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Mechanisms Of Cell Death Initiated In Herpes Simplex Virus Thymidine Kinase Expressing Colon Tumor Cells Treated With Ganciclovir And Ucn-01, Christina Elizabeth Ahn

Theses and Dissertations in Biomedical Sciences

Metastatic colon carcinoma is the second leading cause of death from malignancy in the United States, and development of more effective treatments is essential. Heterologous expression of Herpes Simplex Virus Thymidine Kinase (HSVtk) in combination with the prodrug, ganciclovir (GCV), has shown great promise for the genetic therapy of many cancers, but most patients have had only a partial or minimal response to the therapy. After screening a panel of two drug combinations, our laboratory has shown that the combination of GCV and the protein kinase inhibitor UCN-01 (7-hydroxystaurosporine) enhances tumor cell death more effectively than either drug alone. However …

Bench-To-Bedside Review: Rhabdomyolysis -- An Overview For Clinicians., Ana L Huerta-Alardín, Joseph Varon, Paul E Marik

Jefferson Hospital Staff Papers and Presentations

Rhabdomyolysis ranges from an asymptomatic illness with elevation in the creatine kinase level to a life-threatening condition associated with extreme elevations in creatine kinase, electrolyte imbalances, acute renal failure and disseminated intravascular coagulation. Muscular trauma is the most common cause of rhabdomyolysis. Less common causes include muscle enzyme deficiencies, electrolyte abnormalities, infectious causes, drugs, toxins and endocrinopathies. Weakness, myalgia and tea-colored urine are the main clinical manifestations. The most sensitive laboratory finding of muscle injury is an elevated plasma creatine kinase level. The management of patients with rhabdomyolysis includes early vigorous hydration.

Insertional Polymorphisms Of Endogenous Feline Leukemia Viruses, Alfred L. Roca, William G. Nash, Joan C. Menninger, William J. Murphy, Stephen J. O'Brien

Biology Faculty Articles

The number, chromosomal distribution, and insertional polymorphisms of endogenous feline leukemia viruses (enFeLVs) were determined in four domestic cats (Burmese, Egyptian Mau, Persian, and nonbreed) using fluorescent in situ hybridization and radiation hybrid mapping. Twenty-nine distinct enFeLV loci were detected across 12 of the 18 autosomes. Each cat carried enFeLV at only 9 to 16 of the loci, and many loci were heterozygous for presence of the provirus. Thus, an average of 19 autosomal copies of enFeLV were present per cat diploid genome. Only five of the autosomal enFeLV sites were present in all four cats, and at only one …

The Linkage Disequilibrium Maps Of Three Human Chromosomes Across Four Populations Reflect Their Demographic History And A Common Underlying Recombination Pattern, Francisco M. De La Vega, Hadar Isaac, Andrew Collins, Charles R. Scafe, Bjarni V. Halldorsson, Xiaoping Su, Ross A. Lippert, Yu Wang, Marion Laig-Webster, Ryan T. Koehler, Janet S. Ziegle, Lewis T. Wogan, Junko F. Stevens, Kyle M. Leinen, Sheri J. Olson, Karl J. Guegler, Xiaoqing You, Lily H. Xu, Heinz G. Hemken, Francis Kalush, Mitsuo Itakura, Yi Zheng, Guy De The, Stephen J. O'Brien, Andrew G. Clark, Sorin Istrail, Michael W. Hunkapiller, Eugene G. Spier, Dennis A. Gilbert

Biology Faculty Articles

The extent and patterns of linkage disequilibrium (LD) determine the feasibility of association studies to map genes that underlie complex traits. Here we present a comparison of the patterns of LD across four major human populations (African-American, Caucasian, Chinese, and Japanese) with a high-resolution single-nucleotide polymorphism (SNP) map covering almost the entire length of chromosomes 6, 21, and 22. We constructed metric LD maps formulated such that the units measure the extent of useful LD for association mapping. LD reaches almost twice as far in chromosome 6 as in chromosomes 21 or 22, in agreement with their differences in recombination …

Chironian Spring/Summer 2005, New York Medical College

The Chironian

No abstract provided.

Supporting Ourselves: Maintaining Mental Well-Being Through An Hiv+/Aids Support Group In A Rural South African Community, Narda Hernández

Independent Study Project (ISP) Collection

This paper primarily explores the importance of the support group for people infected and affected by HIV+/AIDS. At St. Matthew’s Clinic in the rural area of Qoboqobo, I invited women between the ages of 25-49 to freely participate in the focus group discussions. I believe that the health care sector could benefit from these groups, both as formal outreach services and informal support networks. There is a lack of healthcare services in rural areas. Of particular interest, PLWA are continuously encouraged to join an HIV/AIDS support group. This paper aims to explore how people are dealing with stress and HIV/AIDS-related …

Reconciling Temporalities: A Substantive Explanation Of The Origins Of Difficulty In The Nurse Patient Encounter, Marilyn Theresa Macdonald Phd

Dissertations

Nurses describe patients as difficult on a regular basis. Nursing research to date has assumed the existence of this phenomenon. Most studies have listed descriptors of the difficult patient and offered interventions for nurses to use to alter patient behavior. Locating of difficulty within the individual and failure to consider the context of the nurse patient encounter is problematic. The practice of locating difficulty in the individual absolves organizations and society of responsibility to work to change factors that contribute to the construction of difficulty. The purpose of this research was to move beyond a focus on the patient as …

J Am Coll Dent 2005 72 2

Journal of the American College of Dentists

The Journal of the American College of Dentists (JACD) publishes scholarly articles, editorials, essays, and reflections addressing the ethical, professional, and social dimensions of dentistry. The Journal serves as a forum for advancing ethics, professionalism, leadership, and excellence within the dental community. By fostering dialogue across disciplines and perspectives, JACD provides readers with insights that encourage ethical, professional practice, lifelong learning, and service to the public, reinforcing dentistry’s commitment to advancing oral healthcare and the common good.

Defensin In The Ticks Dermacentor Variabilis And Ixodes Scapularis, Shane Michael Ceraul

Theses and Dissertations in Biomedical Sciences

This dissertation presents the identification, molecular structure and expression of defensin, an antimicrobial peptide, in the ticks, Dermacentor variabilis and Ixodes scapularis. A 624 bp cDNA molecule for defensin was cloned and sequenced from hemocytes, fat body and midgut of unstimulated D. variabilis ticks. Defensin peptide was detected only in the hemocyte lysates. These results suggest that the defensin from D. variabilis is synthesized and stored in the hemocytes, although the role of other tissues is not excluded.

The ability of the juvenile stage ticks to express the defensin gene was confirmed. Detection of the defensin transcript in all …

Predictors Of Student Success In An Entry-Level Baccalaureate Dental Hygiene Program, Mohammad J. Alzahrani

Dental Hygiene Theses & Dissertations

The purpose of this study was to measure the utility of a variety of predictors that have been used by the Old Dominion University, Gene W. Hirschfeld School of Dental Hygiene and other baccalaureate dental hygiene programs in selecting dental hygiene students who are most likely to graduate and be successful in passing the National Board Dental Hygiene Examination (NBDHE). Specifically this study attempted to determine whether or not the following factors are reliable predictors of success in a an entry-level baccalaureate dental hygiene program as evidenced by graduation and successful entry into the profession as evidenced by passing the …

Hospital Epidemiological Surveillance During A Major Construction Project, Sherry Sexton

Graduate Research Projects

The purposes ofthis study were: a) to detennine if organism migration occurred from a construction area into other areas of the hospital-especially adjacent operating suites, b) to monitor and evaluate the incidence of construction-related surgical site infections, and c) to monitor the incidence of employee illnesses that could be construction-related. This was a descriptive correlational study and provided epidemiological data to evaluate the effectiveness of infection control measures in preventing employee or patient infections related to construction. The framework for this study was Florence Nightingale's Environmental Adaptation theory which focused on the environment and changes which could affect patient outcomes. …

Correlates Of In-Home Smoking Behavior Of Parents With Newborns, James Kyung Jo

Loma Linda University Electronic Theses, Dissertations & Projects

Secondhand smoke has been shown to have adverse health effects on young children. It is associated with various health effects such as respiratory infections, Sudden Infant Death Syndrome (SIDS), and asthma. Because of the dangers secondhand smoke poses to young children, there is a need for educating parents about the dangers of secondhand smoke as well as determining factors associated with secondhand smoke exposure among children. Understanding these factors may be first step toward developing strategies to reduce ETS exposure.

The study examined correlates of in-home smoking behavior of parents with newborns by analyzing 657 respondents who filled out the …

Sara Is An Essential Positive Regulator Of Staphylococcus Epidermidis Biofilm Development, Maria A. Tormo, Miguel Marti, Jaione Valle, Adhar C. Manna

Dartmouth Scholarship

Staphylococcus epidermidis biofilm formation is associated with the production of the polysaccharide intercellular adhesin (PIA)--poly-N-acetylglucosamine polysaccharide (PNAG) by the products of the icaADBC operon. Recent evidence indicates that SarA, a central regulatory element that controls the production of Staphylococcus aureus virulence factors, is essential for the synthesis of PIA/PNAG and the ensuing biofilm development in this species. Based on the presence of a sarA homolog, we hypothesized that SarA could also be involved in the regulation of the biofilm formation process in S. epidermidis. To investigate this, we constructed nonpolar sarA deletions in two genetically unrelated S. epidermidis clinical strains, …

Identification Of A Tcpc-Tcpq Outer Membrane Complex Involved In The Biogenesis Of The Toxin-Coregulated Pilus Of Vibrio Cholerae, Niranjan Bose, Ronald K. Taylor

Dartmouth Scholarship

The toxin-coregulated pilus (TCP) of Vibrio cholerae and the soluble TcpF protein that is secreted via the TCP biogenesis apparatus are essential for intestinal colonization. The TCP biogenesis apparatus is composed of at least nine proteins but is largely uncharacterized. TcpC is an outer membrane lipoprotein required for TCP biogenesis that is a member of the secretin protein superfamily. In the present study, analysis of TcpC in a series of strains deficient in each of the TCP biogenesis proteins revealed that TcpC was absent specifically in a tcpQ mutant. TcpQ is a predicted periplasmic protein required for TCP biogenesis. Fractionation …

The Great Pestilence: Yellow Fever In Portsmouth, Virginia, 1855, Burden Susan Lundgren

Health Services Research Dissertations

In 1855, the town of Portsmouth, Virginia was devastated by an epidemic of yellow fever. Most citizens fled. Of those who remained, most became infected and a thousand died. The municipal government collapsed. In their place, a small organization known as the Portsmouth Relief Association assumed responsibility for ensuring the survival of the town. This organization managed the care of the sick, the burial of the dead, and the care of orphans. It was the sole agent receiving and allocating the funds and resources that poured into the community. Scarce food, drugs and other supplies were available only through the …

Cultural Competence And Curricula In Physical Therapist Professional Education, Beth Ernst Jamali

Theses and Dissertations in Urban Services - Urban Education

The purpose of this research was to examine the methods and degree to which content related to cultural competence is incorporated into current entry-level physical therapist education. Face-to-face interviews were performed with 10 program directors from various physical therapist education programs across the country to obtain in-depth information regarding cultural competence in the physical therapy curricula. A questionnaire was sent to all of the 193 accredited physical therapist education programs in the United States as a second means to collect information regarding the methods and extent to which cultural competence is included in the physical therapy curricula. Data were collected …

The Antitumor Agent, Arglabin-Dma, Preferentially Induces Apoptosis In Human Colon Tumor Cells, Sung Wook Kwon

Theses and Dissertations in Biomedical Sciences

Arglabin-DMA, an analog of farnesyl pyrophosphate (FPP), reportedly inhibits farnesyltransferase (FTase) directly by competitively blocking the binding of Ras protein and its posttranslational modification, as suggested in previous studies. But, the mechanisms by which Arglabin-DMA inhibits tumor growth in vivo and in vitro are still relatively poorly characterized. To determine the mechanism by which this drug inhibits tumor growth, the effects of Arglabin-DMA in two human colon tumor cell lines (mutant K-ras HCT 116 and wild-type ras HT-29) were explored on cell proliferation, apoptosis, and cell cycle kinetics in vitro. In cell viability studies, we showed that Arglabin-DMA …

Should Liability Play A Role In The Social Control Of Biobanks?, Larry I. Palmer

Faculty Publications

No abstract provided.

Exploring California's Rural Health System: From The Redwood Forests To The Baja Border, Eileen Salinsky, Jessamyn Taylor

National Health Policy Forum

This three day site visit to California explored the state’s rural health system with a particular focus on the financial health of inpatient and outpatient providers and how that affects access to care. The role of telemedicine as a tool for expanding access was explored as well as the special needs of vulnerable populations such as farmworkers. Participants learned how state and federal regulation and payment policies and state budget deficits impact the viability of rural health systems.

Nucleic Acid Sequencing Using Charge-Switch Nucleotides, John G. K. Williams, Gregory R. Bashford

Biomedical Imaging and Biosignal Analysis Laboratory

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.

A Summary Of Florida’S Law Of Quarantine Of Persons And Public Health Law Reform Issues

Florida Public Health Review

No abstract provided.

The Reference-Dose Place Conditioning Procedure Yields A Graded Dose-Effect Function, Rick A. Bevins

Department of Psychology: Faculty Publications

A major criticism of the place conditioning procedure for studying conditioned drug reward is that it is relatively insensitive to large quantitative shifts in drug dose (i.e., dose effects are all or none). Experiment 1 demonstrated this lack of sensitivity using a wide range of intravenous (IV) cocaine doses (O.1, 0.3, 0.45, 0.6, 0.9, or 1.2 mg/kg). Rats had cocaine repeatedly paired with one distinct end compartment of a 3 compartment apparatus; vehicle was administered in the other end compartment. In a subsequent drug-free choice test, the 0.45 to 1.2 mg/kg doses of cocaine conditioned a place preference. The magnitude …

Healing Model Of Nursing Practice In Nursing Homes, Virginia C. Carlson

Theses and Graduate Projects

The project is a healing practice model designed to advocate for the certified nursing assistants and elders in nursing homes. The Healing Model of Nursing Practice in Nursing Homes was designed to reclaim the inherent "caring-healing" attributes of nursing that have been excluded from nursing and the health systems as posited by Watson's Theory of Human Caring. The model integrates Zen and Reiki into a non-invasive practice, which is a method of healing that has been promoted by nurse theorists Jean Watson and Martha Rogers. The model promotes wholeness. The goal is to move our actions beyond regulatory processes to …

Automated Migration Analysis Based On Cell Texture: Method & Reliability, Jianfeng Qin, Thomas W. Chittenden, Ling Gao, Justin D D. Pearlman

Dartmouth Scholarship

Background: In this paper, we present and validate a way to measure automatically the extent of cell migration based on automated examination of a series of digital photographs. It was designed specifically to identify the impact of Second Hand Smoke (SHS) on endothelial cell migration but has broader applications. The analysis has two stages: (1) preprocessing of image texture, and (2) migration analysis.

Results: The output is a graphic overlay that indicates the front lines of cell migration superimposed on each original image, with automated reporting of the distance traversed vs. time. Expert preference compares to manual placement of leading …

Medical Education Digest, Vol. 7 No. 2 (March/April 2005), Nova Southeastern University

Medical Education Digest

No abstract provided.

An Inquiry Based Instructional Planning Model That Accommodates Student Diversity, Cheryl M. Jorgensen

Institute on Disability

The students in today’s public school classrooms represent great diversity and the struggle of teachers to teach all their students well. This paper describes an inquiry based instructional planning model that reflects lessons from the literature on effective teaching for diverse classrooms. An example of a high school lesson exemplifies the model. The model includes a framework for planning supports for students with extraordinary learning challenges.

Acute Toxicity Testing Without Animals: More Scientific And Less Of A Gamble, Gillian R. Langley

Application of Alternative Methods Collection

In this report, we argue specifically that acute toxicity data should not be sought from animal tests. The underlying principle of such tests on rats and mice is that the results can be effectively extrapolated to humans. In fact, after nearly 80 years of use of these tests, the predictivity of rodent data for human acute toxic effects has been disputed but never proven.

Variability In Lipid Profile Before And After Coronary Artery Bypass Grafting (Cabg), M Perwaiz Iqbal, Hasanat Mohammad Sharif, Naseema Mehboobali

Department of Biological & Biomedical Sciences

Objective: To investigate changes in total cholesterol, low density lipoprotein (LDL) cholesterol, high density lipoprotein (HDL) cholesterol, and triglycerides in serum of Pakistani patients before, immediately after and 5 days post CABG.
Method: Serum samples from 31 consecutive Pakistani angina patients undergoing CABG at the Aga Khan University Hospital were analyzed for total cholesterol, LDL cholesterol, HDL cholesterol and triglycerides using kit methods.
Results: Immediately after CABG, there is a significant decline in the mean levels of serum cholesterol, LDL cholesterol, HDL cholesterol and triglycerides. However, 5 days post CABG, there is a significant increase in the concentrations of total …

Jefferson Alumni Bulletin – Volume 54, Number 2, March 2005

The Bulletin (formerly the Jefferson Medical College Alumni Bulletin)

Jefferson Alumni Bulletin – Volume 54, Number 2, March 2005 Using Shaped Beam Surgery to Sculpt Therapy for Hard-to-Reach Brain Tumors; Page 8 Converting Adult Human Stem Cells to Longer-Lasting Dopamine Neurons; Page 9 New Evidence to Explain Statins’ Effects in Alzheimer’s Disease; Page 9 Liver Transplant: Less Rejection, Better Survival with New Drug Regimen; Page 10 Translational Research: Gene Therapy Rescues Failing Hearts in Lab Animals; Page 10 A Potential Trigger of Diabetic Kidney Disease; Page 11 Zinc May Help Prevent Esophageal, Oral Cancers; Page 11 Clinical Skills Center Named for Dr. and Mrs. Robert D. Rector ’48; Page …

Olfactory Neuroblastoma: A Case Report And Review Of The Literature, Shehzad Ghaffar, Iftikhar Salahuddin

Section of Otolaryngology, Head & Neck Surgery

Malignant tumors of the nasal cavity are rare. We report the case of an elderly woman who consulted us with a 4-year history of progressive nasal obstruction, occasional epistaxis, facial pain, and watering of the eyes. A diagnosis of olfactory neuroblastoma was established by histopathology and confirmed by immunohistochemistry. On staging, the mass was classified as a Kadish stage B tumor. The mass was excised via a lateral rhinotomy approach, and the tumor was peeled away completely from the cribriform plate with endoscopes. The patient underwent postoperative radiation, and she was free of recurrence at follow-up 15 months later.