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Association Analysis Of Copy Numbers Of Fc-Gamma Receptor Genes For Rheumatoid Arthritis And Other Immune-Mediated Phenotypes, L. Franke, H. El Bannoudi, D. T. Jansen, K. Kok, G. Trynka, D. Diogo, M. Swertz, K. Fransen, P. K. Gregersen, A. Zhernakova, +16 Additional Authors Jan 2016

Association Analysis Of Copy Numbers Of Fc-Gamma Receptor Genes For Rheumatoid Arthritis And Other Immune-Mediated Phenotypes, L. Franke, H. El Bannoudi, D. T. Jansen, K. Kok, G. Trynka, D. Diogo, M. Swertz, K. Fransen, P. K. Gregersen, A. Zhernakova, +16 Additional Authors

Journal Articles

Segmental duplications (SDs) comprise about 5% of the human genome and are enriched for immune genes. SD loci often show copy numbers variations (CNV), which are difficult to tag with genotyping methods. CNV in the Fcgamma receptor region (FCGR) has been suggested to be associated with rheumatic diseases. The objective of this study was to delineate association of FCGR-CNV with rheumatoid arthritis (RA), coeliac disease and Inflammatory bowel disease incidence. We developed a method to accurately quantify CNV in SD loci based on the intensity values from the Immunochip platform and applied it to the FCGR locus. We determined the …

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Dense Genotyping Of Immune-Related Loci In Idiopathic Inflammatory Myopathies Confirms Hla Alleles As The Strongest Genetic Risk Factor And Suggests Different Genetic Background For Major Clinical Subgroups, S. Rothwell, R. G. Cooper, I. E. Lundberg, F. W. Miller, P. K. Gregersen, J. Bowes, J. Vencovsky, K. Danko, V. Limaye, J. A. Lamb, +26 Additional Authors Jan 2016

Dense Genotyping Of Immune-Related Loci In Idiopathic Inflammatory Myopathies Confirms Hla Alleles As The Strongest Genetic Risk Factor And Suggests Different Genetic Background For Major Clinical Subgroups, S. Rothwell, R. G. Cooper, I. E. Lundberg, F. W. Miller, P. K. Gregersen, J. Bowes, J. Vencovsky, K. Danko, V. Limaye, J. A. Lamb, +26 Additional Authors

Journal Articles

OBJECTIVES: The idiopathic inflammatory myopathies (IIMs) are a heterogeneous group of rare autoimmune diseases characterised by muscle weakness and extramuscular manifestations such as skin rashes and interstitial lung disease. We genotyped 2566 IIM cases of Caucasian descent using the Immunochip; a custom array covering 186 established autoimmune susceptibility loci. The cohort was predominantly comprised of patients with dermatomyositis (DM, n=879), juvenile DM (JDM, n=481), polymyositis (PM, n=931) and inclusion body myositis (n=252) collected from 14 countries through the Myositis Genetics Consortium. RESULTS: The human leucocyte antigen (HLA) and PTPN22 regions reached genome-wide significance (p

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Loci Associated With N-Glycosylation Of Human Igg Are Not Associated With Rheumatoid Arthritis: A Mendelian Randomisation Study, A. Yarwood, S. Viatte, Y. Okada, R. Plenge, K. Yamamoto, A. Barton, D. Symmons, S. Raychaudhuri, P. Gregersen, S. Eyre, +2 Additional Authors Jan 2016

Loci Associated With N-Glycosylation Of Human Igg Are Not Associated With Rheumatoid Arthritis: A Mendelian Randomisation Study, A. Yarwood, S. Viatte, Y. Okada, R. Plenge, K. Yamamoto, A. Barton, D. Symmons, S. Raychaudhuri, P. Gregersen, S. Eyre, +2 Additional Authors

Journal Articles

OBJECTIVES: A recent study identified 16 genetic variants associated with N-glycosylation of human IgG. Several of the genomic regions where these single nucleotide polymorphisms (SNPs) reside have also been associated with autoimmune disease (AID) susceptibility, suggesting there may be pleiotropy (genetic sharing) between loci controlling both N-glycosylation and AIDs. We investigated this by testing variants associated with levels of IgG N-glycosylation for association with rheumatoid arthritis (RA) susceptibility using a Mendelian randomisation study, and testing a subset of these variants in a less well-powered study of treatment response and severity. METHODS: SNPs showing association with IgG N-glycosylation were analysed for …

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Lower Omega-3 Fatty Acids Are Associated With The Presence Of Anti-Cyclic Citrullinated Peptide Autoantibodies In A Population At Risk For Future Rheumatoid Arthritis: A Nested Case-Control Study, R. W. Gan, K. A. Young, G. O. Zerbe, M. K. Demoruelle, M. H. Weisman, J. H. Buckner, P. K. Gregersen, T. R. Mikuls, J. R. O'Dell, J. M. Norris, +4 Additional Authors Jan 2016

Lower Omega-3 Fatty Acids Are Associated With The Presence Of Anti-Cyclic Citrullinated Peptide Autoantibodies In A Population At Risk For Future Rheumatoid Arthritis: A Nested Case-Control Study, R. W. Gan, K. A. Young, G. O. Zerbe, M. K. Demoruelle, M. H. Weisman, J. H. Buckner, P. K. Gregersen, T. R. Mikuls, J. R. O'Dell, J. M. Norris, +4 Additional Authors

Journal Articles

OBJECTIVE: The aim of this study was to investigate omega-3 fatty acid (FA) supplement use and omega-3 FAs in erythrocyte membranes [omega-3 FA % in erythrocyte membranes (RBC)] and their association with anti-CCP autoantibodies in a population without RA, but who are at genetic risk for RA. METHODS: The multicentre Studies of the Etiology of RA (SERA) cohort includes RA-free subjects who are first-degree relatives of RA probands or are enriched with the HLA-DR4 allele. In a nested case-control study, 30 SERA cases were identified who were anti-CCP2 antibody positive. We further identified 47 autoantibody negative controls, frequency matched to …

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What Is Damaging The Kidney In Lupus Nephritis?, A. Davidson Jan 2016

What Is Damaging The Kidney In Lupus Nephritis?, A. Davidson

Journal Articles

Despite marked improvements in the survival of patients with severe lupus nephritis over the past 50 years, the rate of complete clinical remission after immune suppression therapy is

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Whole-Exome Sequencing In Relapsing Chronic Lymphocytic Leukemia: Clinical Impact Of Recurrent Rps15 Mutations, V. Ljungstrom, D. Cortese, E. Young, T. Pandzic, L. Mansouri, K. Plevova, S. Ntoufa, P. Baliakas, N. Chiorazzi, R. Rosenquist, +24 Additional Authors Jan 2016

Whole-Exome Sequencing In Relapsing Chronic Lymphocytic Leukemia: Clinical Impact Of Recurrent Rps15 Mutations, V. Ljungstrom, D. Cortese, E. Young, T. Pandzic, L. Mansouri, K. Plevova, S. Ntoufa, P. Baliakas, N. Chiorazzi, R. Rosenquist, +24 Additional Authors

Journal Articles

Fludarabine, cyclophosphamide and rituximab (FCR) is first-line treatment for medically fit chronic lymphocytic leukemia (CLL) patients, however despite good response rates many patients eventually relapse. Whilst recent high-throughput studies have identified novel recurrent genetic lesions in adverse-prognostic CLL, the mechanisms leading to relapse after FCR therapy are not completely understood. To gain insight into this issue, we performed whole-exome sequencing of sequential samples from 41 CLL patients who were uniformly treated with FCR but relapsed after a median of 2 years. In addition to mutations with known adverse-prognostic impact (TP53, NOTCH1, ATM, SF3B1, NFKBIE, BIRC3) a large proportion of cases …

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D-Serine Is A Substrate For Neutral Amino Acid Transporters Asct1/Slc1a4 And Asct2/Slc1a5, And Is Transported By Both Subtypes In Rat Hippocampal Astrocyte Cultures, Alan C. Foster, Jill Farnsworth, Genevieve Lind, Yong-Xin Lin, Jia-Ying Yang, Van Dang, Mahmud Penjwini, Veena Viswanath, Ursula Staubli, Michael Kavanaugh Jan 2016

D-Serine Is A Substrate For Neutral Amino Acid Transporters Asct1/Slc1a4 And Asct2/Slc1a5, And Is Transported By Both Subtypes In Rat Hippocampal Astrocyte Cultures, Alan C. Foster, Jill Farnsworth, Genevieve Lind, Yong-Xin Lin, Jia-Ying Yang, Van Dang, Mahmud Penjwini, Veena Viswanath, Ursula Staubli, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

N-methyl-D-aspartate (NMDA) receptors play critical roles in synaptic transmission and plasticity. Activation of NMDA receptors by synaptically released L-glutamate also requires occupancy of co-agonist binding sites in the tetrameric receptor by either glycine or D-serine. Although D-serine appears to be the predominant co-agonist at synaptic NMDA receptors, the transport mechanisms involved in D-serine homeostasis in brain are poorly understood. In this work we show that the SLC1 amino acid transporter family members SLC1A4 (ASCT1) and SLC1A5 (ASCT2) mediate homo- and hetero-exchange of D-serine with physiologically relevant kinetic parameters. In addition, the selectivity profile of D-serine uptake in cultured rat hippocampal …

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Sensitization To Food And Inhalant Allergens In Healthy Children In Van, East Turkey, Mehmet Parlak, Ayteki̇n Çikman, Yasemi̇n Bayram, Nesri̇n Ceylan, Ulaş Çalişir, Mustafa Berktaş Jan 2016

Sensitization To Food And Inhalant Allergens In Healthy Children In Van, East Turkey, Mehmet Parlak, Ayteki̇n Çikman, Yasemi̇n Bayram, Nesri̇n Ceylan, Ulaş Çalişir, Mustafa Berktaş

Turkish Journal of Medical Sciences

Background/aim: The purpose of this study was to determine the most frequent food and inhalant allergens leading to allergic sensitization in children in Van Province of Turkey. Materials and methods: The study included 1052 serum samples with no diagnosis of allergy. The sera were tested with the Euroline Pediatric IgE test kit (EUROIMMUN, Germany). By using the EUROLineScan digital evaluation system, the intensity of bands was calculated with enzyme allergosorbent test classification. Results: Out of the 1052 tested sera, 143 were found to be cross-reactive carbohydrate determinant-positive and were discarded from the study. Of the remaining 909 sera, 513 (56%) …

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Comparison Of Galactomannan, Beta-D-Glucan, And Aspergillus Dna In Sera Of High-Risk Adult Patients With Hematological Malignancies For The Diagnosis Of Invasive Aspergillosis, Gülçi̇n Bölük, Esra Kazak, Fahi̇r Özkalemkaş, Beyza Ener, Hali̇s Akalin, Harun Ağca, Yildiz Okuturlar, Kürşad Keski̇n, Başak Burgazlioğlu, Ridvan Ali̇ Jan 2016

Comparison Of Galactomannan, Beta-D-Glucan, And Aspergillus Dna In Sera Of High-Risk Adult Patients With Hematological Malignancies For The Diagnosis Of Invasive Aspergillosis, Gülçi̇n Bölük, Esra Kazak, Fahi̇r Özkalemkaş, Beyza Ener, Hali̇s Akalin, Harun Ağca, Yildiz Okuturlar, Kürşad Keski̇n, Başak Burgazlioğlu, Ridvan Ali̇

Turkish Journal of Medical Sciences

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Fully Automated Morphological Analysis Of Patients With Obstructive Sleep Apnea, Oktay Alğin, Burak Akin, Gökhan Ocakoğlu, Evri̇m Özmen Jan 2016

Fully Automated Morphological Analysis Of Patients With Obstructive Sleep Apnea, Oktay Alğin, Burak Akin, Gökhan Ocakoğlu, Evri̇m Özmen

Turkish Journal of Medical Sciences

Background/aim: Obstructive sleep apnea syndrome (OSAS) is a disease characterized by episodic hypoxia. We aimed to use the Freesurfer program for global evaluation of morphological changes in OSAS patients. Materials and methods: Three-dimensional T1-weighted images were obtained, and intracranial morphology was assessed in 18 patients with OSAS and 20 controls. Results of the volume and the cortical thickness analyses of both groups were compared statistically. Results: The total cortical, left-right hemispheres gray matter (GM), corpus callosum, and total GM volumes were lower in OSAS patients when compared with the control group (P < 0.001). The average cortical thickness was lower in OSAS patients bilaterally in pars orbitalis, paracentral, rostral middle frontal, middle frontal, orbital, and superior frontal gyri when compared with the control group (P < 0.05). Furthermore, the volume and average cortical thickness of multiple anatomic regions, apart from the brain parts mentioned above, were decreased unilaterally (e.g., hippocampus, cingulum, putamen, thalamus) in OSAS patients (P < 0.05). Conclusion: Multiple morphologic changes occur in the cerebral structures of OSAS patients due to intermittent ischemia episodes. Detection of those areas with Freesurfer is easier. New studies with large series would be needed for these subjects.

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The Role Of Fdg Pet/Ct In Detection Of Distant Metastasis In Theinitial Staging Of Breast Cancer, Anar Aliyev, Sabi̇re Yilmaz Aksoy, Meftune Özhan, Özgül Ekmekçi̇oğlu, Betül Vatankulu, Pinar Çi̇ğdem Kocael, Muhammet Sai̇t Sağer, Meli̇h Engi̇n Erkan, Keri̇m Sönmezoğlu, Meti̇n Halaç Jan 2016

The Role Of Fdg Pet/Ct In Detection Of Distant Metastasis In Theinitial Staging Of Breast Cancer, Anar Aliyev, Sabi̇re Yilmaz Aksoy, Meftune Özhan, Özgül Ekmekçi̇oğlu, Betül Vatankulu, Pinar Çi̇ğdem Kocael, Muhammet Sai̇t Sağer, Meli̇h Engi̇n Erkan, Keri̇m Sönmezoğlu, Meti̇n Halaç

Turkish Journal of Medical Sciences

Background/aim: We aimed to evaluate the role of FDG PET/CT in the detection of extraaxillary regional nodal/distant metastasis in breast cancer patients and to assess the value of FDG PET/CT for detecting distant metastases in patient subgroups. Materials and methods: A total of 254 patients with breast cancer (248 female, 6 male) who underwent PET/CT for initial staging were enrolled. Patients were divided into four groups: Group 1 consisted of 154 patients diagnosed by tru-cut/core/FNAB, Group 2 comprised 32 patients diagnosed by excisional biopsy, Group 3 included 62 patients who had mastectomy-axillary lymph node dissection, and Group 4 consisted of …

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Distribution Of Ldl Subgroups In Patients With Hyperlipidemia, Ebru Taylan, Emi̇ne Pinar Tuncel Jan 2016

Distribution Of Ldl Subgroups In Patients With Hyperlipidemia, Ebru Taylan, Emi̇ne Pinar Tuncel

Turkish Journal of Medical Sciences

Background/aim: Low-density lipoproteins (LDLs) have been shown to be a major risk factor for coronary artery disease. Multiple distinct subspecies have been identified among LDL particles on the basis of differences in size, density, and chemical composition. Particles with a diameter of

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Evaluation And Identification Of Idua Gene Mutations In Turkishpatients With Mucopolysaccharidosis Type I, Nazente Atçeken, Riza Köksal Özgül, Di̇dem Yücel Yilmaz, Ayşegül Tokatli, Turgay Coşkun, Hati̇ce Serap Si̇vri̇, Ali̇ Dursun, Mehmet Karaca Jan 2016

Evaluation And Identification Of Idua Gene Mutations In Turkishpatients With Mucopolysaccharidosis Type I, Nazente Atçeken, Riza Köksal Özgül, Di̇dem Yücel Yilmaz, Ayşegül Tokatli, Turgay Coşkun, Hati̇ce Serap Si̇vri̇, Ali̇ Dursun, Mehmet Karaca

Turkish Journal of Medical Sciences

Background/aim: This study aimed to identify IDUA gene mutations in Turkish patients morphologically (phenotypic) diagnosed with MPS type I. It also sought to discuss the possible effects of detected mutations on alpha-L-iduronidase enzyme function based on current knowledge. Materials and methods: Genetic analysis was carried out in 15 patients using direct DNA sequencing. Moreover, segregation analysis was performed among family members to predict the pathogenic effect of novel mutations, and computational programs were used to predict their functional impact. Results: Nine different mutations (c.494-1G>A, c.793-6C>G, c.793-5C>A, p.M1L, p.Y64X, p.A327P, p.W402X, p.P533L, and p.R628X) were identified. Computational analysis …

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The Role Of Baseline Ga-68 Dotatate Positron Emission Tomography/Computed Tomography In The Prediction Of Response To Fixed-Dose Peptide Receptor Radionuclide Therapy With Lu-177 Dotatate*, Çi̇ğdem Soydal, Ahmet Peker, Elgi̇n Özkan, Nuri̇ye Özlem Küçük, Kemal Meti̇n Kir Jan 2016

The Role Of Baseline Ga-68 Dotatate Positron Emission Tomography/Computed Tomography In The Prediction Of Response To Fixed-Dose Peptide Receptor Radionuclide Therapy With Lu-177 Dotatate*, Çi̇ğdem Soydal, Ahmet Peker, Elgi̇n Özkan, Nuri̇ye Özlem Küçük, Kemal Meti̇n Kir

Turkish Journal of Medical Sciences

Background/aim: To describe the role of baseline gallium (Ga)-68 DOTATATE positron emission tomography (PET)/computed tomography (CT) in the prediction of the response to peptide receptor radionuclide therapy (PRRT) using lutetium (Lu)-177 DOTATATE. Materials and methods: Analysis was made of baseline Ga-68 DOTATATE PET/CT images of 29 patients (17 females and 12 males; mean age: 50.7 ± 14.6 years) with metastatic neuroendocrine tumors who received PRRT with Lu-177 DOTATATE. Maximum standardized uptake values (SUVmax) of reference lesions and their ratios to physiological uptake organs were calculated. The relationship between these values and the radiological response was analyzed. Results: Partial response was …

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Amniotic Membrane Transplantation In Bacterial And Herpetic Stromal Keratitis, Yeşi̇m Altay, Sema Tamer, Ayşe Burcu, Özgür Balta Jan 2016

Amniotic Membrane Transplantation In Bacterial And Herpetic Stromal Keratitis, Yeşi̇m Altay, Sema Tamer, Ayşe Burcu, Özgür Balta

Turkish Journal of Medical Sciences

Background/aim: The aim of this study was to describe the results of amniotic membrane transplantation (AMT) in patients with bacterial and herpetic stromal keratitis. Materials and methods: This was a retrospective chart review study including 42 patients with herpetic keratitis (group 1) and 42 patients with bacterial keratitis (group 2). AMT was performed in addition to antimicrobial therapy. Topical steroids were administered after surgery. The outcome parameters evaluated were epithelialization time, decrease of stromal inflammation, and uncorrected visual acuity (UCVA). Results: The average age of our patients was 55.85 ± 19.07 years, and average follow-up was 14.70 ± 11.75 months. …

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Glycemic Index Values Of Monofloral Turkish Honeys And The Effect Oftheir Consumption On Glucose Metabolism*, Ali̇ Ti̇muçi̇n Atayoğlu, Meltem Soylu, Si̇bel Si̇li̇ci̇, Neri̇man İnanç Jan 2016

Glycemic Index Values Of Monofloral Turkish Honeys And The Effect Oftheir Consumption On Glucose Metabolism*, Ali̇ Ti̇muçi̇n Atayoğlu, Meltem Soylu, Si̇bel Si̇li̇ci̇, Neri̇man İnanç

Turkish Journal of Medical Sciences

Background/aim: Clinical trials have shown that low glycemic index (GI) nutrition reduces mean blood glucose concentrations and insulin secretions. The aim of the present study was to determine the GI values of various monofloral (citrus, milk-vetch, chestnut, thyme, lime, pine) honeys of Turkey, and the effect of their consumption on glucose metabolism. Materials and methods: Processing data from 20 healthy volunteers, GI values were determined from the glycemia values by using the incremental area method. Serum insulin and C-peptide levels were also measured before and 120 min after the test. Results: The GI values of citrus, thyme, lime, chestnut, pine, …

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Evaluation Of Maternal Serum Folate, Vitamin B12, And Homocysteine Levels Andfactor V Leiden, Factor Ii G.20210g>A, And Mthfr Variations In Prenatallydiagnosed Neural Tube Defects, Hati̇p Aydin, Resul Arisoy, Ali̇ Karaman, Emre Erdoğdu, Arda Çeti̇nkaya, Bi̇lgen Bi̇lge Geçki̇nli̇, Hasan Şi̇mşek, Oya Demi̇rci̇ Jan 2016

Evaluation Of Maternal Serum Folate, Vitamin B12, And Homocysteine Levels Andfactor V Leiden, Factor Ii G.20210g>A, And Mthfr Variations In Prenatallydiagnosed Neural Tube Defects, Hati̇p Aydin, Resul Arisoy, Ali̇ Karaman, Emre Erdoğdu, Arda Çeti̇nkaya, Bi̇lgen Bi̇lge Geçki̇nli̇, Hasan Şi̇mşek, Oya Demi̇rci̇

Turkish Journal of Medical Sciences

Background/aim: Neural tube defects (NTDs) are common congenital malformations that develop as a result of interactions between several genes and environmental factors. Many factors have been investigated in order to understand the etiology of NTDs, and many studies have identified folate intake as a common contributing factor. The exact etiology of the disease is still unknown. Materials and methods: In this study, we compared serum folate, vitamin B12, and homocysteine levels, along with common thrombophilia-related genetic variations, including factor V Leiden, factor II g.20210G>A, MTHFR c.677C>T, and MTHFR c.1298A>C, in 35 pregnant women with fetal NTDs and …

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Free Radical-Induced Nephrotoxicity Following Repeated Oral Exposureto Chlorpyrifos Alone And In Conjunction With Fluoride In Rats, Naseer Baba, Rajinder Raina, Pawan Verma, Mudasir Sultana Jan 2016

Free Radical-Induced Nephrotoxicity Following Repeated Oral Exposureto Chlorpyrifos Alone And In Conjunction With Fluoride In Rats, Naseer Baba, Rajinder Raina, Pawan Verma, Mudasir Sultana

Turkish Journal of Medical Sciences

Background/aim: Chronic renal disorder is becoming a major health problem worldwide. The purpose of the present study was to investigate alterations in the renal antioxidant system in rats induced by repeated exposure to chlorpyrifos (CPF) alone and in conjunction with fluoride. Materials and methods: Wistar rats were randomly allocated to seven groups, each consisting of six rats, and were subjected to different treatment regiments for 28 days. Results: Significant increases (P < 0.05) in plasma protein, blood urea nitrogen, and creatinine levels indicated alterations in renal functions on repeated exposure to CPF or fluoride; moreover, these changes were more pronounced in animals exposed to both toxicants concurrently. A significant increase (P < 0.05) in malondialdehyde levels and decreases in superoxide dismutase, catalase, and glutathione peroxidase activities in renal tissue were noted, indicating renal damage on exposure to CPF, fluoride, or the combination of those. Conclusion: Our observations suggested that the concurrent exposure to CPF and fluoride increased the extent of renal damage. These findings indicate that this damage is due to increased free radical formation and a reduced function of the antioxidant system in renal tissue. Thus, the application of CPF as an insecticide should be reduced in areas where the fluoride levels in ground waters are high in order to minimize renal damage in exposed populations.

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Comparison Between The Protective Effects Of Vitamin K And Vitamin A On The Modulation Of Hypervitaminosis D3 Short-Term Toxicity In Adult Albino Rats, Said Said Elshama, Hosam-Eldin Hussein Osman, Ayman El-Meghawry El-Kenawy, Hamdi Mohamed Youseef Jan 2016

Comparison Between The Protective Effects Of Vitamin K And Vitamin A On The Modulation Of Hypervitaminosis D3 Short-Term Toxicity In Adult Albino Rats, Said Said Elshama, Hosam-Eldin Hussein Osman, Ayman El-Meghawry El-Kenawy, Hamdi Mohamed Youseef

Turkish Journal of Medical Sciences

Background/aim: Vitamin D3 has increased risk of toxicity due to its common use in multivitamin preparations. Vitamin K and vitamin A play an important role in vitamin D action. The goal of the current study was to compare the protective effects of vitamin K and vitamin A on the modulation of hypervitaminosis D3 toxicity in rats by assessing serum calcium, renal function tests, cardiac enzymes, and related histopathological changes. Materials and methods: Eighty adult albino rats were divided into four groups; each group consisted of 20 rats. The first group received water; the second received a toxic dose of vitamin …

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Evaluation Of The Effectiveness Of Biodegradable Electrospun Caprolactoneand Poly(Lactic Acid-?-Caprolactone) Nerve Conduits For Peripheral Nerveregenerations In A Rat Sciatic Nerve Defect Model, Mehmet Dadaci, Ni̇met Karagülle, Erhan Sönmez, Zeynep Dadaci, Evren Tevfi̇k İşci̇, Bi̇lsev İnce, İbrahi̇m Vargel, Erhan Pi̇şki̇n, Ali̇ Yücel Erk Jan 2016

Evaluation Of The Effectiveness Of Biodegradable Electrospun Caprolactoneand Poly(Lactic Acid-?-Caprolactone) Nerve Conduits For Peripheral Nerveregenerations In A Rat Sciatic Nerve Defect Model, Mehmet Dadaci, Ni̇met Karagülle, Erhan Sönmez, Zeynep Dadaci, Evren Tevfi̇k İşci̇, Bi̇lsev İnce, İbrahi̇m Vargel, Erhan Pi̇şki̇n, Ali̇ Yücel Erk

Turkish Journal of Medical Sciences

Background/aim: The aim of this study was to compare electrospun caprolactone (EC) and poly(lactic acid-?-caprolactone) (PLCL) nerve conduits with nerve graft in a rat sciatic nerve defect model. Materials and methods: A total of 32 male Wistar albino rats were divided into 4 groups, with 8 rats in each group. A nerve defect of 1 cm was constructed in the left sciatic nerve of the rats. These defects were left denuded in the sham group, and reconstructed with nerve grafts, PLCL, and EC nerve conduits in the other groups. After 3 months, nerve regenerations were evaluated macroscopically, microscopically, and electrophysiologically. …

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Ameliorating Effects Of Curcumin On Nicotine-Induced Mice Testes, Gülfi̇dan Coşkun, Hülya Özgür, Şaban Doran, Sai̇t Polat Jan 2016

Ameliorating Effects Of Curcumin On Nicotine-Induced Mice Testes, Gülfi̇dan Coşkun, Hülya Özgür, Şaban Doran, Sai̇t Polat

Turkish Journal of Medical Sciences

Background/aim: The aim of this study was to determine the antioxidative effect of curcumin on nicotine-induced mice testis. Materials and methods: Sixty Swiss albino male mice were divided into five groups, each containing 12 mice. The first group was used as a control. To induce toxicity in the second and third group, nicotine (0.4 mg/kg/day) was injected intraperitoneally into mice for 14 and 28 days, respectively. The mice in the fourth and fifth group were injected with nicotine (0.4 mg/kg/day) and orally treated with curcumin (200 mg/kg) for 14 and 28 days, respectively. Testosterone levels were measured from blood samples …

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Role Of Flt3 In The Proliferation And Aggressiveness Of Hepatocellular Carcinoma, Muammer Merve Aydin, Nermi̇n Sumru Bayin, Tolga Acun, Mustafa Cengi̇z Yakicier, Kami̇l Can Akçali Jan 2016

Role Of Flt3 In The Proliferation And Aggressiveness Of Hepatocellular Carcinoma, Muammer Merve Aydin, Nermi̇n Sumru Bayin, Tolga Acun, Mustafa Cengi̇z Yakicier, Kami̇l Can Akçali

Turkish Journal of Medical Sciences

Background/aim: Previously we showed that Fms-like tyrosine kinase (FLT3) changes its cellular localization upon partial hepatectomy, suggesting a role in liver regeneration. FLT3 was also shown to play an important function in cellular proliferation and activation of PI3K and Ras. Thus, we aimed to investigate the role of FLT3 in hepatocellular tumorigenesis utilizing in vitro and in vivo models. Materials and methods: We used Snu398 cells that express FLT3. We investigated these cells' in vitro proliferation and invasion abilities by treatment with the FLT3 inhibitor K-252a or by knocking-down with FLT3 shRNA,. Furthermore, the effect of blocking FLT3 activity and …

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Correlation Between Hfe Gene Polymorphisms And Increased Risk Of Coronary Artery Disease Among Patients With Type 2 Diabetes In Iran, Leila Saremi, Marzieh Saremi, Shirin Lotfipanah, Saber Imani, Junjiang Fu, Tianyu Zhang Jan 2016

Correlation Between Hfe Gene Polymorphisms And Increased Risk Of Coronary Artery Disease Among Patients With Type 2 Diabetes In Iran, Leila Saremi, Marzieh Saremi, Shirin Lotfipanah, Saber Imani, Junjiang Fu, Tianyu Zhang

Turkish Journal of Medical Sciences

Background/aim: Diabetes mellitus is a risk factor for cardiovascular diseases (CVDs), which are among the major causes of deaths in type 2 diabetes (T2D). The purpose of the present study was to determine the association of C282Y and H63D mutations in the HFE gene with increased risk of coronary artery disease (CAD) in T2D patients. Materials and methods: Two hundred and ninety individuals were divided into two groups: a case group and a control group. Genomic DNA of peripheral venous blood cells was extracted and the HFE gene mutations were analyzed using the PCR-RFLP technique. Results: Data analysis revealed a …

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The Antioxidant And Antimutagenic Activities Of Ankaferd Blood Stopper,A Natural Hemostatic Agent Used In Dentistry, Aysel Uğur, Nurdan Saraç, Di̇lek Aynur Çankal, Murat Özle Jan 2016

The Antioxidant And Antimutagenic Activities Of Ankaferd Blood Stopper,A Natural Hemostatic Agent Used In Dentistry, Aysel Uğur, Nurdan Saraç, Di̇lek Aynur Çankal, Murat Özle

Turkish Journal of Medical Sciences

Background/aim: This study investigated the antioxidant and antimutagenic properties of Ankaferd blood stopper (ABS), a plant-based topical hemostatic agent used in Turkey to treat external hemorrhages and bleeding during dental surgery. While previous studies have examined the antimicrobial, antiinflammatory, and anticarcinogenic properties of ABS, to our knowledge, this is the first study to report on the antioxidant and antimutagenic activities of this drug. Materials and methods: Antioxidant activity was evaluated using DPPH radical-scavenging and β-carotene-linoleic acid tests. Antimutagenic activity was assessed using the Ames Salmonella/microsome mutagenicity test with the bacterial mutant strains Salmonella typhimurium TA98 and TA100. Results: Although ABS …

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Enos Gene Polymorphisms In Paraffin-Embedded Tissues Of Prostate Cancer Patients, Fi̇kri̇ye Polat, Nesri̇n Turaçlar, Meral Yilmaz, Günsel Bi̇ngöl, Hasi̇be Ci̇ngi̇lli̇ Vural Jan 2016

Enos Gene Polymorphisms In Paraffin-Embedded Tissues Of Prostate Cancer Patients, Fi̇kri̇ye Polat, Nesri̇n Turaçlar, Meral Yilmaz, Günsel Bi̇ngöl, Hasi̇be Ci̇ngi̇lli̇ Vural

Turkish Journal of Medical Sciences

Background/aim: The purpose of the present study was to investigate whether endothelial nitric oxide synthase (eNOS) gene polymorphisms play a role in prostate cancer (PCa). Materials and methods: We examined three eNOS gene polymorphisms (T-786C promoter region, G894T, and Intron 4 VNTR 4a/b) at extracted DNAs from 50 formalin-fixed paraffin-embedded tissues of PCa patients. For the controls, blood samples obtained from 50 healthy men were studied. Genotyping of molecular variants was performed by PCR-RFLP technique. Results: We found that the TC genotype of the T-786C polymorphism was associated with PCa risk (OR: 3.325, CI: 1.350-8.188, P = 0.008). The eNOS …

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Investigation Of Enteropathogenic Escherichia Coli And Shiga Toxin-Producing Escherichia Coli Associated With Hemolytic Uremic Syndrome In İzmir Province, Turkey, Eli̇f Bozçal, Gürkan Yi̇ği̇ttürk, Ataç Uzel, Sabi̇re Şöhret Aydemi̇r Jan 2016

Investigation Of Enteropathogenic Escherichia Coli And Shiga Toxin-Producing Escherichia Coli Associated With Hemolytic Uremic Syndrome In İzmir Province, Turkey, Eli̇f Bozçal, Gürkan Yi̇ği̇ttürk, Ataç Uzel, Sabi̇re Şöhret Aydemi̇r

Turkish Journal of Medical Sciences

Background/aim: The purpose of this study was to investigate Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains originating from diarrheagenic patients. Materials and methods: A total of 102 patients with diarrhea between October 2012 and January 2013 were enrolled in this study. Multiplex and standard polymerase chain reactions were performed to detect and distinguish STEC and EPEC strains. O serotyping of EPEC was carried out by monovalent antisera. The O and H serotyping of STEC strains was performed at the Refik Saydam Institute, Ankara. Results: A total of 5 (3.42%) strains were identified as STEC, and 3 …

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Levels Of Nitric Oxide, Asymmetric Dimethyl Arginine, Symmetric Dimethyl Arginine,And L-Arginine In Patients With Obsessive-Compulsive Disorder, Enver Demi̇rel Yilmaz, Mehmet Fati̇h Üstündağ, Ali̇ Görkem Gençer, Yüksel Kivrak, Özge Ünal, Mustafa Bi̇li̇ci̇ Jan 2016

Levels Of Nitric Oxide, Asymmetric Dimethyl Arginine, Symmetric Dimethyl Arginine,And L-Arginine In Patients With Obsessive-Compulsive Disorder, Enver Demi̇rel Yilmaz, Mehmet Fati̇h Üstündağ, Ali̇ Görkem Gençer, Yüksel Kivrak, Özge Ünal, Mustafa Bi̇li̇ci̇

Turkish Journal of Medical Sciences

Background/aim: We aimed to investigate and compare to healthy controls the variations in the levels of nitric oxide (NO), asymmetric dimethyl arginine (ADMA), symmetric dimethyl arginine (SDMA), and L-arginine levels in patients with obsessive-compulsive disorder (OCD). Materials and methods: We enrolled 30 patients with OCD and 30 healthy controls in the study consecutively. Diagnostic interviews of all participants were conducted with the Structured Clinical Interview for Axis I Disorders (SCID-I), and sociodemographic data of the participants were recorded. Patients scoring 10 points or more on the Yale-Brown Obsessive-Compulsive Scale were enrolled in the study. Results: The NO levels of patients …

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Nf-?? And Sod Expression In Preeclamptic Placentas, Abraham Silva Carmona, Hugo Mendieta Zeron Jan 2016

Nf-?? And Sod Expression In Preeclamptic Placentas, Abraham Silva Carmona, Hugo Mendieta Zeron

Turkish Journal of Medical Sciences

Background/aim: Preeclampsia is a leading cause of maternal death in the developing world. Our aim was to quantify and compare messenger (mRNA) expression of nuclear factor-kappa beta (NF-?β) and superoxide dismutase (SOD) in control patients with preeclampsia and without preeclampsia with or without familial hereditary background. Materials and methods: Four groups of patients were formed depending on the presence or absence of preeclampsia and presence or absence of familial history for preeclampsia. NF-?β and SOD were measured in human placentas by real-time quantitative polymerase chain reaction. The 2-ΔΔct analysis method was used to measure the difference in the relative expression …

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Prospects Of Curcumin As An Additive In Storage Solutions: A Study On Erythrocytes, Soumya Ravikumar, Carl Hsieh, Vani Rajashekharaiah Jan 2016

Prospects Of Curcumin As An Additive In Storage Solutions: A Study On Erythrocytes, Soumya Ravikumar, Carl Hsieh, Vani Rajashekharaiah

Turkish Journal of Medical Sciences

Background/aim: Curcumin, a naturally occurring antioxidant, shows a wide variety of medicinal properties. The possibility of utilizing curcumin as an additive in storage solutions of blood has not been explored. The purpose of this study was to analyze the effect of curcumin on erythrocytes during storage. Materials and methods: Blood obtained from rats was stored (4 °C) for 20 days in citrate-phosphate-dextrose-adenine-1 solution. Samples were divided into four groups: 1) Controls; 2) Curcumin 10 mM; 3) Curcumin 30 mM; and 4) Curcumin 60 mM. Every fifth day, hemoglobin, superoxide, antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase (GSH-Px)), lipid peroxidation …

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Pacaha Inhibits Proliferation Of Human Cancer Cells In Vitro, Sali̇ha Ekşi̇, Nebahat Ejder, Fati̇h Yilmaz, Ayşe Ertürk, Cemal Sandalli Jan 2016

Pacaha Inhibits Proliferation Of Human Cancer Cells In Vitro, Sali̇ha Ekşi̇, Nebahat Ejder, Fati̇h Yilmaz, Ayşe Ertürk, Cemal Sandalli

Turkish Journal of Medical Sciences

Background/aim: The aim of this study was to investigate the antiproliferative and cytotoxic effects of a newly synthesized molecule named paracetamol acetohydroxamic acid (PaCaHa) on human neoplastic cell lines. Materials and methods: A549, CRL 2923, HeLa, and ARPE were treated with various concentrations of PaCaHa and DMSO (vehicle control). The cytotoxic/cytostatic effects of PaCaHa were determined after a 24-h incubation period and compared to the DMSO control. The cytotoxic and antiproliferative effects were determined by the trypan blue dye exclusion and MTT methods. Results: A higher susceptibility to PaCaHA was found in CRL 2923 and HeLa cells, while A549 and …

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