Digital Commons Network^™

Bcl-2 Protein Expression Is Widespread In The Developing Nervous-System And Retained In The Adult Pns, Diane E. Merry, Deborah J. Veis, William F. Hickey, Stanley J. Korsmeyer

Dartmouth Scholarship

Cell death is a common feature of neural development in all vertebrates. The bcl-2 proto-oncogene has been shown to protect a variety of cell types from programmed cell death. We have examined the distribution of bcl-2 protein in the developing and adult nervous systems. bcl-2 protein is widespread during embryonic development. Proliferating neuroepithelial cells of ventricular zones as well as the postmitotic cells of the cortical plate, cerebellum, hippocampus and spinal cord express bcl-2. Postnatally, bcl-2 is principally retained in the granule cells of the cerebellum and dentate gyrus of the hippocampus. bcl-2 expression in the CNS declines with aging. …

Separate Metabolic Pathways Leading To Dna Fragmentation And Apoptotic Nuclear Chromatin Condensation, D. Sun, Shibo Jiang, Li-Mou Zheng, David M. Ojcius, John Ding-E. Young

All Dugoni School of Dentistry Faculty Articles

Apoptosis is the predominant form of cell death observed in a variety of physiological and pathological conditions such as cancer involution, insect metamorphosis, the development of the immune and nervous systems, and embryogenesis. The typical nuclear changes taking place in apoptotic cells include extensive condensation of chromatin and internucleosomal DNA fragmentation into units of 200 base pairs. However, the mechanisms responsible for both chromatin condensation and DNA fragmentation have yet to be elucidated. In this study, micrococcal nuclease and the divalent cations, Ca2+ and Mg2+, were applied to isolated nuclei in an attempt to reconstitute in vitro the digestion of …

Cd40 Ligand Expression Is Defective In A Subset Of Patients With Common Variable Immunodeficiency., Mary Farrington, Laura S. Grosmaire, Shigeaki Nonoyama, Susanna H. Fischer, Diane Hollenbaugh, Jeffrey A. Ledbetter, R. J. Noelle

Dartmouth Scholarship

Common variable immunodeficiency (CVI) is characterized by hypogammaglobulinemia and recurrent bacterial infections due to failure of CVI B cells to differentiate in vivo into immunoglobulin-secreting plasma cells. We hypothesized that T-cell dysfunction resulting in abnormal contact-mediated B-cell activation may play a prominent role in the failure of CVI B cells to produce specific antibody. We have previously shown that B-cell proliferation and IgE production after stimulation with anti-CD40 and interleukin (IL) 4 were normal in 22 CVI patients evaluated, indicating that CVI B cells respond to signals delivered via CD40. Here we report that CD40 ligand (gp39) mRNA expression by …

Caduceus 1994, Stritch School Of Medicine

Stritch School of Medicine Yearbooks

No abstract provided.

Quantitative Economic Evaluations Of Hiv-Related Prevention And Treatment Services: A Review, David R. Holtgrave, Ronald O. Valdiserri, Gary A. West

RISK: Health, Safety & Environment (1990-2002)

Dr. Holtgrave and colleagues at the CDC set forth an extensive taxonomy of HIV prevention and treatment services and review reports of efforts to subject some of those services to formal economic evaluation. They find few services thus far to have been so evaluated, no evaluation to have focused solely upon behavioral outcomes and most economic evaluations to lack formal quantitative analyses.

Coping With The Risk Of Cancer In Children Living Near Power Lines, Eileen N. Abt

RISK: Health, Safety & Environment (1990-2002)

Ms. Abt briefly summarizes evidence linking power lines with a possible increase in risk of childhood cancer. She also recounts how, although many experts remain skeptical of causality, public fears, whether or not warranted, have themselves created serious problems. Finally, she proposes ways to address those problems.

Review Of: The Ethics Of Reproductive Technology (Kenneth D. Alpern Ed., Oxford University Press 1992), Rochelle S. Ferber

RISK: Health, Safety & Environment (1990-2002)

Review of: The Ethics of Reproductive Technology (Kenneth D. Alpern ed., Oxford University Press 1992). Additional readings, glossary, introduction, notes, preface. LC 92-8252; ISBN 0-19-507435-1. [370 pp. Paper $19.95. 200 Madison Avenue, New York NY 10016.]

Review Of: National Research Council, Issues In Risk Assessment, Diane M. Albert

RISK: Health, Safety & Environment (1990-2002)

Review of: National Research Council, Issues in Risk Assessment (National Academy Press). Appendices, executive summary, figures, preface, references, tables. LC 92-61838; ISBN 0-309-04786-2. [374 pp. Paper $37.50 - S&H, $4 for first and $0.50 for each additional copy. 2101 Constitution Avenue, NW, Washington DC 20418.]

Lipopolysaccharide-Reactive Immunoglobulin E Is Associated With Lower Mortality And Organ Failure In Traumatically Injured Patients, Joseph T. Dipiro, Robert G. Hamilton, Thomas R. Howdieshell, N. Franklin Adkinson Jr., Arlie R. Mansberger Jr.

Publications from the Office of the Dean

Antilipopolysaccharide (anti-LPS) immunoglobulin G (IgG) and IgM have been associated with protection from LPS effects in vivo. We investigated the presence of IgE and anti-LPS in 32 patients that had experienced severe traumatic injury and in 35 healthy volunteers; we also investigated whether IgE anti-LPS was associated with important clinical events. Plasma samples were collected daily from patients in the intensive care unit and on one occasion from volunteers; the samples were assayed for IgE anti-LPS. IgE anti-LPS was assayed by enzyme-linked immunosorbent assay with monoclonal anti-human IgE as the capture antibody. Detection was accomplished with biotin-labeled LPS (Escherichia coli …

Reactive Oxygen-Dependent Dna Damage Resulting From The Oxidation Of Phenolic Compounds By A Copper-Redox Cycle Mechanism, Y. Li, M. A. Trush

Osteopathic Medicine, Jerry M. Wallace School of

No abstract provided.

Ada News Daily - 1994 Day 1, American Dental Association, Publishing Division

ADA News

The ADA News Daily (also called the ADA News Convention Daily) is a special edition of the ADA News published each day during American Dental Association Annual Sessions.

Ada News Daily - 1994 Day 3, American Dental Association, Publishing Division

ADA News

The ADA News Daily (also called the ADA News Convention Daily) is a special edition of the ADA News published each day during American Dental Association Annual Sessions.

Cloning Of The Cellular Receptor For Amphotropic Murine Retroviruses Reveals Homology To That For Gibbon Ape Leukemia Virus, Daniel G. Miller, Robert H. Edwards, A. Dusty Miller

Biomedical and Pharmaceutical Sciences Faculty Publications

The host and tissue specificity of retrovirus infection is largely determined by specific cellular receptors that mediate virus entry. Genes encoding these receptors are widely distributed in the genome, and the receptors identified to date show no sequence similarity. We have identified the cellular receptor for amphotropic murine retroviruses, Ram-1, by screening a rat cDNA expression library introduced into amphotropic virus-resistant hamster cells. The 656-amino acid receptor is homologous to the gibbon ape leukemia virus receptor at both hydrophobic termini but is highly divergent in the central hydrophilic region. Both receptors appear to be integral membrane proteins having multiple membrane-spanning …

Control Of Cationic Amino Acid Transport And Retroviral Receptor Functions In A Membrane Protein Family, Michael Kavanaugh, Hao Wang, Zheng Zhang, Weibin Zhang, Yan-Na Wu, Esther Dechant, R. Alan North, David Kabat

Biomedical and Pharmaceutical Sciences Faculty Publications

A partial cDNA sequence indicated that the T lymphocyte early-activation gene (Tea) encodes a protein related to the dual-function ecotropic retrovirus receptor/cationic amino acid transporter (ecoR/CAT1), and RNA blots suggested highest Tea expression in T lymphocytes and liver (MacLeod, C.L., Finley, K., Kakuda, D. Kozad, C.A., and Wilkinson, M.F. (1990) Mol. Cell. Biol. 7, 3663-3674). The sequence of full-length Tea cDNA from liver (3683 bases) predicts a 657-amino-acid protein (CAT2 alpha) with 12-14 transmembrane domains. A long (515 base) region with six initiation codons and termination codons precedes the translation start codon. The liver Tea cDNA is identical to Tea …

Functional Expression Of Two Glucose Transporter Isoforms From The Parasitic Protozoan Leishmania Enriettii, Chris K. Langford, Brian M. Little, Michael Kavanaugh, Scott M. Landfear

Biomedical and Pharmaceutical Sciences Faculty Publications

The parasitic protozoan Leishmania enriettii contains a family of tandemly repeated genes, designated Pro-1, that encode proteins with significant sequence similarity to mammalian facilitative glucose transporters. Pro-1 mRNAs are expressed almost exclusively in the promastigote or insect stage of the parasite life cycle. The Pro-1 tandem repeat encodes two isoforms of the putative transporter, iso-1 and iso-2, which have identical predicted amino acid sequences except for their NH2-terminal hydrophilic domains. We have now expressed both iso-1 and iso-2 by microinjecting their RNAs into Xenopus oocytes and assaying these oocytes for transport of various radiolabeled ligands. Both iso-1 and iso-2 transport …

Cell-Surface Receptors For Gibbon Ape Leukemia Virus And Amphotropic Murine Retrovirus Are Inducible Sodium-Dependent Phosphate Symporters, Michael Kavanaugh, Daniel G. Miller, Weibin Zhang, Wendy Law, Susan L. Kozak, David Kabat, A. Dusty Miller

Biomedical and Pharmaceutical Sciences Faculty Publications

Cell surface receptors for gibbon ape leukemia virus (Glvr-1) and murine amphotropic retrovirus (Ram-1) are distinct but related proteins having multiple membrane-spanning regions. Distant homology with a putative phosphate permease of Neurospora crassa suggested that these receptors might serve transport functions. By expression in Xenopus laevis oocytes and in mammalian cells, we have identified Glvr-1 and Ram-1 as sodium-dependent phosphate symporters. Two-electrode voltage-clamp analysis indicates net cation influx, suggesting that phosphate is transported with excess sodium ions. Phosphate uptake was reduced by > 50% in mouse fibroblasts expressing amphotropic envelope glycoprotein, which binds to Ram-1, indicating that Ram-1 is a major …

College Of Optometry Student Handbook, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

University Of Texas At Tyler Catalog, 1994-1996, University Of Texas At Tyler

Catalogs

Archives Space instance: https://archivesspace.uttyler.edu/repositories/2/archival_objects/22365

Outlook Magazine, Fall 1994

Outlook Magazine

No abstract provided.

Poststroke Depression, Colleen Frohberg

Physical Therapy Scholarly Projects

Depressed mood is the most common psychiatric disorder following stroke with estimated prevalence rates ranging anywhere from 20% to 60%. It has been reported that effective and rapid treatment of poststroke depression may enhance stroke rehabilitation and speed up functional recovery. The potential reversibility of depression emphasizes the importance of early identification of poststroke depression. Yet, poststroke depression remains largely untreated as part of the rehabilitation process. Therefore, it is important that health professionals, such as physical therapists, who often work with patients who have suffered a stroke are aware of the signs and symptoms of poststroke depression to ensure …

Optimizing Conditions For Cloning Cdna From Chicken Brain, Stephen Clark

Masters Theses & Specialist Projects

Acoustic over stimulation can lead to hair cell loss in the auditory epithelium. Hair cell loss or damage in mammals often results in decreased auditory sensitivity which is irreversible. However for birds, hair cells which are damaged by acoustic trauma or ototoxic drugs may be replaced by regeneration. As the first step in determining the molecular mechanisms of hair cell regeneration, gene expression techniques including cDNA synthesis and cloning were attempted first with the chicken brain. The initial attempts at making cDNA libraries were elusive; therefore, experiments were performed to identify problems and enhance the efficiency of specific steps. These …

Evaluation Of A Nursing Residency Program, Michele Marie Bird

Theses Digitization Project

Recruitment and retention of professional nurses are crucial issues for hospital departments of nursing. Recognizing the necessity to bridge the gap that persists between nursing education and nursing services, hospitals have designed programs to assist new nurses make the transition to current nursing practice. By helping individuals make the transition to current nursing practice it is hoped that staff nurses will be retained.

Funding Opportunities For Young Investigators In Radiology, Robert W. Holden, Teresa Williams

Scholarship and Professional Work

For many investigators in radiology, one of the most frustrating steps of conducting research is the search for funding sources.

Concepts Of Animal Well-Being And Predicting The Impact Of Procedures On Experimental Animals, D. J. Mellor, C. S. W. Reid

Experimental Research and Animal Welfare Collection

1. We argue that:

• in their application to non-human animals, 'welfare' and 'well-being' are interchangeable words; and that

• good welfare/well-being is the state of being manifest in an animal when its nutritional, environmental, health, behavioural and mental needs are met.

2. These latter are essentially the 'five freedoms' formulated by the Farm Animal Welfare Council of the United Kingdom.

3. Using the five freedoms as a basis, we have developed a system for assessing the impact of a proposed animal experiment or usage. The freedoms are now transformed into 'domains of potential compromise' and are redefined better to …

Burn Care: The Use Of Cpm, Electrical Stimulation And Lasers, Jennifer Walter

Physical Therapy Scholarly Projects

p>The treatment of patients with burns has always been a tedious venture. The burn team may devote many long and painful hours to the patient's rehabilitation. Physical therapists play an important part on this team, especially in the areas of wound care and pain control. The purpose of this literature review is to analyze the physiological mechanisms of continuous passive motion, electrical stimulation, and lasers in regard to wound healing and pain perceptions.

In relation to wound healing, electrical stimulation and lasers were found to enhance the actual healing process, mostly in the early phases of healing. Continuous passive …

Serologic And Genetic Identification Of Peromyscus Maniculatus As The Primary Rodent Reservoir For A New Hantavirus In The Southwestern United States, James E. Childs, Thomas G. Ksiazek, Christina F. Spiropoulou, John W. Krebs, Sergey Morzunov, Gary O. Maupin, Kenneth L. Gage, Pierre E. Rollin, John Sarisky, Russell E. Enscore, Jennifer K. Frey, C. J. Peters, Stuart T. Nichol

Zoonotics and Wildlife Disease: Publications

An outbreak of hantavirus pulmonary syndrome(H PS) in the southwestern United States was etiologically linked to a newly recognized hantavirus. Knowledge that hantaviruses are maintained in rodent reservoirs stimulated a field and laboratory investigation of 1696 small mammals of 31 species. The most commonly captured rodent, the deer mouse( Peromyscus maniculatus), had the highest antibody prevalence(3 %)to four hantavirus antigens. Antibody also was detected in 10 other species of rodent and in 1 species of rabbit. Reverse transcriptase-polymerase chain reaction( RT-PCR)products of hantavirus from rodent tissues were indistinguishable from those from human H PS patients. More than 96% of …

White-Tailed Deer As A Potential Reseroir Of Ehrlichia Spp., Jacqueline E. Dawson, James E. Childs, Kristine L. Biggie, Charla Moore, David Stalknecht, John Shaddock, John Bouseman, Erik Hofmeister, James G. Olson

Zoonotics and Wildlife Disease: Publications

We determined the antibody prevalence to Ehrlichia spp., in white-tailed deer (Odocoileus virginianus) and the geographic distribution of seropositive animals in 84 counties in Alabama, Arkansas, Florida, Georgia, Illinois, Kentucky, Louisiana, Maryland, Massachusetts, Mississippi, Missouri, North Carolina, South Carolina, Tennessee, Texas, Virginia, and West Virginia (USA). Using an indirect fluorescent antibody test we detected antibodies (≥ 1:128) to this bacterium in 544 (43%) of 1269 deer. Presence of antibodies to Ehrlichia spp. was related to a southerly latitude, low elevation, and resulting milder climatic conditions. It appears that whitetailed deer were naturally infected with Ehrlichia spp.; the infection …

Expression Of Functional Protease And Subviral Particles By Vaccinia Virus Containing Equine Infectious Anaemia Virus Gag And 5' Pol Genes, Travis C. Mcguire, Katherine I. O'Rourke, Timothy V. Baszler, Steven R. Leib, Alberta L. Brassfield, William C. Davis

Zoonotics and Wildlife Disease: Publications

Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.

Scid Mouse Spleen Does Not Support Scrapie Agent Replication, Katherine I. O'Rourke, T. P. Huff, C. W. Leathers, M. M. Robinson, J. R. Gorham

Zoonotics and Wildlife Disease: Publications

BALB/c and severe combined immunodeficiency (SCID) mice were inoculated intracerebrally or intraperitoneally with scrapie agent strain ME7 to examine the role of functional lymphocytes and follicular dendritic cells in splenic infectivity and PrP^sc accumulation. Intracerebrally inoculated BALB/c and SCID mice developed the clinical signs and microscopic lesions characteristic of scrapie. Spleens from terminally affected BALB/c mice contained PrP^sc which was detectable by immunoblot analysis; SCID mouse spleens did not contain detectable PrP so. SCID mouse spleens collected during the first 90 days after intraperitoneal infection contained neither infectivity nor PrP^sc.

The Assessment And Recognition Of Childhood Abuse Among Former Patton State Hospital Patients By Psychiatric Social Workers, Bonnie A. Criner, Hope M. Young

Theses Digitization Project

No abstract provided.