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Expression Of The 8 Kda Heat Shock Protein (Ubiquitin) In Psoriasis, İbrahi̇m Pi̇ri̇m, Teoman Erdem, Necmetti̇n Akdeni̇z, Yaşar Nuri̇ Şahi̇n Jan 2001

Expression Of The 8 Kda Heat Shock Protein (Ubiquitin) In Psoriasis, İbrahi̇m Pi̇ri̇m, Teoman Erdem, Necmetti̇n Akdeni̇z, Yaşar Nuri̇ Şahi̇n

Turkish Journal of Medical Sciences

The 8kDa protein (ubiquitin) is a member of the small heat shock protein (hsb) family. Ubiquitin may additionally be involved in the pathogenesis of various diseases. Intracellular accumulation of ubiquitin has been detected in patients with neurodegenerative disease, brain ischemia, cancer, rheumatoid arthritis and haemodialysis. Sixteen biopsy samples from patients with psoriasis who were treated by PUVA and the same samples from these patients before PUVA treatment were investigated by immunohistochemistry on formalin-fixed paraffin- embedded tissue sections, using anti-ubiquitin antibody. In psoriatic epidermis, ubiquitin was mostly expressed in basal layers with a cytoplasmic staining pattern. Various cells of the dermis …

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Pulmonary Infections Due To Legionella Pneumophila In Immunocompromised Patients, Demet Kaya, Si̇nan Ergi̇nel, Muzaffer Meti̇ntaş, Zeki̇ Üstüner, İrfan Uçgun, Necla Özdemi̇r Jan 2001

Pulmonary Infections Due To Legionella Pneumophila In Immunocompromised Patients, Demet Kaya, Si̇nan Ergi̇nel, Muzaffer Meti̇ntaş, Zeki̇ Üstüner, İrfan Uçgun, Necla Özdemi̇r

Turkish Journal of Medical Sciences

The numbers of immunocom-promised individuals increase as programmes for organ transplantation and chemotherapy of malignant disease advance. Pulmonary infections, which progress rapidly, are one of the major clinical problems in these groups. This study was designed to evaluate the microbiology of pneumonia in immunocompromised patients and to determine the role of Legionella pneumophila. Immunocompromised patients receiving antineoplastic chemotherapy, diagnosed as pneumonia with clinical and radiographic findings and routine laboratory tests were included in the study. For microbiological diagnosis, expectorated sputum was cultured for L. pneumophila. In addition to standard bacterial, fungal and mycobacterial media, specimens were examined microscopically. Direct flourescent …

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Perioperative Oral Supplement With Immunonutrients In Gastrointestinal Cancer Patients, Ni̇hal Zeki̇ye Erdem, İ. Hakan Kulaçoğlu, Mehtap Akci̇l Temel, Ahmet Çinar Yasti, Nuri̇ Aydin Kama, Nazan Bozkurt Jan 2001

Perioperative Oral Supplement With Immunonutrients In Gastrointestinal Cancer Patients, Ni̇hal Zeki̇ye Erdem, İ. Hakan Kulaçoğlu, Mehtap Akci̇l Temel, Ahmet Çinar Yasti, Nuri̇ Aydin Kama, Nazan Bozkurt

Turkish Journal of Medical Sciences

Significant benefits have been gained with pre or peri-operative nutritional support in surgical patients with malnutrition. Recent studies have also shown that some enteral formulas including certain nutrients like glutamine could provide more benefit than the standard formulas. In this prospective-randomized study, the effects of perioperative oral supplement with enteral formulas containing glutamine in comparison with a standard enteral formula in patients who were operated on for GI malignancies were examined. Thirty-two patients were divided into two groups: the study group was given oral supplement with an elemental diet (Alitraq®) for 7 days preoperatively and 10 days postoperatively as 30-35% …

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Observations Of The Changes Occurring In The Rat Trachea Due To Inhalation Of Cigarette Smoke, Aysel Kükner, Hakan Öner, Neri̇man Çolakoğlu, Jale Öner, Enver Ozan, Sema Ozan, Seval Yilmaz Jan 2001

Observations Of The Changes Occurring In The Rat Trachea Due To Inhalation Of Cigarette Smoke, Aysel Kükner, Hakan Öner, Neri̇man Çolakoğlu, Jale Öner, Enver Ozan, Sema Ozan, Seval Yilmaz

Turkish Journal of Medical Sciences

In this study, the tracheal tissues of rats inhaling cigarette smoke passively during different periods were examined by light and electron microscopy. Malonaldehyde, erythrocyte catalase and arginase levels were also measured. Three groups were formed and a total of 15 Wistar male rats weighing 200-250 g were used. Rats assigned to the first and second groups were made to inhale cigarette smoke for 30 and 60 minutes respectively for a total of 3 months. The subjects in the third group were made to inhale clean air as a control. Cigarettes without filter tips were used througout the study. Lit cigarettes …

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Smooth Muscle Actin (Sma) Immunoreactivity In Primary Glomerulonephritis (Gn), Duygu Düşmez, Gülfi̇li̇z Gönlüşen, Emel Akoğlu, Sevgi̇ Küllü, İlhan Tuncer Jan 2001

Smooth Muscle Actin (Sma) Immunoreactivity In Primary Glomerulonephritis (Gn), Duygu Düşmez, Gülfi̇li̇z Gönlüşen, Emel Akoğlu, Sevgi̇ Küllü, İlhan Tuncer

Turkish Journal of Medical Sciences

The aims of this study were to determine the role of interstitial and glomerular myofibroblasts in the progression of various glomerulonephritis, and to assess the value of myofibroblasts in determining long-term renal outcome. The biopsy specimens of 42 primary glomerulonephritis cases were reviewed and stained immunohistochemically with alpha smooth muscle actin antibody. The serum creatine level was compared to the pathological changes. The interstitial alpha smooth muscle actin staining correlated with the serum creatine level at the time of biopsy. The glomerular staining, conversely, did not show any relation with renal function. Interstitial myofibroblasts play a role in the development …

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A Study On The Rat Stapedial Artery Under The Dissection Microscope, Cahi̇de Ortuğ Jan 2001

A Study On The Rat Stapedial Artery Under The Dissection Microscope, Cahi̇de Ortuğ

Turkish Journal of Medical Sciences

The stapedial artery is a rare anomaly in man. However, an undetected persistence of the artery may cause problems during middle ear surgery. The studies on its course are inadequate. The aim of the present paper is to describe the course of the stapedial artery in rats, through an examination under a dissection microscope.

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The Effect Of Sulfur Dioxide On T-Butyl Hydroperoxide Induced Oxidative Stress In Lung Tissues Of Rats, Saadet Gümüşlü, Di̇jle Ki̇pmen Korgun, Süreyya Bi̇lmen, Gülteki̇n Yücel, Pi̇raye Yargiçoğlu, Aysel Ağar Jan 2001

The Effect Of Sulfur Dioxide On T-Butyl Hydroperoxide Induced Oxidative Stress In Lung Tissues Of Rats, Saadet Gümüşlü, Di̇jle Ki̇pmen Korgun, Süreyya Bi̇lmen, Gülteki̇n Yücel, Pi̇raye Yargiçoğlu, Aysel Ağar

Turkish Journal of Medical Sciences

In this study, the effects of sulfur dioxide (SO_2) ,a common pollutant in urban air, on t-butyl hydroperoxide-induced oxidative stress in rat lung tissues were examined. Ten young rats and 10 adult rats were exposed to SO_2 while another 10 young rats and 10 adult rats made up the control groups. Ten ppm SO_2 was administered to the animals of the SO_2 groups in an exposure chamber for one hour a day every day for 6 weeks. The control groups were exposed to filtered air in the same chamber for the same period. At the end of the experimental period, …

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In Vivo Interaction Between Cadmium And Essential Trace Elements Copper And Zinc In Rats, H. Hakan Aydin, Canan Çoker, Bi̇ltan Ersöz Jan 2001

In Vivo Interaction Between Cadmium And Essential Trace Elements Copper And Zinc In Rats, H. Hakan Aydin, Canan Çoker, Bi̇ltan Ersöz

Turkish Journal of Medical Sciences

The complex in vivo interaction between Cd, a toxic metal and essential trace elements, mainly Zn and Cu, has not been elucidated yet. The objective being the elucidation of this interrelationship, Cd was subcutaneously administered as CdCI_2 (1 mg/kg/day) for 5 consecutive days to Swiss albino male rats (n=10). After 5 days, the rats were decapitated. Cd, Zn and Cu levels were estimated in hepatic, renal, cardiac and skeletal muscle specimens. Cd was found to be significantly elevated in all tissues (p< 0.001). Zn was increased in hepatic and renal tissues (p< 0.001 and p< 0.05) compared to the controls. Cu was also significantly increased in these tissues. Myocardial and skeletal muscle tissues also manifested a significant increase in Cu and Zn in this group. It is concluded that Cd administration alters the Zn and Cu status in vivo. However, the mechanism underlying the interactions between toxic and essential elements should be further investigated.

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Immunohistochemical Expression Of C-Erb-B2, Steroid Receptors And Cathepsin-D In Breast Carcinomas: A Clinicopathologic Trial, Hadi̇ce Eli̇f Peştereli̇, Okan Erdoğan, Bahar Kiliçarslan, Taner Çolak, Evren Tercan, Mustafa Akaydin, Şeyda Karaveli̇ Jan 2001

Immunohistochemical Expression Of C-Erb-B2, Steroid Receptors And Cathepsin-D In Breast Carcinomas: A Clinicopathologic Trial, Hadi̇ce Eli̇f Peştereli̇, Okan Erdoğan, Bahar Kiliçarslan, Taner Çolak, Evren Tercan, Mustafa Akaydin, Şeyda Karaveli̇

Turkish Journal of Medical Sciences

Aim: We investigated the role of c-erb-B2, hormone receptors and cathepsin D in breast carcinoma progression and their correlation with each other. Methods: Forty-six breast carcinoma patients, operated on in the deparment of surgery and diagnosed in the pathology department, were classified according to their histopathological characteristics; the results of immunostaining with estrogen and progesterone receptors, c-erb-B2 and cathepsin-D were correlated with the stage and the disease-free survival of the patients. Results: Nuclear positivity was observed in 23 (50 %) cases for estrogen receptor and in 16 (34.8 %) cases for progesterone receptor. There was membranous staining with c-erb-B2 in …

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The Short-Term Results Of Intensive Insulin Therapy In Preadolescent Children With Type-1 Diabetes, Ayşenur Ökten, Gülay Kaya, Mukaddes Doğan Kalyoncu, Gamze Çan Jan 2001

The Short-Term Results Of Intensive Insulin Therapy In Preadolescent Children With Type-1 Diabetes, Ayşenur Ökten, Gülay Kaya, Mukaddes Doğan Kalyoncu, Gamze Çan

Turkish Journal of Medical Sciences

Purpose: The aim of this study was to observe the effects of and compliance with an intensive insulin regimen in preadolescent children with type-1 diabetes. Patients and methods: Eleven insulin-dependent diabetic patients, five girls and six boys aged 8 - 11 years (mean 9 years 3 months) with a mean±SD diabetes duration of 2±1.07 years, participated in this study. The results of the intensive insulin regimen were evaluated after one-year follow-up in 11 patients, and in 8 patients at 18 months. In the first year of this study we aimed to bring about higher blood glucose than is generally advised, …

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Lung Functions In Workers Exposed To Tobacco Dust, Ahmet Tevfi̇k Sünter, Faruk Bağirici, Ci̇had Dündar, Abdullah Marangoz, Yildiz Pekşen Jan 2001

Lung Functions In Workers Exposed To Tobacco Dust, Ahmet Tevfi̇k Sünter, Faruk Bağirici, Ci̇had Dündar, Abdullah Marangoz, Yildiz Pekşen

Turkish Journal of Medical Sciences

Workers in the tobacco industry are exposed to tobacco dust, which has allergenic and irritative effects on airways. In this study, our aim was to determine whether tobacco dust has any effect on lung functions and volumes. A questionnaire about working conditions was applied to 448 workers, some of which had been exposed to tobacco dust while others had not, and all workers underwent measurements of spirometric flow and volume. Of the study group, 126 workers were exposed to tobacco dust for more than 10 years and 55 were not. All of these 181 workers were also nonsmokers. There were …

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Fetal 45,X/46,Xy Mosaicism, Cytogenetic And Morphologic Findings In Pregnancy, Murat Derbent, Banu Bi̇lezi̇kçi̇, İdi̇l Alpas, Volkan Baltaci Jan 2001

Fetal 45,X/46,Xy Mosaicism, Cytogenetic And Morphologic Findings In Pregnancy, Murat Derbent, Banu Bi̇lezi̇kçi̇, İdi̇l Alpas, Volkan Baltaci

Turkish Journal of Medical Sciences

No abstract provided.

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Irreversible Blindness: An Unusual Complication Of The Caldwell-Luc Operation, Gülhanim Haciyakupoğlu, Müsli̇me Yalaz, Altan Atakan Özcan, Erol Aksungur Jan 2001

Irreversible Blindness: An Unusual Complication Of The Caldwell-Luc Operation, Gülhanim Haciyakupoğlu, Müsli̇me Yalaz, Altan Atakan Özcan, Erol Aksungur

Turkish Journal of Medical Sciences

No abstract provided.

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A Case Of Malignant Hyperthermia During Sevoflurane Anesthesia, Si̇bel Bariş, Deni̇z Karakaya, Fuat Güldoğuş, Bi̇nnur Sarihasan, Ati̇lla Tekat Jan 2001

A Case Of Malignant Hyperthermia During Sevoflurane Anesthesia, Si̇bel Bariş, Deni̇z Karakaya, Fuat Güldoğuş, Bi̇nnur Sarihasan, Ati̇lla Tekat

Turkish Journal of Medical Sciences

No abstract provided.

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Hibernoma: A Case Report And Discussion Of A Rare Tumor, Mustafa Kösem, Meti̇n Karakök Jan 2001

Hibernoma: A Case Report And Discussion Of A Rare Tumor, Mustafa Kösem, Meti̇n Karakök

Turkish Journal of Medical Sciences

No abstract provided.

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Sialolithiasis (Salivary Stone), Mehmet Dalkiz, Necdet Doğan, Bedri̇ Beydemi̇r Jan 2001

Sialolithiasis (Salivary Stone), Mehmet Dalkiz, Necdet Doğan, Bedri̇ Beydemi̇r

Turkish Journal of Medical Sciences

No abstract provided.

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Central Chemosensitivity, Sleep, And Wakefulness, E. E. Nattie, A Li Jan 2001

Central Chemosensitivity, Sleep, And Wakefulness, E. E. Nattie, A Li

Dartmouth Scholarship

No abstract provided.

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Nasal Glioma: Report Of Two Cases, Ali̇ Kemal Uzunlar, Üstün Osma, Fahri̇ Yilmaz, İsmai̇l Topcu Jan 2001

Nasal Glioma: Report Of Two Cases, Ali̇ Kemal Uzunlar, Üstün Osma, Fahri̇ Yilmaz, İsmai̇l Topcu

Turkish Journal of Medical Sciences

No abstract provided.

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Is Eradication Of Helicobacter Pylori An Alternative Treatment For Rosacea?, Pinar Yüksel Başak, Buket Ci̇ci̇oğlu Aridoğan Jan 2001

Is Eradication Of Helicobacter Pylori An Alternative Treatment For Rosacea?, Pinar Yüksel Başak, Buket Ci̇ci̇oğlu Aridoğan

Turkish Journal of Medical Sciences

Several reports have focused on either the increased prevalence of Helicobacter pylori infection or lack of association in patients with rosacea. In this study, 19 patients with rosacea and 38 healthy controls were investigated for seropositivity to Helicobacter pylori by ELISA and the effects of eradication treatment on clinical improvement were monitored. Statistical analysis was performed using Student's t test, Mann-Whitney U test, Wilcoxon signed ranks test, McNemar's test, and chi-squared and correlation tests. No correlation was detected between the degree of severity of disease and Helicobacter pylori positivity. Our results suggest that Helicobacter pylori may be an aggravating factor, …

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Susceptibility Of Mycobacterium Tuberculosis Strains To First-Line And Second-Line Antituberculosis Drugs In Ege University Hospital, Candan Çi̇çek Saydam, Cengi̇z Çavuşoğlu, Di̇lek Burhanoğlu, Ni̇sel Özkalay, F. Zühre Badak, Altinay Bi̇lgi̇ç Jan 2001

Susceptibility Of Mycobacterium Tuberculosis Strains To First-Line And Second-Line Antituberculosis Drugs In Ege University Hospital, Candan Çi̇çek Saydam, Cengi̇z Çavuşoğlu, Di̇lek Burhanoğlu, Ni̇sel Özkalay, F. Zühre Badak, Altinay Bi̇lgi̇ç

Turkish Journal of Medical Sciences

Recently tuberculosis has shown a speedy worldwide spread. The incidence of drug-resistant Mycobacterium tuberculosis is increasing in almost all industrialized and developing countries. The epidemiology of multiple drug resistance varies in different regions and countries. The aim of this study was to determine the activities of first-line (isoniazid, rifampin, ethambutol and streptomycin) and second-line (kanamycin, para-aminosalicylic acid, ethionamide and capreomycin) antituberculosis drugs on 100 various clinical isolates of M. tuberculosis. Mycobacterium tuberculosis ATCC 27294, ATCC 35838, ATCC 35825 and ATCC 35837 were used for internal quality control. First-line drug resistant strains were isolated from 10 clinical specimens. Six of them …

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Bcl - 2 Expression In Carcinoma Of The Uterine Cervix And Its Relationship With Prognostic Variables, Sema Zergeroğlu, Tayfun Güngör, Orhan S. Aksakal, Emel Ebru Parlakyi̇ği̇t, Oya Gökmen Jan 2001

Bcl - 2 Expression In Carcinoma Of The Uterine Cervix And Its Relationship With Prognostic Variables, Sema Zergeroğlu, Tayfun Güngör, Orhan S. Aksakal, Emel Ebru Parlakyi̇ği̇t, Oya Gökmen

Turkish Journal of Medical Sciences

Objective: The aim of this study was to evaluate the importance of the apoptotic activity of squamous cell carcinoma of the cervix and Bcl-2 expression. Materials and Methods: Twenty-seven women with cervical squamous cell carcinoma, admitted to our unit between 1996 and 1998, were reviewed. Patients were classified according to staging and grading systems. Bcl-2 protein material was investigated by immunohistochemistry (streptoavidin, biotine, alkaline phosphatase technique). Results: After immunohistochemical staining, 12 of the 27 cases stained positively (44%). There was a correlation between the grade and staining percentage. We found no significant relationship with stage and staining intensity. Conclusions: Bcl-2 …

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Homocysteine Levels And Other Risk Factors In Coronary Heart Disease, Gi̇ray Bozkaya, Aydan Çavuşoğlu, Si̇bel Bi̇lgi̇li̇, Baysal Karaca, Ercüment Cengi̇z, Oktay Bi̇lgi̇r Jan 2001

Homocysteine Levels And Other Risk Factors In Coronary Heart Disease, Gi̇ray Bozkaya, Aydan Çavuşoğlu, Si̇bel Bi̇lgi̇li̇, Baysal Karaca, Ercüment Cengi̇z, Oktay Bi̇lgi̇r

Turkish Journal of Medical Sciences

Many risk factors for coronary heart disease (CHD) have been reported to date. Recently, it has been shown that elevations in homocysteine (Hcy) levels may contribute to the development of coronary heart diseases. In this study, our aim was to determine the relation of Hcy levels and other risk factors in CHD. Serum Hcy, lipoprotein(a) [Lp(a)], C-reactive protein (CRP), fibrinogen, cholesterol, triglyceride, HDL and LDL levels were measured in 51 male patients, diagnosed with CHD with negative T elevations in electrocardiograms, and in 20 healthy males. Hcy was determined in serum samples with Fluorescence Polarization Immunoassay (FPIA) on Abbott IMx. …

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Faculty Publications 2000, Lsu Health Sciences Center New Orleans Jan 2001

Faculty Publications 2000, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 824 publications.

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Ups And Downs Of Mucosal Cellular Immunity Against Protozoan Parasites, Lloyd H. Kasper, Dominique Buzoni-Gatel Jan 2001

Ups And Downs Of Mucosal Cellular Immunity Against Protozoan Parasites, Lloyd H. Kasper, Dominique Buzoni-Gatel

Dartmouth Scholarship

No abstract provided.

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Titrating And Evaluating Multiple Drug Regimens Within Subjects, Margaret Shih Jan 2001

Titrating And Evaluating Multiple Drug Regimens Within Subjects, Margaret Shih

Theses and Dissertations

The dosing of combination therapies is commonly undertaken empirically by practicing physicians, and there is a lack of a coherent algorithm to approach the problem of combination dosing. Current methods of evaluating multiple drug combinations in clinical trials generally do not provide information regarding the location of more effective dosages when the combination is not found to differ from the standard, even though the absence of a difference does not necessarily mean the new combination is ineffective. Additionally, if a new combination is found to be more effective, often a large proportion of the subjects has not benefited from the …

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Epstein-Barr Virus Serology In The Diagnosis Of Nasopharyngeal Carcinoma From Sarawak, Madeline Wong Mei Fong Jan 2001

Epstein-Barr Virus Serology In The Diagnosis Of Nasopharyngeal Carcinoma From Sarawak, Madeline Wong Mei Fong

Student Works (2000-2009)

The Epstein-Barr virus (EBV) is closely associated with nasopharyngcal carcinoma (NPC). The role(s) of EBV in NPC remains unclear but antibodies to two EBV proteins namely the viral capsid antigen (VCA) and early antigen (EA), are clinically useful diagnostic markers of NPC. The NPC scenario in Malaysia has been based on studies done on West Malaysian patients but not much has been described for NPC in Sarawak, East Malaysia. In this study, 164 NPC sera from newly histologically confirmed NPC patients were collected from the Radiotherapy Unit of Kuching General Hospital, Sarawak prior to radiotherapy. One hundred and forty seven …

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Microbial Biofilms: From Ecology To Molecular Genetics, Mary Ellen Davey, George A. O'Toole Dec 2000

Microbial Biofilms: From Ecology To Molecular Genetics, Mary Ellen Davey, George A. O'Toole

Dartmouth Scholarship

Biofilms are complex communities of microorganisms attached to surfaces or associated with interfaces. Despite the focus of modern microbiology research on pure culture, planktonic (free-swimming) bacteria, it is now widely recognized that most bacteria found in natural, clinical, and industrial settings persist in association with surfaces. Furthermore, these microbial communities are often composed of multiple species that interact with each other and their environment. The determination of biofilm architecture, particularly the spatial arrangement of microcolonies (clusters of cells) relative to one another, has profound implications for the function of these complex communities. Numerous new experimental approaches and methodologies have been …

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Technology Evaluation: Pro-542, Progenics Pharmaceuticals Inc., Muhammad Mukhtar, Zahida Parveen, Roger J Pomerantz Dec 2000

Technology Evaluation: Pro-542, Progenics Pharmaceuticals Inc., Muhammad Mukhtar, Zahida Parveen, Roger J Pomerantz

Department of Medicine Faculty Papers

Progenics's rCD4-IgG2 (PRO-542) is a recombinant fusion protein, which has been developed using the company's Universal Antiviral Binding (UnAB) technology, and is in phase I/II clinical trials for the treatment of human immunodeficiency virus type I (HIV-1) infection [273391]. At the beginning of 1997, Progenics received a Phase II Small Business Innovation Research Program (SBIR) grant from the National Institute of Allergy and Infectious diseases (NIAID) to fund the development of PRO-542 [236048]. A further grant of $2.7 million was awarded in August 1998 for the clinical evaluation of PRO-542 and other anti-HIV therapies [294200]. Progenics is collaborating with the …

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Glucocorticoid Regulation Of Insulin Like Growth Factor Binding Protein-5 Gene Transcription In Human Osteoblasts, Xiaoying Wang Dec 2000

Glucocorticoid Regulation Of Insulin Like Growth Factor Binding Protein-5 Gene Transcription In Human Osteoblasts, Xiaoying Wang

Loma Linda University Electronic Theses, Dissertations & Projects

Glucocorticoids (GCs) inhibit bone formation in vivo and inhibit osteoblast proliferation and collagen synthesis in vitro. These effects may be mediated by alterations in the insulin-like growth factor (IGF) system. In the present study of normal human osteoblast-like (HOB) cells, we tested the hypothesis that dexamethasone (Dex) inhibits the expression of IGF binding protein-5 (IGFBP-5). Dex decreased IGFBP-5 mRNA levels to 54% of control after 4 hr. Dex did not modify the decay of IGFBP-5 mRNA in transcriptionally arrested osteoblast cells. Dex decreased IGFBP-5 hnRNA levels to 67% of control after 2 hr, and the activity of the human IGFBP- …

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Attachment Of Toxoplasma Gondii To A Specific Membrane Fraction Of Cho Cells, Chaitali Dutta, Jane Grimwood, Lloyd H. Kasper Dec 2000

Attachment Of Toxoplasma Gondii To A Specific Membrane Fraction Of Cho Cells, Chaitali Dutta, Jane Grimwood, Lloyd H. Kasper

Dartmouth Scholarship

We have observed previously that attachment of Toxoplasma gondii to synchronized host cells is considerably increased at the mid-S phase (4 h postrelease). Synchronized CHO host cells at the mid-S phase were fractionated by molecular weight, and the antigens were used to produce a panel of polyclonal mouse antisera. The polyclonal antisera raised against fraction 4 with molecular mass ranging approximately from 18 to 40 kDa significantly reduced attachment to mid-S-phase host cells. Immunofluorescence assays demonstrated strong reactivity to mid-S-phase host cells and identified a number of potential receptors on Western blots. These data indicate that there is a specific …

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