Digital Commons Network^™

The Impact Of Performance Feedback On Handwashing Behaviors., Vera Benita Brock

All ETDs from UAB

No abstract provided.

Health Promotion Behaviors Of Rural Women With Heart Failure., Carolyn S. Pierce

All ETDs from UAB

No abstract provided.

Creation Of Three Nutritional Mixes Based On Amaranth (Amaranthus Caudatus) And Other Andean Crops For School-Aged Children (Cochabamba-Bolivia), Sandra Carola Delgadillo Barriga

Theses and Dissertations

In Bolivia we have three very valuable Andean grains: amaranth (Amaranthus caudatus), quinoa (Chenopodium quinua), and canihua (Chenopodium pallidicaule), which combined with cereals and legumes can become a complete and balanced food in order to combat the malnutrition that is so widespread in our region. In this study, various nutritional mixes were formulated, based on amaranth and other Andean crops, and the three best were chosen according to nutrition, palatability, and cost. They were: Andean Baby Food, Mixicereal, and Amaranth Cookie, which have an amino acid complement between 79% and 100% and give satisfactory amounts of protein, energy, and minerals …

Clinical Significance Of Molecular Expression Profiles Of Hürthle Cell Tumors Of The Thyroid Gland Analyzed Via Tissue Microarrays, A. Hoos, A. Stojadinovic, B. Singh, M. Dudas, Denis H. Y. Leung, A. Shaha

Research Collection School Of Economics

Hürthle cell tumors are rare thyroid neoplasms for which disease biology is poorly understood and diagnosis of carcinoma can be challenging. The aim of the study was to characterize molecular expression profiles of Hürthle cell tumors and to determine the clinical significance of identified phenotypes. Paraffin-embedded tissue cores of normal thyroid (n = 18), and histopathologically well-defined Hürthle cell adenomas (n = 27), Hürthle cell tumors of unknown malignant behavior (n = 7), and minimally (n = 14) and widely (n = 21) invasive Hürthle cell carcinomas were arrayed in triplicate on tissue microarrays. Expression profiles of p53, mdm-2, p21, …

Disease Management And Liability In The Human Genome Era, Larry I. Palmer

Faculty Publications

No abstract provided.

Utility Of Pender's Model In Describing Health -Promoting Behaviors In Thai Women With Systemic Lupus Erythematosus., Aurawamon Sriyuktasuth

All ETDs from UAB

No abstract provided.

Intrafamilial Phenotypic Variability In Friedreich Ataxia Associated With A G130v Mutation In The Frda Gene, Dominick Mccabe, Nicholas Wood, Fergus Ryan, Michael Hanna, Sean Connolly, David Moore, Janice Redmond, David Barton, Raymond Murphy

Articles

Background: Most patients with Friedreich ataxia (FA) have a GAA trinucleotide repeat expansion in intron 1 of the FA gene (FRDA) on both arms of chromosome 9. However, some patients are compound heterozygotes and harbor a GAA expansion on one allele and a point mutation on the other. Compound heterozygous patients with FA who have a GAA expansion and a G130V mutation have been reported to have an atypical phenotype with a slow disease progression, minimal or no ataxia, or gait spasticity. Objective: To describe intrafamilial phenotypic variability in a GAA expansion/G130V mutation compound heterozygous family with FA. Setting: Tertiary …

Acute Myeloid Leukemia In Children In Pakistan: An Audit, S. Zaki, I. A. Burney, Mohammad Khurshid

Section of Haematology/Oncology

Objective: To see the clinical features and treatment of children with Acute Myeloid Leukemia (AML) in Pakistan. Setting: Tertiary referral at a specialist Hematology/Oncology center.
Methods: Retrospective, chart-based review of children (less than 14 years) admitted to the hospital with a diagnosis of AML between January 1987 and August 1997.
Results: A total of 23 patients were admitted. There were 18 males and 5 females. The mean age was 8 5 years. M3 was the commonest morphological subtype (43%). Twenty- two percent of the patients presented with hyperleucocytosis (TLC> 100 x i09 /L) and 95% with an elevated …

Predominant Nursing Students' Learning Profile And Nursing Faculty Teaching Styles As Related To Final Course Grade In A Baccalaureate Nursing Program In Puerto Rico, Lourdes Méndez Cruz

Dissertations

Problem. The purpose o f this study was to investigate the relationship between students’ predominant learning profile, faculty teaching preferences, and the final course grade in a 4-year nursing program at a private Christian university in Puerto Rico.

Method. A non-experimental descriptive research design was used for this study. The Learning Profile Indicator was administered to all nursing students («=138) while the Teaching Style Inventory was given to all faculty («=9). Demographic sheets were also given to both students and faculty Students’ final course grades were obtained from the faculty. Descriptive (frequency, means and standard deviations) and inferential statistics …

A Study On The Determination Of Some Parameters Of The Scorpion Fish (Scorpaena Porcus Linne., 1758) Caught By Bottom Nets In The Area Of Sinop In Terms Of Fishery Biology, Habi̇l Uğur Koca

Turkish Journal of Veterinary & Animal Sciences

In the present research, which was carried out during the period of June 1996 to May 1997 in order to determine some population parameters of the scorpion fish (Scorpaena porcus Linne., 1758) in the Sinop region, a total of 633 individuals were investigated according to age, length, weight and sex composition, growth rate, the relationship of length-weight, Von Bertalanffy Growth Formula and the condition factor. The sex ratio of the stock was determined to be 43.76% female and 56.24% male and it was found that the fish were 1-6 years old. In the research, the relationship of length-weight was found …

Acridine Orange For Malaria Diagnosis: Its Diagnostic Performance, Its Promotion And Implementation In Tanzania, And The Implications For Malaria Control, J. Keiser, J. Utzinger, Zul Premji, Y. Yamagata, B.H. Singer

Pathology, East Africa

One hundred years ago, Giemsa's stain was employed for the first time for malaria diagnosis. Giemsa staining continues to be the method of choice in most malarious countries, although, in the recent past, several alternatives have been developed that exhibit some advantages. Considerable progress has been made with fluorescent dyes, particularly with Acridine Orange (AO). The literature on the discovery, development and validation of the AO method for malaria diagnosis is reviewed here. Compared with conventional Giemsa staining, AO shows a good diagnostic performance, with sensitivities of 81.3%-100% and specificities of 86.4%-100%. However, sensitivities decrease with lower parasite densities, and …

In-Gel Purified Ditags Direct Synthesis Of Highly Efficient Sage Libraries, Saroj P. Mathupala, Andrew E. Sloan

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

SAGE (serial analysis of gene expression) is a recently developed technique for systematic analysis of eukaryotic transcriptomes. The most critical step in the SAGE method is large scale amplification of ditags which are then are concatemerized for the construction of representative SAGE libraries. Here, we report a protocol for purifying these ditags via an 'in situ' PAGE purification method. This generates ditags free of linker contaminations, making library construction simpler and more efficient.

Results

Ditags used to generate SAGE libraries were demarcated 'in situ' on preparative polyacrylamide gels using XC and BPB dyes, which precisely straddle the ditag …

How Physicians Feel About Assisting Female Victims Of Intimate-Partner Violence, Ramani N. Garimella, Stacey B. Plichta, Clare Houseman, Laurel Garzon

Community & Environmental Health Faculty Publications

Purpose. To assess the feelings of physicians about assisting female victims of intimate-partner violence (IPV), and to examine factors related to positive and negative feelings about assisting victims of IPV.

Method. In 1998, a total site sample of 150 physicians practicing in a large general hospital in the area of Virginia Beach, Virginia, was surveyed by questionnaire via the mail. Four specialties were represented: emergency medicine, family practice, obstetrics-gynecology, and psychiatry. The questionnaire asked about medical training and training in assisting victims of IPV The physicians' feelings about working with victims of IPV were measured on a nine-item, five-point …

The Effects Of An Aquatic Intervention Program On The Discomforts Of Pregnancy, Sheila Anne O'Hanlon Smith

Theses and Dissertations

Approximately 50% to 80% of pregnant women complain of low back pain, edema, and other discomforts. Although exercise programs have been recommended to decrease the discomforts of pregnancy and improve body image. There is a paucity of research in this area. There is very little research on exercise in water, despite theoretical and empirical advantages of such activity.

This study’s aims were to determine the impact of an aquatic exercise intervention program on pregnant women’s body image, self-efficacy, perception of barriers to health-promoting behaviors, health-promoting behaviors, mobility, and discomforts. Pender’s Health Promotion Model (1996) served as the framework for the …

Working Length Determination In Palatal Roots Of Maxillary Molars, Melanie A. Kim-Park

Theses and Dissertations

The aim of this study was to determine if a buccal curvature in the palatal roots of maxillary molars affected the clinician’s ability to accurately determine working length. Twenty-seven extracted, human maxillary molars were sorted by palatal root curvatures as J- and C-type and the angle of curvature was determined. Straight-line access was made and a #20 file was placed into the canal until the tip was visible at the apical foramen then withdrawn. The file, tooth and calibration wire were radiographed on one image using the RVG. Actual (file) and radiographic (tooth) lengths were determined using the RVG ruler. …

Faculty Publications 2001, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 706 publications.

Initiation Of Apoptosis In Cells Exposed To Medium From The Progeny Of Irradiated Cells: A Possible Mechanism For "Bystander" Induced Genomic Instability., Fiona Lyng, B. Maguire, B. Mcclean, C. Seymour, Carmel Mothersill

Articles

Much evidence now exists regarding radiation-induced bystander effects, but the mechanisms involved in the transduction of the signal are still unclear. The mitogen-activated protein kinase (MAPK) pathways have been linked to growth factor-mediated regulation of cellular events such as proliferation, senescence, differentiation and apoptosis. Activation of multiple MAPK pathways such as the ERK, JNK and p38 pathways have been shown to occur after exposure of cells to radiation and a variety of other toxic stresses. Previous studies have shown oxidative stress and calcium signaling to be important in radiation-induced bystander effects. The aim of the present study was to investigate …

Mutation Detection For Genotype/Phenotype Correlation Studies In Autosomal Dominant Polycystic Kidney Disease, Marie Mccluskey

Theses: Doctorates and Masters

Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common genetic disorders (Gabow, 1993), with an estimated prevalence of 1 in 400 to 1 in 1000 (Dalgaard, 1957; Gabow, 1993). The disease is the fourth leading cause of renal failure with more than 10 million people affected worldwide (Grantham, 1997). Mutations in at least two genes (PKD1, PKD2) can lead to the disease (European PDK Consortium, 1994; Mochizuki, et al, 1996). This project deals with autosomal dominant PKD, caused by mutations in the PKD1 gene, which account for 85% of reported cases.

Fatigue, Ambulance Perspectives In A Comparative Study Between Air And Road Transports, Wayne J. Mckenna

Theses: Doctorates and Masters

The study of fatigue in the health and transport sectors has predominantly been focused on night shift or day shift operations. This study has been applied to the hours of operation of Ambulance Officers who perform both night and day shifts, while also being required for on-call. The Officer on-call is on stand-by between shifts, to be available to respond for duties from the completion of one shift until the commencement of the next, usually a ten-hour shift. Studies of a similar focus have not been identified so a comparison of these specific findings has not been possible. However trends …

Placental Peroxisome Proliferator-Activated Receptor Gamma And Leptin Receptor In Human Pregnancy, Gwyneth H. Gladstones

Theses : Honours

Leptin is a 16kDa peptide hormone that has recently been implicated in human reproductive processes. Like other hormones, leptin exerts its influence through its receptor. Of the three major isoforms of the receptor (OB-Ra, OB-Rb, and 0B-Re), OB-Ra (the dominant short form) has been implicated in transport processes within rat and human placentas, suggesting a role for it in implantation and pregnancy maintenance. The peroxisome proliferator-activated receptors (PPARs) are ligand-dependent transcription factors that are a subfamily of nuclear hormone receptors. PPARs form heterodimers with another nuclear hormone receptor, RXRα, before binding to PPAR response elements (PPREs). Recently PPARϒ/RXRα heterodimers have …

Transcriptional Interference By Independently Regulated Genes Occurs In Any Relative Arrangement Of The Genes And Is Influenced By Chromosomal Integration Position, Susan K. Eszterhas, Eric E. Bouhassira, David I. K. Martin, Steven Fiering

Dartmouth Scholarship

Transcriptional interference is the influence, generally suppressive, of one active transcriptional unit on another unit linked in cis. Its wide occurrence in experimental systems suggests that it may also influence transcription in many loci, but little is known about its precise nature or underlying mechanisms. Here we report a study of the interaction of two nearly identical transcription units juxtaposed in various arrangements. Each reporter gene in the constructs has its own promoter and enhancer and a strong polyadenylation signal. We used recombinase-mediated cassette exchange (RMCE) to insert the constructs into previously tagged genomic sites in cultured cells. This …

A Qualitative Analysis For Sex Determination In Humans Utilizing Posterior And Medial Aspects Of The Distal Humerus, Veronica L. Wanek

Dissertations and Theses

Visual and metric analysis both provide accepted methods for sex determination in humans. Visual ascertainment uses differing morphological traits in males and females to establish sex. Researchers have continually sought accurate methods of sexing long bones when skulls or pelves are absent or fragmented. These long bone elements may not have sexually distinct characteristics, but tend to survive in the field quite well.

Metric analysis depends on size dimorphism between males and females to correctly assign sex. Metric methods fail where the sexes overlap or when skeletal elements cannot be assigned to their correct biological population. Under these conditions, visual …

Bottle-Feeding Mothers' Experiences Of Coping In A Pro-Breastfeeding Context : The Case For A Caring-Options-Responsive Model Of Midwifery Services, Elizabeth P. Duffy

Theses: Doctorates and Masters

The original contribution of this qualitative study is that it sketches the front-line of the contested domain of infant feeding choices by exploring the under-researched experiences and perceptions of mothers who actively choose to bottle-feed their babies. Twelve bottle-feeding mothers in Western Australia participated in open-ended, in-depth interviews. The interview findings were further explored using participant observations of relevant hospital practices and critical, hermeneutic re-readings of midwifery's professional and policy documents. The thesis argues that bottle-feeding is marginalised by the midwifery profession that currently defines its practices in accordance with policies such as the Baby Friendly Hospital Initiative. Bottle-feeding mothers …

Evaluation Of Feeding Chlamydospores Of Duddingtonia Flagrans To Ewe/Lamb Pairs And Weaned Lambs To Biologically Control Levels Of Haemonchus On Pasture, Natalee Peart

LSU Master's Theses

Gastroenteritis caused by the nematode parasite Haemonchus contortus is a serious concern for small ruminants in the tropics and subtropics. Control is traditionally by anthelmintics and pasture management, however this specific nematode parasite has become resistant to many anthelmintics and research is now focused on novel control methods including biological control using the nematode-trapping fungus, Duddingtonia flagrans. The objective of this study was to investigate the effectiveness of feeding chlamydospores of the fungus at a dosage of 5 x 105 spores/kg of BW to ewe/lamb pairs (Trial 1) and weaned lambs (Trial 2) for a total of 17 and 8 …

Introduction To Written Symposium On Public Health And International Law, David P. Fidler

Articles by Maurer Faculty

No abstract provided.

Approaches To Measuring The Frequency Of Achondroplasia And Hypochondroplasia Causing Fgfr-3 Mutations In Human Sperm, Andrew Timothy Daters

LSU Master's Theses

Achondroplasia and hypochondroplasia are two forms of skeletal dysplasias caused predominantly by single base mutations in the fibroblast growth factor receptor 3 gene (FGFR-3). The mutation for achondroplasia is a G1138A/C substitution and the mutation for hypochondroplasia (occurring about 50% of the time) is a C1620A/G substitution. Recent genetic studies have shown that spontaneous mutations for achondroplasia and hypochondroplasia occur exclusively on the paternally derived chromosome, suggesting that these mutations occur preferentially during spermatogenesis. For unknown reasons, the mutation rates at these FGFR-3 nucleotides appear to occur at a much higher frequency than nucleotide specific mutation rates observed in other …

Staphylococcus Aureus Agr And Sara Functions Are Required For Invasive Infection But Not Inflammatory Responses In The Lung, Geoffrey Heyer, Shahryar Saba, Robert Adamo, William Rush, Grace Soong, Ambrose Cheung, Alice Prince

Dartmouth Scholarship

Staphylococcus aureus strains lacking agr- and sarA-dependent gene products or specific MSCRAMM (microbial surface components recognizing adhesive matrix molecules) adhesins were compared for the ability to activate inflammatory responses in the lung. The mutants were evaluated for virulence in a mouse model of pneumonia and by quantifying their ability to stimulate interleukin-8 (IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF) expression in respiratory epithelial cells. In a neonatal mouse, only strains with intact agr and sarA loci were consistently associated with invasive, fatal pulmonary infection (P < 0.001) and sarA was specifically required to cause bacteremia (P < 0.001). The agr and/or sarA mutants were, nonetheless, fully capable of producing pneumonia and were as proficient as the wild-type strain in stimulating epithelial IL-8 expression, a polymorphonuclear leukocyte chemokine, in airway cells. In contrast, agr and especially sarA mutants induced less epithelial GM-CSF expression, and MSCRAMM mutants lacking fibronectin binding proteins or clumping factor A, a ligand for fibrinogen, were unable to stimulate epithelial GM-CSF production. The ability to induce IL-8 expression was independent of the adherence properties of intact bacteria, indicating that shed and/or secreted bacterial components activate epithelial responses. While conserved staphylococcal components such as peptidoglycan are sufficient to evoke inflammation and cause pneumonia, the agr and sarA loci of S. aureus are critical for the coordination of invasive infection of the lungs.

'Bringing You Up To Speed' Challenges Of A Mother Using Amphetamines : A Case Study, Derek Bilton

Theses : Honours

The purpose of the current study was to explore the challenges of parenting from the perspective of a woman who uses amphetamines. The types of parenting challenge that arise as a direct result of both intoxication and a drug using lifestyle were examined. The study also investigated the types of coping strategies that the participant adopted in meeting her parenting challenges in conjunction with the factors that aided and impeded her preferred method of responding. The study extends onto the existing literature by drawing a participant from a non-clinical setting. The participant was recruited from word of mouth field recruitment. …

Rearing The Child Who Is Technology Dependent: Perceptions Of Parents And Home Care Nurses, Maureen O'Brien, Carole B. Wegner

College of Nursing Faculty Research and Publications

ISSUES AND PURPOSE. Most children who are dependent on technology for survival live with their families at home. This study explores the perceptions of parents and home care nurses regarding rearing the technology-dependent child.

DESIGN AND METHODS. In this qualitative study, interviews were conducted with 16 parents whose child is technology dependent and 15 registered nurses who provided home care.

RESULTS. Rearing the child who is technology dependent is similar to but different from raising other children. Parental communication and negotiation of child-rearing expectations with home care nurses is essential.

PRACTICE IMPLICATIONS. Improved collaboration and communication between parents and nurses …

Genes Encoding Several Poly (Adp-Ribose) Glycohydrolase (Parg) Enzymes, The Proteins And Fragments Thereof, And Antibodies Immnoreactive Therewith, Myron Jacobson, Elaine L. Jacobson, Jean-Christoph Amé, Winston Lin

Graduate Center for Nutritional Sciences Faculty Patents

The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods …