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Articles 4921 - 4950 of 78652
Full-Text Articles in Entire DC Network
Food Insecurity In Latin America: Climate Change, Industrial Agriculture, And The Case For Regenerative Solutions, Leila Hernandez-Webster
Food Insecurity In Latin America: Climate Change, Industrial Agriculture, And The Case For Regenerative Solutions, Leila Hernandez-Webster
CISLA Senior Integrative Projects
This study critically analyzes the nexus between climate change and food insecurity in Latin America, with a particular focus on Costa Rica’s agricultural sector. It interrogates how industrial agricultural practices, deforestation, and trade liberalization have contributed to environmental degradation and heightened socio-economic vulnerabilities, especially among smallholder farmers. Through the lens of political ecology, this research situates these dynamics within the broader context of neoliberal and neo-extractivist development paradigms, which have systematically eroded food sovereignty. This study also evaluates agro-ecological responses, particularly agroforestry, as viable strategies for promoting ecological resilience and socio-economic equity. In doing so, it underscores the imperative of …
Changes In The Western Flank Of The North Atlantic Subtropical High Since 1140 Ce: Extremes, Drivers, And Hydroclimatic Patterns, Joshua C. Bregy, Justin T. Maxwell, Scott M. Robeson, Grant L. Harley, Valerie Trouet
Changes In The Western Flank Of The North Atlantic Subtropical High Since 1140 Ce: Extremes, Drivers, And Hydroclimatic Patterns, Joshua C. Bregy, Justin T. Maxwell, Scott M. Robeson, Grant L. Harley, Valerie Trouet
Publications
Summer circulation and moisture patterns in the Southeast United States are controlled by the position of the North Atlantic subtropical high. In a warming climate, the subtropical high is projected to strengthen and expand west, but there remains uncertainty regarding its variability and linkages to natural drivers. Here, we use a tree-ring network across the Southeast United States to reconstruct the relative intensity of the pressure gradient across the subtropical high’s western flank over the past 870 years. Variations in the flank’s position and the pressure gradient have been a major driver of the hydroclimate—including creating a Southeast-Caribbean moisture dipole—since …
Primates From Senèze, Eric Delson
Primates From Senèze, Eric Delson
Publications and Research
Senèze has yielded two primate fossils, neither from the recent field work. A partial ulna can be identified as cf. Macaca sylvanus. A nearly complete female skull is the holotype of Dolichopithecus arvernensis Depéret, 1928, now included in Paradolichopithecus. Emended and differential diagnoses are provided for this genus and species for the first time, and the Senèze skull is described and briefly compared to those of other species of the genus. Dental and cranial measurements are provided, along with comparative data. The macaque and Paradolichopithecus are moderately to highly terrestrial, respectively, and they suggest a woodland to more open …
Application Of Analytical Methodologies To The Analysis Of Food Flavoring Agents: Part Ii, James Daniel, Kexin Li, Anaaya Monroe, Melissa C. Rhoten
Application Of Analytical Methodologies To The Analysis Of Food Flavoring Agents: Part Ii, James Daniel, Kexin Li, Anaaya Monroe, Melissa C. Rhoten
Spring Showcase for Research and Creative Inquiry
No abstract provided.
Sheep Production, Profitability And Greenhouse Gas Emission Of Mixed Farming Systems In Western Australia's Wheatbelt, Tafesse Estifanos, Amir Abadi Abadi Ghadim, Kate Pritchett, Christophe D'Abbadie, Johnny Machon, Paul Mattingley, Bradley Plunkett, Gus Manatsa, Kerrine Blenkinsop, Ross Kingwell, Clinton K. Revell Dr
Sheep Production, Profitability And Greenhouse Gas Emission Of Mixed Farming Systems In Western Australia's Wheatbelt, Tafesse Estifanos, Amir Abadi Abadi Ghadim, Kate Pritchett, Christophe D'Abbadie, Johnny Machon, Paul Mattingley, Bradley Plunkett, Gus Manatsa, Kerrine Blenkinsop, Ross Kingwell, Clinton K. Revell Dr
Animal production and livestock research reports
The south-western agricultural area of Western Australia is a vital contributor to Australia's agricultural output. It generates over $10 billion annually in agricultural production from 2017–18 to 2021–22 and more than $8 billion in export value over the same period. Mixed farming systems characterise the region, producing broadacre crops (primarily wheat, barley, and canola) and livestock. The region's livestock industry is dominated by sheep production for meat and wool, and the state’s share of the national sheep flock in 2020–21 was at 18%.
This report provides a comprehensive review and industry analysis of sheep production in WA, its economic significance, …
Spiritual Geographies And Living Ritual: Misali Island’S Caves In Swahili Religious Life In Zanzibar, Everett Gordon
Spiritual Geographies And Living Ritual: Misali Island’S Caves In Swahili Religious Life In Zanzibar, Everett Gordon
Tanzania-Zanzibar: Coastal Ecology and Natural Resource Management
Caves along the East African Swahili coast are more than geological formations—they are spiritual landscapes where the boundaries between visible and invisible worlds collapse. This paper explores the ritual and religious significance of three caves on Misali Island, a small coral rag island off the coast of Pemba in the Zanzibar Archipelago. Drawing on vegetation surveys and three weeks of ethnographic fieldwork, including interviews with local fishermen, waganga (healers), and marine rangers, this study investigates how these caves serve as sites of prayer, healing, and spirit veneration. It argues that the practices performed within and around these caves exemplify the …
Baobab Beach Profile: Erosion Of North-Northeast Misali Island, Elizabeth Waterfall
Baobab Beach Profile: Erosion Of North-Northeast Misali Island, Elizabeth Waterfall
Tanzania-Zanzibar: Coastal Ecology and Natural Resource Management
This study investigates the patterns and drivers of coastal erosion on Misali Island,located off the coast of Pemba Island in the Zanzibar Archipelago. Fieldwork was conducted over a 28-day period, focusing on four beachfront locations along Baobab Beach. Measurements included strike and dip, beach crest-to-toe distance (m), beach step height (cm), sand composition and sand color, velocity, coral composition, and a rate of sediment accumulated per day. These data, combined with qualitative observations, reveal spatial variability in sediment deposition and erosion severity across the island’s coastline. Results suggest that reduced coral cover and increased exposure to wave energy are contributing …
Agricultural Innovation To Climate Change In Northwest Pemba Island, Margaret Horn
Agricultural Innovation To Climate Change In Northwest Pemba Island, Margaret Horn
Tanzania-Zanzibar: Coastal Ecology and Natural Resource Management
This study investigates how climate change is reshaping agriculture and food security on Pemba Island, Zanzibar, a region historically known as “The Green Island” for its rich biodiversity and agricultural productivity. Through semi-structured interviews with 11 local farmers and extended participant observation at the farm of government official Amour Juma Mohammed, the research reveals that increasingly erratic rainfall, rising temperatures, and prolonged droughts are disrupting traditional planting cycles and reducing crop yields. Farmers report the near disappearance of the vuli rainy season and increasingly severe weather during masika, leading to both water scarcity and crop damage. In response, many have …
Resurrecting The Past: Engineering Ancestral Enzymes For Modern Bacterial Applications, Gregory M. Gladkowski Iii, Masa Watanabe
Resurrecting The Past: Engineering Ancestral Enzymes For Modern Bacterial Applications, Gregory M. Gladkowski Iii, Masa Watanabe
SACAD: Scholarly Activities
Ancestral Sequence Reconstruction (ASR) is a computational technique that infers and resurrects ancient protein sequences to explore molecular evolution and enable protein engineering. By integrating phylogenetics and statistical modeling, ASR produces stable, mutation-tolerant enzymes ideal for directed evolution. This poster presents a workflow for reconstructing enzymes from pathogenic bacteria and highlights ASR’s value in uncovering novel functions and advancing applications in drug discovery and synthetic biology.
Building Bridges Of Knowledge, Cuny Innovative Teaching Academy (Cita)
Building Bridges Of Knowledge, Cuny Innovative Teaching Academy (Cita)
Open Educational Resources
Building Bridge of Knowledge (BBK) is a CUNY-wide faculty fellowship project, aiming to help students use generative artificial intelligence (AI) tools ethically, responsibly, and effectively. Funded by Lumina Foundation in 2024-25, the project was executed by the CUNY Innovative Teaching Academy (CITA) of the Office of Faculty Affairs at CUNY Central Office, with 25 Faculty Fellows in five disciplinary areas (Education, Health, Humanities, Social Sciences, and STEM) chosen from across CUNY’s senior and community colleges. Each disciplinary cohort was led by a CUNY faculty member with expertise in the use of AI. The final materials, described and linked in this …
A Personalized And Adaptive Distribution Classification Of Actigraphy Segments Into Sleep-Wake States, Austin G. Vandegriffe, V. A. Samaranayake, Matthew S. Thimgan
A Personalized And Adaptive Distribution Classification Of Actigraphy Segments Into Sleep-Wake States, Austin G. Vandegriffe, V. A. Samaranayake, Matthew S. Thimgan
Research Data
Wearable actimeters have the potential to greatly improve our understanding sleep in natural environments and in long-term experiments. Current technologies have served the sleep community well, but they have known weaknesses that introduce errors that can compromise reliable and relevant clinical and research sleep and wakefulness profiles from these data. Newer data collection technologies, such as microelectromechanical systems (MEMS), offer opportunities to gather movement data in different forms and at higher frequencies, making new analytical methods possible and potentially advantageous.
We have developed a novel statistical algorithm, called the Wasserstein Algorithm for Classifying Sleep and Wakefulness (WACSAW), that is based …
Chalmers Library Research Award Winner 1 2025, Tasnim Islam Orco
Chalmers Library Research Award Winner 1 2025, Tasnim Islam Orco
Chalmers Library Research Award
No abstract provided.
Understanding The Growth Mechanism Of Thiol-Conjugated Au25 Cluster, Chunhui Liu, Haiying He, Ravindra Pandey, Shashi P. Karna
Understanding The Growth Mechanism Of Thiol-Conjugated Au25 Cluster, Chunhui Liu, Haiying He, Ravindra Pandey, Shashi P. Karna
Michigan Tech Publications
The synthesis of ligand-conjugated gold nanoclusters has attracted significant attention due to its ability to achieve precise control over cluster size selectivity. Among these, Au25(SR)18-, where R represents an alkyl group, is one of the earliest being synthesized with a very high yield, although its growth mechanism is yet to be fully understood. Using density functional theory, we present the results of a theoretical investigation on the growth process of Au25(SR)18-, beginning from Au13(SR)12-. Our findings indicate that the sulfur atoms in the core structure of Au13 …
Streamlining Wildlife Regulation, Robert L. Fischman
Streamlining Wildlife Regulation, Robert L. Fischman
Michigan Journal of Environmental & Administrative Law
A recent burst of revisions in the bedrock regulations governing projects that may adversely affect wildlife represents a generational shift in policy. Streamlining federal decision -making drove much of this reform. Streamlining offers a path to address climate change without abandoning a longstanding commitment to wildlife conservation. The conservation community recognizes the need to build new infrastructure to reduce the rate of climate change through air emissions. The most urgent priority is decarbonizing the electrical ~rid through renewable energy generation and a better transmission network. Adaptation to the "new abnormal" of climate-driven environmental disruption demands federal approvals for a host …
Enhancing Metacognitive Competencies Through Human-Centered Ai: The Role Of Custom-Trained Intelligent Agents In Workforce Upskilling, James Hutson
Faculty Scholarship
This editorial examines the integration of human-computer intelligent interaction (HCII), specifically through human-centered artificial intelligence (AI) and custom-trained intelligent agents, to foster metacognitive competencies critical for workforce upskilling. With 59% of the workforce projected to require substantial upskilling by 2030, developing personalized AI models tailored to individual cognitive and learning profiles presents an innovative pathway. These custom-trained agents leverage human-computer interaction (HCI) technologies and machine learning methodologies to enhance understanding of one’s own learning processes-metacognition-thus empowering individuals to optimize their future learning and adaptability. This approach not only enhances the individual’s ability to engage effectively with complex tasks in the …
Water Current, Volume 57, No. 1, Spring 2025
Water Current, Volume 57, No. 1, Spring 2025
Water Current Newsletter
No abstract provided.
Kinetic Control And Trapping In The Supramolecular Polymerization Of M-Terphenyl Bis-Urea Macrocycles, Sophya V. Garashchuk, Gamage Isuri P. Wijesekera, Isabella G. Rushton, Vaibhavi A. Samant, Fahidat A. Gbadamosi, Dr. Md Faizul Islam, Sophya V. Garashchuk, Prof. Dr. Linda S. Shimizu
Kinetic Control And Trapping In The Supramolecular Polymerization Of M-Terphenyl Bis-Urea Macrocycles, Sophya V. Garashchuk, Gamage Isuri P. Wijesekera, Isabella G. Rushton, Vaibhavi A. Samant, Fahidat A. Gbadamosi, Dr. Md Faizul Islam, Sophya V. Garashchuk, Prof. Dr. Linda S. Shimizu
Faculty Publications
Herein, we examine pathway complexity in the supramolecular polymerization of a novel m-terphenyl bis-urea macrocycle. Designed to induce kinetically metastable states, the macrocycle‘s concentration-dependent aggregation was studied via 1H NMR and IR spectroscopy in THF and CHCl₃. Temperature-dependent UV-Vis spectroscopy in water/THF revealed a cooperative nucleation-growth mechanism, indicated by a shift in λmax to longer wavelengths upon cooling. Morphological studies using DLS, AFM, and SEM demonstrated fibrous aggregate formation. Thermal hysteresis observed in assembly-disassembly cycles indicated kinetically trapped species, with cooling governed by kinetic control and heating by thermodynamic processes. Deviations in ΔH values during cooling, compared to van′t …
How The Biotic And Abiotic Factors In The Belle Forest Cave And Arboretum Soil Dictates Tree Species Prevalence, Amya A. Bingham
How The Biotic And Abiotic Factors In The Belle Forest Cave And Arboretum Soil Dictates Tree Species Prevalence, Amya A. Bingham
SPARK Symposium Presentations
A plethora of factors in the soil impact a plant’s health, including the presence of different biotic and abiotic factors. These include things like temperature, elevation, pH, microbial respiration and the presence of water, clay, loam, silt, sand, nitrogen, potassium, and phosphorus. Different soils will have varying amounts of these, which may dictate what is able to grow in them, and therefore also the surrounding area. This research project explores how the aforementioned biotic and abiotic factors affect the soil at the Belle Forest Cave and Arboretum. Three samples will be taken from four spots along a transect at this …
Investigating Mercury Contamination In Urban Fish: Atomic Absorption Spectroscopy And Histological Analysis For Environmental And Human Health, Indy L. Cockrill, Ross Andrew
Investigating Mercury Contamination In Urban Fish: Atomic Absorption Spectroscopy And Histological Analysis For Environmental And Human Health, Indy L. Cockrill, Ross Andrew
SPARK Symposium Presentations
Mercury contamination in aquatic ecosystems is a pressing environmental and public health concern, particularly in urban areas where industrial runoff and wastewater contribute to heavy metal accumulation. This study investigates mercury presence in fish from Richland Creek in Nashville, TN, by analyzing histological changes in liver and muscle tissues. Mercury bioaccumulates in aquatic food chains, potentially leading to physiological damage in fish and posing health risks to humans who consume contaminated seafood. While prior research has established mercury’s toxic effects, there is a need for localized studies assessing its presence in urban waterways. The hope is to find histological abnormalities …
Microbial Distortion? Impacts Of Delayed Preservation On Microbiome Diversity And Composition In A Marine Invertebrate, Brenna Hutchings, Susanna López-Legentil, Lauren Stefaniak, Marie Nydam, Patrick M. Erwin
Microbial Distortion? Impacts Of Delayed Preservation On Microbiome Diversity And Composition In A Marine Invertebrate, Brenna Hutchings, Susanna López-Legentil, Lauren Stefaniak, Marie Nydam, Patrick M. Erwin
Marine Science
Field collections of marine invertebrates are often accompanied by delays in preservation, which may impact microbiome composition. Here, we tested the effects of delayed preservation and relaxation methods on microbiome diversity and composition in the colonial ascidian Trididemnum solidum using 16S rRNA amplicon sequencing. Replicate samples collected from Belizean reefs were either (1) immediately preserved in ethanol (“control”), (2) held in ambient seawater for 3 h before preservation (“SW”), or (3) held in ambient seawater with menthol (a common pre-preservation relaxation technique for ascidian identification) for 3 h before preservation (“SW + M”). All T. solidum microbiomes were different from …
Innovative Soil Classification Approach For Achieving Global Biodiversity Framework Utilizing Integrated Data Fusion Of Emit And Multispectral Satellite Observations: Case Study Of Imam Turki Bin Abdullah Royal Reserve, Kingdom Of Saudi Arabia, Hesham Morgan, Ali Elgendy, Surendra Maharjan, Wenzhao Li, Tamer Ismail, Yehya Kh. Shehadeh, Ahmed Elgharib, Ahmed Abdullah Al-Dughairi, Ali El Muburak, Khaled Allam Harhash, Hesham El-Askary
Innovative Soil Classification Approach For Achieving Global Biodiversity Framework Utilizing Integrated Data Fusion Of Emit And Multispectral Satellite Observations: Case Study Of Imam Turki Bin Abdullah Royal Reserve, Kingdom Of Saudi Arabia, Hesham Morgan, Ali Elgendy, Surendra Maharjan, Wenzhao Li, Tamer Ismail, Yehya Kh. Shehadeh, Ahmed Elgharib, Ahmed Abdullah Al-Dughairi, Ali El Muburak, Khaled Allam Harhash, Hesham El-Askary
Mathematics, Physics, and Computer Science Faculty Articles and Research
Soil classification is essential for sustainable land management, ecological conservation, and combating desertification, particularly in arid and semi-arid regions. This study integrates hyperspectral data from the Earth Surface Mineral Dust Source Investigation (EMIT) and multispectral imagery from Sentinel-2 to achieve accurate soil classification for the Imam Turki bin Abdullah Royal Reserve (ITBA) in Saudi Arabia. Using advanced Machine Learning (ML) techniques, including Extreme Gradient Boosting (XGBoost), the study highlights the power of data fusion in addressing the limitations of standalone remote sensing methods. The integration of hyperspectral and multispectral data combines the spectral richness of hyperspectral imaging with the spatial …
Drawing Lines In The Sand: Armed Conflict, Climate Damage And Setbacks To Climate Action, Beatrice L. Hamilton
Drawing Lines In The Sand: Armed Conflict, Climate Damage And Setbacks To Climate Action, Beatrice L. Hamilton
Michigan Journal of Environmental & Administrative Law
Armed conflicts have a significant and detrimental effect on the climate system, with considerable implications for efforts to mitigate and adapt to climate change and the civilian population. Yet the environmental provisions under the law of armed conflict (LOAC), drafted some half a century ago, struggle to adequately address these concerns. This article assesses the capacity of LOAC to address conflict-driven climate damage and its cascading impacts by characterizing these harms as environmental damage and as injury to civilians. The analysis highlights opportunities for broadening LOA C's scope and offers recommendations to enhance its capacity to address climate damage and …
Click-Based Synthesis Of Hemibastadin Derivatives And Their Applications As Antimicrobial And Biofilm Inhibiting Agents, James B. Daniel Iii
Click-Based Synthesis Of Hemibastadin Derivatives And Their Applications As Antimicrobial And Biofilm Inhibiting Agents, James B. Daniel Iii
Longwood Senior Thesis Proposal
Antimicrobial agents are one of the most important tools used in the medical industry. With the ever growing numbers of antibiotic resistant bacteria, new strategies have been developed to combat them, one of which being biofilm inhibiting agents. A class of molecules called hemibastadins, first discovered from sea sponges, have been of recent interest in the field. Building from previous work done in this lab on hemibastadins, a hemibastadin derivative containing a triazole in place of the oxime has been identified as a potential antibiofilm agent. This work will target a derivative that will more closely mimic the hemibastadins found …
Nanosecond Pulsed Electric Field And Plasma Jets For Cancer Therapy, Edwin Ayobami Oshin
Nanosecond Pulsed Electric Field And Plasma Jets For Cancer Therapy, Edwin Ayobami Oshin
Biomedical Engineering Theses & Dissertations
Nanosecond pulsed electric field (nsPEF) employs nanosecond-duration, high voltage pulses to induce oxidative stress, leading to temporary or permanent damage to cells or tissue (also known as reversible and irreversible electroporation), and has been considered a promising approach for cancer therapy. In parallel to this, nanosecond pulsed atmospheric pressure plasma jets (ns-APPJs) have also shown to be effective in inactivating cancer cells or increasing sensitivity of cells to electric fields. ns-APPJs are known to generate reactive chemical agents, including reactive oxygen and nitrogen species (RONS) which induces oxidative stress resulting in cell proliferation, apoptosis, and necrosis. In this dissertation, a …
Low Dissolved Oxygen Operation Enhances Biological Phosphorus Removal In Wastewater Treatment, Haley Morgan
Low Dissolved Oxygen Operation Enhances Biological Phosphorus Removal In Wastewater Treatment, Haley Morgan
Civil & Environmental Engineering Theses & Dissertations
Biological phosphorus (bio-P) removal occurs when bacteria are exposed to anaerobic/aerobic conditions that stimulate polyphosphate-accumulating organisms (PAOs) to uptake phosphorus. In most treatment plants, dissolved oxygen (DO) concentrations in the aeration tanks are commonly at or above 2.0 mg O2/L, which is often referred to as the most optimal aeration condition for bio-P. If bio-P can happen efficiently at lower DO (< 0.3 mg O2/L), then the energy demand of aeration can be decreased appreciably. This research was conducted in a biological nutrient removal (BNR) pilot plant to assess the performance of bio-P at progressively lower DO operation. This …
Nonparametric Finite Mixture Of Ising Graphical Models, Manal Hamadi Alloqmani
Nonparametric Finite Mixture Of Ising Graphical Models, Manal Hamadi Alloqmani
Dissertations
Statistical applications in fields such as bioinformatics, genomics, speech processing, image processing, and communications often involve large-scale models in which thousands or millions of random variables are linked in complex ways. Graphical models provide a general methodology for approaching these problems, and indeed many of the models developed by researchers in these applied fields are instances of the general graphical model formalism. This formalism gives a nice framework for capturing complex dependencies among the random variables and building a large-scale model for high-dimensional data. Recently, high-dimensional data are more assumed to come from one population and follow a parametric or …
Resilient Learning For Anomaly Detection In Smart Living Systems, Sahar Abedzadeh
Resilient Learning For Anomaly Detection In Smart Living Systems, Sahar Abedzadeh
Dissertations
Cyber-Physical Systems (CPS) rely on anomaly-based detection methods to ensure the integrity and security of critical infrastructures such as smart grids, smart water metering systems, and advanced metering infrastructures (AMI). Anomaly detection methods are commonly used to identify deviations from normal system behavior by establishing learned profiles and thresholdbased distinctions between benign and anomalous events. However, conventional frameworks often fail to account for adversarial data poisoning attacks, unlabeled unsafe events, and environmental noise—factors that distort training data, degrade detection accuracy, and increase false alarms. This dissertation proposes a resilient learning framework that mitigates these biases by integrating quantile regression, M-estimation …
Utilizing Drainage Induced Pfas Enrichment In Cascade Generated Foam For Efficient Removal At Wastewater Treatment Plants, Ethan Samuel Coffin
Utilizing Drainage Induced Pfas Enrichment In Cascade Generated Foam For Efficient Removal At Wastewater Treatment Plants, Ethan Samuel Coffin
Dissertations
Observed trends in municipal solid waste landfills reveal a distinct disparity between per- and polyfluorinated alkyl substances (PFAS) composition entering in waste, mostly as diPAP and FTOH, and leaching out as FTCA and PFCA. These patterns are elucidated by compiling PFAS compositions in paper, textiles, and carpet, with known precursor transformations that generate FTCA and PFCA in leachate. Landfill leachate is commonly discharged to wastewater treatment plants (WWTP), which are ineffective at degrading PFAS and only serve as key conduits and discharge points for PFAS to the environment. Foam fractionation has been identified as a promising technology for concentrating and …
Valorization Of Industrial Wastes To Low-Carbon Minerals, Nandan Nag
Valorization Of Industrial Wastes To Low-Carbon Minerals, Nandan Nag
Civil & Environmental Engineering Theses & Dissertations
This study concentrates on developing methods for utilizing industrial waste materials such as red mud and phosphate slag. In this study, an electrochemical process was developed for recovering iron metal powder from red mud. Red mud is a waste material from aluminum refineries. Although red mud can be utilized as a construction and building material, its use is becoming controversial due to its high metal content, which contributes to its toxicity. However, the high iron oxide content in red mud makes it a good feedstock for producing iron. In this project, we employed an electrochemical technique for extracting iron powder …
Expanding The Lexa Bacterial Two-Hybrid System For Human Protein-Protein Interaction Screening: Application To Tumor Suppressor Par-4, Rebecca M. Richardson
Expanding The Lexa Bacterial Two-Hybrid System For Human Protein-Protein Interaction Screening: Application To Tumor Suppressor Par-4, Rebecca M. Richardson
Chemistry & Biochemistry Theses & Dissertations
Protein-protein interactions (PPI) are fundamental to cellular function, influencing processes such as signaling, structural organization, and disease progression. Disruptions in PPIs are implicated in cancer and neurodegenerative diseases, highlighting their importance as therapeutic targets. However, traditional methods for studying human PPIs, such as yeast two-hybrid and co-immunoprecipitation mass spectrometry, are costly and technically demanding. This dissertation explores the LexA-based bacterial two-hybrid (LexA-B2H) system as a cost effective and accessible alternative for investigating human PPIs.
This study focuses on the interaction between glucose-regulated protein 78 (GRP78) and cleaved prostate apoptosis response-4 (cl-Par-4), a tumor suppressor protein involved in apoptosis and neurodegenerative …