Digital Commons Network^™

Accessory Limb Attached To The Back, Di̇dem Akyol, Volkan Baltaci, Hakan Kozi̇noğlu, Kutlugül Yüksel, Sai̇me Kiş, Nedi̇m Çi̇çek, Tayfun Güngör

Turkish Journal of Medical Sciences

No abstract provided.

Review Of: Lewis' Dictionary Of Toxicology (Robert A. Lewis, Ed.; Lewis Publishers 1998), Dana E. Evans

RISK: Health, Safety & Environment (1990-2002)

Review of the book: Lewis' Dictionary of Toxicology (Robert A. Lewis, ed.; Lewis Publishers 1998). About the author, acknowledgments, alphabetical listing of terms defined. ISBN 1-56670-223-2; [1127 pp. $84.95. Hardbound. 2000 Corporate Blvd. N.W., Boca Raton, FL 33431.]

Molecular Studies Of Splice Sites In The Canine Dystrophin Gene, Hayley Durling

Theses : Honours

The development of an effective therapy for Duchenne Muscular Dystrophy (DMD) is one of the primary goals of all DMD/Becker Muscular Dystrophy (BMD) research. Golden Retriever Muscular Dystrophy (GRMD), an animal model of DMD is a fatal degenerative myopathy. Unlike the mdx model, the GRMD dog more accurately reflects the phenotype shown by human DMG patients, making the model better suited for the investigation and assessment of potential therapeutic approaches. The GRMD mutation, a base change from A to G in the 3' splice acceptor site of intron 6, results in exon 7 skipping which disrupts the translational reading frame. …

Plasmd Bearing A Cdna Copy Of The Genome Of Bovine Viral Diarrhea Virus, Chimeric Derivatives Thereof, And Method Of Producing An Infectious Bovine Wral Darrheavirus Using Sad Plasmid, Ruben O. Donis, Ventzislav B. Vassilev

School of Veterinary and Biomedical Sciences: Faculty Publications

A plasmid bearing a cDNA copy of the genome of bovine viral diarrhea virus (BVDV), chimeric derivatives of the plasmid and a method of producing an infectious bovine viral diarrhea virus using the plasmid are disclosed. The invention relates to a plasmid DNA molecule that replicates easily in E. coli and contains a sufficient portion of the genome of BVDV, cloned as cDNA, to be a suitable template to produce RNA in vitro which, upon transfection into bovine cells, gives rise to infectious BVDV. The BVDV created by the process of the invention can be engineered for use as a …

In Situ Hybridization For The Detection And Localization Of Swine Chlamydia Trachomatis, C. Chae, D.-S. Cheon, D. Kwon, O. Kim, B. Kim, J. Suh, D. G. Rogers, K. D. E. Everett, A. A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

Gnotobiotic piglets were inoculated intralaryngeally with swine Chlamydia trachomatis strain R33 or orally with swine C. trachmatis strain R27. Archived formalin-fixed, paraffin-embedded tissues from piglets euthanatized 4–7 days postinoculation were examined by in situ hybridization for C. trachomatis nucleic acid using a nonradioactive digoxigenin-labeled DNA probes that targeted specific ribosomal RNA or omp1 mRNA molecules of the swine C. trachomatis strains. Positive hybridization signals were detected in bronchial epithelial cells, bronchiolar epithelial cells, pneumocytes, alveolar and interstitial macrophages, and jejunal and ileal enterocytes. Chlamydia-infected cells had a strong signal that was confined to the intracytoplasmic inclusions. Positive hybridization signals were …

Potential Associations Among Genetic Markers In The Serotonergic System And The Antisocial Alcoholism Subtype, E. M. Hill, S. F. Stoltenberg, M. Burmeister, M. Closser, R. A. Zucker

Department of Psychology: Faculty Publications

Alcoholism is transmitted in families. The complexity and heterogeneity of this disorder has made it difficult to identify specific genetic correlates. One design with the potential to do so is the family-based association study, in which the frequencies of genetic polymorphisms are compared between affected and nonaffected members. Reduced central serotonin neurotransmission is associated with features of an antisocial subtype of alcoholism, although a primary deficit has not been traced to a particular component. Genetic markers related to the serotonergic system have been identified, located, and cloned. If associations can be discovered, the development process for pharmacotherapy could be facilitated. …

The Effect Of Fish Oil On Liver And Plasma Mda And Antioxidant Status Of Rats, Nuriye Mete, Birgül Işik, Levent Erdi̇nç, Fuat Gürkan

Turkish Journal of Medical Sciences

The question how fish oil (FO) and soybean oil (SO) change the state of antioxidant and the lipid peroxidation in liver and plasma of rats and the protective role of vitamin E were investigated. Six-week-old forty male Sprague-Dawley rats were divided into five groups. One of the groups fed a control diet, another one fed SO, while the other three groups fed FO diet at the level of 10% Vitamin E supplementation was maintained to three different levels 3, 50, 150 IU/per kg diet in the FO diets and 3 IU vitamin E per kg of the SO diet. At …

Quantification Of Total Collagen In Rabbit Tendonby The Sirius Red Method, Dilek Taşkiran, Emin Taşkiran, Hüseyin Yercan, Fatma Z. Kutay

Turkish Journal of Medical Sciences

No abstract provided.

Genitourinary Abnormalities In Children With Urinary Tract Infections, Ayşe Balat, L. Leighton Hill

Turkish Journal of Medical Sciences

Urinary-tract infections (UTI) are common infectious diseases in childhood. The presence of UTI may be an indicator of a serious genitourinary (GU) abnormality that requires medical intervention. We retrospectively reviewed the charts of children discharged with a diagnosis of UTI from the Texas Children's Hospital, Houston, Texas, between July 1, 1991 and June 30, 1994. The purpose of the study was to determine how many patients had underlying GU abnormalities, the specific type of abnormality, and the microorganism causing the UTI. During the 3-year period, there were 48,382 discharges from the hospital, and 1,221 (2.5%) of those discharges were patients …

Effect Of Exercise On Blood Antioxidant Status And Erythrocyte Lipid Peroxidation: Role Of Dietary Supplementation Of Vitamin E, Mustafa Kelle, Hüda Di̇ken, Abdurrahman Şermet, Mukadder Atmaca, Cemi̇l Tümer

Turkish Journal of Medical Sciences

We tested the effects of moderate physical exercise on the blood antioxidant capacity and erythrocyte lipid peroxidation in 21 Wistar albino rats. Erythrocyte superoxide dismutase (SOD) activity increased significantly (p

Administration Of Granulocyte Colonystimulating Factor (G-Csf) In Experimentallyinfected Rats, Ömer Cevi̇t, Ruhi̇ye Cevi̇t, Gökhan Köylüoğlu, Di̇lara İçağasioğlu, Asim Gülteki̇n, Fatoş Tanzer

Turkish Journal of Medical Sciences

We investigated the effect of antibiotic or G-CSF plus antibiotic therapy given to E. coli septic rats. In our study, 80 rats were inoculated transperitoneally with 1 ml of a solution containing 10 7 colony forming units per milliliter of E. coli. Rats were divided into four treatment groups before being inoculaded with E. coli in a random fashion. Therapy was then started with cefotaxime (150 mg/kg intraperitonelally) and either Granulocyte-Colony Stimulation Factor (G-CSF; 30 µg/kg intarperitoneally) or placebo 24 hours after inoculation of E. coli for 3 consective days. Group A 1 , sepsis-antibiotics group, had a mortality rate …

A Patient With Three Simultaneously Occurring Malignancies, Bahar Kiliçarslan, Gülay Özbi̇li̇m, Gökbay Elpek, Şeyda Karaveli̇, Mi̇ne Üner

Turkish Journal of Medical Sciences

No abstract provided.

The Evaluation Of Microanastomoses In Rats Withcolor Doppler Ultrasonography, Bahri̇ Üstünsöz, İbrahi̇m Somuncu, Mahmut Kömürcü, Mustafa Başşbozkurt, Sabri̇ Ateşalp, Alper Alemdaroğlu

Turkish Journal of Medical Sciences

Purpose: To evaluate the vascular patency of microanastomoses in rats with color Doppler ultrasonography. Materials and Methods: 15 superficial femoral and 15 carotid arterial end-to-end anastomoses were performed in 30 white rats. All arteries were evaluated pre- and post-operatively by color Doppler ultrasonography with a 7.5 mHz linear transducer (Acuson 128HP). All results were correlated with surgery after final Doppler evaluation. Results: The subjects were divided into three groups: (a) patent anastomosis; (b) partial occlusive anastomosis; (c) total occlusive anastomosis. The predictive value ratios for the patency group was 100% (15/15), that for the partial occlusive group was 67% (6/9) …

The In Vitro Effect Of Plasmodium Bergheiantigen Lysate On Natural Killer (Nk) Cellcytotoxic Activity, Cemaletti̇n Albayi̇, Ayşegül Yücel, Kemal Çeber, Turgut İmi̇r

Turkish Journal of Medical Sciences

Plasmodium berghei is the rodent equivalent of Plasmodium falciparum which causes cerebral malaria in humans. However, P. berghei does not cause cerebral malaria in BALB/c mice, instead it leads to parasitemia, fever and some degree of liver damage. Although different aspects of immunological response have been intensively investigated in malaria; this the first time the in vitro effect of P. berghei antigen lysate on murine NK cytotoxic activity was studied. It was observed that this parasite antigen lysate suppresed the cytolytic activity of NK cells in a dose-dependent manner. Because the suppression of NK cell activity in a dose-dependent manner …

Generalized Bcg Lymphadenitis In An Infant:Diagnostic Dilemma With Lepromatous Leprosyand Gaucher Disease On Fine Needle Aspiration, Mustafa Hacimustafaoğlu, Mehmet Okan, Ömer Yerci̇, Suna Gedi̇koğlu, İrfan Kiriştioğlu, İbrahi̇m Ildirim

Turkish Journal of Medical Sciences

A two-year old male infant with generalized BCG lymphadenitis is presented. There was no obvious immunodeficiency disorder detected in the patient. BCG vaccine had been given at two months of age, and the lymphadenopathy developed at 21 months of age. Fine needle aspiration biopsy of the lymph node on Giemsa stain revealed macrophages very similar to Gaucher cells. These cells also resembled macrophages seen in lepromatous leprosy. They were engorged with acid fast bacilli and had streaked or wrinkled cytoplasm. Mycobacterium bovis was cultured from the lymph nodes and the patients improved with antituberculous therapy.

Nadph-Diaphorase-Reactive Neurons In Rat Basal Ganglia, Gülgün Kayalioğlu, Fi̇gen Gövsa, Mete Ertürk

Turkish Journal of Medical Sciences

NADPH-d reaction was performed on nine male Sprague-Dawley rats and morphometry of NADPH-d(+) cells was investigated in caudate-putamen, globus pallidus and ventral pallidum. In caudate-putamen we observed intensely stained NADPH-d (+) cells. Maximum diameter and minimum diameters of the NADPH-d (+) cells in caudate-putamen was measured as 19.19±0.04 \mum and 10.42±0.12 \mum, consequently. In globus pallidus, moderately stained NADPH-d (+) cells were observed. We measured the maximum and minimum diameters of the NADPH-d (+) cells as 19.93±0.24 \mum and 10.42±0.12 \mum, consequently. In ventral pallidum, medium-sized, moderately stained cells were present. Maximum and minimum diameters of the NADPH-d (+) cells …

Protein C, Protein S And Antithrombin Iii Levelsin A Rabbit Sepsis Model, Mehmet Totan, Cengi̇z Di̇lber, Davut Albayrak

Turkish Journal of Medical Sciences

The consumption of coagulation inhibitors will augment coagulation triggered excessively in sepsis. The determination of levels of these inhibitors may open new substitution treatment schedules to overcome the plateau of the achieved improvement ratio with antimicrobial treatment. Staphylococcus aureus ATCC 25923, 1x10 9 per kg day for two days was administered intravenously to 30 albino rabbits to induce sepsis. Clinical and hematological signs were determined and sepsis was determined with blood culture. The complete blood count, protein C, protein S and antithrombin III levels were examined at the baseline and after sepsis development. The mean proportions of baseline to sepsis …

Immunohistochemical Study On Thepcna-Immunoreactivity In The Uterus Of Ratsovariectomized Or Treated With Antiestrogenclomiphene Citrate, Leyla Canpolat, İbrahi̇m Hani̇fi̇ Özercan, Nurten Bozlak, Cengi̇z Doğan

Turkish Journal of Medical Sciences

PCNA (proliferating cell nuclear antigen) immunoreactivity in the uterus of mature rats was investigated after long term administration of clomiphene citrate and ovariectomy. In the luminal epithelium and glandular epithelium and endometrial stroma regions, few PCNA-immunoreactive cells were observed. PCNA reactivity was estimated for the luminal and glandular epithelium and for the stromal cells. Treatment of clomiphene citrate decreased the proliferation of the luminal epithelial cell and stromal cells to the same extent as ovariectomy. In both of these groups, the proportion of anti-PCNA positive cells in the glandular epithelium was significantly higher than in the luminal epihelium and stromal …

Determination Of Viable Myocardium In Patientswith Ischaemic Left Ventricular Dysfunction:Comparison Of Thallium-201 Single-Photonemission Computed Tomography With Nitrateand Dobutamine Echocardiography, Mustafa Cemri̇, Ridvan Yalçin, Bülent Boyaci, Özlem Kapucu, Tamer Atasever, Ati̇ye Çengel, Övsev Dörtlemez, Hali̇s Dörtlemez

Turkish Journal of Medical Sciences

The method of Thallium - 201- single - photon emission computed tomography (Tl-201 SPECT) have been commonly used to detect uncontractile but viable myocardium in patients with ischemic left ventricular dysfunction. The aim of this study was to establish the value of nitrate echocardiography (NE) and low dose dobutamine echocardiography (LDDE) to detect viable myocardium when compared to Tl-201 SPECT. Twently patients (16 M, 4F; mean age: 56.4±9.6 years) who had anterior myocardial infarction in the last three months were studied. Within the same week, but on different days NE, LDDE and Tl-201 SPECT procedures were performed to the patients. …

Neutrophil Chemotaxis In Acutely Infected Andclinically Stable Cystic Fibrosis Patients, Burçi̇n Şener, Gülşen Hasçeli̇k, Uğur Özçeli̇k, Ayfer Günalp, Ayhan Göçmen

Turkish Journal of Medical Sciences

Brochopulmonary infection in cystic fibrosis (CF) patients is associated with chronic progressive lung disease. The role of host factors, such as neutrophil functions, in the progressive pathologic processes in the respiratory tract of these patients, is poorly defined. Neutrophil chemotaxis is one of the first mechanisms of host defense to become activated after bacterial invasion has occurred. This study was aimed to evaluate the role of neutrophil chemotaxis in CF and also to determine whether an acute bacterial infection and the nutritional status can affect neutrophil chemotaxis or not. Twelve acutely infected and 12 clinically stable CF patients and 10 …

Dose Dependent Changes In The Cristaneuroepitheium Resulting From Gentamicinototoxicity In The Chinchilla, Hasan Tanyeri̇, Ivan Lopez, Larry Hoffman

Turkish Journal of Medical Sciences

This experiment was conducted to investigate the dose-dependent effects of gentamicin (GM) on the neuroepithelial morphology of the posterior crista ampullares (PCA) of the adult chinchilla. The experiment focused on hair cell (HC) morphology to monitor GM's dose-dependent effects and also to define an ototoxically-efficient and safe dose what would subsequently be used in a time course study. Chinchillas (n=13) were treated with subcutaneous (SC) daily doses of 120 mg/kg, 60 mg/kg, and 30 mg/kg GM, respectively, for six days. Light microscopy observations were made from the excised PCA of two animals from each group that survided the complete treatment …

The Effects Of Smoking On Glutathione Levels Inbronchoalveolar Lavage Fluid, Füsun Alataş, Necla Özdemi̇r, Özkan Alataş, Si̇nan Ergi̇nel, Muzaffer Meti̇ntaş, Rana Işik

Turkish Journal of Medical Sciences

Cigarette smoke contains high concentrations of oxidants and reactive oxygen species. The aim of this study was to evaluate the effect of long-term cigarette smoking on glutathione (GSH) levels in bronchoalveolar lavage (BAL) fluid. Nineteen current cigarette smokers and seven healthy nonsmoking volunteers were included in the study. BAL was performed by infusion of five sequential 20 ml aliquots and each aliquot was immediately aspirated. The levels of GSH were determined in BAL fluids. The epithelial lining fluid volume was calculated by the ratio of urea concentration in BAL fluid to that in serum. Lower GSH levels were observed in …

A Combined Variation Of The Arteries And The Nerve In A Leg, Zeli̇ha Kurtoğlu, M. Haluk Uluutku

Turkish Journal of Medical Sciences

During the dissection of an amputed leg, we discovered a combined variation involving the branching of the popliteal artery and tibial nerve. The popliteal artery showed a trifurcation pattern, and one of its branches, the posterior tibial artery, was hypoplastic. In addition, a small branch arising from the tibial nerve in the upper part of the leg distally crossed the peroneal artery and contributed to the medial plantar nerve. In this article, such a case of embryonic origin is discussed, along with the clinical significance of these variations. The variant nerve and its relationship with the variant artery have not …

Faculty Publications 1998, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 822 publications.

Regeneration Of Hair Cell Epithelia In The Chick And Salamander, Kenneth Detwiler

Masters Theses & Specialist Projects

The acousticolateralis sensory system is characterized by a specific receptor cell type called the sensory hair cell and is found in all vertebrates. There are two types of hair cell sensory epithelia based on location: those of the inner ear, such as the organs for hearing and balance, and the lateral line system located within the epidermis. In mammals, including humans, loss or damage of the hair cells of the auditorysystem results in permanent hearing loss. However, this is not the case with birds and amphibians. Amphibians, with a lateral line system, are capable of replacing lost or damaged hair …

The Isolation And Partial Purification Of Dna Polymerase-Beta From Soybean, Lesa Dill

Masters Theses & Specialist Projects

5 kilodaltons). Its activation and inhibition spectra were similar to those previously determined for animal polymerase-beta. Therefore, since the structure of polymerase-beta shows significant evolutionary conservation in both plant and animal species and given strong functional correlations among species, polymerase-beta performs a vital role in plants in the repair of newly synthesized DNA just as it does in animal systems.

Identification And Characterization Of Spcu, A Chaperone Required For Efficient Secretion Of The Exou Cytotoxin, Viviane Finck-Barbançon, Timothy L. Yahr, Dara W. Frank

Dartmouth Scholarship

In recent studies, we have shown that Pseudomonas aeruginosa strains that are acutely cytotoxic in vitro damage the lung epithelium in vivo. Genetic analysis indicated that the factor responsible for acute cytotoxicity was controlled by ExsA and therefore was part of the exoenzyme S regulon. The specific virulence determinant responsible for epithelial damage in vivo and cytotoxicity in vitro was subsequently mapped to the exoU locus. The present studies are focused on a genetic characterization of the exoU locus. Northern blot analyses and complementation experiments indicated that a region downstream of exoU was expressed and that the expression of this …

The Role Of Gap Junctions In Congenital Diseases Of The Heart, Scott Henry Britz-Cunningham

Loma Linda University Electronic Theses, Dissertations & Projects

Background. Gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. We hypothesized that mutations altering sites critical to this regulation would lead to functional or developmental abnormalities of the heart.

Methods. Connexin43 DNA from 25 normal subjects and 30 children with a variety of congenital heart diseases was amplified by the polymerase chain reaction and sequenced. Mutant DNA was expressed in cell culture and examined for its effect …

Staphylococcal Enterotoxin B Primes Cytokine Secretion And Lytic Activity In Response To Native Bacterial Antigens, K. M. Mason, T. D. Dryden, Nancy J. Bigley, P. S. Fink

Neuroscience, Cell Biology & Physiology Faculty Publications

Superantigens stimulate T-lymphocyte proliferation and cytokine production, but the effects of superantigen exposure on cell function within a complex, highly regulated immune response remain to be determined. In this study, we demonstrate that superantigen exposure significantly alters the murine host response to bacterial antigens in an in vitro coculture system. Two days after exposure to the superantigen staphylococcal enterotoxin B, splenocytes cultured with Streptococcus mutans produced significantly greater amounts of gamma interferon (IFN-gamma) and interleukin-12 than did sham-injected controls. The majority of IFN-gamma production appeared to be CD8(+) T-cell derived since depletion of this cell type dramatically reduced the levels …

The Seca Subunit Of Escherichia Coli Preprotein Translocase Is Exposed To The Periplasm, Jerry Eichler, William Wickner

Dartmouth Scholarship

SecA undergoes conformational changes during translocation, inserting domains into and across the membrane or enhancing the protease resistance of these domains. We now show that some SecA bound at SecYEG is accessible from the periplasm to a membrane-impermeant probe in cells with a permeabilized outer membrane but an intact plasma membrane.