Digital Commons Network^™

Isolation And Immunological Characterization Of A Group Of New B Lymphomas From Cba Mice, Venkatachalam Udhayakumar, Peter H. Brodeur, M. S. Rajagopalan, Stephen Zimmer, Karen E. Pollok, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

We have isolated and characterized a new series of B lymphomas which occurred spontaneously in a group of CBA N mice that were transferred with spleen or lymph node cells from 24-month-old CBA Ca mice. Tumor cell lines from six CBA N mice that received spleen cells were rescued and designated as BKS-2, BKS-3, BKS-4, BKS-5, BKS-6, and BKS-7. Also, tumor cells from a recipient of lymph node cells were rescued and the resulting cell line was designated BKL. These tumor cells expressed membrane immunoglobulin (μ,κ), major histocompatibility complex Class I and Class II molecules, B220, Lyb8, Fc receptors, J11d, …

Identification Of Persistent Defects In Insulin Receptor Structure And Function In Capillary Endothelial Cells From Diabetic Rats, Ching Fai Kwok, Barry J. Goldstein, Dirk Muller-Wieland, Tian-Shing Lee, C. Ronald Kahn, George L. King

Department of Medicine Faculty Papers

Insulin actions and receptors were studied in capillary endothelial cells cultured from diabetic BB rats and their nondiabetic colony mates. The endothelial cells from diabetic rats of 2 mo duration had persistent biological and biochemical defects in culture. Compared with normal rats, endothelial cells from diabetic rats grew 44% more slowly. Binding studies of insulin and insulin-like growth factor I (IGF-I) showed that cells from diabetic rats had 50% decrease of insulin receptor binding (nondiabetic: 4.6 +/- 0.7; diabetic: 2.6 +/- 0.4% per milligram protein, P < 0.01), which was caused by a 50% decrease in the number of binding sites per milligram protein, whereas IGF-I binding was not changed. Insulin stimulation of 2-deoxy-glucose uptake and alpha-aminoisobutyric acid uptake were also severely impaired with a 80-90% decrease in maximal stimulation, in parallel with a 62% decrease in insulin-stimulated autophosphorylation (P < 0.05). ¹²⁵I-insulin cross-linking revealed an 140-kD alpha subunit of the insulin receptor similar to …

Passive Cation Transport In Reversibly Sickling Deer Red Blood Cells, Norma C. Adragna, Peter K. Lauf

Pharmacology and Toxicology Faculty Publications

No abstract provided.

Insulin Stimulates The Dephosphorylation And Activation Of Acetyl-Coa Carboxylase., Lee A. Witters, Thomas D. Watts, Diana L. Daniels, Joseph L. Evans

Dartmouth Scholarship

The mechanism underlying the ability of insulin to acutely activate acetyl-CoA carboxylase [acetyl-CoA: carbon-dioxide ligase (ADP-forming), EC 6.4.1.2; AcCoA-Case] has been examined in Fao Reuber hepatoma cells. Insulin promotes the rapid activation of AcCoACase, as measured in cell lysates, and this stimulation persists to the same degree after isolation of AcCoACase by avidin-Sepharose chromatography. The insulin-stimulated enzyme, as compared with control enzyme, exhibits an increase in both citrate-independent and -dependent activity and a decrease in the Ka for citrate. Direct examination of the phosphorylation state of isolated 32P-labeled AcCoACase after insulin exposure reveals a marked decrease in total enzyme phosphorylation …

K+ Efflux In Nih Mouse 3t3 Cells And Transformed Derivatives: Dependence On Extracellular Ca2+ And Phorbol Esters., Martin Lubin

Dartmouth Scholarship

In culture medium deficient in Ca2+, NIH mouse 3T3 cells lose K+, gain Na+, and stop growing. A marked increase in the rate of K+ efflux accounts for this loss; Na+, K+-ATPase pump activity increases but does not fully compensate for enhanced K+ efflux. Phorbol esters and cycloheximide inhibit K+ loss in Ca2+-deficient medium. Phorbol esters inhibit K+ efflux from human fibroblasts as well, even at physiological levels of Ca2+. Two cell lines derived from NIH-3T3, one transformed by a simian virus 40 deletion mutant, the other by the polyoma virus oncogene encoding the middle-sized tumor antigen, retain K+ and …

Determination Of Antibody Levels In Periapical Lesions Against Sixteen Oral Microorganisms By The Elisa Technique, Sammee Lee Jones

Loma Linda University Electronic Theses, Dissertations & Projects

The root canal system, which includes the periapical region of the tooth, may become infected by several types of microorganisms which are normally associated with these tissues. A bacteria-specific antibody response may occur at the local level, where various classes of immunoglobulins have been found and reported in the literature. The purpose of this study was to quantitatively measure locally produced immunoglobulin' levels in periapical lesion abscess materials and to determine the specific reactivity of these antibodies to several microorganisms which are normal flora, as well as being previously associated with the development and pathogenesis of endodontic infections. Periapical lesions …

Policy For Identifying And Assessing The Health Risks Of Toxic Substances, Maine Bureau Of Health

Maine Collection

Policy for Identifying and Assessing the Health Risks of Toxic Substances

by Norman T. Anderson, Environmental Toxicology Program, Division of Disease Control, Bureau of Health (February, 1988).

Contents: Section I: Introduction / Section II: Methods / Section III: Exposure Assessment / Section IV: Hazard Identification / Section V: Hazard Assessment / Section VI: Risk Characterization / List of Figures / List of Tables

Neurokinin Regulation Of Midbrain Raphe Neurons: A Behavioral And Anatomical Study, Joseph Paris

Dissertations

No abstract provided.

Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon

School of Veterinary and Biomedical Sciences: Faculty Publications

An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 ,ug of spectinomycin per ml (TSA-S400), TSA-S400 with 25 ,ug each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers …

1988 Winter No. 15, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 15 Winter 1988 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

1988 Winter No. 18, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 18 Winter1988-89 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 28 pages.

Oru Chapel March 18, 1987, Oral Roberts

City of Faith

This is a transcript of an ORU Chapel held on March 18, 1987 on the campus of Oral Roberts University. In this Chapel, President Roberts explains the need for $8 Million to keep the City of Faith, the Medical Missions Program, and Oral Roberts University afloat. As an act of faith, Roberts tells the students he will remain in the ORU Prayer Tower until April 1st, the deadline for raising the funds.

Kinetics Of Na/Li Exchange In High-K (Hk) And Low-K (Lk) Sheep Red Cells (Src), K. H. Ryu, Norma C. Adragna, D. Bradley, Peter K. Lauf

Pharmacology and Toxicology Faculty Publications

No abstract provided.

Comparison Of Virulent And Avirulent Legionella Pneumophila And Evaluation Of Fish As A Potential Environmental Reservoir/Experimental Model, Sandra Reading Sommer

Theses and Dissertations

Legionella pneumophila was first recognized as a cause of human pneumonia in 1976 . Since then, much has been learned about the microbiology, pathophysiology and epidemiology of this organism. The features which permit one strain but not another to invade human lung tissue and produce disease remain incompletely understood. This study e valuated several attributes of a virulent and an avirulent strain of L. pneumophila in an attempt to identify characteristics which would distinguish the two. Evaluation of a new medium, buffered egg yolk agar, showed that virulence was maintained after 26 passages, which was the same as the buffered …

Variant Forms Of Ataxia Telangiectasia, A. M. R. Taylor, E. Flude, B. Laher, Michael W. Stacey, E. Mckay, J. Watt, S. H. Greens, A. E. Harding

Bioelectrics Publications

Two ataxia telangiectasia patients with unusual clinical and cellular features are described. Cultured fibroblasts and PHA stimulated lymphocytes from these two patients showed a smaller increase of radiosensitivity than cells from other A-T patients, as measured by colony forming ability or induced chromosome damage respectively, after exposure to ionising radiation. The response of DNA synthesis to irradiation of these cells was, however, the same as for other A-T patients. Cells from a third patient with some clinical features of A-T but with a very protracted course also showed low levels of radiation induced chromosome damage, but colony forming ability and …

Healing Outreach Center Pamphlet, Holy Spirit Research Center, Oral Roberts University

City of Faith

This is c. 1987 brochure is a four page advertisement announcing phase II of the Healing Outreach Center and including Journey Through the Bible Center on the campus of Oral Roberts University. The Journey Through the Bible was an interactive media presentation of biblical stories in decorated rooms. Phase one contained the creation room and Noah's ark. This pamphlet promoted Phase II which was being planned.

Nosocomial Respiratory Tract Infections Associated With The Use Of Ventilatory Support Systems: Epidemiological And Bacteriological Study Of The Effect Of Changing Breathing Circuits At 24 Or 48 Hours, Virginia Archer Lamb

Theses and Dissertations

Nosocomial (hospital-acquired) pneumonia (HAP) continues to be an important cause of morbidity and mortality in the hospital. HAP is the third most common nosocomial infection after urinary tract and surgical wound infections. In addition, HAP is the nosocomial infection with the highest mortality rate. These infections are often difficult to treat, because most are caused by Gram-negative bacilli (GNB) that may be highly resistant to antimicrobial agents. HAPs frequently occur in intensive care patients with underlying lung and/or systemic diseases. Many patients are intubated and are on assisted ventilation. Several sources of infection associated with ventilators or respirators have been …

Tyrosyl Kinase Activity Is Inversely Related To Prostatic Acid Phosphatase Activity In Two Human Prostate Carcinoma Cell Lines., Ming-Fong Lin, Ching-Li Lee, Gail M. Clinton

Journal Articles: Biochemistry & Molecular Biology

Alterations in prostatic acid phosphatase (PAcP), a phosphotyrosyl phosphatase, corresponded to changes in overall tyrosyl kinase activity. PAcP added to extracts of prostate carcinoma cells with a low endogenous level of PAcP activity and elevated tyrosyl kinase activity decreased the tyrosyl kinase activity. On the other hand, when PAcP activity was decreased by the addition of androgens to cells, there was a corresponding increase in tyrosyl kinase activity.

Role Of Serum Amyloid P Component In Immune Clearance, Cindy L. Bristow

MUSC Theses and Dissertations

In order to clarify the mechanism of interaction of serum amyloid P component (SAP) with complement, the interaction of SAP with Clq was studied. It is known that SAP binds Sepharose 4B in the presence of calcium. 125I-Clq was retained on the Sepharose when purified 125I-Clq was incubated with SAP prior to affinity chromatography on Sepharose. In the absence of SAP, the 125I-Clq was not retained. To further examine the interaction of SAP with Clq, SAP was incubated at varying ratios with Clq. These mixtures were examined via crossed immunoelectro-immunoelectrophoresis against goat anti-SAP. A change in the electrophoretic behavior of …

Electrophysiology Of Flounder Intestinal Mucosa. I. Conductance Properties Ofthe Cellular And Paracellular Pathways, Dan R. Halm, Edward J. Krasny Jr., Raymond A. Frizzell

Neuroscience, Cell Biology & Physiology Faculty Publications

We evaluated the conductances for ion flow across the cellular and paracellular pathways of flounder intestine using microelectrode techniques and ion-replacement studies. Apical membrane conductance properties are dominated by the presence of Ba-sensitive K channels. An elevated mucosal solution K concentration, [K]_m, depolarized the apical membrane potential (ψ_a) and, at [K]_m less than 40 mM, the K dependence of ψ_a was abolished by 1-2 mM mucosal Ba. The basolateral membrane displayed Cl conductance behavior, as evidenced by depolarization of the basolateral membrane potential (ψ_b) with reduced serosal Cl concentrations, [Cl]_s. …

Electrophysiology Of Flounder Intestinal Mucosa. Ii. Relation Of The Electrical Potential Profile To Coupled Nacl Absorption, Dan R. Halm, Edward J. Krasny Jr., Raymond A. Frizzell

Neuroscience, Cell Biology & Physiology Faculty Publications

We characterized the hyperpolarization of the electrical potential profile of flounder intestinal cells that accompanies inhibition of NaCl cotransport. Several observations indicate that hyperpolarization of ψ_a and ψ_b (Δψ_a,b) results from inhibition of NaCl entry across the apical membrane: (a) the response was elicited by replacement of mucosal solution Cl or Na by nontransported ions, and (b) mucosal bumetanide or serosal cGMP, inhibitors of NaCl influx, elicited Δψ_a,b and decreased the transepithelial potential (ψ_t) in parallel. Regardless of initial values, ψ_a and ψ_b approached the equilibrium potential for K (E^{K …}

1985 Spring No. 7, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 7 Spring 1985 was edited by Russell Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

Interleukin 2 Regulates Its Own Receptors., Kendall A. Smith, Doreen A. Cantrell

Dartmouth Scholarship

The cell surface density of high-affinity membrane receptors for the T-lymphocytotrophic hormone interleukin 2 (IL-2) determines the rate of T-cell-cycle progression. Since 10-fold greater numbers of IL-2 receptor molecules were found by using a radiolabeled monoclonal antibody reactive with IL-2 receptors (anti-Tac) compared with binding of IL-2, the functional relationship of the binding sites recognized by both of these ligands was assessed. In the presence of cycloheximide, IL-2 binding sites declined with a half-time (t1/2) of 2.6 hr, whereas the decay of anti-Tac binding sites was much slower (t 1/2 = 6.4 hr). Moreover, after limited membrane proteolysis, the half-time …

Extrathyroidal Human Biochemistry Of Monoiodotyrosine, Stanley Andrew Tan

Loma Linda University Electronic Theses, Dissertations & Projects

The existence of extrathyroidal monoiodotyrosine (MIT) has been debated because of uncertain results from measuring serum MIT. The reason is that the isotopic and chromatographic assays used have not been sensitive enough. This investigation undertook to establish the existence of MIT in serum utilizing a radioimmunoassay (RIA), to study the physiology of serum MIT, and to discover the pharmacologic effects of exogenous MIT.

MIT-binding antibody was raised in rabbits by immunization with an MIT-porcine thyroglobulin conjugate. The antiserum bound 40% of the labeled MIT at a final dilution of 1:1000. Incubation of 100 µ1 antiserum with 50 pg [^{125 …}

Lifeline - City Of Faith Newsletter (May-June, 1984), Holy Spirit Research Center Oru Library

City of Faith

Lifeline vol 2, no 3 (May-June - 1984)

Lifeline is the newsletter for the employees and staff of the City of Faith Medical and Research Center. This edition includes the following articles:

• "Quick Care Meeting South Tulsans' Needs"
• "Research at the City of Faith: The Quest for a Breakthrough"
• "Diabetes Institute Tests 'Miracle' Drug"
• "Grants Provide Research in Number of Areas"
• "Alzheimer's Disease Research Fund Established"
• "Optical Clinic Focuses on Quality Eye Care"
• "The Case for City of Faith Marketing"
• "Miracles Expected in Informations Systems"
• "COF Communications Reaches Out and Touches Everyone"
• "Welcome New Employees and Staff"

Triggering Of The T3-Ti Antigen-Receptor Complex Results In Clonal T-Cell Proliferation Through An Interleukin 2-Dependent Autocrine Pathway., Stefan C. Meuer, Rebecca E. Hussey, Doreen A. Cantrell, James C. Hodgdon, Stuart F. Schlossman, Kendall A. Smith, Ellis L. Reinherz

Dartmouth Scholarship

Human T-cell clones and anti-T-cell-receptor antibodies (clonotypic) directed at surface receptors for antigen (T3-Ti molecular complex) as well as anti-interleukin 2 (IL-2) and anti-IL-2-receptor antibodies were utilized to investigate the mechanism by which alloantigens or antigen plus self-major histocompatibility complex (MHC) (i.e., physiologic ligand) trigger specific clonal proliferation. Soluble or Sepharose-bound anti-Ti monoclonal antibodies, like physiologic ligand, enhanced proliferative responses to purified IL-2 by inducing a 6-fold increase in surface IL-2 receptor expression. In contrast, only Sepharose-bound anti-Ti or physiologic ligand triggered endogenous clonal IL-2 production and resulted in subsequent proliferation. The latter was blocked by antibodies directed at either …

Interferon Gamma Blocks The Growth Of Toxoplasma Gondii In Human Fibroblasts By Inducing The Host Cells To Degrade Tryptophan., E. R. Pfefferkorn

Dartmouth Scholarship

Treatment of human fibroblasts with human recombinant gamma interferon blocked the growth of Toxoplasma gondii, an obligate intracellular protozoan parasite. Growth of the parasite was measured by a plaque assay 7 days after infection or by the incorporation of [3H]uracil 1 or 2 days after infection. The antitoxoplasma activity induced in the host cells by gamma interferon was strongly dependent upon the tryptophan concentration of the medium. Progressively higher minimal inhibitory concentrations of gamma interferon were observed as the tryptophan concentration in the culture medium was increased. Treatment with gamma interferon did not make the cells impermeable to tryptophan. The …

Effect Of Membrane Filtration Of Antimalarial Drug Solutions On In Vitro Activity Against Plasmodium Falciparum, J. Kevin Baird, C. Lambros

United States Public Health Resources

Antimalarial activities ofchloroquine, mefloquine, amodiaquine, and quinine in vitro against Plasmodium falciparum were diminished as a consequence ofmembranefiltration. Filtered drug solutions gave ID₅₀ values up to 25-fold greater than those of non-filtered (ethanol-sterilized) drug solutions. Loss ofactivity byfiltration was overcome by increasing the drug concentration prior to filtration. Water solutions filtered through Millex-GSfilter units consistently showed an absorbance maximum at 277 nm, accompanied by a lesser peak at 225 nm. Water filtrates from Nucleopore and Millex-G Vfilters showed no absorbance at 277 nm and only slight absorbance was evidentfor the Gelmanfilter unit. Activity losses were attributed to extractable contaminating …

The Effect Of Visible Light On Carotenogenesis Of Arthrosporulating Trichophyton Mentagrophytes, Ruth C. Mock

Dissertations

No abstract provided.

Activation Of B Lymphocytes By Monovalent Anti-Lyb-2 Antibodies, Subbarao Bondada, D. E. Mosier

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Small resting B lymphocytes are activated when their cell surface immuno-globulin (Ig) receptors interact with specific antigen or anti-Ig antibodies. Such a receptor-ligand interaction leads to enlargement and proliferation of B lymphocytes. These activated B cells can subsequently mature into Ig-secreting cells in the presence of appropriate accessory cell signals (1-3). However, monovalent anti-Ig antibodies cannot induce B cell activation even though they can bind to the surface Ig (4-7). It has thus been concluded that membrane Ig has to be cross-linked to induce early steps in B cell activation (membrane depolarization; 8) as well as later steps (proliferation). T …