Digital Commons Network^™

A Comparison Of Three High Performance Liquid Chromatographic (Hplc) Methods For Measurement Of Plasma Total Homocysteine, Emi̇n Özgür Akgül, Erdi̇nç Çakir, Ömer Özcan, Hali̇l Yaman, Cumhur Bi̇lgi̇, Mehmet Kemal Erbi̇l

Turkish Journal of Medical Sciences

Homocysteine (tHcy) has been in routine use in many centers after this amino acid was reported as an independent risk factor for cardiovascular, cerebrovascular and peripheral vascular diseases. Many high-performance liquid chromatographic (HPLC) methods including a commercially available kit (Chromsystems) were reported for measuring tHcy in biologic samples. Although the commercial kits are believed to have higher precision, sensitivity and accuracy as well as practical and fast, it may cause high prices by increased test ordering. In this study we aimed to compare two different low-cost methods which had similar advantages with commercial kit as a reference method. In the …

Salivary Malondialdehyde Levels In Patients With Oral Leukoplakia, Yegane Güven, Meral Ünür, Kivanç Bektaş, Ezel Uslu, Ahmet Belce, Erdi̇nç Demi̇rez, Şule Can

Turkish Journal of Medical Sciences

Since leukoplakia is a premalignant condition, it is evident that an effective means of control would contribute to reducing the mortality and morbidity of oral cancer. The reactivity of free radicals and singlet oxygen is believed to be an important factor in the pathogenesis of many degenerative diseases including carcinogenesis. Reactive free radicals may damage cells by induction of lipid peroxidation of the polyunsaturated fatty acids. Buege and Aust's method was used for lipid peroxidation analysis. One way of estimating free radical activity and lipid peroxidation is to determine the concentration of malondialdehyde (MDA) in the body fluids. MDA concentration …

Determination Of Compressibility And In Vitro Release Properties Of Acetaminophen Granules Coated With Eudragit E 30 D, Tansel Çomoğlu, Ahmet Aydinli, Tamer Baykara

Turkish Journal of Medical Sciences

OMOĞLU, Ahmet AYDINLI, Tamer BAYKARA Department of Pharmacutical, Faculty of Pharmacy, Ankara University, 06100 Tandoğan, Ankara - Turkey

Amputated Tuba-Ovarian Torsion In A Newborn, Mi̇ne Özkol, Gülgün Yilmaz Ovali, Özüm Tunçyürek, Aydin Şencan, Yüksel Pabuşçu

Turkish Journal of Medical Sciences

OL^1, Gülgün Yılmaz OVALI^1, Özüm TUNÇYÜREK^1, Aydın ŞENCAN^2, Yüksel PABUŞÇU^1 ^1Department of Radiology, Faculty of Medicine, Celal Bayar University, Manisa - Turkey ^2Department of Pediatric Surgery, Faculty of Medicine, Celal Bayar University, Manisa - Turkey

Megadose Methylprednisolone For Childhood Idiopathic Trombocytopenic Purpura (Itp), Şi̇nasi̇ Özsoylu

Turkish Journal of Medical Sciences

Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder. Decreased platelet survival is the main pathogenesis, which is related to platelet antibodies (APA). The levels of these antibodies are correlated with relapse and remission which was shown for the first time by our group. Although APA levels decrease in remission they do not disappear as we have shown. Among the several appraches to ITP treatment, oral megadose methylprednisolone is found to be the cheapest and most effective one. For oral MDMP treatment, admission of the patients is not required and there is some evidence that chronic ITP could be prevented by …

Pharmacotherapy In Cardiopulmonary Resuscitation (Cpr), Berri̇n Günaydin

Turkish Journal of Medical Sciences

Cardiac arrest is defined as cessation of cardiac mechanical activity. Cardiopulmonary resuscitation (CPR) is an attempt to restore spontaneous circulation through several maneuvers and techniques. Although the two interventions, which are competent basic life support and prompt defibrillation, improve the survival rate, several adjuvant cardiac medication drugs are advocated to treat cardiac arrest during advanced cardiac life support. Since the introduction of modern CPR there have been many advances in the field of pharmacotherapy in CPR. Therefore, we aimed to summarise the cardiac medication drugs used for patients in ventricular fibrillation (VF) or pulseless ventricular tachycardia (VT) or non VF/VT …

Effects Of Ace Inhibition And At1 Receptor Blockade On Cardiac Ischaemia-Reperfusion Induced Mortality And Cardiac Markers In Rats, Mustafa Iraz, Şemsetti̇n Şahi̇n, Ercüment Ölmez, Ahmet Acet

Turkish Journal of Medical Sciences

Many studies have established the therapeutic benefits of angiotensin-converting enzyme (ACE) inhibitors such as reducing reperfusion arrhythmias, and angiotensin II type 1 (AT1) blocker may have similar effects to ACE inhibitors. In this study, it was aimed to compare the effects of an ACE inhibitor captopril and AT1 receptor blocker losartan on death from arrhythmias and biochemical markers such as cardiac troponin T and I (cTnT, cTnI), myoglobin, creatin kinase (CK), creatine kinase-MB isoenzyme (CK-MB) and aspartate aminotransferase (AST) after cardiac ischemia/reperfusion in an in vivo rat model. Study design and methods: sixty four male rats were divided into four …

Development Of Elisa Systems For Measurement Of Human Tumor Necrosis Factor-Alpha (Tnf-\Alpha), Ayşegül Yücel, Cemaletti̇n Aybay

Turkish Journal of Medical Sciences

The aim of this study was to develop a human tumor necrosis factor alpha (hTNF-\alpha) ELISA system because specific and sensitive measurement of low levels of circulating TNF-a is very important for enlightening the immunopathologial mechanisms associated with TNF-\alpha. Monoclonal antibodies 6A4c and 8A6 were produced against hTNF-\alpha and were used as the capture antibody and the tracer antibody respectively for the first hTNF-\alpha ELISA system (6A4c/biotin-8A6). Murine polyclonal IgG was used as the tracer antibody in the second hTNF-\alpha ELISA system (6A4c/biotin-polyclonal IgG). Both systems could detect both recombinant hTNF-\alpha and native hTNF-\alpha. The detection limits defined as minimal …

Sjögren's Syndrome: A Study Of Salivary Electrophoresis, Mehmet Yaltirik, Hülya Koçak Berberoğlu, Kivanç Ergen, Bariş Aydil

Turkish Journal of Medical Sciences

Many diagnostic tests exist to assess the salivary and lacrimal involvement in Sjögren's syndrome (SS), but there is still disagreement. The purpose of this study was to examine salivary proteins in patients with primary Sjögren's syndrome and healthy control subjects by the polyacrylamide gel electrophoresis, as well as the applicability of the method. The polyacrylamide gel electrophoresis, applied in our examinations, is a simple and rapid method, requiring only a minute amount of saliva for the analysis. Saliva were collected from patients suffering from primary Sjögren's syndrome (n = 10) and a control group (n = 10). All saliva samples …

Palm Is Expressed In Both Developing And Adult Mouse Lens And Retina., Meryl Castellini, Louise V Wolf, Bharesh K Chauhan, Deni S Galileo, Manfred W Kilimann, Ales Cvekl, Melinda K Duncan

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Earlier studies have shown brain preferred Palm expression, although this protein is a major water insoluble protein in chicken lens fiber cells and the Palm gene may be regulated by Pax6. METHODS: The expression profile of Palm protein in the embryonic, newborn and adult mouse eye as well as dissociated retinal neurons was determined by confocal immunofluorescence. The relative mRNA levels of Palm, Palmdelphin (PalmD) and paralemmin2 (Palm2) in the lens and retina were determined by real time rt-PCR. RESULTS: In the …

A New Diagnostic System In Cancer Research: Bioluminescent Imaging (Bli), Z. Günnur Di̇kmen, Ginelle Gellert, Paki̇ze Doğan, Ralph Mason, Peter Antich, Edmond Richer, Woodring E. Wright, Jerry W. Shay

Turkish Journal of Medical Sciences

Bioluminescent imaging (BLI) is particularly well suited for imaging small animals, and it can be readily used by scientists who are already routinely using the luciferase gene as a reporter in cell-based assays. This strategy relies on ATP and an O_2 dependent photochemical reaction between luciferin and luciferase, resulting in the release of photons from only live cells. In BLI, the intensity of the light detected by the device is proportional to the intensity of light emitted and the relationship is quantitative. This study was performed to determine the sensitivity of BLI for the imaging of lung metastasis and breast …

The Quality Of Collaboration Among Medical Research Centers, Universities, Health Executives And The Community In İran, Saeed Asefzadeh

Turkish Journal of Medical Sciences

A health research system is defined as a system for planning, coordinating, monitoring and managing health research resources and activities, and for promoting research aimed at effective and equitable national health development. This study was conducted to describe the quality of mutual collaboration among medical research centers, universities, executive organizations and the community. In this situation analysis qualitative methods were applied. In-depth interviews were held with the heads of 20 research centers (RCs), 6 senior executive health administrators at national level, 10 vice chancellors at universities and 3 directors of private medical research firms. In addition to the interviews, records …

Prenatal Diagnosis Of A Trisomy 13 Case Associated With Holoprosencephaly By Ultrasonography And Quantitative Fluorescent Pcr, N. Lale Şatiroğlu Tufan, A. Çevi̇k Tufan, Başak Yildirim, Babür Kaleli̇, C. Nur Semerci̇, Ferda Bi̇r, Füsun Düzcan, Hüseyi̇n Bağci

Turkish Journal of Medical Sciences

ŞATIROĞLU TUFAN^{1,2}, A. Çevik TUFAN^{2,3}, Başak YILDIRIM^4, Babür KALELİ^4, C. Nur SEMERCİ^1, Ferda BİR^5, Füsun DÜZCAN^1, Hüseyin BAĞCI^{1,2} ^1Department of Medical Biology, Center for Genetic Diagnosis, Molecular Genetics Laboratory, Faculty of Medicine, Pamukkale University, Denizli - Turkey ^2Pamukkale University Research Center for Genetic Engineering and Biotechnology, Denizli - Turkey ^3Department of Histology and Embryology, Faculty of Medicine, Pamukkale University, Denizli - Turkey ^4Department of Obstetrics and Gynecology, Faculty of Medicine, Pamukkale University, Denizli - Turkey ^5Department of Pathology, Faculty of Medicine, Pamukkale University, Denizli - Turkey

A Compilation Of Homologous Cancer-Related Genes In Humans And Dogs, Julie Susan Brown

Honors Program Theses

The purpose of this study was to find out if spontaneously arising point mutations occurred in canine e-kit and if these mutations contributed to the development of mast cell tumors in dogs. Specimens were collected from 11 dogs after tumors had been surgically removed or the dog had died. The e-kit DNA was amplified using PCR, and the previously established point mutations were searched for (London et al., 1999). The point mutations found in human mast cell lines were not detected in the canine e-kit DNA, but new mutations were observed. These new mutations, observed in 50% of the tumors, …

The Pericentrion: Identification, Characterization, And Function Of A Novel Cpkc-Dependent Compartment, Kevin P. Becker

MUSC Theses and Dissertations

Members of the protein kinase C (PKC) superfamily transduce a myriad of transmembrane signals initiated by the formation of sn-l ,2-diacylglycerol (DAG). As the primary physiological agonist for C] domain containing PKC isoenzymes, DAG functions to regulate the spatial and temporal parameters of PKC activity. It is now recognized that cellular DAG levels can vary from minutes to hours and even days. These observations have suggested a division of PKC functions that correlate to the duration of the cellular DAG signal. In their classical role, cPKCs respond acutely to the short-lived (1-2 min) DAG generated at the plasma membrane by …

The Study Of The Effect Of Drugs Of Abuse On Protein Kinase A Activity In Mouse Brain And Spinal Cord, George D. Dalton

Theses and Dissertations

Morphine and Δ9-THC are drugs that produce analgesia and rewarding effects. However, chronic treatment with morphine and Δ9-THC produces problematic side-effects including tolerance and physical dependence. The cellular mechanisms underlying opioid and cannabinoid antinociceptive tolerance have been studied for years. Research has demonstrated that the expression of morphine and Δ9-THC antinociceptive tolerance may be mediated through intracellular signaling pathways, such as the adenylyl cyclase /Protein Kinase A (PKA) cascade. The present study investigated the role of PKA in the expression of morphine and Δ9-THC antinociceptive tolerance. Male Swiss Webster mice were treated chronically with morphine or Δ9-THC and the warm-water …

The Effect Of Antioxidant Supplementation On Human Sperm Cryopreservation, Katrina E. Taylor

Theses : Honours

Semen cryopreservation has an important role in assisted reproductive technology however, the cooling, freezing and thawing processes often result in a significant loss of sperm motility, viability and nuclear integrity. The destructive effects of cryopreservation are significantly exacerbated in samples exhibiting low sperm number and poor morphological characteristics. Recent research into infertility has focused on the correlation between excessive oxidation and subfertility, in particular radical induced lipid peroxidation within the phospholipid bilayer of the spermatozoon plasma membrane and the promotion of cellular damage as a result of antioxidant insufficiency. The aim of this study was to evaluate the effect of …

Mitochondrial Dna Mutations, Apoptosis, And The Misfolded Protein Response., Justin L. Mott, Dekui Zhang, Hans Peter Zassenhaus

Journal Articles: Biochemistry & Molecular Biology

Studies of transgenic mice with accelerated accumulation of mtDNA mutations specifically in the heart lead us to propose that apoptotic signaling and cell death is central to the pathogenesis of mtDNA mutations in aging. It is the cellular response to that apoptotic signaling and the organ?s compensatory response to a loss of cells that specify the phenotype of an accumulation of mtDNA mutations. In the heart, cardiomyocytes induce a vigorous anti-apoptotic, pro-survival response to counteract mitochondrial apoptotic signaling. The heart up-regulates contractility of remaining myocytes in order to maintain cardiac output. We hypothesize that mutant mitochondrial proteins originate apoptotic signaling …

The Use Of Time-Resolved Fluorescence Imaging In The Study Of Protein Kinase C Localisation In Cells., Christopher D Stubbs, Stanley W Botchway, Simon J Slater, Anthony W Parker

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: Two-photon-excitation fluorescence lifetime imaging (2P-FLIM) was used to investigate the association of protein kinase C alpha (PKCalpha) with caveolin in CHO cells. PKCalpha is found widely in the cytoplasm and nucleus in most cells. Upon activation, as a result of increased intracellular Ca2+ and production of DAG, through G-protein coupled-phospholipase C signalling, PKC translocates to a variety of regions in the cell where it phosphorylates and interacts with many signalling pathways. Due to its wide distribution, discerning a particular interaction from others within the cell is extremely difficult. RESULTS: Fluorescence energy transfer (FRET), between GFP-PKCalpha and DsRed-caveolin, was used …

Hinderin, A Five-Domains Protein Including Coiled-Coil Motifs That Binds To Smc3., Chirag A Patel, Giancarlo Ghiselli

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: The structural maintenance of chromosome proteins SMC1 and SMC3 play an important role in the maintenance of chromosomal integrity by preventing the premature separation of the sister chromatids at the onset of anaphase. The two proteins are constitutive components of the multimeric complex cohesin and form dimers by interacting at their central globular regions. RESULTS: In order to identify proteins that by binding to SMC3 may interfere with the protein dimerization process, a human cDNA library was screened by the yeast two-hybrid system by using the hinge region of SMC3 as bait. This has lead to the identification of …

The Evaluation Of Rectal Bleeding In Adults, Elizabeth Allen, Christina Nicolaidis, Mark Helfand

School of Social Work Faculty Publications and Presentations

Colon cancer is the third leading cause of cancer-related death in this country.5 The majority of medical societies recommend some form of colon cancer screening for asymptomatic adult patients over age 50. The evaluation of rectal bleeding is different from screening because the risk of serious disease is higher and it is unclear whether early diagnosis and treatment of serious disease results in improved mortality once gross bleeding has occurred. The optimal evaluation strategy for rectal bleeding is unknown. Neither historical information nor the presence or absence of hemorrhoids has been shown to reliably differentiate benign from serious disease.6–11 Fecal …

Global Gene Expression Profiling Of Cells Overexpressing Smc3., Giancarlo Ghiselli, Chang-Gong Liu

Department of Microbiology and Immunology Faculty Papers

BACKGROUND: The Structural Maintenance of Chromosome 3 protein (SMC3) plays an essential role during the sister chromatid separation, is involved in DNA repair and recombination and participates in microtubule-mediated intracellular transport. SMC3 is frequently elevated in human colon carcinoma and overexpression of the protein transforms murine NIH3T3 fibroblasts. In order to gain insight into the mechanism of SMC3-mediated tumorigenesis a gene expression profiling was performed on human 293 cells line stably overexpressing SMC3. RESULTS: Biotinylated complementary RNA (cRNA) was used for hybridization of a cDNAmicroarray chip harboring 18,861 65-mer oligos derived from the published dEST sequences. After filtering, the hybridization …

Association Between Coinfection Of Porphyromonas Gingivalis, Actinobacillus Actinomycetemcomitans And Treponema Denticola And Periodontal Tissue Destruction In Chronic Periodontitis, L. L. Chen, Y. M. Wu, J. Yan, W. L. Sun, Y. Z. Sun, David M. Ojcius

All Dugoni School of Dentistry Faculty Articles

No abstract provided.

Replication And Encapsidation Of Papillomaviruses In Saccharomyces Cerevisiae, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Improvements in methodologies to recapitulate and study particular biological functions of the

papillomavirus life cycle have led to great advances in our knowledge of these viruses. Described in

this chapter are techniques that allow low-copy and high-copy replication of full-length human

papillomavirus (HPV) genomes, as well as assembly of virus-like particles, in Saccharomyces

cerevisiae (yeast). This system has several distinct advantages that make it an attractive complement

to the well-established raft-culturing system. First, yeast are inexpensive, rapid, and simple to culture

in the lab. Second, they provide an ever-widening array of genetic tools to analyze HPV functions

—most recently notable, …

Antigenicity And Immunogenicity Of A Synthetic Human Immunodeficiency Virus Type 1 Group M Consensus Envelope Glycoprotein, Feng Gao, Eric A. Weaver, Zhongjing Lu, Yingying Li, Hua-Xin Liao, Benjiang Ma, S. Munir Alam, Richard M. Scearce, Laura L. Sutherland, Jae-Sung Yu, Julie M. Decker, George M. Shaw, David C. Montefiori, Bette T. Korber, Beatrice H. Hahn, Barton F. Haynes

Nebraska Center for Virology: Faculty Publications

Genetic variation of human immunodeficiency virus (HIV-1) represents a major obstacle for AIDS vaccine development. To decrease the genetic distances between candidate immunogens and field virus strains, we have designed and synthesized an artificial group M consensus env gene (CON6 gene) to be equidistant from contemporary HIV-1 subtypes and recombinants. This novel envelope gene expresses a glycoprotein that binds soluble CD4, utilizes CCR5 but not CXCR4 as a coreceptor, and mediates HIV-1 entry. Key linear, conformational, and glycan-dependent monoclonal antibody epitopes are preserved in CON6, and the glycoprotein is recognized equally well by sera from individuals infected with different HIV-1 …

Antigenicity And Immunogenicity Of A Synthetic Human Immunodeficiency Virus Type 1 Group M Consensus Envelope Glycoprotein, Feng Gao, Eric A. Weaver, Zhongjing Lu, Yingying Li, Hua-Xin Liao, Benjiang Ma, S. Munir Alam, Richard M. Scearce, Laura L. Sutherland, Jae-Sung Yu, Julie M. Decker, George M. Shaw, David C. Montefiori, Bette T. Korber, Beatrice H. Hahn, Barton F. Haynes

Nebraska Center for Virology: Faculty Publications

Genetic variation of human immunodeficiency virus (HIV-1) represents a major obstacle for AIDS vaccine development. To decrease the genetic distances between candidate immunogens and field virus strains, we have designed and synthesized an artificial group M consensus env gene (CON6 gene) to be equidistant from contemporary HIV-1 subtypes and recombinants. This novel envelope gene expresses a glycoprotein that binds soluble CD4, utilizes CCR5 but not CXCR4 as a coreceptor, and mediates HIV-1 entry. Key linear, conformational, and glycan-dependent monoclonal antibody epitopes are preserved in CON6, and the glycoprotein is recognized equally well by sera from individuals infected with different HIV-1 …

Role Of The Hypervariable Hinge Region Of Phosphoprotein P Of Vesicular Stomatitis Virus In Viral Rna Synthesis And Assembly Of Infectious Virus Particles, Subash C. Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P protein) of vesicular stomatitis virus (VSV) is an essential subunit of the viral RNAdependent RNA polymerase and has multiple functions residing in its different domains. In the present study, we examined the role of the hypervariable hinge region of P protein in viral RNA synthesis and recovery of infectious VSV by using transposon-mediated insertion mutagenesis and deletion mutagenesis. We observed that insertions of 19-amino-acid linker sequences at various positions within this region affected replication and transcription functions of the P protein to various degrees. Interestingly, one insertion mutant was completely defective in both transcription and replication. Using …

Periplasm Organization In Treponema Denticola As Studied By Cryo-Electron Tomography, Jacques Izard, C.-E. Hsieh, C. A. Mannella, Ronald J. Limberger, M. Marko

Department of Food Science and Technology: Faculty Publications

As a spirochete, the genus Treponema is one of the few major bacterial groups whose natural phylogenic relationships are evident at the level of gross phenotypic characteristics such as their morphology. Treponema spp. are highly invasive due to their unique motility in dense media, and their ability to penetrate cell layers. This feature is associated with the helical cell body and the presence of flagellar filaments in the periplasm. Treponema denticola is an oral pathogen involved in endodontic infections and periodontal diseases. The presence and quantity of T. denticola in the subgingival biofilm is correlated with the severity of periodontal …

Lipid Profile And Lipid Peroxidation Pattern Pre And Post Exercise In Coronary Artery Disease, Hedef D. El-Yassin, Nabil M. A. Hasso, Hassan A. Al-Rubayi

Turkish Journal of Medical Sciences

Increased aerobic metabolism during exercise is a potential source of oxidative stress, which plays an important role in the development of atherosclerotic disease, while antioxidants delay or prevent various steps in atherosclerosis. The aim of this study was to identify the effect of oxidative stress induced by the TMT (Treadmill exercise Test) and the lipid peroxidation level as and lipid profile in blood, which may help in confirming the diagnosis of CAD (Coronary Artery Disease) in borderline cases of TMT results. This may help in future for early detection and possible prevention of atherosclerosis especially in people with a family …

Lymphocyte Subpopulations In Patients With Acute Brucellosis, İlhami̇ Çeli̇k, H. Handan Akbulut

Turkish Journal of Medical Sciences

The aim of this work was to evaluate changes in lymphocyte subpopulations, especially helper and cytotoxic T cells, in acute brucellosis patients undergoing treatment. Forty-three acute brucellosis patients were included in the study. Twenty healthy subjects served as controls. Total lymphocytes and the CD3^+, CD4^+, CD8^+, CD19^+ and CD (16+56)^+ subpopulations were counted by two-color flow cytometric analysis. The CD4+ counts in patients before and after treatment were not statistically different (p = 0.7), but healthy subjects had significantly more of these cells (p = 0.001 and p = 0.001 compared to pre- and post-treatment patients, respectively). The CD8^+ counts …