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Over-Expression Of Mir-223 Induces M2 Macrophage Through Glycolysis Alteration And Attenuates Lipopolysaccharide-Induced Sepsis Mouse Model, A Proposal Sepsis Cell-Based Therapy, Dang Phi Cong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sepsis, a systemic infection with excessive inflammatory symptoms due to dysregulation of immune responses, remains the most concerned health-care problem worldwide with the mortality rate critically beyond the number of death cases in HIV, cancers or stroke. While it is well documented that pathogenesis of sepsis is intimately involved in dysregulation of macrophages, great effort has been made to discover efficacious therapy to control macrophage responses. However, macrophages are the most versatile immune cells with large spectrum of heterogeneous phenotypes and functions. Recently, collection of insights unravel the crucial role of metabolism as an orchestrator of immune regulation, intervention on …

การเปรียบเทียบองค์ประกอบของร่างกายในผู้ป่วยโรคอ้วนหลังเข้ารับการผ่าตัดกระเพาะเพื่อลดน้ำหนักกับองค์ประกอบของร่างกายในผู้ที่ไม่เคยได้รับการผ่าตัดกระเพาะเพื่อลดน้ำหนักมาก่อน, ศิรินรัตน์ ตั้งจิตตรง

Chulalongkorn University Theses and Dissertations (Chula ETD)

วัตถุประสงค์ เพื่อศึกษาการเปลี่ยนแปลงองค์ประกอบของร่างกายในผู้ป่วยโรคอ้วนหลังการผ่าตัดกระเพาะเพื่อลดน้ำหนัก ที่ระยะเวลา 12 เดือน และเปรียบเทียบกับกลุ่มผู้ที่ไม่เคยได้รับการผ่าตัดกระเพาะเพื่อลดน้ำหนักมาก่อน ที่มี อายุ เพศ และค่าดัชนีมวลกายใกล้เคียงกัน วิธีการวิจัย เป็นการวิจัยโดยการสังเกตเชิงวิเคราะห์โดยใช้ข้อมูลผู้ป่วยโรคอ้วนที่ได้รับการผ่าตัดกระเพาะเพื่อลดน้ำหนักด้วยวิธี Laparoscopic Roux-en-Y gastric bypass(LRYGB) หรือ Laparoscopic sleeve gastrectomy(LSG) ในโรงพยาบาลจุฬาลงกรณ์ ช่วงปี พ.ศ.2558-2562 ที่หลังผ่าตัดที่ 12 เดือน มีค่าดัชนีมวลกาย น้อยกว่า 30 กิโลกรัม/เมตร2 กลุ่มควบคุมคือกลุ่มผู้ที่ไม่เคยได้รับการผ่าตัดกระเพาะที่มี อายุ เพศ และค่าดัชนีมวลกายใกล้เคียงกัน และไม่มีโรคประจำตัว วิเคราะห์องค์ประกอบของร่างกายด้วยเครื่อง Inbody 770 ที่คลินิกอายุรกรรมโรคอ้วน โรงพยาบาลจุฬาลงกรณ์ ผลการศึกษา ผู้เข้าร่วมวิจัยทั้งหมด 60 คน แบ่งเป็นกลุ่มผู้ป่วยโรคอ้วนที่ได้รับการผ่าตัดกระเพาะเพื่อลดน้ำหนัก 30 คน กลุ่มควบคุม 30 คน จากการศึกษาพบว่าเมื่อเปรียบเทียบกับกลุ่มควบคุม กลุ่มผู้ป่วยมีค่าสัดส่วนเอวต่อสะโพก (waist-hip ratio)(0.83 และ 0.9, P-value <0.001) ร้อยละของไขมันในร่างกาย (percentage of body fat)(ร้อยละ 30.6 และ ร้อยละ 35.9, P-value 0.001) มวลไขมันบริเวณลำตัว (trunk fat mass)(10.3 กิโลกรัม และ 12.4 กิโลกรัม , P-value 0.04) และ มวลรวมกล้ามเนื้อและอวัยวะบริเวณแขนขา (appendicular lean mass)(9 กิโลกรัม และ 16.9 กิโลกรัม, P-value <0.001) น้อยกว่ากลุ่มควบคุม โดยมีค่ามวลรวมกล้ามเนื้อและอวัยวะทั้งร่างกาย (soft lean mass)(47.7 กิโลกรัม และ 39.9 กิโลกรัม, P-value 0.001) มวลรวมกล้ามเนื้อและอวัยวะบริเวณลำตัว (trunk lean mass)(21.2 กิโลกรัม และ 19 กิโลกรัม, P-value 0.02) มวลกล้ามเนื้อลาย (skeletal muscle mass)(27.5 กิโลกรัม และ 23 กิโลกรัม, P-value 0.003) และ มวลกายไร้ไขมัน (fat free mass)(51.1 กิโลกรัม และ 42.3 กิโลกรัม, P-value 0.001) มากกว่ากลุ่มควบคุมอย่างมีนัยสำคัญ และพบว่าหลังผ่าตัดกระเพาะเพื่อลดน้ำหนัก ที่ระยะเวลา 6 เดือน และ 12 เดือน ค่าตัวแปรองค์ประกอบของร่างกายลดลงทั้งหมด โดยเห็นผลลดลงมากสุดที่ระยะเวลา 12 เดือนหลังเข้ารับผ่าตัด สรุปการศึกษา แสดงให้เห็นว่า ในกลุ่มผู้ป่วยโรคอ้วนที่ได้รับการผ่าตัดกระเพาะเพื่อลดน้ำหนัก แม้ค่าตัวแปรองค์ประกอบของร่างกาย เช่น มวลไขมัน มวลกายไร้ไขมัน มวลกล้ามเนื้อ ลดลงทั้งหมดในช่วงตลอดระยะเวลา12 เดือน หลังเข้ารับการผ่าตัด แต่ มวลกล้ามเนื้อ และมวลกายไร้ไขมันในกลุ่มผู้ป่วยนั้น ยังคงสูงกว่าในกลุ่มควบคุมที่อายุ เพศ และค่าดัชนีมวลกายใกล้เคียงกัน

Classification Of Organoid Transcriptomic Profiles Unravelling Colorectal Cancer Molecular Subtypes, Pattarin Nuwongsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Colorectal cancer (CRC) is genetically and transcriptomically heterogeneous disease. Molecular subtyping of colorectal cancer using consensus molecular subtype (CMS) system demonstrated the potential predictive value for tumor progression and treatment response. However, the CMS system was developed from data of whole tissues containing both cancer and non-tumor transcripts components for classification which does not directly represent intrinsic heterogeneity of cancer cells. In this study genetic profiles of CRC organoids were investigated first, and the results indicate chromosomal instability (CIN) and microsatellite instability (MSI) as pathogenic pathways of CRC. Furthermore, the results also revealed diverse patterns of somatic mutations of these …

Modeling Genetic Risk Factor Of Alzheimer's Disease "Sorl1" Using Patient Specific Pluripotent Stem Cells, Kamonchanok Kongsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Alzheimer’s disease is the most common neurodegenerative; cause of dementia and trends to increase in elder society. There is still not cure. A new strategy development for treatment is needed. SORL1 is a major genetic risk factor associated with sporadic AD. It correlates with degrading amyloid beta (Aβ) by sorting to lysosome. SORL1 overexpression has been reported that reduce Aβ production. However, the molecular mechanism that regulate in neurons is still unclear. The purpose of this study is to generate an effective drug discovery platform for identifying molecules that can modulate SORL1 level in human neurons by using induced pluripotent …

The Enhanced Danger Of Physicians’ Off-Label Prescribing, Doriane Lambelet Coleman, Philip M. Rosoff

Faculty Scholarship

The COVID-19 pandemic represents a major challenge to both technologically advanced and resource-poor countries. There are currently no effective treatments for severe disease other than supportive care and advanced life support measures, including the use of mechanical ventilators. With the urgency and necessity bred from desperation, there have been many calls to utilize unproven therapies, such as hydroxychloroquine, for which little evidence of efficacy exists. We have previously argued that such off-label use, while legal, is problematic (and even dangerous) and have suggested several regulatory remedies that could protect patients and advance their interests while preserving the reasonable authority of …

Identification Of A Novel Single Amino Acid Substitution (V666g) Of Jak1 From A Patient With Acute Lymphoblastic Leukemia Impairs Jak3 Mediated Il-2 Signaling, Alice Hernandez Grant

Open Access Theses & Dissertations

The Janus kinase (JAK) family, notably JAK1, JAK2 and JAK3 are recognized as oncogenic drivers in high risk Acute Lymphoblastic Leukemia (ALL). The bulk of activating JAK mutations are thought to occur within functional hot-spots across Janus Homology (JH) domains. The most frequently mutated regions is the JH2 pseudo-kinase, which provides a negative regulatory role to the adjacent catalytically active JH1 kinase domain. Despite the prevalence of JAK activating mutations and a need for new therapeutic inhibitors, there is a lack of understanding in the allosteric regulation of JAK kinases. Here we sought to identify mutations involved in driving ALL …

Muscular And Physical Response To An Agility And Repeated Sprint Tests According To The Level Of Competition In Futsal Players, Jorge García-Unanue, José Luis Felipe, David Bishop, Enrique Colino, Esther Ubago-Guisado, Jorge López-Fernández, Enrique Hernando, Leonor Gallardo, Javier Sánchez-Sánchez

Research outputs 2014 to 2021

© Copyright © 2020 García-Unanue, Felipe, Bishop, Colino, Ubago-Guisado, López-Fernández, Hernando, Gallardo and Sánchez-Sánchez. The aim of this study was to evaluate the neuromuscular response to an agility and repeated sprint ability (RSA) test according to the level of competition in futsal players. A total of 33 players from two elite teams and one amateur team participated in the study. The participants completed an agility t-test, a 30 m-speed test, and a RSA test. A countermovement jump (CMJ) test and a tensiomyography test of the rectus femoris (RF) and biceps femoris (BF) of both legs were carried out before and …

Microbiotyping The Sinonasal Microbiome, Emily K Cope, Mohammad Javed Ali, Benjamin S Bleier, Claudio Callejas, Marjolein E Cornet, Richard G Douglas, Daniel Dutra, Christos Georgalas, Richard J Harvey, Peter H Hwang, Amber U Luong, Rodney J Schlosser, Pongsakorn Tantilipikorn, Marc A Tewfik, Sarah Vreugde, Peter-John Wormald, J Gregory Caporaso, Alkis J Psaltis

Faculty, Staff and Student Publications

This study offers a novel description of the sinonasal microbiome, through an unsupervised machine learning approach combining dimensionality reduction and clustering. We apply our method to the International Sinonasal Microbiome Study (ISMS) dataset of 410 sinus swab samples. We propose three main sinonasal “microbiotypes” or “states”: the first is Corynebacterium-dominated, the second is Staphylococcus-dominated, and the third dominated by the other core genera of the sinonasal microbiome (Streptococcus, Haemophilus, Moraxella, and Pseudomonas). The prevalence of the three microbiotypes studied did not differ between healthy and diseased sinuses, but differences in their distribution were evident based …

The Development Of Novel Apurinic/Aprymidinic Endonuclease/Redox-Factor 1 Inhibitors For The Treatment Of Human Melanoma, Bella Sharifi

Pharmaceutical Sciences (MS) Theses

Apurinic/apyrimidinic DNA repair endonuclease-1 (APE1), first recognized as an important DNA excision repair enzyme, is also known as Redox Factor-1 (Ref-1) involved in the activation of many nuclear transcription factors in both redox-dependent and independent manner. It has been well-documented that the overexpression of APE/Ref-1 contributes to the development of chemo-resistance and is associated with tumor progression in many human malignancies [1].

Our previous study in melanoma demonstrated that the development of novel inhibitors targeting the redox regulation domain of APE/Ref-1 is a promising strategy for melanoma treatment. To date, limited successes have been reported in developing novel …

Altered Central Hemodynamics In Individuals With Down Syndrome, Thessa Irena Maria Hilgenkamp, Elizabeth Cornellia Schroeder, Sang Ouk Wee, Georgios Grigoriadis, Alexander Jacob Rosenberg, Tracy Baynard, Bo Fernhall

Physical Therapy Faculty Research

Background: Individuals with Down Syndrome (DS) have autonomic dysfunction impacting regulation of heart rate, Blood Pressure (BP), and peripheral vasoconstriction. This may alter central hemodynamics through different wave reflections. We investigated central hemodynamics including wave reflection during rest and a sympathoexcitatory stimulus [Lower Body Negative Pressure (LBNP)] in individuals with DS and controls. Methods: Radial applanation tonometry was performed on participants with and without DS before and during 5-min LBNP stimulus of −20 mmHg. Waveforms were calibrated to mean and diastolic BP. Generalized transfer function was used to estimate aortic pressures [Systolic Blood Pressure (aSBP), Diastolic Blood Pressure (aDBP), mean …

Single-Nuclei Rna-Seq On Human Retinal Tissue Provides Improved Transcriptome Profiling, Akbar Shakoor, Yumei Li, Sangbae Kim, Albert Vitale, Ivana Kim, Denise Morgan, Shaoheng Liang, Nathaniel Wu, Ken Chen, Margaret M Deangelis, Rui Chen

Faculty, Staff and Students Publications

Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with distinct functions. Six samples from three healthy donors are profiled and high-quality RNA-seq data is obtained for 5873 single nuclei. All major retinal cell types are observed and marker genes for each cell type are identified. The gene expression of the macular and peripheral retina is compared to each other at cell-type level. Furthermore, our dataset shows an …

Electrochemical Hydrogen Evolution Over Hydrothermally Synthesized Re-Doped Mos2 Flower-Like Microspheres, Luis Ballesteros, Julio Urzúa, Mario Farías, Francisco Paraguay-Delgado, Gabriel Alonso-Núñez, Guillermo González, Eglantina Benavente

Faculty, Staff and Students Publications

In this research, we report a simple hydrothermal synthesis to prepare rhenium (Re)- doped MoS2 flower-like microspheres and the tuning of their structural, electronic, and electrocatalytic properties by modulating the insertion of Re. The obtained compounds were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). Structural, morphological, and chemical analyses confirmed the synthesis of poorly crystalline Re-doped MoS2 flower-like microspheres composed of few stacked layers. They exhibit enhanced hydrogen evolution reaction (HER) performance with low overpotential of 210 mV at current density of 10 mA/cm2, with a …

Week Of December 16, 2019, New York Medical College

InTouch

• General Dental Practice Residents and M.P.H. Candidates Complete Federal Service Internship
• Department of Public Health Hosts Annual Student Appreciation Night

Trade-Offs Between Survival And Reproduction In Starvation-Selected Drosophila Melanogaster, Tammara Beeghly

UNLV Theses, Dissertations, Professional Papers, and Capstones

Evolutionary outcomes are demonstrated by traits that affect an organism’s schedule of reproduction and survival. These life history traits are reflected in an organism’s physiology, development and behavior. Environmental changes, such as availability of nutritional resources, can profoundly affect evolutionary outcomes of individuals and populations. When shortages arise, there will be trade-offs in the allocation of resources, where one trait prevails at the expense of another.

In the laboratory, we can mimic conditions in nature and study the specific effects of the conditions that we re-create. In our case, over 100 generations of Drosophila melanogaster have been selected for starvation …

Multiple Mitochondrial Thioesterases Have Distinct Tissue And Substrate Specificity And Coa Regulation, Suggesting Unique Functional Roles., Carmen Bekeova, Lauren Anderson-Pullinger, Kevin Boye, Felix Boos, Yana Sharpadskaya, Johannes M Herrmann, Erin L. Seifert

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Acyl-CoA thioesterases (Acots) hydrolyze fatty acyl-CoA esters. Acots in the mitochondrial matrix are poised to mitigate β-oxidation overload and maintain CoA availability. Several Acots associate with mitochondria, but whether they all localize to the matrix, are redundant, or have different roles is unresolved. Here, we compared the suborganellar localization, activity, expression, and regulation among mitochondrial Acots (Acot2, -7, -9, and -13) in mitochondria from multiple mouse tissues and from a model of Acot2 depletion. Acot7, -9, and -13 localized to the matrix, joining Acot2 that was previously shown to localize there. Mitochondria from heart, skeletal muscle, brown adipose tissue, and …

Week Of December 9, 2019, New York Medical College

InTouch

• HSL Opens New Reading Room
• Sixteenth Annual Louis R.M. DelGuercio Distinguished Visiting Professorship and Research Day Set For December 18
• Center for Disaster Medicine Brings Mass Casualty Triage Training to Western New York
• Support for Jewish Life Programming at NYMC and TouroCDM

3d Vessel-Wall Virtual Histology Of Whole-Body Perfused Mice Using A Novel Heavy Element Stain, P. Joy Dunmore-Buyze, Charmainne Cruje, Zengxuan Nong, Jason J. Lee, John A. Kiernan, J. Geoffrey Pickering, Maria Drangova

Medical Biophysics Publications

Virtual histology – utilizing high-resolution three-dimensional imaging – is becoming readily available. Micro-computed tomography (micro-CT) is widely available and is often coupled with x-ray attenuating histological stains that mark specific tissue components for 3D virtual histology. In this study we describe a new tri-element x-ray attenuating stain and perfusion protocol that provides micro-CT contrast of the entire vasculature of an intact mouse. The stain – derived from an established histology stain (Verhoeff’s) – is modified to enable perfusion through the vasculature; the attenuating elements of the stain are iodine, aluminum, and iron. After a 30-minute perfusion through the vasculature (10-minute …

Implementation Of Ventilator Bundle For Prevention Of Ventilator-Associated Pneumonia In Pediatric Intensive Care Unit, Abd-Elaleem Elgendy, Ahmed Y. Alsawah, Mahmoud M. Al-Shahat, Mohamed F. Alsoda, Mohammad A. M. Abboud, Sherif M. K. Reda

Journal of Medicine in Scientific Research

Background Ventilator-associated pneumonia (VAP) is associated with increased morbidity and mortality in pediatric intensive care unit (PICU) patients. Objective The aim was to examine the effect of adherence to VAP prevention bundle on the incidence of VAP in PICU. Patients and methods A prospective comparative study was conducted in Al-Hussein University Hospital on all patients admitted and ventilated in PICU in a year from September 2017 until September 2018. They were divided into two groups: the first group included patients admitted to PICU after implementation of the study, comprising 43 patients as cases, and the second group included patients admitted …

Cardiovascular Risk In Patients With Rheumatoid Arthritis: The Role Of Cholesterylester Transfer Protein, Ali M. Mursi, Emad M. Elshebiny, Lobna Y. Ebrahim, Sahar H. Quashwa, Sherry K. Abdelrahman, Taghreed F. Mohammed Mostafa

Journal of Medicine in Scientific Research

Aim To detect serum level of cholesterylester transfer protein (CETP) (the enzyme involved in reverse cholesterol transport) in patients with rheumatoid arthritis (RA) and to evaluate its relation to various clinical parameters of the disease, lipid profile, and carotid intima-media thickness (CIMT) as a marker of atherosclerosis and cardiovascular disease risk. Patients and methods This study involved a total of 80 participants, comprising 50 patients with RA and 30 age-matched and sex-matched healthy controls. Detailed medical history and thorough clinical examination (general and musculoskeletal) as well as laboratory investigations including lipid profile were performed for all patients with RA. Serum …

It's A Hard Nacht Life: Understanding How Nlrp12 Ticks, Abbigale Julia Brown

Graduate Theses/Dissertations

The protein NOD- like receptor pyrin domain containing 12 (NLRP12) comes from a family of protein receptors with a wide range of functions including fertility as well as anti-inflammatory properties. The biological role of NLRP12 is poorly understood: research on the mechanisms behind its function and/or activation remains contradictory between different cell models. Current research suggests its involvement in a multi-protein complex named the inflammasome. The alternative hypothesis that has also been proposed is that NLRP12 is not a part of the inflammasome, rather it negatively regulates a transcription factor known as NF-��B down stream of Toll-like receptors. NLRP12 is …

Msto1 Mutations Cause Mtdna Depletion, Manifesting As Muscular Dystrophy With Cerebellar Involvement., S Donkervoort, R Sabouny, P Yun, L Gauquelin, K R Chao, Y Hu, I Al Khatib, A Töpf, P Mohassel, B B Cummings, R Kaur, D Saade, S A Moore, L B Waddell, M A Farrar, J K Goodrich, P Uapinyoying, S H S Chan, A Javed, M E Leach, P Karachunski, J Dalton, L Medne, A Harper, C Thompson, Isabelle Thiffault, S Specht, R E Lamont, Carol J. Saunders, H Racher, F P Bernier, D Mowat, N Witting, J Vissing, R Hanson, Keith A. Coffman, Meagan K. Hainlen, J S Parboosingh, A Carnevale, G Yoon, R E Schnur, Care4rare Canada Consortium, K M Boycott, J K Mah, V Straub, A Reghan Foley, A M Innes, C G Bönnemann, T E Shutt

Manuscripts, Articles, Book Chapters and Other Papers

MSTO1 encodes a cytosolic mitochondrial fusion protein, misato homolog 1 or MSTO1. While the full genotype-phenotype spectrum remains to be explored, pathogenic variants in MSTO1 have recently been reported in a small number of patients presenting with a phenotype of cerebellar ataxia, congenital muscle involvement with histologic findings ranging from myopathic to dystrophic and pigmentary retinopathy. The proposed underlying pathogenic mechanism of MSTO1-related disease is suggestive of impaired mitochondrial fusion secondary to a loss of function of MSTO1. Disorders of mitochondrial fusion and fission have been shown to also lead to mitochondrial DNA (mtDNA) depletion, linking them to the mtDNA …

The Acute Effect Of Water Intake On Glucose Regulation In Low Drinkers, Adam David Seal

Graduate Theses and Dissertations

Previous evidence suggests the hormone arginine vasopressin (AVP) may be a modifiable factor contributing to hyperglycemia. Significant differences in urine concentration and copeptin, a marker for AVP, have been observed between low and high water drinkers. Purpose: The purpose was to investigate the acute effect of adequate water intake on glucose regulation in low drinkers. Methods: 7 healthy (5 males, 2 female) low drinkers were recruited using a water frequency questionnaire (WFQ), spot and 24-h urine sample (age 43±6 y, BMI 30.9±3, WFQ volume 823±403 mL∙d-1, 24 h Uosm 961±105 mmol∙kg-1, copeptin 8.17±3.05 pmol∙L-1). During two experimental protocols, participants remained …

Identification And Molecular Analysis Of Dna In Exosomes, Jena Tavormina

Dissertations and Theses (Open Access)

Exosomes are heterogeneous nanoparticles 50-150nm in diameter. Exosomes contain many functional cargo components, such as protein, DNA, and RNA. While protein and RNA exosome content has been extensively studied, very little work has been done to characterize exosomal DNA. Here, we demonstrate that exosomal DNA is heterogeneous and its packaging into exosomes is dependent on the cell of origin. Furthermore, through a rigorous assessment of various isolation methods, we identify Size Exclusion Chromatography (SEC) as the best method for the isolation of exosomal DNA for downstream applications. Additionally, we evaluate the methylation status of exosomal DNA and demonstrate that exosomal …

Structural Basis Of Amino Acid Surveillance By Higher-Order Trna-Mrna Interactions, Vassiliki Stamatopoulou, Nikoleta Giarimoglou, Frances E Henderson, Lixin Fan, Grigore D Pintilie, Kaiming Zhang, Muyuan Chen, Steven J Ludtke, Yun-Xing Wang, Constantinos Stathopoulos, Wah Chiu, Jinwei Zhang

Faculty, Staff and Students Publications

Amino acid availability in Gram-positive bacteria is monitored by T-box riboswitches. T-boxes directly bind tRNAs, assess their aminoacylation state, and regulate the transcription or translation of downstream genes to maintain nutritional homeostasis. Here, we report cocrystal and cryo-EM structures of Geobacillus kaustophilus and Bacillus subtilis T-box-tRNA complexes, detailing their multivalent, exquisitely selective interactions. The T-box forms a U-shaped molecular vise that clamps the tRNA, captures its 3' end using an elaborate 'discriminator' structure, and interrogates its aminoacylation state using a steric filter fashioned from a wobble base pair. In the absence of aminoacylation, T-boxes clutch tRNAs and form a continuously …

The Identification Of Long Non-Coding Rna Zfas1 Through An Exploratory Rna-Sequencing Analysis And Its Association With Epithelial-To-Mesenchymal Transition In Colon Cancer Adenocarcinoma., Stephen J. O'Brien

Electronic Theses and Dissertations

Colorectal adenocarcinoma is the fourth most common cancer diagnosed worldwide and is a significant cause of morbidity and mortality. This dissertation performed an exploratory RNA-sequencing analysis comparing gene expression between colon adenocarcinoma tissue and paired normal colon epithelium. After identification of a number of lncRNAs that were increased in expression in colon adenocarcinoma compared to normal colon epithelium, we aimed to validate the expression and investigate their function in vitro. Specifically, we focused on the lncRNA ZFAS1 and its association with epithelial-to-mesenchymal transition. These studies found the following: 1. Seven candidate lncRNAs were identified from the exploratory RNA-sequencing analysis to …

Quantitative Study Of The Antimicrobial Effects Of Silver On The Motility Of Escherichia Coli, Benjamin Russell

Graduate Theses and Dissertations

In recent decades, the number of antibiotic-resistant bacterial infections has grown to become a serious global threat. This rise can be attributed to the widespread misuse of antibiotics and the lack of newly developed drugs to fight resistant organisms. Novel bactericidal substances have, therefore, garnered significant research interest. Silver, due to its powerful antimicrobial effects, is one such substance. Silver is typically most effective in cationic form; however, advancements in nanotechnology have paved the way for the controlled fabrication of nano-silver. Silver nanoparticles have been shown to have increased antibacterial potency for a variety of reasons, including the release of …

Week Of November 25, 2019, New York Medical College

InTouch

• Accessibility Awareness and Education Lecture Focuses on Diabetes for National Diabetes Awareness Month
• School of Medicine Alumni Association Hosts Annual Reunion
• StuHE Hosts Career Mentor Session

Role Of Triphasic Computed Tomography In Prediction Of Response To Transcatheter Arterial Chemoembolization In Unresectable Hepatocellular Carcinoma Patients, Ahmed A.E Hassan Desoky, Hala M. Maghraby, Mohammed K. Omar

Journal of Medicine in Scientific Research

Background The initial approach in the management of hepatocellular carcinoma (HCC) is to determine if either surgical resection or liver transplantation is feasible. Unfortunately, more than three-quarters of the patients are diagnosed during the intermediate or advanced stages of the disease and are considered ineligible for curative resection. Transarterial chemoembolization (TACE) is the current standard of therapy for patients with intermediate-stage HCC according to the Barcelona Clinic Liver Cancer classification. Aim This study aims to evaluate the role of triphasic computed tomography (CT) in the prediction of the prognosis of irresectable HCC patients, who had been locally treated with TACE …

A Systematic Comparison Of Lipopolymers For Sirna Delivery To Multiple Breast Cancer Cell Lines: In Vitro Studies, Hamidreza Montazeri Aliabadi, Remant Bahadur Kc, Emira Bousoik, Ashley Barbarino, Bindu Thapa, Melissa Coyle, Parvin Mahdipoor, Hasan Uludağ

Pharmacy Faculty Articles and Research

Small interfering RNA (siRNA) therapy is a promising approach for treatment of a wide range of cancers, including breast cancers that display variable phenotypic features. To explore the general utility of siRNA therapy to control aberrant expression of genes in breast cancer, we conducted a detailed analysis of siRNA delivery and silencing response in vitro in 6 separate breast cancer cell models (MDA-MB-231, MDA-MB-231-KRas-CRM, MCF-7, AU565, MDA-MB-435 and MDA-MB-468 cells). Using lipopolymers for siRNA complexation and delivery, we found a large variation in siRNA delivery efficiency depending on the specific lipopolymer used for siRNA complexation and delivery. Some lipopolymers were …

Seasonal Plasticity In GabaA Signaling Is Necessary For Restoring Phase Synchrony In The Master Circadian Clock Network, Kayla Rohr, Harshida Pancholi, Shabi Haider, Christopher Karow, David Modert, Nicholas J. Raddatz, Jennifer A. Evans

Biomedical Sciences Faculty Research and Publications

Annual changes in the environment threaten survival, and numerous biological processes in mammals adjust to this challenge via seasonal encoding by the suprachiasmatic nucleus (SCN). To tune behavior according to day length, SCN neurons display unified rhythms with synchronous phasing when days are short, but will divide into two sub-clusters when days are long. The transition between SCN states is critical for maintaining behavioral responses to seasonal change, but the mechanisms regulating this form of neuroplasticity remain unclear. Here we identify that a switch in chloride transport and GABA_A signaling is critical for maintaining state plasticity in the SCN …