Digital Commons Network^™

Inflammatory Pain In Mice Induces Light Cycle-Dependent Effects On Sleep Architecture, Dominika J Burek, Khairunisa Mohamad Ibrahim, Andrew G Hall, Ashish Sharma, Jessica A Cucinello-Ragland, Erik S Musiek, Jose A Morón, William A Carlezon Jr

2020-Current year OA Pubs

Pain syndromes include physical, sensory, emotional, and cognitive symptoms such as disability, negative affect, feelings of stress, and fatigue. Experimental induction of long-term inflammatory pain in rodents by hindpaw injection of complete Freund's adjuvant (CFA) produces anhedonia and dysregulated naturalistic behaviors, similar to the effects of unregulated stress. We examined whether these similarities extend to changes in sleep and rhythms, such as those induced by chronic social defeat stress, using actigraphy and wireless EEG in mice. Comparisons were made between groups that received injections at the onset of the light or dark phase. We found that CFA-induced inflammatory pain alters …

Dual-Vector Raavrh8 Gene Therapy For Gm2 Gangliosidosis: A Phase 1/2 Trial, Florian Eichler, Xuntian Jiang, Et Al.

2020-Current year OA Pubs

The dual rAAVrh8-HEXA and rAAVrh8-HEXB vector can restore central nervous system hexosaminidase (Hex) enzyme activity, decrease GM2 levels in cerebrospinal fluid and rescue phenotypic consequences of GM2 gangliosidosis, Tay-Sachs and Sandhoff diseases in animal models following simultaneous bi-thalamic (BiT) injections. Following up on an n = 2 expanded access trial, we initiated a phase 1/2, single-dose, dose-escalation of combined BiT, intra-cisterna magna and intrathecal infusion in children with Tay-Sachs and Sandhoff diseases (six infantile, three juvenile). The BiT injection volume and vector dose were doubled between four cohorts, with the lowest dose matching the earlier expanded access trial. Cerebrospinal fluid …

Bacterial Vaginosis Toxins Impair Sperm Capacitation And Fertilization, Shweta Bhagwat, Leila Asadi, Ronald Mccarthy, Juan Ferreira, Ping Li, Ethan Li, Sariela Spivak, Ariana Gaydon, Vaka Reddy, Christy Armstrong, Sydney R Morrill, Hillary Zhou, Amanda L Lewis, Warren G Lewis, Celia M Santi

2020-Current year OA Pubs

STUDY QUESTION: What effect do toxins produced by bacterial vaginosis (BV) bacteria have on sperm function?

SUMMARY ANSWER: BV toxins dysregulate sperm capacitation and intracellular calcium homeostasis, and impair the ability of sperm to fertilize oocytes.

WHAT IS KNOWN ALREADY: In BV, which is linked to infertility, overgrowth of Prevotella and Gardnerella in the vagina is accompanied by elevated concentrations of the toxins lipopolysaccharide (LPS) and vaginolysin (VLY).

STUDY DESIGN, SIZE, DURATION: This was a laboratory study in which human semen samples were collected from consenting healthy donors with normal semen parameters. Mouse sperm samples were obtained from the caudal …

Rezafungin Versus Caspofungin For The Treatment Of Candidemia And Invasive Candidiasis: Results From The Double-Blind, Randomized, Phase 3 Restore Trial Including The China Extension Study, George R Thompson, Haihui Huang, Sizhou Feng, Yunsong Yu, Alex Soriano, Oliver A Cornely, Bart Jan Kullberg, Peter G Pappas, Marin Kollef, Jose A Vazquez, Patrick M Honore, Laura Cox, Matteo Bassetti

2020-Current year OA Pubs

BACKGROUND: Rezafungin is approved for use in adults with candidemia and/or invasive candidiasis (IC) based on data from the ReSTORE trial (NCT03667690), which demonstrated noninferior efficacy to caspofungin. For regulatory purposes, an additional cohort of patients from China was recruited to ReSTORE. Here, we compared rezafungin and caspofungin in patients with candidemia and/or IC through analysis of the ReSTORE global data plus the China extension study.

METHODS: Adults with candidemia/IC were randomized (1:1) to receive weekly rezafungin (400/200 mg) or daily caspofungin (70/50 mg) for ≤28 days. Noninferiority was concluded for primary efficacy endpoints if the upper bound of the …

Nerve-Associated Macrophages Control Adipose Homeostasis Across Lifespan And Restrain Age-Related Inflammation, Elsie Gonzalez-Hurtado, Maxim N Artyomov, Et Al.

2020-Current year OA Pubs

Age-related inflammation or 'inflammaging' increases disease burden and controls lifespan. Adipose tissue macrophages (ATMs) are critical regulators of inflammaging; however, the mechanisms involved are not well understood in part because the molecular identities of niche-specific ATMs are unknown. Using intravascular labeling to exclude circulating myeloid cells followed by single-cell sequencing with orthogonal validation via multiparametric flow cytometry, we define sex-specific changes and diverse populations of resident ATMs through lifespan in mice. Aging led to depletion of vessel-associated macrophages, expansion of lipid-associated macrophages and emergence of a unique subset of CD38

Muscle Creatine Levels And Sprint Performance In Young Adult Vegans And Vegetarians After 7 Days Of Creatine Monohydrate Supplementation, Thomas Christian Bonne, Valentina Arthemalle, Colin Stephen Doherty, Andreas Breenfeldt Andersen, Jacob Bejder, Thomas Ehlers, Henrik Holm-Sørensen, Nikolai Baastrup Nordsborg

Research outputs 2022 to 2026

Athletes use creatine monohydrate (CM) to enhance high-intensity exercise performance by increasing creatine phosphate availability. While CM supplementation is known to raise muscle creatine levels in vegans and vegetarians, its impact on exercise performance remains uncertain. We examined the effects of CM supplementation on muscle creatine content and exercise performance in vegans and vegetarians. In a randomized, double-blind placebo-controlled design, 15 healthy vegans and vegetarians consumed CM (0.3 g/kg/day, n = 7) or placebo (PLA, 0.3 g maltodextrin/kg/day, n = 8) four times a day for 7 days. Before and after supplementation, repeated sprint capacity was determined. Body mass and …

Antimicrobial Resistance Of Clostridioides (Clostridium) Difficile In Cambodia, Lengsea Eng, Papanin Putsathit, Su Chen Lim, Jessica M. Chisholm, Deirdre A. Collins, Archie C.A. Clements, Kefyalew Addis Alene, Thomas V. Riley

Research outputs 2022 to 2026

Background/Objectives: Antimicrobial resistance (AMR) remains a major topic of interest in infectious disease management. We studied AMR in Clostridioides difficile isolated in Cambodia. Methods: Agar dilution susceptibility testing was performed according to the CLSI guidelines to determine minimal inhibitory concentrations (MICs) of 10 antimicrobials for 192 isolates of C. difficile from four populations in Cambodia: hospitalised adults, hospitalised children, children from an outpatient department (OPD), and healthy adolescents in the community. Results: Using the CLSI MIC breakpoints for anaerobes and EUCAST breakpoints for C. difficile, all isolates were susceptible to vancomycin, metronidazole, fidaxomicin, and amoxicillin/clavulanic acid, and none were resistant …

Comparison Of Decision-Making Skill In 360° Video To The Field Setting In Highly Skilled Rugby Union Players, Hannah J. Vawser, Khaya Morris-Binelli, Benjamin Piggott, Sean Müller, Paola Chivers, Evan Dekker

Research outputs 2022 to 2026

Little evidence exists of whether decision-making in immersive 360° video is representative of the field setting in sport. The purpose of this study was to investigate whether decision-making performance of a representative exemplar competition specific skill was similar between 360° video and a field task. Fifteen players from skilled junior rugby union pathways and professional rugby union teams in Australia were recruited to complete a 360° video decision-making choice response task involving four attackers and three defenders. Their decision-making was previously assessed in a similar field decision-making task that was representative of rugby game play. Decision-making was scored according to …

"I Probably Wouldn't Be As Happy": Childhood Parental Separation And Posttraumatic Growth Narratives From Eight College Students, Meagan C. Arrastia-Chisholm, Hoa Nguyen, Africa Pippin, Heather M. Kelley Ph.D.

Journal of Higher Education & Student Affairs

Parental separation during childhood leads to long-lasting psychological and socioemotional impact on the lives of emerging adults as they transition into higher education. The long-term effects of childhood parental separation on college students' academic performance, physical health, and overall mental wellbeing are well-documented. However, students can develop resilience to cope with challenging family and life experiences and even demonstrate post-traumatic growth. Post-traumatic growth refers to the positive changes and experiences college students discover after enduring life disruptions, such as being separated from their parents at an early age. To better understand the connection between parental separation and posttraumatic growth, a …

S1pr1 Regulates Lymphatic Valve Development And Tertiary Lymphoid Organ Formation In The Ileum, Xin Geng, Gwendalyn J Randolph, Et Al.

2020-Current year OA Pubs

Efficient lymph flow is ensured by lymphatic valves (LVs). The mechanisms that regulate LV development are incompletely understood. Here, we show that the deletion of the GPCR sphingosine 1-phosphate receptor-1 (S1PR1) from lymphatic endothelial cells (LECs) results in fewer LVs. Interestingly, LVs that remained in the terminal ileum-draining lymphatic vessels were specifically dysfunctional. Furthermore, tertiary lymphoid organs (TLOs) formed in the terminal ileum of the mutant mice. TLOs in this location are associated with ileitis in humans and mice. However, mice lacking S1PR1 did not develop obvious characteristics of ileitis. Mechanistically, S1PR1 regulates shear stress signaling and the expression of …

Moderate Coffee And Tea Consumption Is Associated With Slower Cognitive Decline, Stephanie R. Rainey-Smith, Kelsey R. Sewell, Belinda M. Brown, Hamid R. Sohrabi, Ralph N. Martins, Samantha L. Gardener

Research outputs 2022 to 2026

Background: Globally, coffee and tea are consumed extensively, potentially providing neuroprotection through anti-inflammatory and antioxidative stress effects. Objective: This study aimed to investigate associations between coffee and tea intake and cognitive function. Methods: In a longitudinal prospective cohort study, dementia-free (n = 8715; age range 60.0–85.2 years) older adults from the UK Biobank self-reported coffee and tea intake over the previous year; ‘never’, ‘moderate’ (1–3 cups/day), or ‘high’ (≥4 cups/day). Participants completed cognitive assessments at ≥2 timepoints (mean of 9.11 years). Results: Those ‘never’ consuming coffee and ‘moderate’ coffee consumers (β = 0.06, p = 0.005; β = 0.07, p <  0.001, respectively), as well as ‘moderate’ tea consumers and ‘high’ tea consumers (β = 0.06, p = 0.009; β = 0.06, p = 0.003, respectively) had slower fluid intelligence decline. Additionally, those ‘never’ consuming coffee and ‘moderate’ coffee consumers had a slower increase in pairs matching errors (β = −0.05, p = 0.022; β = 0.05, p = 0.013) compared to ‘high’ consumers. Conclusions: ‘Moderate’ coffee, and ‘moderate’ and ‘high’ tea intake may be a protective factor against cognitive decline. Randomized controlled trials are required to establish causal relationships leading to evidence-based recommendations regarding benefits of coffee and tea intake.

The Effect Of Maternal Diet On Neonatal Jaundice: A Case-Control Study, Marzieh Mahdipoor, Alireza J. Motlagh, Hamid S. Baghbanan, Mahmood Bakhtiyari, Zohreh Khodaii, Sara E. Saeieh

Sultan Qaboos University Medical Journal

Objectives: This study aims to investigate the influence of dietary heat intensity in maternal diets on the incidence of neonatal jaundice. Specifically, we examine whether maternal consumption of "dietary heat intensity" foods affects neonatal bilirubin levels, while controlling for potential demographic and obstetric confounding factors. Methods: This retrospective case-control study was conducted between October 2023 and September 2024. The study population consisted of mothers and their newborns attending healthcare centers in Alborz Province, Iran. Based on predefined inclusion and exclusion criteria, 152 newborns were enrolled, comprising 75 non-jaundiced and 77 jaundiced infants. Data were collected using an information-gathering form and …

Cooling Protocols Impact On Nickel-Titanium Rotary Instruments Cyclic Fatigue Resistance Under Extreme Curvatures And Sodium Hypochlorite, Palak Ravjibhai Ranparia, Chintan Joshi, Mona Somani, Aashray Patel, Urooj Desai, Janki Prajapati

Journal of Dentistry Indonesia

Objective: In this work, Hyflex CM, TruNatomy, and Plex V heat-treated nickel titanium rotary instruments were evaluated at body temperature (36 °C ± 1 °C) with and without a cooling protocol to assess the cyclic fatigue resistance. Methods: A 90° curved stainless-steel block was used for cyclic fatigue tests on 60 randomly selected rotary instruments, divided into two groups of 30: body temperature (n = 10) and body temperature with cooling (n = 10). Instruments were further subdivided into 6 groups of 3 different instruments. Cooling protocol group instruments received 5-second spray cooling every 30 seconds, along with continuous 5% …

Auprc: A Metric For Evaluating The Performance Of In-Silico Perturbation Methods In Identifying Differentially Expressed Genes, Hongxu Zhu, Amir Asiaee, Leila Azinfar, Jun Li, Han Liang, Ehsan Irajizad, Kim-Anh Do, James P Long

Faculty, Staff and Student Publications

In silico perturbation models, computational methods that can predict cellular responses to perturbations, present an opportunity to reduce the need for costly and time-intensive in vitro experiments. Many recently proposed models predict high-dimensional cellular responses, such as gene or protein expression to perturbations such as gene knockout or drugs. However, evaluating in silico performance has largely relied on metrics such as $R^{2}$, which assess overall prediction accuracy but fail to capture biologically significant outcomes like the identification of differentially expressed (DE) genes. In this study, we present a novel evaluation framework that introduces the AUPRC metric to assess the precision …

Bacterial Identification And Antibiotic Sensitivity Profiles From Captive Birds At Taipei Zoo, Younjung Chang, I-Li Liu, Jane-Fang Yu, Lih-Chiann Wang

The Thai Journal of Veterinary Medicine

Bacterial infections are common in zoo birds. They may be caused by pathogenic bacteria or opportunistic bacteria due to trauma or stress-induced immune compromise. Improper use of antibiotics contributes to the development of resistant bacteria, making treatment complicated or ineffective. However, comprehensive clinical investigations into bacterial prevalence and antibiotic susceptibility in zoo-captive birds remain limited. This study identified the bacterial species present in captive diseased birds at the zoo and conducted a 12-year analysis of their antibiotic susceptibility profiles. The specimens from 48 birds at Taipei Zoo from 2006 to 2017 were submitted to a commercial reference laboratory for bacterial …

A Qualitative Analysis Of How Just Stop Oil Protests Are Framed In The Uk Online News Media, Holly Handy

The Plymouth Student Scientist

Previous research has found that when the news media reports on demonstrations, protest groups can be subject to delegitimising coverage. This body of research can be subsumed under Chan & Lee’s “protest paradigm” and is believed to consist of a set of narrative frames that journalists default to in the coverage of protests. Recent research demonstrates how the use of these framing devices may be in decline and advocates for a more integrated approach to protest coverage. However, there is little research that uses analytic frameworks specifically theorised to explore media framing. This study uses Giles and Shaw’s media framing …

Deciphering Transcriptomic Changes In Chemobrain: A Comprehensive Review, Tusar Kanta Acharya, Yuan Pan, Peter M Grace, Cobi J Heijnen, Rajasekaran Mahalingam

Faculty, Staff and Student Publications

Chemotherapy-induced cognitive impairments (CICI), colloquially known as "chemobrain," represents a profound and debilitating side effect experienced by a significant number of cancer survivors, impacting their memory, multitasking, and quality of life. This review critically evaluates the molecular mechanisms underlying CICI, with a particular focus on the insights gained from transcriptomic analyses. As cancer incidence rises globally, understanding the complex interplay between chemotherapy agents and their cognitive repercussions becomes increasingly vital. Key mechanisms implicated in CICI include blood-brain barrier disruption, neuroinflammation, and oxidative stress as a result of various chemotherapy treatments, such as doxorubicin, cisplatin, and paclitaxel. We delve into advanced …

Effect Of Garcinol Against Arsenic-Induced Neurobehavioral Alterations And Liver And Kidney Dysfunction In Albino Mice, Abdulmohsen I. Algefare

BioMedicine

The extended exposure to inorganic arsenic is commonly linked to brain damage through oxidative and apoptotic processes. The compound known as Garcinol (GCL) has garnered significant interest due to its potential advantages for human well-being. Nevertheless, the ability of GCL to protect against toxicity generated by arsenic in the brain has not yet been investigated. Hence, our study aimed to examine the neuroprotective effectiveness of GCL in mitigating the detrimental impacts of sodium arsenite (SA) on behavioral patterns, the molecular mechanisms, apoptotic markers, and oxidative stress parameters in the brain, liver, and kidney of mice. The mice were categorized into …

An Investigation Into The Potent Anticancer, Antimicrobial, And Anti-Inflammatory Activities Of A Punica Granatum Nanoemulgel, Ahmad M Eid, Murad Abualhasan, Yara Khaliliya, Zeina Sinan, Aya Khaliliya

BioMedicine

Purpose: The objective of this study is to formulate a nanoemulgel using Punica granatum (P. granatum) seed oil and study the antibacterial, anticancer, and anti-inflammatory properties. Methods: The process to formulate a nanoemulsion from P. granatum seed oil involved using the self-nanoemulsifying technique, with Span 80 and Tween 80 serving as the emulsifying agents. Carbopol hydrogel was combined with the nanoemulsion to produce the nanoemulgel. The particle size, polydispersity index (PDI), rheological behavior, antibacterial, cytotoxic, and anti-inflammatory properties were subsequently examined.

Results: The nanoemulsion formulation with a PDI of 0.229 and a particle size of 189.44 nm was …

Gc-Ms Analysis Of Bioactive Constituents From The Leaves Of Portulaca Afra Jacq. And Portulaca Grandiflora Hook, Sadeq S.K.Al-Taie, Noor F.M. Al-Kenane, Zaidon T. Al-Aqbi

Al-Mustaqbal Journal of Pharmaceutical and Medical Sciences

Portulaca afra and Portulaca grandiflora are traditional medicinal that grow as ornamental plants in Iraq. The present study was conducted for GC-MS analysis of the leaves of these plants to identify possible bioactive compounds. Seventy-nine compounds were recorded in the methanolic extract in Portulaca afra and ninety-two in Portulaca grandiflora. A literature survey revealed that some compounds 4-Vinylphenol, gamma-Sitosterol, Neophytadiene, n-Hexadecanoic acid, Octadecanoic acid, Phytol, Stigmasterol and Tetradecanoic acid possess a variety of biological qualities, including antioxidant, anti-inflammatory, anti-fungal, and anti-cancer effects.

Can Coffee Combat Fatty Liver? A Review Of Emerging Evidence, Mohamed E. Abd El-Hack, Muhammad Arif, Vincenzo Tufarelli, Ayman A. Swelum

Al-Mustaqbal Journal of Pharmaceutical and Medical Sciences

NAFLD (Non-alcoholic fatty liver disease) is an increasingly spread metabolic status characterized by increasing fat accumulation in the liver, obesity closely associated with it, sedentary habits and insulin resistance. Since there is currently no recognized pharmaceutical treatment, lifestyle changes continue to be the mainstay of treatment. Coffee, a globally consumed beverage rich in bioactive and useful compounds like caffeine, cafestol, kahweol, and chlorogenic acids, has emerged as a potential hepatoprotective agent. Evidence from epidemiological experiments, animal studies, and human trials suggests that regular coffee drinking may help decrease hepatic fat levels, improve antioxidant defenses, prevent inflammatory pathways, and prevent or …

In Silico Evaluation Of Nigella Sativa Phytocompounds As Potential Modulators Of Estrogen Receptor Alpha: A Molecular Docking And Admet Study, Murtala Muhammad Jibril, Samuel Otokpa Ede, Ekwere O. Ekwere

Al-Mustaqbal Journal of Pharmaceutical and Medical Sciences

Background: Reproductive dysfunction among women remains a pressing global health issue, often linked to hormonal imbalances involving estrogen receptor alpha (ERα). Nigella sativa has garnered scientific interest due to its diverse phytochemical composition and potential reproductive health benefits. Objective: This study aimed to evaluate selected bioactive compounds from Nigella sativa oil as potential modulators of ERα using advanced computational methods. Methods: A structure-based drug discovery approach was employed. Ten phytocompounds were identified through literature mining and subjected to molecular docking against ERα. Clomiphene citrate, a known selective estrogen receptor modulator (SERM), served as the reference ligand. Binding affinities were analyzed, …

Sevoflurane In Status Asthmaticus: A Case Report And Literature Review, Amelia L. Gurley, Thomas W. Church, Sana Hadyah, Brandon Abrajan, Julia Cheree Glavinic

HCA Healthcare Journal of Medicine

Background

Sevoflurane and other inhaled anesthetics are gradually entering provider awareness as rescue therapies for patients in refractory status asthmaticus. This paper documents a case report and a literature review involving the use of sevoflurane to treat status asthmaticus.

Case Presentation

A 27-year-old man presented to the emergency room in acute hypoxic respiratory failure due to status asthmaticus, refractory to multiple therapies and requiring intubation. He was successfully extubated following sevoflurane administration in the operating room, and his care was complicated by transient hypotension. A multidisciplinary effort was critical in ensuring successful and timely therapy.

Methods

We performed a literature …

Dynamics And Malleability Of Plant Dna Methylation During Abiotic Stresses, Niraj Lodhi, Rakesh Srivastava

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Epigenetic regulation, particularly DNA methylation, plays a crucial role in plant adaptation to environmental stresses by modulating gene expression without altering the underlying DNA sequence. In response to major abiotic stresses such as salinity, drought, heat, cold, and heavy metal toxicity, plants undergo dynamic changes in DNA methylation patterns. These modifications are orchestrated by DNA methyltransferases and demethylases with variations depending on plant species, genetic background, and ontogenic phase. DNA methylation affects the expression of key genes involved in cellular, physiological, and metabolic processes essential for stress tolerance. Furthermore, it contributes to the establishment of stress memory, which can be …

Ras-Membrane Interaction And Oligomerization: There Is More Than Meets The Eye, Abraham C Sianoya, Vijay K Bhardwaj, Alemayehu A Gorfe

Faculty, Staff and Student Publications

Membrane association is fundamental to Rat sarcoma (RAS) function, driving both its physiologic signaling and oncogenic transformation. This review consolidates recent advances in the study of RAS-membrane interactions, emphasizing the molecular mechanisms underlying its membrane engagement and oligomerization. We first discuss the roles of RAS lipid modification and conformational diversity of its intrinsically disordered C-terminus in these processes, and we then examine the debate surrounding RAS dimerization and its potential role in the formation of higher-order oligomers. By integrating emerging insights into these issues, we offer our own perspectives on the driving forces of RAS oligomerization and propose potential new …

Investigating “Sled Head” Phenomenon And Diurnal Patterns In Team Usa Bobsleigh And Luge Athletes: A Virtual Research Study, Garrett R. Augsburger

Doctoral Dissertations and Projects

This study investigated the relationship between training time, performance, and concussion symptoms among Team USA bobsleigh and luge athletes during a four-week training season. Recognizing the high physical demands and potential risks associated with sledding sports, the study aimed to understand the prevalence of concussion-related experiences and how training schedules might influence athletic performance and symptomology. Repeated measures quantitative design was employed using session-level survey data submitted by athletes following daily training sessions. Data was collected through a structured questionnaire assessing variables such as performance rating, fatigue, alertness, training intensity, sleep quality, and concussion symptoms. A total of 156 sessions …

Opiate Addiction Crisis In The United States, Autumn Joy Collins

Doctoral Dissertations and Projects

This research investigates how fentanyl has influenced drug trafficking trends and the corresponding response from law enforcement in New York State. It aims to explore the changes in drug trafficking pathways, organizational structures, and methods in this region since fentanyl's emergence and how state law enforcement has adjusted to this new challenge. The study uses a quantitative methodology, analyzing various data from law enforcement records, court documents, and state government reports. The focus is on fentanyl-related drug trafficking cases in New York State over five years. Methods include examining case records and analyzing trafficking patterns statistically. The study will assess …

Has Regional Hospital Autonomy Achieved Its Goals? Lessons Learned From Indonesia: A Systematic Review, Masyitoh Basabih, Indriaswari Widhakuswara

Kesmas

This study examined the impact of hospital autonomy implementation on the performance of regional public hospitals in Indonesia, particularly in terms of efficiency, effectiveness, and governance. A systematic review of 32 peer-reviewed studies published between June 12 and September 6, 2023, was conducted using Garba Rujukan Digital and Google Scholar, following PRISMA guidelines. Studies addressing financial performance, service outcomes, and challenges related to implementing autonomy were included. The findings indicated that while autonomy led to improvements in infrastructure, service types, visit volumes, and hospital revenues, it had only a marginal impact on improving key health performance outcomes, such as service …

Excitotoxic Neuronal Death Requires Superoxide Entry Into Neurons Through Volume-Regulated Anion Channels, Kate Harris, Seok Joon Won, Gokhan Uruk, Nguyen Mai, Devran Ogut, Yonghui Zhao, Litao Xie, Paul Baxter, Katharina Everaerts, Rajan Sah, Raymond A Swanson

2020-Current year OA Pubs

Neuronal death in stroke and other conditions stems in part from stimulation of

Prefoldin Complex Promotes Interferon-Stimulated Gene Expression And Is Inhibited By Rotavirus Vp3, Yinxing Zhu, Yanhua Song, Dilip Kumar, Peter K Jackson, B V Venkataram Prasad, Siyuan Ding

2020-Current year OA Pubs

Timely induction of interferons and interferon-stimulated genes (ISGs) is critical for successful host defense against viral infections. VP3 encoded by rotavirus is implicated in interferon antagonism. However, the precise mechanisms remain incompletely understood. By conducting tandem-affinity purification coupled with high-resolution mass spectrometry, we identify the prefoldin complex as the top cellular binding partner of VP3. Rotavirus infection is significantly enhanced in prefoldin subunit knockout cells. Using proteome-wide label-free quantification, we find that prefoldin assists in folding ubiquitin-like-modifier-activating-enzyme-3 (UBA3), both of which positively regulate ISG expression. Through direct and competitive binding, VP3 interferes with the chaperone activity of prefoldin, leading to …