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Nucleolus Assessment In Invasive Breast Carcinoma, An Objective Parameter For Histological Grading, Seda Duman Öztürk, Çi̇ğdem Öztürk, Oğuzhan Okcu, Bayram Şen, Recep Bedi̇r Jan 2022

Nucleolus Assessment In Invasive Breast Carcinoma, An Objective Parameter For Histological Grading, Seda Duman Öztürk, Çi̇ğdem Öztürk, Oğuzhan Okcu, Bayram Şen, Recep Bedi̇r

Turkish Journal of Medical Sciences

Background/aim: The nucleolus has the potential to provide insight into how many types of cancer will progress. In this study, we examined the evaluation of the nucleolus with a microscope in widespread breast cancer tumors and whether this value contributes to tumor grading as an objective clinicopathological parameter. Materials and methods: In our study, the nucleolus was evaluated retrospectively in resections with a diagnosis of invasive breast carcinoma of the cases between January 2010 and April 2021. In total, the tumor nucleolus of 377 cases of invasive breast carcinoma was evaluated. Nucleolus evaluation was performed with light microscopy using four …

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Use Of Ultrasonography In Confirming The Malposition Of The I-Gel In Pediatric Patients: A Prospective Observational Study, Faruk Çi̇çekci̇, Mehmet Selçuk Uluer, Mehmet Sargin, Emi̇ne Aslanlar, İnci̇ Kara, Muslu Kazim Körez Jan 2022

Use Of Ultrasonography In Confirming The Malposition Of The I-Gel In Pediatric Patients: A Prospective Observational Study, Faruk Çi̇çekci̇, Mehmet Selçuk Uluer, Mehmet Sargin, Emi̇ne Aslanlar, İnci̇ Kara, Muslu Kazim Körez

Turkish Journal of Medical Sciences

Background/aim: This study was designed to observe and compare the performance of fiberoptic laryngeal (FOL) view, leakage test (LT) and ultrasonography (USG) usage in detecting i-gel position in pediatric patients. Materials and methods: One hundred ten consecutive children were included in this single-armed prospective observational study. After anesthetic induction, i-gel placement confirmed using FOL and LT was evaluated using USG in three planes. According to our scoring system, acceptable and unacceptable grades (FOL, LT and USG) were determined to describe placement. Sensitivity and specificity were determined by comparing USG performance with the other two tests. Results: Ultrasonography was found a …

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Could Lysosomal Acid Lipase Enzyme Activity Be Used For Clinical Follow-Up In Cryptogenic Cirrhosis?, Engi̇n Köse, Elçi̇n Çağatay, Tutku Yaraş Mercan, Peli̇n Teke Kisa, Semi̇nay Güler, Zümrüt Arslan Gülten, Mesut Akarsu, Yavuz Oktay, Hülya Ayar Kayali, Nur Arslan Jan 2022

Could Lysosomal Acid Lipase Enzyme Activity Be Used For Clinical Follow-Up In Cryptogenic Cirrhosis?, Engi̇n Köse, Elçi̇n Çağatay, Tutku Yaraş Mercan, Peli̇n Teke Kisa, Semi̇nay Güler, Zümrüt Arslan Gülten, Mesut Akarsu, Yavuz Oktay, Hülya Ayar Kayali, Nur Arslan

Turkish Journal of Medical Sciences

Background/aim: Cholesterol ester storage disease (CESD) is one of the rare causes that should be kept in mind in the etiology of cirrhosis. Recent studies detected that significantly reduced lysosomal acid lipase deficiency enzyme (LAL) in patients with cryptogenic cirrhosis (CC). Moreover, studies have evaluated that LAL activity is as effective as scoring systems in assessing the severity of cirrhosis. In this study, we aimed to investigate the CESD with LAL level and mutation analysis of LIPA gene in patients diagnosed with CC and to compare LAL activities between patients with CC and healthy volunteers. Materials and methods: Laboratory parameters …

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The Clinical And Demographical Characteristics Of Turkish Pediatric Lymphedema Patients: A Multicenter Study, Pinar Borman, Ayşegül Balcan, Si̇bel Eyi̇gör, Evri̇m Coşkun, Fi̇kri̇ye Fi̇gen Ayhan, Burcu Duyur Çakit, Seçi̇l Vural, Meltem Vural, Esra Deni̇z Papatya Çakir, Deni̇z Nazi̇re Çağdaş Ayvaz, Ayşegül Yaman, Ayşe Beyhan Lale Cerrahoğlu, Sevi̇l Ceyhan Doğan Jan 2022

The Clinical And Demographical Characteristics Of Turkish Pediatric Lymphedema Patients: A Multicenter Study, Pinar Borman, Ayşegül Balcan, Si̇bel Eyi̇gör, Evri̇m Coşkun, Fi̇kri̇ye Fi̇gen Ayhan, Burcu Duyur Çakit, Seçi̇l Vural, Meltem Vural, Esra Deni̇z Papatya Çakir, Deni̇z Nazi̇re Çağdaş Ayvaz, Ayşegül Yaman, Ayşe Beyhan Lale Cerrahoğlu, Sevi̇l Ceyhan Doğan

Turkish Journal of Medical Sciences

Background/aim: Reducing lymphedema-associated burden and disability in the pediatric setting requires improved awareness and understanding clinical properties of the lymphedema. The aim of this study was to evaluate the clinical and demographic characteristics of patients with pediatric lymphedema presented to different lymphedema centers in Turkey. Materials and methods: The socio-demographic and clinical characteristics of the children including age, gender, presence of genetic syndromes, duration of edema, site and stage of lymphedema and the received therapies were determined. Parental and children education on self-management techniques were recorded. Results: A total of 122 children (female: 66, male: 56) with a mean age …

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Stable And Exacerbation Period Serum Cytokine And Periostin Levels Of The Five Distinct Phenotypes Of Severe Asthma, Murat Türk, İnsu Yilmaz, Selma Gökahmetoğlu, Ayşe Nedret Koç Jan 2022

Stable And Exacerbation Period Serum Cytokine And Periostin Levels Of The Five Distinct Phenotypes Of Severe Asthma, Murat Türk, İnsu Yilmaz, Selma Gökahmetoğlu, Ayşe Nedret Koç

Turkish Journal of Medical Sciences

Background/aim: The differences in molecular mechanisms during a stable period and the changes in the inflammatory responses during exacerbations between distinct severe asthma phenotypes remain unclear. In this study, we aimed to characterize stable and exacerbation period serum cytokine and periostin levels of 5 different predefined severe asthma phenotypes with real-life data. Changes in the viral infection-induced exacerbations were also analyzed. Materials and methods: Serum levels of 8 cytokines and periostin were measured from the sera obtained from the adult patients with five different severe asthma phenotypes based on the presence/absence of aeroallergen sensitivity, peripheral eosinophilia and chronic rhinosinusitis with …

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Analysis Of The Relationship Between Tuberculosis-Related Mortality And Nitrous Oxide Emission Levels In The World With The Environmental Kuznets Curve Method, Şeri̇fe Torun, Kadi̇r Yilmaz, Şevket Özkaya, Şebnem Yosunkaya, Müşerref Şule Akçay Jan 2022

Analysis Of The Relationship Between Tuberculosis-Related Mortality And Nitrous Oxide Emission Levels In The World With The Environmental Kuznets Curve Method, Şeri̇fe Torun, Kadi̇r Yilmaz, Şevket Özkaya, Şebnem Yosunkaya, Müşerref Şule Akçay

Turkish Journal of Medical Sciences

Background/aim: It was aimed to analyze the relationship between tuberculosis-related mortality and nitrous oxide emission levels in the world with the Environmental Kuznets Curve (EKC) Method. Materials and methods: WHO ICD-10 mortality list data and the World Bank Country Data (WBCD) were used between 1997 and 2017 for 12 countries. Cubic regression analysis was used for EKC Analysis. Results: The difference between male and female deaths between 1996 and 1998 has increased sharply since 1999. Male deaths consistently occurred significantly more than female deaths. There was a significant and negative correlation between Nitrous oxide emissions (% change from 1990) and …

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Egfr Blocker Lapatinib Inhibits The Synthesis Of Matrix Metalloproteinases From Synovial Fibroblasts, Demet Yalçin Kehri̇bar, Hakan Emmungi̇l, Neşe Başak Türkmen, Osman Çi̇ftçi̇, Emi̇ne Şalva, Meti̇n Özgen Jan 2022

Egfr Blocker Lapatinib Inhibits The Synthesis Of Matrix Metalloproteinases From Synovial Fibroblasts, Demet Yalçin Kehri̇bar, Hakan Emmungi̇l, Neşe Başak Türkmen, Osman Çi̇ftçi̇, Emi̇ne Şalva, Meti̇n Özgen

Turkish Journal of Medical Sciences

Background/aim: Epidermal growth factor receptor (EGFR) family members and their associated ligands may be related to bone and joint destruction in rheumatoid arthritis. Matrix metalloproteinases are responsible for joint and bone tissue degradation. This study is intended to investigate the effect of epidermal growth factor receptor inhibition by lapatinib on the synthesis of matrix metalloproteinases in in vitro. Materials and methods: Synovial fibroblast cell culture was obtained from a patient with rheumatoid arthritis who underwent knee arthroplasty. Interleukin-1ß (IL-1ß) and tumor necrosis factor-∝ (TNF-∝) were added to the cell culture to stimulate synovial fibroblast cells and create an inflammatory character. …

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Alpha-Lipoic Acid Prevents Atrial Electrical And Structural Remodeling Via Inhibition Of Nadph Oxidase In A Rabbit Rapid Atrial Pacing Model, Lei Chen, Wen Gao, Yameng Shao, Chenggang Li, Yuan Lu Jan 2022

Alpha-Lipoic Acid Prevents Atrial Electrical And Structural Remodeling Via Inhibition Of Nadph Oxidase In A Rabbit Rapid Atrial Pacing Model, Lei Chen, Wen Gao, Yameng Shao, Chenggang Li, Yuan Lu

Turkish Journal of Medical Sciences

Background/aim: Alpha-lipoic acid (ALA) is a natural compound, one of the natural antioxidants with high activity. In the NADPH oxidase family, NADPH oxidase 4 (NOX4) is an important subunit participating in the production of ROS. NADPH oxidase 2 (NOX2) can form active NADPH oxidase complexes when binding to several other subunits in the cytoplasm, and NOX2 is its major functional subunit. Rapid atrial pacing (RAP) model was constructed to study the effects of ALA on electrical and structural remodeling in rabbits. Materials and methods: Thirty rabbits were divided into SHAM group, RAP group and ALA+RAP group. Their right atriums were …

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Microglial Iron Trafficking: New Player In Brain Injury, Elif Keles, Hasan Hüseyi̇n Kazan, Lati̇fe Arzu Aral, Hayrunni̇sa Bolay Belen Jan 2022

Microglial Iron Trafficking: New Player In Brain Injury, Elif Keles, Hasan Hüseyi̇n Kazan, Lati̇fe Arzu Aral, Hayrunni̇sa Bolay Belen

Turkish Journal of Medical Sciences

Neonatal brain injury is a significant reason of neurodevelopmental abnormalities and long-term neurological impairments. Hypoxic-ischemic encephalopathy and preterm brain injury, including intraventricular hemorrhage are the most common grounds of brain injury for full-term and preterm neonates. The prevalence of hypoxic ischemic encephalopathy varies globally, ranging from 1 to 3.5/1000 live births in high-resource countries and 26/1000 in low-resource countries. Preterm birth's global incidence is 15 million, a significant reason for infant mortality and morbidity, permanent neurologic problems, and the associated social and economic burden. The widespread neurodevelopmental effects of neonatal brain injury could have an unfavorable impact on a variety …

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Identification Of Sepsis-Associated Encephalopathy Risk Factors In Elderly Patients: A Retrospective Observational Cohort Study, Guangyong Jin, Shengyun Wang, Jiayi Chen, Wei Hu, Ying Zhu, Shaosong Xi Jan 2022

Identification Of Sepsis-Associated Encephalopathy Risk Factors In Elderly Patients: A Retrospective Observational Cohort Study, Guangyong Jin, Shengyun Wang, Jiayi Chen, Wei Hu, Ying Zhu, Shaosong Xi

Turkish Journal of Medical Sciences

Background/aim: Sepsis-associated encephalopathy (SAE) is a severe complication of sepsis that affects upwards of half of all sepsis patients. Few studies have examined the etiology and risk factors of SAE among elderly patients. This study was designed to explore the epidemiology of SAE and the risk factors associated with its development in elderly populations. Materials and methods: This was a retrospective analysis of elderly sepsis patients admitted to our intensive care unit between January 2017 and January 2022. We then compared non-SAE and SAE groups concerning baseline clinicopathological findings, underlying diseases, infection site, disease type, disease severity, biochemical findings, and …

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Is Microcystic, Elongated, And Fragmented Pattern Of Myometrial Invasion In Endometrioid Endometrial Carcinoma Associated With Survival?, Sultan Deni̇z Altindağ, Seyran Yi̇ği̇t, Serhat Şen Jan 2022

Is Microcystic, Elongated, And Fragmented Pattern Of Myometrial Invasion In Endometrioid Endometrial Carcinoma Associated With Survival?, Sultan Deni̇z Altindağ, Seyran Yi̇ği̇t, Serhat Şen

Turkish Journal of Medical Sciences

Background/aim: Microcystic, elongated, and fragmented (MELF) pattern is one of the myometrial invasion patterns in endometrioid endometrial carcinoma (EEC), and it has been associated with poor prognostic parameters, especially lymphovascular space invasion (LVSI) and lymph node metastasis (LNM). This study aims to represent the frequency of MELF pattern in EEC and the relationship between MELF pattern with clinicopathological parameters, overall survival (OS), and disease-free survival (DFS). Materials and methods: In this retrospective cohort study, we examined 233 patients with EEC diagnoses with the result of a total hysterectomy and bilateral salpingo-oophorectomy between 2009 and 2014. The association of MELF pattern …

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Evaluation Of The Effect Of Hyperchloremia On The Prognosis And Mortality Of Medical Intensive Care Patients: A Single-Center Study, Habi̇p Yilmaz, Başak Çakir Güney Jan 2022

Evaluation Of The Effect Of Hyperchloremia On The Prognosis And Mortality Of Medical Intensive Care Patients: A Single-Center Study, Habi̇p Yilmaz, Başak Çakir Güney

Turkish Journal of Medical Sciences

Background/aim: While chloride (Cl) is the most abundant anion in the serum, it is unfortunately one of the most commonly disregarded laboratory test results routinely drawn upon admission into the medical intensive care unit (MICU). We aimed to investigate the relation between in-hospital mortality, different pathologies requiring admission to the MICU, serum Cl levels, and other biochemical tests in a tertiary center. Materials and methods: The prospective study included data from 373 patients admitted to the ICU of a tertiary care center between 2017 and 2019. Data of patients under 18, pregnant patients or patients who were in the MICU …

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The Effect Of Topically Administered Lavender Aromatherapy On The Pain Of Insulin Injection In Diabetic Patients: A Double-Blind Randomized Controlled Clinical Trial, Hati̇ce Demi̇rağ, Sevi̇lay Hi̇nti̇stan, Enes Bulut Jan 2022

The Effect Of Topically Administered Lavender Aromatherapy On The Pain Of Insulin Injection In Diabetic Patients: A Double-Blind Randomized Controlled Clinical Trial, Hati̇ce Demi̇rağ, Sevi̇lay Hi̇nti̇stan, Enes Bulut

Turkish Journal of Medical Sciences

Background/aim: Needle phobia occurs in more than half of diabetic patients due to the pain caused by frequent insulin injections. Therefore, this study evaluated the effect of topically administered lavender aromatherapy on the pain of insulin injections in diabetic patients. Materials and methods: In this double-blind randomized controlled and experimental study, patients who met the study criteria were divided into three groups; topical lavender oil (n = 60), placebo (n = 60), and control (n = 60) groups. The data were collected using the "Patient Information Form", the "Follow-up Form", the "Verbal Category Scale (VCS)", and the "Visual Analogue Scale …

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Clinicoradiological Evaluation Of Oxidative Stress Activity In Acute Cerebral Infarction In The First 24 H And The Qualitative Importance Of Dysfunctional Hdl In Stroke, Esra Demi̇r Ünal, Gönül Vural, Funda Eren, Sali̇m Neşeli̇oğlu, Özcan Erel Jan 2022

Clinicoradiological Evaluation Of Oxidative Stress Activity In Acute Cerebral Infarction In The First 24 H And The Qualitative Importance Of Dysfunctional Hdl In Stroke, Esra Demi̇r Ünal, Gönül Vural, Funda Eren, Sali̇m Neşeli̇oğlu, Özcan Erel

Turkish Journal of Medical Sciences

Background/aim: Acute cerebral infarction (ACI) occurs as a result of instant disruption of vascular flow that causes disbalance between oxidative/antioxidative activity. We examined the relationship of serum neuro-oxidative stress parameters with stroke severity and infarct volume in ACI and emphasized the qualitative importance of high-density lipoprotein (HDL) on its relationship with myeloperoxidase (MPO) and paraoxonase-1 (PON1) in the acute period of stroke. Materials and methods: One hundred ACI patients applied within the first 24 h and 50 healthy volunteers were included. The patient group was evaluated with demographic data (including arrival serum biochemical assessment), clinical disability scores, infarct volume, serum …

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Phytochemical Constituents Of Aquilaria Malaccensis Leaf Extract And Their Anti-Inflammatory Activity Against Lps/Ifn-Γ-Stimulated Raw 264.7 Cell Line, Eman Ramadan, Manar A. Eissa, Yumi Z. H-Y Hashim, Dina M. El-Kersh Jan 2022

Phytochemical Constituents Of Aquilaria Malaccensis Leaf Extract And Their Anti-Inflammatory Activity Against Lps/Ifn-Γ-Stimulated Raw 264.7 Cell Line, Eman Ramadan, Manar A. Eissa, Yumi Z. H-Y Hashim, Dina M. El-Kersh

Pharmacy

This study aims to identify the major phytochemical constituents in Aquilaria malaccensis (Thymelaeaceae) ethanolic leaf extract (ALEX-M) and elucidate their ability to suppress nitric oxide (NO) production from a murine macrophage-like cell line (RAW 264.7) stimulated by lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Dichloromethane (DCM) and ethyl acetate (EtOAc) fractions of ALEX-M were subjected to column chromatography. Eight known compounds were isolated for the first time from this species. Compounds were identified using spectroscopic techniques (IR, UV, HRESIMS, and 1D and 2D NMR). Anti-inflammatory activity of both extract and isolated compounds were investigated in vitro. The fractions offered the isolation of …

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Exertional Heat Stroke In A Male High School Runner With Disordered Eating: A Disablement Model Case Study, Brittni G. Hoover, Rebecca M. Hirschhorn, Allison B. Smith, Amy F. Hand Jan 2022

Exertional Heat Stroke In A Male High School Runner With Disordered Eating: A Disablement Model Case Study, Brittni G. Hoover, Rebecca M. Hirschhorn, Allison B. Smith, Amy F. Hand

Clinical Practice in Athletic Training

A 16-year-old male high school cross country athlete collapsed at the end of an afternoon 5K cross country meet. The patient was unable to stand, and after outwardly displaying confusion and agitation, his coaches sought assistance from the athletic trainers (ATs). The patient consumed one cup of water and a granola bar on the day of the meet. The ambient air temperature was 90°F with 69% humidity. Differential diagnoses included exertional heat stroke (EHS), exertional heat exhaustion, exertional collapse associated with sickle cell trait, heat syncope, dehydration, malnutrition, and hypoglycemia. Approximately 20 minutes passed between activation of the emergency action …

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Characteristics Of Sickle Cell Trait Policies And Procedures At Ncaa Division Iii Institutions, M A. Eilers, B J. Warner, D M. Gallegos, J A. Clark, S A. Cage Jan 2022

Characteristics Of Sickle Cell Trait Policies And Procedures At Ncaa Division Iii Institutions, M A. Eilers, B J. Warner, D M. Gallegos, J A. Clark, S A. Cage

Clinical Practice in Athletic Training

Context: In 2010, the National Collegiate Athletic Association (NCAA) introduced a proposal addressing sickle cell trait screening. This proposal later became a policy that required student-athletes in their first year of eligibility to provide their institution with accurate information regarding their sickle cell trait status. There does not appear to be a recent study published that has examined the sickle cell trait screening policy and procedures currently being used by NCAA Division III Institutions. Thus, the purpose of this study was to describe the characteristics of sickle cell trait policies and procedures at NCAA Division III institutions. Methods: We used …

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Autonomous And Non-Autonomous Requirements For The C-Jun N-Terminal Kinase Signaling Pathway In Early Forebrain Development, Jessica G. Cunningham Jan 2022

Autonomous And Non-Autonomous Requirements For The C-Jun N-Terminal Kinase Signaling Pathway In Early Forebrain Development, Jessica G. Cunningham

Graduate Theses, Dissertations, and Problem Reports (ETD)

The cerebral cortex is responsible for a wide variety of high-level functions including cognition, sensory perception, fine motor control, and the orchestration of body movements. The cortex is comprised of cortical excitatory neurons and inhibitory interneurons, which are arranged in a highly organized fashion into different layers and regions. These two types of cells operate in a delicate balance between excitation and inhibition, which is critical for proper cortical circuitry. In order for the cortex to execute its numerous functions, it must both send and receive input to other brain regions through axonal connections. The organization within the cortex and …

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Evaluation Of Ivermectin Antiviral Activity Against Avian Infectious Bronchitis Virus Using A Chicken Embryo Model, Donald L. Reynolds, E. Barry Simpson Jan 2022

Evaluation Of Ivermectin Antiviral Activity Against Avian Infectious Bronchitis Virus Using A Chicken Embryo Model, Donald L. Reynolds, E. Barry Simpson

School of Veterinary and Biomedical Sciences: Faculty Publications

Ivermectin is widely used in both animals and humans as an FDA-approved parasiticide. Ivermectin has also been reported to have antiviral activity against several viruses including coronaviruses. There are reports that indicate ivermectin may have some role in diminishing the disease caused by SARS-CoV-2, but the evidence is inconclusive. The objective of this study was to determine if ivermectin was efficacious in inhibiting avian infectious bronchitis virus (IBV, a coronavirus) replication in chicken embryos. Briefly, our approach was to use the Massachusetts vaccine strain of IBV in combination with various doses of ivermectin and then inoculate these preparations into chicken …

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Rna Nanovaccine Protects Against White Spot Syndrome Virus In Shrimp, Yashdeep Phanse, Supraja Puttamreddy, Duan Loy, Julia Vela Ramirez, Kathleen A. Ross, Ignacio Alvarez-Castro, Mark Mogler, Scott Broderick, Krishna Rajan, Balaji Narasimhan, Lyric C. Bartholomay Jan 2022

Rna Nanovaccine Protects Against White Spot Syndrome Virus In Shrimp, Yashdeep Phanse, Supraja Puttamreddy, Duan Loy, Julia Vela Ramirez, Kathleen A. Ross, Ignacio Alvarez-Castro, Mark Mogler, Scott Broderick, Krishna Rajan, Balaji Narasimhan, Lyric C. Bartholomay

School of Veterinary and Biomedical Sciences: Faculty Publications

In the last 15 years, crustacean fisheries have experienced billions of dollars in economic losses, primarily due to viral diseases caused by such pathogens as white spot syndrome virus (WSSV) in the Pacific white shrimp Litopenaeus vannamei and Asian tiger shrimp Penaeus monodon. To date, no effective measures are available to prevent or control disease outbreaks in these animals, despite their economic importance. Recently, double-stranded RNA-based vaccines have been shown to provide specific and robust protection against WSSV infection in cultured shrimp. However, the limited stability of double-stranded RNA is the most significant hurdle for the field application of these …

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The Application Of The Skin Virome For Human Identification, Ema H. Graham, Jennifer Clarke, Samodha Fernando, Joshua Herr, Michael Adamowicz Jan 2022

The Application Of The Skin Virome For Human Identification, Ema H. Graham, Jennifer Clarke, Samodha Fernando, Joshua Herr, Michael Adamowicz

Nebraska Center for Virology: Faculty Publications

The use of skin virome offers a unique approach for human identification purposes in instances where a viable and statistically relevant human DNA profile is unavailable. The skin virome may act as an alternative DNA profile and/or an additional form of probative genetic material. To date, no study has attempted to investigate the human virome over a time series across various physical locations of the body to identify its diagnostic potential as a tool for human identification. For this study, we set out to evaluate the stability, diversity, and individualization of the human skin virome. An additional goal was to …

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Immunogenicity And Protective Efficacy Of A Recombinant Pichinde Viral-Vectored Vaccine Expressing Influenza Virus Hemagglutinin Antigen In Pigs, Sushmita Kumari, Jayeshbhai Chaudhari, Qinfeng Huang, Phillip Gauger, Marcelo Nunes De Almeida, Yuying Liang, Hinh Ly, Hiep Vu Jan 2022

Immunogenicity And Protective Efficacy Of A Recombinant Pichinde Viral-Vectored Vaccine Expressing Influenza Virus Hemagglutinin Antigen In Pigs, Sushmita Kumari, Jayeshbhai Chaudhari, Qinfeng Huang, Phillip Gauger, Marcelo Nunes De Almeida, Yuying Liang, Hinh Ly, Hiep Vu

Nebraska Center for Virology: Faculty Publications

Influenza A virus of swine (IAV-S) is an economically important swine pathogen. The IAV-S hemagglutinin (HA) surface protein is the main target for vaccine development. In this study, we evaluated the feasibility of using the recombinant tri-segmented Pichinde virus (rPICV) as a viral vector to deliver HA antigen to protect pigs against IAV-S challenge. Four groups of weaned pigs (T01–T04) were included in the study. T01 was injected with PBS to serve as a non-vaccinated control. T02 was inoculated with rPICV expressing green fluorescence protein (rPICV-GFP). T03 was vaccinated with rPICV expressing the HA antigen of the IAV-S H3N2 strain …

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Fertility Control Options For Management Of Free-Roaming Horse Populations, Ursula S. Bechert, John W. Turner Jr., Dan L. Baker, Douglas C. Eckery, Jason E. Bruemmer, Candace C. Lyman, Tulio M. Prado, Sarah R. B. King, Mark A. Fraker Jan 2022

Fertility Control Options For Management Of Free-Roaming Horse Populations, Ursula S. Bechert, John W. Turner Jr., Dan L. Baker, Douglas C. Eckery, Jason E. Bruemmer, Candace C. Lyman, Tulio M. Prado, Sarah R. B. King, Mark A. Fraker

Human–Wildlife Interactions

The management of free-roaming horses (Equus ferus) and burros (E. asinus) in the United States has been referred to as a “wicked problem” because, although there are population control options, societal values will ultimately determine what is acceptable and what is not. In the United States, free-roaming equids are managed by different types of organizations and agencies, and the landscapes that these animals inhabit vary widely in terms of access, size, topography, climate, natural resources, flora, and fauna. This landscape diversity, coupled with contemporary socioeconomic and political environments, means that adaptive management practices are needed to …

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Exploring Roles Of Mir-885-5p In Hepatocellular Carcinoma, Archittapon Nokkeaw Jan 2022

Exploring Roles Of Mir-885-5p In Hepatocellular Carcinoma, Archittapon Nokkeaw

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hepatocellular carcinoma (HCC), the most prevalent form of liver cancer, exerts a significant burden on Southeast Asian countries and stands as the third leading cause of cancer-related mortality worldwide. Despite this alarming impact, effective treatments for HCC are lacking, resulting in low survival rates and high recurrence rates. Therefore, a comprehensive understanding of the disease's underlying mechanisms is crucial for the development of novel and potent therapies. Recently, it has been recognized that microRNAs (miRNAs) play a vital role in tumorigenesis, including HCC. Our bioinformatic analysis has highlighted hsa-miR-885-5p as a potential candidate miRNA due to its downregulation in HCC …

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Exploring Roles Of Mir-372-3p In Proliferation Of Hepatocellular Carcinoma Cells, Pannathon Thamjamrassri Jan 2022

Exploring Roles Of Mir-372-3p In Proliferation Of Hepatocellular Carcinoma Cells, Pannathon Thamjamrassri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer. HCC has a replicative immortality and sustained proliferation rate. In addition, cell cycle-related protein regulating proliferation in cancer are often found dysregulated, allowing cancer cells to proceed their proliferation uncontrollably. Recently, a small non-coding RNA, microRNA (miRNA), was found to play an important role in numerous biological functions. Specific miRNA may ameliorate or promote cancer progression through different target mRNA. MiR-372-3p has been explored in various cancers such as colon cancer, colorectal cancer, and glioma. However, its functions have been rarely studied in HCC, especially in the aspect …

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Effects Of Chitosan Oligosaccharide And Probiotics On Chronic Kidney Disease Rats, Weerapat Anegkamol Jan 2022

Effects Of Chitosan Oligosaccharide And Probiotics On Chronic Kidney Disease Rats, Weerapat Anegkamol

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chronic kidney disease (CKD) patients suffer from the accumulation of toxic substances in their blood due to the loss of kidney function, which results in hyperphosphatemia. This condition contributes to hyperparathyroidism, leading to the development of chronic kidney disease-related mineral bone disorder (CKD-MBD). Additionally, CKD patients experience changes in their gut microbiota, disrupting epithelial tight junctions and allowing excessive absorption of dietary phosphate. In this study, we aimed to investigate the effects of various oligosaccharides and probiotics on the gut microbiota, intestinal barrier, hyperphosphatemia, and hyperparathyroidism in CKD rats. We isolated Lactobacillus and Bifidobacterium strains from healthy participants and tested …

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Study Of Thermodynamic Binding Between Ader And Ades In Two-Component Antibiotic Sensor Of Antibiotic Resistant Strains Of Acinetobacter Baumannii, Konrawee Thananon Jan 2022

Study Of Thermodynamic Binding Between Ader And Ades In Two-Component Antibiotic Sensor Of Antibiotic Resistant Strains Of Acinetobacter Baumannii, Konrawee Thananon

Chulalongkorn University Theses and Dissertations (Chula ETD)

The proteins AdeR (response regulator) and AdeS (histidine kinase sensor) play a crucial role in regulating the efflux pump, which is responsible for removing antibiotics from bacterial cells. This regulation occurs through a two-component regulatory system (TCS) involving autophosphorylation. Upon receiving external signals, AdeS undergoes autophosphorylation, leading to the activation of AdeR, which in turn initiates the expression of target genes related to the efflux pump. In this study, both enzymes from the reference strain of Acinetobacter baumannii (ATCC19606) and antibiotic resistant strains (H1074 and G560) were cloned and expressed in E. coli. The enzymes were purified using an immobilized …

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การศึกษารูปแบบการแสดงออกของโปรตีนที่เกี่ยวข้องกับมะเร็งในเนื้องอกโพรงจมูกและไซนัสชนิดอินเวอร์เต็ดแปปิลโลมาที่มีลักษณะทางพยาธิสภาพผิดปกติด้วยการย้อมอิมมูโนฮิสโตเคมี, ธมนวรรณ มีสกุล Jan 2022

การศึกษารูปแบบการแสดงออกของโปรตีนที่เกี่ยวข้องกับมะเร็งในเนื้องอกโพรงจมูกและไซนัสชนิดอินเวอร์เต็ดแปปิลโลมาที่มีลักษณะทางพยาธิสภาพผิดปกติด้วยการย้อมอิมมูโนฮิสโตเคมี, ธมนวรรณ มีสกุล

Chulalongkorn University Theses and Dissertations (Chula ETD)

เนื้องอกโพรงจมูกและไซนัสชนิดอินเวอร์เต็ดแปปิลโลมา (ISP) เป็นเนื้องอกชนิดไม่รุนแรงที่พบได้บ่อยที่สุดในโพรงจมูกและไซนัส จากการสังเกตของพยาธิแพทย์ผู้ร่วมวิจัยบางท่านพบว่า เนื้องอก ISP ที่มีเซลล์ชนิดสความัสเป็นส่วนประกอบมีแนวโน้มรุนแรงมากกว่า ดังนั้นในงานวิจัยนี้ ผู้วิจัยจึงมีความพยายามวิเคราะห์กลุ่มเนื้องอกนี้อย่างละเอียด โดยตั้งชื่อว่า เนื้องอกอินเวอร์เต็ดแปปิลโลมาที่มีลักษณะทางพยาธิสภาพผิดปกติ (ASP) โดยที่ ASP มีส่วนประกอบเป็นเซลล์ชนิดสความัสเป็นส่วนประกอบ ซึ่งมีความคล้ายคลึงกับมะเร็งชนิดสความัสเซลล์คาร์สิโนมา (SCC) ผู้วิจัยจึงมีสมมุติฐานว่าอาจมีความเป็นไปได้ที่เนื้องอก ASP จะเป็นรอยโรคก่อนที่จะพัฒนาไปเป็นมะเร็ง โดยในงานวิจัยนี้ผู้วิจัยได้วิเคราะห์การแสดงออกของโปรตีน 10 ชนิดที่เกี่ยวข้องกับมะเร็งด้วยการย้อมอิมมูโนฮิสโตเคมี และใช้โปรแกรมวิเคราะห์ภาพแบบดิจิทัลในการประเมินระดับการแสดงออกในรอยโรค ISP ASP และ SCC จากการศึกษาพบว่า เนื้องอก ASP มีระดับการแสดงออกของโปรตีน Ki67 และ EGFR ที่แตกต่างกับกลุ่มรอยโรคอื่น ๆ อย่างมีนัยสำคัญคือ มีการแสดงออกของ Ki67 มากกว่าในเนื้องอก ISP (p = 0.0054) และมีการแสดงออกของ EGFR ต่ำกว่า SCC (p = 0.0110) อีกทั้งมีแนวโน้มของจำนวนการแบ่งเซลล์ชนิดไมโทซิสเพิ่มขึ้นในรอยโรค ISP ASP และ SCC ตามลำดับอย่างมีนัยสำคัญ (p

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Roles Of Mtor Complex 2 In Canonical Wnt Signaling In Glioblastoma., Tankun Banlue Jan 2022

Roles Of Mtor Complex 2 In Canonical Wnt Signaling In Glioblastoma., Tankun Banlue

Chulalongkorn University Theses and Dissertations (Chula ETD)

Glioblastoma (GBM) is the most common type of primary brain tumor in adults, comprising 75% of cases. The failure of GBM treatments is closely related to a subset of cells known as glioblastoma stem cells (GSCs), which possess stemness characteristics. While the Wnt signaling pathway plays a crucial role in regulating stem cell homeostasis, the interplay between this pathway and the mTORC2 signaling pathway in GBM remains unclear. Thus, this research aimed to investigate the collaborative regulation of stemness-related mechanisms by mTORC2 and canonical Wnt signaling pathways in glioblastoma using 3D spheroid culture. The study found that inhibiting mTORC2 resulted …

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Plasma Levels And Association Of Phosphorylated Tau 181, 217 And 231 With Cognitive Impairment In Elderly Thai Population, Pasin Hemachudha Jan 2022

Plasma Levels And Association Of Phosphorylated Tau 181, 217 And 231 With Cognitive Impairment In Elderly Thai Population, Pasin Hemachudha

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background The identification of biomarkers for Alzheimer's disease (AD) in blood samples has the potential to facilitate early diagnosis and improve the accuracy of AD diagnosis. Plasma phosphorylated tau (p-tau) is a promising biomarker for AD with an accuracy potentially comparable to a cerebrospinal sample; however, its utility in the clinical setting has yet to be explored in Thailand. The present study aimed to evaluate the utility of p-tau 181 as a tool for screening cognitive impairment in a non-demented population, estimating the prevalence of AD and investigating its potential as a risk stratification strategy. Method The study recruited 254 …

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