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The Changes Of Femoral Artery Harmonics In Hypoxia：An Animal Study, Tyng-Guey Wang, Yio-Wha Shau, Jeng-Yi Shieh, Juey-Jen Hwang, Chung-Li Wang

Rehabilitation Practice and Science

Using the non-invasive method to monitor the pulmonary artery pressure in the exercise training program of the chronic obstructive pulmonary disease patients might reduce the incidence of cardiac complication from the pulmonary hypertension and hypoxia. The purpose of this study was to detect the femoral artery pulse pressure and its harmonic changes of the rabbits in the hypoxia status.Ten rabbits underwent the femoral artery catherization for detecting their femoral artery pressure and their harmonics. Ten percent carbon dioxide mixed gas was inhaled to reduce the oxygen saturation of the rabbits from 95 % to 90% or lower. The mean femoral …

Trypanosome Infection Rate In Cattle At Nguruman, Kenya, R. A. Tarimo-Nesbitt, T. K. Golder, R. D. Dransfield, M. F. Chaudhury, R. Brightwell

All Peer-Reviewed Publications

Trypanosome infection rate in cattle at Nguruman was investigated in a study conducted in 1984-1986. Shifting pastoralism significantly reduced trypanosome infections in cattle. The cattle were more heavily infected with Trypanosoma congolense (16.5%) than Trypanosoma vivax (4.95%) and Trypanosoma brucei (0.19%). Trypanosoma theileri was observed only once among the cattle examined. Mixed trypanosome infections in cattle were observed to be 2.75% and 0.014% for T. congolense/T. vivax and T. congolense/T. brucei, respectively. The duration of infection in the cattle was 55 days for T. congolense and 79 days for T. vivax. High infections in cattle were observed 2 months after …

Technique For Specifying The Fatty Acid At The Sn2 Position Of Acylglycerol Lipids, Robert C. Dickson, Robert L. Lester, M. Marek Nagiec

Molecular and Cellular Biochemistry Faculty Patents

A method for specifying a fatty acid at the sn2 position of acylglycerol lipids including (a) transfecting a vector including the SLC1 gene or a variant thereof into embryonic biological material, and (b) allowing the SLC1 gene to specify the type of fatty acid at the sn2 position of acylglycerol lipids. Also provided for is an isolated SLC1 gene and a probe for its detection.

Nucleotide Sequences For Detection Of Serpulina Hyodysenteriae, Gerald E. Duhamel, Robert Elder

School of Veterinary and Biomedical Sciences: Faculty Publications

The invention provides a method for detecting the presence of Serpulina hyodysenteriae in a biological Sample, an oligonucleotide primer and an S. hyodysenteriae-specific oligonucleotide probe useful in that method, and an article of manufacture that contains the primers and/or probe. Also provided are an about 2.3-kb DNA fragment derived from genomic DNA of S. hyodysenteriae and encoding for an about 56 kDa polypeptide, a recombinant expression vector containing the DNA fragment, the 56 kDa polypeptide and a monoclonal antibody reactive with the peptide, and a method of assaying for antibodies reactive with the 56 kDa peptide.

Induction Of Integral Membrane Pam Expression In Att-20 Cells Alters The Storage And Trafficking Of Pomc And Pc1, Giuseppe D. Ciccotosto, Martin R. Schiller, Betty A. Eipper, Richard E. Mains

Life Sciences Faculty Research

Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides. The bifunctional PAM protein contains an NH2-terminal monooxygenase (PHM) domain followed by a lyase (PAL) domain and a transmembrane domain. The cytosolic tail of PAM interacts with proteins that can affect cytoskeletal organization. A reverse tetracycline-regulated inducible expression system was used to construct an AtT-20 corticotrope cell line capable of inducible PAM-1 expression. Upon induction, cells displayed a time- and dose-dependent increase in enzyme activity, PAM mRNA, and protein. Induction of increased PAM-1 expression produced graded changes in PAM-1 metabolism. Increased expression of …

From Humanae Vitae To Donum Vitae: Symmetry And Consistency In Catholic Biomedical Teaching, Paul F. Deladurantaye

The Linacre Quarterly

No abstract provided.

Fas-Fasl Interaction Involved In Pathogenesis Of Ocular Toxoplasmosis In Mice, Mark S. Hu, Joseph D. Schwartzman, Grant R. Yeaman, Jane Collins, Rosanne Seguin, Imtiaz A. Khan, Lloyd H. Kasper

Dartmouth Scholarship

Ocular toxoplasmosis is a potentially blinding intraocular inflammation. The intent of this study was to investigate the role of Fas-FasL interaction in a murine model of acquired ocular toxoplasmosis induced by intracameral inoculation of Toxoplasma gondii. Intraocular inflammation, Fas and FasL expression on lymphocytes and on ocular tissues, the occurrence of apoptosis, and the frequency of CD8⁺ and CD4⁺ T cells in the infected eyes were analyzed in C57BL/6 (B6) mice. Susceptibility to parasite-induced intraocular inflammation was observed in Fas-deficient (B6-lpr) and FasL-deficient (B6-gld) mice. Inoculation of 5,000 T. gondii tachyzoites induced significant …

Development Of A Firefly Luciferase-Based Assay For Determining Antimicrobial Susceptibility Of Mycobacterium Avium Subsp. Paratuberculosis, Stephanie L. Williams, N. Beth Harris, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Paratuberculosis (Johne’s disease) is a fatal disease of ruminants for which no effective treatment is available. Presently, no drugs against Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis), the causative agent of Johne’s disease, are approved for use in livestock. Additionally, M. paratuberculosis has been linked to a human chronic granulomatous ileitis (Crohn’s disease). To assist in the evaluation of antimicrobial agents with potential activity against M. paratuberculosis, we have developed a firefly luciferase-based assay for the determination of drug susceptibilities. The microorganism used was M. paratuberculosis K-10(pYUB180), a clinical isolate carrying a plasmid with the firefly luciferase gene. …

Bovine Tuberculosis Eradication: Uniform Methods And Rules, Effective January 22, 1999

Michigan Bovine Tuberculosis Bibliography and Database

These Uniform Methods and Rules (UMR) are the minimum standards adopted and approved by the Deputy Administrator, Veterinary Services (VS), Animal and Plant Health Inspection Service (APHIS), on January 21, 1999. They were established for the maintenance of tuberculosis-free accredited herds of cattle, captive cervids, bison, and goats, and the maintenance of State status in the U.S. Department of Agriculture’s tuberculosis eradication program.

These minimum standards do not preclude the adoption of more stringent standards by any State, status zone within a State, or region containing multiple States.

Antiretroviral Cytolytic T-Lymphocyte Nonresponsiveness: Fasl/Fas-Mediated Inhibition Of Cd4+ And Cd8+ Antiviral T Cells By Viral Antigen-Positive Veto Cells, Robert F. Rich, William R. Green

Dartmouth Scholarship

C57BL/6 (H-2^b ) mice generate type-specific cytolytic T-lymphocyte (CTL) responses to an immunodominant K^b-restricted epitope, KSPWFTTL located in the membrane-spanning domain of p15TM of AKR/Gross murine leukemia viruses (MuLV). AKR.H-2^b congenic mice, although carrying the responder H-2^b major histocompatibility complex (MHC) haplotype, are low responders or nonresponders for AKR/Gross MuLV-specific CTL, apparently due to the presence of inhibitory AKR.H-2^b cells. Despite their expression of viral antigens and K^b, untreated viable AKR.H-2^b spleen cells cause dramatic inhibition of the C57BL/6 (B6) antiviral CTL response to in vitro stimulation with AKR/Gross MuLV-induced …

In The Affirmative, Vol.6, No.1 (Mid-January/Mid-February 1999), Mike Martin, The Aids Project

In the affirmative (1993-1999)

No abstract provided.

Locomotion And Conditioned Place Preference Produced By Acute Intravenous Amphetamine: Role Of Dopamine Receptors And Individual Differences In Amphetamine Self-Administration, M. T. Bardo, J. M. Valone, Rick A. Bevins

Department of Psychology: Faculty Publications

Although previous studies have shown that dopamine (DA) antagonists block amphetamine reward, these studies have utilized animal models that involve repeated exposures to amphetamine. The present investigation examined the effect of DA antagonists on single-trial conditioned place preference (CPP) produced by acute intravenous (IV) amphetamine in rats. In the first experiment, rats were prepared with a jugular catheter and then received an acute IV injection of amphetamine (0.1–3 mg/kg) paired with one compartment of a CPP apparatus. Relative to sham controls (no IV catheter), amphetamine produced a dose-dependent increase in locomotor activity and CPP. Two further experiments demonstrated that both …

Transgenic Mice Which Overexpress Neurotrophin-3 (Nt-3) And Method Of Use, Kathryn M. Albers, Brian M. Davis

Neuroscience Faculty Patents

Transgenic mice express increased levels of neurotrophin-3 (NT-3) in epithelium when their ancestors are microinjected with the NT-3 gene. The NT-3 growth factor expressing transgenic mice are useful in the study of neurodegenerative disorders of the brain such as Parkinson's syndrome and Alzheimer's disease, of the spinal cord motor neurons such as amyotrophic lateral sclerosis, and for testing drug candidates for the treatment of these diseases.

Canavanine Analogs And Their Use As Chemotherapeutic Agents, Peter A. Crooks, Gerald A. Rosenthal

Pharmaceutical Sciences Faculty Patents

This invention relates to canvanine analogs, their pharmaceutical compositions, and a method for treatment of cancer, particularly pancreatic cancer.

What's Happening: January 6, 1999, Maine Medical Center

What's Happening

No abstract provided.

Housing And Population Health: A Review Of The Literature, Stephen Hwang, Esme Fuller-Thomson, J. David Hurlchanski, Toba Bryant, Youssef Habib, Wendy C. Regoeczi

Sociology & Criminology Faculty Publications

Research into the relationship between housing and health has frequently been narrowly focused, fragmented, and of marginal practical relevance to either housing or health studies. Population health research, in its reference to the importance of the social and physical environment, rarely mentions housing. While it has been recognized for some time that there is a need for the development of a co-ordinated, integrated and cumulative body of housing and health research, there is still very little formal co-ordination between housing policy and population health policy. More attention needs to be paid to measuring the nature and extent to which better …

Interaction Of Laminin Beta2 With Eps8 And Desmin In Muscle Cells, Luke Xueliang Cui

Theses, Dissertations and Capstones

Laminin β2 is localized to the synaptic basal lamina (BL) in muscle and glomerular BL in kidney. To find interacting proteins, a mouse kidney cDNA library was screened with domain I of rat laminin β2 using the yeast two-hybrid system. Fifteen positive clones were found. The DNA sequence of three each of these clones corresponded to the phosphoprotein Eps8 (epidermal growth factor receptor pathway substrate clone 8, which is tyrosine phosphorylated after EGF stimulation) and the intermediate filament (IF) protein keratin. To determine if this interaction occurred in muscle cells, C2 cells transfected with the rat …

Carboxy-Terminal Conversion Of Profibrillin To Fibrillin At A Basic Site By Pace/Furin-Like Activity Required For Incorporation In The Matrix, Michael Raghunath, Elizabeth A. Putnam, Timothy Ritty, Daniel Hamstra, Eun-Sook Park, Mathias Tschödrich-Rotter, Reiner Peters, Alnawaz Rehemtulla, Dianna M. Milewicz

Public and Community Health Sciences Faculty Publications

Fibrillin-1, the main component of 10-12 nm microfibrils of the extracellular matrix, is synthesized as profibrillin and proteolytically processed to fibrillin. The putative cleavage site has been mapped to the carboxy-terminal domain of profibrillin-1, between amino acids arginine 2731 and serine 2732, by a spontaneous mutation in this recognition site that prevents profibrillin conversion. This site contains a basic amino acid recognition sequence (R-G-R-K-R-R) for proprotein convertases of the furin/PACE family. In this study, we use a mini-profibrillin protein to confirm the cleavage in the carboxy-terminal domain by both fibroblasts and recombinantly expressed furin/PACE, PACE4, PC1/3 and PC2. Site-directed mutagenesis …

The Study On Morphological And Physiological Characters Affecting The Productivity Of Some Honey Bee (Apis Mellifera L.) Genotypes Of Turkey, Ahmet Güler

Turkish Journal of Veterinary & Animal Sciences

Experimental material consisted of 36 selected colonies collected from 6 different regions of Turkey. During the swarming season 20 worker bees were taken from each colony in order to measure the morphological characters including corbicul and wax mirror area, tongue length and body size. Development of the colony population, brood rearing activity and honey yield during a season were determined and the hive was weighed weekly for 6 months under migratory beekeeping conditions. The relationships between some morphological and physiological characters which affected the productivity of genotypes were investigated. The average honey yields were found to be 24.38±3.58, 26.57±5.51, 57.15±3.43, …

Role Of Vitamin E In Decreasing The Toxic Effect Of Digoxin, İsmai̇l Meral, Burhanetti̇n Baydaş, Fahri̇ Bayiroğlu, Hamdi̇ Uysal

Turkish Journal of Veterinary & Animal Sciences

This experiment was carried out to evaluate the role of vitamin E in decreasing the toxic effect of digoxin. Electrocardiographic recordings of New Zealand rabbits which were treated with digoxin-only or digoxin+vitamin E were used. Recordings were obtained before and 24 hours after treatments. It was found that digoxin-only treatment caused a sinusoidal tachicardia without changing the durations or amplitudes of any wave on the trace. However, digoxin+vitamin E treatment did not produce any increase in heart rate. It was concluded that vitamin E injection along with digoxin injection might lower the toxic effect of digoxin. More studies are needed …

A Comparison Study Of Food Facility Inspection Scores And Consumer Complaints, Debora Kim Leuer

Theses Digitization Project

No abstract provided.

Anxiety And Depression: An Empirical Investigation Of The Diathesis-Stress Model Of Psychopathology, Deborah Jean Hartley

Theses Digitization Project

No abstract provided.

The Use Of Art Therapy In Counseling Children, Laura Longo

Graduate Research Papers

Art is being used more and more by counselors working with children, particularly within the school setting because it allows for children to communicate nonverbally in a timely manner. "Art therapy in a school setting can offer children the opportunity to work through obstacles that are impeding their educational progress. It can facilitate appropriate social behaviors and promote healthy affective development" (Bush, 1997, p. 16).

The purpose of this paper is to explore how art is used in counseling children and examine specific techniques. Also, the author will focus on the stages of artistic development of children, the use of …

Dissociative Identity Disorder : Features, Etiology, And Treatment, Gretchen Elizabeth Honsell

Graduate Research Papers

Dissociative Identity Disorder appears to be diagnosed more frequently in the current clinical arena. This may be connected to increased awareness of how people respond and cope with traumatic events, both singular and prolonged or serial. This increase in diagnoses may also correspond with new associations between childhood abuse and trauma, as well as research into how trauma is coded in memory.

Dissociation can be viewed as a natural phenomena that, when overly utilized as a defense against trauma and its impact, may develop in some persons into DID. Treatment of DID tends to progress through four phases: initial, middle, …

Identifying The Autoantigen Of A Diabetogenic Cd8 T Cell Clone Isolated From Young Nod Mice, Caroline R. Dumont

Yale Medicine Thesis Digital Library

No abstract provided.

Un Modele D'Autisme Chez Le Cobaye [A Guinea Pig Model Of Autism], J. Caston, Daniel Mellier, Henry Godfrey, N. Delhaye-Bouchaud, Jean Mariani

NYMC Faculty Publications

No abstract provided.

Research Fundamentals: V. The Use Of Laboratory Animal Models In Research, Brian J. O'Neil, Jeffrey A. Kline, Keith Burkhart, John Younger

Biomedicine and Animal Models in Research Collection

Animal research has provided important information about many aspects of the pathophysiology of human disease. Well-performed animal studies can determine the potential benefit of many proposed therapeutic interventions, and experimental results from animal studies have served as the basis for many landmark clinical trials. Many animal research models are described in the research literature, and choosing the appropriate model to answer a research question can be a daunting task. Even more challenging is developing a new model when none of the existing systems are relevant to the proposed question. This article was prepared by members of the SAEM Research Committee …

A Comparison Of The Maximal Torque Production Of The Quadriceps Muscle During Morning And Afternoon Strength Assessment, Michael Rexin

Physical Therapy Scholarly Projects

The purpose of this study is to compare maximal quadriceps peak torque production in isokinetic strength assessment in the morning and the afternoon. Thn1y male subjects between the ages of21 and 43 volunteered for this study. The subjects were randomly assigned to either an early morning group (7:30 AM to 9 AM) or an afternoon group (2:30 PM to 5 PM). Each subject performed six maximal concentric isokinetic contractions with the quadriceps muscles after a warm up and an initial familiarization session. Data analysis was done using a t-test for paired samples and Pearson's correlation The results showed a .90 …

Common Parasites, Diseases And Injuries Of Freshwater Fishes In The Northwest Territories And Nunavut, D. B. Stewart, L. M. J. Bernier

Zoonotics and Wildlife Disease: Publications

This booklet gives basic information on common parasites, diseases and injuries of fish harvested from fresh water in the Northwest Territories and Nunavut (Canada). It answers some of the more common questions asked by Aboriginal, domestic, sport and commercial harvesters, and by fisheries researchers. It is not an in depth treatment of fish health. Parasites that are uncommon or too small to be seen with the naked eye and diseases that are poorly known are not discussed. Relatively little research has been conducted on fish parasites in the region, less still on fish diseases and injuries.

The Human Ehrlichioses In The United States, Jennifer H. Mcquiston, Christopher D. Paddock, Robert C. Holman, James E. Childs

Zoonotics and Wildlife Disease: Publications

The emerging tick-borne zoonoses human monocytic ehrlichiosis (HME) and human granulocytic ehrlichiosis (HGE) are underreported in the United States. From 1986 through 1997, 1,223 cases (742 HME, 449 HGE, and 32 not ascribed to a specific ehrlichial agent) were reported by state health departments. HME was most commonly reported from southeastern and southcentral states, while HGE was most often reported from northeastern and upper midwestern states. The annual number of reported cases increased sharply, from 69 in 1994 to 364 in 1997, coincident with an increase in the number of states making these conditions notifiable. From 1986 through 1997, 827 …