Digital Commons Network^™

Use Of A Multiplex Transcript Method For Analysis Of Pseudomonas Aeruginosa Gene Expression Profiles In The Cystic Fibrosis Lung, Alex H. Gifford, Sven D. Willger, Emily L. Dolben, Lisa A. Moulton, Dana Dorman, Heather Bean, Jane Hill, Thomas Hampton, Alix Ashare, Deborah Hogan

Dartmouth Scholarship

The discovery of therapies that modulate Pseudomonas aeruginosa virulence or that can eradicate chronic P. aeruginosa lung infections associated with cystic fibrosis (CF) will be advanced by an improved understanding of P. aeruginosa behavior in vivo We demonstrate the use of multiplexed Nanostring technology to monitor relative abundances of P. aeruginosa transcripts across clinical isolates, in serial samples, and for the purposes of comparing microbial physiology in vitro and in vivo The expression of 75 transcripts encoded by genes implicated in CF lung disease was measured in a variety of P. aeruginosa strains as well as RNA serial sputum samples …

Continuous Care In Complex Contexts: Access To Health Services For Noncommunicable Diseases Among Syrian Refugee Women In Jordanian Host Communities, Jennifer Ostrowski

Independent Study Project (ISP) Collection

This study examines how, when and where Syrian refugee women living in a host community in central Jordan access health services related to noncommunicable diseases. Noncommunicable diseases are the leading cause of death and disability worldwide, but can be effectively managed through timely treatment. Examining access to care for these diseases in the context of humanitarian emergencies, such as war and displacement, is particularly important because they require continuous care, which may be interrupted during emergencies, and because they can cause acute complications, which may be exacerbated by emergencies (WHO, 2016).

Previous studies indicate a high burden of NCDs among …

A Systematic Review Of The Relationship Between Familism And Mental Health Outcomes In Latino Population, Esmeralds Valdivieso-Mora, Casie L. Peet, Mauricio Garnier-Villarreal, Monica Salazar-Villanea, David K. Johnson

College of Nursing Faculty Research and Publications

Background: Familismo or familism is a cultural value frequently seen in Hispanic cultures, in which a higher emphasis is placed on the family unit in terms of respect, support, obligation, and reference. Familism has been implicated as a protective factor against mental health problems and may foster the growth and development of children. This study aims at measuring the size of the relationship between familism and mental health outcomes of depression, suicide, substance abuse, internalizing, and externalizing behaviors. Methods: Thirty-nine studies were systematically reviewed to assess the relationship between familism and mental health outcomes. Data from the studies were comprised …

In Search Of Argentinidad: Identity Affirming Bodies In Movement In Latino-America, Melissa Maldonado-Salcedo

Dissertations, Theses, and Capstone Projects

This project is a multi-sited investigation into the production of Argentinidad (the embodied feeling of Argentine national identity) post the economic crisis of 2001 known as el Argentinazo. A special attention is paid to the role of the body as a culturally and socially mediated site of identity formation. Additionally, this project engages with the intersections of cultural and psychoanalytic theories that have influenced Argentinean self-identity in addition to social identities that are negotiated in moments of personal and national crisis. This project examines the roles and relationships of family and migration within Argentinean diasporic communities originating from the Provinces …

N-Acetylcysteine In Combination With Igf-1 Enhances Neuroprotection Against Proteasome Dysfunction-Induced Neurotoxicity In Sh-Sy5y Cells, Benxu Cheng, Pinki Anand, Anxiu Kuang, Feroz Akhtar, Virginia L. Scofield

School of Medicine Publications

Ubiquitin proteasome system (UPS) dysfunction has been implicated in the development of many neuronal disorders, including Parkinson’s disease (PD). Previous studies focused on individual neuroprotective agents and their respective abilities to prevent neurotoxicity following a variety of toxic insults. However, the effects of the antioxidant N-acetylcysteine (NAC) on proteasome impairment-induced apoptosis have not been well characterized in human neuronal cells. The aim of this study was to determine whether cotreatment of NAC and insulin-like growth factor-1 (IGF-1) efficiently protected against proteasome inhibitor-induced cytotoxicity in SH-SY5Y cells. Our results demonstrate that the proteasome inhibitor, MG132, initiates poly(ADP-ribose) polymerase (PARP) cleavage, caspase …

Why Consciousness Matters: Insights From A New Generation, Kathleen Noble

CONSCIOUSNESS: Ideas and Research for the Twenty-First Century

The University of Washington-Bothell is home to a transdisciplinary and integral minor in consciousness that is the first and only such program at a public research university. This study reports a content analysis of reflection essays submitted by 122 students who completed at least four of the five courses that comprise the minor between 2012 and 2016. Results indicate that students derived significant benefits from this approach to the study of consciousness, including a deep appreciation for the depth and breadth of the mind and the need to release their understanding of consciousness from the constraints of scientific materialism. They …

Cultural Competence Of Nurse Practitioners: Providing Care For Gay And Lesbian Clients, Paul S. Smith

Faculty Presentations

This plenary presentation given by Dr. Paul Smith was part of a panel on LGBTQ health in nursing. Smith identifies and explains the relationship between self-reported beliefs and behaviors of nurse practitioners toward gay and lesbian clients and reported nursing education related to cultural competence. He also identifies strategies to incorporate LGBT health into nursing curricula.

Empty Metal Jacket: The Biopolitical Economy Of War And Medicine, Sandra Lee Trappen

Dissertations, Theses, and Capstone Projects

Empty Metal Jacket: The Biopolitical Economy of War and Medicine undertakes study of how global conflict and violence shape the entire range of social production, from commodities and culture to social goods and social theory. The research presented in this work draws from cutting-edge theories in body and science studies, in addition to theories of affect and biopolitics to address how war became a problem solving paradigm in medicine. Combat casualties are shown to serve as a material nexus for medical knowledge production. Although the focus here is on medicine and medical innovation in particular, these developments are connected to …

To Foster A Culture Of Curiosity: A Hermeneutic Study Of The Experienced Nurse Educator And Student Intellectual Curiosity In The Online Learning Environment, Bedelia H. Russell

Electronic Theses and Dissertations

Skills of inquiry are essential outcomes from a baccalaureate nursing education. Students who demonstrate intellectual curiosity can develop effective skills of inquiry. Nurse educators must place emphasis on teaching and learning strategies which engage student intellectual curiosity. However, the concept of intellectual curiosity is not well-studied across multiple contexts of teaching and learning environments within nursing education. In addition, there is little known about the experienced nurse educator and the meaning of student intellectual curiosity across multiple teaching and learning environments. With the increased emphasis on online teaching and learning in nursing education as a solution for expanding student access …

Automobile, Construction And Entertainment Business Sector Influences On Sedentary Lifestyles, Diana C. Parra, Thiago H. De Sá, Carlos A. Monteiro, Nicholas Freudenberg

Publications and Research

Sedentary lifestyles contribute to premature death and health inequalities. Researchers have studied personal and community-level determinants of inactivity but few have analyzed corporate influences. To reframe the public health debate on inactivity and open new doors for public sector intervention, we conducted a scoping review of evidence from several disciplines to describe how the business and political practices of the automobile, construction, and entertainment sectors have encouraged sedentary lifestyles. In the last 50 years, these industries have found it profitable to produce motor vehicles, housing, and entertainment, which intentionally or unintentionally discourage physical activity. Ceding primary authority for policy decisions …

Understanding The Mechanism And Extent Of Vlse Recombination In Borrelia Burgdorferi Using Next-Generation Sequencing, Surabhi Tyagi

Dissertations and Theses (Open Access)

B. burgdorferi, the causative agent of Lyme disease, have an elaborate antigenic variation system that involves varying the sequence of vlsE. Previous studies have shown that vlsE antigenic variation occurs continuously inside mammalian hosts. Variation has not been shown previously to occur in in vitro or in ticks. We hypothesized that the induction of vlsE recombination requires contact with dense arrays of host tissue cells and/or ECM components. To test this hypothesis, two methods, quantitative PCR and high-throughput sequencing were used determine the extent and nature of vlsE recombination within mouse tissues and in vitro model systems. Using these …

Can Children’S Museums Deliver Effective Health Outreach?: Evaluation Results Of The Healthworks! Be A Food Groupie Program For Elementary Students, Kathleen Ragsdale, Ginger Cross

Journal of Health Disparities Research and Practice

Objectives: We evaluated the Be A Food Groupie (BAFG) program’s impact on health/nutrition knowledge among elementary students who received BAFG at HealthWorks! children’s museum.

Methods: In 2012-2013, we conducted a matched comparison evaluation using pre-/post-tests among 446 intervention and 524 comparison students (N = 970) in Grades 3-5 recruited from 11 Mississippi schools to determine whether BAFG improved health/nutrition knowledge across three domains: 1) comprehending food labels, 2) understanding serving sizes, 3) understanding food groups.

Results: After controlling for pre-test scores, ANCOVA results indicate that intervention students scored significantly higher on the post-test across all three domains and across all …

Three-Dimensional Printing And Nanotechnology For Enhanced Implantable Materials, Karthik Kumar Tappa

Doctoral Dissertations

Orthopedic and oro-maxillofacial implants have revolutionized treatment of bone diseases and fractures. Currently available metallic implants have been in clinical use for more than 40 years and have proved medically efficacious. However, several drawbacks remain, such as excessive stiffness, accumulation of metal ions in surrounding tissue, growth restriction, required removal/revision surgery, inability to carry drugs, and susceptibility to infection. The need for additional revision surgery increases financial costs and prolongs recovery time for patients. These metallic implants are bulk manufactured and often do not meet patient's requirements. A surgeon must machine (cut, weld, trim or drill holes) them in order …

Professionalization Of Teaching In America: Two Case Studies Using Educational Research Experiences To Explore The Perceptions Of Preservice Teachers/Researchers, James E. Gentry, Credence Baker, Holly Lamb

Administrative Issues Journal

In 2013-2015, two faculty-led educational research studies were conducted, aided by five undergraduate preservice teachers/researchers (PSTR). Faculty-researchers designed a qualitative phenomenological-inquiry based methodology to examine the PSTR perceptions regarding their respective research experiences with faculty. Triangulation of the data was sought from narrative text interview transcripts and response essays by PSTR prior to and after each respective study. Using content analysis techniques and coding, five themes emerged. PSTR reported an interest in the educational research topic and the need for positive relationships with faculty research mentors to begin and remain active with a research study. The results indicated PSTR valued …

N-Acetyl Cysteine May Support Dopamine Neurons In Parkinson's Disease: Preliminary Clinical And Cell Line Data., Daniel A. Monti, George Zabrecky, Daniel Kremens, Tsao-Wei Liang, Nancy A. Wintering, Jingli Cai, Xiatao Wei, Anthony J. Bazzan, Li Zhong, Brendan Bowen, Charles Intenzo, Lorraine Iacovitti, Andrew B. Newberg

Marcus Institute of Integrative Health Faculty Papers

BACKGOUND: The purpose of this study was to assess the biological and clinical effects of n-acetyl-cysteine (NAC) in Parkinson's disease (PD).

METHODS: The overarching goal of this pilot study was to generate additional data about potentially protective properties of NAC in PD, using an in vitro and in vivo approach. In preparation for the clinical study we performed a cell tissue culture study with human embryonic stem cell (hESC)-derived midbrain dopamine (mDA) neurons that were treated with rotenone as a model for PD. The primary outcome in the cell tissue cultures was the number of cells that survived the insult …

Diagnostic Practices Of Autism Spectrum Disorders In Brazil, Ana Claudia Vesper Manier Dias

Honors Theses

In Brazil, in spite of over half a million students with special needs in primary education, little research has been conducted about ASD diagnostic practices. This involves learning about the training that professionals are receiving, what diagnostic practices are being implemented on the field, and whether those are being contextualized according to the Brazilian culture. Students with special needs are directly affected by the results of diagnostic practices, since identifying the individuals is one of the first steps. Lack of understanding about that stage may lead to the problematic realities of underidentification or overidentification, exclusion of children who should receive …

Describing The End Of Life Knowledge, Beliefs, And Preferences Of Alaska Native And American Indian Peoples, Colleen Marie Kelley

Theses and Dissertations

This dissertation is comprised of three manuscripts that will be submitted for publication, describing the end of life knowledge, beliefs, and preferences of Alaska Native and American Indian Peoples. The first manuscript is a literature review that examines what is known about the understanding about the end of life knowledge, beliefs, and preferences of Alaska Native and American Indian Peoples. The second manuscript offers results of an exploratory descriptive study designed to describe the end of life knowledge of hospice care services, beliefs about dying, and preferences for end of life care of Alaska Native and American Indian Peoples. Finally, …

Impacts Of An Artist Residency Program Informed By Social Action Art Therapy, Jessica Bui Lo

LMU Theses and Dissertations

This research explores the impact of a three day Artist Residency Program at a K-8th public school on a Native American Reservation. The program is evaluated through a lens of Social Action Art Therapy and aims to uncover how this program impacted a community— including teachers, students, and parents, and facilitators. The researcher examined social action literature, social action art literature, social action art therapy literature, as well as social action with Native Americans literature. The researcher used a qualitative approach, specifically an Inquisitive Case study, in which data was collected through the researchers notes. These notes then informed the …

Reflect, React, Exchange: A Cultural Competency Co-Curriculum, Derrick Lewis

Capstone Collection

Cultural competency serves as key component to medical training and education and increasing interest in international health experiences denotes a recent need to respond to globalized health and populations. Reflect, React, Exchange (RRE) is a co-curriculum aims to provide a revised and integrated framework and foster awareness via experience, exchange, reflection, and dialogue at the Clinica de Familia La Romana in La Romana, Dominican Republic. RRE utilizes theories which are appropriate to the transformative learning aims and integral curricular activities of the CFLR Global Health Experience, an 8-week, global health internship experience for health science. It provides students with the …

Challenges Experienced By Korean Medical Students And Tutors During Problem-Based Learning: A Cultural Perspective, Hyunjung Ju, Ikseon Choi, Byoung Doo Rhee, Jong Tae-Lee

Interdisciplinary Journal of Problem-Based Learning

How people learn is influenced by the cultural contexts in which their learning occurs. This qualitative case study explored challenges Korean medical students and tutors experienced during their PBL sessions from a cultural perspective using Hofstede’s cultural dimensions. Twelve preclinical medical students and nine tutors from a large Korean medical school participated in interviews. The interview data were analyzed using the constant comparative method and classified according to Hofstede’s cultural dimensions. Twenty-two themes emerged within the following overarching categories: large power distance (6 themes), high uncertainty avoidance (6), individualism (3), collectivism (4), and masculinity/short-term orientation (3). This article discusses culturally …

Qualitative Exploration Of Spirituality In Heart Failure Patients, Abigail M. Reggio

The Eleanor Mann School of Nursing Undergraduate Honors Theses

Background.

Heart failure (HF) is a severe, progressive disease that afflicts large numbers of

individuals around the world. One significant factor affecting quality of life in persons

with HF is the extent to which spiritual needs are met. Developing an understanding of

spirituality in the HF patient is essential in order for the healthcare professional to

provide the highest quality care possible. This study focused on examining spirituality

in HF patients by means of conducting in-depth interviews with individuals diagnosed

with HF and completing a descriptive analysis of the responses. The goal of this study

was to gain insight into …

Exploring Dietary Intakes Of Micronutrients Involved In Bone Remodeling In Relation To Bone Mineral Density And Physical Activity Levels: An Examination Of Pre-Menarcheal Female Gymnasts And Non-Gymnasts., Sarah Kristine Skinner

Dissertations - ALL

Objective: The purpose of this study was to explore the relationships between dietary intake of vitamin K, vitamin A, vitamin D, fiber, and bone mineral density in a sample of pre-menarcheal female gymnasts and non-gymnasts in the Syracuse-area. The goal was to observe whether higher intakes of these vitamins were associated with higher bone mineral density as measured by dual-energy X-ray absorptiometry (DXA), and to potentially examine the impact of activity level on these relationships.

Methods: A cross-sectional design was used to examine a subset of data from a larger on-going longitudinal study on bone growth and physical activity in …

Ips Cell Transplantation For Traumatic Spinal Cord Injury., Miguel Goulao, Angelo C Lepore

Farber Institute for Neuroscience Faculty Papers

A large body of work has been published on transplantation of a wide range of neural stem and progenitor cell types derived from the developing and adult CNS, as well as from pluripotent embryonic stem cells, in models of traumatic spinal cord injury (SCI). However, many of these cell-based approaches present practical issues for clinical translation such as ethical cell derivation, generation of potentially large numbers of homogenously prepared cells, and immune rejection. With the advent of induced Pluripotent Stem (iPS) cell technology, many of these issues may potentially be overcome. To date, a number of studies have demonstrated integration, …

Descriptive Epidemiology Of Somatising Tendency: Findings From The Cupid Study., Sergio Vargas-Prada, David Coggon, Georgia Ntani, Georgia Ntani, Karen Walker-Bone, Keith T. Palmer, Vanda E. Felli, Raul Harari, Lope H. Barrero, Sarah A. Felknor, David Gimeno, Anna Cattrell, Matteo Bonzini, Eleni Solidaki, Eda Merisalu, Rima R. Habib, Farideh Sadeghian, Muhammad Masood Kadir, Sudath S. P. Warnakulasuriya, Ko Matsudaira, Busisiwe Nyantumbu, Malcolm R. Sim, Helen Harcombe, Ken Cox, Leila M. M. Sarquis, Maria H. Marziale, Florencia Harari, Rocio Freire, Natalia Harari, Magda V. Monroy, Leonardo A. Quintana, Marianela Rojas, E. Clare Harris, Consol Serra, J. Miguel Martinez, George Delclos, Fernando G. Benavides, Michele Carugno, Marco M. Ferrario, Angela C. Pesatori, Leda Chatzi, Panos Bitsios, Manolis Kogevinas, Kristel Oha, Tiina Freimann, Ali Sadeghian, Roshini J. Peiris-John, Nalini Sathiakumar, A. Rajitha Wickremasinghe, Noriko Yoshimura, Helen L. Kelsall, Victor C. W. Hoe, Donna M. Urquhart, Sarah Derrett, David Mcbride, Peter Herbison, Andrew Gray, Eduardo J. Salazar Vega

Community Health Sciences

Somatising tendency, defined as a predisposition to worry about common somatic symptoms, is importantly associated with various aspects of health and health-related behaviour, including musculoskeletal pain and associated disability. To explore its epidemiological characteristics, and how it can be specified most efficiently, we analysed data from an international longitudinal study. A baseline questionnaire, which included questions from the Brief Symptom Inventory about seven common symptoms, was completed by 12,072 participants aged 20-59 from 46 occupational groups in 18 countries (response rate 70%). The seven symptoms were all mutually associated (odds ratios for pairwise associations 3.4 to 9.3), and each contributed …

Enhancing Nurse-Physician Communication And Collaboration, Pamela D. Missi

Graduate Theses, Dissertations, and Capstones

In healthcare organizations today, creating a culture of safety is critically important. Communication failures among healthcare providers have been linked to 70% of annual sentinel events. Seventy-six percent of individuals experiencing a sentinel event die (Joint Commission, 2009). Lack of good communication behavior between nurses and physicians has been recognized as a cause of preventable harm to patients (Institute of Medicine, 2004; Zwarenstein & Reeves, 2006). In hospital settings, communication failures are linked to increases in length of stay, patient harm, resource utilization, more rapid turnover, and caregiver dissatisfaction (Dingley et al., 2008). Numerous national organizations and commissions have officially …

Embryo Adoption As An Ethical Option For Couples Faced With Infertility, Rachel M. Walters

Senior Honors Theses

Embryo adoption takes embryos that are still forming and introduces them into the uterus of a woman with whom they do not share any genetic material. If pregnancy does ensue and continues without complications, the child that will be born will not share any genes with the woman who carried him or her. In addition, if the child is born to parents who already have other children, the children in the adoptive family will share no biological link to the newborn. Essentially, the child will have two families—a biological family and an adoptive family. This thesis will explore and discuss …

Influence Of The 3d Microenvironment On Glioblastoma Migration And Drug Response, Ruth Marisol Herrera Perez

Open Access Dissertations

Glioblastoma (GBM) is a highly invasive brain cancer characterized by poor prognosis. Despite significant efforts by the basic and clinical research community our understanding of GBM progression and recurrence has been incremental. Improvements in therapeutic response have been dismal, and GBM continues to be the deadliest tumor of the central nervous system, with patient average survival rate of 12 months. Synergistic relationships that the tumor cells establish with the brain microenvironment have been proven fundamental for successful tumor progression and maintenance. Yet, many in vitro GBM studies are performed in formats that fail to recapitulate the most essential component of …

Predicting Externalizing Behaviors In Latino Adolescents Using Parenting And Educationalfactors, Sergio Benjamin Pereyra

Theses and Dissertations

Externalizing behaviors among adolescents continue to concern researchers and clinicians nationwide, especially among Latinos who are part of the largest and fastest growing minority population in the U. S. This dissertation begins by describing an eco-developmental model, which provides the theoretical framework used to conceptualize the systemic factors being studied and by reviewing the relevant literature regarding the influence of parental warmth, parental behavioral control, the adolescent-teacher relationship, and academic achievement on externalizing behavior among Latino adolescents. This study uses the National Longitudinal Study of Adolescent Health survey (Add Health) data to analyze direct and indirect effects of parenting and …

Chondroinduction From Naturally Derived Cartilage Matrix: A Comparison Between Devitalized And Decellularized Cartilage Encapsulated In Hydrogel Pastes., Emily C. Beck, Marilyn Barragan, Tony B. Libeer, Sarah L. Kieweg, Gabriel L. Converse, Richard A. Hopkins, Cory J. Berkland, Michael S. Detamore

Manuscripts, Articles, Book Chapters and Other Papers

Hydrogel precursors are liquid solutions that are prone to leaking after surgical placement. This problem was overcome by incorporating either decellularized cartilage (DCC) or devitalized cartilage (DVC) microparticles into traditional photocrosslinkable hydrogel precursors in an effort to achieve a paste-like hydrogel precursor. DCC and DVC were selected specifically for their potential to induce chondrogenesis of stem cells, given that materials that are chondroinductive on their own without growth factors are a revolutionary goal in orthopedic medicine. We hypothesized that DVC, lacking the additional chemical processing steps in DCC to remove cell content, would lead to a more chondroinductive hydrogel with …

Age And Employee Green Behaviors: A Meta-Analysis, Brenton M. Wiernik, Stephan Dilchert, Deniz S. Ones

Publications and Research

Recent economic and societal developments have led to an increasing emphasis on organizational environmental performance. At the same time, demographic trends are resulting in increasingly aging labor forces in many industrialized nations. Commonly held stereotypes suggest that older workers are less likely to be environmentally responsible than younger workers. To evaluate the degree to which such age differences are present, we meta-analyzed 132 independent correlations and 336 d-values based on 4676 professional workers from 22 samples in 11 countries. Contrary to popular stereotypes, age showed small positive relationships with pro-environmental behaviors, suggesting that older adults engaged in these workplace …