Digital Commons Network^™

Sart, A Repressor Of Α-Hemolysin In Staphylococcus Aureus, Katherine A. Schmidt, Adhar C. Manna, Steven Gill, Ambrose L. Cheung

Dartmouth Scholarship

In searching the Staphylococcus aureus genome, we found several homologs to SarA. One of these genes, sarT, codes for a basic protein with 118 residues and a predicted molecular size of 16,096 Da. Northern blot analysis revealed that the expression of sarT was repressed by sarA and agr. An insertion sarT mutant generated in S. aureus RN6390 and 8325-4 backgrounds revealed minimal effect on the expression of sarR and sarA. The RNAIII level was notably increased in the sarT mutant, particularly in postexponential-phase cells, while the augmentative effect on RNAII was less. SarT repressed the expression of alpha-hemolysin, as determined …

Neuronal Cell Model And Methods Of Use Therefor, Craig S. Miller, Robert J. Danaher, Robert J. Jacob

Microbiology, Immunology and Molecular Genetics Faculty Patents

The present invention provides, inter alia, a PC12 cell quiescently infected with a neurotropic virus. Preferred quiescently-infected neural cells are those wherein the neurotropic virus is a neurotropic herpes virus. Methods related to these and other materials are also provided.

Celibacy, Sexual Exclusivity, And Illicit Drug Abstinence: Giving Up The Life As Taboo In Aids Prevention, Ibpp Editor

International Bulletin of Political Psychology

This article highlights social cognitions that seem to impede cost-effective approaches to AIDS prevention.

The Toxoplasma Gondii Protein Rop2 Mediates Host Organelle Association With The Parasitophorous Vacuole Membrane, Anthony P. Sinai, Keith A. Joiner

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Toxoplasma gondii replicates within a specialized vacuole surrounded by the parasitophorous vacuole membrane (PVM). The PVM forms intimate interactions with host mitochondria and endoplasmic reticulum (ER) in a process termed PVM–organelle association. In this study we identify a likely mediator of this process, the parasite protein ROP2. ROP2, which is localized to the PVM, is secreted from anterior organelles termed rhoptries during parasite invasion into host cells. The NH₂-terminal domain of ROP2 (ROP2hc) within the PVM is exposed to the host cell cytosol, and has characteristics of a mitochondrial targeting signal. In in vitro assays, ROP2hc is …

‘She’S 16 Years Old And There’S Boys Calling Over To The House’: An Exploratory Study Of Sexual Socialization In Latino Families, Marcela Raffaelli, Lenna L. Ontai

Department of Psychology: Faculty Publications

Compared to other ethnic groups in the USA, Latino populations are at high risk for negative sexual outcomes, including unplanned pregnancy and HIV/AIDS infection. The goal of this study was to explore the role of cultural beliefs and values in sexual socialization by focusing on the family socialization of adolescent romantic and sexual behavior described by 22 Latina/Hispanic women who took part in in-depth individual interviews. Four broad themes were explored: parental concerns regarding dating, family communication about sexual issues, family rules about dating, and actual dating and sexual experiences. Consistent with traditional cultural views, female romantic involvement outside of …

Guanylyl Cyclase C Agonists Regulate Progression Through The Cell Cycle Of Human Colon Carcinoma Cells., Giovanni Mario Pitari, M D Di Guglielmo, J Park, S Schulz, Scott A Waldman

Department of Pharmacology and Experimental Therapeutics Faculty Papers

The effects of Escherichia coli heat-stable enterotoxin (ST) and uroguanylin were examined on the proliferation of T84 and Caco2 human colon carcinoma cells that express guanylyl cyclase C (GC-C) and SW480 human colon carcinoma cells that do not express this receptor. ST or uroguanylin inhibited proliferation of T84 and Caco2 cells, but not SW480 cells, in a concentration-dependent fashion, assessed by quantifying cell number, cell protein, and [(3)H]thymidine incorporation into DNA. These agonists did not inhibit proliferation by induction of apoptosis, assessed by TUNEL (terminal deoxynucleotidyl transferase-mediated dNTP-biotin nick end labeling of DNA fragments) assay and DNA laddering, or necrosis, …

Teaching | Learning | Investigating | Discovering | Treating | Healing, Douglas J. Macmaster Jr., Paul C. Brucker

Thomas Jefferson University Administrative Documents and Reports

2001 Annual report of Thomas Jefferson University.

Ethics As Therapy: Philosophical Counseling And Psychological Health, Mike W. Martin

Philosophy Faculty Articles and Research

From the inception of philosophical counseling an attempt was made to distinguish it from (psychological) therapy by insisting that therapy could not be more misleading. It is true that philosophical counselors should not pretend to be able to heal major mental illness; nevertheless they do contribute to positive health—health understood as something more than the absence of mental disease. This thesis is developed by critiquing Lou Marinoff’s book, Plato not Prozac!, but also by ranging more widely in the literature on philosophical counseling. I also interpret philosophical counseling as a form of philosophical ethics.

Cyclooxygenase-2 And Prostaglandin E(2)(Pge(2)) Receptor Messenger Rnas Are Affected By Bovine Oocyte Maturation Time And Cumulus-Oocyte Complex Quality, And Pge(2) Induces Moderate Expansion Of The Bovine Cumulus In Vitro., M D Calder, A N Caveney, M E Westhusin, A J Watson

Obstetrics & Gynaecology Publications

Expression of cyclooxygenase-2 (COX-2) and prostaglandin E(2) (PGE(2)) receptor 2 (EP2) are necessary for rodent cumulus expansion in vivo. Prostaglandin E(2) receptor 3 (EP3) has been detected in bovine preovulatory follicles and corpora lutea. The current experiments examined the effect of PGE(2) on bovine cumulus expansion in vitro and expression of COX-2, EP1, EP2, EP3, and EP4 mRNAs in bovine cumulus-oocyte complexes (COCs) at 0, 6, 12, 18, and 24 h time points during maturation in vitro. Concentrations of PGE(2) above 50 ng/ml resulted in moderate cumulus expansion of bovine COCs, but expansion did not occur in the absence of …

Santa Clara Magazine, Volume 43 Number 1, Summer 2001, Santa Clara University

Santa Clara Magazine

2 - 150TH ANNIVERSARY MEMORIES In honor of SCU's sesquicentennial year, alumni reflect on their Bronco experiences.

4 - "SHEN FU" LEARNED THE GRACE OF ANGER By Tennant Wright, S.J. A friend shares his memories of Philip Oliger, S.J., who taught him that "passion is the sign that we are alive."

14 - WINGING IT By Adam Breen. Professor Bill Stover is a volunteer pilot for The Flying Doctors, a humanitarian group that brings free basic health and dental care to underprivileged communities in Mexico.

18 - SAVING GRACE By Elizabeth Kelly Gillogly '93. Santa Clara University's 150 years come …

Serotonin Transporter Promoter Polymorphism Genotype Is Associated With Behavioral Disinhibition And Negative Affect In Children Of Alcoholics, Geoffrey R. Twitchell, Gregory L. Hanna, Edwin H. Cook, Scott F. Stoltenberg, Hiram E. Fitzgerald, Robert A. Zucker

Department of Psychology: Faculty Publications

Background: Serotonergic (5-HT) dysfunction has been implicated in the etiology of both behavioral disinhibition (BD) and negative affect (NA). This work extends our previous finding of relationships between whole blood 5-HT and both BD and NA in pubescent, but not prepubescent, children of alcoholics and continues examination of a hypothesized role of 5-HT dysfunction in alcoholism risk. The long and short (L and S) variants of the 5-HT transporter gene-linked polymorphic region (5-HTTLPR) are responsible for differing transcriptional efficiencies in 5-HT uptake. Although associations have been found between the SS 5-HTTLPR genotype and severe alcoholism and neuroticism, recent reports describe …

The Kentucky Plan: An Innovative Approach To Professional Doctoral Education In Public Health., Joel M. Lee, F. Douglas Scutchfield

Preventive Medicine and Environmental Health Faculty Publications

The Doctor of Public Health (Dr.P.H.) degree is designed to prepare graduates for the highest levels of leadership in public health related careers in both public and private settings. The Kentucky School of Public Health has developed an innovative generalist professional degree program offering an extensive battery of courses based on the body of professional public health knowledge. The degree includes extensive supervised field experience as part of the academic training, linking theory with practice. Issues related to professional degrees, curriculum, accreditation and certification, admission, evaluation, plans for institutional cooperation, and demand are addressed.

Embryonic Stem Cell Research As An Ethical Issue: On The Emptiness Of Symbolic Value, Kevin Quinn

Faculty Publications

The ability to generate a wide variety of stem cell lines (in relatively renewable tissue cultures) opens up a whole new world of breathtaking possibilities for science and medicine. The possibilities include: "in vitro studies of normal embryo-genesis, human gene discovery, and drug and teratogen testing and as a renewable source of cells for tissue transplantation, cell replacement, and gene therapies." But it also opens up a world of complications.

Human EG and ES cells must be recovered from aborted fetuses or live embryos. Because primordial gonadal tissue is removed from fetuses after their death, the derivation of EO cells …

Interactions Among Murine Cytomegalovirus Us22 Family Gene Products That Influence Viral Pathogenesis, Zaruhi Karabekian

Theses and Dissertations in Biomedical Sciences

Cytomegalovirus (CMV) is a complex, ubiquitous herpesvirus that is characterized by acute, chronic, and latent infections. Monocytes-macrophages are the key target cell type involved in pathogenesis, which is most effectively studied using the murine model of CMV infection. Previously three murine CMV (MCMV) genes (M139, M140, and M141) were identified to regulate viral expression in cultured macrophages and in mice. These genes are members of the US22 gene family with respect to HCMV homology. There is no function assigned to the proteins encoded by these genes. However, deletion of M139, M140, and M141 significantly curtails growth of MCMV in macrophages …

A Longitudinal Study Examining The Stability Of Occupational Stressors Identified By Nursing Home Administrators, Gay Lynne Andrucci-Armstrong

Health Services Research Dissertations

As a result of the ever changing and expanding role of the nursing home administrator in conjunction with the stricter legislation governing nursing facilities over the past decade and the graying of America, a study specific to this population was warranted. The purpose of this study is to determine the relative change in the self-reported occupational stressors of nursing home administrators over a five year period (December 1994 to June 1999) secondary to the increased regulatory climate of the nursing home industry.

This study was carried out in three phases and included all practicing nursing home administrators in Virginia. Phase …

Positive Regulation Of Pka On Human Gonadotropin-Releasing Hormone (Hgnrh) Gene Expression In Human Placental Jeg-3 Cells, Zhaoyang Wen

Chemistry & Biochemistry Theses & Dissertations

Using the human placental choriocarcinoma JEG-3 cell line as an in vitro human placental model, we studied the mechanisms of the PKA positive regulation of the hGnRH gene expression in the human placenta. Studies in JEG3 cells showed that through the PKA catalytic subunit a, human GnRH upstream promoter activity was stimulated by PKA signaling pathway in a cAMP dependent mechanism. The sequence between —202 (Afl II) and —554 (BamH I) base pair in the human GnRH upstream promoter region appeared to be responsible for the PKA positive regulation of the gene expression. Furthermore, Western blot analysis demonstrated the involvement …

A Scanning Electron Microscopy Comparison Of The Effects Of The Arkansas Stone And The Ida-Hone Ceramic Stone On Curet Sharpness And Root Smoothness, Marian Gayle Rubino

Dental Hygiene Theses & Dissertations

Purpose: The purpose of this study was to determine if there are statistically significant differences between the Arkansas sharpening stone and the Ida-Hone ceramic sharpening stone in producing a sharper cutting edge on dental curets, and to determine if there is a statistically significant difference between the degree of root smoothness achieved by the sharpened curet cutting edges on extracted human teeth.

Methods: A two-group, randomized subject (n=39), double blind, post-test only design was employed using 39 factory new Hu-Friedy 11/12 gracey curets. Curets in group one were sharpened using the Ida-Hone ceramic stone, curets in group two were sharpened …

Parenting Behaviors Related To Young Adult Depressive Symptomology, Rebecca D. Abramson

Psychology Theses & Dissertations

Parental stress, hostility, warmth, acceptance, amount of control, and overall security of parent-child attachment appears to influence childhood and adult adjustment. Several relationships have been found between parenting style and the emotional and psychological well being of children and adolescents (e.g., Baumrind, 1967, 1971; Parker, 1979a, 1979b; Ge, Conger, Lorenz, & Simons, 1994; Ge, Best, Conger, & Simons, 1996). The present study examined the effects of perceived parenting styles on young adults' reported psychological distress and depressive symptomology. Participants viewed both mothers and fathers as equally attentive, supportive, and controlling, while viewing mothers as slightly more caring. However, participants' perceptions …

Estrogen Regulation Of The Pregnant Baboon Placental 11Β-Hydroxysteroid Dehydrogenase (11Β-Hsd)-Catalyzed Metabolism Of Cortisol And Cortisone And Its Effect On The Development Of The Fetal Hypothalamic-Pituitary-Adrenal Axis, William Adair Davies

Theses and Dissertations in Biomedical Sciences

Pepe and Albrecht previously demonstrated that the estrogen-regulated change in transuteroplacental metabolism of cortisol (F) and cortisone (E) from preferential reduction (E to F) at midgestation to oxidation (F to E) near term results in a decline of bioactive cortisol crossing the placenta and reaching the fetus culminating in activation of the hypothalamic-pituitary adrenal axis of the baboon and the ontogenesis of rate-limiting steroidogenic enzymes culminating in de novo F secretion. Protein kinase A (PK-A) activity in the baboon fetal adrenal gland cytosolic fraction was increased 2-fold both at term [day 165 of gestation (term = 184 days)] and following …

An Examinaton Of The Enhanced Primary Care Model For The Organization Of Team Care In Managing Type 2 Diabetes Mellitus In The Elderly Population, Carolyn Morcom Rutledge

Health Services Research Dissertations

The healthcare system is faced with overwhelming demands as a result of the growing elderly population, especially those with chronic illnesses. One disease that significantly impacts the morbidity and mortality of the elderly population, especially the minority and poor population, is type 2 diabetes. This population often lacks access to resources and quality healthcare that may be due to inadequate knowledge by the patients and their healthcare providers regarding available services. Current models of healthcare have not been effective in meeting the healthcare demands of this population. The purpose of this study was to evaluate the effectiveness of the Enhanced …

Tetrafluoroazidoaniline And Method Of Making And Using The Same, Hans Peter Spielmann, Kareem A.H. Chehade

Molecular and Cellular Biochemistry Faculty Patents

The invention is directed to a novel compounds comprising 4-azidotetrafluoroaniline and the alkyl, acyl and sulfonamide derivatives thereof and to methods of making and using the same. The novel compounds are useful as a photoaffinity probe to study protein structure and function. Two methods for preparing 4-azidotetrafluoroaniline are disclosed, each employing a stable carbamate intermediate from which the 4-azidotetrafluoroaniline is derived.

Crystal Structure Of The Sarr Protein From Staphylococcus Aureus, Yingfang Liu, Adhar Manna, Ronggui Li, Wesley E. Martin, Robert C. Murphy, Ambrose L. Cheung, Gongyi Zhang

Dartmouth Scholarship

The expression of virulence determinants in Staphylococcus aureus is controlled by global regulatory loci (e.g., sarA and agr). The sar (Staphylococcus accessory regulator) locus is composed of three overlapping transcripts (sarA P1, P3, and P2, transcripts initiated from the P1, P3, and P2 promoters, respectively), all encoding the 124-aa SarA protein. The level of SarA, the major regulatory protein, is partially controlled by the differential activation of the sarA promoters. We previously partially purified a 13.6-kDa protein, designated SarR, that binds to the sarA promoter region to down-modulate sarA transcription from the P1 promoter and subsequently SarA expression. SarR shares …

Ada News - 06/04/2001, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Multilamellar Bodies As Potential Scattering Particles In Human Age-Related Nuclear Cataracts, K. O. Gilliland, C. D. Freel, C. W. Lane, W. C. Fowler, M. J. Costello

Osteopathic Medicine, Jerry M. Wallace School of

No abstract provided.

Post-Infarction Left Venticular Free Wall Rupture, S M. Nadeem, S A. Sami, G Khan, N Basir, K A. Kazmi

Section of Cardiology

No abstract provided.

Adolescent And Young Adult Substance Use: Association With Sensation Seeking, Self Esteem And Retrospective Report Of Early Pubertal Onset. A Preliminary Examination, Catherine A. Martin, T. K. Logan, Carl Leukefeld, Rich Milich, Hatim Omar, Richard Clayton

Psychology Faculty Publications

Structured questionnaires were administered to investigate the relationship between early pubertal onset, substance abuse, sensation seeking, and self-esteem. The current study presents data from 1,002 subjects, who were followed from the 6th to the 10th grades and again at the age of 20. In females, early pubertal onset was associated with greater cigarette use and lower self-esteem. Further the interaction of early pubertal onset and low self-esteem was predictive for cigarette use in females, as was early pubertal onset and high sensation seeking. Late pubertal onset was associated with decreased alcohol use and lower sensation seeking in males, with the …

Commencement Program 2001, Loma Linda University

Commencement Programs

CONTENTS

2 | 2001 Events of Commencement

3 | The Academic Procession

4 | The Speakers

10 | The University Honorees

18 | The School Honorees

26 | The Programs

• School of Medicine, 27
• School of Dentistry, 34
• Graduate School, 42
• School of Nursing, 51
• School of Allied Health Professions - Physical Therapy, 56
• School of Allied Health Professions, 63
• School of Public Health, 71

Marek's Disease Virus (Mdv) Encodes An Interleukin-8 Homolog (Vil-8): Characterization Of The Vil-8 Protein And A Vil-8 Deletion Mutant Mdv, Mark S. Parcells, Su-Fang Lin, Robert L. Dienglewicz, Vladimir Majerciak, Dan R. Robinson, Hua-Chien Chen, Zining Wu, George R. Dubyak, Peter Brunovskis, Henry D. Hunt

Dartmouth Scholarship

Chemokines induce chemotaxis, cell migration, and inflammatory responses. We report the identification of an interleukin-8 (IL-8) homolog, termed vIL-8, encoded within the genome of Marek's disease virus (MDV). The 134-amino-acid vIL-8 shares closest homology to mammalian and avian IL-8, molecules representing the prototype CXC chemokine. The gene for vIL-8 consists of three exons which map to the BamHI-L fragment within the repeats flanking the unique long region of the MDV genome. A 0.7-kb transcript encoding vIL-8 was detected in an n-butyrate-treated, MDV-transformed T-lymphoblastoid cell line, MSB-1. This induction is essentially abolished by cycloheximide and herpesvirus DNA polymerase inhibitor phosphonoacetate, indicating …

Oncolog, Volume 46, Number 06, June 2001, Kathryn Hale, Dawn Chalaire

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Bladder Cancer Genetic Mapping Effort Builds on Human Genome Project
• Protocols: Clinical Trials for Bladder Cancer
• Keeping in Touch: Office of Physician Relations Builds Partnerships, Maintains Contact with Community Physicians
• House Call: Overcoming Barriers to Cancer Screening for Men

Enhanced Detection Of Surface-Associated Bacteria In Indoor Environments By Quantitative Pcr, Mark P. Buttner, Patrica Cruz-Perez, Linda Stetzenbach

Environmental & Global Health Faculty Research

Methods for detecting microorganisms on surfaces are needed to locate biocontamination sources and to relate surface and airborne concentrations. Research was conducted in an experimental room to evaluate surface sampling methods and quantitative PCR (QPCR) for enhanced detection of a target biocontaminant present on flooring materials. QPCR and culture analyses were used to quantitateBacillus subtilis (Bacillus globigii) endospores on vinyl tile, commercial carpet, and new and soiled residential carpet with samples obtained by four surface sampling methods: a swab kit, a sponge swipe, a cotton swab, and a bulk method. The initial data showed that greater overall sensitivity was obtained …