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Axillary Cystic Lymphangioma Presenting Inpregnancy, Fazıl Gelal, Kamil Yücel, Erdal Tuğsel, Şefik Güney

Turkish Journal of Medical Sciences

No abstract provided.

A Patient With Emaciation, Fever, Massivehepatosplenomegaly And Pancytopenia

Turkish Journal of Medical Sciences

No abstract provided.

Polyadenylation Of Vesicular Stomatitis Virus Mrna Dictates Efficient Transcription Termination At The Intercistronic Gene Junctions, Leroy N. Hwang, Nathan Englund, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The intercistronic gene junctions of vesicular stomatitis virus (VSV) contain conserved sequence elements that are important for polyadenylation and transcription termination of upstream transcript as well as reinitiation of transcription of downstream transcript. To examine the role of the putative polyadenylation signal 3' AUACU₇5' at the gene junctions in polyadenylation and transcription termination, we constructed plasmids encoding antigenomic minireplicons containing one or two transcription units. In plasmid-transfected cells, analyses of the bicistronic minireplicon containing the wild-type or mutant intercistronic gene junctions for the ability to direct synthesis of polyadenylated upstream, downstream, and readthrough mRNAs showed that the AUACU …

Factor Vii Deficiency Presenting Withmenometrorrhagia, Gürden Gür, Mete Özdoğan, Orhan Özgür, Sedat Boyacioğlu, Hasan Telatar

Turkish Journal of Medical Sciences

No abstract provided.

Patients' Rights In Turkey, Şükrü Hatun

Turkish Journal of Medical Sciences

Patient's rights primarily mean the practice of human rights and values in the field of health care services and include the rights which are considered as third generation rights. Recently, in many countries, especially in European countries, concept of health care services which give importance to patients' rights has been developed. Patients' rights in Turkey is one of the least known issues and remembered only by physicians' malparactises causing death or disabilities some of which are speculative. Efforts are needed to strengthen the patients' rights should be accelerated in order to protect the patients and physicians from unnecessary speculations in …

Mannoprotein Adhesin Of Candida Albicansgerm Tubes, Gülhan Vardar-Ünlü

Turkish Journal of Medical Sciences

The production and detection of a mannoprotein adhesin (MPA) of the hyphal-form cells of C. albicans on plastic petri dishes was investigated. Using Concanavalin A-coated latex microspheres, the MPA was detected on the plastic surface on which C. albicans produced germ tubes. The adhesin was extracted using dithiothreitol and iodoacetamide. It did not inhibit the adhesion of the yeast-form C. albicans to buccal epithelial cells (BEC). This suggested that the MPA of the hyphal-form cells on the plastic was different from that on the yeast cells responsible for attachment to the BECs.

The Role Of Ultrafiltration Therapy On Bloodpressure Of Hypertensive Chronic Hemodialysischildren, Caner Kabasakal, Sevgi Mi̇r, Ferah Sönmez, Nermin Beti̇n, Alpan Cura, Ruhi Özyürek

Turkish Journal of Medical Sciences

Hypervolemia is supposed to be the principal factor in hypertension in chronic hemodialysis adults. In this study, the role of intensive ultrafiltration (UF) treatment to reduce hypertension due to hypervolemia in hemodialysis children was investigated. The inferior vena cava (IVC) was also evaluated as an index of UF therapy. Twenty-three hemodialysis children with the mean age 10.6±3.1 years were classified due to their blood pressure status. IVC measurements were performed with 2-D echocardiography and inferior vena cava index (IVCI) was calculated as diameter of inferior vena cava (mm)/ body surface area (m 2 ). UF therapy was applied to each …

Medullary Thyroid Carcinoma: Review Of27 Cases, Yasemin Onaran, Harika Oguz, Tarık Terzi̇oğlu, Serdar Tezelman

Turkish Journal of Medical Sciences

Medullary thyroid carcinoma (MTC) may present in various clinical forms, either sporadic or a counterpart of hereditary endocrine disorders. The appropriate surgical procedure for MTC is total thyroidectomy combined with central neck clearance. The aim of this study was to document the initial clinical presentation, surgical treatment and clinical course of MTC in 27 patients. Thirteen (48.1%) cases were diagnosed as hereditary, either MEN 2a(7/27, 25.9%) or 2b syndrome (1/27, 3.7%) or familial non-MEN MTC (5/27, 18.5%), and fourteen cases were diagnosed as sporadic. Eighteen primary thyroid operations and twelve reoperative surgical procedures were performed on 24 patients. Eleven of …

The Role Of Leukotrienes In The Pathogenesis Ofsteroid-Sensitive Nephrotic Syndrome, Sevgi Mi̇r, Afig Hüseyi̇nov, Mehmet Kantar, Caner Kabasakal, Alphan Cura, Işıl Çoker

Turkish Journal of Medical Sciences

Endogenous leukotrienes (LT) levels in plasma and urine samples, and LT levels released from stimulated leukocytes in vitro were examined in children with steroid sensitive nephrotic syndrome (SSNS). Plasma LT concentrations were found to be higher in the SSNS group than in healthy subjects. Subjects with SSNS were also shown to have high levels of urinary cysteinyl-LTs and LTB 4 , while healthy children had only urinary LTE 4 levels. In the second part of the study, peripheral leukocytes of patients and heatlhy children were stimulated by Ca ionophore, A23187. We detected significantly higher levels of LTs in patients with …

Exclusion Mapping Of Polycystic Kidney Disease: A Third Locus, Eoin Thompson

Theses: Doctorates and Masters

The aim of this research was to perform exclusion on a rare form of hereditary autosomal dominant polycystic kidney disease (ADPKD). To-date, two genes for ADPKD have been identified: PKDI which has been localized to the short arm of chromosome 16 and PKD2 which has been localized to the long arm of chromosome 4. However, a small number of families have been reported that have not shown linkage to either of these two loci, thus suggesting the existence of at least one additional locus (PKD3). Two families that are affected with ADPKD and do not show linkage to PKDI or …

Molecular Analysis Of The Factor V-Leiden Mutation In A Cardiac Transplant Patient Population, Caroline Maher

Theses

Venous thrombosis is a serious health problem affecting 1 in 1000 individuals annually. Until recently the pathogenic factors underlying thrombosis were associated with genetic defects in protein C, protein S and antithrombin III. However these were recognised in fewer than 5-10% of thrombotic patients. A breakthrough was made with the discovery of activated protein C resistance (APCr) which is associated in 90% of cases v/herein a G-->A transition in the factor V gene results in an abnormal molecule, Factor V Leiden. APCr is a major risk factor for thrombosis being present in 20-60% of thrombotic patients. Recently a G-^A …

Faculty Publications 1997, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 767 publications.

Direct Demonstration Of Retroviral Recombination In A Rhesus Monkey, Dawn P. Wooley, Randall A. Smith, Susan Czajak, Ronald C. Desrosiers

Neuroscience, Cell Biology & Physiology Faculty Publications

Recombination may be an important mechanism for increasing variation in retroviral populations. Retroviral recombination has been demonstrated in tissue culture systems by artificially creating doubly infected cells. Evidence for retroviral recombination in vivo is indirect and is based principally on the identification of apparently mosaic human immunodeficiency virus type 1 genomes from phylogenetic analyses of viral sequences. We infected a rhesus monkey with two different molecularly cloned strains of simian immunodeficiency virus. One strain of virus had a deletion in vpx and vpr, and the other strain had a deletion in nef. Each strain on its own induced low virus …

The Effect Of Systemic Doxycycline On Alveolar Bone Loss Following Periradicular Surgery, G. Reed Cummings

Loma Linda University Electronic Theses, Dissertations & Projects

Studies have shown an average of 0.5 mm to over 1.0 mm of bone loss following full thickness flap reflection. Recent investigations have revealed that the tetracycline family of antibiotics can prevent bone loss. The purpose of this study was to determine the effect of systemic Doxycycline on crestal alveolar bone loss following periradicular surgery in Beagle dogs.

The experiment was divided into two phases. The first phase served as the control (Non-Doxycycline), the second phase as the experiment (Doxycycline). Full thickness flaps were reflected in the mandibular left or right quadrant (control side) of five Beagle dogs. Using a …

A Dichotomous Role For Nitric Oxide During Acute Toxoplasma Gondii Infection In Mice, Imtiaz A. Khan, Joseph D. Schwartzman, Tadashi Matsuura, Lloyd H. Kasper

Dartmouth Scholarship

Production of nitric oxide by macrophages is believed to be an important microbicidal mechanism for a variety of intracellular pathogens, including Toxoplasma gondii. Mice with a targeted disruption of the inducible nitric oxide synthase gene (iNOS) were infected orally with T. gondii tissue cysts. Time to death was prolonged compared with parental controls. Histologic analysis of tissue from infected mice showed scattered small foci of inflammation with parasites in various tissues of iNOS−/− mice, whereas tissue from the parental C57BL/6 mice had more extensive tissue inflammation with few visible parasites. In particular, extensive ulceration and necrosis of distal small …

Murine Transporter Associated With Antigen Presentation (Tap) Preferences Influence Class I-Restricted T Cell Responses., A J Yellen-Shaw, C E Laughlin, R M Metrione, Laurence C. Eisenlohr

Department of Biochemistry and Molecular Biology Faculty Papers

The transporter associated with antigen presentation (TAP) complex shuttles cytosolic peptides into the exocytic compartment for association with nascent major histocompatibility complex class I molecules. Biochemical studies of murine and human TAP have established that substrate length and COOH-terminal residue identity are strong determinants of transport efficiency. However, the existence of these specificities in the intact cell and their influences on T cell responses have not been demonstrated. We have devised a method for studying TAP- mediated transport in intact cells, using T cell activation as a readout. The approach makes use of a panel of recombinant vaccinia viruses expressing …

Phosphorylation Of Elongation Factor 1 And Ribosomal Protein S6 By Multipotential S6 Kinase And Insulin Stimulation Of Translational Elongation, Y W Chang, J A Traugh

Faculty, Staff and Student Publications

Stimulation of protein synthesis in response to insulin is concomitant with increased phosphorylation of initiation factors 4B and 4G and ribosomal protein S6 (Morley, S. J., and Traugh, J. A. (1993) Biochimie 75, 985-989) and is due at least in part to multipotential S6 kinase. When elongation factor 1 (EF-1) from rabbit reticulocytes was examined as substrate for multipotential S6 kinase, up to 1 mol/mol of phosphate was incorporated into the alpha, beta, and delta subunits. Phosphorylation of EF-1 resulted in a 2-2. 6-fold stimulation of EF-1 activity, as measured by poly(U)-directed polyphenylalanine synthesis. The rate of elongation was also …

Neurobehavior In Preterm Neonates Exposed To Cocaine, Alcohol, And Tobacco, K. A. Espy, M. L. Riese, D. J. Francis

Developmental Cognitive Neuroscience Laboratory: Faculty Publications

Neonatal neurobehavioral development was investigated in a sample of 20 hospitalized, prenatally cocaine-exposed preterm infants and 20 matched non-exposed controls. Prenatal cocaine exposure was related to reductions in attention performance that remained apparent at 36 wks conceptional age. There was no impact of prenatal cocaine exposure on the rate of change in attention proficiency. In utero alcohol exposure was associated with increased rates of age-related change in motor skill. Alcohol-related performance deficits were transient; alcohol-exposed infants reached an equivalent level of motor performance exhibited by the non-exposed infants by 36 wks conceptional age. These findings highlight the importance of considering …

The Shape School: Assessing Executive Function In Preschool Children, K. A. Espy

Developmental Cognitive Neuroscience Laboratory: Faculty Publications

Age-related changes in executive function were examined on a new task, the Shape School, in 70 preschool children (32-68 months old). The Shape School is a colorful storybook designed to examine inhibition and switching processes in young children. Results confirmed that task efficiency varied significantly with age, with older age groups outperforming younger groups. Furthermore, inhibition efficiency improved significantly between 3 to 4 years of age, whereas switching skills showed developmental improvement from 4 to 5 years of age. These results suggest that the Shape School is sensitive to maturational effects and that performance can be parsed into inhibition and …

Role Of Egr-1 Gene Expression In B Cell Receptor-Induced Apoptosis In An Immature B Cell Lymphoma, Subramanian Muthukkumar, Seong-Su Han, Sumathi Muthukkumar, Vivek M. Rangnekar, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Ligation of B cell receptor (BCR) on BKS-2, an immature B cell lymphoma by anti-IgM antibodies (Ab) caused apoptosis. Here we report that signaling through B cell receptor in wild type BKS-2 cells down-regulated the expression of Egr-1, a zinc finger-containing transcription factor. A reduction in the level ofEgr-1 mRNA could be demonstrated as early as 30 min after the ligation of BCR on BKS-2 cells. Immunocytochemical and Western blot analysis revealed that the expression of EGR-1 protein was also inhibited by anti-IgM treatment. Antisense oligonucleotides to Egr-1 caused growth inhibition and apoptosis in BKS-2 cells, suggesting that …

Right Medial Temporal-Lobe Contribution To Object-Location Memory, B Milner, Ingrid Johnsrude, J Crane

Brain and Mind Institute Researchers' Publications

An important aspect of normal human memory, and one humans share with many other species, is the ability to remember the location of objects in their environment. There is by now strong evidence from the study of epileptic patients undergoing brain surgery that right temporal-lobe lesions that encroach extensively upon the hippocampal and parahippocampal gyrus impair the delayed, but not the immediate, recall of the location of objects within a random array. These findings have now been extended to a multiple-trial, spatial-array learning task; by including not only patients tested after unilateral anterior temporal lobectomy but also those with a …

Spin Trapping Compounds, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Compositions containing as the active ingredient a spin-trapping reagent, preferably α-phenyl butyl nitrone (PBN) or spin-trapping derivatives thereof, in a suitable pharmaceutical carrier for administration to a patient are disclosed for treating or preventing symptoms associated with aging or other conditions associated with oxidative tissue damage. Other spin-trapping agents can also be used, such as 5,5-dimethyl pyrroline N-oxide (DMPO) or α-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN), and other spin-trapping derivatives thereof. These compositions and methods are useful in the treatment of age-related disorders, pre-surgical and/or pre-anesthetic preparation or administration of chemotherapeutic agents, and in the treatment of disorders or trauma of the brain, …

Triple Dissociation Of Anterior Cingulate, Posterior Cingulate, And Medial Frontal Cortices On Visual Discrimination Tasks Using A Touchscreen Testing Procedure For The Rat., T J Bussey, J L Muir, B J Everitt, T W Robbins

Brain and Mind Institute Researchers' Publications

Four experiments examined effects of quinolinic acid-induced lesions of the anterior cingulate, posterior cingulate, and medial frontal cortices on tests of visual discrimination learning, using a new "touchscreen" testing method for rats. Anterior cingulate cortex lesions impaired acquisition of an 8-pair concurrent discrimination task, whereas posterior cingulate cortex lesions facilitated learning but selectively impaired the late stages of acquisition of a visuospatial conditional discrimination. Medial frontal cortex lesions selectively impaired reversal learning when stimuli were difficult to discriminate; lesions of anterior and posterior cingulate cortex had no effect. These results suggest roles for the anterior cingulate, posterior cingulate, and medial …

Metabolically Inactive Insulin Analog Prevents Type I Diabetes In Prediabetic Nod Mice, Dennis G. Karounos, J. Scott Bryson, D. A. Cohen

Internal Medicine Faculty Publications

The purpose of this study was to determine the relative importance of the metabolic effects of insulin for diabetes prevention by administering insulin or an inactive insulin analog by daily subcutaneous injections to prediabetic mice. A recombinant monomeric human insulin analog, which does not bind to the insulin receptor as a consequence of an alteration of a single amino acid at position 25 of the B chain, was shown to be equally effective at diabetes prevention as was intact insulin. In contrast to native insulin, the insulin analog did not cause hypoglycemia after subcutaneous injection. The insulin analog, however, protected …

Stent Placement Compared With Balloon Angioplasty For Obstructed Coronary Bypass Grafts. Saphenous Vein De Novo Trial Investigators., M P Savage, J S Douglas, D L Fischman, C J Pepine, S B King, J A Werner, S R Bailey, P A Overlie, S H Fenton, J A Brinker, M B Leon, S Goldberg

Department of Medicine Faculty Papers

BACKGROUND: Treatment of stenosis in saphenous-vein grafts after coronary-artery bypass surgery is a difficult challenge. The purpose of this study was to compare the effects of stent placement with those of balloon angioplasty on clinical and angiographic outcomes in patients with obstructive disease of saphenous-vein grafts.

METHODS: A total of 220 patients with new lesions in aortocoronary-venous bypass grafts were randomly assigned to placement of Palmaz-Schatz stents or standard balloon angioplasty. Coronary angiography was performed during the index procedure and six months later.

RESULTS: As compared with the patients assigned to angioplasty, those assigned to stenting had a higher rate …

Mitotic Spindle Poles Are Organized By Structural And Motor Proteins In Addition To Centrosomes, Tirso Gaglio, Mary A. Dionne, Duane A. Duane A. Compton

Dartmouth Scholarship

The focusing of microtubules into mitotic spindle poles in vertebrate somatic cells has been assumed to be the consequence of their nucleation from centrosomes. Contrary to this simple view, in this article we show that an antibody recognizing the light intermediate chain of cytoplasmic dynein (70.1) disrupts both the focused organization of microtubule minus ends and the localization of the nuclear mitotic apparatus protein at spindle poles when injected into cultured cells during metaphase, despite the presence of centrosomes. Examination of the effects of this dynein-specific antibody both in vitro using a cell-free system for mitotic aster assembly and in …

The Type 2 Iodothyronine Deiodinase Is Expressed Primarily In Glial Cells In The Neonatal Rat Brain, Ana Guadaño-Ferraz, Maria Jesus Obregón, Donald L. St. Germain, Juan Bernal

Dartmouth Scholarship

Thyroid hormone plays an essential role in mammalian brain maturation and function, in large part by regulating the expression of specific neuronal genes. In this tissue, the type 2 deiodinase (D2) appears to be essential for providing adequate levels of the active thyroid hormone 3,5,3′-triiodothyronine (T3) during the developmental period. We have studied the regional and cellular localization of D2 mRNA in the brain of 15-day-old neonatal rats. D2 is expressed in the cerebral cortex, olfactory bulb, hippocampus, caudate, thalamus, hypothalamus, and cerebellum and was absent from the white matter. At the cellular level, D2 is expressed predominantly, if not …

Sqt1, Which Encodes An Essential Wd Domain Protein Of Saccharomyces Cerevisiae, Suppresses Dominant-Negative Mutations Of The Ribosomal Protein Gene Qsr1., Dominic P. Eisinger, Frederick A. Dick, Elke Denke, Bernard L. Trumpower

Dartmouth Scholarship

QSR1 is an essential Saccharomyces cerevisiae gene, which encodes a 60S ribosomal subunit protein required for joining of 40S and 60S subunits. Truncations of QSR1 predicted to encode C-terminally truncated forms of Qsr1p do not substitute for QSR1 but do act as dominant negative mutations, inhibiting the growth of yeast when expressed from an inducible promoter. The dominant negative mutants exhibit a polysome profile characterized by 'half-mer' polysomes, indicative of a subunit joining defect like that seen in other qsr1 mutants (D. P. Eisinger, F. A. Dick, and B. L. Trumpower, Mol. Cell. Biol. 17:5136-5145, 1997.) By screening a high-copy …

Increased Focal Adhesion Kinase- And Urokinase-Type Plasminogen Activator Receptor-Associated Cell Signaling In Endothelial Cells Exposed To Asbestos, A Barchowsky, B M. Lannon, L C. Elmore, M D. Treadwell

Dartmouth Scholarship

Exposure of low-passage endothelial cells in culture to nonlethal amounts of asbestos, but not refractory ceramic fiber-1, increases cell motility and gene expression. These changes may be initiated by the fibers mimicking matrix proteins as ligands for receptors on the cell surface. In the present study, 1- to 3-hr exposures of endothelial cells to 5 mg/cm2 of chrysotile asbestos caused marked cell elongation and motility. However, little morphological change was seen when chrysotile was added to cells pretreated with either mannosamine to prevent assembly of glycophosphatidylinositol (GPI)-anchored receptors or with herbimycin A to inhibit tyrosine kinase activity. Affinity purification of …

Qsr1p, A 60s Ribosomal Subunit Protein, Is Required For Joining Of 40s And 60s Subunits., Dominic P. Eisinger, Frederick A. Dick, Bernard L. Trumpower

Dartmouth Scholarship

QSR1 is a recently discovered, essential Saccharomyces cerevisiae gene, which encodes a 60S ribosomal subunit protein. Thirty-one unique temperature-sensitive alleles of QSR1 were generated by regional codon randomization within a conserved 20-amino-acid sequence of the QSR1-encoded protein. The temperature-sensitive mutants arrest as viable, large, unbudded cells 24 to 48 h after a shift to 37 degrees C. Polysome and ribosomal subunit analysis by velocity gradient centrifugation of lysates from temperature-sensitive qsr1 mutants and from cells in which Qsr1p was depleted by down regulation of an inducible promoter revealed the presence of half-mer polysomes and a large pool of free 60S …