Digital Commons Network^™

New Insights Into Controlling The Functional Properties Of Tin Oxide-Based Materials, Alexandra Kuriganova, Nina Smirnova

Journal of Electrochemistry

Development of methodologies for fabrications of nanostructured materials that provide control over their microstructural features and compositions represents a fundamental step in the advancement of technologies for productions of materials with well-defined functional properties. Pulse electrolysis, a top-down electrochemical approach, has been demonstrated to be a viable method for producing nanostructured materials with a particular efficacy in the synthesis of tin oxides. This method allows for significant control over the composition and shape of the resulting tin oxides particles by modifying the anionic composition of the aqueous electrolyte, obviating the need for additional capping agents in the synthesis process and …

A Cnt Intercalated Co Porphyrin-Based Metal Organic Framework Catalyst For Oxygen Reduction Reaction, Pei-Pei He, Jin-Hua Shi, Xiao-Yu Li, Ming-Jie Liu, Zhou Fang, Jing He, Zhong-Jian Li, Xin-Sheng Peng, Qing-Gang He

Journal of Electrochemistry

The poor electronic conductivity of metal-organic framework (MOF) materials hinders their direct application in the field of electrocatalysis in fuel cells. Herein, we proposed a strategy of embedding carbon nanotubes (CNTs) during the growth process of MOF crystals, synthesizing a metalloporphyrin-based MOF catalyst TCPPCo-MOF-CNT with a unique CNT-intercalated MOF structure. Physical characterization revealed that the CNTs enhance the overall conductivity while retaining the original characteristics of the MOF and metalloporphyrin. Simultaneously, the insertion of CNTs generated adequate mesopores and created a hierarchical porous structure that enhances mass transfer efficiency. X-ray photoelectron spectroscopic analysis confirmed that the C atom in CNT …

Nanostructured Graphitic Carbon Nitride For Photocatalytic And Electrochemical Applications, Muhammad Abdul Qadeer, Iqra Fareed, Asif Hussaine, Muhammad Asim Farid, Sadia Nazir, Faheem K. Butt, Ji-Jun Zou, Muhammad Tahir, Shang-Feng Du

Journal of Electrochemistry

Graphitic carbon nitride (g-C₃N₄) exhibits great mechanical as well as thermal characteristics, making it a valuable material for use in photoelectric conversion devices, an accelerator for synthesis of organic compounds, an electrolyte for fuel cell applications or power sources, and a hydrogen storage substance and a fluorescence detector. It is fabricated using different methods, and there is a variety of morphologies and nanostructures such as zero to three dimensions that have been designed for different purposes. There are many reports about g-C₃N₄ in recent years, but a comprehensive review which covers nanostructure dimensions …

Interaction Of N-Methylmesoporphyrin Ix With A Hybrid Left-/Right-Handed G-Quadruplex Motif From The Promoter Of The Slc2a1 Gene, Paul Seth , '23, Eric Xing , '26, Andrew D. Hendrickson , '27, Kevin Li , '22, R. Monsen, J. B. Chaires, S. Neidle, Liliya A. Yatsunyk

Chemistry & Biochemistry Faculty Works

Left-handed G-quadruplexes (LHG4s) belong to a class of recently discovered noncanonical DNA structures under the larger umbrella of G-quadruplex DNAs (G4s). The biological relevance of these structures and their ability to be targeted with classical G4 ligands is underexplored. Here, we explore whether the putative LHG4 DNA sequence from the SLC2A1 oncogene promoter maintains its left-handed characteristics upon addition of nucleotides in the 5′- and 3′-direction from its genomic context. We also investigate whether this sequence interacts with a well-established G4 binder, N-methylmesoporphyrin IX (NMM). We employed biophysical and X-ray structural studies to address these questions. Our results indicate that …

Biophysical Modeling And Experimental Analysis Of The Dynamics Of C. Elegans Body-Wall Muscle Cells, X. Du, J. Crodelle, Victor J. Barranca, S. Li, Y. Shi, S. Gao, D. Zhou

Mathematics & Statistics Faculty Works

This study combines experimental techniques and mathematical modeling to investigate the dynamics of C. elegans body-wall muscle cells. Specifically, by conducting voltage clamp and mutant experiments, we identify key ion channels, particularly the L-type voltage-gated calcium channel (EGL-19) and potassium channels (SHK-1, SLO-2), which are crucial for generating action potentials. We develop Hodgkin-Huxley-based models for these channels and integrate them to capture the cells’ electrical activity. To ensure the model accurately reflects cellular responses under depolarizing currents, we develop a parallel simulation-based inference method for determining the model’s free parameters. This method performs rapid parallel sampling across high-dimensional parameter spaces, …

Immuno-Bioinformatics Study Of Secondary Metabolites From Watercress Nasturtium Officinale As Immunostimulant In Whiteleg Shrimp Litopenaeus Vannamei By Targeting Peroxinectin And Scavenger Receptor Class B, Qurrota A’Yunin, Muhaimin Rifa’I, Maftuch Maftuch, Yoga Dwi Jatmiko, Muhammad Hermawan Widyananda, Triyanto Triyanto, Muhammad Tegar Handrianto

Karbala International Journal of Modern Science

The viral disease affects the whiteleg shrimp (Litopenaeus vannamei) and causes losses. Immunostimulants are recognized as a more environmentally friendly approach to disease management and antimicrobial properties of aquatic organisms. Watercress (Nasturtium officinale) is an aquatic plant species that contains antioxidant properties that can protect the organism from disease and boost the immune system. Shrimp rely heavily on innate immunity to combat infectious agents due to their absence of adaptive immunity. Peroxinectin and scavenger receptor class B (SRB) play essential roles in the shrimp defense system. This research employs a bioinformatic to assess the immunostimulatory potential …

Quantifying Dust Nutrient Mobility Through An Alpine Watershed, Jeffrey R. Nielson, Janice Brahney

Watershed Sciences Faculty Publications

Dust has the potential to play a significant role in the nutrient dynamics of alpine watersheds with important ecological implications. However, little is known about how dust nutrients circulate through the environment and which watershed characteristics facilitate dust impacts on water quality. This study explored the contribution of dust-deposited nutrients, focusing on a high-elevation Long Term Ecological Research site, where dust samples have been continuously collected since 2017. We incorporated observed dust nutrient compositions, including fractions of inorganic and organic nitrogen and phosphorus, into a popular hydrological model, the Soil and Water Assessment Tool, and ran simulations for 2019–2021. By …

Increasing Atmospheric Evaporative Demand Across The Tibetan Plateau And Implications For Surface Water Resources, Shiqin Xu, Dennis P. Lettenmaier, Tom R. Mcvicar, Pierre Gentine, Hylke E. Beck, Joshua B. Fisher, Zhongbo Yu, Ningpeng Dong, Akash Koppa, Matthew F. Mccabe

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The Tibetan Plateau, known as the “Asian water tower,” is a hotspot for complex hydroclimatic changes. We reveal that the previously decreasing atmospheric evaporative demand (Eo) trend at the end of the 20th century has reversed over the last two decades. Although both wind stilling and solar dimming have persisted, their effects on Eo rates have been overshadowed by increasing air temperatures and decreasing relative humidity, leading to a net rise in Eo for 1980−2015. Using the empirical “top-down” Budyko approach, we estimate that across seven sub-catchments draining the Tibetan Plateau, a 10% increase in annual-averaged …

Breaking Barriers: Investigating Gender Dynamics In Introductory Physics Lab Classes, Bilas Paul, Shantanu Chakraborty, Ganga Sharma

Georgia Journal of Science

The persistent underrepresentation of women and other gender minorities in physical science fields has been an ongoing concern. This study investigates gender dynamics in introductory physics laboratory courses, focusing on whether students of different gender identities exhibit equal inclination and confidence in conducting lab experiments, and whether they face barriers that impact their participation. Conducted across three institutions and involving non-physics STEM students enrolled in algebra-based and calculus-based physics courses, the study found mixed results, with two institutions showing no significant gender-based differences in participation levels during lab activities, while one institution demonstrated significant differences. Chi-square tests revealed no significant …

Ethnic Disparities In Hba1c And Hypoglycemia Among Youth With Type 1 Diabetes: Beyond Access To Technology, Social Deprivation And Mean Blood Glucose, J. Pemberton, Zhide Fang, Stuart Chalew, S. Uday

School of Public Health Faculty Publications

Introduction The UK national pediatric diabetes audit reports higher HbA1c for children and young people (CYP) with type 1 diabetes (T1D) of Black ethnicity compared with White counterparts. This is presumably related to higher mean blood glucose (MBG) due to lower socioeconomic status (SES) and less access to technology. We aimed to determine if HbA1c ethnic disparity persists after accounting for the above variables. Research design and methods A retrospective analysis of participants who received structured education in continuous glucose monitoring (CGM) use was conducted at a tertiary center. HbA1c was paired with glucose metrics from 90-day CGM data. The …

Single-Molecule Dna-Flow Stretching Assay As A Versatile Hybrid Tool For Investigating Dna-Protein Interactions, Sadaf Shehzad, Hyeongjun Kim

Physics & Astronomy Faculty Publications

Single-molecule techniques allow researchers to investigate individual molecules and obtain unprecedented details of the heterogeneous nature of biological entities. They play instrumental roles in studying DNA-protein interactions due to the ability to visualize DNA or proteins and to manipulate individual DNA molecules by applying force or torque. Here, we describe single-molecule DNA-flow stretching assays as hybrid tools that combine forces with fluorescence. We also review how widely these assays are utilized in elucidating working mechanisms of DNA-binding proteins. Additionally, we provide a brief explanation of various efforts to prepare DNA substrates with desired internal protein-binding sequences. More complicated needs for …

Development And Validation Of Rapid Edna Detection Method For Yellow Mud Turtle, Kinosternon Flavescens: A Field Study In South Texas, Usa, Sakib Tahmid Rishan, Richard Kline, Md Saydur Rahman

School of Earth, Environmental, & Marine Sciences Faculty Publications

The conservation of freshwater turtle species depends on precise and effective monitoring techniques. Environmental DNA (eDNA) analysis is a potential method for identifying cryptic and elusive turtle species in aquatic ecosystems. eDNA analysis can help to identify key regions for conservation efforts and monitor changes in population levels over time. This study aims to evaluate the effectiveness of a rapid eDNA detection method for the yellow mud turtle (Kinosternon flavescens, an indicator species that is endangered in some states in the USA), which inhabits local oxbow lakes (e.g., resacas) in Cameron County, South Texas. A species-specific nested PCR …

Chemistry Of Metalloguanines: An Overview Of Their Synthesis Routes And Their Implementations For The Period 2000-2024, Farah Saadoon Jaafar, Mahasin F. Alias

Karbala International Journal of Modern Science

Guanine has a variety of roles in chemistry, from its basic function in the storing and transferring genetic information to its usages in synthetic chemistry and other fields. Because of its distinct structure and biological importance, it is a fundamental component of contemporary study in organic chemistry and molecular biology.

In this review, we focused on covering the synthetic pathways of various derivatives of guanine from the year 2000 until the present. As a result of the guanine molecule containing multiple functional groups, this gives us the ability to prepare several guanines such as O⁶-alkylating guanines, O^{6 …}

Native Organizations Fill Gaps In Federal Support For Tribal Renewable Energy, Healing Native Communities, Corrie Grosse, Dawn D. Davis, Robert Blake, Chéri Smith

Environmental Studies Faculty Publications

No abstract provided.

Community-Centered Climate Resilience Strategies For Agriculture In Chile's Los Lagos, Asa B. Stone, Cristián Kremer F., Mark C. Stone

Cornhusker Economics

Community-centered approaches using the SETS framework facilitate equitable community-academia partnerships that co-create climate resilience strategies. By integrating local insights with academic research ethically and equitably, PLL aims to develop evidence-based, culturally relevant, and technologically feasible strategies. This holistic approach not only enhances community resilience but also ensures the sustainable management of water resources in the Los Lagos Region.

Climate Influence On Both Spatial And Temporal Patterns Of Rocky Intertidal Community Structure In San Diego, California, Emily Jenkins

Theses

The rocky intertidal ecosystem, the coastal area between high and low tides, serves as a critical buffer between land and sea. This ecosystem supports a diverse array of species and provides rich opportunities for exploration and education. With the influence of climate change, this ecosystem faces significant shifts in species distribution, resilience and recovery from environmental disturbances, as well as habitat loss (Jackson & McIlvenny 2011; Jueterbock et al. 2013; Menge & Gravem 2022; Rilov et al. 2021; Sagarin & Barry 1999). Between 2014-2016, climate anomalies El Niño (ENSO) and the Pacific Decadal Oscillation (PDO) combined to create a period …

Milk Yield Of Cows With Different Genetic Potential On Grass And Grass-Legume Tropical Pastures, C E. Lascano, P Avila

IGC Proceedings (1977-2023)

A grazing experiment was carried out at CIAT's Quilichao research station to assess the effect of legumes selected for acid soils on milk production of cows with different genetic potential. Pastures of Brahiaria dictyoneura cv. Llanero and Andropogen gayanus cv. Carimagua l, alone and in association with Centrosema acutifolium (CIAT 5568) and C. macrocarpum (CJAT 5713), were grazed by Holstein and Zebu crossbred cows using a Latin square crossover design. Fat corrected milk (FCM) yield of Holstein cows was 17 and 19% higher in the associations of B. dictyoneura and A. gayanus, respectively, than in the grass-only pastures. With the …

Fattening Heifers On Nitrogen Fertilized And Irrigated Pastures: A Productive And Sustainable System For The Tropics?, F Champanhet, M Mahieu

IGC Proceedings (1977-2023)

Beef production from nitrogen-fertilised and irrigated pastures was studied for 4,S years on vertisols, South Martinique (rainfall 1400 mm). Digitaria decumbens (pangola) pastures received 230��7S-ISO kg/ha/year N-P·K and were rotationally grazed (S days grazed, 30 days rested) all year round with a fixed stocking rate (6.6 heifers/ha). Mean live-weight gains were: 1121 kg/ha/year and 170 kg/head/ year. Pangola showed good persistence and cover at the end of the experiment exceeded 9S%, It is concluded that heifers fattening on nitrogen-fertilised pastures is a productive and stable system.

Comparative Use Of Digitaria Decumbens And Cynodon Nlemfuensis By Local Suckling Ewes In Martinque (Fwi), Maryline Boval, Gisele Alexandre, M Mahieu, P Cruz, M Meuret

IGC Proceedings (1977-2023)

Pastures of Digitaria decumbens (Dd) and Cynodon nlemfuensis (Cn) were each grazed by 64 native suckling ewes ( 40 ewes/ha) in Martinique. Forage characteristics, ewe feeding behaviour and lamb growth were studied. The available herbage mass (kg DMJLW0-75/day) was lower for the Dd pastures than for the Cn pastures: 295 v. 355 for the leaves (P<0.05) and 820 v, 1360 for the stems (P<0.01), respectively. There was no difference in the diurnal activities (grazing, ruminating) between the ewes in the 2 groups. Number of bites/min was higher on Dd pasture than that for Cn: 31 v. 26 (P<0.001), Pre­weaning daily weight gain (g/day) was higher for the Dd lambs group than for Cn: 170 v. 140 (P<0.01). The lamb mortality rate(%) was respectively: 15 v. 27 (P<0.05). Pasture management problems are discussed.

Stockpiling Of Brachiaria Decumbens For Steer Maintenance During The Dry Season, Esther G. Cardoso, V.P B. Euclides, J.M Da Silva

IGC Proceedings (1977-2023)

A Brachiaria decumbens pasture was evaluated in a factorial experiment (2 stocking rates and 2 pasture managements). Eight steers averaging 370 kg live weight (LW) were allocated to the paddocks at the beginning of the wet season ( 1 December). For the deferred management treatment, half of the area was closed off at the end of the rainy season ( 15 March to 15 June) for utilisation as stockpiled feed during the dry season (15 June to 30 October). B. decumbems was capable of fattening Nellore steers during the rainy ·season, but maintained steer live weight (LW) only in the …

Seasonal Changes In The Chemical Composition Of Cultivated Tropical Grasses In The Savannas Of Brazil, M.C M. Macedo, V.P B. Euclides, M.P De Oliveira

IGC Proceedings (1977-2023)

Five tropical grasses; Brachiaria brizantha cv. Marandu, B, decumbens cv. Basilisk, Panicum maximum cv. Coloniilo, P. maximum cv, Tobiatil, P. maximum cv. Tanzania-I, were planted on a dark red latosol (Oxisol) and grazed for 3 rainy and 3 dry seasons . The original savanna vegetation was removed, the soil cultivated and fertilised with 1000 kg/ha of dolomitic limestone, 350 kg/ha of single superphosphate, 100 kg/ha of KCI and 40 kg/ha of fritted traced elements. Treatments were arranged in a RCB design with 3 replicates. Paddocks were 1.5 ha. Grazing pressure was adjusted to approximately 8 or 15 kg of …

Evaluation Of Panicum Maximum Cultivars Under Grazing, V.P B. Euclides, M.C M. Macedo, A Vieira, M P. Oliveira

IGC Proceedings (1977-2023)

A grazing trial was conducted in Brazil, for a 3-year period, to study animal production from Panicum maximum cultivars (Coloniao, Tobiatii and Tanz5nia-1). Three 1.5-hn paddocks of each cultivar were stocked continuously with 3 steers, and additional grazers were added or removed as required to ensure similar grazing pressure in each treatment. Steers on Tanzania-I had a superior average daily gain (ADG) to those on Tobiatii and Coloniffo. However, the lack of difference in carrying capacity between Tanzdnin and Tobiatil pastures resulted in similar live-weight gain (LWG)/ha. The ADG and LWG/ ha of Coloniilo were significantly lower than the other …

Evaluation Of Brachiaria Decumbens And Brachiaria Brizantha Under Grazing, V.P B. Euclides, A H. Zimmer, M P. Oliveria

IGC Proceedings (1977-2023)

A 3-year grazing trial was conducted in Brazil, to relate changes in sward characteristics of B. decumbens and B. brizantha to animal live-weight gain (LWG). Three 1.5-ha paddocks of each grass species were stocked continuously with 3 steers, and additional grazers were added or removed as required to ensure similar grazing pressure in each treatment. Despite similar (P>0.85) annual average daily gains (ADO) of steers grazing both species, the ADO of steers grazing B. brizantha pasture was significantly (P<0.05) higher during the wet season, and significantly (P<0.05) lower in the dry period, when compared with those on B. decumbens. Annual LWG per area of both species were also similar (P>0.85). The best predictor of ADO was …

Effects Of Grain Supplement For Steers Grazing Stargrass Pastures On Subsequent Feedlot Gains And Carcass Characteristics, G.M.J Horton, W D. Pitman, E M. Hodges

IGC Proceedings (1977-2023)

Performance of steers (181 kg) grazing slargrass pastures was evaluated al 2 levels of grain supplementation in comparison with unsupplemenled cattle and with steers in drylot fed a high-roughage diet. Supplements during the 140-day winter period were fed at 1.0 (moderate) and 1.5% body weight (BW)lday (high), followed by 0.40 kg (moderate) and 0.5% BW/day (high) during the 98-day spring grazing period. Gains during a subsequent 100-dny, high-energy feeding period and resulting carcass characteristics were also compared. Gains during !he grazing period did not differ between !he 2 levels of supplement. Daily rate of gain of unsupplemented cattle (0.44 kg) …

Large-Scale Property Demonstrations Of The Effect Of Sexa Stylo And Phosphorus On Beef Cattle Growth In Central Queensland, Australia, C H. Middleton, K J. Murphy, G W. Blight, Vicki-Lee Hansen

IGC Proceedings (1977-2023)

Seca style (Stylosanthes scabra) was oversown into black speargrass (Heteropogon comortus)•dominant pasture after a burn at 2 sites in central Queensland, and the pasture was continuously grazed. At one site with <5 ppm bicarbonate-extractable soil P, Seca growth was poor and gave only a small animal growth response (18 kg/steer/year) over native pasture. With the addition of superphosphate (27 kg P/ha) the Seca-native grass produced an additional live-weight gain of 70 kg/ steer/year over the native pasture control. At a second site with 6-8 ppm available soil P, Seca grew well, and with a P supplement given to steers, live-weight gains were 40 kg/steer/year more than for P­supplemented animals grazing native pasture. Oversowing Seca into native pasture with P as fertiliser or a supplement can dramatically improve animal performance. The project was conducted on private properties and was aimed to focus producer attention on this technology, which is suited to large areas of northern Australia.

Improving Hill Agriculture Systems In Nepal: A Case Study, B N. Tiwari, J B. Abington

IGC Proceedings (1977-2023)

The Kingdom of Nepal is characterised by complex, diverse smallholder farming systems in which crops, livestock and forest resources are all interlinked. Traditional feeding practices throughout the hills are coming under severe pressure due to the high density of livestock. Consequently, acute shortages of forage are e, experienced, especially during the dry winter season. A case study is presented In which the integration of forage management into the whole farming system is practised, User groups are improving community grazing lands by the introduction of more productive herbage and fodder tree species. Fodder is sold to livestock farmers, animal management is …

Development Of Forage Systems For Dairying In Subtropical Australia, R J. Moss, K F. Lowe

IGC Proceedings (1977-2023)

The development of productive dairy farm systems integrating irrigated temperate pastures with rain-grown tropical pastures in subtropical Australia is described, Milk production increased from 2976-34211/cow (P<0,01) with increased temperate pasture allowance from 0. I to 0.2 ha/cow, and this increase was similar for nitrogen­fertilised ryegrass or clover-ryegrass mixtures, Temperate pastures provided cows with a high protein diet (20-30% crude protein}, enabling energy-rich grain and molasses concentrates to be efficiently used, Increasing supplementation to S kg grain/cow day increased milk yields to S109 I/lactation. Nitrogen fertilisation of perennial tropical grasses enabled higher stocking rates to be used and, together with the increases in production/cow, increased farm output from 464S I/ha to 7300 I/ha.

Use Of Blood Urea Nitrogen Concentration To Predict Response To Protein Or Energy Supplementation In Grazing Cattle, A C. Hammond, W E. Kunkle, D B. Bates, L E. Sollenberger

IGC Proceedings (1977-2023)

In healthy ruminants, blood urea nitrogen (BUN) concentrations are positively correlated with ruminal ammonia nitrogen concentrations and are indicative of the protein to energy ratio in the diet. Our objective was to determine whether BUN could predict the biological response (average daily gain, ADG) to protein or energy supplementation in free­grazing steers and heifers on tropical grass pastures, Change in ADO (-0.0S to 0.30 kg) due to protein supplementation was linearly related to BUN concentration (6,2-15,5 mg/di) in control cattle (P<0.05, slope= 0.023, r = 0.69, s = 0.07 kg). BUN concentrations of 9-12 mg/di were a transition range t��tow which ADG response to protein supplementation was greater (P«J.05) and above which ADG response was lesser (P<0, l 0) than the response within thls range. Change in ADO due to energy supplementation was not related to BUN in control cattle, but the range of BUN in our trials was limited to concentrations above 9 mg/di. Concentration of BUN may be a useful tool for predicting biological response to protein supplementation of grazing beef cattle.

Utilization Of Faba Bean (Vicia Faba L.) Fodder And Silage In Ruminants, P C. Gupta, M A. Akbar

IGC Proceedings (1977-2023)

Two feeding experiments were conducted to find out the nutritive value of faba bean (Vicia faba L.) green fodder and silage, In the first experiment, mixtures of faba bean and oat (Avena sativa) green fodders in proportions of 1:1, 1:2 and 2:1, respectively, were fed to lambs randomly divided in to 3 groups of 6 each for 50 days, The DM intake of lambs, change in their body weights and digestibility co-efficients of various nutrients did not differ (P>0.05) among the 3 groups. However, the values of DCP in different rations varied significantly (P<0.05) but not that of TDN. In the second experiment, pure faba bean was ensiled for 50 days and offered to sheep, which maintained their body weights. It was concluded that effective utilisation of green faba bean in sheep could be achieved either by ensiling or by mixing it with other forages like oat. Feeding of faba bean and oat mixture al 2:1 ratio gave optimum growth in lambs,

Utilization Of Leucaena Leucophala As Fodder For Buffaloes, P C. Gupta, M A. Akbar

IGC Proceedings (1977-2023)

A feeding trial was conducted for 122 days to assess the effect of feeding Leucaena leucophala day on growth rate, utilisation of nutrients, and its toxic effects, if any, in 15 Murrah male buffalo calves about 1 year old. Calves in control group (Tl) were maintained on concentrate mixture and ad lib. wheat straw. The protein of concentrate mixture was replaced at 25% and 50% levels by leucaena protein in the rations of group T2 and T3, respectively, Leucaena hay was isonitrogenous (CP about 19%). The mimosine, tannin and 6-carotene in leucnena hay were 2.50%, 7.33 mg/g and 504.24 mg/kg, …