Digital Commons Network^™

Escape From Diabetes, J E. Jochum, J Carmack, J Toon

Clinical Practice in Athletic Training

Introduction: In the past decade, interprofessional education (IPE) to promote interprofessional collaborative practice has become an integral component of pre-licensure education in the health professions. Research shows that IPE activities involving problem-solving in patient care that requires cooperation and communication among the professions is most effective. An effective activity to meet interprofessional competencies is the use of escape rooms. Escape rooms are team-based games where groups of individuals solve puzzles to accomplish a specific goal, typically in a limited amount of time. There are many benefits to using escape rooms in education, including promoting problem-solving, teamwork, and creativity, improving focus …

Benefits Of Equilibrium Between Microbiota- And Host-Derived Ligands Of The Aryl Hydrocarbon Receptor After Stroke In Aged Male Mice, Pedram Peesh, Maria P Blasco-Conesa, Ahmad El Hamamy, Romeesa Khan, Gary U Guzman, Parisa Honarpisheh, Eric C Mohan, Grant W Goodman, Justin N Nguyen, Anik Banerjee, Bryce E West, Kyung Ae Ko, Janelle M Korf, Chunfeng Tan, Huihui Fan, Gabriela D Colpo, Hilda Ahnstedt, Lucy Couture, Solji Roh, Julia K Kofler, Jose F Moruno-Manchon, Michael E Maniskas, Jaroslaw Aronowski, Rodney M Ritzel, Juneyoung Lee, Jun Li, Robert M Bryan, Anjali Chauhan, Venugopal Reddy Venna, Louise D Mccullough, Bhanu Priya Ganesh

Faculty, Staff and Student Publications

Recent studies have highlighted the crucial role of microglia (MG) and their interactions with the gut microbiome in post-stroke neuroinflammation. The activation of immunoregulatory pathways, including the aryl hydrocarbon receptor (AHR) pathway, is influenced by a dynamic balance of ligands derived from both the host and microbiota. This study aimed to investigate the association between stroke-induced dysbiosis and the resultant imbalance in AHR ligand sources (loss of microbiota-derived [indole-based] and increase of host-derived [kynurenine-based]) after stroke. Microbiota-derived AHR ligands decreased in human plasma and remained low for days following an ischemic stroke highlighting the translational significance. Transient-middle-cerebral-artery-occlusion was performed in …

Identification Of Novel Genetic Biomarkers Linked To The Prodromal Stage Of Parkinson’S Disease (Pd) Patients, Marwa Tawfik Badawy

Theses and Dissertations

Background and Objective: The early diagnosis of neurodegenerative diseases, such as Parkinson's disease (PD), is particularly challenging because symptoms appear only after significant neuronal damage has already occurred. This study is utilizing variant call format (VCF) analysis to identify genetic variants and novel genes that could serve as early prognostic markers for prodromal PD. Materials and Methods: Data were sourced from the Parkinson's Progression Markers Initiative (PPMI), focusing on prodromal patients with gVCF data from the 2021 cohort. The study included 304 participants, comprising 100 healthy controls, 146 individuals with prodromal genetic indicators, 21 individuals with prodromal hyposmia, and 37 …

New Index For Distinguishing Between Critical Illness Myopathy And Neuromyopathy: Cmap Amplitude/Duration Index, Handan Uzunçakmak Uyanik, Fatma Gökçem Yildiz, Çağri Mesut Temuçi̇n

Turkish Journal of Medical Sciences

Background/Aim: Critical illness-associated weakness (CIAW) is a common neuromuscular syndrome that may manifest as critical illness myopathy (CIM), critical illness polyneuropathy (CIP), or an overlap syndrome known as critical illness polyneuromyopathy (CIPM). Distinguishing between these subtypes can be challenging due to technical issues, patient-related factors such as insufficient cooperation with needle electromyography (EMG) and edema, and the need for sophisticated, time-consuming electrophysiological methods and invasive procedures such as biopsy. This study aims to contribute to the electrophysiological distinction between CIM and CIPM. Materials and methods: A new index was calculated based on distal compound muscle action potential (CMAP) amplitude and …

Integrated Convolutional Neural Network For Skin Cancer Classification With Hair And Noise Restoration, Ni̇dhi̇ Bansal, Sri̇dhar Sundaramurthy

Turkish Journal of Medical Sciences

Background/aim: Skin lesions are commonly diagnosed and classified using dermoscopic images. There are many artifacts visible in dermoscopic images, including hair strands, noise, bubbles, blood vessels, poor illumination, and moles. These artifacts can obscure crucial information about lesions, which limits the ability to diagnose lesions automatically. This study investigated how hair and noise artifacts in lesion images affect classifier performance and how they can be removed to improve diagnostic accuracy.Materials and methods: A synthetic dataset created using hair simulation and noise simulation was used in conjunction with the HAM10000 benchmark dataset. Moreover, integrated convolutional neural networks (CNNs) were proposed for …

Relationship Between The Density Of Degenerated Neurons In The Carotid Body And Changes In Blood Ph, Hakan Kina, Aydin Si̇nan Apaydin

Turkish Journal of Medical Sciences

Background/aimCarotid artery is the majority artery for brain feeding. Carotid Bodies (CBs) are located in carotid bifurcation and regulate body fluid pH. We aimed to evaluate the changes in CB-related pH levels as a consequence of degeneration induced by the ligation of both Common Carotid Arteries (BCCAL).Materials and methodsWe included 23 adult male New Zealand rabbits. A control group (n= 5); a sham group (0.5 mL saline injected n=5) and the main study group (subjected to the BCCAL procedure, n = 13). We monitored and recorded pH levels, and cardiac rhythms before, during, and after the experiments at two-day intervals. …

Association Of Certain Biochemical Parameters Related To Bone Cycle With Genotype In Mps Iii-B Patients, Seda Gökkurt, İrem Peker Eyüboğlu, Banu Nur Güzel, Ercan Mihçi, Ayşe Özer, Mustafa Akki̇pri̇k

Turkish Journal of Medical Sciences

Background/aim: The aim of this study is to investigate the genotype-phenotype correlation in Sanfilippo type B patients in terms of bone formation/resorption parameters, and to determine the release/inhibition of biomarkers accompanying osteoporosis.Materials and Methods: Plasma levels of Osteoprotegerin, Matrix metalloproteinases (MMP2, MMP9), TIMP-1, TIMP-2, BALP, Cathepsin K, and TRACP-5b were examined by ELISA method in MPS IIIB patients. At the same time, mutations in the NAGLU gene causing this disease were detected by whole exome sequencing, and their correlation with biochemical parameters was investigated.Results: Based on the enzyme analysis results, MMP-9, TIMP1, TIMP-2, and MMP-2 were significantly high, while Cathepsin-K …

Thoracic Disc Herniations: Diagnosis, Surgical Techniques, And Complication Insights, Mehmet Deni̇zhan Yurtluk, Aydin Si̇nan Apaydin, Hakan Kina, Khoi Than

Turkish Journal of Medical Sciences

Abstract: Thoracic disc herniations (TDHs) are rare conditions, comprising 0.1% to 5% of all reported herniation cases, and affecting up to 1 in 1,000,000 individuals. The mid to lower thoracic spine, particularly between the T11 and T12 vertebrae, is most frequently affected and often associated with trauma. Conditions like Scheuermann’s disease can predispose patients to TDHs. This study explores the clinical manifestations, diagnostic methods, surgical techniques, and complications associated with TDHs. Diagnosis relies on magnetic resonance imaging and computed tomography myelography to identify the level and nature of the herniation. Various surgical approaches, including posterolateral, lateral, and anterior, have been …

Impact Of Concurrent Parathyroid Adenoma On The Recurrence Of Differentiated Thyroid Carcinoma: A Retrospective Study, Derya Çayir, Alev Çinar, Mehmet Bozkurt, Bahadir Külah

Turkish Journal of Medical Sciences

Background/aim: The impact of concurrent parathyroid adenoma in differentiated thyroid carcinoma remains unclear. This study was performed to compare the recurrence rates of patients operated on for differentiated thyroid carcinoma with concomitant parathyroid adenoma and those operated on for thyroid carcinoma without detected parathyroid adenoma.Materials and methods: This retrospective study examined 340 patients who underwent total thyroidectomy for thyroid carcinoma at our institution. We compared the patients with concurrent parathyroid adenoma to those without, assessing clinical, laboratory, surgical, and histopathological findings and thyroid carcinoma recurrence over a 6-year postoperative period.Results: Among all patients, 53/340 (16%) were identified with parathyroid adenoma, …

A Comparison Study Of Sensory Processing In Older Adults With And Without Dementia, Medi̇ne Nur Özata Değerli̇, Di̇dem Karaduman, Burcu Balam Doğu, Onur Altuntaş

Turkish Journal of Medical Sciences

Background/aim: Sensory processing is affected by aging, and dementia may lead to changes in sensory processing. This study aimed to examine the differences in sensory processing and sensory modulation between older adults with and without dementia.Materials and methods: This study was designed as a comparative study of community-dwelling older adults aged 65 years and older with and without dementia. Participants and their caregivers were recruited at the occupational therapy clinic of a public hospital. They completed a sociodemographic information form and the Adolescent/Adult Sensory Profile to assess sensory processing and sensory modulation.Results: The study included 120 older adults (60 with …

Novel Nonmagnetic Abutment Designs For Facial Prostheses: An Experimental Study, İrem Sozen Yani̇k, Ufuk Adali̇, Jamila Yassine, Fransizka Schmidt, Wolfgang Hannak, Bahadir Ersu

Turkish Journal of Medical Sciences

Background/aim: This in vitro study aimed to evaluate and compare the retentive forces of novel nonmagnetic abutment designs developed as an alternative to conventional magnetic abutments for facial prostheses.Materials and methods: A plexiglass model was constructed and two extraoral implants were placed in these blocks in a parallel position. Nonmagnetic abutments made of titanium were fabricated and screwed onto the implants. The nonmagnetic systems represent a novel design and include two different abutment designs (Type 1 and Type 2) with silicone attachments. Retentive force values for three abutment types: Conventional Magnetic System (CMS), Nonmagnetic abutment Type 1 System (NMS1), and …

Relationship Between Uncoupling Protein 1 (Ucp1) Levels And Psoriasis, Fi̇kret Akyurek, Fatma Tuncez Akyurek, Fatma Sengul

Turkish Journal of Medical Sciences

Background/aim: Psoriasis is an autoimmune, chronic and common skin disease. Comorbidities also increase the mortality risk of the disease. The aim of this study is to investigate the changes in UCP1 levels in psoriasis patients and to evaluate their possible role in the pathogenesis of the disease, focusing on disease severity (Psoriasis Area and Severity Index), dyslipidemia, inflammation and cardiovascular risk.Materials and methods: This study included 30 psoriasis patients and 30 healthy individuals as a control group. Serum UCP1 was measured using an ELISA test kit. The laboratory results of psoriasis patients and healthy controls were compared.Results: UCP1 level was …

Does Femoral Tunnel Location Have An Effect On Functional And Clinical Results In Medial Patellofemoral Ligament Reconstruction?, İbrahi̇m Kaya, Murat Çi̇çekli̇dağ, Resul Bi̇rcan, Mustafa Odluyurt, Abdurrahman Vural, İnci̇ Hazal Ayas, Muhammet Baybars Ataoğlu, Ulunay Kanatli

Turkish Journal of Medical Sciences

Background/aimMultiple reconstruction techniques have been described to mimic the normal anatomy and physiology of medial patellofemoral ligament reconstruction (MPFL). The success of the MPFL depends on various factors such as graft selection, the location of the patellar tunnel and femoral tunnel (FT), and graft fixation methods. The aim of this study is to analyze the relationship of femoral tunnel location with functional and clinical outcomes after MPFL reconstruction.Materials and methodsIn this study, the mid-term clinical results of patients who underwent MPFL reconstruction for patellofemoral instability in a single institution between 2013 and 2019 were evaluated retrospectively. If the FT was …

The Abdominal Compliance Index And Postoperative Pain After Laparoscopic Gynecologic Surgery: A Preliminary Observational Cohort Study, Ebru Akgün Şarer, Mustafa Tahtaci, Umut Cahi̇t Ersoy, Mehtap Honca

Turkish Journal of Medical Sciences

Background/aimLaparoscopic surgery in gynecology has advantages of reduced scarring, less postoperative pain, faster recovery and lower risk of complications. Despite these adavantages, shoulder pain still remains a major concern affecting upto 90% of patients undergoing gynecologic laparoscopic surgery. The use of Abdominal compliance index (ACI) may offer the benefits of gaining work space for safer surgery and may prevent the drawbacks of applying higher insuflation pressures.We investigated the relation of shoulder pain and abdominal pain and analgesic use with the recently advised ACI which is a surrogate index of abdominal expansion in patients undergoing gynecologic laparoscopic surgery.Materials and methodsThirty one …

Erratum To “ Telemedicine In Pediatric Rheumatology: The Video Pediatric Gait, Arms, Legs, And Spine (V-Pgals) Examination” [Turkish Journal Of Medical Sciences 54 (5) 2024 963-969 ], Zeynep Balik, Seher Şener, Yağmur Bayindir, Müşerref Kasap Cüceoğlu, Emi̇l Ali̇yev, Özge Başaran, Yelda Bi̇lgi̇ner, Seza Özen, Ezgi̇ Deni̇z Batu

Turkish Journal of Medical Sciences

This erratum is written to correct an error in our previous publication. Supplement Figure 2 was published incorrectly as one page instead of six.
DOI of original article: https://doi.org/10.55730/1300-0144.5874

Machine Learning-Enhanced Surface-Enhanced Spectroscopic Detection Of Polycyclic Aromatic Hydrocarbons In The Human Placenta, Oara Neumann, Yilong Ju, Andres B Sanchez-Alvarado, Guodong Zhou, Weiwu Jiang, Bhagavatula Moorthy, Melissa A Suter, Ankit Patel, Peter Nordlander, Naomi J Halas

Faculty, Staff and Students Publications

The detection and identification of polycyclic aromatic hydrocarbons (PAHs) and their derivatives, polycyclic aromatic compounds (PACs), are essential for environmental and health monitoring, for assessing toxicological exposure and their associated health risks. PAHs/PACs are the most dangerous chemicals found in tobacco smoke, and cigarette use during pregnancy can convey these molecules to the developing fetus through the placenta. This exposure is associated with many negative health outcomes, from premature birth to sudden infant death syndrome and adverse neurodevelopmental disorders. This study demonstrates the use of surface-enhanced Raman and surface-enhanced infrared absorption spectroscopies for direct detection of PAHs/PACs in human placental …

Trends In Ischemic Stroke Hospitalization And Outcomes In The United States Pre- And Peri-Covid-19 Pandemic: A National Inpatient Sample Study, Alibay Jafarli, Mario Di Napoli, Rachel S Kasper, Jeffrey L Saver, Louise D Mccullough, Setareh Salehi-Omran, Behnam Mansouri, Vasileios Arsenios Lioutas, Mohammed Ismail, Afshin A Divani

Faculty, Staff and Student Publications

Background/Objectives: The COVID-19 pandemic impacted healthcare systems globally, disrupting the management and treatment of acute ischemic stroke (AIS). Understanding how AIS admissions, treatments, and outcomes were affected is critical for improving stroke care in future crises. The objective of this work was to assess the COVID-19 pandemic's impact on AIS admissions, treatment patterns, complications, and patient outcomes in the U.S. from 2016 to 2021, focusing on differences between pre-pandemic (2016-2019) and peri-pandemic (2020-2021) periods.

Methods: This is a retrospective cohort study using the National Inpatient Sample (NIS) database, analyzing weighted discharge records of AIS patients over six years. Data encompass …

The Potential Of Using Screening Tools For Bipolar Disorder To Predict Lithium Response, Delfina Janiri, Mario Pinto, Silvia Montanari, Ester Maria Marzo, Greta Sfratta, Guglielmo Donofrio, Alexia Koukopoulos, Giovanni Camardese, Alessio Simonetti, Lorenzo Moccia, Gianna Autullo, Gabriele Sani

Faculty, Staff and Students Publications

Background/Objectives: Lithium is the gold standard for treating Bipolar Disorder (BD), but its effectiveness varies widely. While clinical and environmental factors may influence response, it remains unclear if screening tools can reliably predict lithium response outcomes. This study explores this potential using two widely used screening instruments for BD.

Methods: A total of 146 patients with BD were evaluated. Lithium response was assessed using the Alda Scale, while hypomanic and manic symptoms were characterized through the Hypomania Checklist-32 (HCL-32) and the Mood Disorder Questionnaire (MDQ). Group differences in HCL-32 and MDQ scores were analyzed using ANOVA, and a multivariate model …

Single-Cell Analysis Of Neoplastic Plasma Cells Identifies Myeloma Pathobiology Mediators And Potential Targets, Luz Yurany Moreno Rueda, Hua Wang, Keiko Akagi, Minghao Dang, Amishi Vora, Li Qin, Hans C Lee, Krina K Patel, Pei Lin, David E Mery, Fenghuang Zhan, John D Shaughnessy, Qing Yi, Yang Song, Bo Jiang, Maura L Gillison, Sheeba K Thomas, Donna M Weber, Lixia Diao, Jing Wang, Isere Kuiatse, Elisabet E Manasanch, David E Symer, Robert Z Orlowski

Faculty, Staff and Student Publications

Multiple myeloma is a clonal plasma cell (PC) dyscrasia that arises from precursors and has been studied utilizing approaches focused on CD138+ cells. By combining single-cell RNA sequencing (scRNA-seq) with scB-cell receptor sequencing (scBCR-seq), we differentiate monoclonal/neoplastic from polyclonal/normal PCs and find more dysregulated genes, especially in precursor patients, than we would have by analyzing bulk PCs. To determine whether this approach can identify oncogenes that contribute to disease pathobiology, mitotic arrest deficient-2 like-1 (MAD2L1) and S-adenosylmethionine synthase isoform type-2 (MAT2A) are validated as targets with drug-like molecules that suppress myeloma growth in preclinical models. Moreover, functional studies show a …

Mitochondrial Defects And Metabolic Vulnerabilities In Lynch Syndrome-Associated Msh2-Deficient Endometrial Cancer, Mikayla Borthwick Bowen, Brenda Melendez, Qian Zhang, Diana Moreno, Leah Peralta, Wai Kin Chan, Collene Jeter, Lin Tan, M Anna Zal, Philip L Lorenzi, Kenneth Dunner, Richard K Yang, Russell R Broaddus, Joseph Celestino, Nisha Gokul, Elizabeth Whitley, Deena M Scoville, Tae Hoon Kim, Jae-Wook Jeong, Rosemarie Schmandt, Karen Lu, Hyun-Eui Kim, Melinda S Yates

Faculty, Staff and Student Publications

Lynch syndrome (LS), caused by inherited mutations in DNA mismatch repair genes, including MSH2, carries a 60% lifetime risk of developing endometrial cancer (EC). Beyond hypermutability, mechanisms driving LS-associated EC (LS-EC) remain unclear. We investigated MSH2 loss in EC pathogenesis using a mouse model (PR-Cre Msh2LoxP/LoxP, abbreviated Msh2KO), primary cell lines, human tissues, and human EC cells with isogenic MSH2 knockdown. By 8 months, 58% of Msh2KO mice developed endometrial atypical hyperplasia (AH), a precancerous lesion. At 12-16 months, 50% of Msh2KO mice exhibited either AH or ECs with histologic similarities to human LS-ECs. Transcriptomic profiling of EC from Msh2KO …

A New Formula For Predicting The Actual Volume Of Parathyroid Adenoma In Patients With Primary Hyperparathyroidism, İbrahi̇m Kilinç, Mustafa Oruç, Serap Ulusoy, Ali̇ Coşkun, Mehmet Kiliç

Turkish Journal of Medical Sciences

Backround/AimKnowing the adenoma volume in patients with primary hyperparathyroidism (PHPT) is useful for surgeons during parathyroidectomy. The primary aim of our study was to determine how to calculate adenoma volume with the greatest accuracy. Our secondary aim was to determine the relationship between adenoma volume and preoperative biochemical parameters (parathyroid hormone [PTH] and calcium levels).Material and MethodsWe prospectively collected the medical history of 75 patients who underwent surgery for a single parathyroid adenoma. After excision, the adenomas were placed in water-filled syringes and the actual volume was determined according to the amount of water displaced. The radiological volume of the …

Assessing Knowledge Levels Of Intensive Care Unit Nurses And Doctors Regarding Drug Administration Via Enteral Feeding Tubes: A Survey Study, Ayşe Gül Koçoğlu Kinal, Yunus Emre Ayhan, Aslinur Albayrak

Turkish Journal of Medical Sciences

Background/aimKnowledge deficiency regarding appropriate drug administration through enteral feeding tubes (EFTs). is common in intensive care units (ICUs). The aim of this study is to evaluate the knowledge levels of nurses and doctors in ICUs about drug administration via EFTs.Materials and methodsThis study was conducted as a cross-sectional online survey. Survey questions were created using Google Forms and distributed to nurses and doctors in various ICUs across hospitals in Istanbul, Türkiye. The researchers designed the survey questions based on literature reviews and existing examples. The survey consisted of three sections and a total of 25 questions: the first section included …

Navigating Pregnancy With Cardiovascular Disease: Pathophysiology, Risk Stratification, And Maternal–Fetal Outcomes, Osman Türkmen, Serra Akar İnan

Turkish Journal of Medical Sciences

Pregnancy induces significant cardiovascular changes, including increases in heart rate, blood volume, and cardiac output, which may exacerbate existing heart conditions or unmask previously undiagnosed heart diseases.. This review outlines the types of cardiovascular diseases that affect pregnant women, organized by the modified World Health Organization risk classification. It covers key areas, including how pregnancy impacts the heart, common maternal and fetal complications, and effective management strategies. The review emphasizes the importance of preconception counseling, risk assessment, and continuous monitoring to ensure the best maternal and fetal outcomes. It also highlights practical guidelines for healthcare providers and discusses the latest …

Unveiling The Genetic Landscape Of Coronary Artery Disease Through Common And Rare Structural Variants, Kruthika R Iyer, Shoa L Clarke, Rodrigo Guarischi-Sousa, Ketrin Gjoni, Adam S Heath, Erica P Young, Nathan O Stitziel, Cecelia Laurie, Jai G Broome, Alyna T Khan, Joshua P Lewis, Huichun Xu, May E Montasser, Kellan E Ashley, Natalie R Hasbani, Eric Boerwinkle, Alanna C Morrison, Nathalie Chami, Ron Do, Ghislain Rocheleau, Donald M Lloyd-Jones, Rozenn N Lemaitre, Joshua C Bis, James S Floyd, Gregory L Kinney, Donald W Bowden, Nicholette D Palmer, Emelia J Benjamin, Matthew Nayor, Lisa R Yanek, Brian G Kral, Lewis C Becker, Sharon L R Kardia, Jennifer A Smith, Lawrence F Bielak, Arnita F Norwood, Yuan-I Min, April P Carson, Wendy S Post, Stephen S Rich, David Herrington, Xiuqing Guo, Kent D Taylor, Joann E Manson, Nora Franceschini, Katherine S Pollard, Braxton D Mitchell, Ruth J F Loos, Myriam Fornage, Lifang Hou, Bruce M Psaty, Kendra A Young, Elizabeth A Regan, Barry I Freedman, Ramachandran S Vasan, Daniel Levy, Rasika A Mathias, Patricia A Peyser, Laura M Raffield, Charles Kooperberg, Alex P Reiner, Jerome I Rotter, Goo Jun, Paul S De Vries, Themistocles L Assimes

Faculty, Staff and Student Publications

Background: Genome-wide association studies have identified several hundred susceptibility single nucleotide variants for coronary artery disease (CAD). Despite single nucleotide variant-based genome-wide association studies improving our understanding of the genetics of CAD, the contribution of structural variants (SVs) to the risk of CAD remains largely unclear.

Method and results: We leveraged SVs detected from high-coverage whole genome sequencing data in a diverse group of participants from the National Heart Lung and Blood Institute's Trans-Omics for Precision Medicine program. Single variant tests were performed on 58 706 SVs in a study sample of 11 556 CAD cases and 42 907 controls. …

An Integrative Multiparametric Approach Stratifies Putative Distinct Phenotypes Of Blast Phase Chronic Myelomonocytic Leukemia, Kristian Gurashi, Yu-Hung Wang, Fabio M R Amaral, Katherine Spence, Rachel Cant, Chi-Yuan Yao, Chien-Chin Lin, Christopher Wirth, David C Wedge, Guillermo Montalban-Bravo, Simona Colla, Hwei-Fang Tien, Tim C P Somervaille, Kiran Batta, Daniel H Wiseman

Faculty, Staff and Student Publications

Approximately 30% of patients with chronic myelomonocytic leukemia (CMML) undergo transformation to a chemo-refractory blastic phase (BP-CMML). Seeking novel therapeutic approaches, we profiled blast transcriptomes from 42 BP-CMMLs, observing extensive transcriptional heterogeneity and poor alignment to current acute myeloid leukemia (AML) classifications. BP-CMMLs display distinctive transcriptomic profiles, including enrichment for quiescence and variability in drug response signatures. Integrating clinical, immunophenotype, and transcriptome parameters, Random Forest unsupervised clustering distinguishes immature and mature subtypes characterized by differential expression of transcriptional modules, oncogenes, apoptotic regulators, and patterns of surface marker expression. Subtypes differ in predicted response to AML drugs, validated ex vivo in …

Single-Cell Rna Sequencing Identifies Molecular Biomarkers Predicting Late Progression To Cdk4/6 Inhibition In Patients With Hr+/Her2- Metastatic Breast Cancer, Linjie Luo, Peng Yang, Sofia Mastoraki, Xiayu Rao, Yan Wang, Nicole M Kettner, Akshara Singareeka Raghavendra, Debasish Tripathy, Senthil Damodaran, Kelly K Hunt, Jing Wang, Ziyi Li, Khandan Keyomarsi

Faculty, Staff and Student Publications

Background: Cyclin-dependent kinase 4/6 inhibitors (CDK4/6is) in combination with endocrine therapy are the standard treatment for patients with hormone receptor-positive, HER2-negative metastatic breast cancer (mBC). Despite the efficacy of CDK4/6is, intrinsic resistance occurs in approximately one-third of patients, highlighting the need for reliable predictive biomarkers.

Methods: Single-cell RNA sequencing analyzed metastatic tumors from HR+/HER2- mBC patients pre-CDK4/6i treatment at baseline (BL) and/or at disease progression. BL samples were from CDK4/6i responders (median progression-free survival [mPFS] = 25.5 months), while progressors were categorized as early-progressors (EP, mPFS = 3 months) and late-progressors (LP, mPFS = 11 months). Metastatic sites included liver, …

Outcomes Of Arteriovenous Malformation Patients With Multiple Versus Single Feeders: A Multicenter Retrospective Study With Propensity-Score Matching, Basel Musmar, Nimer Adeeb, Hammam Abdalrazeq, Joanna M Roy, Stavropoula I Tjoumakaris, Hamza Adel Salim, Douglas Kondziolka, Jason Sheehan, Christopher S Ogilvy, Howard Riina, Sandeep Kandregula, Adam A Dmytriw, Kareem El Naamani, Ahmed Abdelsalam, Natasha Ironside, Deepak Kumbhare, Cagdas Ataoglu, Muhammed Amir Essibayi, Abdullah Keles, Sandeep Muram, Daniel Sconzo, Arwin Rezai, Omar Alwakaa, Salem M Tos, Ufuk Erginoglu, Johannes Pöppe, Rajeev D Sen, Christoph J Griessenauer, Jan-Karl Burkhardt, Robert M Starke, Mustafa K Baskaya, Laligam N Sekhar, Michael R Levitt, David J Altschul, Malia Mcavoy, Assala Aslan, Abdallah Abushehab, Christian Swaid, Adib A Abla, Saman Sizdahkhani, Sravanthi Koduri, Elias Atallah, Spyridon Karadimas, M Reid Gooch, Robert H Rosenwasser, Christopher Stapleton, Matthew Koch, Visish M Srinivasan, Peng R Chen, Spiros Blackburn, Joseph Cochran, Omar Choudhri, Bryan Pukenas, Darren Orbach, Edward Smith, Pascal J Mosimann, Ali Alaraj, Mohammad A Aziz-Sultan, Aman B Patel, Hugo H Cuellar, Michael Lawton, Bharat Guthikonda, Jacques Morcos, Pascal Jabbour

Faculty, Staff and Student Publications

Introduction: The impact of multiple feeding arteries on clinical outcomes of cerebral arteriovenous malformations (AVMs) is not well understood. This study aims to compare outcomes between AVMs with multiple versus single feeding arteries.

Patients and methods: Data from the Multicenter International Study for Treatment of Brain AVMs (MISTA) consortium were analyzed. Propensity score matching (PSM) was used to balance cohorts. Subgroup analysis was conducted for ruptured and unruptured AVMs and different treatment options, and multivariable logistic regression was applied to assess the impact of feeding artery origin.

Results: Among 953 patients, 661(69.4%) had multiple feeding arteries, and 292 (30.6%) had …

Mage-A4 Induces Non-Small Cell Lung Cancer And Tumor-Promoting Plasma Cell Accumulation, Dominique Armstrong, Cheng-Yen Chang, Monica J Hong, Linda Green, Yichao Shen, William Hudson, Kelsey E Mauk, Li-Zhen Song, Sheetal Jammi, Benjamin Casal, Brianna Burns, Chad J Creighton, Alexandre Carisey, Xiang H-F Zhang, Neil J Mckenna, Sung Wook Kang, Hyun-Sung Lee, William Decker, David B Corry, Farrah Kheradmand

Faculty, Staff and Students Publications

Adaptive immunity is critical in eliminating tumors, but cancer-intrinsic factors can subvert this function. Melanoma antigen-A4 (MAGE-A4), a cancer-testis antigen, is expressed in solid tumors and correlates with poor survival, but its role in tumorigenesis and antitumor immunity remains unclear. We found that expression of MAGE-A4 was highly associated with the loss of PTEN, a tumor suppressor, in human non–small cell lung cancers (NSCLC). Here, we show that constitutive expression of human MAGE-A4 with Pten loss in mouse airway epithelia results in metastatic adenocarcinoma. Tumors showed distinct enrichment in IgA+ CD138+ CXCR4+ plasma cells (PCs) and increased expression of …

Multimodal Objective Assessment Of Impulsivity In Healthy And Mood Disorder Participants, Bishal Lamichhane, Nidal Moukaddam, Ramiro Salas, Wayne Goodman, Ashutosh Sabharwal

Faculty, Staff and Students Publications

Impulsivity represents an individual's tendency to act on urges without sufficient forethought. Heightened impulsivity is a hallmark of many mental health disorders. Objective impulsivity assessments could improve risk evaluation, diagnosis, and behavioral outcome monitoring in impulsivity-related health disorders. Towards objective impulsivity assessment, in this work, we identify impulsivity correlates in objective measurements, investigate their complementarity, and contrast impulsivity mechanisms across health conditions. We analyzed behavioral tests, heart rate variability (HRV), and fMRI-based brain connectivity in 227 healthy participants and 34 participants with mood disorders. Impulsivity dimensions had complementary correlates in objective measurements, with fMRI providing the strongest correlates. Multimodal assessment …

Identification Of Distinct Stool Metabolites In Women With Endometriosis For Non-Invasive Diagnosis And Potential For Microbiota-Based Therapies, Chandni Talwar, Goutham Venkata Naga Davuluri, Abu Hena Mostafa Kamal, Cristian Coarfa, Sang Jun Han, Surabi Veeraragavan, Krishna Parsawar, Nagireddy Putluri, Kristi Hoffman, Patricia Jimenez, Scott Biest, Ramakrishna Kommagani

Faculty, Staff and Students Publications

Background: Endometriosis, a poorly studied gynecological condition, is characterized by the presence of ectopic endometrial lesions resulting in pelvic pain, inflammation, and infertility. These associated symptoms contribute to a significant burden, often exacerbated by delayed diagnosis. Current diagnostic methods involve invasive procedures, and existing treatments provide no cure.

Methods: Microbiome-metabolome signatures in stool samples from individuals with and without endometriosis were determined using unbiased metabolomics and 16S bacteria sequencing. Functional studies for selected microbiota-derived metabolites were conducted in vitro using patient-derived cells and in vivo by employing murine and human xenograft pre-clinical disease models.

Findings: We discovered a unique bacteria-derived …