Digital Commons Network^™

Thiazolyl N-Benzyl-Substituted Acetamide Derivatives: Synthesis, Src Kinase Inhibitory And Anticancer Activities, Asal Fallah-Tafti, Alireza Foroumadi, Rakesh Tiwari, Amir Nasrolahi Shirazi, David G. Hangauer, Yahao Bu, Tahmineh Akbarzadeh, Keykavous Parang, Abbas Shafiee

Pharmacy Faculty Articles and Research

KX2-391 (KX-01/Kinex Pharmaceuticals), N-benzyl-2-(5-(4-(2-morpholinoethoxy)phenyl)pyridin-2-yl)acetamide, is a highly selective Src substrate binding site inhibitor. To understand better the role of pyridine ring and N-benzylsubstitution in KX2-391 and establish the structure-activity relationship, a number of N-benzyl substituted (2-morpholinoethoxy)phenyl)thiazol-4-yl)acetamide derivatives containing thiazole instead of pyridine were synthesized and evaluated for Src kinase inhibitory activities. The unsubstituted N-benzyl derivative (8a) showed the inhibition of c-Src kinase with GI50 values of 1.34 μM and 2.30 M in NIH3T3/c-Src527F and SYF/c-Src527F cells, respectively. All the synthesized compounds were evaluated for inhibition of cell proliferation of human colon carcinoma (HT-29), breast carcinoma (BT-20), and leukemia (CCRF-CEM) cells. …

Targeted Identification Of Metastasis-Associated Cell-Surface Sialoglycoproteins In Prostate Cancer, Lifang Yang, Julius O. Nyalwidhe, Sigi Guo, Richard R. Drake, O. John Semmes

Bioelectrics Publications

Covalent attachment of carbohydrates to proteins is one of the most common post-translational modifications. At the cell surface, sugar moieties of glycoproteins contribute to molecular recognition events involved in cancer metastasis. We have combined glycan metabolic labeling with mass spectrometry analysis to identify and characterize metastasis-associated cell surface sialoglycoproteins. Our model system used syngeneic prostate cancer cell lines derived from PC3 (N2, nonmetastatic, and ML2, highly metastatic). The metabolic incorporation of AC₄ManNAz and subsequent specific labeling of cell surface sialylation was confirmed by flow cytometry and confocal microscopy. Affinity isolation of the modified sialic-acid containing cell surface proteins …

Roles Of The 15-Kda Selenoprotein (Sep15) In Redox Homeostasis And Cataract Development Revealed By The Analysis Of Sep 15 Knockout Mice, Marina V. Kasaikina, Dmitri E. Fomenko, Vyacheslav M. Labunskyy, Salil A. Lachke, Wenya Qiu, Juliet A. Moncaster, Jie Zhang, Mark W. Wojnarowicz, Sathish Kumar Natarajan, Mikalai I. Malinouski, Ulrich Schweizer, Petra A. Tsuji, Bradley A. Carlson, Richard L. Maas, Marjorie F. Lou, Lee E. Goldstein, Dolph L. Hatfield, Vadim N. Gladyshev

School of Veterinary and Biomedical Sciences: Faculty Publications

The 15-kDa selenoprotein (Sep15) is a thioredoxin-like, endoplasmic reticulum-resident protein involved in the quality control of glycoprotein folding through its interaction with UDP-glucose:glycoprotein glucosyltransferase. Expression of Sep15 is regulated by dietary selenium and the unfolded protein response, but its specific function is not known. In this study, we developed and characterized Sep15 KO mice by targeted removal of exon 2 of the Sep15 gene coding for the cysteinerich UDP-glucose:glycoprotein glucosyltransferase-binding domain. These KO mice synthesized a mutant mRNA, but the shortened protein product could be detected neither in tissues nor in Sep15 KO embryonic fibroblasts. Sep15 KO mice were viable …

Analysis Of Gaba And Glutamate In The Mouse Dorsal Striatum, Stella Wisidagamage

Wayne State University Theses

ABSTRACT

ANALYSIS OF GABA AND GLUTAMATE IN MICE BRAIN

by

STELLA WISIDAGAMA

May 2011

Advisor: Dr. Tiffany Mathews

Major: Chemistry (Analytical)

Degree: Master of Science

GABA and glutamate are the predominant inhibitory and excitatory amino acid neurotransmitters in the central nervous system (CNS), respectively. However, their altered levels cause several neurological diseases including Parkinson's and Huntington's disease, schizophrenia, and epilepsy. It is important to measure their levels in the extra- and intracellular environments to better understand and develop improved therapeutics that will treat these neurological disorders. The principle aim of this study is to study the impacts of different endogenous …

The Short Term Effect Of The Law Prohibiting Smoking In Enclosed Areas Upon The Smoking Conditions Of Employees In Kayseri, Turkey, Ahmet Öztürk, Serpi̇l Poyrazoğlu, Şule Şarli

Turkish Journal of Medical Sciences

A law prohibiting smoking in the enclosed areas of public and private workplaces was put into effect in Turkey on 19 May 2008. The objective of this study was to evaluate the effect of this law upon the smoking habits of the employees in this short period of 3 months. Materials and methods: This study was performed in Kayseri, in 3 public and 3 private workplaces, comprising 868 employees. A questionnaire was filled out, face-to-face, in the second week of August. Results: The ratio of the people supporting this smoke-free law was 68.7%. Among the employees who were smoking when …

The Extent Of Blockade Following Axillary, Supraclavicular, And Interscalene Approaches Of Brachial Plexus Block, Demet Coşkun, Ahmet Mahli̇

Turkish Journal of Medical Sciences

To investigate the onset, quality, and extent of the sensory and motor blocks in brachial plexus blocks performed through axillary, supraclavicular, or interscalene approaches. Materials and methods: This study involved 75 patients scheduled for orthopedic surgery of the upper extremity. Brachial plexus block was performed in patients through axillary (group AX, n = 25), supraclavicular (group SC, n = 25), or interscalene (group IS, n = 25) approaches. Results: Excluding intercostobrachial nerve, the adequate sensory and motor block rates in group AX on the nerves of brachial plexus were found to be 100% and 92%-100%, respectively. Sensory and motor block …

Retrospective Evaluation Of Laboratory-Confirmed And Recovered Cases Of Influenza A(H1n1)V, Funda Sevencan, Mehmet Meli̇kşah Ertem, Neval Özçullu, Vedat Dorman, Abdulvahi̇t Ormanli, Namik Kemal Kubat, Nurhan Albayrak, Ayşe Başak Altaş

Turkish Journal of Medical Sciences

To retrospectively evaluate patients that were diagnosed with H1N1 and recovered from the disease in the initial period of an influenza A(H1N1)v epidemic in the province of Diyarbakir. It is estimated that 34-67 million individuals around the world have been infected by influenza A(H1N1)v, that 154,000-303,000 individuals have been hospitalized, and that 7000-13,000 individuals have lost their lives. Materials and methods: We were able to include 250 of 362 cases (69.0%) of confirmed influenza A(H1N1)v, from which the patients recovered, from the samples received between 19 October and 3 December 2009 after a pandemic alarm was given in the province …

Ventricular Functions, Aortic Elastic Properties, And Endothelial Functions In Patients With Hypertensive Response To Treadmill Exercise Testing, Ri̇fat Eralp Ulusoy, Mehmet Yokuşoğlu, Oben Baysan, Ata Kirilmaz, Fethi̇ Kiliçaslan, Beki̇r Sitki Cebeci̇

Turkish Journal of Medical Sciences

Hypertensive response to treadmill exercise testing (TET) is an early marker of future development of hypertension. In this cross-sectional, case-control study we evaluated the relationship of hypertensive response to TET with aortic elastic properties, endothelial functions, and left ventricular functions. Materials and methods: The study group comprised 33 individuals who underwent TET with suspicion of myocardial ischemia, but no evidence of ischemia and with hypertensive response to TET, and the control group comprised 29 age- and gender-matched subjects. All participants underwent echocardiographic examination, brachial artery ultrasonography, and aortic ultrasonography for the assessment of left ventricular functions, endothelial functions by determining …

Some Ethical Reflections On Weight-Loss Diets, Hakan Erti̇n, Bülent Özaltay

Turkish Journal of Medical Sciences

To assess how weight-loss diets tend to move away from the authority of conventional medicine and become a field of abuse. Endocrinologists and specialists on metabolic diseases are rarely called upon to speak about obesity, whereas physicians from unrelated fields or nonphysicians frequently appear in the media. Materials and methods: The literature related with the field was reviewed and interpreted from an ethical perspective. Results: It is presented medically doubtful solutions via television and the internet. Several weight-loss drugs are introduced to the market without adequate controls and then have to be withdrawn after having caused serious damage to the …

Plasma Ldl Subtype Distribution In Patients With Or Without Coronary Stenosis, Yeşi̇m Er Öztaş, Çağdaş Özdöl, Levent Karaca

Turkish Journal of Medical Sciences

Plasma low density lipoprotein (LDL) had 3 subtypes according to its separation by polyacrylamide gradient gel electrophoresis. Of these, the small, dense (Sd) LDL particles have been accepted as an emerging cardiovascular risk factor. This study was conducted to assess the LDL subtypes in a group of patients undergoing coronary angiography. Materials and methods: The study involved 56 patients—36 of whom had at least 1 vessel stenosed (stenosis group)—and 20 patients who had no stenosis (non-stenosis group). LDL subtypes were determined according to their migration pattern after non-denaturing gradient gel electrophoresis. Total cholesterol, LDL and high density lipoprotein (HDL), and …

Risk Factors For Smoking Behavior Among University Students, Sevgi̇ Yurt Öncel, Ömer Lütfi̇ Gebi̇zli̇oğlu, Fazi̇l Ali̇ev Ali̇oğlu

Turkish Journal of Medical Sciences

To identify factors associated with increased smoking risks among Kırıkkale University students using a questionnaire. Smoking is a widespread habit in Turkey and a major public health problem in the world. Materials and methods: We assessed 1734 (11.6% of 15,000 total) students (869 males and 866 females, both smokers and nonsmokers) at Kırıkkale University with the questionnaire, which included questions about age, gender, smoking status of student, smoking status and education levels of parents, income, daily sports activities, smoking history (age when started or quit smoking, daily average number of cigarettes smoked, attempts to quit smoking, the reasons for starting …

Tca Cycle Inactivation In Staphylococcus Aureus Alters Nitric Oxide Production In Raw 264.7 Cells, Chandirasegaran Massilamany, Arunakumar Gangaplara, Donald J. Gardner, James M. Musser, David J. Steffen, Greg A. Somerville, Jay Reddy

Jay Reddy Publications

Inactivation of the Staphylococcus aureus tricarboxylic acid (TCA) cycle delays the resolution of cutaneous ulcers in a mouse soft tissue infection model. In this study, it was observed that cutaneous lesions in mice infected with wild-type or isogenic aconitase mutant S. aureus strains contained comparable inflammatory infiltrates, suggesting the delayed resolution was independent of the recruitment of immune cells. These observations led us to hypothesize that staphylococcal metabolism can modulate the host immune response. Using an in vitro model system involving RAW 264.7 cells, the authors observed that cells cultured with S. aureus aconitase mutant strains produced significantly lower amounts …

Rhodococcus Equi Venous Catheter Infection: A Case Report And Review Of The Literature, Rosalinda Guerrero, Ashish Bhargava, Zeina Nahleh

Wayne State University Associated BioMed Central Scholarship

Abstract

Introduction

Rhodococcus equi is an animal pathogen that was initially isolated from horses and is being increasingly reported as a cause of infection in humans with impaired cellular immunity. However, this pathogen is underestimated as a challenging antagonist and is frequently considered to be a mere contaminant despite the potential for life-threatening infections. Most case reports have occurred in immunocompromised patients who have received organ transplants (for example kidney, heart, bone marrow) or those with human immunodeficiency virus infection. Infections often manifest as pulmonary involvement or soft tissue abscesses. Bacteremia related to R. equi infections of tunneled central venous …

Rad6b Acts Downstream Of Wnt Signaling To Stabilize Β-Catenin: Implications For A Novel Wnt/Β-Catenin Target, Brigitte Gerard, Larry Tait, Pratima Nangia-Makker, Malathy Pv Shekhar

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Aberrant Wnt/β-catenin signaling is associated with breast cancer even though genetic mutations in Wnt signaling components are rare. We have previously demonstrated that Rad6B, an ubiquitin conjugating enzyme, stabilizes β-catenin via polyubiqutin modifications that render β-catenin insensitive to proteasomal degradation. Rad6B is a transcriptional target of β-catenin, creating a positive feedback loop between Rad6B expression and β-catenin activation.

Methods

To isolate subpopulations expressing high or low Rad6B levels, we transfected MDA-MB-231 or WS-15 human breast cancer cells with ZsGreen fluorescent reporter vector in which the expression of ZsGreen was placed under the control of Rad6B promoter. ZsGreen^{high …}

Histone Deacetylases (Hdacs) In Xpc Gene Silencing And Bladder Cancer, Xiaoxin S. Xu, Le Wang, Judith Abrams, Gan Wang

Wayne State University Associated BioMed Central Scholarship

Abstract

Bladder cancer is one of the most common malignancies and causes hundreds of thousands of deaths worldwide each year. Bladder cancer is strongly associated with exposure to environmental carcinogens. It is believed that DNA damage generated by environmental carcinogens and their metabolites causes development of bladder cancer. Nucleotide excision repair (NER) is the major DNA repair pathway for repairing bulk DNA damage generated by most environmental carcinogens, and XPC is a DNA damage recognition protein required for initiation of the NER process. Recent studies demonstrate reduced levels of XPC protein in tumors for a majority of bladder cancer patients. …

Analysis Of Positional Candidate Genes In The Aaa1 Susceptibility Locus For Abdominal Aortic Aneurysms On Chromosome 19, John H. Lillvis, Yoshiki Kyo, Gerard Tromp, Guy M. Lenk, Ming Li, Qing Lu, Robert P. Igo Jr, Natzi Sakalihasan, Robert E. Ferrell, Charles M. Schworer, Zoran Gatalica, Susan Land, Helena Kuivaniemi

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Abdominal aortic aneurysm (AAA) is a complex disorder with multiple genetic risk factors. Using affected relative pair linkage analysis, we previously identified an AAA susceptibility locus on chromosome 19q13. This locus has been designated as the AAA1 susceptibility locus in the Online Mendelian Inheritance in Man (OMIM) database.

Methods

Nine candidate genes were selected from the AAA1 locus based on their function, as well as mRNA expression levels in the aorta. A sample of 394 cases and 419 controls was genotyped for 41 SNPs located in or around the selected nine candidate genes using the Illumina GoldenGate platform. …

A Protein Network-Guided Screen For Cell Cycle Regulators In Drosophila, Stephen T. Guest, Jingkai Yu, Dongmei Liu, Julie A. Hines, Maria A. Kashat, Russell L. Finley Jr

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Large-scale RNAi-based screens are playing a critical role in defining sets of genes that regulate specific cellular processes. Numerous screens have been completed and in some cases more than one screen has examined the same cellular process, enabling a direct comparison of the genes identified in separate screens. Surprisingly, the overlap observed between the results of similar screens is low, suggesting that RNAi screens have relatively high levels of false positives, false negatives, or both.

Results

We re-examined genes that were identified in two previous RNAi-based cell cycle screens to identify potential false positives and false negatives. We …

A Retinoic Acid–Dependent Checkpoint In The Development Of Cd4+ T Cell–Mediated Immunity, Karina Pino-Lagos, Yanxia Guo, Chrysothemis Brown, Matthew P. Alexander, Raúl Elgueta, Kathryn A. Bennett, Victor De Vries, Elizabeth Nowak, Rune Blomhoff, Shanthini Sockanathan, Roshantha A. Chandraratna, Ethan Dmitrovsky, Randolph J. Noelle

Dartmouth Scholarship

It is known that vitamin A and its metabolite, retinoic acid (RA), are essential for host defense. However, the mechanisms for how RA controls inflammation are incompletely understood. The findings presented in this study show that RA signaling occurs concurrent with the development of inflammation. In models of vaccination and allogeneic graft rejection, whole body imaging reveals that RA signaling is temporally and spatially restricted to the site of inflammation. Conditional ablation of RA signaling in T cells significantly interferes with CD4⁺ T cell effector function, migration, and polarity. These findings provide a new perspective of the role of …

Stem Cell Biology And Strategies For Therapeutic Development In Degenerative Diseases And Cancer, Angel A. Alvarez

Electronic Theses and Dissertations

Stem cell biology is an exciting field that will lead to significant advancements in science and medicine. We hypothesize that inducing the expression of stem cell genes, using the embryonic stem cell gene nanog, will reprogram cells and dedifferentiate human mesenchymal stem cells into pluripotent stem cells capable of neural differentiation. The aims of initial studies are as follows: Aim 1: Demonstrate that forced expression of the embryonic stem cell gene nanog induces changes in human mesenchymal stem cells to an embryonic stem cell-like phenotype. Aim 2: Demonstrate that induced expression of nanog up-regulates the expression of multiple embryonic stem …

Distinct Domains Of Bax Are Involved In Mitochondrial Bioenergetics And Apoptosis, Ge Zhang

Electronic Theses and Dissertations

Apoptosis is essential for cellular homeostasis and is also a pathologic feature of various diseases. The balance between Bcl-2 family proteins determines whether a cell will live or die. Bax, a member of the BCL-2 family proteins, is a pro-apoptotic protein that exists in both a soluble, cytoplasmic form and a membrane-bound form. Upon apoptotic stimuli, Bax undergoes a conformational change and translocates to the mitochondria, initiating apoptotic events. However, little is known about whether Bax is involved in the regulation of mitochondrial function under non-apoptotic conditions, and how Bax binds to mitochondria to exert its activity. Here, we investigate …

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

Invasive Device-Associated Nosocomial Infections Of A Teaching Hospital In Turkey; Four Years’ Experience, Aslihan Candevi̇r, Behi̇ce Kurtaran, Fi̇li̇z Ki̇bar, Emre Karakoç, Hasan Sali̇h Zeki̇ Aksu, Yeşi̇m Taşova

Turkish Journal of Medical Sciences

To determine our setting's IDAI rates, infecting microorganisms, and their resistance patterns to achieve standardization and make comparisons among other Turkish and developed country hospitals all over the world. Materials and methods: The numbers of total patient days, ventilator days, central catheter days and, urinary catheter days in the ICUs were recorded and IDAI rates were calculated. Clinical specimens were obtained from patients, cultivated at appropriate culture media, and infecting microorganisms and resistance patterns were determined. Results: Totally 1450 invasive device-associated infection episodes were determined (16.4% of patients) with a rate of 21.12/1000 days. Ventilator associated pneumonia rate was 22.05/1000 …

Serum Osteoprotegerin (Opg) Measurement In Behçet's Disease, Nafi̇ye Fulya İlhan, Nesri̇n Demi̇r, Tamer Demi̇r, Ahmet Gödekmerdan

Turkish Journal of Medical Sciences

Recent studies indicate that osteoprotegerin (OPG) acts as an important molecule in the development of vascular diseases. The aim of the present study was to examine the association between serum OPG levels and Behcet's disease (BD). Materials and methods: Twenty-five patients with BD and 20 healthy control subjects, matched for age and sex, were included in this study. Serum levels of OPG were determined using ELISA. Results: We found that serum levels of OPG were significantly higher in patients with BD than in healthy control subjects (813.75 ± 11 vs. 655.56 ± 17 pg/mL, P = 0.002). There was no …

Aids Knowledge And Risky Sexual Behaviors Among Registered Female Sex Workers In Turkey, Tüli̇n Bedük, Hayri̇ye Ünlü, Veli̇ Duyan

Turkish Journal of Medical Sciences

To evaluate AIDS knowledge and risky sexual behaviors of registered female sex workers during their obligatory periodic examination in the Department of Dermatology and Sexually Transmitted Diseases, Ankara Metropolitan Municipality Hospital. Materials and methods: A questionnaire composed of items on socio-demographic features and knowledge levels on HIV/AIDS, its transmission routes, and risky sexual behaviors was administered via face to face interviews. Results: One hundred four sex workers were included in the study. Higher educated ones had more knowledge on AIDS, its transmission routes, and risky behaviors (P >= 0.05, P Keywords: Commercial sex workers, HIV/AIDS transmission, risky sexual behavior

An Investigation Of Genes Coding Fibronectin Binding Proteins In Staphylococcus Aureus Isolated From Carriers Aged Between 6 And 14 Years, Nur Yapar, Vi̇ldan Avkan Oğuz

Turkish Journal of Medical Sciences

Nasal carriage rates for Staphylococcus aureus have been reported to vary between 18% and 50% in different populations and it represents a risk factor for invasive infections. The aim of the present study was to investigate the presence of genes associated with fibronectin binding proteins mediating the adhesion of S. aureus to human epithelial cells. Materials and methods: Fifty strains isolated from nasal swab specimens of children were included. Specimens were inoculated on mannitol salt agar plates and after 24-48 h of incubation at 37 °C the isolates were identified according to their biochemical properties. The presence of fnbA and …

An Investigation Of The Antimicrobial Impact Of Drug Combinations Against Mycobacterium Tuberculosis Strains, Mi̇ne Hoşgör Li̇moncu, Şafak Ermertcan, Bayri Eraç, Hüseyi̇n Taşli

Turkish Journal of Medical Sciences

To investigate the in vitro activity of linezolid and ofloxacin in combination with first-line antituberculosis agents (isoniazid and rifampicin) against M. tuberculosis strains. The use of combinations that include fluoroquinolones and oxazolidinone is now being considered for the treatment of resistant Mycobacterium tuberculosis strains. Materials and methods: The minimum inhibitory concentrations of the test drugs were determined by the standard agar dilution method. The interaction of drug combinations was investigated by time-kill method. Results: A total of 9 M. tuberculosis strains were used in this study, 5 of which were multidrug-resistant (MDR) and 4 of which were not. The studied …

The Prenatal Diagnosis Of Familial Satellited Yq Chromosomes, Sezi̇n Yakut, Saffet Öztürk, Mehmet Şi̇mşek, İbrahi̇m İnanç Mendi̇lci̇oğlu, Güven Lüleci̇

Turkish Journal of Medical Sciences

To report a rare chromosomal abnormality, familial satellited Y chromosome (Yqs), in 3 Turkish prenatal cases. Material and methods: Metaphase chromosomes were prepared from amniocytes for prenatal cases and from lymphocytes for their fathers according to the standard methods. Results: In all cases, the analysis of all GTG-banded metaphases showed satellites on the long arms of chromosome Y as 46, XYqs karyotype. The indications for cytogenetic study were advanced maternal age and abnormal ultrasonographic findings with increased risk in the first trimester screening test. Cytogenetic studies of the fathers revealed that the Yqs were inherited. The satellites were positively stained …

Mechanisms And Consequences Of Thy-1 Shedding, John Edwin Bradley

All ETDs from UAB

Idiopathic Pulmonary Fibrosis (IPF) is characterized by chronic fibrosis in the lung interstitium of unknown etiology, and causes death within 2 to 4 years after diagnosis. There are no clinical interventions save for lung transplantation that give any survival benefit. Aberrant collagen deposition and aggregates of proliferating fibroblasts and myofibroblasts called fibroblastic foci (FF) are the classic features of IPF. Therefore, the cell type most often implicated as pathogenic in IPF is the fibroblast, especially its differentiated phenotype, the myofibroblast. Fibroblasts are a remarkably heterogeneous cell type. Expression of the cell surface glycoprotein Thy-1 (Thymocyte differentiation antigen 1) delineates a …

Sox2/Parylated Parp1 Complexes Regulate Pluripotency, Yi-Shin Lai

All ETDs from UAB

Embryonic stem cells (ESCs) were first derived from the inner cell mass (ICM) of blastocyst-stage embryos. ESCs are distinguished from other cell types by their pluripotency and unlimited self-replication. ESCs are able to differentiate into multiple cell types that represent all three primary germ layers (ectoderm, mesoderm and endoderm) and can form an entire adult organism. Under defined conditions, ESCs are self-renewable and have an extended life-span without compromising pluripotency. The plasticity of ESCs provides opportunities for regenerative medicine. The recent production of induced pluripotent stem cells (iPSC) offers an ideal "personalized" substitute for ESCs. Overexpression of four transcription factors, …

Limited Transplantation Of Antigen-Expressing Hematopoietic Stem Cells Induces Long-Lasting Cytotoxic T Cell Responses And Effect Of Altered Suppressive Myeloid Population On Hiv-Disease Progression, Warren L. Denning

All ETDs from UAB

While each type of cancer and chronic viral infection has its own specific pathology, they share two common mechanisms of immune evasion. The first mechanism is the exhaustion or deletion of antigen-specific T cells. The second mechanism is the formation of an immunosuppressive environment responsible for the block of T cell function. Elicitation of antigen-specific T cells be accomplished by immunotherapy in place of conventional treatments such as HAART and chemotherapy. In addition, immunotherapy can alleviate the side-effects associated with long-term use of conventional therapies while reducing the total cost. The results presented here provide an alternative to conventional methods …