Digital Commons Network^™

The Structure Of A Complex Of Bovine &-Thrombin And Recombinant Hirudin At 2.8-A Resolution, Jacqueline Vitali, Philip D. Martin, Michael G. Malkowski, William D. Robertson, Jerome B. Lazar, Richard C. Winant, Paul H. Johnson, Brian F.P. Edwards

Physics Faculty Publications

Crystals of the complex of bovine alpha-thrombin with recombinant hirudin variant 1 have space group C222(1) with cell constants a = 59.11, b = 102.62, and c = 143.26 A. The orientation and position of the thrombin component was determined by molecular replacement and the hirudin molecule was fit in 2 magnitude of Fo - magnitude of Fc electron density maps. The structure was refined by restrained least squares and simulated annealing to R = 0.161 at 2.8-A resolution. The binding of hirudin to thrombin is generally similar to that observed in the crystals of human thrombin-hirudin. Several differences in …

The Gulf Stream Front, Its Role In Larval Fish Survival And Recruitment In Florida: Hydrographic Station And Plankton Data, Denis William Frazel, M. Elizabeth Clarke, G. S. Kleppel, Peter B. Ortner, John M. Braker, Carol A. Burkart, Gayle Louise Stone

Technical Reports

The goal of this project was to develop a clearer understanding of the role that the Gulf Stream system plays in larval fish survival and recruitment in Florida waters. The specific objectives of this study were to:

1. Determine whether the biomass of fish larva, other zooplankton, microzooplankton and phytoplankton is higher at the shoreward front of the Gulf Stream than on either side of it.
2. Characterize the assemblages of fish larvae predators and prey both at the front and on either side of it.
3. Determine if the composition, abundance and size frequency distribution of larval and juvenile fishes in the …

Parallel Cellular Automata: A Model Program For Computational Science, Per Brinch Hansen

Electrical Engineering and Computer Science - Technical Reports

We develop a model program for parallel execution of cellular automata on a multicomputer. The model program is then adapted for simulation of forest fires and numerical solution of Laplace's equation for stationary heat flow. The performance of the parallel program is analyzed and measured on a Computing Surface configured as a matrix of transputers with distributed memory.

Wright State University College Of Engineering And Computer Science Bits And Pcs Newsletter, Volume 8, Number 6, September 1992, College Of Engineering And Computer Science, Wright State University

BITs and PCs Newsletter

An eight page newsletter created by the Wright State University College of Engineering and Computer Science that addresses the current affairs of the college.

The Immunosuppressive And Toxic Effects Of Fk-506 Are Mechanistically Related: Pharmacology Of A Novel Antagonist Of Fk-506 And Rapamycin, Francis J. Dumont, Mary Jo Staruch, Samuel L. Koprak, John Siekierka, C. Shirley Lin, Richard Harrison, Tonya Sewell, Victoria M. Kindt, Thomas R. Beattie, Matthew Wyvratt, Nolan H. Sigal

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

FK-506 inhibits Ca2+-dependent transcription of lymphokine genes in T cells, and thereby acts as a powerful immunosuppressant. However, its potential therapeutic applications may be seriously limited by several side effects, including nephrotoxicity and neurotoxicity. At present, it is unclear whether these immunosuppressive and toxic effects result from interference with rehted biochemical processes. FK-506 is known to interact with FK-binding protein-12 (FKBP-12), an abundant cytosolic protein with cis.trans peptidyl-prolyl isomerase activity (PPlase) activity. Because rapamycin (RAP) similarly binds to FKBP-12, although it acts in a manner different from FK-506, by inhibiting T cell responses to lymphokines, such an interaction with FKBP-12 …

The Probe, Issue 125 - September 1992

The Probe: Newsletter of the National Animal Damage Control Association

THE PROBE
National Animal Damage Control Association
SEPTEMBER 1992
Bird Strike Committee USA Is Born
Calendar of Upcoming Events
Positions Available
"Wire" Grid Excludes Cormorants from Commercial Catfish Ponds
"Pesticides to 1995" Available
Animal Damage Control in the News:
Arizona Update From WLFA
Ducks Scared For Own Good
California Activists Convicted For Tule Elk Hunt Disruption
Ace Hardware Stops Support for “Kind News” Publication
Attack on California Girl, 5, Results in Coyote Hunt
Norplant for Beavers?
Fifth Eastern Wildlife Damage Control Conference Proceedings.

The Prairie Naturalist Volume 24, No.3 September 1992

The Prairie Naturalist

VESICULAR-ARBUSCULAR MYCORRHIZAE IN BLOWOUT PENSTEMON ▪ T. R. Flessner and J. Stubbendieck

STATUS OF HAPLOPAPPUS FREMONTII A. GRAY SSP. MONOCEPHALUS (A. NELSON) HALL [ASTERACEAE] IN COLORADO ▪ K.A. Schulz and R.B. Shaw

HABITAT CHANGES ABOVE AND BELOW WATER PROJECTS ON THE NORTH PLATTE AND SOUTH PLATTE RIVERS IN NEBRASKA ▪ P. M. McDonald and J.G. Sidle

INSECT FLORAL VISITORS TO FOUR SPECIES OF TALL-GRASS PRAIRIE COMPOSITE (ASTERACEAE: HELIANTHEAE) ▪ J. A. Dickinson and M. J. McKone

SUMMER BIRD USE OF KANSAS WINDBREAKS ▪ T.T. Cable, R.L. Schroeder, V. Brack, Jr., and P.S. Cook

RING-NECKED PHEASANTS AND FOOD PLOT SIZE (GALLIFORMES: …

Field Calibration Of A Transient Model For Broiler Misting, Richard S. Gates, Douglas G. Overhults, Robert W. Bottcher, Shu Hui Zhang

Biosystems and Agricultural Engineering Faculty Publications

A transient model to predict temperature within a tunnel ventilated broiler house during misting is developed. The model is calibrated with field data to obtain steady-state constants; transient predictions are compared to measured temperatures during cyclic misting for two different size birds. Measured temperatures during cyclic misting are shown to swing between steady-state asymptotes predicted from the model. Transient response of the model was faster than measured temperature data, in part due to temperature sensor dynamic response. The model predicts the length-wise temperature profile within the building during misting, and can be used to investigate alternate misting strategies and designs. …

Design Of An Artificial Neural Network Based Tactile Sensor For The Utah/Mit Dexterous Hand, Jeffery D. Nering

Theses and Dissertations

The Neural Tactile Sensor (NTS) is a high resolution, easily manufactured tactile sensor consisting of electrodes, a thin resistive 'skin', and pattern recognition circuitry that is capable of resolving dynamic and static contact location, force, and slip throughout the continuum of the sensor's active region. The sensor operates by means of a resistive 'skin' harboring the electric field generated when a current is injected into it, and a plurality of electrodes for taking measurements of said electric field. When current flows through the resistive medium from the location of tactile contact, an electric field within the resistive medium is established, …

Risk Estimation And Expert Judgment: The Case Of Yucca Mountain, Kristin Shrader-Frechette

RISK: Health, Safety & Environment (1990-2002)

Professor Shrader-Frechette discusses factors responsible for acute disagreement between the federal government and Nevada citizens over potential Risks at Yucca Mountain and focuses on the use of expert judgment, concluding that some of them appear to exemplify "bad science." That aside, she argues that 1,000 year predictions cannot be made from current knowledge of geology or, e.g., institutional behavior and concludes that permanent disposal of radioactive waste is currently impossible.

Autonomous Face Segmentation, Kevin P. Gay

Theses and Dissertations

The purpose of this study was to implement an autonomous face segmentor as the front end to a face recognition system on a Sun SPARCStation2. Face recognition performance criteria, specifically, the capabilities to isolate and resize faces in an image to a consistent scale, were analyzed to determine current practical limitations. Face images were acquired using a S-VHS camcorder. Segmentation was accomplished using motion detection and predefined rules. Tests were run to determine the suitable of the autonomous segmentor as the front-end to a face recognition system. The segmentation system developed consistently located faces and rescaled those faces to a …

Interim Nutrient Budgets For Tampa Bay, Gerold Morrison

Reports

This report addresses one task of the nutrient monitoring project, the development of "interim" (i.e., preliminary) annual budgets for the macronutrients nitrogen and phosphorus in Tampa Bay's major sub-basins. For the purposes of the report, those sub-basins are defined as Hillsborough Bay (HB) , Old Tampa Bay (OTB) , Middle Tampa Bay (MTB) , and Lower Tampa Bay (LTB). The report uses the 1 Environmental Protection commission of Hillsborough County (EPCHC) database and other available info:mation in,an attempt to identify and quantify major sources and slnks for nltrogen and phosphorus within the Tampa Bay system.

The report is organized in …

The Euclidean Distance Transform (Thesis), Ching-Shoei Chiang

Department of Computer Science Technical Reports

No abstract provided.

Compact Suffix Trees Resemble Patricia Tries: Limiting Distribution Of Depth, Philippe Jacquet, Bonita Rais, Wojciech Szpankowski

Department of Computer Science Technical Reports

No abstract provided.

34th Rocky Mountain Conference On Applied Spectroscopy

Rocky Mountain Conference on Magnetic Resonance

Program and registration information for the 34th annual meeting of the Rocky Mountain Conference on Applied Spectroscopy, co-sponsored by the Colorado Section of the American Chemical Society and the Rocky Mountain Section of the Society for Applied Spectroscopy. Held in Denver, Colorado, August 2-6, 1992.

John Muir Newsletter, Fall 1992, John Muir Center For Regional Studies

Muir Center Newsletters (1981-2015)

John Muir Newsletter university of the pacific fall, 1992 NATURE AND THE HUMAN SPIM volume 2, number 4 '» TOTIfi TRANSFORMATION OF HENRY LOOMIS by Ron Limbaugh A few years ago, while working on a research project at Yale's Sterling Memorial Library, I ran across a 90-page handwritten journal written by Henry Bradford Loomis, son of a prominent Yale mathematician and astronomer, an irresolute companion of John Muir during a three-month trip to Alaska in 1890. In labored but legible longhand, Loomis chronicled the expedition.' It is a fascinating record. Not only does it provide a starkly contrasting view to …

The Probe, Issue 124 - August 1992

The Probe: Newsletter of the National Animal Damage Control Association

THE PROBE
National Animal Damage Control Association
AUGUST 1992
The Public, Coyotes, and Wildlife Damage Management
Calendar of Upcoming Events
Tenth Great Plains Wildlife Damage Control Workshop Proceedings.
Animal Damage Control in the News:
Pickups Seized in Federal Raid
Colorado Phases Out Spring Bear Hunt
WLFA Works to Defeat Proposed Arizona Hunting/Trapping Ban
DWRC Attempting to Develop Deer Baits
Canadian Trappers' Delegation Attends ISO Meeting
Professional trappers College
Public, Coyotes, & Wildlife Damage Management
News from the Northeast
The Coyote by Baxter Black

Biomonitoring Study Of A Constructed Wetland Site Treating Acid Mine Drainage, Barbara A. Ramey, Howard G. Halverson, Linda A. Taylor

KWRRI Research Reports

Acid mine drainage (AMD) from an underground coal mine in the Jones Branch watershed in McCreary County, KY, substantially reduced water quality in Jones Branch. Downstream from the mine seeps, the pH was routinely below 4.5 and concentrations of most heavy metals, especially iron, were elevated. A cattail wetland (1,022 m²) was constructed on Jones Branch in 1989 to obviate the effects of the AMD. Monthly chemical monitoring was performed on the water from above, from below, and from the 26 cells within the wetland. Based on chemical monitoring, the wetland initially improved water quality, increasing the pH …

Water Current, Volume 24, August 1992

Water Current Newsletter

Future Water Quality Protection, Coordination, Education, Research and Regulatory Activities Needed
NRDs Commemorate 50 Millionth Tree
Report from the Director: Water Research Top Priority
National Water Information Clearinghouse Workshops Issue Executive Report
What Do We Do Till the Well Runs Dry?
Missouri River Basin Tour Focuses on Surface Water Management in South Dakota and North Dakota
1992 Nebraska Water Legislation
Hardy-Superior Spa Water Quality Regulations
Study: Most Farmers Protect Groundwater
Nebraska Water Groups and Associations

Dispiro- 1,2,4,5-Tetraoxanes: A New Class Of Antimalarial Peroxides, Jonathan L. Vennerstrom, Hong-Ning Fu, Walter Reed Army Institute Of Research, Arba L. Ager Jr., James K. Wood

Chemistry Faculty Publications

Dispiro-l,2,4,5tetraoxanes2 -4 were synthesized as potential peroxide a n t h a l a* d drugs. They had curative activity against Plasmodium berghei in vivo at single doses of 320 and 640 mg/kg which confirms earlier unpublished data Moreover, artemisinin (1) and 4 had equivalent ED”s against P. berghei in vivo in the multiple-dose Thompson tat; neither showed any evidence of acute toxicity at total doses of more than 12 g/kg. Dispiro-l,2,4,5-tetraoxane 4 had IC₅₀’s comparable to those of 1 against Plasmodium falciparum clones in vitro. These results confirm the potential of dispiro-l,2,4,5-tetraoxanes as a new class of …

Behavior Of Nutrients And Trace Metals In Muck Sediments From A Florida Lagoon, Huan Feng

Theses and Dissertations

Fine-grained, organic-rich sediments are a potentially importment reservoir for nutrients and contaminants in the Indian River Lagoon, Florida. However, little is known about the behavior of these chemical species and whether they can be released to the overlying water column. In that regard, this study had two objectives: (1 ) to determine the concentrations of dissolved nitrogen and phosphorus species and some metals in representative muck deposits and the fluxes of these species to the overlying waters of the Indian River Lagoon; and (2) to identify the mechanism controlling concentrations of nutrients and trace metals in these muck deposits. Results …

Preliminary Report Results From The City Of Tampa Water Quality, Phytoplankton And Bacteriological Monitoring In The Tampa By-Pass Canal And The Hillsborouhg River: Years 1984-1991, City Of Tampa Department Of Sanitary Sewers

Reports

The information supplied in this report presents monitoring results of water quality, phytoplankton biomass, phytoplankton abundance and taxonomic composition, and bacteriological parameters measured by the City of Tampa Bay Study Group at three stations in the TBC and two stations in the Hillsborough river, upstream of the City of Tampa dam (Figure 1). The time period discussed in this report is from the start of the program in the 1984 through 1991. Results of the measured parameters are presented as annual means, climatological means and summary statistics for the study period.

The Probe, Issue 123 - July 1992

The Probe: Newsletter of the National Animal Damage Control Association

THE PROBE
National Animal Damage Control Association
JULY 1992
Wildlife Damage and Animal Welfare: An Australian Approach
Calendar of Upcoming Events
Tenth Great Plains Wildlife Damage Control Workshop Proceedings.
Animal Damage Control in the News:
Prairie Dog Vacuum Less Than Successful
New Livestock Protection Collar Improves Protection Results
Solano Beach Pet Owners Object to Coyote Trapping
DWRC Assists Developing Countries to Increase Food Availability
Nebraska Sheep Producers Reveal Attitudes Toward Predators
USDA Livestock Depredation Survey
Vermont Citizens Find APHIS/ADC Rabies Hotline Contagious
Utah Man Wants Compensation For Coyote Attack

Nmr Analysis Of Sulfur Compounds In Petroleum, Kanning Wu

Masters Theses & Specialist Projects

The speciation and quantification of organic sulfur forms in fossil fuel is an area of research. This thesis describes an NMR method which offers potential for identifying and possibly quantifying both nonvolatile and volatile sulfur forms in fossil fuels. The method is based on the methylation of sulfur compounds to form methyl sulfonium salts:

RSR + Ch₃I --> (R₂S+-Ch₃)I^~

We propose to apply this chemistry to the analysis of sulfur functions in fossil fuels. The sulfur functions are methylated using ¹³C-enriched methyl iodide. The products are then analyzed by ¹³C NMR …

Black-Tailed Prairie Dog (Cynomys Ludovicianus)

Nebraska Game and Parks Commission: Publications

Black-tailed Prairie Dog(Cynomys ludovicianus):
Description
Distribution and Abundance
Habitat and Home
Habits
Food
Reproduction
Mortality
Importance
Management

Mat Movement In Coastal Louisiana Marshes: Effect Of Salinity And Inundation On Vegetation And Nutrient Levels, Christopher Martin Swarzenski

Biological Sciences Theses & Dissertations

The present research compared and contrasted the physical structure of floating and rooted marshes, their differing responses to open-water salinities and inundation, as well as the nutrient distribution in the porewaters and sediment. The effects of the physical differences in the two marsh types on the ocurrence of the dominant emergent vegetation was discussed.

The main difference in physical structure of the two marsh types was the presence of a mineral, non-buoyant layer at 25-45 cm depth in the rooted marsh, which could serve as an anchor for the overlying highly organic mat layer found in both marsh types. Porewater …

Parallel Computer Needs At Dartmouth College, David Kotz, Fillia Makedon, Matt Bishop, Scot Drysdale, Don Johnson, Takis Metaxas

Computer Science Technical Reports

To determine the need for a parallel computer on campus, a committee of the Graduate Program in Computer Science surveyed selected Dartmouth College faculty and students in December, 1991, and January, 1992. We hope that the information in this report can be used by many groups on campus, including the Computer Science graduate program and DAGS summer institute, Kiewit's NH Supercomputer Initiative, and by numerous researchers hoping to collaborate with people in other disciplines.

We found significant interest in parallel supercomputing on campus. An on-campus parallel supercomputing facility would not only support numerous courses and research projects, but would provide …

Giant Sequoia Insect, Disease, And Ecosystem Interactions, Douglas D. Piirto

Natural Resources Management and Environmental Sciences

Individual trees of giant sequoia (Sequoia gigantea [Lindl.] Decne.) have demonstrated a capacity to attain both a long life and very large size. It is not uncommon to find old-growth giant sequoia trees in their native range that are 1,500 years old and over 15 feet in diameter at breast height. The ability of individual giant sequoia trees to survive over such long periods of time has often been attributed to the species high resistance to disease, insect, and fire damage. Such a statement, however, is a gross oversimplification, given broader ecosystem and temporal interactions. For example, why isn't …

Quantitative Footprinting Analysis Of The Chromomycin A 3 - D N A Interaction, Allison Stankus, Jerry Goodisman, James C. Dabrowiak

Chemistry - All Scholarship

Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)- d(GACTGATGGCCAGACTTG) has been studied using quantitative footprinting methods. Previous NMR studies indicated CHR binds as a dimer in the minor groove. Analysis of autoradiographic spot intensities derived from DNase I cleavage of the 18-mer in the presence of various amounts of CHR revealed that the drug binds as a dimer to the sequence 5’-TGGCCA-3’, 3’-ACCGGT-5’ in the 18-mer with a binding constant of (2.7 f 1.4) X lo7 M-l. Footprinting and fluorescence data indicate that the dimerization constant for the drug in solution is -lo5 M-l. Since it has been suggested that …

Three-Dimensional Solution Structure Of Human Interleukin-4 By Multidimensional Heteronuclear Magnetic Resonance Spectroscopy, Robert Powers, Daniel S. Garrett, Carl J. March, Eric A. Frieden, Angela M. Gronenborn, G. Marius Clore

Department of Chemistry: Faculty Publications

The three-dimensional solution structure of recombinant human interleukin-4, a protein of 133 residues and 15.4 kilodaltons that plays a key role in the immune and inflammatory systems, has been solved by multidimensional heteronuclear magnetic resonance spectroscopy. The structure is dominated by a left-handed four-helix bundle with an unusual topology comprising two overhand connections. The linker elements between the helices are formed by either long loops, small helical turns, or short strands. The overall topology is remarkably similar to that of growth hormone and granulocyte-macrophage colony stimulating factor, despite the absence of any sequence homology, and substantial differences in the relative …