Digital Commons Network^™

Sorption And Transport Of Pharmaceutical Chemicals In Organic- And Mineral-Rich Soils, Vijay M. Vulava, Virginia Murphey, Samantha Kuzma, Jeff Schwindaman, Bradley Sion, Wendy Cory

S.C. Water Resources Conference

2012 S.C. Water Resources Conference - Exploring Opportunities for Collaborative Water Research, Policy and Management

Examining The Impact Of Pau Jacaré (Piptadenia Gonoacantha) Growth On Soil Fertility In The Brazilian Atlantic Rainforest, Aleksandra Ponomareva

Environmental Analysis Program Mellon Student Summer Research Reports

The Atlantic Rainforest of Brazil is an area of astounding diversity in both flora and fauna, taking its place among the top five “biodiversity hotspots” in the world. However, in the past 40 or 50 years the area has been increasingly threatened by the presence of humans – approximately 93% of the Rainforest has disappeared as a result of exploitation (Turner 2004). Unsustainable farming practices, as well as logging, cattle ranching and mining activities have caused soil infertility, water depletion, erosion and destruction of ecosystems. This project examines the effects of the Pau Jacaré, Piptadenia gonoacantha, tree on soil health …

2011-2012 Hccp Year-End Summary, Kristen S. Cloutier

Year-end Summaries

No abstract provided.

Traditional Wild Protein Collection Techniques Of The Naso People: On The Verge Of Change, Caldwell Rohrbach

Independent Study Project (ISP) Collection

I studied the traditional wild protein collection techniques of the Naso people in the Syellick community Naso Territory, Bocas del Toro for 15 days during the end of November. The goal of this research was to record the various hunting techniques used by the Naso and determine whether those practices will disappear after the passing of this generation. I used informal and unstructured interviews as well as participant, objective, and passive observation (Master of Business Administration).

The Naso people complete at least one form of wild protein collection every day. The given technique is directly related to the weather on …

Assessment Of Genetic Connectivity And Potential Management Plans For The Coral Reef Ecosystems Of Guna Yala, Panama, Molly Mcentee

Independent Study Project (ISP) Collection

Genetic connectivity between three populations of the common reef sea urchin, Echinometra lucunter located in fringing reefs off the islands of Ukuptupu, Wichupwala and Porvenir in western Guna Yala was analyzed to give insight into population distribution and the most effective conservation management plans for the area. The reefs of Guna Yala are facing many threats on a global or regional scale including global warming, ocean acidification, sea level rise, disease and severe storms. On a local level, increasing stress is being placed on these reef ecosystems from human activity including coral mining, overfishing, and pollution. In order to prevent …

Characterization Of Biofilm Formation By Borrelia Burgdorferi In Vitro, Eva Sapi, Scott L. Bastian, Cedric L. Mpoy, Shernea Scott, Amy Rattelle, Namrata Pabbati, Akhila Poruri, Divya Burugu, Priyanka A. Theophilus, Truc V. Pham, Akshita Datar, Navroop K. Dhaliwal, Alan Macdonald, Michael J. Rossi, Saion K. Sinha, David F. Luecke

Biology and Environmental Science Faculty Publications

Borrelia burgdorferi, the causative agent of Lyme disease, has long been known to be capable of forming aggregates and colonies. It was recently demonstrated that Borrelia burgdorferi aggregate formation dramatically changes the in vitro response to hostile environments by this pathogen. In this study, we investigated the hypothesis that these aggregates are indeed biofilms, structures whose resistance to unfavorable conditions are well documented. We studied Borrelia burgdorferi for several known hallmark features of biofilm, including structural rearrangements in the aggregates, variations in development on various substrate matrices and secretion of a protective extracellular polymeric substance (EPS) matrix using several modes …

A Novel Microfluidic Enrichment Technique For Carbonylated Proteins, Bryant C. Hollins

Doctoral Dissertations

Proteins are the building blocks of cells in living organisms, and are composed of amino acids. The expression of proteins is regulated by the processes of transcription and translation. Proteins undergo post-translational modifications in order to dictate their role physiologically within a cell.

Not all post-translational modifications are beneficial for the protein or the cell. One type of post-translational modification, called carbonylation, irreversibly places a carbonyl group onto an amino acid residue, most commonly proline, lysine, arginine, and threonine. This modification can have severe consequences physiologically, including loss of solubility, loss of function, and protein aggregation.

Carbonylated proteins have commonly …

Purification And Analysis Of Folate-Dye Conjugate, Sravya Mothe

All Capstone Projects

Folate receptor has achieved great importance for use in targeted drug delivery and in vitro and in vivo diagnostic assays. Folate receptor (FR) is over expressed on several cancer cell types and activated macrophages, but exists in relatively low abundance on the surface of non-cancerous tissue and non-inflammatory cells. This unique attribute is the hallmark of folate – targeted methodologies allowing the diagnostic agent and/or drug to be ferried to the specific disease sites while preventing “off target” effects in normal tissues. Although several folate-based dyes have been reported, simple and detailed synthetic and purification methods for these conjugates have …

Chemical Proteomics-Based Analysis Of Off-Target Binding Profiles For Rosiglitazone And Pioglitazone: Clues For Assessing Potential For Cardiotoxicity, Brian R. Hoffmann, Mohamed F. El-Mansy, Daniel S. Sem, Andrew S. Greene

Chemistry Faculty Research and Publications

Drugs exert desired and undesired effects based on their binding interactions with protein target(s) and off-target(s), providing evidence for drug efficacy and toxicity. Pioglitazone and rosiglitazone possess a common functional core, glitazone, which is considered a privileged scaffold upon which to build a drug selective for a given target—in this case, PPARγ. Herein, we report a retrospective analysis of two variants of the glitazone scaffold, pioglitazone and rosiglitazone, in an effort to identify off-target binding events in the rat heart to explain recently reported cardiovascular risk associated with these drugs. Our results suggest that glitazone has affinity for dehydrogenases, consistent …

Edible Marijuana: A New Frontier In The Culinary World, Ariella H. Wolkowicz

Honors Theses - Providence Campus

Cannabis, commonly known as marijuana, has a rich history as a source of fiber, food and medicine (Li 437). Since 1785, physicians and scientists alike have worked to discover the active chemical components and medical effectiveness of this plant (Touw 2; Aldrich). Despite its complicated legal history, marijuana has retained a place culturally and, in some countries, scientifically as an effective medical agent. As a medically edible ingredient, cannabis has also been more recently heralded as a new, even cutting edge flavor, opening a new frontier to the culinary community.

After the isolation of the main active ingredient in cannabis, …

Genetically Modified Organisms As A Potential Solution For Decreasing Hunger In Developing Nations: An Ethical Paradox, Rachel Sherman

Independent Study Project (ISP) Collection

I first became aware of and interested in genetically modified organisms (GMOs) in the “Politics of Food in America” course I took spring semester of 2012, my junior year at the University of Texas at Austin. I was intrigued by the fact that such a concept was so unfamiliar to the public, yet genetically engineered products were in our everyday foods. Though I received a basic introduction to GMOs through my course, I was curious to learn more about the regulatory practices of GM crops and the political relationships sustained by GM firms and government agencies in the United States. …

Enhancement Of Vitamin D Metabolites In The Eye Following Vitamin D3 Supplementation And Uv-B Irradiation, Yanping Lin, John L. Ubels, Mark P. Schotanus, Zhaohong Yin

University Faculty Publications and Creative Works

This study was designed to measure vitamin D metabolites in the aqueous and vitreous humor and in tear fluid, and to determine if dietary vitamin D3 supplementation affects these levels. We also determined if the corneal epithelium can synthesize vitamin D following UV-B exposure. Methods: Rabbits were fed a control or vitamin D3 supplemented diet. Pilocarpine- stimulated tear fluid was collected and aqueous and vitreous humor were drawn from enucleated eyes. Plasma vitamin D was also measured. To test for epithelial vitamin D synthesis, a human corneal limbal epithelial cell line was irradiated with two doses of UV-B (10 and …

Slides: Session 3: Decision-Making And The Energy Poor, Andrew Yager

2012 Energy Justice Conference and Technology Exposition (September 17-18)

Presenter: Andrew Yager, United Nations Department of Economic and Social Affairs, Division for Sustainable Development

33 slides

Effects Of Genetic Variants Previously Associated With Fasting Glucose And Insulin In The Diabetes Prevention Program, Jose C. Florez, Kathleen A. Jablonski, Jarred B. Mcateer, Paul W. Franks, Clinton C. Mason, Kieren J. Mather, Edward Horton, Ronald Goldberg, Dana Dabelea, Steven E. Kahn, Richard F. Arakaki, Alan R. Shuldiner, William C. Knowler

Epidemiology Faculty Publications

Common genetic variants have been recently associated with fasting glucose and insulin levels in white populations. Whether these associations replicate in pre-diabetes is not known. We extended these findings to the Diabetes Prevention Program, a clinical trial in which participants at high risk for diabetes were randomized to placebo, lifestyle modification or metformin for diabetes prevention. We genotyped previously reported polymorphisms (or their proxies) in/near G6PC2, MTNR1B, GCK, DGKB, GCKR, ADCY5, MADD, CRY2, ADRA2A,FADS1, PROX1, SLC2A2, GLIS3, C2CD4B, IGF1, and IRS1 in 3,548 Diabetes …

An Economic Analysis Of Wine Grape Production In The State Of Connecticut, Jeremy L. Jelliffe

Master's Theses

The Connecticut Wine and Vineyard industry has grown at a steady 3.9% per year over the past decade (ATTTB, 2009). Economic models estimate that the wineries sub-sector contributes $38 million dollars to the state economy and direct employment of 106 residents (Lopez et al., 2010). Programs to support and foster further growth of the industry and CT farm vineyard culture include the Department of Agriculture’s CT Wine Trail and the annual CT Wine festival (DOAG, 2010). Farmland preservation groups also support vineyard development since grape growing tends to secure tracts of farmland for long periods of time.

Investment analysis for …

The Local Food Movement: Definitions, Benefits, And Resources, Roslynn Brain

Environment and Society Faculty Publications

What does it mean to eat locally? Although perceptions of what makes up “local food” differ by region (due in large part to varying climates, soil types, and populations), most researchers accept that eating locally means minimizing the distance between production and consumption, especially in relation to the modern mainstream food system (Peters et al., 2008). According to the 2008 Farm Act, a product can be marketed as locally or regionally produced if its end-point purchase is within 400 miles from its origin, or within state boundaries (Martinez et al., 2010). Most retailers, however, consider local to be on a …

Measuring Infertility In Populations: Constructing A Standard Definition For Use With Demographic And Reproductive Health Surveys, Maya N. Mascarenhas, Hoiwan Cheung, Colin D. Mathers, Gretchen A. Stevens

Dartmouth Scholarship

Background: Infertility is a significant disability, yet there are no reliable estimates of its global prevalence. Studies on infertility prevalence define the condition inconsistently, rendering the comparison of studies or quantitative summaries of the literature difficult. This study analyzed key components of infertility to develop a definition that can be consistently applied to globally available household survey data.

Methods: We proposed a standard definition of infertility and used it to generate prevalence estimates using 53 Demographic and Health Surveys (DHS). The analysis was restricted to the subset of DHS that contained detailed fertility information collected through the reproductive health calendar. …

Comparative Transcriptome Analysis Of Two Olive Cultivars In Response To Nacl-Stress, Christos Bazakos, Maria E. Manioudaki, Ioannis Therios, Demetrios Voyiatzis, Dimitris Kafetzopoulos, Tala Awada, Panagiotis Kalaitzis

School of Natural Resources: Faculty Publications

Background: Olive (Olea europaea L.) cultivation is rapidly expanding and low quality saline water is often used for irrigation. The molecular basis of salt tolerance in olive, though, has not yet been investigated at a system level. In this study a comparative transcriptomics approach was used as a tool to unravel gene regulatory networks underlying salinity response in olive trees by simulating as much as possible olive growing conditions in the field. Specifically, we investigated the genotype-dependent differences in the transcriptome response of two olive cultivars, a salt-tolerant and a salt-sensitive one.

Methodology/Principal Findings: A 135-day long …

Genetic Modulation Of Lipid Profiles Following Lifestyle Modification Or Metformin Treatment: The Diabetes Prevention Program, Toni I. Pollin, Tamara Isakova, Kathleen A. Jablonski, Paul I.W. De Bakker, Andrew Taylor, Jarred B. Mcateer, Qing Pan, Edward Horton, Linda M. Delahanty, David Altshuler, Alan R. Shuldiner, Ronald Goldberg, Jose C. Florez, George A. Bray

Epidemiology Faculty Publications

Weight-loss interventions generally improve lipid profiles and reduce cardiovascular disease risk, but effects are variable and may depend on genetic factors. We performed a genetic association analysis of data from 2,993 participants in the Diabetes Prevention Program to test the hypotheses that a genetic risk score (GRS) based on deleterious alleles at 32 lipid-associated single-nucleotide polymorphisms modifies the effects of lifestyle and/or metformin interventions on lipid levels and nuclear magnetic resonance (NMR) lipoprotein subfraction size and number. Twenty-three loci previously associated with fasting LDL-C, HDL-C, or triglycerides replicated (P = 0.04–1×10−17). Except for total HDL particles (r = −0.03, …

Trace Element Soil Contamination At Urban Community Gardens In Washington, Dc, Adam J. Long

Environmental Analysis Program Mellon Student Summer Research Reports

In recent years, urban gardening has become a popular form of environmental, food, and social justice. Urban community gardens such as those in Washington, DC can reduce the environmental footprint of food production, provide access to healthy produce in “food deserts,” and provide other social, educational, and even financial benefits. However, the rising popularity of urban gardening has put many people in close contact with urban soils, which are likely to contain various contaminants due to concentrated human activity over extended periods of time. This study investigates heavy metal soil contaminants found in community gardens located in Washington, DC. 45 …

Locust Dynamics: Behavioral Phase Change And Swarming, Chad M. Topaz, Maria R. D'Orsogna, Leah Edelstein-Keshet, Andrew J. Bernoff

All HMC Faculty Publications and Research

Locusts exhibit two interconvertible behavioral phases, solitarious and gregarious. While solitarious individuals are repelled from other locusts, gregarious insects are attracted to conspecifics and can form large aggregations such as marching hopper bands. Numerous biological experiments at the individual level have shown how crowding biases conversion towards the gregarious form. To understand the formation of marching locust hopper bands, we study phase change at the collective level, and in a quantitative framework. Specifically, we construct a partial integrodifferential equation model incorporating the interplay between phase change and spatial movement at the individual level in order to predict the dynamics of …

Pastoral Lease Assessment Using Geospatial Analysis, T P. Robinson, P E. Novelly, R Corner, Philip Thomas, A Russell-Browm

Resource management technical reports

Seven remote sensing based Vegetation Indices (NDSVI, STVI-3, NDVI, Green + Red, Red, Land Monitor and STVI-1) were tested for their potential to discriminate between “poor” and “good” range condition assessments in Western Australia’s pastoral rangelands. Indices were computed using the Australian Greenhouse Office National Carbon Accounting System (AGO NCAS) repository of calibrated Landsat TM/ETM+ mosaics. Discrimination potential was assessed at two levels of stratification – station level (identical to the stratification used to collect the traverse data), and functional group level (an aggregation of similar land systems);

Fall In C-Peptide During First 2 Years From Diagnosis: Evidence Of At Least Two Distinct Phases From Composite Type 1 Diabetes Trialnet Data., Carla J. Greenbaum, Craig A. Beam, David Boulware, Stephen E. Gitelman, Peter A. Gottlieb, Kevan C. Herold, John M. Lachin, Paula L. Mcgee, Jerry P. Palmer, Mark D. Pescovitz, Heidi Krause-Steinrauf, Jay S. Skyler, Jay M. Sosenko

Epidemiology Faculty Publications

Interpretation of clinical trials to alter the decline in β-cell function after diagnosis of type 1 diabetes depends on a robust understanding of the natural history of disease. Combining data from the Type 1 Diabetes TrialNet studies, we describe the natural history of β-cell function from shortly after diagnosis through 2 years post study randomization, assess the degree of variability between patients, and investigate factors that may be related to C-peptide preservation or loss. We found that 93% of individuals have detectable C-peptide 2 years from diagnosis. In 11% of subjects, there was no significant fall from baseline by 2 …

The Plant Based Diet: Increasing Overall Health Through Simple Diet, Tyler Sorensen

Department of Environmental Studies: Undergraduate Student Theses

No abstract provided.

Reactions Of Methyl Perfluoroalkyl Ethers With Isopropyl Alcohol: Experimental And Theoretical Studies, Howard Knachel, Vladimir Benin, Chadwick Barklay, Janine C. Birkbeck, Billy D. Faubion, William E. Moddeman

Chemistry Faculty Publications

The reaction of an isomeric mixture of the methyl perfluoroalkyl ether, C4F9OCH3 (Novec-7100), in the presence of isopropyl alcohol (IPA) and/or water has been studied by measuring the rate of product formation using an ion-selective electrode (ISE) for fluoride ion, Karl Fisher coulometric titrations for water, and 1H and 19F NMR spectroscopy for product identification and rate studies. The results showed the methyl perfluoroalkyl ether to be very stable with products forming at the rate of ∼1 ppm per year at a laboratory temperature of 20 °C. Measurements over the temperature range of 6° to 100 °C were made on …

The Consumption Of Dha During Embryogenesis As An Indicative Of The Need To Supply Dha During Early Larval Development: A Review, Joana Figueiredo, Junda Lin, Justin Anto, Luis Narciso

Marine & Environmental Sciences Faculty Articles

The establishment of an adequate larval diet for crustacean and fish often involves a series of time-consuming and expensive trial and errors. Despite being nutritionally poor, rotifers and Artemia are the most commonly used preys in larviculture. Whether (and to what extent) the prey needs to be enriched with essential fatty acids differs from species to species. We hypothesized that the DHA content of a newly spawned eggs and its consumption through embryogenesis can be a good indicator of the need to enrich the prey with DHA. In order to assess this hypothesis, we performed a search in the scientific …

Genetic Susceptibility To Type 2 Diabetes: A Global Meta-Analysis Studying The Genetic Differences In Tunisian Populations, Rym Berhouma, S. Kouidhi, M. Ammar, H. Abid, T. Baroudi, H. Ennafaa, A. Benammar-Elgaaied

Human Biology Open Access Pre-Prints

The present study is the first meta-analysis to evaluate type 2 diabetes (T2D) - associated polymorphisms in cohorts originated from several Tunisian regions. In fact, we evaluated the effect of seven polymorphisms in the following genes; PPARg ( Pro12Ala), TNFα (-308A/G), ENPP1(K121Q), TCF7L2(rs7903146 C/T), MTHFR( C677T), ACE(I/D), CAPN10(3R/2R) on T2D risk, through a meta-analysis combining data of previous studies performed on Tunisian populations originating from the north, centre or south of the country. R statistics version 2.12.1 software was used to estimate the heterogeneity between studies. Pooled ORs were computed by the fixed-effects method of Mantel-Haenszel if no heterogeneity between …

What Factors Influence Consumers' Willingness To Purchase And Consume Carbon-Labeled Rice: A Comparative Study Between Arkansas And Belgium, John A. Kelley

Graduate Theses and Dissertations

Climate change is an issue of growing importance around the globe. As global population increases so does attention focused on human contributions to climate change. One major way humans contribute to climate change is through our diets. Agricultural production is a major source of global greenhouse emissions. It has been estimated that food production is responsible for a large majority of the methane and nitrous oxide which is emitted into our atmosphere each year. Carbon footprint labeling has been developed to give consumers a way to lower their footprint and help lower emissions of greenhouse gasses. At least one retailer, …

Selenium Removal In Nutrient-Amended Pilot-Scale Constructed Wetland Treatment Systems, Peter Van Heest

All Theses

ABSTRACT
In a year-long study, pilot-scale constructed wetland treatment systems (CWTSs) targeted removal of selenium (Se) from a simulated impaired water through dissimilatory Se reduction. Effects of temperature and precipitation on the treatment of Se in the CWTSs were investigated. The experimental design consisted of CWTSs amended with a fermented yeast product (AquaSmartTM, Diamond V¨, Cedar Rapids, IA), which served as a nutrient source for Se-reducing bacteria, at mass loadings of 1.75, 3.5, 5.25, 7, and 8.75 mg/min. The 5 µg/L treatment goal for CWTS outflow concentration was measured in 69 of 378 samples taken from all CWTSs. Of the …

Novel Data As A Source For Assessing Breeding Phenology Of White-Tailed Deer (Odocoileus Virginianus) In South Carolina, David Stone

All Theses

White-tailed deer (Odocoileus virginianus) breeding phenology varies temporally throughout the species' range and is critical to population management and hunter enjoyment. The South Carolina Department of Natural Resources (SCDNR) demographic and reproductive data, collected primarily from 1970-1992, suggest that 81.86% of conceptions in the Coastal Plain occur between 6 October and 16 November. However, current data regarding conception dates are lacking for a variety of reasons including the current absence of check stations and the use of hunter surveys as the primary means of data collection. To more closely examine recent characteristics of the white-tailed deer breeding season phenology, we …