Digital Commons Network^™

Some Effects Of Nutrient And Flooding Stress Manipulation On Coastal Louisiana Wetland Vegetation, James Stephen Ialeggio

LSU Master's Theses

Freshwater diversions are a relatively recently utilized tool in wetland loss mitigation that stimulate an organic accretion response in marsh vegetation, which is based in root production and thus belowground biomass. The effectiveness of freshwater diversions in slowing marsh loss probably varies across a gradient of the factors they supply: decreased salinity, increased nutrient concentrations, and increased inundation. Flooding stress is commonly thought to be the overriding factor limiting wetland vegetation growth, however its influence may vary across salinity and nutrients. Therefore, plugs of Spartina patens were planted in four “marsh organs” consisting each of 36 sediment-filled PVC pipes of …

Matrix-Assisted Laser Desorption Ionization Mass Spectrometry For Identificaiton Of Shrimp, Venkatesulu Salla

LSU Master's Theses

Matrix-assisted laser desorption ionization (MALDI) time of flight mass spectrometry was used to identify shrimp at the species level using commercial mass spectral fingerprint matching software (Bruker Biotyper). In the first step, a mass spectrum reference database was constructed from the analysis of six shrimp species that are commercially important in the United States: L. setiferus, F. azticus, S. brevirostris, P. robustus, P. dispar and P. platyceros. In the second step, the reference database was tested using 74 unknown shrimp samples from these six species. Correct identification was achieved for 72 of the 74 samples (97 percent): 72 samples were …

Exploring Local Food System Practices And Perceptions: Insights From Florida's Snap-Authorized Farmers' Markets, Leslie Babiak

USF Tampa Graduate Theses and Dissertations

Despite heightened interest in creating local food systems that enhance health of ecologies, economies, and all members of communities, the public space of farmers' markets is far less than inherently equitable. This is particularly concerning given America's unprecedented crisis of food hardship and related disease, which disproportionately affects lower income populations. This research addresses the social justice implications of SNAP (food stamp) operations for locally oriented food systems. Pioneering practices of three of Florida's SNAP-authorized farmers' markets, and the attitudes and behaviors of one-hundred-seventy-six market patrons, were explored through customer surveys, market manager interviews, and environmental assessments. Qualitative and quantitative …

Virtual Screening For Inhibitors Of Anti-Apoptotic Proteins: Dck, Bcl-Xl, Mcl-1, Mdmx, And Mdm2, Courtney Jerome Du Boulay

USF Tampa Graduate Theses and Dissertations

←Within this dissertation the topic of virtual screening is discussed with regard to three different cancer targets and also a brief introduction of the tools used in virtual screening. In Chapter 1, the reader will be introduced to virtual screening and the programs that are used in virtual screening. In Chapter 2, the first of three projects are discussed. This project consists of the work that was done to find inhibitors of the P53 binding domain of MDMX. In this project the mobility of residues within the binding site of MDMX are discussed and the ways in which we attempted …

The Water Culture Beliefs Of Embera Communities And Maternal And Child Health In The Republic Of Panama, Ilenia Anneth Forero

USF Tampa Graduate Theses and Dissertations

Water has cultural and spiritual values to indigenous people. These beliefs expose them to unsafe water sources and make them vulnerable to waterborne diseases. This background is not taken into account when countries write their water legislations, therefor imposing a management of water not readily accepted by them. The Embera group is one of the indigenous groups from the Republic of Panama, who have strong water beliefs. They live along the shore of rivers in houses built on high stilts away from urban areas. The purpose of this cross-sectional community based study is to describe through a survey the relation …

Metabolism In Corals From Antarctica, The Deep-Sea, And The Shallow Subtropics: Contrasts In Temperature, Depth, And Light, Lara Henry

USF Tampa Graduate Theses and Dissertations

Coral habitats span the range from tropical to polar, extremely shallow to thousands of meters deep. The differences in light and temperature experienced in these varied habitats likely affect the metabolic rates of the corals residing there. The metabolism of three coral species from different habitats have been examined to elucidate the effects of these environmental parameters on metabolism, an under-studied aspect of coral biology. For all three species, measurements of oxygen uptake, ammonium excretion, and activity of the enzymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), and citrate synthase (CS) were used to characterize their metabolism. Off Florida's Gulf coast, …

Monitoring The Survival Of Hatchery-Produced Spat And Larvae On Louisiana Public Oyster Reefs, Erin R. Leonhardt

LSU Master's Theses

Alternative or supplemental management activities may be necessary to restore and enhance oyster production on Louisiana public oyster reefs. The production of wild oysters is variable due to anthropogenic and environmental factors that affect recruitment, growth and survival. The availability and structure of cultch material for larval recruitment and survival is particularly important to maintain oyster production. Beginning in 2011, the Sea Grant Oyster Hatchery on Grand Isle, LA and the Louisiana Department of Wildlife and Fisheries (LDWF) collaborated to test the survival of hatchery-produced spat and hatchery-produced larvae deployed on public oyster grounds and cultch plant sites. In 2011, …

Wetting And Drying Cycles And The Fungal Communities On Leaf Litter In Streams, Kele Qwinn Thrailkill

Masters Theses

"Leaf litter is a major source of energy for streams in deciduous forests. Fungi play a critical role by converting the leaves into nutritional material for the rest of the food web. The breakdown of leaf litter and associated biota, including invertebrates and fungi, have been proposed as measures of stream health in systems affected by anthropogenic activity. Rates of leaf breakdown can be depressed in streams affected by acidity, metals, organic contaminants, and other stressors. Climate change may lead to alterations in stream hydrology such that streams experience more frequent floods as well as drying episodes. Fungal communities can …

Effects Of Temperature On The Physicochemical Properties Of Traditionally Processed Vegetable Oils And Their Blends, Fn Ngassapa, Ss Nyandoroa, Tr Mwaisaka

Tanzania Journal of Science

The quality and stability of traditionally processed palm, sunflower and sesame oils and their blends as affected by temperature were evaluated by analyzing their physicochemical properties which included acid, saponification, peroxide, iodine values and refractive indices.. The initial quality of traditionally processed oils studied was found to be within the specifications of TZS and Codex requirements. The results further indicate that the quality of both unblended and blended traditionally processed vegetable oils deteriorate significantly at elevated temperatures (p < 0.05). However, the blends showed relatively better quality and stability than the unblended ones whereby the formulations of palm oil (PO) and sesame oil (SSO) appeared to be more stable than those of PO and sunflower oil (SO). This work suggests that blending of traditionally processed PO, SO and SSO improves the quality and stability of these oils.

Assessing The Human Impact Deer Frequency And Activity In The Depauw University Nature Park, Amanda Faulkenberg

Student Research

Introduction
White-tailed deer are abundant in the DePauw Nature Park. There are many deer trails located in forested areas in the park. The goal of our research was to assess the relationship between the amount of deer trails and the abundance of deer.

Hypotheses
We hypothesized that more deer would be photographed by cameras located in areas with the most deer trails.

Methods
We conducted our research in two forested areas, Quarry Hillside and Quarry South, in the DePauw Nature Park. We randomly selected five plots in each area. We mapped each deer trail and measured the length, width, and …

Seasonal Dimorphism In The Horny Bills Of Sparrows, Russell Greenberg, Matthew Etterson, Raymond M. Danner

United States Environmental Protection Agency: Publications

Bill size is often viewed as a species-specific adaptation for feeding, but it sometimes varies between sexes, suggesting that sexual selection or intersexual competition may also be important. Hypotheses to explain sexual dimorphism in avian bill size include divergence in feeding niche or thermoregulatory demands, intrasexual selection based on increased competition among males, or female preference. Birds also show seasonal changes in bill size due to shifts in the balance between growth rate and wear, which may be due to diet or endogenous rhythms in growth. Insight into the function of dimorphism can be gained using the novel approach of …

Walmart's Sustainability Journey: Defining Sustainable Products (B), David G. Hyatt, Andrew Spicer

Wal-Mart Sustainability Case Project

In “Defining Sustainable Products (A),” we examine Walmart’s search for a systemic solution to define a sustainable product precisely. In this case, Matt Kistler, who succeeded Andy Ruben as the company sustainability leader in 2007, knows that the company is lagging behind on its espoused goal of selling sustainable products. The case asks students step into Kistler’s shoes as he leads Walmart’s sustainability office through a systematic review of the different models and frameworks available for measuring the sustainability of products presently in use. Eventually, the students face a decision about what Walmart should do to make systemic progress with …

Walmart's Sustainability Journey: Defining Sustainable Products (A), David G. Hyatt, Andrew Spicer

Wal-Mart Sustainability Case Project

In “Defining Sustainable Products (A),” we examine Walmart’s search for a systemic solution to define a sustainable product precisely. In this case, Matt Kistler, who succeeded Andy Ruben as the company sustainability leader in 2007, knows that the company is lagging behind on its espoused goal of selling sustainable products. The case asks students step into Kistler’s shoes as he leads Walmart’s sustainability office through a systematic review of the different models and frameworks available for measuring the sustainability of products presently in use. Eventually, the students face a decision about what Walmart should do to make systemic progress with …

Microfluidic Experiments Reveal That Antifreeze Proteins Bound To Ice Crystals Suffice To Prevent Their Growth, Yeliz Celik, Ran Drori, Natalya Pertaya-Braun, Aysun Altan, Tyler Barton, Maya Bar-Dolev, Alex Groisman, Peter L. Davies, Ido Braslavsky

Physics Faculty Research

Antifreeze proteins (AFPs) are a subset of ice-binding proteins that control ice crystal growth. They have potential for the cryopreservation of cells, tissues, and organs, as well as for production and storage of food and protection of crops from frost. However, the detailed mechanism of action of AFPs is still unclear. Specifically, there is controversy regarding reversibility of binding of AFPs to crystal surfaces. The experimentally observed dependence of activity of AFPs on their concentration in solution appears to indicate that the binding is reversible. Here, by a series of experiments in temperature-controlled microfluidic devices, where the medium surrounding ice …

Progression Magazine, 2012-2013, Coastal Carolina University

Progression Magazine

Magazine of the College of Science at Coastal Carolina University.

Interactions Of Zoo Plankton And Phytoplankton With Cyanobacteria, Rebecca J. Alexander

School of Natural Resources: Dissertations, Theses, and Student Research

Cyanobacteria are a major concern in Nebraska reservoirs and are capable of producing toxins that can cause skin irritations and gastrointestinal problems, as well as affect the nervous system. It is important to determine the mechanisms that can cause cyanobacteria blooms due to the effect they can have on human health. The interaction of zooplankton and other phytoplankton groups with cyanobacteria is important because there is a biological component in surface waters that should be taken into consideration along with the physical and chemical parameters that have been noted to promote cyanobacteria. For example, zooplankton have the ability to alter …

Interactions Of Zooplankton And Phytoplankton With Cyanobacteria, Rebecca Alexander

School of Natural Resources: Dissertations, Theses, and Student Research

Cyanobacteria are a major concern in Nebraska reservoirs and are capable of producing toxins that can cause skin irritations and gastrointestinal problems, as well as affect the nervous system. It is important to determine the mechanisms that can cause cyanobacteria blooms due to the effect they can have on human health. The interaction of zooplankton and other phytoplankton groups with cyanobacteria is important because there is a biological component in surface waters that should be taken into consideration along with the physical and chemical parameters that have been noted to promote cyanobacteria. For example, zooplankton have the ability to alter …

Limited Sampling Estimates Of Epigallocatechin Gallate Exposures In Cirrhotic And Noncirrhotic Patients With Hepatitis C After Single Oral Doses Of Green Tea Extract., Dina Halegoua-De Marzio, Walter K. Kraft, Constantine Daskalakis, Xie Ying, Roy L Hawke, Victor J. Navarro

Division of Gastroenterology and Hepatology Faculty Papers

BACKGROUND: Epigallocatechin-3-gallate (EGCG) has antiangiogenic, antioxidant, and antifibrotic properties that may have therapeutic potential for the treatment of cirrhosis induced by hepatitis C virus (HCV). However, cirrhosis might affect EGCG disposition and augment its reported dose-dependent hepatotoxic potential.

OBJECTIVE: The safety, tolerability, and disposition of a single oral dose of EGCG in cirrhotic patients with HCV were examined in an exploratory fashion.

METHODS: Eleven patients with hepatitis C and detectable viremia were enrolled. Four had Child-Pugh (CP) class A cirrhosis, 4 had Child-Pugh class B cirrhosis, and 3 were noncirrhotic. After a single oral dose of green tea extract 400 …

In Vitro Assessment Of The Survivability Of Lactobacillus Casei Dn-114 001 And Lactobacillus Casei Shirota, Within Commercialised Food Matrices, In The Upper Gastrointestinal Tract, Rosie Wills

The Plymouth Student Scientist

The aim of this study was to determine the survivability of probiotics; Lactobacillus casei Shirota and Lactobacillus casei DN-114 001, within their respective commercialised food matrices YakultÃ’ and ActimelÃ’, during in vitro simulated gastrointestinal transit. Original and Light/Fat Free varieties of each brand were assessed to determine whether nutritional composition affected bacterial survival rate. Strains were exposed to 3 hours of simulated gastric transit at pH 2, using Hydrochloric Acid (32%) and Pepsin (3mg ml-1), followed by 2 hours of simulated duodenal transit, at pH 6.5, using Bile Salts (0.3% w/v) and Sodium Hydroxide (4M). Samples were serially diluted with …

A Preliminary Study Into The Use Of Canopy Invertebrates And Sampling Techniques In Relation To Forest Indicators In A Fragmented Scottish Woodland - Application And Management, Natalie Walsh

The Plymouth Student Scientist

Caledonian forests within the Scottish Highlands have experienced severe fragmentation and currently remain as small isolated habitats. Canopy invertebrates within these forests play an important role in ecosystem functioning and may be potentially used as forest indicators for the use in forest management. Four different low-cost invertebrate trapping methods were deployed into birch, oak and Scots pine trees, comparing family diversity and composition between tree and trap types. Data on canopy spread, tree height and d.b.h were also tested to see if they influenced family composition of invertebrates. Collected data found little difference in family diversity and composition between tree …

The Positive Effects Of Aquarium Visits On Children's Behaviour: A Behavioural Observation, Hazel Cocker

The Plymouth Student Scientist

To date research has found a positive effect of blue spaces on adults’ wellbeing. However, it remains to be seen whether these findings are also true for children. The present study aims to address this gap in the literature. The researchers observed children’s behaviour at the National Marine Aquarium across two weeks, including one where the Big Draw event was taking place. In line with restorative theories of natural environments (Kaplan, 1995, Ulrich, 1983), the current research found the exhibit had a calming effect on behaviour and increased levels of happiness across both weeks. During the Big Draw week children …

The Impact Of Creosote Bush (Larrea Tridentata) And Biological Soil Crust On Ca Distribution In Arid Soils Of The Mojave Desert, Brittany Myers

UNLV Theses, Dissertations, Professional Papers, and Capstones

Ca is an important nutrient that plays a role in membrane stability and cell repair in plant life. This study examines the impact of creosote bush (Larrea tridentata) and biological soil crust on calcium cycling and distribution in desert soils in order to explore the use of Ca as a biosignature. Samples of creosote bush, biological soil crust and eolian dust were taken at two field sites in the Mojave Desert. The first site is located in Eldorado Valley, NV, a soil formed on a young (800-1200 years) alluvial fan deposit; the second site is located on a late Holocene-aged …

Sample Preparation Techniques For The Improvement Of Analytical Measurements: Capillary-Channeled Polymer Fibers Employed In Protein Solid Phase Extraction Applications And Characterization Of Active Components In Botanical Dietary Supplements, Carolyn Burdette

All Dissertations

The task of sample preparation encompasses many challenges and demands for analytical techniques. Appropriate preparative steps can be the determining factor between successful measurements and failures. Too little preparation can lead to the inability to detect analytes due to low concentrations, insufficient extraction of analytes, and/or strong matrix effects; exhaustive preparation steps increases analysis times, the potential for analyte losses, and the difficulty to obtain consistent reproducible results. Discussed here are several examples of analytical techniques where sample preparation is critical and can be used to improve existing techniques. Capillary-channeled polymer (C-CP) fibers used for solid phase extraction (SPE) protein …

Long-Term Integrated Studies Show Complex And Surprising Effects Of Climate Change In The Northern Hardwood Forest, Peter M. Groffman, Lindsay E. Rustad, Pamela H. Templer, John L. Campbell, Lynn M. Christenson, Nina K. Lany, Anne M. Socci, Matthew A. Vadeboncoeur, Paul G. Schaberg, Geoffrey W. Wilson, Charles T. Driscoll, Timothy J. Fahey, Melany C. Fisk, Christine L. Goodale, Mark B. Green, Steven P. Hamburg, Chris E. Johnson, Mryon J. Mitchell, Jennifer L. Morse, Linda H. Pardo, Nicholas L. Rodenhouse

Environmental Science and Management Faculty Publications and Presentations

Evaluations of the local effects of global change are often confounded by the interactions of natural and anthropogenic factors that overshadow the effects of climate changes on ecosystems. Long-term watershed and natural elevation gradient studies at the Hubbard Brook Experimental Forest and in the surrounding region show surprising results demonstrating the effects of climate change on hydrologic variables (e.g., evapotranspiration, streamflow, soil moisture); the importance of changes in phenology on water, carbon, and nitrogen fluxes during critical seasonal transition periods; winter climate change effects on plant and animal community composition and ecosystem services; and the effects of anthropogenic disturbances and …

The Effects Of River Sediment, Endosulfan, And Moderate Hypoxia On Blue Crabs (Callinectes Sapidus) From The Tidal, Freshwater James River, Laura Williams

Theses and Dissertations

Juvenile male blue crabs move into the tidal, freshwater James River during warmer months to feed and grow by undergoing molting. In crustaceans, growth and molting are hormonally controlled. The physiological effects of a multiple-stressor environment are determined by comparing the blue crab’s oxygen uptake after exposure to pure sand, James River sediment, or endosulfan-spiked sand. The effect of multiple stressors on molting is measured by the activity level of N-acetyl-ß-glucosaminidase (NAG), an enzyme in epidermal tissue important to molting. The oxygen uptake was decreased by exposure to James River sediment but not for exposure to endosulfan for seven days. …

Obtaining Consumer Perceptions Of External Costs Of “Cheap” Food Products And Analyzing Quality Food Markets That Minimize External Costs In Bloomington-Normal, Illinois, Jennifer Long '13

Outstanding Senior Seminar Papers

Today, the United States’ food system is primarily a large industrial operation with smaller-scale community-based food systems. Although the industrial food system has benefitted society by increasing the amount of food available for every person, some of these products are “cheap” food products that generate external costs, such as poor health, potential wealth loss to farmers and environmental degradation. With over 1 billion individuals on earth undernourished and 15.8% of all U.S. households as of 2010 food insecure [Patel, 2012], this system has not completely solved food issues. Community-based food systems, on the other hand, minimize external costs …

Lonomics Atlas: A Tool To Explore Interconnected Ionomic, Genomic And Environmental Data, Eduard Dragut, Amgad Madkour, Mohamed Nabeel, Peter Baker, Mourad Ouzzani, David Salt

Cyber Center Publications

Ionomics Atlas facilitates access, analysis and interpretation of an existing large-scale heterogeneous dataset consisting of ionomic (elemental composition of an organism), ge- netic (heritable changes in the DNA of an organism) and ge- ographic information (geographic location, altitude, climate, soil properties, etc). Ionomics Atlas allows connections to be made between the genetic regulation of the ionome of plant populations and their landscape distribution, allowing scientists to investigate the role of natural ionomic variation in adaptation of populations to varied environmental conditions in the landscape. The goal of the Ionomics Atlas is twofold: (1) to allow both novice and expert users …

Electrochemical Detection Of Ammonia In Aqueous Solution Using Fluorescamine: A Comparison Of Fluorometric Versus Voltammetric Analysis, Panchompoo Janjira, G. Compton Richard

Journal of Electrochemistry

Fluorescamine is a non-fluorescent reagent widely used for the quantitative determination of primary amines by fluorescence spectroscopy as it reacts readily with primary amines to form a fluorescent product. In this work, a new sensitive voltammetric method for the detection of ammonia in aqueous solution by the reaction with fluorescamine has been developed. First, the electrochemical behaviour of fluorescamine in the absence and presence of ammonia was investigated in 0.1 mol L^-1 borate buffer solution (pH 9.0) by cyclic voltammetry using a glassy carbon (GC) electrode. As for fluorescamine itself, a well-defined irreversible oxidation peak could be observed at …

Spectral Cross Correlation As A Supervised Approach For The Analysis Of Complex Raman Datasets: The Case Of Nanoparticles In Biological Cells, Mark Keating, Franck Bonnier, Hugh Byrne

Articles

Spectral Cross-correlation is introduced as a methodology to identify the presence and subcellular distribution of nanoparticles in cells. Raman microscopy is employed to spectroscopically image biological cells previously exposed to polystyrene nanoparticles, as a model for the study of nano-bio interactions. The limitations of previously deployed strategies of K-means clustering analysis and principal component analysis are discussed and a novel methodology of Spectral Cross Correlation Analysis is introduced and compared with the performance of Classical Least Squares Analysis, in both unsupervised and supervised modes. The previous study demonstrated the feasibility of using Raman spectroscopy to map cells and identify polystyrene …

Total And Methyl Mercury In Surface Water And Sediment From Differing Ecoregions Of South Carolina, Jane L. Guentzel, David Graves, James Glover

S.C. Water Resources Conference

2012 S.C. Water Resources Conference - Exploring Opportunities for Collaborative Water Research, Policy and Management