faculty Publications and Presentations

This study was conducted to distinguish the effects of heat stress (HS) and feed intake (FI) on broiler chicken’s physiological responses. Day-old male Cobb 500 broilers (n = 672) were allocated to three treatments: (1) control (CTL): birds raised under normal temperature (23°C) from day 29 to 42; (2) cyclic heat stress (CHS): birds exposed to high temperatures (8 h/day at 35°C; from 9:30 am to 5:30 pm) from day 29 to 42; (3) pair-fed (PF): birds raised under thermoneutral condition but fed the same amount of feed as CHS from day 29 to 42. On day 42, 15 …

Role Of Nhe3 In The Intestine And Thick Ascending Limb Of The Kidneys, Jianxiang Xue

USF Tampa Graduate Theses and Dissertations

The sodium-hydrogen exchanger isoform 3 (NHE3, SLC9A3) mediates most Na+/H+ exchange in the gastrointestinal tract and the kidney, where it plays crucial roles in Na+ and fluid absorption as well as acid-base homeostasis. Whole body NHE3 knockout (NHE3-/-) mice show overt absorptive defects in both the intestine and kidneys. Genetic mutations of NHE3 gene in humans are associated with congenital sodium diarrhea (CSD). In the kidneys, NHE3 is expressed in the proximal tubule (PT) and the thick ascending limb (TAL). Whole kidney-specific (PT and TAL) NHE3 knockout (NHE3Pax8cre) mice and PT-specific NHE3 knockout (PT-NHE3-/-) mice have shown the importance of …

Reduced Mitochondrial Dna And Oxphos Protein Content In Skeletal Muscle Of Children With Cerebral Palsy, Ferdinand Von Walden, Ivan J. Vechetti Jr., Davis A. Englund, Vandré C. Figueiredo, Rodrigo Fernandez-Gonzalo, Kevin A. Murach, Jessica Pingel, John J. Mccarthy, Per Stål, Eva Pontén

Physiology Faculty Publications

AIM: To provide a detailed gene and protein expression analysis related to mitochondrial biogenesis and assess mitochondrial content in skeletal muscle of children with cerebral palsy (CP).

METHOD: Biceps brachii muscle samples were collected from 19 children with CP (mean [SD] age 15y 4mo [2y 6mo], range 9-18y, 16 males, three females) and 10 typically developing comparison children (mean [SD] age 15y [4y], range 7-21y, eight males, two females). Gene expression (quantitative reverse transcription polymerase chain reaction [PCR]), mitochondrial DNA (mtDNA) to genomic DNA ratio (quantitative PCR), and protein abundance (western blotting) were analyzed. Microarray data sets (CP/aging/bed rest) were …

The Neurological Asymmetry Of Self-Face Recognition, Aleksandra Janowska, Brianna Balugas, Matthew Pardillo, Victoria Mistretta, Katherine Chavarria, Janet Brenya, Taylor Shelansky, Vanessa Martinez, Kitty Pagano, Nathira Ahmad, Samantha Zorns, Abigail Straus, Sarah Sierra, Julian Keenan

Department of Biology Faculty Scholarship and Creative Works

While the desire to uncover the neural correlates of consciousness has taken numerous directions, self-face recognition has been a constant in attempts to isolate aspects of self-awareness. The neuroimaging revolution of the 1990s brought about systematic attempts to isolate the underlying neural basis of self-face recognition. These studies, including some of the first fMRI (functional magnetic resonance imaging) examinations, revealed a right-hemisphere bias for self-face recognition in a diverse set of regions including the insula, the dorsal frontal lobe, the temporal parietal junction, and the medial temporal cortex. In this systematic review, we provide confirmation of these data (which are …

Metabolic Rate, Critical Oxygen Partial Pressure, And Oxygen Supply Capacity Of Farfantepenaeus Duorarum At Their Lower Thermal Limit, Alexandra L. Burns

USF Tampa Graduate Theses and Dissertations

Temperature and environmental oxygen availability affect oxygen supply and demand in ectotherms, which are hypothesized to control the geographic limits of many marine species. The oxygen supply capacity (α) is calculated from commonly measured metabolic traits, including the standard metabolic rate (SMR) and critical oxygen partial pressure at SMR (Pcrit). It may be used to estimate the metabolic capacity and aerobic scope across changes in temperature and oxygen partial pressures as α reflects adaptations of the cardiorespiratory system to meet maximum energy demands at a given oxygen partial pressure (PO2). In this study, α was measured for the Tampa Bay …

A Study Of Trpa1 Activation Via Covalent Electrophilic Modification Of Cysteines, Thomas A. Parks

USF Tampa Graduate Theses and Dissertations

A subset of sensory nerves called nociceptors are stimulated by noxious stimuli and evoke nocifensive responses in different organ systems. Transient Receptor Potential Ankyrin 1 (TRPA1) is a tetrameric, nonselective, cation channel that initiates the generator potential that evokes afferent signaling in nociceptive nerves. TRPA1 is activated many harmful irritants, such as food chemicals, environmental pollutants, reactive oxygen species and other endogenous mediators. Most TRPA1 agonists have electrophilic properties, which covalently modify cysteine residues on the cytosolic side of the channel. Biochemical studies in our lab have identified four cysteines (C273, C621, C665, C1085) that rapidly bound iodoacetamide (irreversible electrophile); …

Cardiomyocyte Deletion Of Bmal1 Exacerbates Qt- And Rr-Interval Prolongation In Scn5a+/Δkpq Mice, Elizabeth A. Schroder, Jennifer L. Wayland, Kaitlyn M. Samuels, Syed F. Shah, Don E. Burgess, Tanya S. Seward, Claude S. Elayi, Karyn A. Esser, Brian P. Delisle

Physiology Faculty Publications

Circadian rhythms are generated by cell autonomous circadian clocks that perform a ubiquitous cellular time-keeping function and cell type-specific functions important for normal physiology. Studies show inducing the deletion of the core circadian clock transcription factor Bmal1 in adult mouse cardiomyocytes disrupts cardiac circadian clock function, cardiac ion channel expression, slows heart rate, and prolongs the QT-interval at slow heart rates. This study determined how inducing the deletion of Bmal1 in adult cardiomyocytes impacted the in vivo electrophysiological phenotype of a knock-in mouse model for the arrhythmogenic long QT syndrome (Scn5a+/ΔKPQ). Electrocardiographic telemetry showed inducing the …

The Effect Of Fluid Flow Shear Stress And Substrate Stiffness On Yes-Associated Protein (Yap) Activity And Osteogenesis In Murine Osteosarcoma Cells, Thomas R. Coughlin, Ali Sana, Kevin Voss, Abhilash Gadi, Upal Basu-Roy, Caroline M. Curtin, Alka Mansukhani, Oran D. Kennedy

Publications and Research

Osteosarcoma (OS) is an aggressive bone cancer originating in the mesenchymal lineage. Prognosis for metastatic disease is poor, with a mortality rate of approximately 40%; OS is an aggressive disease for which new treatments are needed. All bone cells are sensitive to their mechanical/ physical surroundings and changes in these surroundings can affect their behavior. However, it is not well understood how OS cells specifically respond to fluid movement, or substrate stiffness—two stimuli of relevance in the tumor microenvironment. We used cells from spontaneous OS tumors in a mouse engineered to have a bone-specific knockout of pRb-1 and p53 in …

Demographics, Activities, And Environmental Factors Impact Burnout In A National Survey Of Emergency Medicine Residents, Nicole Battaglioli, Tim P. Moran, Simiao Li-Sauerwine

Journal of Wellness

Introduction: Burnout in emergency medicine and in residency training has been well-described. The impact of demographic, individual, and programmatic factors on burnout have not previously been determined in a national survey of emergency medicine residents. This study aimed to identify personal and environmental factors impacting resident burnout in a national sample of emergency medicine residents.

Methods: A prospective Emergency Medicine Resident Wellness Survey was administered in 2017. We surveyed respondents on demographic, personal, and environmental factors; each respondent also completed the Maslach Burnout Inventory - Human Services Survey. Linear regressions were used to identify variables associated with the Maslach Burnout …

Ice Hockey Goaltender Physiology Profile And Physical Testing: A Systematic Review And Meta-Analysis, Viviane Marcotte-L'Heureux, Jérémie Charron, Robert Panenic, Alain Steve Comtois

International Journal of Exercise Science

International Journal of Exercise Science 14(6): 855-875, 2021. This review aims to 1) be the first systematic review and meta-analysis of the literature examining the physiology and assessment of goaltenders, and 2) present a physiological profile of ice-hockey goaltenders. It will 1) highlight physiological differences between goaltenders and players at other positions, 2) determine strengths and weaknesses of ice hockey goaltenders, and 3) offer possible guidelines for strength and conditioning coaches. Six electronic databases were systematically searched in October 2019 using the PRISMA model. A total of twelve scientific articles published in peer-reviewed journals were included. Professional male (PM) goaltenders …

The Effect Of Set Configuration And Load On Post-Activation Potentiation On Vertical Jump In Athletes, Paolo Sirieiro, Igor Nasser, Ward C. Dobbs, Jeff Willardson, Humberto Miranda

International Journal of Exercise Science

International Journal of Exercise Science 14(4): 902-911, 2021. The aim of this study was to compare the effect of post-activation potentiation (PAP) on countermovement jump (CMJ) using different set configurations and loads on conditioning activity (CA) in highly trained athletes. Sixteen national level swimmers participated in this study and performed a total of six visits to the laboratory. The first session was used for familiarization, the second session was utilized to determine five repetitions maximum (RM) in the half squat (HS), and the following four visits consisted of four CA protocols performed in a counterbalanced order. Two CAs were performed …

Are There Differences In Fitness Between Recruits From Larger (Hosting) And Smaller (Participating) Law Enforcement Agencies?, Erika Hernandez, J. Jay Dawes, Robin Marc Orr, Joseph Dulla, Robert G. Lockie

International Journal of Exercise Science

International Journal of Exercise Science 14(4): 885-901, 2021. Law enforcement agencies often conduct academy training classes that include recruits hired by both the larger hosting agency (HA), and by smaller participating agencies (PA). HAs that need to fill more positions may have recruits with a wide range of fitness levels. Smaller agencies may be more selective in their hiring as they do not have as many available positions. This study compared HA and PA recruit fitness prior to academy. Retrospective analysis was conducted on 11 academy classes, incorporating 742 HA recruits (602 males, 140 females) and 99 PA recruits (82 …

A Community-Based Boxing Program Is Associated With Improved Balance In Individuals With Parkinson’S Disease, Abbie Moore, Enoch Yee, Brad W. Willis, Evan L. Prost, Aaron D. Gray, J. Bryan Mann

International Journal of Exercise Science

International Journal of Exercise Science 14(3): 876-884, 2021. In alignment with efforts to mitigate the negative health consequences of Parkinson’s Disease (PD), the purpose of this investigation was to examine if participation in a community-based boxing program (CBP) was associated with improvements in balance and fall risk reduction among individuals with PD. In this retrospective cross-sectional study, de-identified data from 12 individuals with PD participating in a CBP was examined. Participants included those with a Hoehn and Yahr stage between 1 and 3, averaging 2.8 ± 0.8 CBP sessions per week for 6.1 ± 0.8 months between testing. Baseline and …

Hip Flexion Angles During Supine Range Of Motion And Bodyweight Squats, Angeli J. Mata, Hidetaka Hayashi, Phillip A. Moreno, Robert I. Dudley, Eric A. Sorenson

International Journal of Exercise Science

International Journal of Exercise Science 14(1): 912-918, 2021. During the lowering phase of a squat, it has been observed that a posterior pelvic tilt (PPT) may occur when squatting to full depth. Research suggests that defaulting to compensatory movement strategies, such as PPT, during the squat may correlate with risk of lower extremity and trunk pathology. The purpose of this study was to examine hip flexion (HF) angles at the point when PPT occurs among three conditions: standard squats, heel raise squats, and supine passive HF; analyzing the differences in depth between standard and heel raise squats; and calculating differences …

The Effects Of Fasting Or Ketogenic Diet On Endurance Exercise Performance And Metabolism In Female Mice, Lola Holcomb, Caitlin O'Neill, Elizabeth Dewitt, Stephen C. Kolwicz Jr.

Health Sciences Faculty Publications

The promotion of ketone body (KB) metabolism via ketosis has been suggested as a strategy to increase exercise performance. However, studies in humans and animals have yielded inconsistent results. The purpose of the current study was to examine the effects of ketosis, achieved via fasting or a short-term ketogenic diet (KD), on endurance exercise performance in female mice. After 8 h of fasting, serum KB significantly increased and serum glucose significantly decreased in fasted compared to fed mice. When subjected to an endurance exercise capacity (EEC) test on a motorized treadmill, both fed and fasted mice showed similar EEC performance. …

Racial And Ethnic Minority Groups Are Under-Represented And Under-Reported In Guideline-Informing Heart Failure Clinical Trials, Gerardo Gamino

Undergraduate Honors Theses

Heart failure (HF) is a common cause of morbidity and mortality in the United States that may disproportionately affect certain racial/ethnic groups. Compared with White individuals, HF may affect Black individuals at a younger age with less favorable prognosis, and this excess risk may be partially explained by differences in HF risk factor burden. It is crucial for guideline informing HF clinical trials to adequately reflect the racial/ethnic diversity in the population. We assessed the extent of reporting and representation of race/ethnicity in HF clinical trials referenced in the 2013 ACC/AHA Guideline for the Management of Heart Failure and the …

Training On A Lower Body Positive Pressure Treadmill With Body Weight Support Does Not Improve Aerobic Capacity, David K. Lee, Natalie Maltz, James Becker, Joshua A. Cotter

International Journal of Exercise Science

International Journal of Exercise Science 14(7): 829-839, 2021. This study examined the physiological changes resulting from training on a lower body positive pressure treadmill (LBPPT) at three different levels of body weight support (BWS). Thirty-three healthy college aged students (22.3 ± 3.1 years) completed the study. Participants performed a graded exercise test (GXT) to exhaustion and were placed into one of three experimental groups corresponding to 100%, 75%, and 50% of their normal BW. Participants trained at their experimental BW levels for eight-weeks. Training speed was monitored by heart rate (HR) and speed was adjusted to elicit approximately 60% of …