Digital Commons Network^™

A Gene-Based Recessive Diplotype Exome Scan Discovers Fgf6, A Novel Hepcidin-Regulating Iron-Metabolism Gene, Shicheng Guo, Shuai Jiang, Narendranath Epperla, Yanyun Ma, Mehdi Maadooliat, Zhan Ye, Brent Olson, Minghua Wang, Terrie Kitchner, Jeffrey Joyce, Peng An, Fudi Wang, Robert Strenn, Joseph J. Mazza, Jennifer K. Meece, Wenyu Wu, Li Jin, Judith A. Smith, Jiucan Wang, Steven J. Schrodi

Mathematical and Statistical Science Faculty Research and Publications

Standard analyses applied to genome-wide association data are well designed to detect additive effects of moderate strength. However, the power for standard genome-wide association study (GWAS) analyses to identify effects from recessive diplotypes is not typically high. We proposed and conducted a gene-based compound heterozygosity test to reveal additional genes underlying complex diseases. With this approach applied to iron overload, a strong association signal was identified between the fibroblast growth factor–encoding gene, FGF6, and hemochromatosis in the central Wisconsin population. Functional validation showed that fibroblast growth factor 6 protein (FGF-6) regulates iron homeostasis and induces transcriptional regulation of hepcidin. …

Marine Reserves Shape Seascapes On Scales Visible From Space, Elizabeth M. P. Madin, Alastair R. Harborne, Aaron M. T. Harmer, Osmar J. Luiz, Trisha Brooke Atwood, Brian J. Sullivan, Joshua S. Madin

Watershed Sciences Faculty Publications

Marine reserves can effectively restore harvested populations, and ‘mega-reserves’ increasingly protect large tracts of ocean. However, no method exists of monitoring ecological responses at this large scale. Herbivory is a key mechanism structuring ecosystems, and this consumer–resource interaction's strength on coral reefs can indicate ecosystem health. We screened 1372, and measured features of 214, reefs throughout Australia's Great Barrier Reef using high-resolution satellite imagery, combined with remote underwater videography and assays on a subset, to quantify the prevalence, size and potential causes of ‘grazing halos’. Halos are known to be seascape-scale footprints of herbivory and other ecological interactions. Here we …

Building Consumer Trust In The Cloud: An Experimental Analysis Of The Cloud Trust Label Approach, Lisa Van Der Werff, Grace Fox, Ieva Masevic, Vincent C. Emeakaroha, John P. Morrison, Theo Lynn

Department of Computer Science Publications

The lack of transparency surrounding cloud service provision makes it difficult for consumers to make knowledge based purchasing decisions. As a result, consumer trust has become a major impediment to cloud computing adoption. Cloud Trust Labels represent a means of communicating relevant service and security information to potential customers on the cloud service provided, thereby facilitating informed decision making. This research investigates the potential of a Cloud Trust Label system to overcome the trust barrier. Specifically, it examines the impact of a Cloud Trust Label on consumer perceptions of a service and cloud service provider trustworthiness and trust in the …

Department Of Chemistry And Biochemistry 24th Departmental Symposium In Conjunction With The Petersheim Academic Exposition, Seton Hall University

Petersheim Academic Exposition

No abstract provided.

Responses Of Soil Carbon Sequestration To Climate-Smart Agriculture Practices: A Meta-Analysis, Xiongxiong Bai, Yawen Huang, Wei Ren, Mark Coyne, Pierre-Andre Jacinthe, Bo Tao, Dafeng Hui, Jian Yang, Chris Matocha

Biology Faculty Research

Climate-smart agriculture (CSA) management practices (e.g., conservation tillage, cover crops, and biochar applications) have been widely adopted to enhance soil organic carbon (SOC) sequestration and to reduce greenhouse gas emissions while ensuring crop productivity. However, current measurements regarding the influences of CSA management practices on SOC sequestration diverge widely, making it difficult to derive conclusions about individual and combined CSA management effects and bringing large uncertainties in quantifying the potential of the agricultural sector to mitigate climate change. We conducted a meta-analysis of 3,049 paired measurements from 417 peer-reviewed articles to examine the effects of three common CSA management practices …

Program And Proceedings: Nebraska Academy Of Sciences 1880–2019, 139th Anniversary Year, One Hundred-Twenty-Ninth Annual Meeting, April 12, 2019

Nebraska Academy of Sciences: Programs and Proceedings

Program

Aeronautics and Space Science

Collegiate Academy: Biology

Biological and Medical Sciences

Chemistry and Physics

Teaching and Learning the Dynamics of Cellular Respiration Using Interactive Computer Simulations Workshop

Life After College: Building Your Resume for the Future Workshop

Collegiate Academy: Chemistry and Physics

Aeronautics and Space Science, Poster Session

Maiben Memorial Lecture: David Swanson

Anthropology

Applied Science and Technology

Earth Science

Environmental Sciences

Teaching of Science and Math

Anti-Proliferative Effects Of Common Plant Extracts On Tumor Cells In Vitro, Joseph Kelly, Christopher Borchers, Perkins Aiyegbeni, Paige Boote, Brittany Buren, Katie Dekruyff, Emily Geraets, Chau Le, Rachel Mercer, Courtney Mithelman, Haley Muyskens, Renju Pun, Zachary Rosson, Sara S. Tolsma

Celebration of Research

In 2018, the World Health Organization identified cancer as responsible for 1 in 6 deaths globally, encouraging the exploration of creative forms of cancer treatment. Our research interests include identifying anti-proliferative plant extracts and illuminating mechanisms of growth inhibition. In this study we measured the effects of Vitis vinifera (grapeseed), Juniperus communis (juniper berry), and Curcuma longa (turmeric) extracts on the proliferation of several normal and tumorigenic cell strains and lines (HFF-S2, HeLa, HepG2, MDA MB 231, and RAW 264.7). We performed dose response CyQUANT assays to analyze the effects on cell growth. We are using DNA analysis, recovery experiments, …

12th Annual Research Week--Event Proceedings, Office Of Research And Sponsored Projects Operations

Excellence Summit (Research Week) Archived Proceedings

12th Annual Research Week

"A Celebration of Student Research"

The Potential Of Marine Microbes, Flora And Fauna In Drug Discovery, Santana Alexa Lavonia Thomas

USF Tampa Graduate Theses and Dissertations

Natural products are chemical compounds synthesized by a living organism. These compounds have been utilized by humans from ancient times to the present for their pharmacological and biological activities. Terrestrial organisms are considered a lucrative source of bioactive compounds and chemical diversity. Within the past 60 years, the marine environment has presented its potential as a validated source for structural diversity and biologically active compounds. Technology has given access from shallow waters down to the abyssal plane for exploration and investigation of the inhabitants. Marine organisms have displayed their benefits in the pharmaceutical industry with the approval of several marine-derived …

Relative Salt Tolerance Of Seven Japanese Spirea Cultivars, Yuxiang Wang, Liqin Li, Youping Sun, Xin Dai

Plants, Soils, and Climate Faculty Publications

Spirea (Spiraea sp.) plants are commonly used in landscapes in Utah and the intermountain western United States. The relative salt tolerance of seven japanese spirea (Spiraea japonica) cultivars (Galen, Minspi, NCSX1, NCSX2, SMNSJMFP, Tracy, and Yan) were evaluated in a greenhouse. Plants were irrigated with a nutrient solution with an electrical conductivity (EC) of 1.2 dSmL1 (control) or saline solutions with an EC of 3.0 or 6.0 dSmL1 once per week for 8 weeks. At 8 weeks after the initiation of treatment, all japanese spirea cultivars irrigated with saline solution with an EC of 3.0 dSmL1 still exhibited good or …

Sulitest®: A Mixed-Method, Pilot Study Of Assessment Impacts On Undergraduate Sustainability-Related Learning And Motivation, Alicia Mason

Faculty Submissions

A United Nations international collaboration between the Education for Sustainable Development (ESD) and the Principles for Responsible Management Education (PRME) resulted in the creation of Sulitest® (aka Sustainability Literacy Test) an open, online training and assessment tool freely available to higher education institutions globally. This study analyzes the effectiveness of the newly developed Sulitest® to not only measure sustainability literacy of higher education student populations, but also act as a catalyst for boosting affective learning outcomes by: (a) generating interest in sustainability-related issues, (b) improving sustainability-related understandings, and (c) enhancing students’ interests in the subject matter. In order to do …

Leveraging Image Analysis For High-Throughput Plant Phenotyping, Sruti Das Choudhury, Ashok Samal, Tala Awada

School of Natural Resources: Faculty Publications

The complex interaction between a genotype and its environment controls the biophysical properties of a plant, manifested in observable traits, i.e., plant’s phenome, which influences resources acquisition, performance, and yield. High-throughput automated image-based plant phenotyping refers to the sensing and quantifying plant traits non-destructively by analyzing images captured at regular intervals and with precision. While phenomic research has drawn significant attention in the last decade, extracting meaningful and reliable numerical phenotypes from plant images especially by considering its individual components, e.g., leaves, stem, fruit, and flower, remains a critical bottleneck to the translation of advances of phenotyping technology into genetic …

Putting The I In Science, Naomi Kirkvold

Honors Program: Expanded Learning Clubs

This club gets kids interested in science in ways that they may not have experienced in a classroom by doing weekly experiments.

Sensitivity Analyses For Tumor Growth Models, Ruchini Dilinika Mendis

Masters Theses & Specialist Projects

This study consists of the sensitivity analysis for two previously developed tumor growth models: Gompertz model and quotient model. The two models are considered in both continuous and discrete time. In continuous time, model parameters are estimated using least-square method, while in discrete time, the partial-sum method is used. Moreover, frequentist and Bayesian methods are used to construct confidence intervals and credible intervals for the model parameters. We apply the Markov Chain Monte Carlo (MCMC) techniques with the Random Walk Metropolis algorithm with Non-informative Prior and the Delayed Rejection Adoptive Metropolis (DRAM) algorithm to construct parameters' posterior distributions and then …

An Evaluation Of Elemental Composition Of Soils Associated With Ground Nesting Ants In The Family Formicidae In Somerset County, Maine, Haley H. Depner

All Student Scholarship

Ants are keystone organisms and engineers in many ecosystems, playing an important role in nutrient cycling and rearrangement of organic and inorganic materials through foraging, waste management and, in subterranean species, nest construction and maintenance. In this study, I conducted elemental analysis of soils for calcium, copper, iron, potassium, manganese and zinc in and around the colonies of subterranean nesting ants. The two-step analysis used in this study utilizes the efficiency of X-Ray Fluorescence (XRF) Spectrometry to look for overall trends in elemental composition followed by Flame Atomic Absorbance (AA) to achieve higher resolution of select elements of interest as …

A Pedagogical Framework For The Design And Utilization Of Place-Based Experiential Learning Curriculum On A Campus Farm, Julia L. Angstmann, Amber J. Rollings, Grant A. Fore, Brandon H. Sorge

Scholarship and Professional Work - LAS

Campus agriculture projects are increasingly being recognized as spaces impactful to student engagement and learning through curricular and co-curricular programming; however, most campus farm activities are limited to agriculture or sustainability programs and/or co-curricular student clubs. Thus, campus farms are largely underutilized in the undergraduate curriculum, marking a need to explore the efficacy and impact of engaging a diverse array of disciplinary courses in the rich social, environmental, and civic context of local sustainable agriculture. The Farm Hub program presented here incentivizes instructors to refocus a portion of existing course content around the topic of local, sustainable agriculture, and reduces …

Regenerative Grazing And The Benefits Of Livestock On Soils In Northern New South Wales, Raymond Mooney

Independent Study Project (ISP) Collection

Conventional cattle grazing has received criticism for environmental degradation in the past. Regenerative grazing and the principles of regenerative agriculture show encouraging signs that proper livestock management and planned grazing can reverse degradation and mitigate climate change. An emphasis on soil health and increasing soil carbon and organic matter levels reveals positive feedback for environmental health, the economic security of farmers, and nutritional health of consumers.

In this study I looked to investigate the benefits of regenerative agriculture, reasons why it is being practiced, and the extent it is practiced within the grazing in comparison to traditional methods within Northern …

Eating Some Invasive Species Could Help To Mitigate The Impacts Of Climate Change-Related Invasions, And May Increase Future Food Security, Jesse Bull Saffeir

Independent Study Project (ISP) Collection

Climate change is predicted to increase the spread and abundance of invasive species and to erode global food security. I hypothesized that by incorporating edible invasive species into local food sheds, these two problems could help to mitigate each other. I set out to answer two questions: could eating invasive species reduce their spread and abundance? And could eating invasive species minimize the impacts of climate-change related food shocks? To answer these questions, I surveyed the existing literature on human consumption of invasive species, created a list of criteria that make an invasive species suitable for management through human consumption, …

Snowshoe Hares (Lepus Americanus) Alter Feeding Behavior In Response To Coyote (Canis Latrans) And Moose (Alces Alces) Cues At Diverse Vegetation Densities, Zachary K. Lankist

Student Scholarship

Prey interpret predator cues as a warning and use them to assess the danger of a given area. Multiple prey species avoid chemical cues from predators at feeding sites because the risk of death outweighs the benefit of food. However, we lack information regarding avoidance of chemical cues from competitors as well as how foraging behavior changes alongside vegetative cover. To test if chemical cues and veget~tive cover alter prey vigilance, number of visits, and time spent at feeding sites, I observed snowshoe hares (Lepus americanus) in plots containing coyote (Canis latrans; predator) and moose (Alces alces; competitor) urine across …

Droughtscape, Spring 2019, Cory Matteson

DroughtScape: Quarterly Newsletter of the National Drought Mitigation Center (2007–2023)

Contents

From the Director

Wet and cool conditions bring drought relief to the West

Notable numbers from Q1

Drought impact summary, 1st quarter 2019

New research examines drought effects on state recreation areas

At Ethiopian space science workshop, NDMC climatologist sees promise in preparing for drought

U.S.- affiliated Pacific Islands now part of U.S. Drought Monitor

Water conservation tips for drought, flood and other disasters

Upcoming events

Learning From The Soil's Memory: Tailoring Of Fertilizer Application Based On Past Manure Applications Increases Fertilizer Use Efficiency And Crop Productivity On Kenyan Smallholder Farms, Samuel Njoroge, Antonius G.T. Schut, Ken E. Giller, Shamie Zingore

All Peer-Reviewed Publications

The large uncertainty in yield response to fertilizer application within smallholder cropping systems in sub-Saharan Africa (SSA) limits efforts aimed at intensifying crop production based on increased fertilizer application. We assessed the key field-scale cause of variability in maize (Zea mays) grain yield response to fertilizer nitrogen (N), phosphorus (P) and potassium (K) in the Sidindi area of Western Kenya based on past manure application, distance from the homestead, and clay and silt contents. We used data from nutrient omission trials conducted on 23 farms over seven consecutive cropping seasons covering the period 2013–2016, without changing treatments or plot location. …

The Disparity Between Scientific Consensus And American Public Opinion Of Genetically Modified Organisms And Genetic Engineering, Matthew A. Cherubino

Student Publications

Genetically modified organisms (GMOs) and genetic engineering (GE) are accepted as safe and useful by the consensus of the scientific community. Their diverse utility has shown promise in addressing major challenges of the 21st century, including world hunger, global warming, and the prevalence of diet-related diseases (e.g. heart disease, cancer, diabetes, etc.). A 2014 Pew Research Center survey revealed that while 88% of scientists agreed that GM foods were safe to eat, only 37% of American consumers agreed. Furthermore, only 35% of U.S. adults trusted scientists to accurately inform the public about GMOs. To explain this disparity, I synthesize information …

Defining, Measuring, And Applying Soil Quality: An Unresolved Debate, Salvador Ramirez Ii

Doctor of Plant Health Program: Dissertations and Student Research

Paradigm shifts occur periodically in the sciences. Proposed paradigm shifts offer opportunities to reexamine extant paradigms and evaluate new ones. Soil science experienced a controversy resembling a proposed paradigm shift in the late 1990s and early 2000s when it grappled with the idea of soil quality (SQ). The most contentious points in the SQ debates were (1) the word “quality” itself and designing a functional definition, (2) identifying appropriate soil quality indicators, (3) the integration of soil quality indicators, and (4) the interpretation of soil quality indicators. The latter three disagreements were rooted in the established, fundamental, soil science paradigm. …

Technology For Behavioral Change In Rural Older Adults With Obesity, John A. Batsis, John A. Naslund, Alexandra B. Zagaria, David Kotz, Rachel Dokko, Stephen J. Bartels, Elizabeth Carpenter-Song

Dartmouth Scholarship

Background: Mobile health (mHealth) technologies comprise a multidisciplinary treatment strategy providing potential solutions for overcoming challenges of successfully delivering health promotion interventions in rural areas. We evaluated the potential of using technology in a high-risk population.

Methods: We conducted a convergent, parallel mixed-methods study using semi-structured interviews, focus groups, and self-reported questionnaires, using purposive sampling of 29 older adults, 4 community leaders and 7 clinicians in a rural setting. We developed codes informed by thematic analysis and assessed the quantitative data using descriptive statistics.

Results: All groups expressed that mHealth could improve health behaviors. Older adults were optimistic that mHealth …

Lepidopteran Diversity And Abundance Across Five Different Indigenously Managed Land Use Zones In The Naso-Teribe Territory, Bocas Del Toro Province, Panama, Eli Dotson

Independent Study Project (ISP) Collection

Lepidopterans, the order that includes both moths and butterflies, are among the best-known insects taxonomically and ecologically due to the relative ease of monitoring them in comparison to other groups of insects. Their distribution follows that of other insects and forms of life, as around 90% of species have a tropical distribution. This incredibly speciose group of animals fills many vital roles in the ecosystems they inhabit, including herbivores in their larval stages, prey for a variety of predators in all life stages, and pollinators for many plant species as adults. In some circumstances, certain species may also serve as …

Kanjirowa Blues: An Exploration Of Environmental And Climate Consciousness In Lower Dolpa, Nepal, Casey Greenleaf

Independent Study Project (ISP) Collection

It has been scientifically demonstrated that high altitude, mountainous regions such as the Himalayas are extremely susceptible to and at accelerated risk of the effects of climate change. The regions of Lower Dolpa discussed in this work, Juphal, Dunai, Chun, and Dapu, lie in a glacial watershed, and are at present risk of landslides, floods, wildfires, and rely on agricultural and transhumant livelihoods that are uniquely susceptible to the impacts of changing temperature and weather patterns. People in this region are being forced to incrementally adapt and reframe their understanding of their surroundings due to both aforementioned severe events as …

Rising Temperatures In Hot Regions – How Many Species Would Be Able To Survive?, Sounak Ghosh

Biology Theses

Temperatures are rising throughout the world but not uniformly. Different latitudes have varying degrees of temperature rise and the most rapid changes are happening in the higher northern latitudes while only minor warming is taking place in the hot tropical regions. It has been widely assumed that the biggest ecological impacts will be seen in the cooler northern latitudes and that the impacts in hot tropical regions will be much smaller. However, if species in hot regions are already close to their thermal limits and if species in cold regions are far below them, it may be that small temperature …

Regression For Pooled Testing Data With Biomedical Applications, Juexin Lin

Theses and Dissertations

Since first introduced by Dorfman in 1943, pooled testing has been widely used as a cost and time effective testing protocol in the variety of applications. This dis- sertation consists of three projects that reveal the use of pooling techniques in the disease prevention from the perspective of regression. For disease monitoring and control, individual covariates information are often of practical interest and yield meaningful interpretations. It is natural to model the outcome of interest, which can be either a disease status (binary) or a biomarker concentration index (continuous), with individual-specific covariates through a regression analysis. Chapter 2 focuses on …

Endolithic Microbial Carbon Cycling In East Antarctica, Natalie Tyler

Theses and Dissertations

Microbes adapt to inhospitable conditions by colonizing niches that protect them from severe environmental conditions, making them model organisms in the search for life on other planets. Endoliths are globally ubiquitous microbes that colonize structural cavities within rocks, which protect them from environmental stressors while maintaining access to nutrition and light sources. Despite harsh katabatic winds, extreme temperature fluctuations, harmful UV radiation and exposure to desiccation, endolithic communities have been observed in Antarctica. Previous studies have found viable and abundant microbial biomass through microscopy and the addition of isotopic labels. However, due to the slow metabolic activity of these microbes, …

Wolves Are Wild: A Collection Of Narratives About Rescued Wolves And Wolfdogs, Molly G. Vorhaus

Student Publications

Breeders across the country are creating wolfdogs by breeding dogs (Canis lupus familiaris) with wolves (Canis lupus) for a large profit. This project is a response to the growing exotic pet trade of wolves and wolfdogs. Through this project, I hope to bring awareness to the issues associated with these animals being raised in captivity. Recent research has shown that raising a wolf or wolfdog in captivity can lead to various negative psychological and physical effects on the animal, and can cause potential problems for humans as well. This practice is embedded in the concept of humans wanting to own …