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Nucleic Acid Encoding Human Rev1 Protein, Zhigang Wang, Winston Lin, Hua Xin, Xiaohua Wu

Toxicology and Cancer Biology Faculty Patents

The present invention relates to a human cDNA homologous to the yeast REV1 gene. The sequence of human REV1 (hREV1) gene is described.

Effect Of Escherichia Coli And Lactobacillus Rhamnosus On Macrophage Inflammatory Protein 3Α, Tumor Necrosis Factor Alpha, And Transforming Growth Factor Β Release By Polarized Rat Uterine Epithelial Cells In Culture, M. A. Crane-Godreau, C. R. Wira

Dartmouth Scholarship

Entry of bacteria from the vagina into the uterus raises the question of uterine epithelial cell (UEC) signaling in response to the presence of bacteria. Our model system helps to define microbially elicited UEC basolateral cytokine release, important in regulating underlying stromal immune cell protection. UECs from adult rats were grown in cell culture inserts to establish a confluent polarized monolayer as was determined by transepithelial resistance (TER). Polarized epithelial cell cultures were treated apically with live or heat-killed Escherichia coli or Lactobacillus rhamnosus prior to collection of basolateral media after 24 h of incubation. Coculture of polarized UECs with …

Amyloid-Beta Peptide Induces Oligodendrocyte Death By Activating The Neutral Sphingomyelinase-Ceramide Pathway, Jiunn-Tay Lee, Jan Xu, Jin-Moo Lee, Grace Ku, Xianlin Han, Ding-I Yang, Shawei Chen, Chung Y. Hsu

Open Access Publications

No abstract provided.

Trafficking Of Plasmepsin Ii To The Food Vacuole Of The Malaria Parasite Plasmodium Falciparum, Michael Klemba, Wandy Beatty, Ilya Gluzman, Daniel E. Goldberg

Open Access Publications

No abstract provided.

Bcma Is Essential For The Survival Of Long-Lived Bone Marrow Plasma Cells, Brian P. O'Connor, Vanitha S. Raman, Loren D. Erickson, W. James Cook, Lehn K. Weaver, Cory Ahonen, Ling-Li Lin, George Mantchev, Richard J. Bram, Randolph J. Noelle

Dartmouth Scholarship

Long-lived humoral immunity is manifested by the ability of bone marrow plasma cells (PCs) to survive for extended periods of time. Recent studies have underscored the importance of BLyS and APRIL as factors that can support the survival of B lineage lymphocytes. We show that BLyS can sustain PC survival in vitro, and this survival can be further enhanced by inter- leukin 6. Selective up-regulation of Mcl-1 in PCs by BLyS suggests that this 􏰀-apoptotic gene product may play an important role in PC survival. Blockade of BLyS, via transmembrane activator and cyclophilin ligand interactor–immunoglobulin treatment, inhibited PC survival in …

Role Of The Hydrophobic Domain In Targeting Caveolin-1 To Lipid Droplets, Anne G. Ostermeyer, Lynne T. Ramcharan, Youchun Zeng, Douglas M. Lublin, Deborah A. Brown

Open Access Publications

No abstract provided.

What Twin Studies Teach Us About The Causes Of Alcoholism, Carol A. Prescott

Presentations

No abstract provided.

Craving Alcohol: Genetic And Pharmacological Factors, Kent Hutchison

Presentations

No abstract provided.

Calcium And Glycolysis Mediate Multiple Bursting Modes In Pancreatic Islets, Richard Bertram, Leslie S. Satin, Min Zhang, Paul Smolen, Arthur Sherman

Pharmacology and Toxicology Publications

Abstract

Pancreatic islets of Langerhans produce bursts of electrical activity when exposed to stimulatory glucose levels. These bursts often have a regular repeating pattern, with a period of 10–60 s. In some cases, however, the bursts are episodic, clustered into bursts of bursts, which we call compound bursting. Consistent with this are recordings of free Ca²⁺ concentration, oxygen consumption, mitochondrial membrane potential, and intraislet glucose levels that exhibit very slow oscillations, with faster oscillations superimposed. We describe a new mathematical model of the pancreatic β-cell that can account for these multimodal patterns. The model includes the feedback of …

Serine-Threonine Kinases And Transcription Factors Active In Signal Transduction Are Detected At High Levels Of Phosphorylation During Mitosis In Preimplantation Embryos And Trophoblast Stem Cells, Jian Liu, Elizabeth E. Puscheck, Fangfei Wang, Anna Trostinskaia, Dusan Barisic, Gordon Maniere, Dana Wygle, W Zhong, Edmond Hhm Rings, Daniel A. Rappolee

Department of Obstetrics and Gynecology Research Publications

Serine-threonine kinases and transcription factors play important roles in the G1-S phase progression of the cell cycle. Assays that use quantitative fluorescence by immunocytochemical means, or that measure band strength during Western blot analysis, may have confused interpretations if the intention is to measure G1-S phase commitment of a small subpopulation of phosphorylated proteins, when a larger conversion of the same population of proteins can occur during late G2 and M phases. In mouse trophoblast stem cells (TSC), a human placental cell line (HTR), and/or mouse preimplantation embryos, 8/19 ser- ine-threonine and tyrosine kinases, 3/8 transcription factors, and 8/14 phospho …

Download Full Pdf Issue-Jefferson Translations, Vol. 2, No. 1, Fall/Winter 2004-2005

Jefferson Translations

This issue includes an article on Jefferson's Heart Failure center, a highlight on Dr. Eckhart of Translational Medicine, faculty news and awards, a report on strong outcomes for liver transport team, the latest in asthma research and a master's program in human investigation.

Palifermin For Oral Mucositis After Intensive Therapy For Hematologic Cancers, Ricardo Spielberger, Patrick Stiff, William Bensinger, Teresa Gentile, Daniel Weisdorf, Tarun Kewalramani, Thomas Shea, Saul Yanovich, Keith Hansen, Stephen Noga, John Mccarty, C. Frederick Lemaistre, Eric C. Sung, Bruce R. Blazar, Dieter Elhardt, Mon-Gy Chen, Christos Emmanouilides

Internal Medicine Publications

BACKGROUND: Oral mucositis is a complication of intensive chemotherapy and radiotherapy with no effective treatment. We tested the ability of palifermin (recombinant human keratinocyte growth factor) to decrease oral mucosal injury induced by cytotoxic therapy. METHODS: This double-blind study compared the effect of palifermin with that of a placebo on the development of oral mucositis in 212 patients with hematologic cancers; 106 patients received palifermin (60 microg per kilogram of body weight per day) and 106 received a placebo intravenously for three consecutive days immediately before the initiation of conditioning therapy (fractionated total-body irradiation plus high-dose chemotherapy) and after autologous …

Antimicrobial And Spermicidal Activity Of Hydrogen Peroxide And Nonoxynol-9 Based Gel Preparations, Paul E. Grimmett

Theses, Dissertations and Capstones

Eighty gel formulations were prepared using five buffer systems (0.01M citrate, 0.02M citrate, 0.01M tartrate, 0.02M tartrate, and 0.01M citrate/0.01M tartrate), four hydrogen peroxide (H2O2) concentrations (0%, 0.3%, 0.9%, and 1.2%), and four nonoxynol-9 (N-9) concentrations (0%, 1%, 1.5%, and 2%). The eighty gel formulations were then tested for physio-chemical properties (viscosity and pH), antimicrobial effectiveness (Minimum Inhibitory Concentration Assay) against Candida albicans, Escherichia coli, and Streptococcus agalactiae, and finally, spermicidal effectiveness (Sander Cramer Assay). The gel formulation that contained the best combination of physio-chemical, antimicrobial, and spermicidal properties was a 0.01M citrate buffered hydrogel, which contained 0.9% H2O2 and …

Hk-2 Cells As A Human Model Of Glucuronide Transport, Eliza E. Robertson

Theses, Dissertations and Capstones

Glucuronidation is primarily a pathway of detoxification in most species, but many glucuronide conjugates are associated with toxicity. Numerous drugs are excreted in the urine as glucuronide conjugates. Being organic anions, it is likely that glucuronides are secreted into the urine by organic anion transporters found in renal proximal tubule cells (PTCs). Some drugs that are metabolized by glucuronidation have been shown to cause renal toxicity, yet little is known about the renal handling of glucuronide conjugates. It is hypothesized that glucuronides are transported into renal PTCs by an organic anion transporter (OAT) on the basolateral membrane of the cell. …

The Genetic And Molecular Characterization Of The Polycystic Kidney Disease-Causing Mouse Gene Bicc1, Sarah J. Price

Theses, Dissertations and Capstones

Polycystic kidney disease (PKD) is one of the most common hereditary diseases and is characterized by progressive cyst formation, substantial renal enlargement, and frequently, progression to end-stage renal disease. One way to learn more about the etiology of this disease is to study mouse models that imitate the human situation. The juvenile congenital polycystic kidney disease (jcpk) gene on mouse Chromosome 10 has been found to cause a severe, early onset form of PKD when inherited in an autosomal recessive manner (Flaherty et al., 1995). Previous genetic studies mapped the jcpk locus to a 1 cM region on mouse Chromosome …

The Use Of Ergogenic Aids Among High School Athletes In Eastern Kentucky, John Robert Brandenburg

Theses, Dissertations and Capstones

A fifteen-question survey was developed to study the use of over-the counter nutrition supplements by high school athletes in eastern Kentucky. Athletic trainers from four local high schools were given one hundred permission forms and surveys. After two weeks only a small number (n=25) completed the requirements to participate in the study. The twenty-five participants consisted of male (n=15) and female (n=10). The athletes with the highest frequency of supplement use were football players (67.7%). The supplements with the highest use were protein powders (n=13) and creatine monohydrate (n=9).

Roles Of Pkc Isozymes In Retinoic Acid-Induced B16 Mouse Melanoma Cell Growth Inhibition And Differentiation, Jianming Han

Theses, Dissertations and Capstones

Protein Kinase C (PKC) has been implicated in many cellular responses. Of the 11 isotypes in this family, seven are expressed in B16 mouse melanoma cells − PKCα, PKCδ, PKCε, PKCθ, PKCλ, PKCζ, and PKCµ.

Treatment of B16 cells with retinoic acid (RA) results in growth inhibition and differentiation. Simultaneously, RA induces a 6-8 fold increase of PKC α protein level, but only about 2-fold increase of PKC enzyme activity. The over- expression of wild type PKCα in B16 cells mimicked the action of RA. The over- expression of enzymatically inactive PKCα, either open or closed conformation, also inhibited B16 …

Docosahexaenoic Acid Modulates Class I Major Histocompatibility Complex Protein Function, Kaleb Marie Hypes

Theses, Dissertations and Capstones

Fatty acids influence immune responses, but how this occurs at the molecular level is not understood. Class I major histocompatibility complex molecules (MHC I), transmembrane proteins required for antigen presentation to T cell receptors, each consist of a 45kDa α chain noncovalently associated with a 12-kDa β₂-microglobulin (β₂m) molecule. Docosahexaenoic acid’s (DHA) effect on the binding of β₂m and anti-MHC I monoclonal antibodies (Mab) was measured. EL4 and RMA-S cells were cultured 48 hours with 0-45µM DHA or oleic acid. Murine to human β₂m exchange and Mab binding were assessed by fluorescence …

Information Interface - Volume 32, Issue 1 - January/February 2004, George Washington University, Himmelfarb Health Sciences Library

Information Interface (1976 - 2009)

News and information about Himmelfarb Health Sciences Library of interest to users. Includes articles on offsite journals, added features to MEDLINE/PubMed, new journal titles, and database search tips.

Articulations 2004, Som School Of Osteopathic Medicine, Umdnj University Of Medicine And Dentistry Of Nj

Yearbooks

University of Medicine and Dentistry of New Jersey (UMDNJ) School of Osteopathic Medicine yearbook for the class of 2004. 128 pages.

Contents: Osteopathic Oath p.1, UMDNJ-SOM Campus p. 2, University Affiliated Medical Centers p. 3, Dean's Message p. 4, Administration p. 5, Faculty p. 6, Staff p. 15, Candids p. 18, Graduates p. 34, Senior Party p. 112, Convocation and Commencement p. 113, Editor's Page p. 120.

Occupational Therapy Program 2004, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Dentalgram - Vol. 18, No. 01, Loma Linda University School Of Dentistry

Dentalgram

In this issue:

• Kathleen Moore named assistant dean
• New appointments
• Alumni Student Convention, 2004
• Department news
• Early selection program agreement with Antillean Adventist University
• Dean's List for autumn quarter
• In memoriam - Erwin A. McDonald, SD'61

Developing A Scale To Measure Just About Anything: Comparisons Across Groups And Individuals, Katharine Fast

Yale Medicine Thesis Digital Library

No abstract provided.

College Of Pharmacy Student Handbook June 2004, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

College Of Osteopathic Medicine Program Overview, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Using Lc-Ms With De Novo Software To Fully Characterize The Multiple Methylations Of Lysine Residues In A Recombinant Fragment Of An Outer Membrane Protein From A Virulent Strain Of Rickettsia Prowazekii, Chien-Chung Chao, Shiaw-Lin Wu, Wei-Mei Ching

Uniformed Services University of the Health Sciences

The outer membrane protein B (OmpB) of the typhus group rickettsiae is an immunodominant antigen and has been shown to provide protection against typhus in animal models. Consequently, OmpB is currently being considered as a potential rickettsiae vaccine candidate to be used in humans. The OmpB from virulent strains are heavily methylated while the attenuated strains are hypomethylated. Western blot analysis of partially digested OmpB revealed that one of the reactive fragments was located at the N-terminus (fragment A, aa 33–272). Recently, we have over expressed, purified, and chemically methylated the recombinant fragment A from Rickettsia prowazekii (Ap). The methylated …

2003 Cancer Care Annual Report, Children's Mercy Hospital

Cancer Center Annual Reports

Annual report of cancer care and oncology services at the Children's Mercy Hospital, Kansas City MO, a pediatric medical center, with a focus on AML.

The Measurement Of Adolescent Depression, Leigh Roeger

Shannon Research Press

Very broadly the general aims of this study are: to examine whether the Center for Epidemiological Studies Depression Scale (CES-D) measures depressive symptomatology equivalently across adolescent boys and girls; and to examine whether schools exert effects on student levels of depressive symptomatology independently of individual level characteristics. In the course of this study quite a number of subsidiary questions are also addressed. Most of these questions centre around the psychometric properties of the CES-D scale when used with adolescent samples.

Difficulties In Recognizing And Treating Depression In The Elderly : Implications For Counselors, Kristin A. Schloemer

Graduate Research Papers

Diagnosing elderly depression is a difficult, overlooked process. Many elderly seek out family physicians, but few visit mental healthcare professionals (Lyness et al., 1997; Sable & Dunn, 2002). When assessing elderly depression, it is important for counselors to be aware of symptoms, ensuring correct treatment.

According to Friedrich ( 1999), it is difficult for healthcare professionals, including counselors, to diagnose depression in the elderly. As elderly may not display traditional depressive symptoms, it is necessary to distinguish between depression, bereavement, and illness, all common in late life. Once depression has been diagnosed, treatments including: medication, psychotherapy, or perhaps the most …

Attachment Theory : How To Treat Attachment Disorders In Adults, Kristy L. Hrdlicka

Graduate Research Papers

Attachment is a bond formed between two individuals over a period of time, dealing with how they relate to one another through their interactions (Sable, 2000). In this paper, a review of the literature is discussed regarding how the attachments between individuals are formed and the four different patterns of attachment: Secure, anxious-ambivalent, avoidant, and disorganized. Following the review, practical interventions are also provided for counseling professionals to use when working with clients who have attachment disorders.