Digital Commons Network^™

Defective Cd19-Dependent Signaling In B-1a And B-1b B Lymphocyte Subpopulations., Goutam Sen, Hsin-Jung Wu, Gabriel Bikah, Chandrasekar Venkataraman, Darrell A Robertson, E Charles Snow, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Peritoneal and pleural cavities in mice and humans contain a unique population of B-lymphocytes called B-1 cells that are defective in B cell antigen receptor (BCR) signaling but have an increased propensity to produce autoantibodies. Several molecules such as Btk, Vav, and CD19 known to be important for BCR signaling have been shown to be critical for the development of B-1 cells from undefined precursors. Here we demonstrate that B-1 cell unresponsiveness to BCR cross-linking is in part due to defective signaling through CD19, a molecule known to modulate signaling thresholds in B cells. The defective CD19 signaling is manifested …

Histiocytosis X Of The Vulva, R M. Rizvi, C Nasreen, N Jafri

Department of Obstetrics & Gynaecology

No abstract provided.

Mycobacterium Smegmatis L-Alanine Dehydrogenase (Ald) Is Required For Proficient Utilization Of Alanine As A Sole Nitrogen Source And Sustained Anaerobic Growth, Zhengyu Feng, Nancy E. Caceres, Gautam Sarath, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

NAD(H)-dependent L-alanine dehydrogenase (EC 1.4.1.1) (Ald) catalyzes the oxidative deamination of L-alanine and the reductive amination of pyruvate. To assess the physiological role of Ald in Mycobacterium smegmatis, we cloned the ald gene, identified its promoter, determined the protein expression levels, and analyzed the combined effects of nutrient supplementation, oxygen availability, and growth stage on enzyme activity. High Ald activities were observed in cells grown in the presence of L- or D-alanine regardless of the oxygen availability and growth stage. In exponentially growing cells under aerobic conditions, supplementation with alanine resulted in a 25- to 50-fold increase in the …

Mycobacterium Smegmatis L-Alanine Dehydrogenase (Ald) Is Required For Proficient Utilization Of Alanine As A Sole Nitrogen Source And Sustained Anaerobic Growth, Zhengyu Feng, Nancy E. Caceres, Gautam Sarath, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

NAD(H)-dependent L-alanine dehydrogenase (EC 1.4.1.1) (Ald) catalyzes the oxidative deamination of L-alanine and the reductive amination of pyruvate. To assess the physiological role of Ald in Mycobacterium smegmatis, we cloned the ald gene, identified its promoter, determined the protein expression levels, and analyzed the combined effects of nutrient supplementation, oxygen availability, and growth stage on enzyme activity. High Ald activities were observed in cells grown in the presence of L- or D-alanine regardless of the oxygen availability and growth stage. In exponentially growing cells under aerobic conditions, supplementation with alanine resulted in a 25- to 50-fold increase in the …

Clinical And Epidemiological Correlates Of Genotypes Within The Mycobacterium Avium Complex Defined By Restriction And Sequence Analysis Of Hsp65, Sandra C. Smole, Fionnuala Mcaleese, Jutamas Ngampasutadol, C. Fordham Von Reyn, Robert D. Arbeit

Dartmouth Scholarship

Species identification of isolates of the Mycobacterium avium complex (MAC) remains a difficult task. Although M. avium and Mycobacterium intracellulare can be identified with expensive, commercially available probes, many MAC isolates remain unresolved, including those representing Mycobacterium lentiflavum as well as other potentially undefined species. PCR restriction analysis (PRA) of the hsp65 gene has been proposed as a rapid and inexpensive approach. We applied PRA to 278 MAC isolates, including 126 from blood of human immunodeficiency virus (HIV)-infected patients, 59 from sputum of HIV-negative patients with chronic obstructive pulmonary disease, 88 from environmental sources, and 5 pulmonary isolates from …

Coupling Of Termination, 3′ Processing, And Mrna Export, C. M. Hammell, Stefan Gross, Daniel Zenklusen, Catherine V. Heath, Francoise Stutz, Claire Moore, C. N. Cole

Dartmouth Scholarship

In a screen to identify genes required for mRNA export in Saccharomyces cerevisiae, we isolated an allele of poly(A) polymerase (PAP1) and novel alleles encoding several other 3′ processing factors. Many newly isolated and some previously described mutants (rna14-48, rna14-49, rna14-64, rna15-58, and pcf11-1 strains) are defective in polymerase II (Pol II) termination but, interestingly, retain the ability to polyadenylate these improperly processed transcripts at the nonpermissive temperature. Deletion of the cis-acting sequences required to couple 3′ processing and termination also …

5 Questions–And Answers–About Negative Pressure Wound Therapy, Luther C. Kloth

Physical Therapy Faculty Research and Publications

No abstract provided.

Ada News - 08/19/2002, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

High-Resolution Physical Map For Chromosome 16q12.1-Q13, The Blau Syndrome Locus., Xiaoju Wang, Helena Kuivaniemi, Gina Bonavita, Charlene J Williams, Gerard Tromp

Department of Medicine Faculty Papers

BACKGROUND: The Blau syndrome (MIM 186580), an autosomal dominant granulomatous disease, was previously mapped to chromosome 16p12-q21. However, inconsistent physical maps of the region and consequently an unknown order of microsatellite markers, hampered us from further refining the genetic locus for the Blau syndrome. To address this problem, we constructed our own high-resolution physical map for the Blau susceptibility region. RESULTS: We generated a high-resolution physical map that provides more than 90% coverage of a refined Blau susceptibility region. The map consists of four contigs of sequence tagged site-based bacterial artificial chromosomes with a total of 124 bacterial artificial chromosomes, …

Remodeling Of Organelle-Bound Actin Is Required For Yeast Vacuole Fusion, Gary Eitzen, Li Wang, Naomi Thorngren, William Wickner

Dartmouth Scholarship

Actin participates in several intracellular trafficking pathways. We now find that actin, bound to the surface of purified yeast vacuoles in the absence of cytosol or cytoskeleton, regulates the last compartment mixing stage of homotypic vacuole fusion. The Cdc42p GTPase is known to be required for vacuole fusion. We now show that proteins of the Cdc42p-regulated actin remodeling cascade (Cdc42p --> Cla4p --> Las17p/Vrp1p --> Arp2/3 complex --> actin) are enriched on isolated vacuoles. Vacuole fusion is dramatically altered by perturbation of the vacuole-bound actin, either by mutation of the ACT1 gene, addition of specific actin ligands such as latrunculin …

The Signaling Pathway Of Oxysterol Induced Apoptosis In Chinese Hamster Ovary (Cho)-K1 Cells., Lin Yang

Electronic Theses and Dissertations

Apoptosis, a form of genetically programmed cell death, plays a key role in regulation of cellularity of the arterial wall. During atherogenesis, improper apoptosis may cause abnormalities of arterial morphogenesis, wall structural stability, and metabolisms. It has been well established that vascular cells undergo apoptosis after uptake of oxidized low-density lipoprotein (oxLDL). Thus, an analysis of the signaling pathway of apoptotic induction by oxLDL is of value in understanding the development of atherosclerotic plaque. In order to elucidate the signaling pathway of apoptosis induced by oxLDL, we have used Chinese hamster ovary (CHO)-K1 cells treated with a potent oxysterol, 25-hydroxycholesterol …

Hiv Tat Protein Activates Endothelial Cells Through Nfκb And Map Kinase Pathways., Jason L. Henry

Electronic Theses and Dissertations

HIV infection has been shown to predispose patients to accelerated development of heart disease. One mechanism for this pathology may involve endothelial activation either by HIV itself or by its secreted proteins, gp120 (a viral envelope protein) and tat (a protein that upregulates transcription of viral genes). We have studied the effects of gp120 and tat on signaling and production of inflammatory cytokines by Human Pulmonary Artery Endothelial Cells (HPAEC). HPAEC were stimulated at varying time points with combinations of gp120, tat, and monokines (IL-1β and TNFα). Cell lysate fractions were analyzed for MAP Kinase activity and NFκB activation, and …

Kalirin Dbl-Homology Guanine Nucleotide Exchange Factor 1 Domain Initiates New Axon Outgrowths Via Rhog-Mediated Mechanisms, Victor May, Martin R. Schiller, Betty A. Eipper, Richard E. Mains

Life Sciences Faculty Research

The large multidomain Kalirin and Trio proteins containing dual Rho GTPase guanine nucleotide exchange factor (GEF) domains have been implicated in the regulation of neuronal fiber extension and pathfinding during evelopment. In mammals, Kalirin is expressed predominantly in the nervous system, whereas Trio, broadly expressed throughout the body, is expressed at a lower level in the nervous system. To evaluate the role of Kalirin in fiber initiation and outgrowth, we microinjected cultured sympathetic neurons with vectors encoding Kalirin or with Kalirin antisense oligonucleotides, and we assessed neuronal fiber growth in a serum-free, satellite cell-free environment. Kalirin antisense oligonucleotides blocked the …

Experimental Autoimmune Encephalitis And Inflammation In The Absence Of Interleukin-12, Burkhard Becher, Brigit G. Durell, Randolph J. Noelle

Dartmouth Scholarship

IL-12 is considered a critical proinflammatory cytokine for autoimmune diseases such as multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). IL-12 is a heterodimer composed of a p35 subunit and a common p40 subunit shared by other cytokines. Both IL-12 p40^–/– and p35^–/– mice fail to produce IL-12 p70 heterodimer. However, in contrast to p40^–/– mice, p35^–/– mice are highly susceptible to the induction of EAE, establishing that IL-12 p70 is not essential for the development of EAE. When compared with wild-type mice, both p40^–/– and p35^–/– mice show deficiencies in primary …

Louisiana State University Health Sciences Center - 2002 - 121st Commencement, Office Of The Registrar, Lsu Health Sciences Center-No

Graduation & Commencement Programs

The program for the 121st Commencement of LSU Health Sciences Center - New Orleans held Aug 10, 2002.

Ada News - 08/05/2002, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Glutathione Levels And Bax Activation During Apoptosis Due To Oxidative Stress In Cells Expressing Wild-Type And Mutant Cystic Fibrosis Transmembrane Conductance Regulator, Thomas Jungas, Iris Motta, Francis Duffieux, Pascale Fanen, Veronique Stoven, David M. Ojcius

All Dugoni School of Dentistry Faculty Articles

Cystic fibrosis is characterized by chronic inflammation and an imbalance in the concentrations of alveolar and lung oxidants and antioxidants, which result in cell damage. Modifications in lung glutathione concentrations are recognized as a salient feature of inflammatory lung diseases such as cystic fibrosis, and glutathione plays a major role in protection against oxidative stress and is important in modulation of apoptosis. The cystic fibrosis transmembrane conductance regulator (CFTR) is permeable to Cl−, larger organic ions, and reduced and oxidized forms of glutathione, and the ΔF508 CFTR mutation found in cystic fibrosis patients has been correlated with impaired glutathione transport …

Information Interface - Volume 30, Issue 4 - August/September 2002, George Washington University, Himmelfarb Health Sciences Library

Information Interface (1976 - 2009)

News and information about Himmelfarb Health Sciences Library of interest to users.

Nuclear Transformation Of Chlamydomonas Reinhardtii To Allow For Studies On Circadian Expression Of The Β-Tubulin Gene, Mohammed Khan

Masters Theses & Specialist Projects

Circadian rhythms are rhythms that are capable of free-running in constant conditions with a period of approximately 24 hours, that are able to compensate temperature effects on their periods, and that have the ability to entrain to daily light-dark and/or temperature cycle. In order to explore the circadian output pathway that regulates β-tubulin gene expression in Chlamydomonas reinhardtii, a nuclear transformation of the organism was performed with a β-tubulin promoter:reporter gene construct. To increase the efficiency of the selection, a cotransformation procedure was adapted with ble, an antibiotic resistance gene. To achieve maximum transformation efficiency, a cell wall-less mutant of …

Distinct Properties Of Crac And Mic Channels In Rbl Cells, J. Ashot Kozak, Hubert H. Kerschbaum, Michael D. Cahalan

Neuroscience, Cell Biology & Physiology Faculty Publications

In rat basophilic leukemia (RBL) cells and Jurkat T cells, Ca²⁺ release–activated Ca²⁺ (CRAC) channels open in response to passive Ca²⁺ store depletion. Inwardly rectifying CRAC channels admit monovalent cations when external divalent ions are removed. Removal of internal Mg²⁺ exposes an outwardly rectifying current (Mg²⁺-inhibited cation [MIC]) that also admits monovalent cations when external divalent ions are removed. Here we demonstrate that CRAC and MIC currents are separable by ion selectivity and rectification properties: by kinetics of activation and susceptibility to run-down and by pharmacological sensitivity to external Mg²⁺, spermine, and …

Estimating Population Numbers Of Black Bears (Ursus Americanus) In Eastern Kentucky Using Microsatellite Analysis, Kelly Vowels

Masters Theses & Specialist Projects

Black bears (Ursus americanus) occur sporadically in eastern Kentucky, and there is some evidence that a breeding population exists. In order to establish management practices to enhance the black bear population in Kentucky, information about this population is needed. However, until recently, no population size estimate has been available. Gathering information on black bears is difficult because black bears are elusive animals. The development of new molecular methods has made it easier to track and gather information on black bear populations, including estimates of population size. Molecular markers are particularly useful in that they do not require physical contact with …

Human Exposure To Herpesvirus B–Seropositive Macaques, Bali, Indonesia, Gregory A. Engel, Lisa Jones-Engel, Michael A. Schillaci, Komang Gde Suaryana, Artha Putra, Agustin Fuentes, Richard Henkel

All Faculty Scholarship for the College of the Sciences

Herpesvirus B (Cercopithecine herpesvirus 1) has been implicated as the cause of approximately 40 cases of meningoencephalitis affecting persons in direct or indirect contact with laboratory macaques. However, the threat of herpesvirus B in nonlaboratory settings worldwide remains to be addressed. We investigated the potential for exposure to herpesvirus B in workers at a “monkey forest” (a temple that has become a tourist attraction because of its monkeys) in Bali, Indonesia. In July 2000, 105 workers at the Sangeh Monkey Forest in Central Bali were surveyed about contact with macaques (Macaca fascicularis). Nearly half of those interviewed had …

Digest Of The Philadelphia College Of Osteopathic Medicine (Fall 2002), Philadelphia College Of Osteopathic Medicine

Digest

The Fall 2002 Digest of the Philadelphia College of Osteopathic Medicine includes the following articles:

• Opening Remarks
• PCOM Updates
• Alumnus Marks 100th Birthday
• Cover Story: College Moves Forward with Strategic Plan
• Aging and the Immune Response
• Class Notes
• In Memoriam
• My Turn
• 2003 Annual Fund
• PCOM Events

Vital Signs, Fall 2002, Boonshoft School Of Medicine

Vital Signs

A twenty-seven page newsletter created by the Boonshoft School of Medicine to document the current affairs of the school. This issue includes a variety of feature articles, class notes, a calendar of events, and more.

Flight Physician - August, 2002, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Plagiocephaly: Developmental And Medical Outcomes, José Leonardo Fuentes

Loma Linda University Electronic Theses, Dissertations & Projects

Plagiocephaly, a condition that is observed by deformation of the head, comes as the result of different etiologies. The most serious involving premature fusion of one or more of the cranial sutures, a condition referred to as craniosynostosis. While children with craniosynostosis may be considered for developmental screening to determine whether they fall at risk for developmental delay, children with plagiocephaly are typically not seen as being at risk, their condition typically treated as a cosmetic problem. This study consisted of 85 children, 37 with plagiocephaly, 9 with craniosynostosis, and 39 nonclinical subjects. Results of this study suggest that children …

A Correlational Analysis Of Anthropometric Measures And Selected Blood, Immune And Endocrine Indices In Adult Males, Janet Tracey Peterson

Loma Linda University Electronic Theses, Dissertations & Projects

Over the past few decades it has become apparent that increased body weight, specifically body fat, coupled with a sedentary lifestyle is associated with an increased risk for chronic disorders such as cardiovascular disease and diabetes. Researchers are now re-directing their focus on determining the cause of these relationships from an epidemiological view to a more biological or physiological standpoint. The purpose of this cross sectional study was to examine associations among obesity, physical activity and select immune, endocrine and metabolic parameters identified as risk factors for several chronic conditions. Ninety-one healthy males between the ages of 19 and 45 …

Comparative Methods Of Measurement To Evaluate Onplant Assisted Anchorage, Marc Reid Larson

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to compare the two-dimensional cephalometric and the three-dimensional tomographic radiographic methods in their ability to assess anatomic measurements while using the Onplant anchoring device in anterior retraction cases.

The Onplant was surgically placed beneath the periosteum of the hard palate. After osseointegration the Onplant was then uncovered and attached to the first molars by means of a transpalatal bar. Conventional orthodontic treatment was then used to retract the anterior teeth after 1^st premolars were extracted.

To evaluate the movement of the Onplant, a three-dimensional measuring tool was needed. Seven different measurements were made …

Il-10 And Alveolar Bone Loss, Abdulaziz Saud Al-Rasheed

Loma Linda University Electronic Theses, Dissertations & Projects

Interleukin-10 (IL-10) is a cytokine with anti-inflammatory activities. IL-10 knockout (KO) mice exhibit severe intestinal inflammation in the presence of normal gut flora. We hypothesized that IL-10 KO mice would also be susceptible to inflammatory periodontal disease resulting in alveolar bone loss (ABL). The purpose of this study was to compare naturally occurring ABL in IL-10 KO and wild type (WT) mice. Twelve IL-10 KO and 12 WT mice, age-(7 months) and sex-(male) matched, were used. Half of the mice were on 129/SvEv and half on C57BL/6J background. Animal heads were defleshed and treated to remove all organic material. ABL …

Identification Of Hard Tissue Using Dentin Sialoprotein (Dsp) As A Marker, Wallis E. Andeline

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to identify the early hard tissue formed in experimental pulp exposures capped with calcium hydroxide, mineral trioxide aggregate (MTA) or bone morphogenetic protein-7 (BMP-7) using dentin sialoprotein (DSP) as a marker. The pulps of eighty-five maxillary first, second and third molar teeth from twenty male rats were experimentally exposed. The exposed pulps were capped with calcium hydroxide followed by restoration with amalgam (n = 20), with MTA alone as a pulp capping agent and final restoration (n = 20) or with BMP-7 followed by restoration with MTA (n = 20). Ten teeth with Class …