Digital Commons Network^™

The Effect Of Jogging On Anterior Knee Laxity After Anterior Cruciate Ligament Reconstruction, Fawzi F. Bouzubar

Rehabilitation Sciences Theses & Dissertations

Jogging is commonly performed in the latter stages of the rehabilitation program (2½-3 months) following ACL reconstruction. However, its cyclic loading effect on the ACL graft, 4-6 months after surgery has not been examined. Therefore, the purpose of this study was to examine changes in anterior knee laxity before and after 15 minutes of jogging on the treadmill. Subjects (N=l 1) were 8 males and 3 females (X age= 30.27 ± 4.76 years; heights= 175.7 ± 10.7 cm; weights= 81.1 ± 15.9 kg) with unilateral ACL reconstruction (central 1/3 patellar tendon graft, postoperation range = 4-6 months). Measurements were made …

A Serological Survey Of Equine Infectious Diseases In Thailand, Urasri Tantaswasdi, Ruenrudee Punyahotra, Surapong Wongkasemjit, Patchima Indrakamhang

The Thai Journal of Veterinary Medicine

During 1997-1998, a serological survey of equine infectious diseases in Thailand was undertaken. Blood, sera and blood smears were taken from 400 horses, donkeys and mules out of an equine population of 8,600. Tests were carried out at the National Institute of Animal Health, Bangkok, Thailand, and at the USDA laboratories, USA, for 10 diseases. There was no evidence of the presence of African Horse Sickness, Glanders, Dourine, Surra and Equine Viral Arteritis. There was one reactor to Babesia caballi and 62 to Babesia equi. Fourteen reactors to equine influenza virus subtype 1 (Praque) and 24 to subtype 2 (Newmarket …

Increased Glutathione Metabolic Defense Capabilities In Cultured Alzheimer's Diseased Lymphoblast Cell Lines, Collin M. Shaw

Dissertations and Theses

The hypothesis to be tested states that the pathology of Alzheimer's disease (AD) involves elevated levels of oxidative stress, resulting in elevated levels of cellular oxidative defense mechanisms. If the premise is true, than AD pathologically afflicted cells should have a higher demand for glutathione (GSH) as an innate oxidative defense mechanism hence; greater GSH concentrations, increased GSH resynthesis capabilities, and increased levels of cystathionine gamma-lyase (CNase). Alzheimer diseased and age matched control lymphoblast cells, obtained from OHSU's Oregon Brain Aging Study, were cultured, and GSH biochemistry was subsequently evaluated. GSH was depleted by exposing cells to the GSH depleting …

Lobeline Compounds As A Treatment For Psychostimulant Abuse And Withdrawal, And For Eating Disorders, Peter A. Crooks, Linda P. Dwoskin

Pharmaceutical Sciences Faculty Patents

Methods are disclosed that suggest the use of lobeline and analogs thereof in treating individuals for drug dependence and withdrawal and for eating disorders.

Trophectoderm Differentiation In The Bovine Embryo: Characterization Of A Polarized Epithelium., L C Barcroft, A Hay-Schmidt, A Caveney, E Gilfoyle, E W Overstrom, P Hyttel, A J Watson

Obstetrics & Gynaecology Publications

Blastocytst formation is dependent on the differentiation of a transporting epithelium, the trophectoderm, which is coordinated by the embryonic expression and cell adhesive properties of E-cadherin. The trophectoderm shares differentiative characteristics with all epithelial tissues, including E-cadherin-mediated cell adhesion, tight junction formation, and polarized distribution of intramembrane proteins, including the Na-K ATPase. The present study was conducted to characterize the mRNA expression and distribution of polypeptides encoding E-cadherin, beta-catenin, and the tight junction associated protein, zonula occludens protein 1, in pre-attachment bovine embryos, in vitro. Immunocytochemistry and gene specific reverse transcription--polymerase chain reaction methods were used. Transcripts for E-cadherin and …

Staphylococcal Enterotoxin B Primes Cytokine Secretion And Lytic Activity In Response To Native Bacterial Antigens, K. M. Mason, T. D. Dryden, Nancy J. Bigley, P. S. Fink

Neuroscience, Cell Biology & Physiology Faculty Publications

Superantigens stimulate T-lymphocyte proliferation and cytokine production, but the effects of superantigen exposure on cell function within a complex, highly regulated immune response remain to be determined. In this study, we demonstrate that superantigen exposure significantly alters the murine host response to bacterial antigens in an in vitro coculture system. Two days after exposure to the superantigen staphylococcal enterotoxin B, splenocytes cultured with Streptococcus mutans produced significantly greater amounts of gamma interferon (IFN-gamma) and interleukin-12 than did sham-injected controls. The majority of IFN-gamma production appeared to be CD8(+) T-cell derived since depletion of this cell type dramatically reduced the levels …

The Meeting Of Pain And Depression: Comorbidity In Women, Marta Meana

Psychology Faculty Research

The higher prevalence of depression in women is coupled with a higher prevalence of pain complaints. Growing evidence suggests that the comorbidity of these conditions is also proportionately higher in women than men. This paper critically reviews the empirical findings relating to gender differences in comorbid pain and depression as well as findings in support of hypothesized etiologic factors that could explain why women may be more susceptible than men to comorbidity. The empirical evidence for biogenic, psychogenic, and sociogenic explanatory models is presented, and an integration of these models is proposed as a guideline to both research and clinical …

Chimeric Isoprenoid Synthases And Uses Thereof, Joseph Chappell, Kyoungwhan Back

Pharmaceutical Sciences Faculty Patents

Disclosed is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous isoprenoid synthase. Also disclosed is a chimeric isoprenoid synthase polypeptide including an assymetrically positioned homologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring …

Bird Use Of Riparian Vegetation Along The Truckee River, Califonia And Nevada, Suellen Lynn, Michael L. Morrison, Amy J. Kuenzi, Jennifer C. C. Neale, Benjamin N. Sacks, Robin Hamlin, Linnea S. Hall

Great Basin Naturalist

The Truckee River in California and Nevada is subject to diverse water regimes and a corresponding variety of flow rates. Original riparian vegetation has been altered by these variable flow rates and by a variety of human uses resulting in loss of native riparian vegetation from its historic extent. We conducted bird surveys along the Truckee River during spring 1993 to (1) determine relationships between birds and the present vegetation; (2) determine the importance of different vegetation types to sensitive bird species that have declined recently in the western United States due to competition from exotic plant species, cowbird ( …

Thiazolidinediones Inhibit Lipoprotein Lipase Activity In Adipocytes, Subramanian Ranganathan, Philip A. Kern

Clinical and Translational Science Faculty Publications

The thiazolidinediones troglitazone and BRL 49653 improve insulin sensitivity in humans and animals with insulin resistance. Adipose tissue lipoprotein lipase is an insulin-sensitive enzyme. We examined the effects of thiazolidinediones on lipoprotein lipase expression in adipocytes. When added to 3T3-F442A, 3T3-L1, and rat adipocytes in culture, troglitazone and BRL 49653 inhibited lipoprotein lipase activity. This inhibition was observed at concentrations as low as 0.1 μM and within 2 h after addition of the drug. Lipoprotein lipase activity was inhibited in differentiated adipocytes as well as the differentiating cells. Despite this decrease in enzyme activity, these drugs increased mRNA levels in …

Update - October 1998, Loma Linda University Center For Christian Bioethics

Update

In this issue:

-- The Practice of the Ministry of Medicine

Assessment Of Side Effects Induced By Injection Of Different Adjuvant/Antigen Combinations In Rabbits And Mice, P.P.A.M. Leenaars, M. A. Koedam, P. W. Wester, V. Baumans, E. Claassen, C. F.M. Hendriksen

Biomedicine and Animal Models in Research Collection

We evaluated the side effects induced by injection of Freund's adjuvant (FA)and alternative adjuvants combined with different antigens. Rabbits and mice were injected subcutaneously, intramuscularly (rabbits) and intraperitoneally (mice)with different adjuvants (FA, Specol, RIBI,TiterMax, Montanide ISA50)in combination with several types of antigens (synthetic peptides, autoantigen, glycolipid, protein, mycoplasma or viruses). The effects of treatment on the animals' well-being were assessed by clinical and behavioural changes (POTand LABORASassays) and gross and histopathological changes. In rabbits, treatment did not appear to induce acute or prolonged pain and distress. Mice showed behavioural changes immediately after (predominantly secondary) immunization. Injection of several adjuvant/antigen mixtures …

Animal Organs In Humans: Uncalculated Risks And Unanswered Questions, Gillian R. Langley, Joyce D'Silva

Biomedicine and Animal Models in Research Collection

This report, produced jointly by the British Union for the Abolition of Vivisection and Compassion in World Farming, fills a number of significant gaps in the current debate about xenotransplantation.

In this report we also summarise the ethical and welfare issues concerning experiments on animals for xenotransplant research and their possible use as source animals for organs. Both these aspects are responsible for much pain and distress caused to many animals. We prefer the term “source animals” to “donor animals”, because animals do not choose to donate their organs for xenotransplantation.

Transient Expression Of A Translation Initiation Factor Is Conservatively Associated With Embryonic Gene Activation In Murine And Bovine Embryos., P A De Sousa, A J Watson, R M Schultz

Obstetrics & Gynaecology Publications

In the present study the abundance of mRNAs for eukaryotic translation initiation factors eIF-1A (formerly known as eIF-4C), -2alpha, -4A, -4E, and -5 was examined in in vivo-derived mouse embryos throughout preimplantation development using a semiquantitative reverse transcription-polymerase chain reaction assay. Although the mRNA profile for each gene is unique, only mRNA for eIF-1A transiently increases during embryonic gene activation (EGA) at the 2-cell stage, and this was confirmed by an independent hybridization-based assay. In in vitro-developed bovine embryos, mRNA for eIF-1A was transiently detected at the 8-cell stage, when the major activation of the genome occurs in this species. …

Influence Of Physical Setting On Seagrass Landscapes Near Beaufort, North Carolina, Usa, Mark S. Fonseca, Susan S. Bell

Integrative Biology Faculty and Staff Publications

Field surveys were conducted in Core and Back Sounds, North Carolina, USA, to relate the physical setting of seagrass beds, as measured by a wave exposure index (REI), tidal current speed and water depth, to various measures of the sedimentary environment, spatial heterogeneity of seagrass distribution and measures of seagrass abundance. Seagrass beds in this area form patterns ranging from continuous to semi-continuous to widely dispersed, discrete patches across a gradient of increasing hydrodynamic activity. Tidal current speeds, exposure to waves and relative water depths revealed strong correlative evidence that physical processes influenced landscape-scale (50 x 50 m range with …

Pathogenicity Of An Enterotoxigenic Escherichia Coli Hemolysin (Hlya) Mutant In Gnotobiotic Piglets, Rodney A. Moxley, Emil M. Berberov, David H. Francis, Jun Xing, Mahtab Moayeri, Rodney A. Welsch, Diane R. Baker, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Pigs infected with hemolytic F4⁺ strains of enterotoxigenic Escherichia coli often develop septicemia secondary to intestinal infection. We tested the hypothesis that inactivation of hemolysin would reduce the ability of F4⁺ enterotoxigenic E. coli to cause septicemia in swine following oral inoculation. Inactivation of the hemolysin structural gene (hlyA) did not decrease the incidence of septicemia in the gnotobiotic piglet model.

Stachybotrys: Is Nevada At Risk?, Terrylynn C. Foley

UNLV Theses, Dissertations, Professional Papers, and Capstones

Stachybotrys chartarum (atra) is a toxigenic fungus that is known to adversely impact the health of animals. Until recently, there have only been a few documented clinical reports linking Stachybotrys chartarum to human health effects. Scientists are increasingly convinced that Stachybotrys and its metabolites are responsible for several adverse health effects experienced by people all over the world, although conclusive proof has yet to be established. All the physical requirements needed for indoor Stachybotrys sporulation and growth is provided through water leaks (plumbing, roof or ceiling), flood events, nutrient sources (cellulose and nitrogen), pH, and temperature(23° - 28°C). Stachybotrys has …

Chironian Fall/Winter 1998, New York Medical College

The Chironian

No abstract provided.

Jefferson Alumni Bulletin – Volume Xlvii, Number 4, October 1998

The Bulletin (formerly the Jefferson Medical College Alumni Bulletin)

Jefferson Alumni Bulletin – Volume XLVII, Number 4, October 1998

Flanagan, Gallo, and Nadel are Honored with the Alumni Achievement Awards, Page 6

Clinic Presentations Treat a Range of Fields, Page 8

Alumni are Invited to Compete for the Gratz Prize, Page 13

Annual Giving Tops Two Million Dollars, Page 26

A Protein Encoded By The Latency-Related Gene Of Bovine Herpesvirus 1 Is Expressed In Trigeminal Ganglionic Neurons Of Latently Infected Cattle And Interacts With Cyclin-Dependent Kinase 2 During Productive Infection, Yunquan Jiang, Ashfaque Hossain, Mariah Teresa Winkler, Todd Holt, Alan R. Doster, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Despite productive viral gene expression in the peripheral nervous system during acute infection, the bovine herpesvirus 1 (BHV-1) infection cycle is blocked in sensory ganglionic neurons and consequently latency is established. The only abundant viral transcript expressed during latency is the latency-related (LR) RNA. LR gene products inhibit S-phase entry, and binding of the LR protein (LRP) to cyclin A was hypothesized to block cell cycle progression. This study demonstrates LRP is a nuclear protein which is expressed in neurons of latently infected cattle. Affinity chromatography indicated that LRP interacts with cyclin-dependent kinase 2 (cdk2)-cyclin complexes or cdc2-cyclin complexes in …

A Cross-Sectional Study Examining Lifestyle And Rheumatoid Arthritis, Leslie Nazaroff

Loma Linda University Electronic Theses, Dissertations & Projects

Objective. Rheumatoid arthritis (RA) is a chronic inflammatory disease that affects the synovial joints. RA affects 0.5-2% of the population. Though it is predominantly a progressive disease, adjunct therapy may slow the destructive pathway or alleviate affiliated symptoms. This study examined the effects of lifestyle (diet and exercise) and self-efficacy on symptoms of RA as measured by disease activity and health satisfaction.

Methods. Seventy-five RA patients (77% female) from the Loma Linda University Faculty Medical Offices, Rheumatology Department completed self-assessment surveys on their lifestyle practices and RA affliction. Disease symptoms and activity were assessed with the Arthritis Impact Measurement …

What's Happening: September 3, 1998, Maine Medical Center

What's Happening

No abstract provided.

Dentalgram - Vol. 12, No. 09, Loma Linda University School Of Dentistry

Dentalgram

In this issue:

• Students dentists eXite 2,000 attendees
• Firstand report of Sudan bombing
• Dr. Crawford visits Africa
• Student news
• Department news
• Research studies
• Fall Family Retreat
• CDE tops in nation
• Dr. Heisler visits Alaska
• Lee family visits Guam
• Faculty Dental Offices Open House
• Fall Classic Picnic

Optimization Of Solid-State Fermentation Parameters For The Production Of Xylanase By Trichoderma Longibrachiatum On Wheat Bran, Elizabeth R. Ridder, Sue E. Nokes, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

Solid-state fermentation has the potential to produce inexpensive enzymes for use in high-volume industrial applications. Process parameters such as substrate moisture content and length of fermentation can have a significant effect on the amount and timing of enzyme production. This study was conducted in two stages, a screening stage and an optimization stage, to determine the effects of moisture content of the substrate, surfactant addition upon inoculation, depth of the substrate, and duration of fermentation on xylanase activity produced by Trichoderma longibrachiatum. Screening fermentations were conducted at 25°C, 50 and 75% wet basis moisture content (w.b.), 0.0 and 0.2% …

Health Care Surrogate Statutes: Ethics Pitfalls Threaten The Interests Of Incompetent Patients, Aaron N. Krupp

West Virginia Law Review

No abstract provided.

Localization Of Sodium, Potassium-Adenosine Triphosphatase In The Cerebral Cortex Of Developing Rats, Maki Ujiie

Loma Linda University Electronic Theses, Dissertations & Projects

Active transport via Na⁺, K⁺-ATPase plays an important role in establishing and maintaining proper ionic gradient across all mammalian cell membranes. A strict regulation of Na⁺, and K⁺ ion levels is especially important in excitable cells such as neurons and myocytes. Inhibition of the enzyme is associated with several pathological conditions and has serious detrimental effects on cerebral cortical cells. Yet the enzyme appears to be limited in amount and distribution in newborn rats. In this study, Na_⁺, K⁺- ATPase expression and distribution in early postnatal rats were studied …

Alternative Splicing Of The Latency-Related Transcript Of Bovine Herpesvirus 1 Yields Rnas Containing Unique Open Reading Frames, Laxminarayana R. Devireddy, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

The latency-related transcript (LRT) of bovine herpesvirus 1 (BHV-1) is the only abundant viral RNA detected during latency. A previous study (A. Hossain, L. M. Schang, and C. Jones, J. Virol. 69:5345–5352, 1995) concluded that splicing of polyadenylated [poly(A) ⁺] and splicing of nonpolyadenylated [poly(A) ^-] LRT are different. In this study, splice junction sites of LRT were identified. In trigeminal ganglia of acutely infected calves (1, 7, or 15 days postinfection [p.i.]) or in latently infected calves (60 days p.i.), alternative splicing of poly(A) ⁺ LRT occurred. Productive viral gene expression in trigeminal ganglia is readily detected …

Regulation Of Connexin43 By Phosphorylation In Heart Development, John C. Duncan

Loma Linda University Electronic Theses, Dissertations & Projects

Serine/threonine protein kinases have been shown to reversibly regulate cell-cell communication through gap junctions formed by connexin 43 (Cx43) (Godwin et al.7 1993). The Cx43 protein expression levels are unclear and what role phosphorylation plays in the regulation of signal transduction through these gap junctions during heart formation. Reportedly, mutations which replace potentially phosphorylatable serines within the Cx43 protein are associated with heart malformations (Britz-Cunningham et al.. 1995), highlighting the importance of determining the levels of Cx43 and the activities of PKA and PKC during heart development.

Western analysis performed on hearts from developmentally significant time points revealed principally high …

What's Happening: August 25, 1998, Maine Medical Center

What's Happening

No abstract provided.

What's Happening: August 6, 1998, Maine Medical Center

What's Happening

No abstract provided.