Digital Commons Network^™

An Extracellular Protein Produced By Staphylococcus Hominis Strain Αm With Antimicrobial Activity Against Mycobacterium Spp, Sandra May Jacobsen

Biological Sciences Theses & Dissertations

The inhibitory activity exhibited by Staphylococcus hominis strain αM toward Mycobacterium species was examined. These studies included determining the conditions that maximized inhibitory agent production, analyzing characteristics of the agent, determining the time of agent production, ascertaining plasmid possession and the role of plasmids in the production of this inhibitory agent, and ascertaining the antimicrobial activity of other strains of S. hominis. On solid and in liquid media, S. hominis strain αM exhibits a unique inhibitory activity toward Mycobacterium species that is not demonstrated by Staphylococcus epidermidis or other S. hominis strains. Maximum agent production and recovery was …

An Exploratory Study Of The Correlates Of Drug Use Among Juveniles: Analysis Of Regional Variations In The United States, Denise D. Nation

Sociology & Criminal Justice Theses & Dissertations

Research on substance use and abuse has increased exponentially over the past twenty-five years. However, research focusing on regional differences in drug use seems to be very limited. The purpose of this study is to examine the correlates of drug use among juveniles in the four major geographic regions of the United States: the Northeast, the North Central, the South and the West. Here, drug use is defined in terms of alcohol and marijuana use for the age group 12-19. Four research hypotheses are proposed. The 1996 National Household Survey on Drug Abuse data was used to assess and analyze …

A Vibrio Cholerae Lysr Homolog, Aphb, Cooperates With Apha At The Tcpph Promoter To Activate Expression Of The Toxr Virulence Cascade, Gabriela Kovacikova, Karen Skorupski

Dartmouth Scholarship

We describe here a new member of the LysR family of transcriptional regulators, AphB, which is required for activation of the Vibrio cholerae ToxR virulence cascade. AphB activates the transcription of the tcpPH operon in response to environmental stimuli, and this process requires cooperation with a second protein, AphA. The expression of neither aphA or aphB is strongly regulated by environmental stimuli, raising the possibility that the activities of the proteins themselves may be influenced under various conditions. Strains of the El Tor biotype of V. cholerae typically exhibit lower expression of ToxR-regulated virulence genes in vitro than classical strains …

Dichotomy Between Naïve And Memory Cd4+ T Cell Responses To Fas Engagement, J. Desbarats, T. Wade, W. F. Wade, M. K. Newell

Dartmouth Scholarship

Engagement of Fas (APO-1, CD95), a member of the tumor necrosis factor receptor superfamily, can induce apoptotic cell death. However, Fas engagement also can costimulate lymphocyte proliferation. The physiologic regulation of these two outcomes is poorly understood. Here, we have used two systems, the first in vitro and the second in vivo, to demonstrate that naïve and memory CD4+ T cells display dichotomous responses to Fas ligation. Naïve CD4+ T cells (CD44lo, CD45RB+, CD62L+) die as a consequence of Fas ligation in the presence of anti-CD3 antibody, whereas memory T cells (CD44hi, CD45RB−, CD62L−), freshly isolated from the same …

Three V-Snares And Two T-Snares, Present In A Pentameric Cis-Snare Complex On Isolated Vacuoles, Are Essential For Homotypic Fusion, Christian Ungermann, Gabriele F. Von Mollard, Ole N. Jensen, Nathan Margolis, Tom H. Stevens, William Wickner

Dartmouth Scholarship

Vacuole SNAREs, including the t-SNAREs Vam3p and Vam7p and the v-SNARE Nyv1p, are found in a multisubunit "cis" complex on isolated organelles. We now identify the v-SNAREs Vti1p and Ykt6p by mass spectrometry as additional components of the immunoisolated vacuolar SNARE complex. Immunodepletion of detergent extracts with anti-Vti1p removes all the Ykt6p that is in a complex with Vam3p, immunodepletion with anti-Ykt6p removes all the Vti1p that is complexed with Vam3p, and immunodepletion with anti-Nyv1p removes all the Ykt6p in complex with other SNAREs, demonstrating that they are all together in the same cis multi-SNARE complex. After priming, which disassembles …

Ada News - 06/21/1999, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Interaction Between Fgf And Bmp Signaling Pathways Regulates Development Of Metanephric Mesenchyme., Andrew T. Dudley, R. E. Godin, E. J. Robertson

Journal Articles: Genetics, Cell Biology & Anatomy

Nephrogenesis in the mouse kidney begins at embryonic day 11 and ends approximately 10 days postpartum. During this period, new nephrons are continually being generated from a stem-cell population-the nephrogenic mesenchyme-in response to signals emanating from the tips of the branching ureter. Relatively little is known about the mechanism by which the nephrogenic mesenchyme cell population is maintained at the tips of the ureter in the presence of signals promoting tubulogenesis. Previous studies have shown that a loss of Bmp7 function leads to kidney defects that are a likely result of progressive loss of nephrogenic mesenchyme by apoptosis. The studies …

Ada News - 06/07/1999, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Characterizing Erythrocyte Motions In Flowing Blood, Markos Leggas

Theses and Dissertations (ETD)

Characterizing the probabilistic motions of cells is a prerequisite for the development of a general model of transport and surface deposition of white cells and platelets (WBC/P). These phenomena differ greatly when red blood cells (RBC) are present at normal levels, but follow convective diffusion in dilute suspensions. A critical need is to understand and characterize dispersion and diffusion as they apply to red cells in suspension flow. The dispersive motions of
0.5-micrometer beads and fluorescently labeled human RBC flowing in dilute (0.003%) and concentrated (25%) RBC suspensions, respectively, were characterized using fluorescence videomicroscopy methods, and times for individual tracer …

Presentation Of The Morris F Collen Award To Robert S Ledley, Dds, Naomi C. Broering

Library Staff Publications

No abstract provided.

Insertional Inactivation Of Treponema Denticola Tap1 Results In A Nonmotile Mutant With Elongated Flagellar Hooks, Ronald J. Limberger, Linda L. Slivienski, Jacques Izard, William A. Samsonoff

Department of Food Science and Technology: Faculty Publications

The treponemal fla operon is comprised of numerous motility-related genes; however, the initial gene of this operon, tap1, has no known function. A recently developed system to generate specific mutants in Treponema denticola was utilized to determine if Tap1 was essential for motility. T. denticola tap1 and flanking DNA were identified, cloned, and sequenced, and a suicide plasmid that contained tap1 interrupted with an erythromycin resistance cassette (ermF and ermAM) was constructed. Because of potential polar effects from this cassette, a second plasmid that contained tap1 interrupted with a modified erythromycin resistance cassette that lacked the putative ermF transcription …

Update - June 1999, Loma Linda University Center For Christian Bioethics

Update

In this issue:

-- February conference will examine future of faith-based health care
-- Stephen G. Post and the Debatable Demise of Dualism
-- A Tale of Two Physicians

Dentalgram - Vol. 13, No. 06, Loma Linda University School Of Dentistry

Dentalgram

In this issue:

• Graduation events
• More graduation events
• Awards for students and faculty
• Department news
• IFAC report
• IDP @ Yosemite

Oncolog, Volume 44, Number 06, June 1999, Jude Richard, Michael Courtney, Alison Ruffin, Dawn Chalaire

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• After Diagnosis, Another Hurdle: Cancer Screening for the Cancer Patient
• House Call: Reduce Cancer Risk With Regular Cancer Screening
• M. D. Anderson Participates in Multinational Breast Cancer Prevention Trial of Tamoxifen and Raloxifene
• Nerve Grafting Attempts to Restore Erectile Function After Prostatectomy
• M. D. Anderson Researchers Study Children's School Lunch Habits and Choices

Morphological Pattern And Frequency Of Thyroid Tumors, S H. Shah, S Muzaffar, I N. Soomro, Sheema H. Hasan

Department of Pathology and Laboratory Medicine

Objective:
The present study was done to evaluate the frequency of thyroid cancer and to find out the prevalence of histological types of thyroid tumor with respect to age and sex group.
Setting:
This study included consecutive cases of malignant tumors of thyroid gland, which were diagnosed in the Department of Pathology at the Aga Khan University Hospital, Karachi during the period of three years (1995-1997).
Methods:
These cases were evaluated on H & E stained sections from paraffin embedded 10% buffered formalin fixed tissue blocks. Special stains and immunohistochemical analysis were performed whenever required.Results: A total of 8541 malignant …

Pamela J. Stewart And Andrew Strathern's Review Of David Lipset's Curing And Healing: Medical Anthropology In Global Perspective, Pamela J. Stewart, Andrew Strathern

Pacific Studies

No abstract provided.

Euthanasia: Understanding Ethical Issues Through Role-Play, Setsuko Inoue

Critical and Creative Thinking Capstones Collection

Having transformed traditional ethics, people have empowered themselves and put ethics under their control. An individual's value has become the center of all decision making. where has ethics gone? Why has ethics been fossilized? when an individual desperately needs a litmus test to check his stance, why cannot he re-visit ethics and apply its insights to solving his problems? I wish to believe that there might be a legacy of conventional ethics in the form of universal rules, regardless of time, culture, and context, to be passed on to the next generation. Has God given us life, death, and choice …

Recidivism Among Jamaican Teenage Mothers : A Historical Cohort Study 1995-1998, Vonna Lou Caleb Drayton

Loma Linda University Electronic Theses, Dissertations & Projects

Pregnancy is the single most important reason why girls fail to complete secondary school in the Caribbean. The Women's Centre of Jamaica Foundation (WCJF) Program was established in 1978 to enable adolescent mothers 16 years and under to return to school, and to delay second pregnancies until completion of their education.

This historical cohort study assessed the effect of the WCJF Program on the incidence of repeat pregnancies over the period 1995-1998, and on the educational attainment of program participants. A random sample (n=260) of primiparous Jamaican adolescent mothers 12-16 years who gave birth in 1994 in the parishes of …

Effect Of Obturation And / Or Coronal Seal On The Success Of Root Canal Therapy : An In Vivo Histologic Investigation, Douglas Snider

Loma Linda University Electronic Theses, Dissertations & Projects

Numerous studies have examined coronal leakage with in vitro protocols. A search of the literature showed the absence of a long term in vivo, histologic study, examining the effect of coronal and/or radicular leakage on periradicular health.

The purpose of this study was to evaluate the effect of obturation and / or coronal seal in preventing the formation of periradicular lesions in dogs over time. The study was conducted on six beagle dogs. Premolars were cleaned and shaped. Sixteen roots in each dog were randomly assigned to one of four treatment groups of equal size. Group one was obturated using …

Antitumor Effect Of Il-2, P53, And Bax Naked Gene Transfer In C6 Glioma Cells, Peyman Haghighat

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract not included]

The Immunology Of Parturition, Ari Mayer Mackler

Loma Linda University Electronic Theses, Dissertations & Projects

Macrophage-derived factors have been implicated in the etiology of preterm labor but little is known about trafficking of this immune cell into the uterus or its regulation of uterine contractility in mice. Enhanced number and activation of macrophages was hypothesized to precede parturition. Studies characterized contractile activity and the distribution of macrophages in the uterus of C3/HeN mice before and after term (=day 19). Contractile activity by uterine strips on days 15 and 18 of pregnancy, the day of delivery, and 1 day postpartum indicate that an endogenous oscillator sustains high frequency contractions. Modest acceleration of the pacemaker occurred before …

Diagnosis And Treatment Of Swallowing Dysfunctions : Semg Cognitive Training As An Adjunct To Conventional Therapy, Vicky T. Nguyen

Loma Linda University Electronic Theses, Dissertations & Projects

This study compared surface electromyography (sEMG) cognitive training to conventional myofunctional therapy in controlling a swallowing dysfunction, commonly referred to as "tongue thrust". Nasal airway resistance (NAR) was assessed on every subject, to rule out mechanical obstruction that would prevent proper function. The scope of this investigation composed of evaluating baseline activity and dry swallowing patterns. Subjects were distributed to one of three treatment groups: sEMG cognitive training, conventional myofunctional therapy, and control, based on the degree of NAR (normal, mild, or moderate). Initial sEMG recordings were then taken on subjects that clinically demonstrated a tongue thrust habit to confirm …

Dna-Dna Checkerboard Hybridization To Identify Bacteria In Human Plaque Samples, Jasan Lee Zimmerman

Loma Linda University Electronic Theses, Dissertations & Projects

Previous research has shown that using DNA probes to identify the presence of specific bacteria in human plaque samples is superior to the commonly used anaerobic cultural procedures. Some periodontal pathogens are either uncultivable or very difficult to grow, such as Prevotella intermedia, P. nigrescens, and Eubacterium brachy. Digoxygenin probes were prepared for 15 oral bacteria. These probes were used with a checkerboard DNA-DNA hybridization scheme to analyze whether human plaque samples contained any of the 15 bacterial species. Our results have shown that this method is successful in identifying bacterial DNA present in the subgingival patient plaque …

Beta- And Gamma-Catenin Mutations, But Not E-Cadherin Inactivation, Underlie T-Cell Factor/Lymphoid Enhancer Factor Transcriptional Deregulation In Gastric And Pancreatic Cancer., K Caca, F T Kolligs, X Ji, M Hayes, J Qian, Alan M. Yahanda Md, Facs, D L Rimm, J Costa, E R Fearon

PCI Publications and Projects

Adenomatous polyposis coli (APC) mutations are present in >70% of colon cancers. The APC protein binds to beta-catenin (beta-cat), a protein first identified because of its role in E-cadherin (E-cad) cell adhesion. In some colon cancers lacking APC defects, mutations in presumptive glycogen synthase kinase 3beta phosphorylation sites near the beta-cat NH2 terminus appear to render beta-cat resistant to regulation by APC and glycogen synthase kinase 3beta. In cells with APC or beta-cat defects, beta-cat is stabilized and, in turn, binds to and activates T-cell factor (Tcf)/lymphoid enhancer factor (Lef) transcription factors. To further explore the role of APC, beta-cat, …

Interruption Of Multiple Cellular Processes In Ht-29 Epithelial Cells By Pseudomonas Aeruginosa Exoenzyme S, Joan C. Olson, Jennifer E. Fraylick, Eileen M. Mcguffie, Katherine M. Dolan, Timothy L. Yahr, Dara W. Frank, Timothy S. Vincent

Dartmouth Scholarship

Exoenzyme S (ExoS), an ADP-ribosylating enzyme produced by the opportunistic pathogen Pseudomonas aeruginosa, is directly translocated into eukaryotic cells by bacterial contact. Within the cell, ExoS ADP-ribosylates the cell signaling protein Ras and causes inhibition of DNA synthesis and alterations in cytoskeletal structure. To further understand the interrelationship of the different cellular effects of ExoS, functional analyses were performed on HT-29 epithelial cells after exposure to ExoS-producing P. aeruginosa 388 and the non-ExoS-producing strain 388DeltaS. Two different mechanisms of morphological alteration were identified: (i) a more-transient and less-severe cell rounding caused by the non-ExoS-producing strain 388DeltaS and (ii) a more-severe, …

1999 Commencement For College Of Health Professions

Jefferson College of Nursing Commencements

No abstract provided.

Data-Based Clinical Decision Making In The Treatment Of An Adolescent With Severe Conduct Problems, Douglass W. Nangle, Rebecca E. Carr, David J. Hansen

Department of Psychology: Faculty Publications

This case illustrates the contributions of continuous data monitoring to clinical decision making in the treatment of an adolescent with severe conduct problems. Treatment began with a comprehensive point system that required the continual monitoring of a range of target behaviors. Beginning each session with a review of graphs of the monitored behaviors helped keep the sessions focused and rewarded the client and his parents with visual feedback of their progress. A subjective rating system was also implemented in which the client’s parents quantified their evaluations of his overall behavior on a daily basis. This system enhanced the client’s parents’ …

Early Adolescence And Prosocial/Moral Behavior Ii: The Role Of Social And Contextual Influences, Gustavo Carlo, Richard A. Fabes, Deborah Laible, Kristina Kupanoff

Department of Psychology: Faculty Publications

This is the second installment of the special issue on prosocial and moral development in early adolescence. This issue focuses on social and contextual processes in young adolescents’ prosocial and moral behaviors. In this introductory article, a brief review of the research on parents or family, peers, school environment, culture, and nationality as correlates of prosocial and moral development was presented. The research indicates that the social context plays an important role in prosocial and moral development. However, research on the social and contextual correlates of prosocial and moral development in early adolescence is at an embryonic stage. Research is …

Study Of The Baig Gene In Bile Acid 7Α-Dehydroxylating Bacteria, Michael David

Masters Theses & Specialist Projects

Recently, intestinal bile acid 7α-dehydroxylating bacteria have been implicated in cholesterol gallstone disease. Eubacterium sp. strain V.P.I. 12708, a bile acid 7α-dehydroxylating bacteria, contains multiple bile acid inducible (bai) genes which are located on a large bai operon. Genes of this operon encode the enzymes responsible for bile acid 7α-dehydroxylation. The baiG gene encodes a bile acid transporter in Eubacterium sp. strain V.P.I. 12708. Utilizing the polymerase chain reaction I determined the presence or absence of baiG-like genes in six bile acid 7α-dehydroxylating strains: Clostridium sp. Strain TN-271, Eubacterium sp. strain 1-10, Clostridium bifermentans strain 1-55, Clostridium sordellii strain Y67, …

Santa Clara Magazine, Volume 41 Number 1, May 1999, Santa Clara University

Santa Clara Magazine

8 - A BRIDGE BETWEEN WORLDS By President PaulL. Locatelli, S.J. 'Go. President Locatelli examines the value of service learning for students, the University, and America's social fabric.

12 - A GIANT ARTIST By Ann Granning Bennett. Auguste Rodin's sculptures are now on view at the de Saisset Museum. The question is: How do high-profile art shows like this one come about?

18 - ARIZONA'S DIPLOMATIC TOP COP By Tamara Straus. Janet Napolitano ' 79 , Arizona's newly elected attorney general , discusses the challenges of public office as well as politics, ethics, and fighting crime.