Digital Commons Network^™

A Nuclear Rna Degradation Code Is Recognized By Paxt For Eukaryotic Transcriptome Surveillance, Lindsey V Soles, Liang Liu, Xudong Zou, Yoseop Yoon, Shuangyu Li, Lusong Tian, Marielle Valdez, Angela M Yu, Hong Yin, Wei Li, Fangyuan Ding, Georg Seelig, Lei Li, Yongsheng Shi

Faculty, Staff and Students Publications

The RNA exosome plays critical roles in eukaryotic RNA degradation, but how it specifically recognizes its targets remains unclear. The poly(A) tail exosome targeting (PAXT) connection is a nuclear adaptor that recruits the exosome to polyadenylated RNAs, especially transcripts polyadenylated at intronic poly(A) sites. Here, we show that PAXT-mediated RNA degradation is induced by the combination of a 5' splice site (ss) and a poly(A) junction (PAJ) but not by either sequence alone. These sequences are bound by U1 small nuclear ribonucleoprotein particle (snRNP) and cleavage/polyadenylation factors, which, in turn, cooperatively recruit PAXT. As the 5' ss-PAJ combination is typically …

Dietary Patterns And Their Effects On C-Reactive Protein Regarding Female Reproductive Disorders, Mckenzie Moe

Theses and Dissertations

Objectives: Chronic inflammation, as indicated by elevated C-reactive protein (CRP), plays a critical role in women’s reproductive health disorders. CRP is one of the most common biomarkers of inflammation. Dietary components, lifestyle factors, and women’s reproductive health disorders are major components that influence the levels of CRP within the body. Other influential factors include injury, infection, and illness. The purpose of this review is to explore the findings of literature available on nutrition interventions for CRP levels in consideration of women's reproductive disorders.

Methods: This narrative review examines the works of 15 articles, including 10 cross-sectional and five longitudinal designs. …

A Deep Learning Strategy To Identify Cell Types Across Species From High-Density Extracellular Recordings, Maxime Beau, David J Herzfeld, Francisco Naveros, Marie E Hemelt, Federico D'Agostino, Marlies Oostland, Alvaro Sánchez-López, Young Yoon Chung, Michael Maibach, Stephen Kyranakis, Hannah N Stabb, M Gabriela Martínez Lopera, Agoston Lajko, Marie Zedler, Shogo Ohmae, Nathan J Hall, Beverley A Clark, Dana Cohen, Stephen G Lisberger, Dimitar Kostadinov, Court Hull, Michael Häusser, Javier F Medina

Faculty, Staff and Students Publications

High-density probes allow electrophysiological recordings from many neurons simultaneously across entire brain circuits but fail to reveal cell type. Here, we develop a strategy to identify cell types from extracellular recordings in awake animals and reveal the computational roles of neurons with distinct functional, molecular, and anatomical properties. We combine optogenetics and pharmacology using the cerebellum as a testbed to generate a curated ground-truth library of electrophysiological properties for Purkinje cells, molecular layer interneurons, Golgi cells, and mossy fibers. We train a semi-supervised deep learning classifier that predicts cell types with greater than 95% accuracy based on the waveform, discharge …

Analyzing The Effectiveness Of Dance Movements In Enhancing Activities Of Daily Living For Geriatric Patients And Relevant Implications For Nursing Care, Rachel Ann Bruce

Student Scholar Showcase

Within the healthcare industry, the geriatric population holds a unique set of patient-care needs due to a myriad of physical and cognitive challenges. The presentation of these challenges can negatively impact the quality of a patient’s life and overall independence. One specific challenge providers must account for when providing care to geriatric patients is a decreased range of mobility as age increases. Reasons as to why mobility decreases varies, and include but are not limited to degenerative musculoskeletal conditions, poor nutrition, poor physical mobility or lack thereof, and other comorbidities.

According to peer-reviewed resources, by engaging in frequent Dance Movement …

The Effects Of Stress On Injury And Sickness Rates Of Middle-Distance And Distance D3 Athletes, Hailey D. Smith Ms.

Student Scholar Showcase

A runner intentionally stresses their body to experience an adaptation, however, issues occur when the body does not have time to recover. Male athletes (n = 14) on the University of Lynchburg’s distance and middle-distance team participated in this study. For 6 weeks, heart rate variability (HRV4training app), resting heart rate, perceived stress, and cortisol levels were assessed as indicators of recovery and stress. It was anticipated that indicators of physical stress such as higher resting heart rates and higher cortisol levels, would correspond with greater perceived stress and higher occurrence of injury and sickness. Cortisol (n = 2, p …

Vaginismus: Causes, Diagnosis, And Treatment Options, Heather Thomas

Best Integrated Writing

This paper provides a current review of the diagnosis, causes, and treatment outcomes for vaginismus. Of particular interest are predisposing factors, like a lack of sex education and a sexually restrictive upbringing, for the development of this disorder. Controversies surrounding US-based diagnostic criteria for female sexual dysfunction are also reviewed, and up-to-date and novel treatment options, like vaginal Botox injections, are described.

Pandemic Mythmaking: Covid-19 And Mythological Symbolism In Indian Folk Art, Ishani Anwesha Joshi, Sathyaraj Venkatesan

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Through a comprehensive analysis of Durga Bai Vyam’s “Samura Manthan” and Rupsona and Bahadur Chitrakar’s “Corona Rakshasa,” this article aims to investigate how mythological stories and themes have been used in the Indian context in folk art during the COVID-19 pandemic. The investigation will offer insights into how artists have leveraged mythological symbolism to infuse their works with richer layers of meaning, thereby engaging readers in a deeper exploration of the pandemic’s impact on society and the human psyche. By close-reading the aforementioned paintings, this study aims to illustrate the evolving visual representation of cultural narratives …

Investigating The Effects Of Palmitoylation In Ovcar3 Cells, Bethany Knopp, Emu Suzuki

Undergraduate Research Symposium 2025

Ovarian cancer is a disease characterized by the abnormal growth of cells in the ovaries often due to mutations in our DNA. Because ovarian cancer is typically diagnosed at advanced stages, the survival rate is less than 40% over a five-year period. One significant influence of this cancer is palmitoylation - a post- translational modification in which fatty acid chains are added to specific cysteine residues in proteins. This modification alters protein localization, stability, and function. When dysregulated, as seen in ovarian cancer, palmitoylation can promote irregular cell growth, mutations, and ultimately cancer. However, the role of palmitoylation in cancer …

Fourth Annual Research Symposium Program With Abstracts, Office Of Research Affairs, Boonshoft School Of Medicine

Boonshoft School of Medicine Research Symposium

The program booklet is a compilation of abstracts from oral and poster presentations at Wright State University's Fourth Annual Boonshoft School of Medicine Medical Research Symposium held on April 16, 2025.

A Qualitative Inquiry Of Exercise Preferences Among Hispanic Adults With Multiple Sclerosis, Stephanie L Silveira, Robert W Motl, Tania Ontiveros, Victoria Flores

Faculty, Staff and Student Publications

This study aimed to identify the components of an ideal exercise training program for Hispanics with multiple sclerosis (MS), cultural adaptations, and avenues for dissemination of information to this population. Online, semi-structured interviews were conducted with 27 adults with MS who identified as Hispanic. A deductive content analysis approach was applied for assessing necessary and desired components of an exercise program. Participants articulated preferences for "what," "when," "where," and "who" of exercise programs. All participants expressed an interest in aerobic training (n = 27) and need for resistance (n = 14), flexibility (n = 8), and neuromotor components (n = …

The Effect Of Female Athlete Menstrual Cycles On Their Athletic Performance, Millie Allgood

Honors Theses

Previous research studying the effects of the menstrual cycle on athletic performance has focused on women who do not participate in any athletic activity or participate at a recreational or professional level (Dupuit et al., 2023; Ekenros et al., 2022). Few studies have tested collegiate athletes and those which have examined theoretical effects or psychological impacts rather than direct performance outcomes (Carmichael et al., 2021). It is widely agreed upon that reproductive hormones have broad influences throughout the body including body composition, response to stress, appetite, energy expenditure, and blood-glucose regulation. These factors directly contribute to physiological abilities such as …

Short- And Long-Term Effects Of Early Versus Delayed Treatment With Ocrelizumab On Cerebellar Volume Loss In Patients With Rms And Ppms, Douglas L Arnold, Shannon Kolind, Haz-Edine Assemlal, Amit Bar-Or, Matilde Inglese, Ludwig Kappos, Katrin Parmar, Till Sprenger, Anthony Traboulsee, Irene M Vavasour, Jerry S Wolinsky, Corrado Bernasconi, Ulrike Bonati, Stefano Magon, Björn Tackenberg, Laura Gaetano

Faculty, Staff and Student Publications

Background: The cerebellum is a functionally and anatomically complex structure, which, in multiple sclerosis (MS), is affected by focal white/gray matter lesions and by secondary neurodegeneration of afferent/efferent connections to the supratentorial brain and the spinal cord.

Objectives: To assess the efficacy of ocrelizumab compared with interferon β-1a (IFN β-1a)/placebo on cerebellar volume loss and the effect of switching to ocrelizumab on volume change in the Phase III trials in relapsing MS (RMS, OPERA I/II) and in primary progressive MS (PPMS, ORATORIO).

Methods: Cerebellar volume change was computed using paired Jacobian integration and analyzed using a mixed-effect repeated measurement model. …

Exploring Clinician Understanding Of Therapeutic Alliance, Melissa Cole, Robert Welsh, Susan Shore

Internet Journal of Allied Health Sciences and Practice

Background. Therapeutic alliance is being discussed more readily in allied health literature regarding its potential to improve functional outcomes and patient satisfaction with care. The construct was imported from psychotherapy literature, and the degree and depth of familiarity among practicing physical therapists have not been fully explored. Purpose. This study aims to gain insight into the general level of familiarity and perceptions of therapeutic alliance among practicing clinicians. Methods. A survey was designed to capture a broad professional point of view and distributed using an anonymous link. Results. 73 surveys were completed. 43.3% of respondents reported they were not familiar …

Comparison Of Cognitive Functions, Sleep Quality, Emotional State, Balance And Physical Activity Levels Of University Students According To Academic Success, Sevim Oksuz, Ozde Depreli, Aytül Ozdil, Gozde Iyigun, Ender Angın, Betül Fatma Bilgin, Ayşen Karaman

Internet Journal of Allied Health Sciences and Practice

Purpose: This study was planned to compare the level of attention, dual-task abilities, sleep quality, emotional state (anxiety, depression, stress), dynamic balance, and physical activity level in low and high-academic success university students. Method: A total of 134 international undergraduate students between the age of 18 and 25 (low academic success group n=67, high academic success group n=67) were recruited for this study. The Trail Making Test (TMT) – A and B was used for attention assessment, the TMT While Walking – A and B was utilized for dual-task performance, the Pittsburgh Sleep Quality Index (PSQI) was utilized to assess …

Exploring The Relationship Between Preadmission Grade Point Average And Selected Occupational Therapy Program Outcomes, Robyn Wu, Jason Straub

The Open Journal of Occupational Therapy

Grade point averages (GPAs) are often considered in admissions to occupational therapy programs, but the predictive value of GPAs has not been well-studied. The current study investigated if preadmission GPAs predicted whether students passed all occupational therapy program coursework and the National Board for Certification in Occupational Therapy (NBCOT) exam on the first attempt. We analyzed 419 records of students who had matriculated in a master’s or doctoral occupational therapy program between 2010 and 2019. Binary logistic regression analyses were used to determine the effects of the independent variables science GPA and overall GPA on non-passing grades and on NBCOT …

Patient-Generated Graphs To Measure Pain And Fatigue In Persons With Neuralgic Amyotrophy, Renske Mj Janssen, Jos Ijspeert, Jan T. Groothuis, Nens Van Alfen, Alexander Ch Geurts, Maud Jl Graff, Tanya L. Packer, Edith Hc Cup

The Open Journal of Occupational Therapy

Background: Patients with neuralgic amyotrophy (NA) often experience limitations in daily activities because of pain and fatigue. Visual analogue graphs with a 24-hour x-axis can be used to rate pain and fatigue severity during a typical day. This study aimed to investigate the reliability and validity of the visual analogue graphs in patients with NA.

Method: Eight patients completed pain and fatigue graphs on three moments to examine inter-rater and test-retest reliability using Intraclass Correlation Coefficients (ICCs). Concurrent validity (n = 47) was tested by determining correlations between mean pain graph scores and numerical rating scale for pain (NRS-pain) and …

D.A.D.S. Club: Pilot Study Outcomes Of A 5-Week Parenting Program For Fathers In Recovery From Substance Use Disorder, Oaklee Rogers, Kristin Riordan, Elaina Corbin, Shannon Forman, Kara Grace Zahariades, Andrea Clemens, Keely Mcdowell

The Open Journal of Occupational Therapy

Background: Over 44 million adults have a substance use disorder, with approximately two million children living with a parent with this disorder. Approximately 40% to 60% of men who receive treatment have a child under 18 years of age. However, most parenting programs are developed and implemented for mothers with addiction, leaving fathers without this fundamental knowledge and skills.

Method: A pilot study using a longitudinal interventional study was conducted to explore the feasibility and effectiveness of an occupational therapy-developed program for fathers in recovery. Pre and post surveys, including the Parenting Sense of Competence, were collected from the …

The Effect Of Kefir Consumption On Gut And Mental Well-Being, Danh Nguyen, Kate Digges, Emerson Morris, Nicole Hinkley-Kellum, Cas Simpson, Dow Mccarty, Erik F. Y. Hom

Neuroscience Research Showcase

(All authors are affiliated with the Department of Biology: Center for Biodiversity and Conservation Research, University of Mississippi)

Members of the Hom Lab are studying the potential benefits of water kefir consumption on Irritable Bowel Syndrome (IBS) symptoms and mental health through Brain-Gut-Microbiota Axis modulation. Water kefir is a probiotic-rich beverage produced by the fermentation of sugar water using symbiotic aggregates of microbes known as water kefir grains (WKGs). WKGs consist of various yeast, lactic acid bacteria, and acetic bacteria bound together through extracellular polysaccharides. Probiotics are defined as foods or supplements that contain live, beneficial microbes that foster well-being …

Analyzing The Neuroprotective Effects Of Phytoestrogens And Aromatase On Male Zebra Finch Cerebellum Post Lesion, Tony Irovic, Grace Thompson, Lainy B. Day

Neuroscience Research Showcase

(All authors are affiliated with Interdisciplinary Studies: Neuroscience, Department of Biology, Interdisciplinary Neuroscience Minor, University of Mississippi)

Introduction:

• Estradiol (E2) & aromatase (AROM) are neuroprotective
• AROM enzymatically converts testosterone (T) into E2
• E2 alters testicular function and increases stroke and cancer risk
• Phytoestrogens, such as genistein (GEN), are plant derived compounds that have a similar chemical structure to E2 and some E2 -like effects
• GEN may possess the neuroprotective effects of E2 while not having the negative effects
• Zebra finch (ZF) brains are highly plastic, and the cerebellum (CB) has low AROM constitutively
• The CB has high levels of estrogen …

A Review Of Recent Studies On The Pathogenesis Of Systemic Sclerosis: Focus On Fibrosis Pathways, Sergio A. Jimenez, Fabian A. Mendoza, Sonsoles Piera-Velazquez

Scleroderma Center Faculty Papers

Systemic Sclerosis (SSc) is a systemic autoimmune disease of unknown etiology characterized by the development of frequently progressive cutaneous and internal organ fibrosis accompanied by severe vascular alterations. The pathogenesis of SSc is highly complex and, despite extensive investigation, has not been fully elucidated. Numerous studies have suggested that unknown etiologic factors cause multiple alterations in genetically receptive hosts, leading to SSc development and progression. These events may be functionally and pathologically interconnected and include: 1) Structural and functional microvascular and endothelial cell abnormalities; 2) Severe oxidative stress and high reactive oxygen species (3); Frequently progressive cutaneous and visceral fibrosis; …

Zonal Hepatocellular Responses To Acute Ethanol: Impacts On Mitochondrial Function And Metabolism, Matthew T. Savoca

MUSC Theses and Dissertations

Background: Ethanol (EtOH) consumption causes hepatocellular mitochondrial depolarization (mtDepo) and mitophagy. Here, the Aims were to develop techniques to characterize sublobular hepatocellular metabolism and the distribution of mtDepo and mitophagy after acute EtOH. Methods: Wildtype and GFP-LC3 transgenic mice were treated with EtOH or vehicle and administered the covalently labeling mitochondrial membrane potential (ΔΨ) reporter, MitoTracker Red (MTR), and/or the non-covalent ΔΨ reporter, rhodamine 123 (Rh123), prior to intravital imaging, liver fixation, or hepatocyte isolation. After in vivo MTR, hepatocytes were isolated and sorted. Enrichment in E-cadherin (ECAD) and cytochrome P4502E1 in periportal (PP) and pericentral (PC) hepatocytes, respectively, validated …

The Effects Of Cyclic Peptide [R4w4] In Combination With First-Line Therapy On The Survival Of Mycobacterium Avium, Melissa Kelley, Kayvan Sasaninia, Ali Badaoui, Ira Glassman, Arbi Abnousian, Nadia Rai, Rakesh K. Tiwari, Vishwanath Venketaraman

Pharmacy Faculty Articles and Research

Background: Mycobacterium avium (M. avium) is a nontuberculous mycobacterium (NTM) that can cause pulmonary and extrapulmonary infections mostly in immunocompromised individuals, such as those with HIV and diabetes. Traditionally, rifampicin (RIF) and azithromycin (AZ) have been used for a 12-month duration as first-line antibiotics against M. avium. Due to the increased multidrug resistance, novel ways, such as enhancement of macrophages response, are needed to provide adequate immune response required to clear M. avium infection.

Methods and findings: In this study, we aim to study the effects of using THP-1 cells, which are monocyte-like cells, to induce a …

G-Protein-Coupled Estrogen Receptor 1 And Pregnancy Confer Protection Against Hypertension In Older Females, Ravneet Singh, Supaporn Kulthinee, Victoria L Nasci, Elvis A Akwo, Chibundu Juliet Umunna, Rawan N Almutlaq, Oleh M Pochynyuk, Cassianne Robinson-Cohen, Eman Y Gohar

Faculty, Staff and Student Publications

No abstract provided.

Missense Mutation Of Msh6 Leucine 696 Has No Apparent Effect On The Dna Mismatch Repair Process, Razan H. Hammad, Rafia Rashid, Essence Tarrence, Christopher Bolden, Joanna E. Haye-Bertolozzi

XULAneXUS

Lynch Syndrome and Constitutional Mismatch Repair Deficiency are human diseases implicated in mutations of DNA mismatch repair (MMR) genes. This experiment tested a mutation of an MMR gene, MSH6, and evaluated how the mutation affected overall MMR effectiveness. Using the yeast Saccharomyces cerevisiae, we performed the CAN1 forward mutation assay to study msh6-L696F and its implications in the MMR process. We hypothesized that there would be a significant change in molecular function in the Msh6 protein in the presence of this mutation. Bioinformatic tools predicted that this amino acid change would have deleterious effects on MMR function. However, …

Nanopore Sequencing For The Detection And Preliminary Identification Of Viruses In Amblyomma Americanum L. (Acari: Ixodidae), Mary Katherine Humphrey, Lorenza Beati, Ella Zierdt, Saviour Dralabu

Honors College Theses

Hard-bodied ticks are known to carry a wide range of viruses, some of which can cause human diseases. In the U.S., the tick Amblyomma americanum is an important disease vector. The tick has recently expanded its distribution range and its abundance is rising in many areas of the U.S. Its virome is, however, largely underexplored. In this preliminary study, we used a Nanopore MinION sequencer to detect and identify the viral fauna in nymphal ticks (n=43) collected in Statesboro, GA. Extracted DNA and RNA were analyzed separately. Nucleic acids were pooled into 6 groups according to their collection dates and …

Volumetric Changes In Cerebellar Transverse Zones: Age And Sex Effects In Health And Neurological Disorders, Farshid Ghiyamihoor, Payam Paymani, Jarrad Perron, Azam Asemi-Rad, Mehdi Marzban, Aashka Mohite, Karen Ardila, Bara Aljada, Asghar Marzban, Mehnosh Toback, Sherif Eltonsy, Ji Hyun Ko, Tabrez J Siddiqui, Christopher J Steele, Jiming Kong, Mario Manto, M Ethan Macdonald, Jason S Gill, Roy V Sillitoe, Fuat Balcı, Iman Beheshti, Hassan Marzban

Duncan NRI Faculty and Staff Publications

Cerebellar volumetric changes are intricately linked to aging, with distinct patterns across its transverse zones, the functional subdivisions characterized by unique cytoarchitectural and connectivity profiles. Despite research efforts, the cerebellar aging process in health and neurological disorders remains poorly understood. In this study, we investigated the effects of age and sex on total cerebellum, transverse zone, and lobule volumes using MRI data from over 45,000 participants compiled from six neuroimaging datasets. We also propose a framework for estimating cerebellum age as an indicator of cerebellar health. Significant age‐dependent volume reductions were observed across transverse zones, with the …

The Placebo Effect For Opioid Abuse, Carly Longenecker

D.U.Quark

No abstract provided.

Perinatal Mood And Anxiety Disorders (Pmads): A Scoping Review, Christina Willis Bsn, Rn, Nia Wells Bsn, Rn, Miranda Smith Bsn, Rn, Kate Fouquier Cnm, Phd, Facnm

Doctor of Nursing Practice Projects

Purpose/Background Perinatal Mood and Anxiety Disorders (PMADs), including postpartum depression, anxiety, and psychosis, significantly impact maternal and infant health. This scoping review consolidates current literature on PMADs, emphasizing prevalence, risk factors, screening, and treatment. The primary aim is to identify research gaps and inform clinical guidelines to enhance perinatal care.

Methods A literature search followed PRISMA guidelines from August 2022 to November 2024. Databases searched included PubMed, CINAHL, Scopus, and Google Scholar, using terms such as "perinatal mood disorders" and "maternal mental health." Inclusion criteria were English-language studies from the past decade addressing PMADs during pregnancy and up to one …

Integrated Analysis Of Molecular And Clinical Features Associated With Overall Survival In Melanoma Patients With Brain Metastasis, Swaminathan Kumar, Meredith S Pelster, Merve Hasanov, Renato A Guerrieri, Courtney W Hudgens, Debora A Ledesma, Fuchenchu Wang, Grant M Fischer, Julie M Simon, Lauren E Haydu, Kalman V Katlowitz, Y N Vashisht Gopal, Jennifer L Mcquade, Lawrence N Kwong, Jason T Huse, Alexander J Lazar, Michael T Tetzlaff, Jeffrey E Gershenwald, Aron Y Joon, Ken Chen, Ziyi Li, Prahlad T Ram, Sherise D Ferguson, Michael A Davies

Faculty, Staff and Student Publications

Melanoma brain metastases (MBMs) are diagnosed in up to 60% of metastatic melanoma patients. Previous studies have identified clinical factors that correlate with overall survival (OS) after MBM diagnosis. However, molecular and immune features associated with OS are poorly understood. An improved understanding of the molecular and immune correlates of OS could provide insights into MBM patient outcomes and guide therapeutic development. Thus, we analyzed clinical features and outcomes of 74 melanoma patients who underwent surgical resection (via craniotomy) between 1991 and 2015 at our institution with RNA-seq data generated from their MBMs. The median post-operative OS was 8.6 months …

Single-Cell Analyses Reveal A Functionally Heterogeneous Exhausted Cd8+ T-Cell Subpopulation That Is Correlated With Response To Checkpoint Therapy In Melanoma, Kelly M Mahuron, Osmaan Shahid, Prachi Sao, Clinton Wu, Alexandra M Haugh, Laura A Huppert, Lauren S Levine, Margaret M Lowe, Michael Alvarado, Markee Micu, Katy K Tsai, Melissa Chow, Meromit Singer, Jason M Schenkel, Arlene H Sharpe, Michael D Rosenblum, Kristen E Pauken, Adil I Daud

Faculty, Staff and Student Publications

PD-1 pathway inhibitors have revolutionized cancer therapy. However, most patients do not durably benefit, highlighting the need for biomarkers to stratify patients as responders or nonresponders. Although CD8+ tumor-infiltrating lymphocytes (TIL) have been associated with immune checkpoint therapy response, there is no consensus on which CD8+ TIL subpopulations have the most prognostic value. Preclinical studies have focused on progenitor-like exhausted CD8+ T cells (TPEX) because TPEX proliferate more in response to PD-1 inhibitors than other exhausted T-cell (TEX) subpopulations. However, immune checkpoint inhibitor treatment drives TPEX differentiation into other TEX populations that can mediate antitumor immunity. These data complicate the …