Digital Commons Network^™

Phage Infection, Transfection And Transformation Of Mycobacterium Avium Complex And Mycobacterium Paratuberculosis, Ellen M. Foley-Thomas, Diane L. Whipple, Luiz M. Bermudez, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium complex strains and Mycobacterium paratuberculosis are closely related intracellular pathogens affecting humans and animals. M. avium complex infections are a leading cause of morbidity and mortality in AIDS patients, and M. paratuberculosis is the agent of Johne's disease in ruminants. Genetic manipulation of these micro-organisms would facilitate the understanding of their pathogenesis, the construction of attenuated vaccine strains and the development of new drugs and treatment methods. This paper describes the replication of mycobacterial shuttle phasmids and plasmids, and the expression of the firefly luciferase reporter gene in M. avium complex and M. paratuberculosis. The mycobacteriophage TM4 …

Mmpi And Rorschach Findings Of Individuals Approved For Gender Reassignment Surgery, Gregory Ralph Caron

Psychology Theses & Dissertations

Both clinical experience and empirical data from psychological tests present a picture of extreme clinical variation among those individuals who request gender reassignment surgery. Results of past empirical studies utilizing the MMPI and the Rorschach Test have tended to be equivocal regarding the level and nature of psychopathology associated with samples of gender dysphorics. These past studies are considered limited particularly in terms of methodological problems related to statistical power. This present study examined the nature and degree of psychopathology in a sample of candidates approved for gender reassignment surgery as reflected on their MMPI-2 clinical scale values and scores …

What's Happening: September 6, 1995, Maine Medical Center

What's Happening

No abstract provided.

Temporal Changes In The Levels Of Gamma-Aminobutyric Acid In The Brain Of The Cockroach, Leucophaea Maderae, Jonathan Newton

Masters Theses & Specialist Projects

Circadian rhythms have been shown to exist in all studied species and play an important role in their survival. The cockroach is an excellent model organism in which to study circadian systems because of the wealth of background information regarding both its anatomy and circadian system. Clock activity measured electrophysiologically in the cockroach system is 180 degrees out of phase with locomotor activity, with the maximum neural output occurring in the morning, twelve hours prior to the onset of locomotor activity. This inverse relationship of locomotor and neural activity suggests that the clock output may be inhibitory. In vertebrates, the …

Expression Of Genes Encoding Antioxidant Enzymes In Preimplantation Mouse And Cow Embryos And Primary Bovine Oviduct Cultures Employed For Embryo Coculture., M B Harvey, M Y Arcellana-Panlilio, X Zhang, G A Schultz, A J Watson

Obstetrics & Gynaecology Publications

Preimplantation embryos from a variety of mammalian species contrast markedly in their response to culture in vitro. Murine preimplantation embryos display a wider tolerance than other mammalian species to culture environments, and this has contributed to the development of several effective defined culture media. Embryo coculture on somatic cells remains the most effective method of supporting reasonable rates of bovine preimplantation development in vitro. The patterns of gene expression for several antioxidant enzymes during preimplantation murine and bovine development were examined by use of the reverse transcription-polymerase chain reaction technique to determine whether the differential developmental capacity of mammalian preimplantation …

Involvement Of P59FynT In Interleukin-5 Receptor Signaling, Mark W. Appleby, James D. Kerner, Sylvia Chien, Charles R. Maliszewski, Subbarao Bondada, Roger M. Perlmutter

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Previous studies implicate the nonreceptor protein tyrosine kinase (PTK) p59fyn in the propagation of signals from the B cell antigen receptor. To elucidate the functions of this kinase, we examined B cell responsiveness in mice engineered to lack the hematopoietic isoform of p59fyn. Remarkably, antigen receptor signaling was only modestly defective in fyn^Tnull B cells. In contrast, signaling from the interleukin (IL)-5 receptor which ordinarily provides a comitogenic stimulus with antiimmunoglobulin, was completely blocked. Our results document the importance of p59fyn^T in IL-5 responses in B cells, and they support a general model …

L'Absorption Percutanée Des Contaminants Du Milieu De Travail, Claude Viau, Diane Talbot, Adolf Vyskocil

Rapports de recherche scientifique

No abstract provided.

Menno-Knight, September 1995, Mennonite College Of Nursing

Menno-Knight (1960s-1990s?)

Student newsletter of the Mennonite School/College of Nursing.

EnterohemorrhagicEscherichia Coli O157:H7 Requires Intimin To Colonize The Gnotobiotic Pig Intestine And To Adhere To Hep-2 Cells, Marian L. Mckee, Angela R. Melton-Celsa, Rodney A. Moxley, David H. Francis, Alison D. O'Brien

School of Veterinary and Biomedical Sciences: Faculty Publications

In a previous study, enterohemorrhagic Escherichia coli (EHEC) O157:H7 with a deletion and insertion in the eaeA gene encoding intimin was used to establish that intimin is required for the organism to attach to and efface microvilli in the piglet intestine (M. S. Donnenberg, S. Tzipori, M. L. McKee, A. D. O’Brien, J. Alroy, and J. B. Kaper, J. Clin. Invest. 92:1418–1424, 1993). However, in the same investigation, a role for intimin in EHEC adherence to HEp-2 cells could not be definitively demonstrated. To analyze the basis for this discrepancy, we constructed an in-frame deletion of eaeA and compared the …

Identification Of Gene Products Encoded By The Latency-Related Gene Of Bovine Herpesvirus 1, Ashfaque Hossain, L. M. Schang, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus 1 (BHV-1) establishes a latent infection in sensory ganglionic neurons of infected animals. Expression of latency-related (LR) gene products is controlled by a 980-bp fragment (LR promoter). DNA sequence analysis revealed that two major open reading frames (ORFs) are in the LR gene. Antibodies directed against both ORFs were generated in rabbits by using synthetic peptides. Antibody P2, which is directed to sequences near the amino terminus of ORF 2, recognized a 41-kDa protein in lytically infected cells, suggesting that ORF 2 encodes a protein. When the LR gene was inserted into a mammalian expression vector and subsequently …

Freshwater-Snail Intermediate Hosts Of Animal Parasitic Diseases In Thailand(หอยน้ำจืดที่เป็นพาหะกลางของโรคพยาธิของสัตว์ในประเทศไทย, Chantima Lohachit, Suwanee Nitiuthai

The Thai Journal of Veterinary Medicine

Thailand has one of the richest freshwater small fauna in the world; over 170 species are currently recognized. However, species that transmit animal diseases, or are suspected to, are relatively few. At present, only 23 freshwater snail species are implicated or suspected in animal disease transmission. These species are restricted to two orders and seven families. The parasites they transmit are blood flukes, liver flukes, lung flukes, intestinal flukes and some lung nematodes.(ประเทศไทยเป็นประเทศหนึ่งที่มีหอยน้ำจืดมากที่สุดในโลก ในปัจจุบันมีหอยน้ำจืดที่ได้ถูกค้นพบแล้วมากกว่า 170 ชนิด อย่างไรก็ตามชนิดของหอยที่นำโรคมาสู่สัตว์หรือสงสัยว่าอาจนำโรคมาสู่สัตว์นั้นค่อนข้างน้อย ในปัจจุบันมีหอยน้ำจืดเพียง 23 ชนิด ที่เกี่ยวข้องหรือ อาจเกี่ยวข้องในการนำโรคมาสู่สัตว์ พวกหอยเหล่านี้จัดอยู่ใน 2 อันดับ และ 7 วงศ์ พยาธิที่ใช้หอยน้ำจืดเป็นพาหะกลางคือ พยาธิใบไม้เลือด พยาธิใบไม้ตับ พยาธิใบไม้ลำไส้ และพยาธิตัวกลมในปอด)

Determination Of Cholinesterase Activity In Tiger Shrimp And Giant Perch(การวิเคราะห์หาสมรรถนะของเอนไซม์ โฆลีนเอสเทอเรสในกุ้งกุลาดําและปลากะพงขาว), Supatra Srichairat

The Thai Journal of Veterinary Medicine

The conventional colorimetric method of cholinesterase activity determination in blood and tissues of mammals was applied to investigate the enzyme activity in muscle and nervous cell of aquatic animals which are economically important, such as, tiger shrimp (Penaeus monodon fabricus) and giant perch (Lates calcarifer). Decreased enzyme activity in aquatic animals may indicate the contamination of insecticide in water resources. This study demonstrated that the method was suitable for detecting toxicity of organophosphate insecticide due to its convenience, simplicity and no sophisticated or expensive instruments needed. Moreover, the samples can be kept frozen for 2 weeks before analysing with less …

What's Happening: August 23, 1995, Maine Medical Center

What's Happening

No abstract provided.

Managing Household Hazardous Waste Or Making A Choice To Do Something Without Going Broke, Maine State Planning Office

Maine Collection

Managing Household Hazardous Waste Or Making a Choice to Do Something Without Going Broke

State of Maine, State Planning Office, Augusta, Maine

Androscoggin Valley Council of Governments, Auburn, Maine

August 1995.

Contents: Acknowledgements / Abbreviations / Introduction / I. History of Household Hazardous Waste in Maine / II. How-To Guide for Towns / Appendixes / Bibliography

Antibody Response To Trypanosoma Cruzi During The Course Of Infection In Mice Held At Elevated Environmental Temperature, Lianyong Gao

Masters Theses & Specialist Projects

It has been demonstrated that changes in environmental temperature can influence the resistance of mice to Trypanosoma cruzi (the causative agent of Chagas' disease in humans) infection. This increased resistance is not only reflected by decreased parasitemias and increased longevity but also by enhanced parasite-specific and nonspecific immune responsiveness in infected mice. The antibody response to heterologous antigen has been demonstrated to be greatly enhanced in infected mice held at elevated temperature. However, the parasite-specific antibody response was reported to be lower in infected mice maintained at 36°C than at room temperature (RT) during early stage of infection, although it …

The Effect Of Dietary Supplemented Vitamin E And Selenium On Experimental Chagas' Disease, Brenda Bennett

Masters Theses & Specialist Projects

Dietary supplementation with increased levels of vitamin E can enhance the immune system of C3HeB/FeJ mice to enable many of them to survive an otherwise lethal dose of Trypanosoma cruzi, the causative agent of Chagas' disease. To study the role of diet on the course of experimental Chagas' disease, C3HeB/FeJ mice were fed pelleted, synthetic food containing no vitamin E and selenium, 800 IU/kg vitamin E, 2 ppm selenium, or food containing 800 IU/kg vitamin E plus 2 ppm selenium. Mice on synthetic food were compared to control mice fed a commonly used Purina rodent chow # 5001. After diet …

Animal Activist Urges Vets To Be Activists Too, Henry Spira

Commentaries and Editorials

No abstract provided.

Site Directed Mutagenesis Of A Putative Protease Cleavage Site Within The 105 Kilodation Protein Of Southern Bean Mosaic Virus, Jingyu Shi

Masters Theses & Specialist Projects

There are four open reading frames (ORF) in three positive-sense translational phases of Southern Bean Mosaic Virus (SBMV). ORF2 codes for a 105 KDa polyprotein which is proposed to be autocatalytically cleaved into smaller functional proteins. Amino acid sequence homology between the SBMV and poliovirus, foot and mouth disease virus, and cowpea mosaic virus reveal that SBMV has a similar genomic organization to picornaviruses with a conserved order: Vpg (Viral protein, genome-linked )-protease-replicase. Two Gln-Ser (QS) amino acid pairs within the 105 KDa polyprotein, C75= 930-936, C60= 1305-1310, are proposed to be the cleavage sites based on 1) similar structural …

Alveolar Bone And Cementum Regeneration Following Periodontal Reconstructive Therapy : A Review Of Histologic Observations In Man And Experimental Animals, Karen E. Rodden

Loma Linda University Electronic Theses, Dissertations & Projects

Periodontal regeneration includes the formation of both alveolar bone and cementum. Studies reporting histologic evidence of healing following periodontal reconstructive therapy were reviewed to determine whether regeneration of these structures improves with increasing healing interval, and whether they regenerate independently or whether regeneration of one structure is dependent upon regeneration of the other. Additionally, the review addresses whether specific reconstructive modalities predictably yield increased amounts of regeneration. Differences in regenerative potential between humans, nonhuman primates, and canines were also examined. Results of over a decade of studies of periodontal reconstructive therapy were tabulated by family (human, nonhuman primate, and canine) …

Evaluation Of Nitric Oxide In Respiratory Distress Syndrome, Kimberly S. Myruski

Loma Linda University Electronic Theses, Dissertations & Projects

Little is known of the effects of low concentrations of inhaled nitric oxide (NO) on pulmonary mechanics and lung volumes in premature newborn infants with lung disease. Cardiopulmonary function tests including blood gas analyses were obtained from eleven premature lamb models with respiratory distress syndrome to determine if compliance, airways resistance, tidal volume, functional residual capacity, and gas exchange were affected. The effect of inhaled NO on the direction of shunting through the ductus arteriosus was determined using echocardiography.

Twin lambs were cannulated with arterial and venous catheters and intubated with an endotracheal tube before delivery on gestational day 126-130 …

Asbestos Induces Nuclear Factor Kappa B (Nf-Kappa B) Dna-Binding Activity And Nf-Kappa B-Dependent Gene Expression In Tracheal Epithelial Cells., Yvonne M. Janssen, Aaron Barchowsky, Melinda Treadwell, Kevin E. Driscoll, B T. Mossman

Dartmouth Scholarship

Nuclear factor kappa B (NF-kappa B) is a transcription factor regulating expression of genes intrinsic to inflammation and cell proliferation--features of asbestos-associated diseases. In studies here, crocidolite asbestos caused protracted and dose-responsive increases in proteins binding to nuclear NF-kappa B-binding DNA elements in hamster tracheal epithelial (HTE) cells. This binding was modulated by cellular glutathione levels. Antibodies recognizing p65 and p50 protein members of the NF-kappa B family revealed these proteins in two of the DNA complexes. Transient transfection assays with a construct containing six NF-kappa B-binding DNA consensus sites linked to a luciferase reporter gene indicated that asbestos induced …

Astrocytes Derived From P53-Deficient Mice Provide A Multistep In Vitro Model For Development Of Malignant Gliomas., Alan M. Yahanda Md, Facs, J M Bruner, L A Donehower, R S Morrison

PCI Publications and Projects

Loss or mutation of p53 is thought to be an early event in the malignant transformation of many human astrocytic tumors. To better understand the role of p53 in their growth and transformation, we developed a model employing cultured neonatal astrocytes derived from mice deficient in one (p53 +/-) or both (p53 -/-) p53 alleles, comparing them with wild-type (p53 +/+) cells. Studies of in vitro and in vivo growth and transformation were performed, and flow cytometry and karyotyping were used to correlate changes in growth with genomic instability. Early-passage (EP) p53 -/- astrocytes achieved higher saturation densities and had …

What's Happening: July 26, 1995, Maine Medical Center

What's Happening

No abstract provided.

Method For Inhibiting Angiogenesis With Aurintricarboxylic Acid, Its Analogues Or Salts, Delwood C. Collins, Antonio Gagliardi, Anjan Bhattacharyya

Obstetrics and Genecology Faculty Patents

Aurintricarboxylic acid has potent anti-angiogenic activity and may be used for new therapeutic approaches for diseases of neovascularization including the treatment of solid tumors, diabetic retinopathy and arthritis, among others.

What's Happening: July 12, 1995, Maine Medical Center

What's Happening

No abstract provided.

Prospects For The Control Of Bolivian Hemorrhagic Fever, Paul E. Kilgore, Clarence J. Peters, James N. Mills, Pierre E. Rollin, Lori Armstrong, Ali S. Khan, Thomas G. Ksiazek

Department of Pharmacy Practice

No abstract provided.

Update - July 1995, Loma Linda University Center For Christian Bioethics

Update

In this issue:

-- Gender Discrimination in the Medical Community

[ Responses to the American Medical Association of the Use of Anencephalic Neonates as Organ Donors ]
-- Maintain the "Dead Donor Rule"
-- Stairway to Hell
-- Let Parents Choose
-- Revisiting the Issues

Identification And Characterization Of Mitochondrial Dna Variants In Alzheimer's Disease, Natasha Singh Hamblet

Theses and Dissertations in Biomedical Sciences

Alzheimer's Disease (AD) is a complex neurodegenerative disorder that affects a significant portion of the human population regardless of ethnicity or gender. A mitochondrial hypothesis of AD has been proposed based on a number of studies which establish altered oxidative phosphorylation (OXPHOS) and ATP synthesis in AD tissue. ATP demand is most prevalent in the brain; damage to OXPHOS could severely impair brain metabolism, thereby leading to a decline in cognitive function. Four out of five complexes in the OXPHOS pathway are partly encoded by mitochondrial DNA (mtDNA); thus, this may be a crucial site of lesions that alter brain …

Immune Response To The Carcinoembryonic Antigen In Patients Treated With An Anti-Idiotype Antibody Vaccine, Kenneth A. Foon, Mala Chakraborty, William J. John, Amanda Sherratt, Heinz Köhler, Malaya Bhattacharya-Chatterjee

Markey Cancer Center Faculty Publications

We have generated an IgG1 murine monoclonal anti-idiotype antibody (Ab2) designated 3H1, which mimics a specific epitope on the carcinoembryonic antigen (CEA). Patients with CEA positive tumors are immunologically "tolerant" to CEA. We used 3H1 as a surrogate for CEA for vaccine therapy of 12 patients with advanced colorectal cancer. Each of the patients received a minimum of four intracutaneous injections of aluminum hydroxide precipitated 3H1 at either 1, 2, or 4 mg dosage per injection. 9 of 12 patients demonstrated anti-anti-idiotypic (Ab3) response to 3H1. All nine patients generated specific anti-CEA antibody demonstrated by reactivity with radiolabeled purified CEA; …

The Rat Tram Flap: A Human Analogue?, Geoffrey G. Hallock Md

Department of Surgery

No abstract provided.