Digital Commons Network^™

Role Of Cytochrome P-450 In Methemoglobin Formation By Primaquine With Hamster Liver Microsomes, Tommy L. Hicks

Loma Linda University Electronic Theses, Dissertations & Projects

The role of cytochrome P-450 in in vitro methemoglobin formation by primaquine was investigated through a series of experiments with golden-Syrian hamsters, treated before sacrificing with phenobarbital or 20-methylcholanthrene to increase the cytochrome P-450 content or with cobaltous chloride to cause its decrease. To determine the extent of induction in the liver microsomal cytochrome P-450-dependent mixed-function oxidases, cytochrome P-450 content and formaldehyde from the 0-demethylation of p-nitroanisole were measured. A 90% increase occurred in cytochrome P-450 content with phenobarbital pretreatment and an increase of 102% was observed in p-nitroanisole 0-demethylation. Formaldehyde from primaquine increased 64%. The methylcholanthrene treatment produced a …

Computer Assisted Electron Spin Resonance Spectral Analysis Of Trapped Radicals, Philip Mook Wah Law

Loma Linda University Electronic Theses, Dissertations & Projects

A set of general purpose FORTRAN IV programs have been written during this study to assist in the analysis of complex spectra. An interactive approach is adopted, utilizing the computer and graphics terminals of the Scientific Computation Facility at Loma Linda University. Some procedures carried out are inversion, coordinates scaling, smoothing, differentiation and integration, correction for slanting baseline, addition and subtraction, and spectral simulation.

As an application, the programs are used to assist in the study of free radicals in gamma-irradiated single crystals of 1,2,3,- benzene tricarboxylic acid dihydrate by electron spin resonance spectroscopy. Is is found that the predominant …

Stereospecific Opiate Binding In Human Erythrocyte Membranes And Changes In Heroin Addicts, Leo G. Abood, Holly G. Atkinson, Mary Macneil

Publications and Research

Stereospecific opiate binding has been demonstrated in human erythrocyte membranes, having a Kd of 9-10(-9) M. In most respects the binding characteristics resemble those of synaptic membranes. These included the correlation of binding affinity and pharmacological potency of opiates; competition by naloxone; inhibition by Ca2+ and Na+; and sensitivity to phospholipases and trypsin. A comparison of stereospecific opiate binding in control human subjects and heroin addicts revealed a 43% increase in the addict group.

Ultrastructure And Chemical Composition Of Microconidial Walls Of Trichophyton Mentagrophytes, Christine Da-Ruh Wu

Dissertations

No abstract provided.

Patterns Of Fatty Acid Release From Endogenous Substrates By Human Platelet Homogenates And Membranes

Faculty, Staff and Students Publications

We describe a method for measuring the release of fatty acids from endogenous substrates of human platelet homogenates and membranes. The method depends on the availability of lipids whose fatty acids are odd-chained and therefore suitable as internal reference compounds that, at the time of lipid extraction, can be added to an incubation to permit subsequent quantification of the content of free fatty acids or fatty acids esterified to specific lipids. We found four types of lipolytic activities in human platelets. In homogenates at pH 4.0 a triglyceride lipase operated as shown by the synchrony of triglyceride degradation and release …

Atomic Models For The Polypeptide Backbones Of Myohemerythrin And Hemerythrin

Faculty, Staff and Students Publications

Background: The novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread rapidly after its identification in December 2019 to cause a global pandemic. The respiratory tract is the primary site of infection, and there is a large range in the severity of respiratory illnesses caused by the virus. Defining molecular and cellular factors for protection from severe disease and death has been a goal to better understand and to predict and mitigate the effects of SARS-CoV-2 and future coronaviruses.

Objective: Despite well-known susceptibilities to respiratory viral infections, respiratory allergy and allergic asthma have not been identified as risk factors …

An Analysis Of Blood Cortisol Levels In Oral Surgery Patients Under Various Levels Of Intravenous Medication, Fred L. Bunch

Loma Linda University Electronic Theses, Dissertations & Projects

This study was designed to objectively evaluate each pharmacological component of the Loma Linda intravenous sedation technique. The results were evaluated as a function of stress as measured by plasma cortisol levels.

The study was conducted on fifty patients in groups of ten. Blood samples were taken at set procedure intervals during dental extraction of third molar teeth. A component of the Loma Linda technique was deleted from each of the groups to ascertain its role in sedation and relief of stress.

Results show a trend toward reduction of plasma cortisol levels as the procedure progresses, indicating a measure of …

The Effect Of A Pufa Diet On Human Red Blood Cell Levels Of 2, 3-Dpg, Sandra A. Dassenko

Loma Linda University Electronic Theses, Dissertations & Projects

Fasting blood was collected at weekly intervals and the level of 2,3-DPG of the red blood cell was determined in 15 male subjects age 28 to 57 with serum cholesterol levels greater than 250 mg%. A lactovegetarian diet containing no cholesterol, a high level of dietary fiber and a P/S ratio of 4.0 was provided for 28 days.

During the course of the experimental period the 2,3-DPG increased by an average of 0.83> «moles/ml red blood cell which was highly significant (p^.001). This significant increase of 2,3-DPG which relates to an increased oxygen delivery capability of the red blood cells …

Bibliography Of Secondary Sources On The History Of Dermatology Ii. Obituaries And Biographies In English Before 1973, Lawrence Charles Parish, M.D., John Thorne Crissey, M.D., Jennifer L. Parish, M.D., Daniel H. Parish, J.D.

Department of Dermatology and Cutaneous Biology Faculty Papers

Bibliography of Secondary Sources on the History of Dermatology

II. Obituaries and Biographies in English before 1973

A Descriptive Study Of Women's Attitudes Towards Obtaining Or Not Obtaining Papanicolaou Smears, Linda J. Shults

Loma Linda University Electronic Theses, Dissertations & Projects

It was the purpose of this study to identify the attitudes of a selected group of women which influenced their participation in Papanicolaou testing. These attitudes were inferred from the reasons women gave for obtaining the test. The subjects were randomly selected from the telephone book of a medium-sized town in Southern California. The 135 women who participated in the telephone interview were considered to be the respondents in the study.

A review of the literature revealed that through the annual use of the Papanicolaou smear, approximately 11,000 deaths every year from uterine and cervical cancer could be prevented, Attitudes …

An Evaluation Of The Cytotoxicity Of Root Canal Filling Materials On Tissue Culture Cells In Vitro: Grossman's Sealer, N2 Permanent, Rickert's Sealer, And Cavit, Donald D. Antrim

Loma Linda University Electronic Theses, Dissertations & Projects

An in vitro study using radioactively-labeled tissue culture cells was conducted over a seven-month period on Grossman's sealer, Rickert's sealer, N₂ Permanent, and Cavit to determine the lasting and relative tissue toxicity of these materials.

From this study it was observed that all materials possess lasting tissue toxicity. When relative toxicity is considered, Grossman's is the most toxic, followed by N₂, Rickert's and Cavit in descending order of toxicity. Both Grossman's and N₂ would be considered highly toxic, with Rickert's displaying moderate toxicity. Cavit would be considered mild to moderate in toxicity.

The use of radioactively-labeled …

The Relationship Of Alcohol Consumption In Rats To Morphine And Codeine Intake And The Metabolism Of Dopamine, Hsiu Fen Liu

Loma Linda University Electronic Theses, Dissertations & Projects

The effects of morphine and codeine free-choice alcohol consumption in male Sprague-Dawley rats were studied. Intakes of a 10% alcohol solution in milliliters per 100 gm. of body weight were on recorded weekly. No difference was found in the alcohol intake of rats given either a morphine-supplemented diet (0.01% of the diet) or daily injection of morphine sulfate (2 mg./kg.); however, there was a significant lower intake of water (p < .05) in those rats fed the diet with morphine. Total fluid consumption of both groups given the morphine-supplemented diet and daily injection of morphine sulfate was decreased (p < .05). It was found that morphine did not affect the percentage of total fluid consumed as alcohol or the weight gain.

Two levels of codeine phosphate (10 mg./kg. and 20 mg./kg.) were injected subcutaneously into rats twice a day. It was observed that the codeine significantly decreased alcohol intake in rats …

The Effect Of Diet On Neutrophilic Phagocytosis, Linda L. Hoover

Loma Linda University Electronic Theses, Dissertations & Projects

Two series of experiments were conducted in order to determine the effects of diet on neutrophilic phagocytosis. The first series was designed to determine the effects of varying amounts of oral glucose on phagocytosis. Blood samples from 6 to 8 subjects were drawn at fasting, and at various time periods following ingestion of 12.5, 25, 50, 75, and 100 grams of glucose. In the second series of experiments, blood samples were taken from 11 subjects after an overnight fast and 2 hours following ingestion of one of 4 different liquid diets, which included the following: the meal (I), consisting of …

Acid-Base Disorders: Evaluation And Instruction By Minicomputer, Deborah Likins Atkinson

MUSC Theses and Dissertations

A computerized system for instruction and diagnosis of clinical electrolyte and acid-base disorders has been implemented on a PDP-8/e minicomputer at the Medical University of South Carolina. The program is designed for use by persons at various stages of medical training: student, house staff, and clinician. This system not only aids the physician in making his diagnosis, but also serves as a means of educational instruction in acid-base physiology for the student. A patient file has been established in conjunction with the program. The file enables a physician to follow a patient's course over time. Several auxiliary computer programs have …

Seasonal And Spatial Distribution Of The Plankton Of Barren Lake, Kentucky, With Special Reference To The Phytoplankton Community, John Wright

Masters Theses & Specialist Projects

A study of the seasonal and spatial distribution of phytoplankters in relation to physio-chemical parameters and discharge regime was conducted in Barren Lake, Kentucky, a flood control reservoir with a dam equipped with a multilevel outlet, from February, 1970, through January, 1971. Paired samples were collected every two weeks from April through October and every three weeks from November through March. Samples were obtained from eight depths in the main pool area of the reservoir and from the tailwater. Reservoir discharge was primarily from the upper 3 m from April through September. At other times, discharge incorporated lower levels to …

Value Of Oxygen With Innovar And Methohexital For Outpatient Oral Surgery, Andrew D. Harsany

Loma Linda University Electronic Theses, Dissertations & Projects

This paper demonstrates the value of oxygen in maintaining arterial blood gas in an adequate range during outpatient oral surgery. Dental extractions were completed on ten patients under an anesthetic technique utilizing Innovar and methohexital with supplemental nasal oxygen. Blood gas samples were drawn at five stages during the procedure.

The mean of the PaO₂ samples drawn with the patient at the point of deepest sedation was approximately three times the normal value.

Oxygen for pulmonary ventilation provides a reservoir in the lungs, saturation of hemoglobin, and increased oxygen in the plasma which provides a margin of safety if …

The Effect Of Glucose On The Food Intake Of Goldthioglucose Injected Mice, Janet Lahti

Dissertations and Theses

Jean Mayer has postulated the presence of glucoreceptors located primarily in the ventromedial hypothalamic nuclei of the brain which are activated by glucose in the measure that they utilize it. According to Mayer, hunger occurs when glucose is not available for metabolic purposes and satiation supervenes when glucose is made available. Control of this mechanism rests with the glucoreceptors. These receptors are believed to be destroyed when an injection of the compound goldthioglucose is given to mice. These animals subsequently become obese and are found to have gold deposits and lesions in the ventromedial area and also in other parts …

Mathematical Model Of Placental Oxygen And Carbon Dioxide Exchange, Ester P. Hill

Loma Linda University Electronic Theses, Dissertations & Projects

This model of placental CO₂ and O₂ exchange calculates the time course of changes in P_^CO2, [HCO₃^-], and [H⁺] in each of four "compartments" of placental exchange vessels: maternal erythrocytes, maternal plasma, fetal plasma and fetal erythrocytes. The changes in hemoglobin-bound CO₂ (carbamate) and oxyhemoglobin contents within the erythrocytes are also calculated, permitting consideration of the Haldane and Bohr effects. Equations were written to represent one-dimensional steady state CO₂ and O₂ diffusion between concurrent maternal and fetal capillaries and the subsequent hydration, carbamino and oxyhemoglobin reactions. The equations …

Effect Of Lateral Positioning On Central Venous Pressure, Romelda C. Jereos

Loma Linda University Electronic Theses, Dissertations & Projects

This experimental study was conducted to investigate the effects of lateral positioning on central venous pressure. It was hypothesized that lateral positioning would not significantly change the central venous pressure so long as the subjects were placed on the same horizontal plane for both supine and lateral measurements. It was also hypothesized that the length of time spent in the lateral position would not alter the pressure.

It is recognized that several factors do indeed play important roles in the maintenance of central venous pressure. The effect of one particular influencing factor, gravity, was central to this study. The difference …

The Duration Of The Effect Of Different Carbohydrates On Neutrophilic Phagocytosis, Judy L. Reeser

Loma Linda University Electronic Theses, Dissertations & Projects

In order to determine the duration of the effect of glucose, fructose, sucrose, honey, starch, and orange juice on neutrophilic phagocytosis, 10 subjects underwent a complete series of carbohydrate tolerance tests. Blood samples were drawn at specific time periods following ingestion of 100 grams of each carbohydrate. Blood was then analyzed for glucose and phagocytic index (the mean number of bacteria engulfed per leukocyte); a red blood cell and white blood cell count and differential white cell count were done also on about 1/2 of the samples. In addition, 7 other subjects participated in a fasting study in which no …

Cardioregulatory Role Of The Abdominal Ganglion Of Aplysia Californica, George Maeda

Loma Linda University Electronic Theses, Dissertations & Projects

An isolated heart-abdominal ganglion preparation of this mollusc was developed and used to study the cardioregulatory function of the ganglion and nerves. The heart was perfused with artificial sea water through an atrial cannula and the gastro-esophageal artery was catheterized for blood pressure monitoring. The presence and cardioregulatory effects of axons in the nerves was investigated by electrical stimulation. Fine-tipped glass microelectrodes inserted into ganglionic neurons were used to determine the relationship between the neuronal and cardiac activities.

The principal results are listed below.

1. Primarily inhibitory responses are obtained from both right and left connective stimulation, with some evidence for …

An Anesthetic Technique Utilizing Innovar, Fentanyl, And Methohexital Sodium For Outpatient Oral Surgery, Fredric D. Haerich

Loma Linda University Electronic Theses, Dissertations & Projects

A study of 800 patients ranging in age from seven to one hundred years was conducted in the development of an anesthetic technique which combines the desirable qualities of a neuroleptic agent with the requirements of an outpatient oral surgical anesthetic. These requirements included rapidity of onset, effectiveness of action, ease of controlling duration, and rapid postoperative recovery.

Patients who were cleared medically for outpatient procedures were given Innovar 1.5 cc/150 lb of body weight, atropine 0.4 mg, and fentanyl 1.0 cc/150 lb of body weight as an initial dose within 30-45 seconds in a D5W drip by means of …

Relationship Of Ventilation Ability And Positioning In Left-Sided Congestive Heart Failure Patients, Nancy E. Carson

Loma Linda University Electronic Theses, Dissertations & Projects

Proper positioning is used to relieve dyspnea, edema, and fatigue in congestive heart failure patients. According to the review of literature, improved ventilation as measured by specific lung volumes and lung mechanics occurred with upright positioning in normal persons. The effects of specific changes in lung volumes related to position change in congestive heart failure patients has not been documented in literature, although various recommendations related to positioning are made. This exploratory study was undertaken with nine congestive heart failure patients to see if a relationship existed between degree of head elevation and lung ventilation ability as measured by water-bell …

The Parenchymal Cells Of The Rat Pineal Gland

Faculty, Staff and Students Publications

Background: Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is a rare hematologic disorder with improved survival due to advancements in treatment. However, long-term cardiovascular morbidity and mortality remain significant. Established cardiovascular risk calculators, such as the 2008 Framingham Heart Study (FHS) global cardiovascular disease (CVD) and the American College of Cardiology/American Heart Association (ACC/AHA) atherosclerotic CVD (ASCVD) risk estimators, may not adequately account for the elevated and unique cardiovascular risks in iTTP survivors.

Objectives: To evaluate the discrimination and calibration of the ACC/AHA ASCVD and FHS global CVD models in predicting major adverse cardiovascular events (MACEs) among iTTP survivors.

Methods: This retrospective …

A Follow-Up Study Of Patients With Chronic Obstructive Lung Disease And Their Practice Of The Breathing Pattern, A. Theodora Djagia

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this descriptive survey was to do a follow-up study on patients with COPD to find out to what extent these patients have followed instruction to continue exercising the abdominal and diaphragmatic muscles, with the utilization of a breathing pattern. Literature was reviewed which revealed contradictory findings in regard to the physiological benefits of the use of the breathing pattern. Nine patients met the criteria and signed a consent sheet to be visited in their homes. An Observation Data Sheet and Interview Guide were the tools used to collect data. A point system was constructed and used with …

A Study To Demonstrate The Immediate Destination Of An Embolus Formed In The Lumen Of A Peripheral Venous Catheter, Rosemarie Morikone Emori

Loma Linda University Electronic Theses, Dissertations & Projects

This study was designed to show the immediate destination of an embolus formed in the lumen of an intravenous catheter placed in a peripheral vessel. The hypothesis stated that clots injected into a peripheral vessel will lodge in the lungs. A subhypothesis was that the clot may lodge anywhere along the circulatory pathway.

Indications of need for the study as shown by the review of literature were the following: (1) the complications resulting from the frequent use of intravenous polyethylene catheters, (2) experimental studies that showed that clots were formed around intravenous catheters, (3) the evidence that intravenous catheters predispose …

A Comparative In Vitro Metabolic Study Of Certain Pesticidal Phenyl N-Methyl-Carbamates, Larry A. Wheeler

Loma Linda University Electronic Theses, Dissertations & Projects

Two critical parameters in the evaluation of the toxicological hazards of the use of carbamate pesticides are: (1) a rapid, sensitive, and precise analytical method of identification of the carbamate and its metabolites; and (2) their metabolism. A method in this study was developed whereby various carbamates and their metabolites could be chromatographed in the gaseous phase. Also, a comparative in vitro study of 15,000 g liver supernatants from human, rat, and dog was performed. Additional tissues investigated for metabolic activity toward the carbamates, Zectran and Mesurol, were kidney (from the dog only) and various blood fractions (red blood cells, …

The Effect Of Exercise On Metabolic Rate With Time In Overweight And Normal Weight Subjects, Roberta Jewell Keswick

Loma Linda University Electronic Theses, Dissertations & Projects

Previous studies in the Lome Linda University laboratory showed that obese subjects on an exercise program lost approximately one pound more per week as compared to a non-exercise group. Energy expenditure calculations of the exercise did not account for the total caloric deficit required to lose the additional pound. The present study was designed to investigate changes in BMR and metabolic rate with time for normal weight and overweight subjects involved in a practical exercise program. The relationship of changes in BMR and metabolic rate in coping with the problems of obesity were also studied.

Fifteen subjects, eight overweight and …

Some Aspects Of Carbohydrate Metabolism In The Cercariae Of Schistosoma Mansoni Sambon, 1907, A. Eugene Dunham Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

Cercariac of Schistosoma mansoni were studied for glycogen utilization, uptake of glucose from solution, lactic acid production, and respiration.

Using the anthrone method of determination of carbohydrate, the amount of glycogen used by a single cercaria in 4 hours is determined as 43.2 x 10^-4 μg ± 6.6 x 10^-4 μg.

Glucose was not observed to have been used from solution under the conditions of the experiment, even in the presence of trehalose. The determinations were made by the Glucostat method.

After deproteinization and desugarization, aliquots of supernatant from cercarial suspensions were analyzed for lactic acid using p-phenylphenol …

The Effects Of Particular Sounds On Certain Indices Of Cardio-Pulmonary Functions, Harold F. Googe, Jane Mundin

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to identify the effects of particular sounds upon certain indices of cardio-pulmonary function and the degree and pattern of changes which occurred in the indices. The specific physiological parameters chosen to be monitored were heart rate, respiratory rate, mean tidal volume, instantaneous minute volume, and oxygen consumption. The hypothesis was that particular sounds will cause changes in certain indices of cardio-pulmonary functions. This was a controlled experiment, in which nine healthy males and nine healthy females, of a denominational, medically oriented University listened to a specially constructed tape of background music interspersed with an …