Digital Commons Network^™

Development Of Tunable Rose Bengal Based-Nanogumbos As Potential Selective Chemotherapeutic Agents, William J.A. Russell

Honors College Theses

As cancer remains one of the greatest threats to the global population, the development of chemotherapeutic agents that are selective and tunable is of critical importance. Herein, a series of Rose Bengal (RB)-based GUMBOS, a group of uniform materials based on organic salts, were synthesized via counterion exchange between [Na]₂[RB] and three organic cations: tetrabutylphosphonium bromide [TBP][Br], tetraphenylphosphonium chloride [TPP][Cl], and 1-dodecyl-3-methylimidazolium chloride [C₁₂MIm][Cl]. The resulting GUMBOS were characterized through FT-IR, ESI-MS, NMR, and UV-Vis Spectroscopy. Partition coefficient studies indicated all GUMBOS exhibited hydrophobic physiochemical characteristics. These hydrophobic RB-based GUMBOS …

Optimizing Healthy Aging: A Preventative Approach To Support Community-Dwelling Older Adults, Jada Welke

Occupational Therapy Capstones

Introduction: Healthy aging is a complex and multidimensional process influenced by physical, cognitive, psychosocial, environmental, and lifestyle factors across the lifespan. Increasing evidence from researchers suggests that many health care systems continue to rely on reactive models that address decline only after functional limitations emerge. Preventive interventions initiated in midlife have the potential to significantly influence long-term health outcomes, independence, well-being, and quality of life. As a result, there is growing recognition that multidimensional, proactive screening and intervention should begin earlier, before age-related conditions become clinically significant.

Purpose: The purpose of this scholarly project was to address this need through …

Achieving Successful Occupational Performance During Transitions In Eating Disorder Treatment, Elise Kay Ramberg

Occupational Therapy Capstones

Introduction: Individuals with eating disorders experience challenges in occupational engagement that stem from contributing meaning, value, and purpose to occupations that support eating disorder behaviors rather than promote quality of life (Mack et al., 2023). Recovery is highly dependent on the transition between both levels of care and back into the patient’s natural environment. Occupational therapists play an essential role in supporting patients as they transition and learn to re-engage in their lives outside of their eating disorder (Mack et al., 2023). The purpose of this project was to create materials for the occupational therapist to employ with eating disorder …

Cultivating Connections: Empowering Agricultural Community Members Through Collaborative Care, Kearyn E. Nelsen

Occupational Therapy Capstones

Background: Agriculture and mental health – two words not typically used in the same sentence; however, considering the rate of suicide among those working in the agriculture profession is significantly higher than the general population, the need for both awareness and action is critically important. Despite a lack of research specific to occupational therapy and the agricultural population, occupational therapists on an interprofessional healthcare team are uniquely positioned to serve this population. Interprofessional collaboration is essential when serving agricultural community members, whose needs involve a complexity of physical, mental, social, and environmental factors, each impacting the overall health and well-being …

Beyond Survival: Empowering Mental Health Recovery After Acquired Brain Injury, Paige Mackenzie Kozak

Occupational Therapy Capstones

Purpose: Individuals with acquired brain injury (ABI) frequently experience psychosocial and cognitive challenges, including depression, anxiety, emotional dysregulation, and identity changes which can significantly impact rehabilitation outcomes and participation in daily occupations. Despite this, outpatient rehabilitation clinicians may feel underprepared to recognize and address these concerns within their scope of practice. The purpose of this scholarly project was to develop an educational onboarding program to support interdisciplinary rehabilitation staff in identifying and responding to psychosocial needs of individuals with ABI, while promoting trauma-informed and patient-centered care.

Methodology: A literature review was conducted to examine common psychosocial and cognitive outcomes following …

Holistic Occupational Therapy Approach In Addressing Women’S Health Needs & Access To Care, Lilja Jurva

Occupational Therapy Capstones

Background: As women across the lifespan experience complex physical, emotional, and occupational challenges related to maternal transitions, mental health needs, chronic conditions, and rural healthcare disparities, occupational therapy is uniquely equipped to address the complex challenges. Services have been proven to be directly correlated to lower incidence of preterm birth, cesarean delivery, low birth weight, excessive gestational weight gain, diabetes mellitus, and hypersensitive disorders, and higher incidence of vaginal delivery. A clear gap for maternal support services was identified in the Grand Forks community through literature review, interviews, and community engagement, leading to the development of NoDak Therapy and Wellness. …

Empowering Caregivers: An Occupational Therapy-Informed Educational Program For Dementia Care In Rural Communities, Maria Lynn Johnson

Occupational Therapy Capstones

Purpose: Hospitalization presents significant risks for individuals with dementia, including increased behavioral and psychological symptoms (BPSD), delirium, functional decline, and reduced occupational engagement (Boltz et al., 2023; Teichmann et al., 2019; Zwingmann et al., 2020). Acute care environments are often overstimulating, unfamiliar, and task-oriented, which can exacerbate cognitive impairment and contribute to caregiver distress. Despite strong evidence supporting nonpharmacological, occupation-based interventions, structured engagement opportunities are rarely integrated into acute care workflows (Burgdorf et al., 2023; Faeder et al., 2023). This doctoral capstone project aimed to develop occupation-based dementia activities for use during hospitalization while equipping formal and informal caregivers with …

Family-Centered Programming For A Rural Midwest Emergency Shelter: An Occupational Lens, Ariel Kathryn Tix

Occupational Therapy Capstones

Introduction: After a period of decline in homelessness, a startling trend reversal occurred between 2023 and 2024; there was a 39% increase in families experiencing homelessness in the United States (de Sousa & Henry, 2024). Rural homelessness is less visible and often looks different from urban homelessness, which presents challenges that require a different approach (Brott et al., 2019). Individuals experiencing homelessness have challenges in every area of occupation and could benefit from novel strategies and resources. Challenges include parenting, accessing medical care, obtaining permanent housing, community mobility, and meaningful routine development (American Occupational Therapy Association, 2020; Brott et al., …

Integrative Approaches And Data Analysis For Single-Cell Rna Sequencing Data, Teng Long

Computer Science and Engineering Dissertations

The rapid growth of single-cell RNA sequencing and transcriptomic datasets has created major computational challenges in causal discovery, representation learning, and biologically faithful data generation. To address these challenges, this dissertation presents three complementary deep learning frameworks for the analysis and modeling of transcriptomic data. Together, these methods form an integrative computational toolkit for understanding complex biological systems from high-dimensional and heterogeneous gene expression data.

First, this dissertation introduces DAG-VAERL, a causal discovery framework that integrates variational autoencoders, graph neural networks, reinforcement learning, and attention mechanisms to infer directed acyclic graphs for gene regulatory network analysis. DAG-VAERL improves causal structure …

Dental Implantology: A Literature Review, Kylie Taylor

Undergraduate Honors Theses

Dental implant therapy is the process of replacing teeth as a form of treatment to improve quality of life, function, and esthetics. The success of the implant depends on the surgical technique, type of prosthetic, and factors related to individual patients. The most important factor for implant success is osseointegration. Osseointegration is a process in which success is determined by the bone quality, the surface characteristics of the implant and the surgical approach. Some approaches to improve the osseointegration of the implant are low-speed drilling, guided surgery, and reduced surgical trauma at the site. The impact of systemic conditions, health …

Dose-Dependent Developmental Toxicity Of Cisplatin In Zebrafish Embryos, Reagan D. Sellers, Katherine T. Doucet, Jinhee Park, Andrea Florian

SPARK Symposium Presentations

Cisplatin is a widely used chemotherapy drug for treating various types of cancer. Although cisplatin is effective in killing cancer, it can also cause toxic side effects in normal tissues. Because cisplatin can cross the placental barrier, its use during pregnancy is generally avoided, particularly during early embryonic stages of fetal development. Exposure to cytotoxic drugs during embryogenesis may lead to developmental abnormalities, birth defects, or pregnancy complications. Understanding how cisplatin affects early development is therefore important.          
       Zebrafish (Danio rerio) are an established model for studying developmental toxicity due to their rapid external development, transparent embryos, and genetic similarity to …

Effects Of Glucagon And Insulin On Sw480 Colorectal Cancer Cells In Combination With Cisplatin, Sarah M. Gabous, Andrea Florian

SPARK Symposium Presentations

Colorectal cancer is known to be one of the most common forms of cancer in the world. Over time, treatment responses are affected by various metabolic changes along with chemotherapies. Glucagon signaling and Insulin are linked to colorectal cancer and have also been shown to affect tumor growth, as well as the chemotherapy drug response.In this experimental study, the purpose was to determine whether glucagon and insulin decrease the viability of SW480 colorectal cancer cells during cisplatin (CDDP) treatment. CRC cells were treated with cisplatin alone and in combination with either glucagon or insulin, and cell viability was measured to …

Altered Natural Killer Cell Function In Children With Severe Malaria, Grace Turyasingura, Kagan A. Mellencamp, Ruth Namazzi, Robert Opoka, Chandy C. John, Geoffrey T. Hart

Paediatrics and Child Health, East Africa

Memory-like natural killer (NK) cells with enhanced antibody-dependent cellular cytotoxicity (ADCC) have correlated with protection from uncomplicated malaria in prior studies. However, the role of NK cells in severe malaria (SM) has not been characterized. In Ugandan sites with moderate and low malaria transmission, we evaluated NK cell (CD56bright, CD56dim, CD56neg) phenotype and ADCC function by flow cytometry in children < 5 years of age with SM (n = 21) and control community children (CC, n = 19). Children with SM had similar total NK cell counts to CC. Children with SM had a higher proportion of LILRB1+ NK cells than CC. The level of malaria transmission …

Elevated Bmi And The Effect On Breastfeeding Duration In Postpartum Individuals: An Integrative Review, Abigail E. Mobilia, Kirstin A. Wyly

All Electronic Theses and Dissertations

Introduction: It has been well documented that exclusive breastfeeding provides benefits for infants and postpartum individuals, yet rates remain suboptimal in the United States, particularly among those with elevated pre-pregnancy body mass index (BMI). Evidence suggests that elevated BMI is associated with shorter breastfeeding duration and may be influenced by physiologic, social, and structural factors. This integrative review synthesizes existing literature to clarify the relationship between elevated BMI and exclusive breastfeeding duration among postpartum individuals.
Methods: An integrative review was conducted following Whittemore and Knafl’s (2005) framework and PRISMA 2020 guidelines. A systematic search of PubMed, CINAHL, and Nursing & …

Gestational Diabetes Mellitus And Its Psychological Impacts During Pregnancy And Postpartum, Nehemie B. Hypolite, Charline Fast

All Electronic Theses and Dissertations

Introduction: This review explores the link between gestational diabetes (GDM) and psychological outcomes. GDM affects 8–10% of pregnancies and is typically diagnosed at 24–28 weeks. This burden leads to anxiety, depression, and fear. Postpartum concerns often persist for mother and infant.

Methods: Whittemore and Knafl’s (2005) method was used to synthesize data. The research databases included CINAHL and PubMed. Covidence supported screening and PRISMA diagram development. Key search terms included “anxiety and GDM,” “depression and GDM,” “GDM and postpartum depression,” “gestational diabetes,” and “pregnancy.” Inclusion criteria included full-text articles published after 2010, original studies, patients undergoing diabetic treatment (medication and/or …

The Impact Of Patient-Centered Care On Women With Infertility And Pregnancy Loss: An Integrative Review, Kaidynce R. Sweazy

All Electronic Theses and Dissertations

Introduction: Infertility affects roughly 17.5% of adults worldwide, while pregnancy loss occurs in up to 30% of pregnancies. These reproductive traumas increase the risk of developing a mental health disorder, such as anxiety, depression, or post-traumatic stress disorder (PTSD). The purpose of this review is to identify the influence of patient-centered care on psychological outcomes and experiences of care in women with infertility and pregnancy loss.
Methods: A literature search of the PubMed and Cinahl databases was conducted for original studies published from January 2016 to January 2026 that address the psychological outcomes and/or experiences of women who experienced infertility …

Dim Light At Night Impacts Circadian Rhythms And Alzheimer’S Disease-Like Neuroinf Lammation And Neuropathology In Humanized App Saa Knock-In Mice, Marilyn J. Duncan, Margaret R. Hawkins, Leke Bytyqi, Haleigh R. Whitlock, Savannah M. Shepard, Makayla F. Cox, Esther G. Drinkard, Teresa Macheda, Kelly N. Roberts, Katharina Kohler, Mary-Claire Schmidt, Carrie E. Johnson, Sridhar Sunderam, Bruce F. O'Hara, Michael Paul Murphy, Adam D. Bachstetter

Neuroscience Faculty Publications

Artificial light at night (light pollution) is widespread but understudied in the context of Alzheimer’s disease (AD). Sleep and circadian disruption have been linked to amyloid-β (Aβ) accumulation and neuroinflammation, but whether dim light at night (dLAN) modifies these processes remains unclear. We tested whether chronic dLAN exposure (8 lux during the dark phase, 8 weeks) alters circadian rhythms, amyloid pathology, and neuroinflammation in 12–13 month-old humanized APP knock-in (KI) mice. hAPP^SAA KI mice, which develop plaques, were compared with hAPP^WT KI controls carrying only a humanized APP sequence. dLAN reduced circadian rhythm amplitude and stability while increasing …

Brain-Derived Extracellular Vesicles Potentially Mediate Crosstalk With Peripheral Organs In Neurodegenerative Diseases, Ramzi H. Hamdalla, Vibha B. Bhaskar, Changhai Tian

Toxicology and Cancer Biology Faculty Publications

Brain-Derived Extracellular vesicles (BDEVs) are emerging mediators of intra- and interorgan communication in neurodegenerative diseases (NDs) such as Alzheimer’s Disease (AD) and Parkinson’s Disease (PD). A growing body of evidence suggests that BDEVs play an important role in modulating intercellular communication within the central nervous system in the pathogenesis of many NDs. By transporting non-coding RNAs (e.g., miRNAs) and important pathological proteins, BDEVs also influence peripheral organs and contribute to the progression of disease in the central nervous system (CNS). This review extends the understanding of NDs beyond solely brain dysfunction and gives a novel framework for the progression of …

Traumatic Brain Injury Exacerbates Mitochondrial Dysfunction In App/Ps1 Knock-In Mice Through Time-Dependent Pathways, Elika Z. Moallem, Hemendra J. Vekaria, Teresa Macheda, Margaret R. Hawkins, Kelly N. Roberts, Samir P. Patel, Patrick G. Sullivan, Adam D. Bachstetter

Spinal Cord and Brain Injury Research Center Faculty Publications

Cerebral hypometabolism occurs in both traumatic brain injury (TBI) and Alzheimer's disease (AD), but whether these conditions act through distinct or overlapping mechanisms is unclear. TBI disrupts cerebral metabolism via blood–brain barrier damage, altered glucose transporter expression, calcium buffering abnormalities, and oxidative damage to metabolic enzymes. AD-related hypometabolism is linked to amyloid-β (Aβ) effects on mitochondria, including impaired respiration, oxidative stress, and altered mitophagy, fusion, and fission. We tested whether TBI-induced mitochondrial dysfunction exacerbates Aβ-mediated impairment using a closed-head injury (CHI) model in APP/PS1 knock-in (KI) mice. Injuries were delivered at 4–5 months of age, before plaque formation and mitochondrial …

Current Concepts In Hip And Core Assessment To Reduce The Risk Of Acl Injury, Jeremy M. Burnham, Anthony T. Drazick, Ghislain Aminake, Darren L. Johnson, Mary Lloyd Ireland, Brian W. Noehren

Orthopaedic Surgery and Sports Medicine Faculty Publications

Background: Hip and trunk (“core”) muscle dysfunction has emerged as a modifiable contributor to lower-limb biomechanics linked with non-contact anterior cruciate ligament (ACL) injuries. Clinicians increasingly target core stability and control during injury-prevention screening and post-operative rehabilitation programs; however, the literature lacks a focused synthesis of core-directed strategies for ACL injury mitigation. The purpose of this current concepts review is to synthesize current evidence on hip- and core-focused assessment and rehabilitation strategies that may mitigate ACL injury risk.

Design: Current Concepts Review.

Methods: A narrative search of PubMed and Google Scholar (January 2000 – April 2025) identified biomechanical, prospective, interventional, …

Candidate Interaction Partners Of Calpain-5 Suggest Clues To Its Involvement In Neovascular Inflammatory Vitreoretinopathy, Jozsef Gal, Vimala Bondada, Rachel Crasta, Dorothy E. Croall, Calvin P. Vary, James W. Geddes

Spinal Cord and Brain Injury Research Center Faculty Publications

Although calpain-5/CAPN5 is widely expressed in mammals, little is known regarding its functions. Pathogenic mutations of CAPN5 are causal for a devastating autoimmune eye disease, neovascular inflammatory vitreoretinopathy (NIV). To provide insight into both the physiological and pathological roles of CAPN5, it is essential to identify candidate interaction partners and possible substrates. Human SH-SY5Y neuroblastoma cells, transfected with full-length catalytically dead (Cys81Ala) CAPN5-3×FLAG, were used for anti-FLAG co-immunoprecipitation (co-IP) and quantitative proteomics using Sequential Window Acquisition of all THeoretical mass spectra (SWATH-MS). Fifty-one proteins were enriched at least four-fold, p < 0.01, relative to cells transfected with an empty FLAG vector. A high proportion (24/51) of candidate CAPN5 interaction partners are associated with protein quality control, including components of the chaperonin, chaperone, and ubiquitin–proteasome systems. Additional candidate interactors include tubulins, kinases, phosphatases, G proteins, and mitochondrial proteins. CAPN5 interactions for 14 of the candidate proteins were confirmed by co-IP and immunoblotting. Of these 14 proteins, 11 exhibited in vitro calcium-induced proteolysis following co-IP with WT CAPN5-3×FLAG. Impaired calcium-induced proteolysis of co-IP proteins was observed for the pathogenic CAPN5 variants R243L and R289W. Further studies are needed to validate the association of candidate CAPN5 interactors with proteins and complexes suggested by the SWATH-MS and co-IP results, and the possible role of CAPN5 within such complexes. The possible involvement of CAPN5 in protein quality control is relevant to NIV, as defects in protein quality control have been implicated in inherited retinal disorders. Proteomic data are available via ProteomeXchange with identifier PXD068008.

Difficult-To-Treat Rheumatoid Arthritis With Concomitant Yao Syndrome With Response To Dual-Inhibitor Therapy, Nawar Aljundi, Paramarajan Piranavan

Internal Medicine Faculty Publications

No abstract provided.

An Unbiased Molecular Characterization Of Peripartum Cardiomyopathy Hearts Identifies Mast Cell Chymase As A New Diagnostic Candidate, J. F. Mulvey, C. Sailer, J. S. Achter, Gregory Nathaniel Milburn, R. C. Bretherton, K. Kahnert, S. Erbil Bilir, H. Hvid, C. Pyke, F. Gustafsson, L. Adamo, Kenneth S. Campbell, K. M. Herum, A. Lundby

Internal Medicine Faculty Publications

Peripartum cardiomyopathy (PPCM) is a rare form of acute heart failure that develops in women toward the end of pregnancy or early postpartum. No effective, specific treatment for PPCM is available and heart transplantation or mechanical circulatory support may be required in severe cases where drug treatment for heart failure is insufficient. The mechanisms through which the disease progresses are not well understood, and despite similar clinical characteristics to dilated cardiomyopathy of other etiologies (nonperipartum cardiomyopathy; NPCM) it is not known how the molecular remodeling differs between these groups. We aimed to provide insight into the human PPCM heart using …

Anatomical Atlas Of Kinase Responsiveness To Weight Gain: Adipose Depot Reprogramming In Diet-Induced Adiposity, Wang-Hsin Lee, Zachary A. Kipp, Sally N. Pauss, Genesee J. Martinez, Mei Xu, Terry D. Hinds, Jr.

Pharmacology and Nutritional Sciences Faculty Publications

Background/Objectives: Adipose tissue depots located at different anatomical sites exert differential functions in response to adiposity and glucose intolerance. These fat depots exhibit distinct metabolic signaling patterns that may influence pathological fat accumulation, thereby affecting the efficacy of anti-obesity interventions. Nonetheless, the mechanisms underpinning depot-specific signaling and pathway responsiveness remain insufficiently understood.

Methods: Kinase activity was characterized during the progression of adiposity across five adipose tissue depots in obese versus lean mice using the advanced PamGene kinome technology. Furthermore, kinase pathways in human preadipocytes and mouse 3T3-L1 preadipocytes were analyzed and compared with those in their differentiated, mature adipocytes. The …

Social Engagement Moderates The Relationship Between Cognitive Functioning, Depressive Symptoms, And Restless Sleep In Older Black Adults, Sunkanmi Folorunsho, Olufunmilola Abraham, Darlingtina K. Esiaka

Pharmacy Practice and Science Faculty Publications

INTRODUCTION: Older Black adults face higher dementia risk, but it is unclear whether social engagement offsets the effects of depression and restless sleep.

METHODS: We analyzed 1,905 Black adults aged 50+ from the 2016-2020 Health and Retirement Study (HRS). Linear mixed-effects models tested how depressive symptoms, restless sleep, and social engagement predicted baseline cognition and cognitive decline.

RESULTS: Higher depressive symptoms and restless sleep were associated with lower baseline cognition (about 0.2 and 0.5 points lower) and faster decline (β×time ≈ −0.07 and −0.05). Greater social engagement predicted higher baseline cognition (about 0.6 points higher) and slower decline (β×time ≈ …

Developing Curcumin Analogues For Treating Breast Cancer, Tulasi Laxmi Nutalapati

Theses, Dissertations and Capstones

Breast cancer is still one of the most common types of cancer and one of the most common causes of cancer-related deaths in women around the world. Despite significant advances in early detection and treatment strategies, drug resistance, systemic toxicity, and poor outcomes in aggressive subtypes continue to limit the overall effectiveness of cancer therapy. Natural compounds have become promising anticancer agents because they can target many different things and are not very toxic. Curcumin has shown promise as an anticancer drug, but it cannot be used in the clinic because it does not absorb well, breaks down quickly, and …

Comparative Evaluation Of R134a And Hfo‐1234ze Cryogen Spray Cooling Using A Mouse Model With Controllable Epidermal Pigmentation, Wangcun Jia, Yurie Jeong, Crystal Huynh, Oswald Lai, Emily Nguyen, Grant Swajian, Jade Niu, Ayana Kashikar, Joshua Khaw, Hong Pu, Bernard Choi, John August D'Orazio, J. Stuart Nelson, Bahman Anvari

Pediatrics Faculty Publications

Objectives: Cryogen spray cooling (CSC) is critical for protecting the epidermis during laser dermatologic procedures. The widely used cryogen, R134a, has a high global warming potential (GWP), motivating interest in environmentally favorable alternatives such as HFO-1234ze. This study evaluated whether HFO-1234ze provides epidermal protection comparable to R134a during laser exposure and investigated the mechanistic basis underlying any differences in cooling performance.

Methods: Laser-induced epidermal injury in conjunction with two cryogens was assessed in K14-SCF Mc1r^e/e mice with pharmacologically controllable interfollicular epidermal pigmentation. Laser treatment sites were assigned fluences relative to baseline skin lightness (L*) to span subthreshold …

The Effects Of Childhood Maltreatment On Executive Functioning: The Difference Between Cumulative Trauma, Abuse Severity, And Neglect, Kelly Krehbiel

Master's Theses or Doctor of Nursing Practice

Childhood maltreatment is prevalent in the United States with around 558,899 children being victims of abuse in 2022 (U.S Department of Health & Human Services, 2024). Neglect is the most common form of abuse with around 74 percent of abused children being neglected; however, neglect tends to be the least researched form of abuse. Childhood maltreatment has long-term effects on a person’s mental, physical, and cognitive health. Previous research suggests that childhood maltreatment leads to severe deficits in executive functioning skills such as working memory and cognitive flexibility. Executive functioning skills are important for almost every aspect of life including …

Harmony In Healing: Addressing The Psychosocial Needs Of Individuals Living With Lymphedema, Bria K. Brombacher

Entry Level Occupational Therapy Doctoral Capstone Projects Class of 2026

Lymphedema is a chronic and incurable condition associated with significant physical and psychosocial challenges, including depression, anxiety, social isolation, and reduced quality of life. Despite increasing recognition of these concerns, limited interventions exist that specifically address the psychosocial needs of individuals living with lymphedema. Additionally, individuals within this population often report limited provider awareness of lymphedema within the healthcare system. The purpose of this doctoral capstone project, Harmony in Healing: Addressing the Psychosocial Needs of Individuals Living with Lymphedema, was to promote well-being, improve quality of life, and increase awareness of the psychosocial implications of lymphedema through occupational therapy intervention. …

Absolute Quantification And Identification Of Rna From Rna-Lipid Nanoparticles Using High Resolution Mass Spectrometry, Jason C. Funderburk, Yasir A. Alshehry, Matthew S. Halquist Phd, Sandro R.P. Da Rocha Phd

Graduate Research Posters

Background

RNA therapeutics are a rising drug category with potential use for a range of conditions encompassing infectious diseases to therapies for cancer, diseases, and genetic disorders. RNA-lipid nanoparticles (RNA-LNPs) are the prominent delivery method for these therapeutics approved products include mRNA vaccines and polyneuropathy treatments. The emergency use authorizations and orphan drug status of current RNA-LNP drugs has allowed approval without finalization of the regulatory analytical procedures for quality monitoring. The objective of the study was to develop an LC-MS assay to simultaneously measure identity and concentration of two therapeutically relevant intact RNA constructs extracted from RNA-LNPs to enhance …