Digital Commons Network^™

Visualization Of Lysosomal Membrane Proteins By Cryo Electron Tomography, Bridget M Mcveigh, José J De Jesús-Pérez, Dirk H Siepe, Prerana Gogoi, Shrawan Kumar Mageswaran, Marian Kalocsay, Elaine M Mihelc, Vera Y Moiseenkova-Bell

Faculty, Staff and Student Publications

Lysosomes are essential organelles for cellular homeostasis and signaling, with dysfunction linked to neurological disorders, lysosomal storage diseases, and cancer. While proteomics has advanced our understanding of lysosomal composition, the structural characterization of lysosomal membrane proteins in their native environment remains a significant challenge. Here, we developed a cryo electron tomography workflow to visualize lysosomal membrane proteins within intact, native lysosomal membranes. We isolated endolysosomes by independently targeting two lysosomal membrane proteins, transient receptor potential mucolipin 1 and transmembrane protein 192, enriching organelles that exhibited the expected morphology and proteomic composition of the endolysosomal system. Sub-tomogram averaging enabled the structural …

A Pooled Sample Study Of Opioid Use Disorder Treatment Wait Time Among A Pregnant Population In New York, Stanley Nkemjika, Gulshan Begum, Bolaji Yoade, Vaibhav Vyas, Henry Onyeaka, Olubusola Olatunji, Olaniyi Olayinka, Ayana Jordan

Department of Psychiatry and Human Behavior Faculty Papers

Background and Aim: Opioid use disorder (OUD) during pregnancy has become a major public health issue, with its prevalence rising significantly in recent years. The incidence of neonatal abstinence syndrome (NAS) has also surged, from 1.5 cases per 1000 hospital births in 1999 to 6.0 cases per 1000 in 2013. This study aims to identify and analyze the concerns faced by pregnant people in accessing OUD treatment on time, specifically focusing on New York. Methods: The pooled sample of 225,275 individuals represents pregnant patients with OUD who received treatment at substance use disorder (SUD) facilities across New York State between …

Genetic Determinants And Genomic Consequences Of Non-Leukemogenic Somatic Point Mutations, Joshua S Weinstock, Sharjeel A Chaudhry, Maria Ioannou, Maria Viskadourou, Paula Reventun, Yasminka A Jakubek, L Alexander Liggett, Cecelia Laurie, Jai G Broome, Alyna Khan, Kent D Taylor, Xiuqing Guo, Patricia A Peyser, Eric Boerwinkle, Nathalie Chami, Eimear E Kenny, Ruth J Loos, Bruce M Psaty, Russell P Tracy, Jennifer A Brody, Jeong H Yun, Michael H Cho, Ramachandran S Vasan, Sharon L Kardia, Jennifer A Smith, Laura M Raffield, Aurelian Bidulescu, Emily C O'Brien, Mariza De Andrade, Jerome I Rotter, Stephen S Rich, Russell P Tracy, Yii Der Ida Chen, C Charles Gu, Chao A Hsiung, Charles Kooperberg, Bernhard Haring, Rami Nassir, Rasika Mathias, Alex Reiner, Vijay G Sankaran, Charles J Lowenstein, Thomas W Blackwell, Goncalo R Abecasis, Albert V Smith, Hyun M Kang, Pradeep Natarajan, Siddhartha Jaiswal, Alexander Bick, Wendy S Post, Paul Scheet, Paul Auer, Theodoros Karantanos, Alexis Battle, Marios Arvanitis

Faculty, Staff and Students Publications

Clonal hematopoiesis (CH) is defined by the expansion of a lineage of genetically identical cells in blood. Genetic lesions that confer a fitness advantage, such as leukemogenic point mutations or mosaic chromosomal alterations (mCAs), are frequent mediators of CH. However, recent analyses of both single cell-derived colonies of hematopoietic cells and population sequencing cohorts have revealed CH frequently occurs in the absence of known driver genetic lesions. To characterize CH without known driver genetic lesions, we use 51,399 deeply sequenced whole genomes from the NHLBI TOPMed sequencing initiative to perform simultaneous germline and somatic mutation analyses among individuals without leukemogenic …

Type I Hybrid Effectiveness-Implementation Randomised Controlled Trial To Address Intergenerational Impact Of War Trauma And Resilience Among Second-Generation Refugee Children In The Usa: Resettled Refugee Families For Healing (Rrf4h) Study Protocol, Nhial T Tutlam, Tewodros W Liyew, Theresa S Betancourt, Byron J Powell, Shenyang Guo, Mary Mckay, Fred M Ssewamala

2020-Current year OA Pubs

INTRODUCTION: Children from refugee families resettled in the USA face higher risks of serious mental health challenges compared with their native-born peers. Research shows that refugee youth in high-income countries frequently suffer from trauma-associated disorders such as post-traumatic stress disorder (PTSD), depression and anxiety. The high prevalence of trauma-associated mental health problems among these youth may be attributed to their own trauma exposure, especially if born in conflict zones, and post-resettlement challenges like poverty, acculturation difficulties, racism and discrimination. However, they may also suffer from the effects of intergenerational trauma, where parental war trauma impacts them. This study aims to …

Design Of A Snp Panel For Highly Accurate Paternity Identification In Eşme Sheep, Onur Yilmaz, İbrahi̇m Cemal, Orhan Karaca, Cengi̇z Elmaci, Nezi̇h Ata

Turkish Journal of Veterinary & Animal Sciences

SNP paternity testing panels offer high-resolution capabilities for determining livestock paternity, thereby enhancing the reliability of genetic verification and ensuring pedigree record accuracy. In this study, we aimed to develop a SNP-based paternity test panel utilizing a 50K SNP chip for paternity testing and pedigree verification in the Eşme sheep breed. Additionally, the study sought to validate the paternity test panel. Based on data from 26 rams and 524 lambs of the Eşme sheep breed, the results indicated that the 50K SNP chip used demonstrated a very high genotyping rate, with the majority of its 53,738 SNPs exhibiting significant polymorphism. …

Bacterial Identification, Antibiotic Susceptibility Testing (Ast), And Epstein-Barr Virus (Ebv) Testing To Increase Organ Transplantation Within A Western Regional Community, Vedarrius D. Williams

Doctoral Dissertations and Projects

Clinical transplantation demands have increased immensely in recent years and, along with increasing supply shortages, many barriers to successful organ donation arise from a lack of dedicated testing to rule out microbial or bloodborne infections. There are currently minimal, federal regulatory requirements for transplantation specimen testing. The purpose of this study is to explore how to increase the viability and success of transplantations in the Western regions of the United States using kidney, pancreas, and kidney/pancreas transplant data from University Medical Center Southern Nevada (UMCSN) transplant center. UMCSN transplant center and Donor Services Laboratory (DSL) in Las Vegas perform bacterial …

Equal Survival For Black Americans With Multiple Myeloma When Appropriately Matched To White Americans, David E Mery, Guido Tricot, Samer Al Hadidi, Yihao Zhan, Cody Ashby, Clyde Bailey, Eric R Siegel, Daisy V Alapat, Hongwei Xu, Sandra Mattox, Caroline Schinke, Maurizio Zangari, Sharmilan Thanendrarajan, Qing Yi, Robert Z Orlowski, Frits Van Rhee, John D Shaughnessy, Fenghuang Zhan

Faculty, Staff and Student Publications

No abstract provided.

A Pooled Sample Study Of Opioid Use Disorder Treatment Wait Time Among A Pregnant Population In New York, Stanley Nkemjika, Gulshan Begum, Bolaji Yoade, Vaibhav Vyas, Henry Onyeaka, Olubusola Olatunji, Olaniyi Olayinka, Ayana Jordan

Faculty, Staff and Student Publications

Background and Aim: Opioid use disorder (OUD) during pregnancy has become a major public health issue, with its prevalence rising significantly in recent years. The incidence of neonatal abstinence syndrome (NAS) has also surged, from 1.5 cases per 1000 hospital births in 1999 to 6.0 cases per 1000 in 2013. This study aims to identify and analyze the concerns faced by pregnant people in accessing OUD treatment on time, specifically focusing on New York.

Methods: The pooled sample of 225,275 individuals represents pregnant patients with OUD who received treatment at substance use disorder (SUD) facilities across New York State between …

Pilot Study: Integrating The Epic Stroke Narrator Into Inpatient Code Stroke Protocols To Improve Symptom Onset Identification And Reduce Intervention Times, Stacy L. Carter

Doctoral Dissertations and Projects

In-hospital strokes are often underrecognized, leading to treatment delays and poorer outcomes compared to community-onset strokes. This scholarly project evaluated whether integrating the Epic Stroke Narrator into inpatient code stroke protocols could improve documentation of last known well time (LKWT) and reduce time to intervention. A 6-week quasi-experimental pilot study guided by the Iowa Model of Evidence-Based Practice and Rogers’ diffusion of innovations theory was conducted on a neuroscience telemetry step-down unit at a comprehensive stroke center in Richmond, Virginia. Education on the Stroke Narrator was provided to bedside nurses and was followed by implementation and evaluation of narrator use …

Empowering Psychiatric Nurse Practitioners To Address Nutritional Deficits: An Educational Quality Improvement Study, Beatrice Joan Richards

Doctoral Dissertations and Projects

Psychiatry is a complex and multifaceted discipline, and treatment approaches often vary based on provider preference and philosophy. Some focus heavily on pharmacotherapies, while others use psychotherapeutic methods, and still others take a more holistic approach involving full-body wellness. All three strategies are beneficial, and providing psychiatric care with a combination of them all can address the full spectrum of factors that influence one’s mental health. Much research, education, and clinical focus have been put on pharmacotherapy and psychotherapeutics. However, there is a marked lack of understanding regarding the holistic realm of psychiatric care, specifically the role of nutrition, amongst …

Clinical Management Of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome As A Curriculum Thread To Meet Accreditation Standards In Physical Therapy Education, Sallie W. Rediske, Heidi Shearin, Todd E. Davenport, Melinda W. Maxwell

Internet Journal of Allied Health Sciences and Practice

Purpose: ME/CFS is a debilitating, complex, chronic illness that is estimated to affect up to nine million Americans. It requires a paradigm shift in treatment approach to one of energy conservation due to metabolic effects resulting in exercise and activity intolerance. Including clinical management of ME/CFS as a curriculum thread in Doctor of Physical Therapy (DPT) programs is vital for safe and effective care of people with ME/CFS and can help meet accreditation standards. Methods: ME/CFS significantly impacts quality of life. It has been recognized by the World Health Organization since 1969 as a biomedical neurological disorder. Prior to …

Assessing Student Physical Therapists' Metacognition In A Foundational Course In An Accelerated Curriculum, Dalila Dias, Cheryl J. Hill, Annie Burke-Doe

Internet Journal of Allied Health Sciences and Practice

Introduction: This study addressed the problem of limited empirical evidence on the effective assessment and implementation of metacognitive strategies in accelerated health programs. Certainty-based markings have been implemented in health education to cultivate students’ self-monitoring and regulating skills; however, they have not been evaluated in a foundational course in an accelerated healthcare program. The theoretical framework that grounded this study was the metacognitive theory. Methods: A systematic review of the literature was performed by searching ProQuest, PubMed, EBSCO, and CINAHL databases Using a one-group pre-posttest study design, the research questions explored whether certainty-based markings improved student physical therapists’ test performance, …

Developing Empathy In Paramedic Students Using Didactic And Deliberate Practice Education Programs: A Mixed-Methods Study, Nick J. Waltrich, Jade Sheen Phd, Mathew Ling, Melissa O'Shea

Internet Journal of Allied Health Sciences and Practice

Purpose: Paramedics often attend to patients presenting with a mental illness, however research suggests paramedics have difficulties when attending to this patient cohort. Conversely, clinical psychologists receive training that focuses on developing strong therapeutic relationships with their patients through the use of empathy. As research has highlighted that paramedic students are known to demonstrate low levels of empathy, this study aimed to investigate the utility of two education methods used in clinical psychology training, didactic teaching and deliberate practice (DP), to develop the skill of empathy in paramedic students. Method: Students currently enrolled in an Australian paramedic university degree were …

An Overview Of The Health And Fitness Benefits From Sauna Exposure, Nicholas Smith, Ashley Wilson, Danica Buse, Alessandra Mondelli, Steven Vish, Morey J. Kolber

Internet Journal of Allied Health Sciences and Practice

This review provides an overview of the health and fitness benefits attributed to sauna exposure. The specific focus explores muscle performance and flexibility, heat acclimation for performance, cardiovascular and respiratory health parameters, all-cause mortality, endocrine response, inflammatory modulators, and exercise recovery. The overarching mechanisms underpinning the health and fitness benefits attributed to sauna use stem from hyperthermia, which activates thermoregulatory cardiovascular, neuroendocrine, and cytoprotective responses that promote homeostasis and adaptation to stress. Muscle performance improvements are attributed to plasma volume expansion, enhanced thermoregulatory efficiency, and upregulation of heat shock proteins. These adaptations enhance muscle contractility, strength, and rate of force …

An Authentic Leader Never Dies: Lessons Learned From The Seminal Work Of Elizabeth Yerxa, Daniel Cezar Da Cruz

The Open Journal of Occupational Therapy

This Opinion in the Profession is based on the enduring legacy of Elizabeth June Yerxa, a pioneering leader in occupational therapy and occupational science, following her passing in December 2024. Rather than a traditional "in memoriam," the author uses the present tense to emphasize Yerxa's ongoing influence. The paper highlights four key themes from Yerxa’s work: the commitment of occupational therapy to social change, the importance of an occupation-centered education, the term “engaging in occupation”, and the establishment of occupational science as a foundational discipline. Yerxa’s advocacy for critical thinking, holistic perspectives, and the integration of diverse fields …

Promoting Sense Of Belonging Among First-Generation Students In Occupational Therapy, Pola Ham

The Open Journal of Occupational Therapy

First-generation students in occupational therapy programs face unique challenges that can impact their sense of belonging and academic success, potentially hindering efforts to diversify the profession. There is limited data on occupational therapy students who identify as first-generation students. However, the literature has noted improved efforts toward a sense of belonging in higher education among students leads to feeling a sense of connectedness with their peers and faculty, aiding in overall well-being, academic performance and retention. In contrast, a lack of these efforts can lead students to experience a diminished sense of belonging to the institution. This article examines the …

Improving Relationship-Based Feeding Of Infants And Young Children With Disabilities: Developing A Guideline For Practice, Patricia Henton, Tsu-Hsin Howe

The Open Journal of Occupational Therapy

Feeding is a significant co-occupation of young children and their caregivers, unfolding in a social context. While addressing feeding challenges comprehensively because multiple contributing factors, the social-emotional dimension is often overlooked. This paper proposes a relationship-based feeding guideline to help occupational therapists incorporate this critical component. Centered on the child-caregiver dyad, the guideline prioritizes supporting the developmental, social-emotional, and environmental needs of the child. The guideline employs a biopsycho-ecological framework and family-centered approach, emphasizing caregiver training for promoting positive interaction. Actively involving caregivers and employing caregiver-child coaching facilitates the learning of developmentally appropriate feeding strategies that are then applied at …

Integrative Neural Networks Frame Of Reference: Application In Practice, Glenda E. Hux, Sarah Schoen

The Open Journal of Occupational Therapy

The INN Frame of Reference supports occupational engagement in caregivers of children and youth with complex health care needs. It guides the occupational therapist from evaluation to intervention providing a structure to identify challenges in occupational engagement. It describes the process of selecting evidence-based interventions to mitigate the impact of prolonged stress on the “whole” parent across contexts. The theoretical base outlines the mechanisms of action that support physiological, affective/cognitive, and social/spiritual health, and the concepts related to intervention. The completion of an occupational profile and the INN Evaluation Checklist sets the stage for data-driven decision making. The occupational therapist …

Integrative Neural Networks: A Novel Frame Of Reference To Support Occupational Engagement In Caregivers, Glenda E. Hux, Sarah A. Schoen

The Open Journal of Occupational Therapy

The Integrative Neural Networks (INN) frame of reference is an integrated approach to support occupational engagement in parents and primary caregivers of children and youth with complex health care needs (CYSHCN) guided by principles of family-centered care. Given the potential influence of prolonged, cumulative, and or repeated stress on physiological, affective/cognitive, and social/spiritual health, it is imperative that occupational therapists partner with parents and primary caregivers to positively impact occupational engagement across contexts. Further, this frame of reference provides an avenue to identify potential health and occupational disparities historically under-recognized as independent from the needs of the child. Based on …

Effects Of Yoga On The Mental Health Of Women With Gynecological Cancers: A Systematic Literature Review, Sabina Khan, Christianah Edema

The Open Journal of Occupational Therapy

Background: The diagnosis and treatment of gynecological cancers have substantial psychological impacts. Occupational therapists use relaxation and mindfulness techniques to manage pain, distress, and other mental health symptoms associated with cancer. This review aimed to evaluate the impact of yoga on the mental health and quality of life of women diagnosed with gynecological cancers.

Methods: A systematic search of five databases was conducted from their inception to the present. Grey literature was searched via Google Scholar and relevant conference proceedings. Eligible studies reported on the effect of yoga on mental health symptoms in women with gynecological cancers. A …

Uncovering Occupational Therapy’S Role In Addressing Postpartum Anxiety: A Scoping Review, Felicia Markfield, Carlin D. Reaume

The Open Journal of Occupational Therapy

Background: Occupational therapists are qualified to provide needed support for individuals experiencing postpartum anxiety. Postpartum depression is a more well known and researched disorder; however, postpartum anxiety is theorized to be more prevalent and less discussed (Nakić et al., 2018; Zappas et al., 2021). The objective of this scoping review is to find the themes in the literature highlighting the unique settings, assessments, and interventions used to support individuals with postpartum anxiety.

Methods: A literature search was conducted from Scopus, Sage Journals, OTSeeker, Science Direct, and EBSCO databases. Twenty articles were found and selected for review.

Results: …

Evaluation Of Receptiveness And Barriers To Including Occupational Therapists On Primary Care Teams, Jacquelyn P. Nash, Sue Dahl-Popolizio, Dayna Maureen Maniccia, Debra Collette

The Open Journal of Occupational Therapy

Background: Although primary care providers (PCP) and occupational therapists recognize occupational therapists can be on the primary care (PC) team to address lifestyle management related to chronic conditions, health literacy, and behavioral health, few occupational therapists work in this setting; PC remains an emerging practice area.

Method: We sought to determine if receptiveness to adding occupational therapists to PC teams has changed. We modified surveys from a 2017 study and added interviews to answer the following questions regarding occupational therapists in PC: (1) What are PCPs' and occupational therapists’ perceptions? (2) What are PCPs' comfort levels in delegating patient appointments …

Adopting Stuart Hall’S “Race, The Floating Signifier” As A Conceptual Framework Within Aquatic Research And Education, Kurt J. Cordice

International Journal of Aquatic Research and Education

During his 1997 lecture at Goldsmiths College in London, Stuart Hall, renowned for his contributions to sociology and cultural studies, offered his argument that the concept of race functions as a Floating Signifier. In this essay, Stuart Hall’s Race, the Floating Signifier is presented as a strong conceptual framework to support more accurate and effective positioning of race as an important consideration within aquatic research and education. Evidence from a range of disciplines, including historical research, is presented to establish both the importance and challenge of race within aquatic research and education. This evidence is also used to demonstrate …

Hydralazine Inhibits Cysteamine Dioxygenase To Treat Preeclampsia And Senesce Glioblastoma, Kyosuke Shishikura, Jiasong Li, Yiming Chen, Nate R. Mcknight, Thomas P. Keeley, Katelyn A. Bustin, Eric W. Barr, Snehil R. Chilkamari, Mahaa Ayub, Sun Woo Kim, Zongtao Lin, Ren-Ming Hu, Kelly Hicks, Xie Wang, Donald M. O'Rourke, J. Martin Bollinger, Zev A. Binder, William H. Parsons, Kirill A. Martemyanov, Aimin Liu, Megan L. Matthews

SKMC Student Presentations and Publications

Hydralazine (HYZ), a treatment for preeclampsia and hypertensive crisis, is listed by the World Health Organization as an essential medicine. Its mode of action has remained unknown through its seven decades of clinical use. Here, we identify 2-aminoethanethiol dioxygenase (ADO), a key mediator of targeted protein degradation, as a selective HYZ target. The drug chelates ADO's metallocofactor and can alkylate one of its ligands. The resultant inactivation stabilizes regulators of G protein signaling (RGS4 and RGS5) that ADO normally marks for proteolysis, explaining the drug's vasodilatory activity and comporting with observations of diminished RGS levels in both clinical preeclampsia and …

Resilience And Vulnerabilities Of Tumor Cells Under Purine Shortage Stress, Jianpeng Yu, Chen Jin, Cheng Su, David Moon, Michael A Sun, Hong Zhang, Xue Jiang, Fan Zhang, Nomi Tserentsoodol, Michelle L Bowie, Christopher J Pirozzi, Daniel J George, Robert Wild, Xia Gao, David M Ashley, Yiping He, Jiaoti Huang

Faculty, Staff and Students Publications

Purpose: Purine metabolism is a promising therapeutic target in cancer; however, how cancer cells respond to purine shortage, particularly their adaptation and vulnerabilities, remains unclear.

Experimental design: Using the recently developed purine shortage-inducing prodrug DRP-104 and genetic approaches, we investigated the responses in prostate, lung, and glioma cancer models.

Results: We demonstrate that when de novo purine biosynthesis is compromised, cancer cells employ microtubules to assemble purinosomes, multiprotein complexes of de novo purine biosynthesis enzymes that enhance purine biosynthesis efficiency. Although this process enables tumor cells to adapt to purine shortage stress, it also renders them more susceptible to the …

Differential Bk Channel Potentiation By Vanzacaftor Enantiomers Enables Therapy For Modulator-Ineligible People With Cystic Fibrosis, Nathalie Baumlin, Sumedha Gunewardena, Scott H Randell, Frank T Horrigan, Matthias Salathe

Faculty, Staff and Students Publications

No abstract provided.

Stereoelectroencephalography Reveals Neural Signatures Of Multisensory Integration In The Human Superior Temporal Sulcus During Audiovisual Speech Perception, Yue Zhang, John F Magnotti, Xiang Zhang, Zhengjia Wang, Yingjia Yu, Kathryn A Davis, Sameer A Sheth, H Isaac Chen, Daniel Yoshor, Michael S Beauchamp

Faculty, Staff and Students Publications

Human speech perception is multisensory, integrating auditory information from the talker's voice with visual information from the talker's face. BOLD fMRI studies have implicated the superior temporal gyrus (STG) in processing auditory speech and the superior temporal sulcus (STS) in integrating auditory and visual speech, but as an indirect hemodynamic measure, fMRI is limited in its ability to track the rapid neural computations underlying speech perception. Using stereoelectroencephalography (sEEG) electrodes, we directly recorded from the STG and STS in 42 epilepsy patients (25F, 17M). Participants identified single words presented in auditory, visual, and audiovisual formats with and without added auditory …

Sub-Ciliary Localization Of Cep290 And Effects Of Its Loss In Mouse Photoreceptors During Development, Abigail R. Moye, Michael A. Robichaux, Melina A. Agosto, Alexandre P. Moulin, Alexandra Graff-Meyer, Carlo Rivolta, Theodore G. Wensel

Faculty, Staff and Students Publications

No abstract provided.

Alterations Of The Upper Respiratory Microbiome Among Children Living With Hiv Infection In Botswana, Sweta M Patel, John Farirai, Mohamed Z Patel, Sifelane Boiditswe, Leabaneng Tawe, Shimane Lekalake, Mogomotsi Matshaba, Andrew P Steenhoff, Tonya Arscott-Mills, Kristen A Feemster, Samir S Shah, Nathan Thielman, Coleen K Cunningham, Lawrence A David, David M Murdoch, Matthew S Kelly

Faculty, Staff and Students Publications

Children living with HIV (CLWH) are at high risk of colonization and infection by respiratory pathogens, though this risk can be reduced by other microbes in the upper respiratory microbiome. The impact of HIV infection on the pediatric upper respiratory microbiome is poorly understood, and we sought to address this knowledge gap by identifying associations between HIV infection and the nasopharyngeal microbiomes of Batswana children. We enrolled Batswana CLWH (< 5 years) and age- and sex-matched HIV-exposed, uninfected and HIV-unexposed, uninfected children in a cross-sectional study. We used shotgun metagenomic sequencing to compare nasopharyngeal microbiomes by HIV status. Among the 143 children in this study, HIV and HIV-associated immunosuppression were associated with alterations in nasopharyngeal microbiome composition, including lower abundances of Corynebacterium species associated with resistance to bacterial pathogen colonization. These findings suggest that the upper respiratory microbiome may contribute to the high risk of respiratory infections among CLWH.

Estrogen-Related Receptor Alpha Promotes Skeletal Muscle Regeneration And Mitigates Muscular Dystrophy, Thi Thu Hao Nguyen, Ya Xiang Huang, Svitlana Poliakova, Citu Citu, Eira Mann, Danesh H Sopariwala, Zhongming Zhao, Ashok Kumar, Vihang A Narkar

The Brown Foundation: Institute of Molecular Medicine

Skeletal muscle regeneration in chronic muscle diseases such as Duchenne Muscular Dystrophy (DMD) has remained clinically unsurmountable. Estrogen‐related receptor alpha (ERRα) plays a critical role in adult skeletal muscle metabolism and exercise fitness. Whether ERRα activation can drive muscle regeneration and mitigate dystrophy in DMD is not known. We have investigated ERRα signaling in pre‐clinical models of acute muscle injury and DMD. ERRα is induced in differentiating C2C12 myoblast and regenerating muscle. ERRα silencing suppressed proliferation and differentiation in C2C12 myoblasts. RNA sequencing revealed that angiogenic factor and proliferation genes were downregulated by ERRα knockdown in proliferating cells, whereas oxidative …