Digital Commons Network^™

Identification Of A Complex Associated With Processing And Polyadenylation In Vitro Of Herpes Simplex Virus Type 1 Thymidine Kinase Precursor Rna., Fang Zhang, Charles N. Cole

Dartmouth Scholarship

Cleavage and polyadenylation of substrate RNAs containing the herpes simplex virus type 1 (HSV-1) thymidine kinase (tk) gene polyadenylation signal region were examined in HeLa cell nuclear extract. 3'-End RNA processing was accurate and efficient and required ATP and Mg2+. Cleavage, but not polyadenylation, occurred in the presence of EDTA or when ATP was replaced with 3' dATP (cordycepin) or AMP(CH2)PP, a nonhydrolyzable analog of ATP. Processing in vitro and in vivo showed the same signal element requirements: a series of substrates containing linker scanning, internal deletion, and small insertion mutations was processed with the same relative efficiencies and at …

[Book Review Of] Two Reviews Of: The Nazi Doctors, By Robert J. Lifton, Robert Barry, Eugene F. Diamond

The Linacre Quarterly

No abstract provided.

[Book Review Of] Taking Care: Supporting Older People And Their Families, By Nancy R. Hooyman And Wendy Lustbader, Robert J. Barnet

The Linacre Quarterly

No abstract provided.

The Correlation Of Physiological Responses To Verbal Behavior During Environmental Stimuli Exposure, Cynthia Lou Baker

Graduate Theses

The study was designed to investigate the hypothesis that various environmental stimuli would elicit specific individual responses, as reflected in autonomic patterns. It then became the aim of the study to see whether the stimuli which controlled the physiological pattern would also control the verbal response. Three female and two male students volunteered for the single-subject experiment, where they were exposed to a 15-slide sequence of environment stimuli. Results indicated that when allowed to verbally respond without rating restrictions, responses varied greatly, limiting the possibilities of comparison. In kind, the physiological responses exhibited great variability. Results also supported the position …

Tubulin Interaction With Kinetochore Proteins: Analysis By In Vitro Assembly And Chemical Cross-Linking, R. D. Balczon, B. R. Brinkley

University Faculty and Staff Publications

The sera from patients with the CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia) variation of the autoimmune disease scleroderma contain autoantibodies that specifically recognize the kinetochore by immunofluorescence. Two major antigens of molecular masses 18 and 80 kD are consistently identified by Western blotting of proteins of isolated chromosomes using CREST sera. In this paper, the possible roles that these two proteins play in the interaction of metaphase chromosomes with tubulin and microtubules are examined using two different procedures. In one set of experiments. Chinese hamster ovary (CHO) chromosomes were extracted with 1-2 M NaCl before incubating with phosphocellulose-purified …

Update - August 1987, Loma Linda University Center For Christian Bioethics

Update

In this issue:

-- Harvey Cox launches Adventism and Ethics series
-- Society for bioethics consultation schedules three conferences
-- Conference probes "Humanity" of residency programs

[ Active Voluntary Euthanasia: Is It Moral? ]
-- Yes!
-- No!
-- Discussion

Perceived Job Stress In Critical Care Nursing In A Military Setting, Jeannette Theriault

Ellmer School of Nursing Theses & Dissertations

The purposes of this study. were: to explore job-related perceptions of stress in a group of military critical care nurses; and to determine if differences in anxiety and stress levels existed between nurses working in coronary care (CCCU) and medical/surgical intensive care (ICU) settings using the State/Trait Anxiety Inventory and the Questionnaire of Stressful Factors in Intensive Care. A free response question and a force-response checklist were also used to identify and rank work-related stressors. The sample of Navy nurses (n=105) consisted of 39 CCU nurses, 62 medical/surgical ICU nurses and 4 critical care supervisors. The results of the mailed …

The Anatomical Relationship Of The Mandibular Canal And Related Structures In Immature And Mature Dried Mandibles, Dale Denio

Loma Linda University Electronic Theses, Dissertations & Projects

The anatomical relationship of the mandibular canal and related structures were examined in 29 dried human mandibles (21 mature and eight immature). First and second premolars and molars were sectioned in a buccal-lingual plane. The molars were separated into mesial and' distal sections. A total of 322 sections were examined according to age (immature or mature), left or right, and type of tooth/root. Thirteen measurements were taken of each section and the results were statistically analysed.

The results indicated no significant difference between the mean averages when comparing the 13 data points between the left and right side of mandibles …

A Transmission Electron Microscopy Study Of Early Changes In Hypoxic Pulps, Nah Nah Chen

Loma Linda University Electronic Theses, Dissertations & Projects

Early cellular changes in pulps whose blood supplies were severed were studied using transmission electron microscopy (TEM). Twenty-eight extracted teeth were divided into seven equal groups. Except for the 0 hour group, all other teeth were incubated in Tyrode solution at 37°C, after extraction. After 1/4, 1/2, 1, 2, 4, and 8 hours, teeth were split open and the pulps were processed for light and transmission electron microscopy and evaluated for cellular changes. Except for nuclear pyknosis in the 4 and 8 hour groups, light microscopic examination revealed very little changes when compared with that of TEM examination. With the …

Agentive Theory As Therapy: An Outcome Study, Daniel K. Judd

Theses and Dissertations

The present study evaluated the efficacy of a four-week seminar which emphasized the principles of Agentive Theory. This theory, which is compatible with theories of a phenomenological/ existential perspective, was first developed by C. T. Warner, an American philosopher. Agentive Theorists/Therapists emphasize that our negative emotions, ie., depression, anger, etc. , are assertions or judgments we make and not merely feelings we are responsible for controlling or expressing. Forty-eight outpatients who sought help with personal/emotional problems from a department of behavioral medicine were assigned to either a treatment or waiting-list control Group. Following a four-week treatment phase, the treatment group …

Distribution Of Vertebrates Of The Bighorn Canyon National Recreation Area, Stanley H. Anderson, Wayne A. Hubert, Craig Patterson, Alan J. Redder, David Duvall

Great Basin Naturalist

During a survey of the vertebrates in the Bighorn Canyon National Recreation Area, 46 mammal, 210 bird, 9 reptile, 5 amphibian, and 28 fish species were found. Habitat structure and moisture were two environmental variables associated with species numbers across this 1,820-ha area. Eight distinct habitats were evaluated. In coniferous forests, 27% of the birds and 54% of the mammals were observed. Sagebrush/grasslands and upland shrublands were very arid and had relatively few vertebrates. Five introduced game birds occurred in sagebrush habitat. Riparian and wetland habitats had the largest number of unique vertebrates, 38 and 77 respectively. The establishment of …

A Novel Form Of Human Polymorphism Involving The Hdhfr-Ψ1 Pseudogene Identifies Three Rflps, Nicholas P. Anagnou, S. E. Antonarakis, Stephen J. O'Brien, Arthur W. Nienhuis

Biology Faculty Articles

No abstract provided.

Vital Signs, Summer 1987, Boonshoft School Of Medicine

Vital Signs

A thirty five page newsletter created by the Boonshoft School of Medicine to document the current affairs of the school. This issue includes a variety of feature articles, alumni profiles, class notes, and more.

Craniofacial Morphology And Occlusal Variation In Monozygous And Dizygous Twins, William K. Lobb

School of Dentistry Faculty Research and Publications

A study of 60 pairs of twins, 30 monozygous and 30 dizygous, finding a strong genetic component overlaid by functional adaptation most prominent in the dental area.

Reactions Of Organic N-Chloramines In The Gastric Fluid Of The Rat, Kathryn E. Mazina

Chemistry & Biochemistry Theses & Dissertations

Using chlorine as a drinking water disinfectant may have potential health effects due to its reactivity with organic amino nitrogen compounds found in the stomach. Organic N-chloramines have been shown to form in the stomachs of laboratory rats. The possible reactions of N-chloramines in the stomach fluid were examined in this study using a model radiolabeled N-chloramine. ³⁶Cl-N-Chloropiperidine, was synthesized and purified to remove ³⁶Cl-chloride. Stomach fluid was obtained from Sprague-Dawley rats which had been first fasted for 24 or 48 hours and then administered 3 mL of deionized water. Different concentrations of radiolabeled chloramine were reacted with …

Implications Of A Family Systems Orientation For Preventing Psychiatric Hospitalization, James Howard Bullock

Psychology Theses & Dissertations

The present study evaluated the implications of a family systems approach for preventing psychiatric hospitalization by comparing the behavior of field clinicians who professed a systems view versus clinicians who held more traditional nonsystems views. The focus of the investigation was the clinical assessments and decisions of 31 clinicians who worked at five Virginia public mental health centers as they prescreened (i.e., evaluated need for inpatient treatment) 171 candidates for hospitalization. Q-technique was employed to determine theoretical orientation.

It was hypothesized that clinicians who held a systems view would be more likely than nonsystems therapists to evaluate and attempt to …

Jefferson Alumni Bulletin – Volume Xxxvi, Number 4, Summer 1987

The Bulletin (formerly the Jefferson Medical College Alumni Bulletin)

Jefferson Alumni Bulletin – Volume XXXVI, Number 4, Summer 1987

Reunions ‘87, Page 2

Jefferson scene, Page 19

Class notes, Page 27

Senior special, Page 42

Department Of Radiology-Annual Report-July 1, 1986 To June 30, 1987, David C. Levin

Department of Radiology Administrative Documents and Reports

Department of Radiology Annual Report, July 1, 1986 to June 30, 1987. Thomas Jefferson University Hospital, Philadelphia,Pennsylvania, United States. 106 pages.

Plasma And Tumor Levels Of Somatostatin (Srif) And Somatostatin Immunochemistry In Medullary Thyroid Carcinoma: Apparently Discrepant Preliminary Results, B. Franc, J. A. Chayvialle, E. Modigliani, C. Calmettes, B. Caillou, N. Dutrieux-Berger, C. Houdent, M. Kujas

Henry Ford Hospital Medical Journal

Eighteen cases of medullary thyroid carcinoma (MTC) (11 hereditary) had parallel measurements of basal plasma levels of somatostatin (SRIF), levels of SRIF extracted from fresh tumor tissue (radioimmunoassay), and immunohistochemistry (peroxidase-antiperoxidase method) using an anti-SRIF antibody on paraffin sections. Immunohistochemistry disclosed some cell positivity in nine cases (50%). Plasma levels of SRIF were above normal (> 50 pg/mL expressed in terms of the 14 amino acid SRIF peptide) in only two of 12 patients. Extraction of fresh tumor tissue detected SRIF in 60% of our cases: two patients > l.200pg/mg (equivalents 14). with the other positive values ranging between 13 and …

Immunohistochemistry In Medullary Thyroid Carcinoma: Prognosis And Distinction Between Hereditary And Sporadic Tumors, B. Franc, B. Caillou, A. M. Carrier, N. Dutrieux-Berger, J. Floquet, M. Houcke, E. Justrabo, F. Lange, A. Pages, C. Rigaud, A. Schwartz, M. Viennet, M. F. Lebodic

Henry Ford Hospital Medical Journal

In this retrospective study of 68 cases of medullary thyroid carcinoma (MTC), morphological features were studied, which could be helpful in prognosis and in the distinction between hereditary and sporadic forms of the disease. Necrosis within the tumors seemed to carry a poor prognosis. Bilateral MTC. unquestionable C-cell hyperplasia, glandular or follicular pattern, and thyroglobulin-positive cells seemed to be more prevalent in hereditary forms of the disease. A smaller proportion of calcitonin-immunoreactive cells were encountered in hereditary tumors than in sporadic tumors.

Different Mechanisms Of Calcitonin, Calcitonin Gene-Related Peptide, And Somatostatin Regulation By Glucocorticoids In A Cell Culture Of Human Medullary Thyroid Carcinoma, Gilbert J. Cote, Robert F. Gagel

Henry Ford Hospital Medical Journal

We have employed the TT cell line, a model for the human medullary thyroid carcinoma cell, lo study the regulation of peptide hormone production by glucocorticoids. Complementary DNA probes were used to measure the calcitonin (CT), CT gene-related peptide (CGRP), and somatostatin (SRIF) mRNA levels. Dose-response experiments in serum-free medium showed that dexamethasone (six-day treatment) lowered somatostatin (to 1% of basal) and CGRP mRNA (to 50% of basal) and stimulated CT mRNA (threefold to thirteenfold) with a half-maximal effective concentration of 10⁻⁸ M. Time course studies for cells continuously exposed to 10⁻⁶ M dexamethasone showed a rapid (within …

The Second International Workshop On Multiple Endocrine Neoplasia Type 2 Syndromes, Bruce A. J. Ponder, Charles E. Jackson

Henry Ford Hospital Medical Journal

No abstract provided.

Calcitonin Gene-Related Peptide And Calcitonin In Men-2 And Sporadic Pheochromocytomas: An Immunohistochemical Study, Lis Johannsen, Henrik Daa Schroder, Soren Schifter

Henry Ford Hospital Medical Journal

Ten pheochromocytomas (five from patients with multiple endocrine neoplasia type 2 and five sporadic) were stained immunohistochemically with antibodies to calcitonin (CT) and calcitonin gene-related peptide (CGRP) by means of the peroxidase-antiperoxidase method. CGRP positive cells were found in variable numbers in all of the investigated tumors. No demonstrable difference was noted between the hereditary tumors and the sporadic tumors. Staining with CT antibody also showed cells with a positive reaction in eight of ten tumors. The distribution of the two peptides was similar, and in some cells their coexistence was visualized. Pheochromocytomas thus represent an alternative site to medullary …

Clinical Value Of Calcitonin And Carcinoembryonic Antigen Doubling Times In Medullary Thyroid Carcinoma, Charles E. Jackson, Robert A. Norum, Gary B. Talpos, Carolyn S. Feldkamp, Armen H. Tashjian Jr.

Henry Ford Hospital Medical Journal

Doubling times of basal and stimulated calcitonin (CT) levels and of random carcinoembryonic antigen (CEA) levels were compared over time in 11 patients with residual medullary thyroid carcinoma (MTC)—five with the sporadic MTC and six with the hereditary MTC. Four patients with an indolent form of the disease showed little change in CT levels over three to eight years and little change in CEA doubling times (42 to 70+ months). Seven patients showing rapid progression of disease had CT doubling times of three to 11 months and CEA doubling times of two to 12 months (however, one patient showed no …

In Vitro Secretion Of Peptides Of The Calcitonin Family: Calcitonin, Katacalcin, And Calcitonin Gene-Related Peptide, Friedhelm Raue, Hubert Serve, Eckard Rix, Reinhard Ziegler

Henry Ford Hospital Medical Journal

A tissue culture explant and a primary cell culture of malignant C-cells have been estabUshed to study the secretion pattern of calcitonin (CT), katacalcin (KC), and CT gene-related peptide (CGRP) in vitro under different conditions. Within the first five days of culture the spontaneous secretion of the peptides in both systems dropped to and remained at a constant level. With increasing calcium (Ca) concentration in the medium, a dose dependent release of CT, KC and, in cell culture, of CGRP was observed. BAY-K-8644. an analog of the Ca channel blocker nifedipine, enhanced the CT secretion to the same extent as …

Localization Of A Radiolabeled Monoclonal Antibody To Calcitonin In Rat Medullary Thyroid Carcinoma Allografts, Naguib A. Samaan, Kuo-Pao Yang

Henry Ford Hospital Medical Journal

A radiolabeled anticalcitonin monoclonal antibody (MoAb) was testedfor tumor-localizing capability in WAG/Rij rats allografted with cultured medullary thyroid carcinoma cells (line 44-2). 131I-labeled anticalcitonin IgG was taken up by the tumors in a time-dependent and dose-dependent manner. Tumor tissue uptake of the injected MoAb was 0.7 to 1.3%/g of tumor tissue when tested with a dose range of 40 lo 250 μg/kg of body weight. Distribution ratios of the radiolabeled MoAb were 1.2:1 for tumor-to-blood and 2-20:1 for tumor-to-organs. In rats injected with control IgG, disiribution ratios of radioactivity were 0.4:1 for tumor-to-blood, and 0.6-2:1 for tumor-to-organs. These results suggested …

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Regulation Of Lymphokine Production And Human T Lymphocyte Activation By 1,25-Dihydroxyvitamin D3. Specific Inhibition At The Level Of Messenger Rna., William F.C. Rigby, Sylvia Denome, Michael W. Fanger

Dartmouth Scholarship

The steroid hormone, 1 alpha,25-dihydroxyvitamin D3 (calcitriol), has been shown to inhibit T cell proliferation, primarily through inhibition of interleukin 2 (IL-2) production. In these experiments, we show that calcitriol also markedly inhibited production of the lymphokine, gamma interferon (IFN-gamma), by activated human T lymphocytes. Regulation of both IL-2 and IFN-gamma production as well as transferrin receptor (TfR) expression by calcitriol was apparent at the messenger RNA (mRNA) level as determined by Northern blotting. The decrease in IL-2 and IFN-gamma mRNA that occurred with calcitriol treatment was coordinate and not apparent up to 12 h after phytohemagglutinin stimulation, whereas decreased …

Commencement Program 1987, Loma Linda University

Commencement Programs

CONTENTS

2 | 1987 Events of Commencement

3 | Academic Procession

4 | The Speakers

7 | The Honorees

Order of Programs

• School of Dentistry, 12
• School of Medicine, 18
• Graduate School, 24
• School of Nursing, 29
• College of Arts and Sciences, School of Business and Management, and School of Education, 33
• School of Allied Health Professions, 44
• School of Health, 49

Magnetic Forces For Intrusion Of Buccal Segments In Dolichofacial Individuals With Anterior Open Bite : A Report Of Six Cases, Rosa Maria I. Reimann-Blaseio

Loma Linda University Electronic Theses, Dissertations & Projects

The correction of anterior open bite in dolichofacial individuals with magnetic forces has been studied. The attempt was made to intrude buccal segments by using interarch magnets set in repulsion in order to correct the anterior open bite of six dolichofacial patients. It was expected to intrude the posterior sections with these magnetic forces, to autorotate the mandible and to close the anterior open bite by decreasing the lower facial height and rotating the facial axis closed. The magnets were placed in each arch only on those teeth that needed intrusion, mainly second premolars and existing molars, for a period …