Digital Commons Network^™

Early Growth Response Gene 1–Mediated Apoptosis Is Essential For Transforming Growth Factor Β1–Induced Pulmonary Fibrosis, Chun Geun Lee, Soo Jung Cho, Min Jong Kang, Svetlana P. Chapoval, Patty J. Lee, Paul W. Noble, Teshome Yehualaeshet, Binfeng Lu, Richard A. Flavell, Jeffrey Milbrandt, Robert J. Homer, Jack A. Elias

Open Access Publications

No abstract provided.

Intravenous 2-Deoxy-D-Glucose Injection Rapidly Elevates Levels Of The Phosphorylated Forms Of P44/42 Mitogen-Activated Protein Kinases (Extracellularly Regulated Kinases 1/2) In Rat Hypothalamic Parvicellular Paraventricular Neurons, Arshad Khan, Alan Watts

Selected Works Temporary Series

No abstract provided.

Pressure Ulcer Development And Prevention In Long-Term Care Facilities In Virginia: A Descriptive Survey, Audrey D. Arthur

Community & Environmental Health Theses & Dissertations

The development of pressure ulcers is a common occurrence among older persons who may be temporarily immobilized or confined to a bed. An estimated 60,000 deaths annually can be attributed to complications from pressure ulcers. Guidelines recommended by the Agency for Health Care Policy and Research have been disseminated nationally and continue to be recommended as the standard of care for pressure ulcer prevention. In spite of the use of the recommended guidelines, pressure ulcer rates persist, suggesting there are other factors contributing to pressure ulcer development in long-term care facilities.

A survey was conducted to investigate which provider related …

Cytoanalysis Of Pancreatic B-Cells: Using An Avian Model, Mammalian Tissue Culture And Implications Of Antisense Oligonucleotides Transfection, Ayman Salah-El-Deen Amer

Theses, Dissertations and Capstones

Calbindin-D_28k (CaBP_28K) is a vitamin D-dependent calcium-binding protein that may alter intracellular calcium ion levels, [Ca²⁺]i. This dissertation describes experiments done to gain an understanding of the potential role of CaBP_28k in pancreatic B-cells in control of insulin secretion. The localization of CaBP_28k and insulin in chicken pancreas are shown in Chapter 1. CaBP_28k expression was found to be highest in ventral and dorsal lobes and lowest in splenic lobe. Insulin concentrations were distributed similarly among these lobes. Confocal microscopic studies demonstrated colocalization of insulin and CaBP_28k in Bcells. These findings …

Animals And Alternatives: Societal Expectations And Scientific Need, Alan M. Goldberg

Experimentation Collection

As Russell and Burch suggested more than 40 years ago, the most humane science is the best science. The path ahead is clear: pain and distress must be eliminated in animal experiments or reduced to an absolute minimum, and, as scientists, we must use the most humane approaches in our research. To accomplish the best science, we must train those who come after us in the principles and practice of humane science.

Trials, Tribulations, And Trends In Tumor Modeling In Mice, Joann C. L. Schuh

Toxicology and Animal Models in Research Collection

Selection of mouse models of cancer is often based simply on availability of a mouse strain and a known compatible tumor. Frequently this results in use of tumor models long on history but short on homology and quality control. Other factors including genetics, sex, immunological status, method and site of tumor implantation, technical competence, biological activity of the tumor, protocol sequence and timing, and selection of endpoints interact to produce outcomes in tumor models. Common reliance on survival and tumor burden data in a single mouse model often skews expectations towards high remission and cure rates; a finding seldom duplicated …

Trials, Tribulations, And Trends In Tumor Modeling In Mice, Joann C. L. Schuh

Biomedicine and Animal Models in Research Collection

Selection of mouse models of cancer is often based simply on availability of a mouse strain and a known compatible tumor. Frequently this results in use of tumor models long on history but short on homology and quality control. Other factors including genetics, sex, immunological status, method and site of tumor implantation, technical competence, biological activity of the tumor, protocol sequence and timing, and selection of endpoints interact to produce outcomes in tumor models. Common reliance on survival and tumor burden data in a single mouse model often skews expectations towards high remission and cure rates; a finding seldom duplicated …

Polymorphisms In The Prion Precursor Functional Gene But Not The Pseudogene Are Associated With Susceptibility To Chronic Wasting Disease In White-Tailed Deer, Katherine I. O'Rourke, Terry R. Spraker, Linda K. Hamburg, Thomas E. Besser, Kelly A. Brayton, Donald P. Knowles

Zoonotics and Wildlife Disease: Publications

Chronic wasting disease (CWD) status and PrP genotypes were determined for a group of 133 wild white-tailed deer in a 780 acre enclosure in western Nebraska, USA. Approximately half of the deer tested showed evidence of PrP^d in the brainstem or lymphoid tissues. Four PRNP alleles encoding amino acid substitutions were identified, with substitutions at residues 95 (Q→H), 96 (G→S) or 116 (A→G), each with serine (S) at residue 138. In addition, a processed pseudogene with two alleles encoding five or six copies of the octapeptide repeat was identified in 26% of the deer. Both alleles encoded asparagine (N) …

Transmission Of Transmissible Mink Encephalopathy To Raccoons (Procyon Lotor) By Intracerebral Inoculation, Amir N. Hamir, Janice M. Miller, Katherine I. O'Rourke, Jason C. Bartz, Mick J. Stack, Melanie J. Chaplin

Zoonotics and Wildlife Disease: Publications

To determine the transmissibility of transmissible mink encephalopathy (TME) agent to raccoons and to provide information about clinical course, lesions, and suitability of currently used diagnostic procedures for detection of transmissible spongiform encephalopathies (TSEs) in raccoons, 4 raccoon kits were inoculated intracerebrally with a brain suspension from mink experimentally infected with TME. One uninoculated raccoon kit served as a control. All 4 animals in the TME-inoculated group showed clinical signs of neurologic disorder and were euthanized between 21 and 23 weeks postinoculation (PI). Necropsy examinations revealed no gross lesions. Spongiform encephalopathy was observed by light microscopy, and the presence of …

Variable Patterns Of Distribution Of Prpcwd In The Obex And Cranial Lymphoid Tissues Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Subclinical Chronic Wasting Disease, T. R. Spraker, A. Balachandran, D. Zhuang, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Sections of medulla oblongata. taken at the level of the obex, palatine tonsil and medial retropharyngeal lymph node from 10,269 captive Rocky Mountain elk (Cervus elaphus nelsoni), were examined by immunohistochemical staining with monoclonal antibody for the prion protein associated with the transmissible spongiform encephalopathy of cervids, chronic wasting disease (PrP^CWD). The protein was detected in 226 of them. On the basis of the anatomical location of the deposits in the brainstem of 183 elk, four distinct patterns of distribution of PrP^CWD within the parasympathetic region of the dorsal motor nucleus of the vagus nerve …

Australian Children Of Alcoholic Female Twins, Wendy S. Slutske

Posters

No abstract provided.

Neurologically Plausible Distinctions In Cognition Relevant To Drug Use Etiology And Prevention, Alan W. Stacy, Susan L. Ames, Barbara J. Knowlton

CGU Faculty Publications and Research

This article outlines several distinctions in cognition and related topics in emotion that receive support from work in cognitive neuroscience and have important implications for prevention: implicit cognition, working memory, nonverbal memory, and neurobiological systems of habit. These distinctions have not been widely acknowledged or applied in drug use prevention research, despite their neural plausibility and the availability of methods to make this link. The authors briefly review the basis for the distinctions and indicate general implications and assessment possibilities for prevention researchers conducting large-scale field trials. Subsequently, the article outlines a connectionist framework for specific applications in prevention interventions. …

A Transdisciplinary Focus On Drug Abuse Prevention: An Introduction, Steve Sussman, Alan W. Stacy, C. Anderson Johnson, Mary Ann Pentz, Elizabeth Robertson

CGU Faculty Publications and Research

This article introduces the scope of the Special Issue. A variety of scientific disciplines are brought together to establish theoretical integration of the arenas of drug use, misuse, “abuse,” and drug misuse prevention. Transdisciplinary scientific collaboration (TDSC) is utilized as a process of integration. Introductory comments regarding the strengths and limitations of TDSC are presented. Then, the relevance of genetics to substance misuse and substance misuse prevention is presented. Next, the relevance of cognition for prevention is discussed. Specifically, neurologically plausible distinctions in cognition and implicit cognition and their relevance for prevention are discussed. At a relatively molar social-level of …

Flight Physician - January, 2004, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Santa Clara Magazine, Volume 46 Number 3, Winter 2004, Santa Clara University

Santa Clara Magazine

8 - AN ARTIST WITH A MISSION By Elizabeth Kelley Gillogly '93. In his latest series, Howard Anderson '71 created paintings of the 21 California missions.

10 - THE SPIRITUALITY OF GIFT GIVING By Tom Beaudoin. Is it possible to turn our gift giving into a spiritual exercise?

14 - RAISING ARIZONA By Susan Shea. Arizona Governor Janet Napolitano '79, a rising political star, uses the lessons learned at SCU to serve her state.

20 - LEAVING COMMUNISM BEHIND By Jane Curry. An SCU professor and Fulbright Scholar researches forgiveness and justice in post-communist Poland.

College Of Optometry Program Overview, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Chronic Medical Illness : The Counselor's Role, Diana J. Cira

Graduate Research Papers

In the next few decades, the number of chronically medically ill individuals in the United States will increase dramatically. To implement necessary interventions to improve their care, the United States health care system must make changes in the approaches used for these individuals. Counselors can play a critical role in these changes. By incorporating counselors in the care of individuals with chronic medical illnesses, the health care system will be better able to provide interventions that aid in effectively coping with the illnesses and the many psychosocial ramifications of the illnesses.

Book Review: Shane P. Desselle, Dana P. Hammer. Handbook For Pharmacy Educators: Getting Adjusted As A New Pharmacy Faculty Member. Binghamton, Ny: Pharmaceutical Products Press. 88 Pp (Hardcover) $24.95, Eric G. Boyce

School of Pharmacy Faculty Articles

No abstract provided.

Once Upon A Virus: Aids Legends And Vernacular Risk Perception, Diane E. Goldstein

All USU Press Publications

Once Upon a Virus explores how contemporary, or "urban," legends are indicators of culturally complex attitudes toward health and illness. Tracing the rich tradition of AIDS legends in relation to current scholarship on belief, Diane Goldstein shows how such stories not only articulate widespread perceptions of risk, health care, and health policy, they also influence official and scientific approaches to the disease and its management. Notions that appear in narratives of who gets AIDS, how and why, are indicators of broad issues involving health beliefs, concerns, and needs.

Graphical Technique For Identifying A Monotonic Variance Stabilizing Transformation For Absolute Gene Intensity Signals, Kellie J. Archer, Catherine I. Dumur, Viswanathan Ramakrishnan

Biostatistics Publications

Background

The usefulness of log₂ transformation for cDNA microarray data has led to its widespread application to Affymetrix data. For Affymetrix data, where absolute intensities are indicative of number of transcripts, there is a systematic relationship between variance and magnitude of measurements. Application of the log₂ transformation expands the scale of genes with low intensities while compressing the scale of genes with higher intensities thus reversing the mean by variance relationship. The usefulness of these transformations needs to be examined.

Results

Using an Affymetrix GeneChip^® dataset, problems associated with applying the log₂ transformation to absolute intensity …

Distinct Roles For The Aaa Atpases Nsf And P97 In The Secretory Pathway, Seema Dalal, Meredith F.N. Rosser, Douglas M. Cyr, Phyllis I. Hanson

Open Access Publications

No abstract provided.

Dinitroanilines Bind Α-Tubulin To Disrupt Microtubules, Naomi S. Morrissette, Arpita Mitra, David Sept, L. David Sibley

Open Access Publications

No abstract provided.

A Cost-Benefit Analysis Of Gown Use In Controlling Vancomycin-Resistant Enterococcus Transmission: Is It Worth The Price?, Laura A. Puzniak, Kathleen N. Gillespie, Terry Leet, Marin Kollef, Linda M. Mundy

Open Access Publications

No abstract provided.

Structural Basis For The Restoration Of Tcr Recognition Of An Mhc Allelic Variant By Peptide Secondary Anchor Substitution, Michael J. Miley, Ilhem Messaoudi, Beatrix M. Metzner, Yudong Wu, Janko Nikolich-Žugich, Daved H. Fremont

Open Access Publications

No abstract provided.

Treatment Of Terminal Peritoneal Carcinomatosis By A Transducible P53-Activating Peptide, Eric L. Snyder, Bryan R. Meade, Cheryl C. Saenz, Steven F. Dowdy

Open Access Publications

No abstract provided.

Effect Of Day Length On Sleep Habits And Subjective On-Duty Alertness In Irregular Work Schedules, June J. Pilcher, Heather M. Teichman, Stephen Popkin, Krista Hildebrand, Michael Coplen

Publications

The human circadian system is sensitive to environmental conditions, such as those created by shift work, that affect the timing and duration of sleep. Previous research on the effects of shift work, however, has focused primarily on regularly scheduled shifts. Few studies have focused on irregular and unpredictable on-call shift systems, such as those found in much of railroad operations. The purpose of the current study was to examine the effect of irregular shift systems experienced by locomotive engineers on the length of sleep-wake periods and the effect of different length sleep-wake periods on self-reported sleep quantity, sleep quality, and …

Biology Of PrpSc Accumulation In Two Natural Scrapie-Infected Sheep Flocks, Patrick Caplazi, Katherine I. O'Rourke, Cynthia Wolf, Daniel Shaw, Timothy V. Baszler

Zoonotics and Wildlife Disease: Publications

Sheep scrapie is a prion disease that requires interaction of exogenous prions with host prion protein (PrP) supporting prion formation. Disease is associated with deposition of a host-generated conformational variant of PrP, PrP^sc, in a variety of tissues, including brain, resulting in fatal spongiform encephalopathy. Efficiency of PrP^sc formation is determined by polymorphisms in the PrP-coding sequence. This article adds to previous data of natural sheep scrapie, concentrating on the effect of host genotype and age on PrP^sc accumulation patterns during preclinical and clinical disease. Two entire scrapie-infected, predominantly Suffolk-cross, sheep flocks euthanized for regulatory purposes …

Cognitive-Behavioral Therapy, Light Therapy, And Their Combination In Treating Seasonal Affective Disorder, Kelly J. Rohan, Kathryn Tierney Lindsey, Katheryn A. Roecklein, Timothy J. Lacy

Uniformed Services University of the Health Sciences

Background: The need to develop supplementary or alternative treatments for seasonal affective disorder (SAD) is underscored by the significant minority (47%) of SAD patients that is refractory to light therapy, the persistence of residual symptoms despite light treatment, and poor long-term compliance with light use. Because preliminary studies suggest that cognitive and behavioral factors are involved in SAD, cognitive-behavioral therapy (CBT) warrants investigation as a possible treatment option. Methods: We piloted a 6-week randomized clinical trial to compare a standard light therapy protocol; a novel, SAD-tailored, group CBT intervention; and their combination in ameliorating and remitting a current SAD episode …

Functional Characterization Of The Arabidopsis Thaliana Orthologue Of Tsc13p, The Enoyl Reductase Of The Yeast Microsomal Fatty Acid Elongating System, Kenneth Gable, Sarah Garton, Johnathan A. Napier, Teresa M. Dunn

Uniformed Services University of the Health Sciences

The protein encoded by the Arabidopsis At3g55360 gene was selected as a candidate for the enoyl reductase of the microsomal elongase system based on its homology to the Tsc13p protein of S. cerevisiae. The studies presented here demonstrate that heterologous expression of At3g55360 functionally complements the temperature-sensitive phenotype of a yeast tsc13 mutant that is deficient in enoyl reductase activity. Furthermore, AtTSC13 is shown to interact physically with the Elo2p and Elo3p components of the yeast elongase complex. At3g55360 apparently encodes the sole enoyl reductase activity associated with microsomal fatty acid elongation in Arabidopsis. Consistent with this conclusion, …

Structure Of Shiga Toxin Type 2 (Stx2) From Escherichia Coli O157:H7, Marie E. Fraser, Masao Fujinaga, Maia M. Cherney, Angela R. Melton-Celsa, Edda M. Twiddy, Alison D. O'Brien, Michael N. G. James

Uniformed Services University of the Health Sciences

Several serotypes of Escherichia coli produce protein toxins closely related to Shiga toxin (Stx) from Shigella dysenteriae serotype 1. These Stx-producing E. coli cause outbreaks of hemorrhagic colitis and hemolytic uremic syndrome in humans, with the latter being more likely if the E. coli produce Stx2 than if they only produce Stx1. To investigate the differences among the Stxs, which are all AB₅ toxins, the crystal structure of Stx2 from E. coli O157:H7 was determined at 1.8-Å resolution and compared with the known structure of Stx. Our major finding was that, in contrast to Stx, the active site of …