Digital Commons Network^™

Glycomics: Immunoglobulin Gn-Glycosylation Associated With Mammary Gland Hyperplasia In Women, Zixiu Meng, Cancan Li, Guoyong Ding, Weijie Cao, Xizhu Xu, Yuanyuan Heng, Yang Deng, Yuejin Li, Xiaoyu Zhang, Dong Li, Wei Wang, Youxin Wang, Weijia Xing, Haifeng Hou

Research outputs 2014 to 2021

Mammary gland hyperplasia (MGH) is very common, especially among young and middle-aged women. New diagnostics and biomarkers for MGH are needed for rational clinical management and precision medicine. We report, in this study, new findings using a glycomics approach, with a focus on immunoglobulin G (IgG) N-glycosylation. A cross-sectional study was conducted in a community-based population sample in Beijing, China. A total of 387 participants 40-65 years of age were enrolled in this study, including 194 women with MGH (cases) and 193 women who had no MGH (controls). IgG N-glycans were characterized in the serum by ultra-performance liquid chromatography. The …

Addressing The Batch Effect Issue For Lc/Ms Metabolomics Data In Data Preprocessing., Qin Liu, Douglas Walker, Karan Uppal, Zihe Liu, Chunyu Ma, Vilinh Tran, Shuzhao Li, Dean P Jones, Tianwei Yu

Faculty Research 2020

With the growth of metabolomics research, more and more studies are conducted on large numbers of samples. Due to technical limitations of the Liquid Chromatography-Mass Spectrometry (LC/MS) platform, samples often need to be processed in multiple batches. Across different batches, we often observe differences in data characteristics. In this work, we specifically focus on data generated in multiple batches on the same LC/MS machinery. Traditional preprocessing methods treat all samples as a single group. Such practice can result in errors in the alignment of peaks, which cannot be corrected by post hoc application of batch effect correction methods. In this …

Metabolism Of Jq1, An Inhibitor Of Bromodomain And Extra Terminal Bromodomain Proteins, In Human And Mouse Liver Microsomes†, Feng Li, Kevin R Mackenzie, Prashi Jain, Conrad Santini, Damian W Young, Martin M Matzuk

Faculty, Staff and Students Publications

JQ1 is a small-molecule inhibitor of the bromodomain and extra terminal (BET) protein family that potently inhibits the bromodomain testis-specific protein (BRDT), which is essential for spermatogenesis. JQ1 treatment produces a reversible contraceptive effect by targeting the activity of BRDT in mouse male germ cells, validating BRDT as a male contraceptive target. Although JQ1 possesses favourable physical properties, it exhibits a short half-life. Because the details of xenobiotic metabolism play important roles in the optimization of drug candidates and in determining the role of metabolism in drug efficacy, we investigated the metabolism of JQ1 in human and mouse liver microsomes. …

Bortezomib With Standard Chemotherapy For Children With Acute Myeloid Leukemia Does Not Improve Treatment Outcomes: A Report From The Children's Oncology Group., Richard Aplenc, Soheil Meshinchi, Lillian Sung, Todd Alonzo, John Choi, Brian Fisher, Robert Gerbing, Betsy Hirsch, Terzah Horton, Samir Kahwash, John Levine, Michael Loken, Lisa Brodersen, Jessica Pollard, Susana Raimondi, Edward Anders Kolb, A S. Gamis

Manuscripts, Articles, Book Chapters and Other Papers

New therapeutic strategies are needed for pediatric acute myeloid leukemia (AML) to reduce disease recurrence and treatment-related morbidity. The Children's Oncology Group Phase III AAML1031 trial tested whether the addition of bortezomib to standard chemotherapy improves survival in pediatric patients with newly diagnosed AML. AAML1031 randomized patients younger than 30 years of age with de novo AML to standard treatment with or without bortezomib. All patients received the identical chemotherapy backbone with either four intensive chemotherapy courses or three courses followed by allogeneic hematopoietic stem cell transplantation for high-risk patients. For those randomized to the intervention arm, bortezomib 1.3 mg/m2 …

Perspectives On The Intracellular Bacterium Chlamydia Pneumoniae In Late-Onset Dementia, Brian J. Balin Phd

PCOM Scholarly Works

Purpose of Review

Chronic diseases remain a daunting challenge for clinicians and researchers alike. While difficult to completely understand, most chronic diseases, including late-onset dementias, are thought to arise as an interplay between host genetic factors and environmental insults. One of the most diverse and ubiquitous environmental insults centers on infectious agents. Associations of infectious agents with late-onset dementia have taken on heightened importance, including our investigations of infection by the intracellular respiratory bacterium, Chlamydia pneumoniae (Cpn), in late-onset dementia of the Alzheimer’s type.

Recent Findings

Over the last two decades, the relationship of this infection to pathogenesis …

Synthesis, Characterisation And Biological Evaluation Of Tyramine Derived Schiff Base Ligand And Its Transition Metal(Ii) Complexes, Abdul Khader Jailani, N.S.K. Gowthaman, Mookkandi Palsamy Kesavan

Karbala International Journal of Modern Science

In this study, a new tyramine derived Schiff base ligand (L) (L=1,3-phenylene-bis-4-aminoantipyrinyl-4-aminoethylphenol) and its derived transition metal(II) complexes [Cu(L)Cl₂](1), [Ni(L)Cl₂](2), [Co(L)Cl₂] (3) and [Zn(L)Cl₂] (4) have been synthesized and well characterized by the way of different spectroscopic and analytical techniques. Analytical and spectroscopic studies result suggests that metal(II) complexes more probably have octahedral geometry. DNA binding tendency of L and metal(II) complexes 1-4 have been assessed by probing their ability to bind with Calf Thymus DNA (CT-DNA) via electronic absorption and cyclic voltammetry titration methods. The results clearly reveal that the metal(II) …

Proteolysis-Targeting Chimera (Protac) For Targeted Protein Degradation And Cancer Therapy, Xin Li, Yongcheng Song

Faculty, Staff and Students Publications

Proteolysis-targeting chimera (PROTAC) has been developed to be a useful technology for targeted protein degradation. A bifunctional PROTAC molecule consists of a ligand (mostly small-molecule inhibitor) of the protein of interest (POI) and a covalently linked ligand of an E3 ubiquitin ligase (E3). Upon binding to the POI, the PROTAC can recruit E3 for POI ubiquitination, which is subjected to proteasome-mediated degradation. PROTAC complements nucleic acid-based gene knockdown/out technologies for targeted protein reduction and could mimic pharmacological protein inhibition. To date, PROTACs targeting ~ 50 proteins, many of which are clinically validated drug targets, have been successfully developed with several …

S-Seco-Porphyrazine As A New Member Of The Seco-Porphyrazine Family – Synthesis, Characterization And Photocytotoxicity Against Cancer Cells, Dariusz T. Mlynarczyk, Jaroslaw Piskorz, Lukasz Popenda, Magdalena Stolarska, Wojciech Szczolko, Krystyna Konopka, Stefan Jurga, Lukasz Sobotta, Jadwiga Mielcarek, Nejat Düzgüneş, Tomasz Goslinski

All Dugoni School of Dentistry Faculty Articles

An important subgroup within the porphyrazine (Pz) family constitutes seco-porphyrazines, in the chemical structure of which one pyrrole unit is opened in the oxidative process. So far, there are only limited data on N-seco- and C-seco-Pzs. Here, the synthesis of a novel member of the Pzs seco-family, represented by an S-seco-tribenzoporphyrazine analogue, 22,23-bis(4-(3,5-dibutoxycarbonylphenoxy)butylsulfanyl)tribenzo[b,g,l]-22,23-dioxo-22,23-seco-porphyrazinato magnesium(II), is reported, with moderate 34% yield. The new derivative was characterized using NMR spectroscopy, UV–Vis spectroscopy, and mass spectrometry. In the photochemical study performed following the indirect chemical method with 1,3-diphenylisobenzofuran, S-seco-Pz revealed a high singlet oxygen quantum yield of 0.27 in DMF. Potential photocytotoxicity of …

Metabolic Dysregulation In The Atp7b-/- Wilson's Disease Mouse Model, Clavia Ruth Wooton-Kee, Matthew Robertson, Ying Zhou, Bingning Dong, Zhen Sun, Kang Ho Kim, Hailan Liu, Yong Xu, Nagireddy Putluri, Pradip Saha, Cristian Coarfa, David D Moore, Alli M Nuotio-Antar

Faculty, Staff and Students Publications

Inactivating mutations in the copper transporter Atp7b result in Wilson’s disease. The Atp7b−/− mouse develops hallmarks of Wilson’s disease. The activity of several nuclear receptors decreased in Atp7b−/− mice, and nuclear receptors are critical for maintaining metabolic homeostasis. Therefore, we anticipated that Atp7b−/− mice would exhibit altered progression of diet-induced obesity, fatty liver, and insulin resistance. Following 10 wk on a chow or Western-type diet (40% kcal fat), parameters of glucose and lipid homeostasis were measured. Hepatic metabolites were measured by liquid chromatography–mass spectrometry and correlated with transcriptomic data. Atp7b−/− mice fed a chow diet presented …

Cancer Care Annual Report 2019-2020, Children's Mercy Hospital

Cancer Center Annual Reports

Annual report of cancer care and oncology services at the Children's Mercy Hospital, Kansas City MO, a pediatric medical center. This year’s report focuses on tumors that arise in the kidney and illustrates three important areas of focus that a childhood cancer center must possess in this new age of medicine.

Omega-3 Fatty Acids, Kristina Krupa, Kristina Fritz, Mayur S. Parmar

HPD Articles

No abstract provided.

Oil/Water Nanoemulsion Biodistribution In Mice Upon Intravenous Administration, Jiayi Chen

Theses and Dissertations

The present study aimed to explore the biodistribution of O/W nanoemulsions (NE) upon intravenous administration. Three NEs were prepared with distinctive droplet sizes: SE (29 ± 1 nm), ME (214 ± 2 nm) and LE (883 ± 16 nm) without overlapping of the size distribution. Kolliphor® HS15 was used as the only surfactant for these three NEs, so that their droplets had similar surface structure. The NEs droplet size was stable under room temperature for minimum 3 days in phosphate buffer saline (PBS) and in mice plasma in vitro for 4-hour at 37°C. A lipophilic fluorescent dye, 1, 1’-dioctadecyl-3, 3, …

The Effects Of Postpartum Depression, Acculturative Stress, And Social Support On Mother-Infant Bonding Among U.S. Immigrant Women Of Arabic Descent, Darin Mansor Mathkor

Wayne State University Dissertations

Purpose and Background/Significance: The transition from “woman” to “mother” is a significant life event and can be extremely stressful when merged with the transition from ‘local’ to ‘immigrant’. The development of mother-infant bonding is considered a critical process in the postpartum period. Immigrant women have higher rates of PPD compared with women in their native countries. Among the general population, PPD is strongly associated with lower quality mother-infant bonding. Social support can play a central role in lowering the levels of PPD, and may contribute to higher quality of mother-infant bonding. Moreover, limited data suggests that acculturative stress is found …

Drug Discovery Targeting The Disorder-To-Order Transition Regions Through The Conformational Diversity Mimicking And Statistical Analysis, Insung Na, Sungwoo Choi, Seung Han Son, Vladimir N. Uversky, Chul Geun Kim

Molecular Medicine Faculty Publications

Intrinsically disordered proteins exist as highly dynamic conformational ensembles of diverse forms. However, the majority of virtual screening only focuses on proteins with defined structures. This means that computer-aided drug discovery is restricted. As a breakthrough, understanding the structural characteristics of intrinsically disordered proteins and its application can open the gate for unrestricted drug discovery. First, we segmented the target disorder-to-order transition region into a series of overlapping 20-amino-acid-long peptides. Folding prediction generated diverse conformations of these peptides. Next, we applied molecular docking, new evaluation score function, and statistical analysis. This approach successfully distinguished known compounds and their corresponding binding …

Profiling Transcription And Retrotransposition Of Mouse L1 Subfamilies, Lingqi Kong

Electronic Theses and Dissertations

Transposable elements, also called jumping genes, comprise almost 45% of the human genome. In contrast, only 1% of the human genome is protein-coding sequences. The function and advantages of maintaining such massive copies of transposable elements in the human genome are still unclear. Long interspersed element 1 (L1), the most substantial group and the only active autonomous transposable element in the human genome, has revealed its unique roles in many diseases. The insertional mutagenesis induced by L1 retrotransposition events could threaten human genomic stability and generate unexpected mutations. L1 overexpression has been documented in both somatic and germline cells and, …

Comparability Of Raman Spectroscopic Configurations: A Largescale Cross-Laboratory Study, Shuxia Guo, Hugh Byrne, Shiyamala Duraipandian, Fiona Lyng, Claudia Beleites, Ute Neugebauer

Articles

The variable configuration of Raman spectroscopic platforms is one ofthe major obstacles in establishing Raman spectroscopy as a valuable physicochemicalmethod within real-world scenarios such as clinical diagnostics. For such real worldapplications like diagnostic classification, the models should ideally be usable to predictdata from different setups. Whether it is done by training a rugged model with data frommany setups or by a primary-replica strategy where models are developed on a‘primary’setup and the test data are generated on‘replicate’setups, this is only possible if the Raman spectra from different setups are consistent, reproducible, and comparable.However, Raman spectra can be highly sensitive to the …

Spatially Fractionated Radiation Therapy: History, Present And The Future, Weisi Yan, Mohammad K. Khan, Xiaodong Wu, Charles B. Simone Ii, Jiajin Fan, Eric Gressen, Xin Zhang, Charles L. Limoli, Houda Bahig, Slavisa Tubin, Waleed F. Mourad

Department of Radiation Oncology Faculty Papers

No abstract provided.

Bmp And Tgfß Use And Release In Bone Regeneration, Zeynep Bal, Junichi Kushioka, Joe Kodama, Takashi Kaito, Hideki Yoshikawa, Petek Korkusuz, Feza Korkusuz

Turkish Journal of Medical Sciences

A fracture that does not unite in nine months is defined as nonunion. Nonunion is common in fragmented fractures and large bone defects where vascularization is impaired. The distal third of the tibia, the scaphoid bone or the talus fractures are furthermore prone to nonunion. Open fractures and spinal fusion cases also need special monitoring for healing. Bone tissue regeneration can be attained by autografts, allografts, xenografts and synthetic materials, however their limited availability and the increased surgical time as well as the donor site morbidity of autograft use, and lower probability of success, increased costs and disease transmission and …

Hormonal Regulation Of Glycine Decarboxylase And Its Metabolic Outcomes, Ruta Milind Jog

Wayne State University Dissertations

The amino acid glycine is involved in generation of multiple critical metabolites including glutathione, heme, and creatinine. Interestingly, in both humans and rodents, circulating glycine levels are significantly reduced in obesity, glucose intolerance, type II diabetes and non-alcoholic fatty liver disease. The glycine cleavage system is the predominant glycine degradation pathway in humans. The rate-limiting enzyme of glycine cleavage system is glycine decarboxylase (GLDC), and loss-of-function mutations of GLDC cause hyperglycinemia. Here, we show that GLDC gene expression is upregulated in livers of mouse models of diabetes and diet-induced obesity as well as in the fasted state in normal animals. …

Non-Uniformity Of Projection Distributions Attenuates Resolution In Cryo-Em, Philip R Baldwin, Dmitry Lyumkis

Faculty, Staff and Students Publications

Virtually all single-particle cryo-EM experiments currently suffer from specimen adherence to the air-water interface, leading to a non-uniform distribution in the set of projection views. Whereas it is well accepted that uniform projection distributions can lead to high-resolution reconstructions, non-uniform (anisotropic) distributions can negatively affect map quality, elongate structural features, and in some cases, prohibit interpretation altogether. Although some consequences of non-uniform sampling have been described qualitatively, we know little about how sampling quantitatively affects resolution in cryo-EM. Here, we show how inhomogeneity in any projection distribution scheme attenuates the global Fourier Shell Correlation (FSC) in relation to the number …

Copper/N-Acetylcysteine Coated Iron Oxide Nanoparticles Synthesis, Characterization, And Antimicrobial Activities, Danya Belnour

Electronic Theses and Dissertations, 2020-2023

In recent years, there is a growing interest in developing metal based antimicrobial nanomaterials suitable for agricultural and biomedical applications. For centuries, Copper (Cu) biocide has been used for protecting a wide variety of crops from devastating bacterial and fungal diseases. However, prolonged and aggressive use of Cu led to the development of resistance and accumulation in soil. The latter has been linked to aquatic toxicity due to soil Cu leaching. Furthermore, copper build up in soil causes phytotoxicity and reduces uptake of micronutrient Zn through the root system. In biomedical field, Cu has been historically used as an antimicrobial …

Attitudes Toward The Hpv Vaccine Among University Students: Identifying Barriers To Vaccine Uptake, Kristina L. Harbaugh

College of Graduate Studies: Theses & Dissertations

Background: In the United States, the Centers for Disease Control and Prevention (CDC) estimates that 32,000 cancers occur annually that can be directly attributable to the acquisition of the Human Papilloma Virus (HPV). Gender specificity data posits that 21,000 of the annual cancer cases are female and the remaining 11,000 cases occur among males. However, because the implementation and trajectory of HPV vaccination and prevention programs have been historically aimed toward women, vaccination rates for men remain significantly lower. The purpose of this study was to assess gender differences in knowledge attitude, subjective norms and perceived behavioral control about HPV …

The Effect Of Laser Power On Mechanical And Physical Properties Of Ti-6al-4v Eli Fabricated By Selective Laser Melting, Pajaree Termrungruanglert

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to examine the differences in mechanical and physical properties of Ti-6Al-4V extra low interstitial (ELI) fabricated by selective laser melting (SLM) between different laser power. The Ti-6Al-4V ELI alloy samples were printed in dumbbell shape by SLM machine (Trumpf/TruPrint 1000, Germany) with 3 laser powers (75, 100 and 125 W), 8 samples for each group. And the other parameters (spot size 30 µm, scanning speed 600 mm/s, layer thickness 30 µm) were kept constantly. All samples were performed under tensile test with universal testing machine. Moreover, The microhardness test was performed. All data were statistically analyzed with …

Pharmacokinetic And Metabolomic Studies Of Standardized Extract Of Centella Asiatica (Eca 233) Capsule In Thai Healthy Volunteers, Phanit Songvut

Chulalongkorn University Theses and Dissertations (Chula ETD)

A well-characterized standardized extract of Centella asiatica (ECa 233) being developed as a phytopharmaceutical product for human use was explored in this clinical study. The first objective of this research was to understand changes in disposition kinetics after oral administration of both the original-formula capsule and a newly-modified enhanced-dissolution capsule of ECa 233. Secondly, this study aimed to investigate alteration of human metabolomes following administration of the modified capsule of ECa 233, using an NMR-based metabolomics approach. For pharmacokinetics, the study involved a phase I clinical trial in twelve healthy Thai volunteers, who each received 250 and 500 mg of …

Network-Based Analytics For Discovering Gene Modules And Biomarkers In Complex Diseases, Zongliang Yue

All ETDs from UAB

With rapidly increasing novel discoveries of associations between genomic data and complex disease phenotypes, the translation of those associations into knowledge and the identification of critical molecular mechanisms are essential to guild clinical decisions such as the diagnosis of symptomatic individuals, the prediction of disease risk, reproductive genetic counseling, and determining pharmacogenetic profiles for treatment. To decipher the genome of complex diseases, gene-centric analyses focus on detecting molecular mechanisms lead by driver genetics variants, abnormally expressed genes and high-abundance proteins through gene module construction. Additionally, the gene module construction approaches implement systems biology analysis to reveal the causative co-expressed gene …

Novel Hiv Adaptation Strategy Against Host Cd8 T Cell Immunity, Kai Qin

All ETDs from UAB

Since the 1980s, HIV infection has caused millions of death and tremendous economic losses worldwide. Despite great scientific advances, the only effective therapy to date, antiretroviral therapy (ART), merely delays disease progression yet cannot eradicate viral infection. Therefore, an effective HIV vaccine that can prevent the acquisition of infection would be a desirable alternative. The critical role of CD8 T-cell immunity in HIV infection has been well documented and therefore not surprisingly a great deal of effort has been focused on developing an effective CD8 T-cell based HIV vaccine. However, by far, such CD8 T-cell based HIV vaccine trials have …

Application Of A Brain-Inspired Spiking Neural Network Architecture To Odor Data Classification, Anup Vanarse, Josafath Israel Espinosa-Ramos, Adam Osseiran, Alexander Rassau, Nikola Kasabov

Research outputs 2014 to 2021

Existing methods in neuromorphic olfaction mainly focus on implementing the data transformation based on the neurobiological architecture of the olfactory pathway. While the transformation is pivotal for the sparse spike-based representation of odor data, classification techniques based on the bio-computations of the higher brain areas, which process the spiking data for identification of odor, remain largely unexplored. This paper argues that brain-inspired spiking neural networks constitute a promising approach for the next generation of machine intelligence for odor data processing. Inspired by principles of brain information processing, here we propose the first spiking neural network method and associated deep machine …

Age At Diagnosis And Lung Cancer Presentation, Marida Gingras

Walden Dissertations and Doctoral Studies

Lung cancer is the leading cause of cancer-related mortality in the United States because of its lack of symptoms until late stages. It is noted that a 5-year relative survival rate can be improved by earlier cancer detection. The currently recommended age of screening by the U.S. Preventive Services Task Force may not be optimal, and the recommendations are only for smokers. The purpose of this cross-sectional study was to examine the association between the stages of presentation of lung cancer and age at diagnosis using quantitative research. Using the Surveillance, Epidemiology, and End Results (SEER-18) database, 63,107 records were …

Effects Of Dietary Guar Meal With Or Without Beta-Mannanase On Performance And Eggquality Traits In Laying Hens, Süleyman Çalişlar

Turkish Journal of Veterinary & Animal Sciences

In this study, the effects of beta-mannanase supplementation to laying hens? diets with different guar meal (GM) ratios on performance and egg quality traits were investigated. The experimental period lasted 126 days. Ninety-six Lohman Brown hens at the age of 56 weeks were kept in individual cages. They were divided into 8 treatment groups as follows: 0% guar meal (GM0) (control); 0% GM + 0.05% beta-mannanase (GM0E); 8% GM (GM8); 8% GM + 0.05% beta-mannanase (GM8E); 16% GM (GM16); 16% GM + 0.05% beta-mannanase (GM16E); 24% GM (GM24); and 24% GM + 0.05% beta-mannanase (GM24E). A photoperiod of 16.5 h …

Comparative Morphological Analysis Of Diploid And Triploid Oysters, Crassostrea Gigas,Farmed In The Black Sea, Oksana Yurievna Vialova

Turkish Journal of Veterinary & Animal Sciences

Triploid organisms, in particular oysters, are widely used in marine aquaculture. However, the advantage of triploid oysters compared to diploids has not been proven in all cases. Comparative morphological analysis of Pacific oysters Crassostrea gigas of different ploidy levels allows evaluating the benefits of raising triploids in the Black Sea. First, the morphological characteristics of diploid and triploid Pacific oysters C. gigas farmed in the Black Sea were studied. Allometric ratios of weight (W, g) and shell height (H, mm) of oysters were obtained, which are described by the equations W$_{2n}$ = 4 × 10$^{-4}$ × H$_{2n}$ $^{2.56}$, R2 = …